Biomaterials Science最新文献

{"title":"Development of a bioactive hyaluronic acid hydrogel functionalised with antimicrobial peptides for the treatment of chronic wounds.","authors":"Noémie Petit, Ana Gomes, Yu-Yin Joanne Chang, Jessica Da Silva, Ermelindo C Leal, Eugénia Carvalho, Paula Gomes, Shane Browne","doi":"10.1039/d5bm00567a","DOIUrl":"https://doi.org/10.1039/d5bm00567a","url":null,"abstract":"<p><p>Chronic wounds present significant clinical challenges due to delayed healing and high infection risk. This study presents the development and characterisation of acrylated hyaluronic acid (AcHyA) hydrogels functionalised with gelatin (G) and the antimicrobial peptide (AMP) PP4-3.1 to enhance cellular responses while providing antimicrobial activity. AcHyA-G and AcHyA-AMP hydrogels were formed <i>via</i> thiol-acrylate crosslinking, enabling <i>in situ</i> AcHyA hydrogel formation with stable mechanical properties across varying gelatin concentrations. Biophysical characterisation of AcHyA-G hydrogels showed rapid gelation, elastic behaviour, uniform mesh size, and consistent molecular diffusion across all formulations. Moreover, the presence of gelatin enhanced stability without affecting the hydrogel's degradation kinetics. AcHyA-G hydrogels supported the adhesion and spreading of key cell types involved in wound repair (dermal fibroblasts and endothelial cells), with 0.5% gelatin identified as the optimal effective concentration. Furthermore, the conjugation of the AMP conferred bactericidal activity against <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>, two of the most prevalent bacterial species found in chronically infected wounds. These results highlight the dual function of AcHyA-AMP hydrogels in promoting cellular responses and antimicrobial activity, offering a promising strategy for chronic wound treatment. Further <i>in vivo</i> studies are needed to evaluate their efficacy, including in diabetic foot ulcers.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Theragenerative injectable bone-adhesive hydrogels for combined photothermal osteosarcoma therapy and bone repair.","authors":"Shiyi Chen, Nourhan Hassan, Alexander Kopp, Tatiane Eufrásio-da-Silva, Jihene Arfaoui, Benedetta Isella, Ziyaad Aytuna, Philipp Barnowski, Gerhard Sengle, Alireza Dolatshahi-Pirouz, Nadja Kröger, Hajar Homa Maleki","doi":"10.1039/d5bm00559k","DOIUrl":"https://doi.org/10.1039/d5bm00559k","url":null,"abstract":"<p><p>Injectable hydrogels with self-healing properties, tissue adhesion, biocompatibility, and cancer therapeutic capabilities offer a promising solution for addressing bone loss and residual tumor cells following surgical resection of osteosarcoma. In this study, injectable adhesive hybrid hydrogels were developed using natural silk-derived proteins, silk fibroin (SF), and silk sericin (SS). The sericin was surface functionalized with dopamine (DOPA) forming SSDOPA, while the silk fibroin was enzymatically oxidized (forming SFO) to introduce abundant catechol moieties on the polymer chains. These modifications enabled hydrogelation and self-assembly in the presence of copper ions (Cu²⁺) and tannic acid (TA), creating an SFO-SSDopa-Cu²⁺-TA hydrogel inspired by the mussel adhesion mechanism. The dynamic metal-catechol coordination bonds, along with other covalent and non-covalent interactions in the gel network, imparted excellent shear-thinning properties with 3D printability, injectability, self-healing (72.27 ± 9.35% after 6 cyclic), making it suitable for minimally invasive surgeries and targeted delivery applications. Additionally, the developed adhesive hydrogel demonstrated strong adhesiveness (664.03 ± 15.87 kPa and 854.15 ± 12.90 kPa on Gel- and Hap-based substrates respectively), showing excellent bonding performance to natural bone and tissue. Its black coloration enabled efficient absorption of near-infrared (NIR) light (reach 45-48 °C), facilitating the eradication of almost 60% osteosarcoma cells through photothermal therapy within 20 minutes of hydrogel irradiation with laser. Moreover, the developed SFO-SSDopa-Cu²⁺-TA hydrogels promoted the proliferation and migration of pre-osteoblast cells, confirming their excellent biocompatibility. Coupled with good biodegradability, these hydrogels demonstrate significant potential as theragenerative materials for minimally invasive osteosarcoma treatment, providing a clinically translatable solution for repairing bone affected by the disease.