Self-assembled enzyme biosensors based on xanthine oxidase (XO) and CS@GO for screening natural products for anti-gout active ingredients

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2025-06-16 DOI:10.1016/j.bioelechem.2025.109032

Tong Gao , Bolu Sun , Bei Wang , Xuanxiu Da , Ying Lv , Miao Zhou , Xinlan Wang , Yuqiong Wu , Huajie Li , Haiying He , Jiali Kang , Xiangdong Wang , Chenyu Qin , Lin Yang

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Abstract

Gout is a joint disease caused by the deposition of monosodium urate, the incidence of which shows an escalating trend annually while demonstrating a propensity toward younger populations, whereas available pharmacological interventions frequently induce severe adverse effects. In this investigation, a highly sensitive electrochemical enzyme biosensor was constructed based on the pivotal role of xanthine oxidase (XO); the enzymatic activity was enhanced through chitosan - functionalized graphene oxide (CS@GO)-modified electrodes coupled with electrodeposited gold nanoparticles (AuNPs), which, when integrated with their superior electrical conductivity along with exceptional biocompatibility, facilitated the highly efficient screening of anti-gout components within natural products. Following optimization of critical parameters, evaluation of 14 compounds revealed five plant extracts (accounting for 28 % of samples) exhibiting XO inhibition exceeding 83 %, demonstrating superior efficacy compared to allopurinol (83.32 %). This methodology establishes an innovative strategy for rapid identification of highly potent yet minimally toxic anti-gout natural compounds, offering significant reference value regarding natural drug development.

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基于黄嘌呤氧化酶（XO）和CS@GO的自组装酶生物传感器用于筛选抗痛风活性成分的天然产物

痛风是一种由尿酸钠沉积引起的关节疾病，其发病率呈逐年上升趋势，且倾向于年轻人群，而现有的药物干预往往会引起严重的不良反应。本研究基于黄嘌呤氧化酶（xanthine oxidase， XO）的关键作用，构建了一种高灵敏度的电化学酶生物传感器；壳聚糖功能化氧化石墨烯（CS@GO）修饰电极与电沉积金纳米粒子（AuNPs）结合，增强了酶活性，当结合其优越的导电性和卓越的生物相容性时，促进了天然产物中抗痛风成分的高效筛选。通过优化关键参数，对14个化合物进行评价，发现5个植物提取物（占样品的28%）的XO抑制率超过83%，优于别嘌呤醇（83.32%）。该方法为快速鉴定高效且毒性最小的抗痛风天然化合物建立了一种创新策略，为天然药物开发提供了重要的参考价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.