Bioelectrochemistry最新文献_第2页

Highly water-retaining conductive hydrogels based on multi-crosslinked networks for ultrasensitive sensing platform 基于多交联网络的高保水导电水凝胶用于超灵敏传感平台

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2025-09-23 DOI: 10.1016/j.bioelechem.2025.109120

Jiaqing Wang , Youyu Li , Ning Li , Hongliang Han , Zhanfang Ma , Haijun Yang

{"title":"Highly water-retaining conductive hydrogels based on multi-crosslinked networks for ultrasensitive sensing platform","authors":"Jiaqing Wang , Youyu Li , Ning Li , Hongliang Han , Zhanfang Ma , Haijun Yang","doi":"10.1016/j.bioelechem.2025.109120","DOIUrl":"10.1016/j.bioelechem.2025.109120","url":null,"abstract":"<div><div>Hydrogels are widely used in electrochemical sensors due to their unique properties, but their conductivity, influenced by water-content, is highly susceptible to external environment. Therefore, enhancing the water-retention of hydrogels while ensuring stable conductivity and analytical performance is crucial for broadening their application. In this work, a novel polyacrylamide/bacterial cellulose/sulfobetaine methacrylate/sodium alginate composite hydrogel (PBSS)-based multi-crosslinked network hydrogel was designed. The water retention of the PBSS hydrogel was improved by a factor of 1.5 compared to the unreinforced polyacrylamide (PAM) hydrogel (Water loss of hydrogel exposed for 12 h at 37 °C). With the water retention properties of itself, the PBSS hydrogel retained 86 % of its initial conductivity after 12 h of exposure at 60 °C, whereas the PAM hydrogel not only exhibited poor initial conductivity but also lost up to 47 % of its conductivity. PBSS hydrogels were designed as sensing platforms and CaCO<sub>3</sub> spheres were designed as immunoprobes. Ca<sup>2+</sup> released by the probe rivals Ni<sup>2+</sup> for the signaling substance on the substrate, enabling the quantification of the target analyte. The sensor exhibited excellent analytical performance and maintained stable performance after four days of storage at 37 °C, offering a promising approach to enhance hydrogel sensor stability for clinical applications.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109120"},"PeriodicalIF":4.5,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A colorimetric/electrochemical dual-mode aptasensor for Golgi protein 73 detection using TMB as a bifunctional probe 以TMB为双功能探针的高尔基蛋白73检测比色/电化学双模适体传感器

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2025-09-23 DOI: 10.1016/j.bioelechem.2025.109121

Guiyin Li , Jing Su , Min Chen , Xiaohong Tan , Lingling Fan , Zhide Zhou , Chong Hu , Jintao Liang

{"title":"A colorimetric/electrochemical dual-mode aptasensor for Golgi protein 73 detection using TMB as a bifunctional probe","authors":"Guiyin Li , Jing Su , Min Chen , Xiaohong Tan , Lingling Fan , Zhide Zhou , Chong Hu , Jintao Liang","doi":"10.1016/j.bioelechem.2025.109121","DOIUrl":"10.1016/j.bioelechem.2025.109121","url":null,"abstract":"<div><div>Golgi protein 73 (GP73) has emerged as a critical biomarker for the diagnosis of hepatocellular carcinoma (HCC), and its detection is essential for the effective treatment of this disease. In this study, we developed a colorimetric/electrochemical dual-mode aptasensor for GP73 detection using a hemin-reduced graphene oxide‑manganese oxide (H-rGO-Mn<sub>3</sub>O<sub>4</sub>) nanozyme and the bifunctional probe, 3,3′,5,5′-tetramethylbenzidine (TMB). The GP73-specific aptamer (Apt) was conjugated to the H-rGO-Mn<sub>3</sub>O<sub>4</sub> nanozyme, which was then immobilized on the surface of a gold nanoparticle-modified screen-printed electrode (Au NPs/SPE) through hybridization with a complementary DNA strand (cDNA) attached to the electrode. TMB served as a dual-signal probe for both colorimetric and electrochemical detection. In the presence of GP73, Apt binds specifically to the target, forming a H-rGO-Mn<sub>3</sub>O<sub>4</sub>-Apt/GP73 complex structure. This binding event causes the nanozymes to detach from the electrode surface, reducing its peroxidase activity while simultaneously restoring the electrochemical signal. Under optimized conditions, the dual-mode aptasensor exhibited excellent linear responses for GP73 concentrations ranging from 0.0001 to 100 ng/mL, with limits of detection (LOD) of 0.1 pg/mL for both the colorimetric and electrochemical modes. The aptasensor was successfully applied to measure GP73 levels in serum samples, yielding highly consistent results. Its performance was further validated by comparison with the standard ELISA method. Compared to single-signal aptasensors, this dual-mode approach not only improves detection sensitivity but also minimizes the risk of false positives or false negatives, making it a more reliable tool for accurate GP73 detection in clinical diagnostics.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109121"},"PeriodicalIF":4.5,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrolytically generated size-tunable micro-nano bubbles toward high-efficiency antibacterial applications in vitro 电解生成尺寸可调微纳气泡的体外高效抗菌应用