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating green solvents for sustainable PLGA nanoparticle synthesis†","authors":"Senjuti Karmaker, Rhea Joshi, Amartya Viravalli and Natalie Boehnke","doi":"10.1039/D5BM00374A","DOIUrl":"10.1039/D5BM00374A","url":null,"abstract":"<p >We report the synthesis of poly(lactic-<em>co</em>-glycolic acid) (PLGA) nanoparticles (NPs) using sustainable green solvents as safer alternatives to traditional petroleum-based, hazardous solvents. The resulting NPs are stable and exhibit comparable physicochemical properties and cellular uptake to those synthesized with traditional solvents, highlighting the potential of green solvents for NP synthesis.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 11","pages":" 2883-2890"},"PeriodicalIF":5.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR-based genetically modified scaffold-free biomaterials for tissue engineering and regenerative medicine","authors":"Yunxuan Chen, Ke Yu, Zhiwei Jiang and Guoli Yang","doi":"10.1039/D5BM00194C","DOIUrl":"10.1039/D5BM00194C","url":null,"abstract":"<p >CRISPR-based genetically modified scaffold-free biomaterials, including extracellular vehicles, cell sheets, cell aggregates, organoids and organs, have attracted significant attention in the fields of regenerative medicine and tissue engineering in recent years. With a wide range of applications in gene therapy, modeling disease, tissue regeneration, organ xenotransplantation, modeling organogenesis as well as gene and drug screening, they are at a critical juncture from clinical trials to therapeutic applications. Xenografts have already been tested on non-human primates and humans. However, we have to admit that a series of obstacles still need to be addressed, such as immune response, viral infection, off-target effects, difficulty in mass production, and ethical issues. Therefore, future research should pay more attention to improving their safety, accuracy of gene editing, flexibility of production, and ethical rationality. This review summarizes various types of CRISPR-based genetically modified scaffold-free biomaterials, including their preparation procedures, applications, and possible improvements.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 12","pages":" 3149-3175"},"PeriodicalIF":5.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogel-mediated delivery of baicalein for the effective therapy of MRSA-infected diabetic wounds by immune response and moderate photothermal effects†","authors":"Yan Wang, Suyuan Su, Chaofeng Wang, Congyang Mao, Xiangmei Liu, Hanpeng Liu, Zhaoyang Li, Shengli Zhu, Hui Jiang, Zhenduo Cui, Yufeng Zheng and Shuilin Wu","doi":"10.1039/D5BM00335K","DOIUrl":"10.1039/D5BM00335K","url":null,"abstract":"<p >Persistent bacterial infections and the imbalance in immune regulation induced by oxidative stress present a significant challenge in diabetic wound healing. In this study, we developed a novel dual-network hydrogel system composed of chitosan/polyacrylic acid (PEC) and polyoxometalate PMo<small>₁₂</small> (PMo<small>₁₂</small>-PEC) loaded with baicalein (BA), designated as PMo<small>₁₂</small>-BA-PEC, for the treatment of methicillin-resistant <em>Staphylococcus aureus</em> (MRSA)-infected diabetic wounds. The hydrogel demonstrated enhanced mechanical strength and elasticity, facilitating effective wound adherence and accommodating tissue movement. Upon 808 nm near-infrared (NIR) light irradiation, the photothermal properties of PMo<small>₁₂</small> enabled controlled low-temperature hyperthermia and accelerated BA release. Remarkably, the hydrogel achieved an antibacterial efficacy of 99.45% ± 0.12% against MRSA following 5 minutes of NIR exposure while exhibiting potent antioxidant and anti-inflammatory capabilities to scavenge reactive oxygen species and mitigate inflammatory responses. Comprehensive evaluation revealed that the PMo<small>₁₂</small>-BA-PEC hydrogel significantly promoted angiogenesis, enhanced collagen deposition, inhibited bacterial growth, and modulated immune regulation, thereby accelerating the wound healing process in diabetic rat models. These findings suggest that the PMo<small>₁₂</small>-BA-PEC hydrogel represents a promising biomaterial platform for clinical management of diabetic wounds and potential treatment of oxidative stress-related disorders.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 12","pages":" 3395-3408"},"PeriodicalIF":5.