Bioelectrochemistry最新文献_第2页

In-situ integrated Ce-MOF-808@CeO2 as bifunctional matrix for sensitive electrochemical-aptasensing of tetracycline in shrimp

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-03-03 DOI: 10.1016/j.bioelechem.2025.108965

Wenjing Xu, Yanan Zhao, Feng Gao, Xuan Zheng, Fengping Zhan, Qingxiang Wang

{"title":"In-situ integrated Ce-MOF-808@CeO2 as bifunctional matrix for sensitive electrochemical-aptasensing of tetracycline in shrimp","authors":"Wenjing Xu, Yanan Zhao, Feng Gao, Xuan Zheng, Fengping Zhan, Qingxiang Wang","doi":"10.1016/j.bioelechem.2025.108965","DOIUrl":"10.1016/j.bioelechem.2025.108965","url":null,"abstract":"<div><div>The integration of aptamer chemistry with innovative functional materials such as nanozymes offers new opportunities for the development of the superior electrochemical biosensors. Herein, we introduce a rod-like nanocomposite of Ce-MOF-808@CeO<sub>2</sub> bearing intense nanozymatic activity that prepared through in-situ partial oxidation of Ce-MOF-808 to CeO<sub>2</sub>. Then, the aptamer for tetracycline (TC-Apt) with 5’-PO<sub>4</sub><sup>3−</sup> end was anchored on Ce-MOF-808@CeO<sub>2</sub> modified screen-printed electrode, thereby assembling a label-free electrochemical aptasensor. Electrochemical and spectroscopic assays reveal that the derived CeO<sub>2</sub> can effectively promote the nanozyme activity of Ce-MOF-808 as a cocatalyst. Electrochemical biosensing shows that, the capture of tetracycline (TC) to the electrode surface by the aptamer chemistry significantly inhibits the catalytic activity of Ce-MOF-808@CeO<sub>2</sub>. Thus, TC can be analyzed by monitoring the catalytic signal of the biosensor to H<sub>2</sub>O<sub>2</sub>. Leveraging the exceptional catalytic activity of Ce-MOF-808@CeO<sub>2</sub>, coupled with the high specificity of the aptamer, TC can be analyzed in a wide kinetic range from 1 pM to 100 nM, with a low detection limit of 0.21 pM. The aptasensor is also applicable for the accurate detection of TC residues in fresh shrimp samples, showcasing its potential for practical applications in the monitoring of food safety.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108965"},"PeriodicalIF":4.8,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143563045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phthalocyanine based metal organic frameworks for electrochemical detection of human epidermal growth factor receptor 2

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-03-03 DOI: 10.1016/j.bioelechem.2025.108966

Lunathi Ncwane , Philani Mashazi , Tebello Nyokong

{"title":"Phthalocyanine based metal organic frameworks for electrochemical detection of human epidermal growth factor receptor 2","authors":"Lunathi Ncwane , Philani Mashazi , Tebello Nyokong","doi":"10.1016/j.bioelechem.2025.108966","DOIUrl":"10.1016/j.bioelechem.2025.108966","url":null,"abstract":"<div><div>Herein, we report on the development of a phthalocynaine based metal organic frameworks (MOF) for the detection of human epidermal growth factor receptor 2 (HER2). Phthalocyanines (Pcs) exhibit good redox properties, hence their utilization as precursors for the synthesis of Pc based MOFs. The successful preparation of the MOF was confirmed using X-ray powder diffraction (XRD), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) spectroscopy, X-ray photoelectron spectroscopy (XPS), and Brunner Emmet Teller (BET) analysis. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were utilized for electrochemical characterization of the Co octacarboxy Pc (CoOCPc), and Co-CoOCPc-MOF modified glassy carbon electrode (GCE). Differential pulse votammetry was employed for detection of HER2, which is a biomarker for cancer. The selectivity towards HER2 biomarker was accomplished by attaching an aptamer (Apt) onto the MOF modified glassy carbon surface. The GCE/Co-CoOCPc-MOF/Nf/Apt (Nf = Nafion) showed excellent analytical parameters with lowest limit of detection of 5.4 × 10 ng/mL, good repeatability and stability.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108966"},"PeriodicalIF":4.8,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143562867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The introduction of surface oxygen groups on fluid-like electrodes enhances biofilm growth of Geobacter sulfurreducens allowing continuous operation

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-03-01 DOI: 10.1016/j.bioelechem.2025.108963

