Biomaterials Science最新文献_第5页

Hydrogels and nanogels based on 2Nap-capped cationic tripeptides: influence of lysine position and siRNA delivery properties. 基于2nap阳离子三肽的水凝胶和纳米凝胶：赖氨酸位置和siRNA传递特性的影响。

IF 6.1 3区医学

Biomaterials Science Pub Date : 2026-09-29 DOI: 10.1039/d6bm00791k

Mariangela Rosa, Fin Hallam Stewart, Carlo Diaferia, Tomás S Plivelic, Mariano Stornaiuolo, Dave J Adams, Antonella Accardo

{"title":"Hydrogels and nanogels based on 2Nap-capped cationic tripeptides: influence of lysine position and siRNA delivery properties.","authors":"Mariangela Rosa, Fin Hallam Stewart, Carlo Diaferia, Tomás S Plivelic, Mariano Stornaiuolo, Dave J Adams, Antonella Accardo","doi":"10.1039/d6bm00791k","DOIUrl":"10.1039/d6bm00791k","url":null,"abstract":"The delivery of therapeutic short interfering RNA (siRNA) is hindered by biological barriers such as rapid degradation and poor cellular uptake. This study investigates peptide-based nanogels (NGs) as potential delivery platforms using three N-capped tripeptides, 2NapKFF, 2NapFKF, and 2NapFFK, designed to load siRNA via electrostatic interactions. The NGs were formulated through a \"top-down approach\" from pH-triggered hydrogels (HGs) and stabilized by surfactants. Biophysical characterization revealed that while all tripeptides achieved ∼99% siRNA encapsulation, the position of the lysine residue significantly influenced the stability and mechanical properties of the network. The 2NapFKF system emerged as the most suitable candidate, maintaining a size (∼200 nm) compatible with parenteral administration. Biological assays on Human Embryonic Kidney (HEK293) cells confirmed biocompatibility and cytoplasmic internalization through endocytic pathways. Furthermore, the treated cells maintained normal mitotic activity, indicating no impairment of cell proliferation. These findings demonstrate that lysine-modified tripeptide NGs are safe and effective tools for gene-silencing applications.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":"5235-5245"},"PeriodicalIF":6.1,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148785518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial metabolism-activated turn-on fluorescent liposomal probe for diagnosing bacteria-associated tumors. 细菌代谢激活的荧光脂质体探针用于诊断细菌相关肿瘤。

IF 6.1 3区医学

Biomaterials Science Pub Date : 2026-09-29 DOI: 10.1039/d6bm00968a

Siyu Zhang, Da-Yuan Wang, Xiaohan Tian, Qinghao Huo, Shuoshuo Song, Peishi Jiang, Jiafei Liu, Feihe Ma, Yi Sun, Linqi Shi

{"title":"Bacterial metabolism-activated turn-on fluorescent liposomal probe for diagnosing bacteria-associated tumors.","authors":"Siyu Zhang, Da-Yuan Wang, Xiaohan Tian, Qinghao Huo, Shuoshuo Song, Peishi Jiang, Jiafei Liu, Feihe Ma, Yi Sun, Linqi Shi","doi":"10.1039/d6bm00968a","DOIUrl":"10.1039/d6bm00968a","url":null,"abstract":"Tumor-resident bacteria have emerged as key regulators of tumor progression, metastasis, and therapeutic resistance, yet their detection remains challenging because of their low abundance and predominantly intracellular localization. Here, we report a bacterial metabolism-activated fluorescent liposomal probe for the selective detection of intratumoral bacteria. The probe was constructed by covalently conjugating hydrophilic D-alanine, a bacterial metabolizable substrate, to a hydrophobic fluorophore, yielding an amphiphilic cationic metabolic-labeling molecule, 3-HFD. In aqueous media, 3-HFD self-assembled into fluorescence-quenched nanoaggregates through aggregation-caused quenching, thereby minimizing background fluorescence. These nanoaggregates were encapsulated into charge-reversible, membrane-fusogenic DCPA-H2O liposomes to promote tumor accumulation and intracellular delivery. In the weakly acidic tumor microenvironment, DCPA-H2O underwent protonation, enhancing cellular internalization and membrane fusion. Following intracellular release, the cationic probe bound with negatively charged bacteria and was metabolically incorporated into the bacterial cell wall during peptidoglycan biosynthesis, resulting in aggregate disassembly and fluorescence recovery. This metabolism-triggered fluorescence turn-on strategy enabled selective visualization of intratumoral bacteria while minimizing false-positive signals from unactivated probes. In both bacteria-associated tumor-bearing mouse models and clinical tumor specimens, the liposomal probe detected all bacteria-positive samples identified by the gold standard, demonstrating its potential for the diagnosis of bacteria-associated tumors.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":"5218-5234"},"PeriodicalIF":6.1,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148785452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards therapeutic applications of nanobody-Fc constructs: comparative analysis of Fc receptor binding and macrophage activation. 纳米体-Fc结构的治疗应用：Fc受体结合和巨噬细胞激活的比较分析。