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porphyrin-based nanoscale metal–organic framework nanocarriers entrapping platinum nanoparticles and S-nitrosoglutathione for sonodynamic therapy in hypoxic tumors†","authors":"Hongbo Wang, Benchao Zheng, Shiyi Zhai, Danning Su and Kuangda Lu","doi":"10.1039/D5BM00127G","DOIUrl":"10.1039/D5BM00127G","url":null,"abstract":"<p >Sonodynamic therapy (SDT), which employs acoustic energy to generate reactive oxygen species (ROS), has emerged as a promising strategy for tumor treatment. While ultrasound (US) offers deep tissue penetration and minimal invasiveness, the low energy conversion efficiency of sonosensitizers and the hypoxic tumor microenvironment (TME) significantly limit SDT efficacy. To overcome these challenges, we developed a nano-sonosensitizer, TBP-Hf@Pt-GSNO (Hf-Pt-G), composed of a porphyrin-based nanoscale metal–organic framework (nMOF), TBP-Hf, integrated with platinum nanoparticles (Pt NPs) and S-nitrosoglutathione (GSNO). Pt NPs within the nMOF cavities enhance ultrasound reflection and scattering, thereby improving the acoustic energy conversion efficiency of TBP and boosting SDT efficacy. In addition, Pt NPs can catalyze the conversion of endogenous hydrogen peroxide (H<small>₂</small>O<small>₂</small>) into oxygen to alleviate tumor hypoxia. US irradiation further triggers the release of nitric oxide (NO) from GSNO, amplifying the killing effect on tumor cells. Enhanced singlet oxygen (<small>¹</small>O<small>₂</small>) generation and decreased hypoxia inducible factor-1α (HIF-1α) expression were observed in tumor cells following Hf-Pt-G treatment with US irradiation. <em>In vivo</em>, significant tumor suppression was achieved in 4T1 tumor-bearing mice treated with Hf-Pt-G combined with US. This study presents a novel strategy for enhancing acoustic energy conversion while integrating hypoxia alleviation and controllable NO release, thus improving the therapeutic outcomes of SDT.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 12","pages":" 3325-3335"},"PeriodicalIF":5.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A bacterial cellulose-polydopamine based injectable hydrogel for enhanced hemostasis in acute wounds†","authors":"Kaushal R. Shakya, Niranjan Chatterjee, Santosh K. Misra and Vivek Verma","doi":"10.1039/D5BM00420A","DOIUrl":"10.1039/D5BM00420A","url":null,"abstract":"<p >An injectable hydrogel hemostat composed of bacterial cellulose (BC), polydopamine and carboxymethyl cellulose (CMC) is presented as a biocompatible alternative to generally cytotoxic commercial hemostats. In this system, polydopamine (PDA) was coated on BC fibers by <em>in situ</em> oxidative polymerization, and CMC was added to improve matrix injectability, as confirmed by rheological analysis showing shear thinning behavior. The composite was characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), and scanning electron microscopy (SEM) to assess its physical, chemical and topographical characteristics. <em>In vitro</em> blood clotting tests demonstrated favorable blood clotting activity, achieving hemostasis within three minutes of application. PDA's antioxidative properties additionally helped to scavenge reactive oxygen species (ROS). The composite was tested for its compatibility with blood and mammalian cells using the <em>in vitro</em> hemolysis assay, cell viability assay, and scratch assay. <em>In vivo</em> studies using rat tail amputation and liver puncture models exhibited effective hemostasis without significant toxicity. Histological analysis of skin tissue (H&amp;E and TNF-α staining) validated the biocompatibility of the material. Thus, the BC/PDA/CMC hydrogel is a promising candidate for rapid hemostasis and wound healing, particularly in deep and irregular wounds.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 12","pages":" 3307-3324"},"PeriodicalIF":5.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatible composite hydrogel with on-demand swelling-shrinking properties for 4D bioprinting†","authors":"Peter J. Jensen, Josh P. Graham, Trevor K. Busch, Owen Fitz, Sivani Jayanadh, Thomas E. Pashuck and Tomas Gonzalez-Fernandez","doi":"10.1039/D5BM00551E","DOIUrl":"10.1039/D5BM00551E","url":null,"abstract":"<p >Hydrogels with tunable swelling and shrinking properties are of great interest in biomedical applications, particularly in wound healing, tissue regeneration, and drug delivery. Traditional hydrogels often fail to achieve high swelling without mechanical failure. In contrast, high-swelling hydrogels can absorb large amounts of liquid, expanding their volume by 10–1000 times, due to low crosslink