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2025-09-19 DOI: 10.1016/j.bioelechem.2025.109117

Qiang Mao , Kai Wei , Tianyu Li , Jiasheng Zhu , Huarui Han , Kan Hu , Changchang Ma , Sheng Feng

{"title":"Electrolytically generated size-tunable micro-nano bubbles toward high-efficiency antibacterial applications in vitro","authors":"Qiang Mao , Kai Wei , Tianyu Li , Jiasheng Zhu , Huarui Han , Kan Hu , Changchang Ma , Sheng Feng","doi":"10.1016/j.bioelechem.2025.109117","DOIUrl":"10.1016/j.bioelechem.2025.109117","url":null,"abstract":"<div><div>Bacterial contamination in water poses severe threats to global water safety, particularly in drinking water. Micro-nano bubbles (MNBs) have attracted significant interest for antibacterial applications due to their high efficiency and environmental friendliness. In this study, size-tunable MNBs were electrochemically fabricated using TiO<sub>2</sub> nanotube arrays (TNTAs) as electrodes. A strong positive correlation (R<sup>2</sup> = 0.965) was established between the pore diameter of TNTAs and the size of the generated MNBs, enabling precise control over bubble dimensions. Crucially, bactericidal efficiency was found to be negatively correlated with MNB size, with the smallest MNBs achieving exceptional inactivation rates of 98.8 % for <em>Escherichia coli (E. coli)</em> and 98.0 % for <em>Staphylococcus aureus (S. aureus)</em>. Mechanistic studies revealed that smaller MNBs generate higher concentrations of hydroxyl radicals (•OH) upon collapse, which are primarily responsible for the enhanced antibacterial activity. This work establishes a clear structure–activity relationship: electrode pore size dictates MNB size, which in turn determines •OH generation and antibacterial performance. These findings provide a scientific basis for developing efficient, environmentally friendly disinfection technologies.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109117"},"PeriodicalIF":4.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nafion-stabilized silver nanoparticles modified glassy carbon electrode for ultrasensitive detection of alpha-1-acid glycoprotein 钠稳定的纳米银修饰玻碳电极用于α -1-酸性糖蛋白的超灵敏检测

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2025-09-19 DOI: 10.1016/j.bioelechem.2025.109112

Gulam Rabbani , Akbar Mohammad , Mohammad Ehtisham Khan , Waleed Zakri , Mohsin Vahid Khan , Khurshid Ahamd , Wahid Ali , Syed Kashif Ali , Nazim Hasan , Abdulrahman Khamaj , Jintae Lee

