Electrochemical-colorimetric dual-mode detection of zearalenone based on restriction endonuclease-driven DNA walker system

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2025-06-11 DOI:10.1016/j.bioelechem.2025.109029

Yanjun Wang , Zhenyuan Yang , Shaojie Xia , Junjie Huang , Yonghong Wang , Ge Ning

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引用次数: 0

Abstract

Zearalenone (ZEN) is a toxic metabolite produced mainly by strains of Fusarium spp. and is characterized by high toxicity and easy residue. Prolonged exposure to ZEN-contaminated grains can produce a range of toxic effects in the body and affect human health. In this study, an electrochemical and colorimetric dual-mode biosensor for ZEN detection was developed based on streptavidin-modified magnetic beads (SA-MBs) and the DNA walker. In this strategy, Apt/Walker could be used to specifically recognize ZEN and trigger two endonuclease-driven walker reactions, which resulted in the disruption and cleavage of orbital chains on SA-MBs and on the electrode surface. This ultimately led to dynamic changes in the colorimetric signal of the solution and the electrochemical signal on the electrode surface. The integration of target recognition with dual signal amplification and the reliability of dual mode detection were some of the advantages of the designed sensor. Under optimal conditions, the detection limits for both modes were 3.44 × 10⁻¹⁰ mol/L (colorimetry) and 3.39 × 10⁻⁹ mol/L (electrochemistry), respectively. At the same time, this dual-mode sensor had good specificity and recovery. It addressed the limitations of traditional detection methods and has broad potential in areas such as food safety.

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基于限制性内切酶驱动DNA walker系统的电化学-比色双模检测玉米赤霉烯酮

玉米赤霉烯酮（ZEN）是一种主要由镰刀菌产生的有毒代谢物，具有高毒性和易残留的特点。长期接触被禅宗污染的谷物会在体内产生一系列毒性作用，影响人体健康。在本研究中，基于链霉亲和素修饰磁珠（sa - mb）和DNA助行器开发了一种用于ZEN检测的电化学和比色双模生物传感器。在该策略中，Apt/Walker可以特异性识别ZEN并触发两个内切酶驱动的Walker反应，导致sa - mb和电极表面的轨道链断裂和断裂。这最终导致溶液的比色信号和电极表面的电化学信号发生动态变化。目标识别与双信号放大的结合以及双模式检测的可靠性是所设计传感器的优点。在最佳条件下，两种模式的检出限分别为3.44 × 10−10 mol/L（比色法）和3.39 × 10−9 mol/L（电化学法）。同时，该双模传感器具有良好的特异度和恢复能力。它解决了传统检测方法的局限性，在食品安全等领域具有广泛的潜力。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.