María Llorente , Abraham Esteve-Núñez , Raúl Berenguer

{"title":"The introduction of surface oxygen groups on fluid-like electrodes enhances biofilm growth of Geobacter sulfurreducens allowing continuous operation","authors":"María Llorente , Abraham Esteve-Núñez , Raúl Berenguer","doi":"10.1016/j.bioelechem.2025.108963","DOIUrl":"10.1016/j.bioelechem.2025.108963","url":null,"abstract":"<div><div>Microbial Electrochemical Fluidized Reactors (ME-FBR) changed the paradigm for growing electroactive bacteria from a biofilm strategy to a planktonic mode, while still performing direct extracellular electron transfer from oxidative metabolism in absence of redox mediators. Glassy carbon was the material selected for growing planktonic <em>Geobacter sulfurreducens</em> in ME-FBR. However, the material was unable to retain cells so applications implying continuous operation have been compromised. In this context, a tailor-made chemical strategy was followed considering the large amount of cytochromes C present on the outermost membrane of bacteria form of the <em>Geobacter</em> genus. In this work, a commercial glassy carbon (GC) was chemically modified with surface oxygen groups (SOGs) mainly carboxylic type with high affinity for heme group of cytochrome C. The functionalized material did conserve the structural and textural features and i) promoted the biofilm formation of <em>Geobacter</em> using acetate as sole carbon and electron donor source, and ii) increased the current density and acetate removal rate in comparison with pristine carbon. Thus, the new material enriched in carboxylic-type SOGs facilitates <em>a-la-carte</em> anchorage of electroactive bacteria to move on from a planktonic-based to a biofilm-based strategy, so ME-FBR operation could be expanded from batch to continuous mode, while electrical current was still possible.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108963"},"PeriodicalIF":4.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel hybrid biosensor for miRNA detection based on peptide nucleic acids and molecularly imprinted polymers

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-03-01 DOI: 10.1016/j.bioelechem.2025.108964

Muhammed Abdel-Hamied , Min Guo , Yiming Wei , Joachim Bansmann , Rasha M. El Nashar , Franz Oswald , Boris Mizaikoff , Christine Kranz

{"title":"A novel hybrid biosensor for miRNA detection based on peptide nucleic acids and molecularly imprinted polymers","authors":"Muhammed Abdel-Hamied , Min Guo , Yiming Wei , Joachim Bansmann , Rasha M. El Nashar , Franz Oswald , Boris Mizaikoff , Christine Kranz","doi":"10.1016/j.bioelechem.2025.108964","DOIUrl":"10.1016/j.bioelechem.2025.108964","url":null,"abstract":"<div><div>The detection of miRNAs serving as key biomarkers in cancer diagnostics is challenging due to their small size, low abundance, and high sequence similarity, which complicates their sensitive and selective detection. Here, we report a biosensor that combines molecularly imprinted polymers (MIPs) with peptide nucleic acids (PNAs) to achieve a sensitive and highly selective miRNA detection in RNA isolates of cancer cells. MIPs were synthesized by electropolymerization utilizing PNA-supported and pre-oriented miR-21 templates molecules, which serve as both a linker for improved template orientation and an assistive recognition element for miRNA binding. Electrochemical impedance spectroscopy (EIS) was used to detect miR-21 after optimization of the sensor architecture and the experimental parameters. The sensor exhibited excellent sensitivity and selectivity toward miR-21 even when compared to the single mismatched sequence, with a linear response of 0.5–5000 pM and a limit of detection (LoD) of 0.11 ± 0.04 pM without any amplification steps. The sensor was used to quantify miR-21 in artificial serum and in RNA isolates of cancer cells to discriminate between MCF-7 and Hela cells. This approach opens new avenues for the application of MIPs as synthetic antibodies in miRNA research and emphasizes the importance of the synergistic integration of PNA.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108964"},"PeriodicalIF":4.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143551712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Imaging functional bacterial pore-forming toxins in tethered bilayer lipid membranes using scanning electrochemical microscopy

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-02-28 DOI: 10.1016/j.bioelechem.2025.108953

Tomas Sabirovas , Milda Pleckaityte , Wojciech Nogala , Vaishali Shrivastav , Rima Budvytyte