{"title":"Nafion-stabilized silver nanoparticles modified glassy carbon electrode for ultrasensitive detection of alpha-1-acid glycoprotein","authors":"Gulam Rabbani , Akbar Mohammad , Mohammad Ehtisham Khan , Waleed Zakri , Mohsin Vahid Khan , Khurshid Ahamd , Wahid Ali , Syed Kashif Ali , Nazim Hasan , Abdulrahman Khamaj , Jintae Lee","doi":"10.1016/j.bioelechem.2025.109112","DOIUrl":"10.1016/j.bioelechem.2025.109112","url":null,"abstract":"<div><div>In this study, alpha-1-acid glycoprotein (AGP), a crucial biomarker associated with various diseases and physiological conditions, was selected for detection using the developed immunosensor. The immunosensor was fabricated by depositing a synthesized Nafion/silver nanoparticles (AgNPs) nanocomposite on the glassy carbon electrode as the substrate material. The changes in the physicochemical properties after Nafion/AgNPs nanocomposite deposition were analyzed through SEM and XPS measurements. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to analyze the immunosensor fabrication's electrochemical performance. AGP was detected through the immobilized anti-AGP on the electrode surface. The developed immunosensor exhibited a wide linear detection range from 0.05 to 3.2 mg/mL and a LOD of 0.17 mg/mL. The developed immunosensor showed excellent selectivity and repeatability when subjected to different kinds of interfering proteins. The recovery of AGP ranged from 101.7 to 103.7 % and RSD was <4 %, indicating high accuracy in real samples detection. The immunosensor developed for AGP detection showed a number of beneficial characteristics, including low cost and small volume sample requirements, making it highly suitable for point-of-care applications. This study provides new insights into the precise fabrication and affordable immunosensors for diverse clinical diagnostic applications.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109112"},"PeriodicalIF":4.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An electrochemical aptasensor based on synergistic effect of duplex-specific nuclease and nanoporous gold for ultrasensitive detection of prostate-specific antigen 基于双特异性核酸酶和纳米孔金协同作用的电化学配体传感器用于前列腺特异性抗原的超灵敏检测

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2025-09-19 DOI: 10.1016/j.bioelechem.2025.109113

Xiaohui Wang , Xinran Li , Yanxia Xie , Hongshuai Fang , Xueru Cui , Jianwei Jiao , Yachao Zhang

{"title":"An electrochemical aptasensor based on synergistic effect of duplex-specific nuclease and nanoporous gold for ultrasensitive detection of prostate-specific antigen","authors":"Xiaohui Wang , Xinran Li , Yanxia Xie , Hongshuai Fang , Xueru Cui , Jianwei Jiao , Yachao Zhang","doi":"10.1016/j.bioelechem.2025.109113","DOIUrl":"10.1016/j.bioelechem.2025.109113","url":null,"abstract":"<div><div>Prostate cancer (PCa), the most common solid tumor in men, is typically suspected based on digital rectal examination and elevated serum prostate-specific antigen (PSA) levels. Given its clinical significance, developing a PSA biosensing system with high sensitivity and stability is essential. In this study, a novel aptasensor, leveraging the synergistic effect of duplex specific nuclease (DSN) and nanoporous gold (NP-Gold), was firstly developed for PSA detection. The aptamer, which hybridizes with its complementary DNA to form double-stranded DNA (dsDNA), was used as biorecognition element. NP-Gold served as the supporting material. By exploiting the large specific surface area and high conductivity of NP-Gold, the immobilized amount of dsDNA was increased, thereby expanding the dynamic detection range of PSA. DSN can recognize and cleave non-PSA-bound dsDNA. By reducing noise signals derived from such unbound dsDNA, it enhances the correlation between electrical signals and PSA concentration, ultimately lowering the detection limit. Benefiting from the synergistic effect of DSN and NP-Gold, the developed aptasensor showed a wide linear relationship between current values and PSA concentrations, with a detection range from 100 fg/mL to 10 ng/mL and a limit of detection (LOD) of 2.7 fg/mL. Moreover, the aptasensor demonstrated excellent resistance to interference from various proteins and retained 96 % of its current response after 21 days of storage. Additionally, the proposed aptasensor successfully detected PSA concentrations in real human serum samples, with accuracy comparable to that of the ELISA kit. These distinctive characteristics made the aptasensor a reliable choice for PSA detection and early diagnosis of PCa.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109113"},"PeriodicalIF":4.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated methane microbial fuel cell system for concurrent nitrogen and sulfur removal through autotrophic cathodic bioreduction 通过自养阴极生物还原同时去除氮和硫的集成甲烷微生物燃料电池系统

