Acoustic streaming on antibody-functionalized screen-printed electrode enhances detection sensitivity and total assay duration for voltammetric immunosensing of newcastle disease virus

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2025-07-07 DOI:10.1016/j.bioelechem.2025.109043

Mohamad Farid Abd Muain , Amir Syahir Amir Hamzah , Suet Lin Chia , Khatijah Yusoff , Hong Ngee Lim , Shinya Ikeno , Thomas Laurell , Asilah Ahmad Tajudin

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引用次数: 0

Abstract

Conventional diagnostic methods often involve long incubation times due to limited fluid mixing in confined spaces, despite offering high sensitivity. Therefore, acoustic streaming was employed to enhance microscale advection, thereby improving biomolecular interactions and reducing assay duration. The micromixing capability was demonstrated by dispersing methylene blue (MB) in deionized water and glycerol solutions, where homogenization time decreased by approximately 80 % in water and 84–88 % in glycerol under acoustic actuation. Biomolecule adsorption was modeled using MB adsorbed onto cellulose acetate–graphene oxide (CA-GO) beads, showing improved adsorption and a reduced time to saturation from 16 to 8 min. Maximum adsorption occurred at 2 MHz frequency and 20 V amplitude. By using these optimized parameters, voltammetric immunosensing of Newcastle disease virus (NDV) was performed on PEG-alkanethiol-modified screen-printed gold electrodes (SPGE). The system incorporating acoustic streaming was compared against one without it. Results demonstrated a comparable limit of detection (1.46 HA μL⁻¹ at 3σ m⁻¹) achieved at shorter assay duration (8 min). These findings underscore the potential of acoustic streaming in electrochemical immunosensors to accelerate diagnostic assays without compromising sensitivity or specificity, particularly for applications utilizing screen-printed electrodes.

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在抗体功能化的丝网印刷电极上的声流增强了新城疫病毒伏安免疫传感的检测灵敏度和总检测时间

传统的诊断方法尽管具有很高的灵敏度，但由于在密闭空间中流体混合有限，通常需要较长的潜伏期。因此，声学流被用来增强微尺度平流，从而改善生物分子相互作用，缩短测定时间。通过在去离子水和甘油溶液中分散亚甲基蓝（MB），证明了微混合能力，在声驱动下，水的均质时间减少了约80%，甘油的均质时间减少了84 - 88%。将MB吸附在醋酸纤维素-氧化石墨烯（CA-GO）微珠上，模拟生物分子吸附，结果表明，MB的吸附性能得到改善，饱和时间从16分钟缩短至8分钟。在2 MHz频率和20 V振幅下，吸附量最大。利用优化后的参数，在聚乙二醇-烷硫醇修饰的丝网印刷金电极（SPGE）上对新城疫病毒（NDV）进行了伏安免疫检测。将合并声流的系统与不合并声流的系统进行了比较。结果表明，在较短的检测时间（8 min）下，检测限为1.46 HA μL−1 （3σ m−1）。这些发现强调了声流在电化学免疫传感器中的潜力，可以在不影响灵敏度或特异性的情况下加速诊断分析，特别是在使用丝网印刷电极的应用中。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.