{"title":"Imaging functional bacterial pore-forming toxins in tethered bilayer lipid membranes using scanning electrochemical microscopy","authors":"Tomas Sabirovas , Milda Pleckaityte , Wojciech Nogala , Vaishali Shrivastav , Rima Budvytyte","doi":"10.1016/j.bioelechem.2025.108953","DOIUrl":"10.1016/j.bioelechem.2025.108953","url":null,"abstract":"<div><div>This study demonstrates the straightforward application of scanning electrochemical microscopy (SECM) for characterizing pneumolysin-induced pores in tethered bilayer lipid membranes (tBLMs). Carbon-based nanoelectrodes with a tip radius of approximately 20 nm produced distinct feedback responses during approach curves to the sample. A positive feedback response was observed when approaching the self-assembled monolayer, while the few nanometers thick tBLMs exhibited characteristics of insulating layers, yielding a negative feedback response. Based on the computational calculations, the reconstitution of functional pneumolysin was further confirmed through electrochemical impedance spectroscopy, with a concentration of 5 nM pneumolysin resulting in an average pore density of 0.64 μm<sup>−2</sup>. Finally, we demonstrated the practical utility of SECM for visualizing pneumolysin pores within the tBLM system. These experiments highlight the versatility and cost-effectiveness of electrochemical techniques for investigating membrane integrity, toxin activity, and biomolecular interactions at the nanoscale.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108953"},"PeriodicalIF":4.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-mode DNA nano-stage biosensing platform for efficient detection of uracil-DNA glycosylase activity in cells

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-02-26 DOI: 10.1016/j.bioelechem.2025.108950

Dandan Hu , Yanhong Ye , Qianlin Zhu , Ruyue Cong , Jingran Sun , Kaiyue Hu , Yufang Hu

{"title":"Dual-mode DNA nano-stage biosensing platform for efficient detection of uracil-DNA glycosylase activity in cells","authors":"Dandan Hu , Yanhong Ye , Qianlin Zhu , Ruyue Cong , Jingran Sun , Kaiyue Hu , Yufang Hu","doi":"10.1016/j.bioelechem.2025.108950","DOIUrl":"10.1016/j.bioelechem.2025.108950","url":null,"abstract":"<div><div>Analyzing uracil-DNA glycosylase (UDG) activity is essential for understanding DNA repair mechanisms in disease progression and treatment. This study presents a dual-mode DNA nano-stage biosensing platform integrating electrochemiluminescence (ECL) and electrochemical impedance spectroscopy (EIS) for highly sensitive and specific UDG detection. A DNA-prism-modified electrode immobilizes UDG-responsive elements, forming a stable and efficient detection interface. Upon UDG cleavage, released DNA fragments initiate rapid nano-stage assembly, significantly amplifying the signal output. ECL signals are produced by embedded [Ru(phen)<sub>3</sub>]<sup>2+</sup> complexes, while EIS signals result from the reaction of 3,3′-diaminobenzidine (DAB) with H<sub>2</sub>O<sub>2</sub>, catalyzed by manganese tetrakis(4-<em>N</em>-methylpyridyl)porphyrin (MnTMPyP). The platform achieves an exceptional detection limit of 1.0 × 10<sup>−5</sup> U/mL, effectively validating the inhibitory effects of UDG inhibitors. Furthermore, a strong correlation between UDG activity and HeLa cell number is demonstrated. Compared to a commercial UDG detection kit, the biosensor exhibits comparable sensitivity with enhanced versatility. Notably, UDG activity is significantly higher in cancerous cells than in normal cells, reflecting the increased DNA repair demand in malignancy. This capability to distinguish UDG activity among different cell types highlights its potential for cancer diagnostics, while this biosensor platform shows promise for broader applications in clinical diagnostics, cancer research, and drug discovery.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108950"},"PeriodicalIF":4.8,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143519898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial.

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-02-25 DOI: 10.1016/j.bioelechem.2025.108947

Wlodzimierz Kutner

引用次数: 0

In situ growth of two-dimensional MXene/Nano-copper metal-organic framework composites for antimicrobial applications in epoxy coatings

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-02-25 DOI: 10.1016/j.bioelechem.2025.108952

Fuyan Kang , Guangyu Zhu , Xiaoya Ding , Yabei Zhang , Zilong Zhao , Tao Zhang , Zhongyi He , Fa-Qian Liu