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2025-09-19 DOI: 10.1016/j.bioelechem.2025.109116

Jing Lu, Xinyu Li, Yating Jia, Liuyi Cai, Xinrui Wu, Shuqing Zheng, Bin Hou

{"title":"Integrated methane microbial fuel cell system for concurrent nitrogen and sulfur removal through autotrophic cathodic bioreduction","authors":"Jing Lu, Xinyu Li, Yating Jia, Liuyi Cai, Xinrui Wu, Shuqing Zheng, Bin Hou","doi":"10.1016/j.bioelechem.2025.109116","DOIUrl":"10.1016/j.bioelechem.2025.109116","url":null,"abstract":"<div><div>The cathodic reduction of nitrate and sulfate by autotrophic organisms in microbial fuel cells (MFC) has received much attention. However, systematic investigations into the feasibility of methane-driven nitrate and sulfate reduction remain limited. In this study, an MFC integrating an autotrophic biocathode with dissolved methane (CH<sub>4</sub>) as the electron donor was developed to couple methane-driven reduction of nitrate and sulfate with electricity generation. The biology reductive properties, microbial characteristics and functional metabolic mechanisms were investigated under single electron acceptor (NO<sub>3</sub><sup>−</sup>/SO<sub>4</sub><sup>2−</sup>) and mixed electron acceptor (NO<sub>3</sub><sup>−</sup> + SO<sub>4</sub><sup>2−</sup>) working conditions. The results showed that with nitrate and sulfate acting as electron acceptors alone, MFC achieved the maximum removal rates of nitrate and sulfate of 89.1 % and 26 %, respectively. With mixed electron acceptors provided, the removal rates of nitrate and sulfate decreased by 62.5 % and 14.2 %, respectively. However, the anaerobic oxidation of methane (AOM) was promoted, and its output voltage reached a maximum. The anode chambers of all methane autotrophic denitrification‑sulfur removing MFCs shared similar microbial structures, with dominant functional genera including <em>Methylocystis</em>, <em>Hyphomicrobium</em> and <em>Methylomonas</em>, and the dominant bacteria in the cathode chamber were <em>Pseudomonas</em>, <em>Nitrospira</em>, <em>Desulfovibrio</em>, <em>Hyphomicrobium</em> and <em>Acidovorax</em>. The genes coding for methane metabolism were upregulated when nitrate and sulfate coexisted, while the genes related to sulfur metabolism and denitrification metabolism were downregulated. These findings provide novel insights into the application of AOM-MFC systems for the treatment of wastewater with nitrogen and sulfur contaminants.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109116"},"PeriodicalIF":4.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new strategy for assessing chemo cardiotoxicity risk based on CRP recognition and the application of novel sensing technology 基于CRP识别和新型传感技术应用的化疗心脏毒性风险评估新策略

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2025-09-18 DOI: 10.1016/j.bioelechem.2025.109110

Bolu Sun , Haiying He , Zixia Wang , Yuqiong Wu , Jiali Kang , Xinlan Wang , Xuanxiu Da , Miao Zhou , Ying Lv , Chenyu Qin , Xiangdong Wang , Lin Yang , Huajie Li , Hai Wang