density and the presence of hydrophilic groups. Additionally, some high-swelling hydrogels can also shrink in response to external stimuli, making them promising candidates for applications like on-demand drug delivery and biosensing. An emerging application of high-swelling hydrogels is four-dimensional (4D) printing, where controlled swelling induces structural transformations in a 3D printed construct. However, current hydrogel systems show limited swelling capacity, restricting their ability to undergo significant shape changes. To address these limitations, we developed a high-swelling composite hydrogel, termed SwellMA, by combining gelatin methacryloyl (GelMA) and sodium polyacrylate (SPA). SwellMA exhibits a swelling capacity over 500% of its original area and can increase its original water weight by 100-fold, outperforming existing materials in 4D bioprinting. Furthermore, SwellMA constructs can cyclically swell and shrink on-demand upon changing the ionic strength of the aqueous solution. Additionally, SwellMA demonstrates superior cytocompatibility and cell culture properties than SPA, along with enhanced 3D printing fidelity. These findings demonstrate SwellMA's potential for advanced 4D printing and a broad range of biomedical applications requiring precise and dynamic control over hydrogel swelling and shrinking.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 12","pages":" 3264-3279"},"PeriodicalIF":5.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d5bm00551e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overcoming biological inertness: multifaceted strategies to optimize PEEK bioactivity for interdisciplinary clinical applications","authors":"Yingjia Sun, Shixing Ma, Yang Shi, Mumian Chen, Yanhua Lan, Lingling Hu and Xiaofeng Yang","doi":"10.1039/D4BM01693A","DOIUrl":"10.1039/D4BM01693A","url":null,"abstract":"<p >Polyether ether ketone (PEEK), characterized by a comparable elastic modulus to human bone with high wear resistance, radiolucency, and biocompatibility, demonstrates considerable promise for clinical applications. However, due to the significant limitations in clinical applications caused by the biological inertness of PEEK, it should first be modified to meet clinical needs. Currently, the field of PEEK modifications is rapidly advancing, with a particular emphasis on enhancing its biological properties. Most of the previous reviews have separately discussed strategies like antibacterial, osteogenic, and angiogenic enhancements for PEEK. This review combines cross-domain insights to update and synthesize recent research on PEEK composites, focusing on advanced multi-component sustained release platforms that mimic postoperative biological processes. Such temporal alignment between material functionality and physiological healing phases demonstrates unprecedented potential for expanding PEEK's clinical versatility.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 12","pages":" 3106-3122"},"PeriodicalIF":5.8,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of MgO-loaded PLA/dECM antibacterial nanofibrous membranes for enhanced gingival regeneration†","authors":"Shu Sun, Jing Qin, Yifu Zhuang, Pengfei Cai, Xiao Yu, Hongsheng Wang, Xiumei Mo, Jinglei Wu, Mohamed EL-Newehy, Meera Moydeen Abdulhameed, Mingyue Fan, Wenhao Qian and Binbin Sun","doi":"10.1039/D4BM01346H","DOIUrl":"10.1039/D4BM01346H","url":null,"abstract":"<p >Clinically, gingival tissue repair is challenging due to the complex oral microbial environment and inflammation. The development of gingival membranes using tissue engineering techniques offers a promising solution to this issue. This study focuses on developing a nanofibrous gingival membrane, combining polylactic acid (PLA), decellularized extracellular matrix (dECM), and magnesium oxide (MgO) nanoparticles. Electrospinning was used to fabricate membranes with varying ratios of PLA, dECM, and MgO, and their mechanical, antibacterial, and cell-proliferation properties were evaluated. NIH-3T3 and rat gingival fibroblast (RGF) cells were cultured on the membranes to assess biocompatibility. A rat model with gingival defects was used to test <em>in vivo</em> tissue regeneration. It was indicated that the antibacterial nanofibrous membranes with MgO showed enhanced antibacterial effects and reduced inflammation, and promoted gingival tissue repair.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 12","pages":" 3354-3366"},"PeriodicalIF":5.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}