Optimizing electrolytic ablation therapy: Effects of electrode polarization modes through in silico and in vitro studies

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2025-08-05 DOI:10.1016/j.bioelechem.2025.109062

Enaide Maine Calzado , Nahuel Olaiz , Luis Enrique Bergues Cabrales , Pablo Turjanski

{"title":"Optimizing electrolytic ablation therapy: Effects of electrode polarization modes through in silico and in vitro studies","authors":"Enaide Maine Calzado , Nahuel Olaiz , Luis Enrique Bergues Cabrales , Pablo Turjanski","doi":"10.1016/j.bioelechem.2025.109062","DOIUrl":null,"url":null,"abstract":"<div><div>Electrolytic ablation is an emerging cancer therapy that induces tumor necrosis by applying direct electrical current through electrodes inserted locally into the tissue. The extreme cathodic and anodic pH fronts induced by the electrolytic ablation are the main cause of tumor necrosis. This study investigates how different electrode polarization modes influence the distribution of extreme pH areas, ionic species concentrations, tissue permeabilization, and overall efficacy of Electrolytic ablation therapy. Using <em>in silico</em> modeling, which solves the Nernst-Planck equations, and <em>in vitro</em> experiments on potato tubers (<em>Solanum tuberosum</em> L.) as a biological surrogate, two electrode configurations are analyzed: Configuration Ia (one anode and four cathodes) and Configuration Ib (four anodes and one cathode).</div><div>The results demonstrate that Configuration Ib produces broader extreme pH areas, larger tissue damage zones, and greater permeabilization compared to Configuration Ia. This behavior is attributed to the enhanced migration and diffusion of H<sup>+</sup> ions under the Configuration Ib polarization strategy.</div><div>These findings underscore the importance of selecting the electrode polarization mode in optimizing Electrolytic ablation therapy parameters. The broader ablation areas and higher efficacy observed with Configuration Ib suggest its potential to improve tumor treatment outcomes, particularly when combined with electroporation-based therapies.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"167 ","pages":"Article 109062"},"PeriodicalIF":4.5000,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioelectrochemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1567539425001653","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Electrolytic ablation is an emerging cancer therapy that induces tumor necrosis by applying direct electrical current through electrodes inserted locally into the tissue. The extreme cathodic and anodic pH fronts induced by the electrolytic ablation are the main cause of tumor necrosis. This study investigates how different electrode polarization modes influence the distribution of extreme pH areas, ionic species concentrations, tissue permeabilization, and overall efficacy of Electrolytic ablation therapy. Using in silico modeling, which solves the Nernst-Planck equations, and in vitro experiments on potato tubers (Solanum tuberosum L.) as a biological surrogate, two electrode configurations are analyzed: Configuration Ia (one anode and four cathodes) and Configuration Ib (four anodes and one cathode).

The results demonstrate that Configuration Ib produces broader extreme pH areas, larger tissue damage zones, and greater permeabilization compared to Configuration Ia. This behavior is attributed to the enhanced migration and diffusion of H⁺ ions under the Configuration Ib polarization strategy.

These findings underscore the importance of selecting the electrode polarization mode in optimizing Electrolytic ablation therapy parameters. The broader ablation areas and higher efficacy observed with Configuration Ib suggest its potential to improve tumor treatment outcomes, particularly when combined with electroporation-based therapies.

查看原文本刊更多论文

优化电解消融治疗：通过硅和体外研究电极极化模式的影响

电解消融是一种新兴的癌症治疗方法，通过局部插入组织的电极施加直流电诱导肿瘤坏死。电解消融引起的极端阴极和阳极pH锋是肿瘤坏死的主要原因。本研究探讨了不同电极极化模式对电解消融治疗的极端pH区分布、离子浓度、组织通透性和总体疗效的影响。采用求解Nernst-Planck方程的硅模拟方法，结合马铃薯块茎（Solanum tuberosum L.）作为生物替代品的体外实验，分析了两种电极配置：配置Ia（1阳极4阴极）和配置Ib（4阳极1阴极）。结果表明，与配置Ia相比，配置Ib产生更宽的极端pH区，更大的组织损伤区和更大的渗透性。这种行为归因于构型Ib极化策略下H+离子的迁移和扩散增强。这些发现强调了选择电极极化模式在优化电解消融治疗参数中的重要性。配置Ib观察到更广泛的消融区域和更高的疗效，这表明它有可能改善肿瘤治疗结果，特别是当与基于电穿孔的治疗联合使用时。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.