{"title":"A new strategy for assessing chemo cardiotoxicity risk based on CRP recognition and the application of novel sensing technology","authors":"Bolu Sun , Haiying He , Zixia Wang , Yuqiong Wu , Jiali Kang , Xinlan Wang , Xuanxiu Da , Miao Zhou , Ying Lv , Chenyu Qin , Xiangdong Wang , Lin Yang , Huajie Li , Hai Wang","doi":"10.1016/j.bioelechem.2025.109110","DOIUrl":"10.1016/j.bioelechem.2025.109110","url":null,"abstract":"<div><div>Cancer chemotherapy, while enhancing patient survival, is limited by cardiotoxicity. Early monitoring and risk assessment are essential. C-reactive protein (CRP) can serve as an early biomarker for predicting cardiotoxicity during chemotherapy, offering a critical opportunity to develop new cardioprotective strategies. This study used an anti-CRP antibody loaded onto an electrode modified with chitosan (CS) and functionalized carbon black (f-CB) as a target. Due to f-CB's excellent electrical conductivity and CS's high permeability and strong adhesion, an immunosensor for the early monitoring and risk assessment of chemotherapy-induced cardiotoxicity based on CRP-specific immunorecognition was successfully constructed. Under optimal experimental conditions, the immunosensor exhibited excellent linearity in the range of 3.9 × 10<sup>−1</sup> to 4 × 10<sup>2</sup> ng mL<sup>−1</sup> with a detection limit as low as 3.16 × 10<sup>−1</sup> ng mL<sup>−1</sup>, making it more sensitive than ELISA. The immunosensor also demonstrated excellent selectivity, reproducibility, and stability in detecting CRP in a chemotherapy-induced cardiotoxicity model, with recovery rates ranging from 97.14 % to 107.65 %. This study presents an efficient, accurate, and practical approach for the early detection and risk assessment of chemotherapy-induced cardiotoxicity, thereby supporting a balance between therapeutic efficacy and cardiac safety.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109110"},"PeriodicalIF":4.5,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of microbiologically influenced corrosion in 90/10 CuNi alloy by a novel antibacterial peptide 新型抗菌肽对90/10 CuNi合金微生物腐蚀的抑制作用

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2025-09-16 DOI: 10.1016/j.bioelechem.2025.109114

Nianting Xue, Hao Sun, Shihang Lu, Yizhen Yu, Wenyan Ma, Guangzhou Liu, Wenwen Dou

{"title":"Inhibition of microbiologically influenced corrosion in 90/10 CuNi alloy by a novel antibacterial peptide","authors":"Nianting Xue, Hao Sun, Shihang Lu, Yizhen Yu, Wenyan Ma, Guangzhou Liu, Wenwen Dou","doi":"10.1016/j.bioelechem.2025.109114","DOIUrl":"10.1016/j.bioelechem.2025.109114","url":null,"abstract":"<div><div>Microbiologically influenced corrosion (MIC) poses a serious threat to the structural integrity of Cu<img>Ni alloys under marine service conditions. This study proposed a novel antibacterial peptide (Tcs) combined with Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) to inhibit MIC in 90/10 Cu<img>Ni alloy induced by <em>Desulfovibrio ferrophilus</em> IS5. Bacterial counts, environmental parameters analysis, surface morphological analyses, weight loss measurements, and electrochemical tests were conducted, through which the antibacterial and corrosion inhibition effects were demonstrated. The results showed that 180 ppb Peptide Tcs (180 Tcs) alone exerted almost no inhibitory effect on <em>D. ferrophilus</em> IS5, whereas a significant concentration-dependent enhancement was observed when Peptide Tcs was combined with 20 ppm ADBAC (20 ADBAC). Specifically, the 20 ppm ADBAC +180 ppb Peptide Tcs (20 ADBAC-180 Tcs) combination further reduced sessile bacterial counts by one order of magnitude compared to 20 ADBAC alone, while the weight loss rate and maximum pitting depth decreased by approximately 86 % and 61 %, respectively. These findings provide new insights into optimizing traditional biocide systems with small antimicrobial peptides and developing green, efficient strategies for controlling marine MIC.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109114"},"PeriodicalIF":4.5,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spectroelectrochemical studies of TDMQ20: A potential drug against Alzheimer's disease - part 2 - Cu-complexes 抗阿尔茨海默病潜在药物TDMQ20的光谱电化学研究——第二部分- cu络合物。

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2025-09-16 DOI: 10.1016/j.bioelechem.2025.109115

Martin Perez-Estebanez , Fabiola Olmo-Alonso , Natalia Baran , Martin Jönsson-Niedziółka , Magdalena Z. Wiloch