{"title":"In situ growth of two-dimensional MXene/Nano-copper metal-organic framework composites for antimicrobial applications in epoxy coatings","authors":"Fuyan Kang , Guangyu Zhu , Xiaoya Ding , Yabei Zhang , Zilong Zhao , Tao Zhang , Zhongyi He , Fa-Qian Liu","doi":"10.1016/j.bioelechem.2025.108952","DOIUrl":"10.1016/j.bioelechem.2025.108952","url":null,"abstract":"<div><div>Marine biofouling and corrosion are serious impediments to the promotion and development of the marine industry. The short service life and limited application of single coatings have greatly increased the economic burden on the industry. The development of multi-functional composite coatings has become a particularly pressing issue. The combination of Cu-BTC and Ti<sub>3</sub>C<sub>2</sub>Tx as a specific resin filler represents a new strategy (Ti<sub>3</sub>C<sub>2</sub>Tx@Cu-BTC@EP). HAADF-STEM, PXRD and XPS were used to verify the successful synthesis of the materials. Ti<sub>3</sub>C<sub>2</sub>Tx@Cu-BTC@EP was able to achieve 100 % lethality of <em>E. coli</em> under the condition of light exposure within 24 h. In addition, the impedance modulus of the coating in the low-frequency range was increased by about 3.15 times compared to the blank group with the addition of 1 wt% filler, reaching as high as 7.06 × 10<sup>8</sup> Ω. Overall, the novel Ti<sub>3</sub>C<sub>2</sub>Tx@Cu-BTC@EP composite coating is expected to promote new advances in epoxy resin research.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108952"},"PeriodicalIF":4.8,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143508785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of flexible nanoporous gold electrodes for the detection of glucose

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-02-24 DOI: 10.1016/j.bioelechem.2025.108949

Denise Demurtas , Julia Alvarez-Malmagro , Arvind Rathore , Tanushree Mandal , Andrés Felipe Quintero-Jaime , Serguei Belochapkine , Anna Lielpetere , Kavita Jayakumar , Dónal Leech , Wolfgang Schuhmann , Nicolas Mano , Claudine Boiziau , Edmond Magner

{"title":"Development of flexible nanoporous gold electrodes for the detection of glucose","authors":"Denise Demurtas , Julia Alvarez-Malmagro , Arvind Rathore , Tanushree Mandal , Andrés Felipe Quintero-Jaime , Serguei Belochapkine , Anna Lielpetere , Kavita Jayakumar , Dónal Leech , Wolfgang Schuhmann , Nicolas Mano , Claudine Boiziau , Edmond Magner","doi":"10.1016/j.bioelechem.2025.108949","DOIUrl":"10.1016/j.bioelechem.2025.108949","url":null,"abstract":"<div><div>The development of implantable glucose sensors is of significant interest in the management of diabetes. This work focuses on developing an implantable, biocompatible nanoporous gold electrode prototype based on Kapton® for the subcutaneous detection of glucose. The electrodes were first modified with a layer containing glucose oxidase and Os(2,2′-bipyridine)<sub>2</sub>Cl·PVI (Os(bpy)<sub>2</sub>Cl PVI). An additional polymeric layer <em>co</em>ntaining poly(2-methacryloyloxyethyl phosphorylcholine-co-glycidyl methacrylate) was then added to reduce biofouling and foreign body reaction effects. The modified electrode had a V<sub>MAX</sub> of 211 ± 13 μA cm<sup>−2</sup> and a K<sub>Mapp</sub> of 6.1 ± 0.8 mM in pseudo physiological conditions, with a linear detection range from 1 to 4 mM and a sensitivity of 28.6 ± 2.1 μA cm<sup>−2</sup> mM<sup>−1</sup>. In artificial plasma, the response of the sensor was saturated at 3 mM, with a V<sub>MAX</sub> of 113 ± 10 μA cm<sup>−2</sup> and a K<sub>Mapp</sub> of 2.1 ± 0.4 mM with a linear detection range from 1 to 2.5 mM and a sensitivity of 14.6 ± 3.3 μA cm<sup>−2</sup> mM<sup>−1</sup>. Mechanical stress testing demonstrated that there was a 40 % reduction of the redox polymer coverage after 320 deformation events, however the catalytic activity was still detectable after 160 events. Minimal cytotoxicity effects of the electrodes were observed. When subcutaneously implanted the electrodes showed fairly good mechanical stability after one week and detachment of the metallic layer on some electrodes after 21 days, probably due to electrode bending. A limited foreign body reaction was observed. These results indicated that the electrodes could be implanted for a period of up to 1 week.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108949"},"PeriodicalIF":4.8,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143551711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modelling enzyme electrodes – What do we learn and how is it useful?

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-02-22 DOI: 10.1016/j.bioelechem.2025.108941

Philip N. Bartlett, M. Hashim Khan

引用次数: 0