{"title":"Spectroelectrochemical studies of TDMQ20: A potential drug against Alzheimer's disease - part 2 - Cu-complexes","authors":"Martin Perez-Estebanez , Fabiola Olmo-Alonso , Natalia Baran , Martin Jönsson-Niedziółka , Magdalena Z. Wiloch","doi":"10.1016/j.bioelechem.2025.109115","DOIUrl":"10.1016/j.bioelechem.2025.109115","url":null,"abstract":"<div><div>Alzheimer's Disease (AD) has been associated with the overproduction reactive radicals through the catalytic effect of β-amyloid peptide Cu(II) complexes. In this article, we performed electrochemical characterisation of copper complexed with TDMQ20, which has been suggested as a potential drug against AD, using electrochemistry and spectroelectrochemistry (SEC). Based on voltammetry, we showed that oxidation as well as reduction processes of Cu(II)-TDMQ20 are pH-sensitive. Simulations of the CVs allowed us to estimate the kinetic parameters of the initial quasi-reversible oxidation of Cu(II) and the subsequent internal electron transfer to the Cu(III) ion from the TDMQ20 ligand (k<sub>0</sub> ≈ 18 s<sup>−1</sup>). The electrochemical mechanism of oxidation/reduction of Cu(II)-TDM20 complex was also studied using UV-VIS absorption spectroelectrochemistry (UV-VIS SEC). UV-VIS SEC measurements showed that after oxidation of the ligand through the reduction of the Cu(III), the Cu remains complexed with the TDMQ20, but the electrochemical properties of the complex are changed. Our findings contribute to a broader understanding of the redox activity of the Cu(II)-TDMQ20 complex at physiological pH, which is a crucial aspect in evaluating the potential clinical utility of this compound.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109115"},"PeriodicalIF":4.5,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145084631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a highly efficient DNA-based biosensor using a functionalized MoS₂/polypyrrole nanocomposite for mitomycin C determination 基于功能化MoS 2 /聚吡咯纳米复合材料的高效dna生物传感器的开发用于丝裂霉素C的测定。

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2025-09-13 DOI: 10.1016/j.bioelechem.2025.109111

Afsaneh Mousa Pour , Zahra Garkani-Nejad , Hadi Mahmoudi-Moghaddam

{"title":"Development of a highly efficient DNA-based biosensor using a functionalized MoS₂/polypyrrole nanocomposite for mitomycin C determination","authors":"Afsaneh Mousa Pour , Zahra Garkani-Nejad , Hadi Mahmoudi-Moghaddam","doi":"10.1016/j.bioelechem.2025.109111","DOIUrl":"10.1016/j.bioelechem.2025.109111","url":null,"abstract":"<div><div>In this study, plate-like Eu<sup>3+</sup>-doped MoS₂ nanoparticles (PL-Eu<sup>3+</sup>-doped MoS₂ NPs) were successfully synthesized via a simple hydrothermal method and characterized using energy-dispersive X-ray spectroscopy (EDX), field-emission scanning electron microscopy (FESEM), and X-ray diffraction (XRD). Subsequently, a novel electrochemical DNA biosensor was developed by modifying pencil graphite electrodes (PGEs) with the synthesized nanocomposite and polypyrrole (PPy) for the sensitive detection of mitomycin C (MC). The interaction between double-stranded DNA (ds-DNA) and MC was thoroughly investigated using differential pulse voltammetry (DPV), spectrophotometry, and molecular docking methods. The decrease in the oxidation peak currents of adenine base in an acetate buffer solution (pH 4.80) was employed as a reliable indicator of the interaction event. The constructed biosensor (ds-DNA/PL-Eu<sup>3+</sup>-doped MoS₂/PGE) exhibited a linear response toward MC concentrations ranging from 1 to 75 μg/mL, achieving a low detection limit of 0.8 μg/mL. The sensor's performance was further validated in real sample analyses, demonstrating excellent recovery values between 97 % and 104 %, thereby confirming its potential for practical applications in biomedical and environmental monitoring. UV/Vis spectroscopic data combined with molecular docking analyses indicated substantial interaction between mitomycin and DNA, with evidence supporting groove binding as the predominant binding mode.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109111"},"PeriodicalIF":4.5,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0