IF 6.1 3区医学

Biomaterials Science Pub Date : 2026-09-29 DOI: 10.1039/d6bm00509h

Miglė Stančiauskaitė, Mantvydas Usvaltas, Kristina Mašalaitė, Asta Lučiūnaitė, Ieva Plikusiene

{"title":"Towards therapeutic applications of nanobody-Fc constructs: comparative analysis of Fc receptor binding and macrophage activation.","authors":"Miglė Stančiauskaitė, Mantvydas Usvaltas, Kristina Mašalaitė, Asta Lučiūnaitė, Ieva Plikusiene","doi":"10.1039/d6bm00509h","DOIUrl":"10.1039/d6bm00509h","url":null,"abstract":"Nanobodies (Nbs) are new and promising tools for a variety of applications, including their use in medicine, although their functional activity and encounter with immune cells are still not well characterized. Our goal was to study the properties of Nbs coupled with the human IgG1 antibody Fc fragment (Nb-Fc), including their interaction with antigens, Fc receptors (FcRs), and professional phagocytes: macrophages. In our research, we employed a combination of two surface-sensitive methods: spectroscopic ellipsometry (SE) and quartz crystal microbalance with dissipation (QCM-D). It enabled the quantitative investigation of interaction kinetics between human FcγRI (CD64) and immune complexes (IC) formed by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein Wuhan variant (SCoV2-S) and specific Nb-Fc, as well as the optical and mechanical properties of the formed layers. Human THP-1 macrophage cell culture was used to analyze antigen and IC induced inflammatory response, antigen presentation-related processes, as well as chemokine CCL2/CCR2 and CCL5/CCR5 axes. Our investigated Nb-Fcs had a high affinity for SCoV2-S that was comparable to that of the natural human antibody CR3022. ICs formed by Nb-Fcs also interacted with FcRs in a similar way to CR3022. One of the investigated Nb-Fcs distinguished itself by its very high affinity and formed relatively the largest ICs. Interestingly, only ICs formed by the other Nb-Fcs influenced cellular response. We found increased cell surface expression of CD83. This molecule plays a regulatory role in antigen presentation and is involved in the resolution of inflammation following infection. In addition, we did not find any increase in other investigated markers in THP-1 macrophages, including the inflammatory response. Overall, our data demonstrate that Nb-Fcs can be applied in a similar manner to conventional human antibodies, considering their properties.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":"5246-5264"},"PeriodicalIF":6.1,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148785503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polycation chain length determines the in vivo mRNA delivery efficiency of block copolymer micelles. 多阳离子链长度决定了嵌段共聚物胶束在体内mRNA的传递效率。

IF 6.1 3区医学

Biomaterials Science Pub Date : 2026-09-29 DOI: 10.1039/d6bm00820h

Yuki Nakashima, Pengwen Chen, Guanghao Hu, Keita Masuda, Sosuke Takasugi, Horacio Cabral

{"title":"Polycation chain length determines the in vivo mRNA delivery efficiency of block copolymer micelles.","authors":"Yuki Nakashima, Pengwen Chen, Guanghao Hu, Keita Masuda, Sosuke Takasugi, Horacio Cabral","doi":"10.1039/d6bm00820h","DOIUrl":"10.1039/d6bm00820h","url":null,"abstract":"Messenger RNA (mRNA)-based therapeutics hold great promise by enabling in situ expression of therapeutic proteins. The success of these therapies in vivo critically depends on the design of safe and efficient delivery systems. Polymeric micelles formed by block catiomers can encapsulate mRNA, protect it from degradation, and facilitate intracellular delivery. Polycation chain length has been proposed as a design parameter of micelles, yet how it governs stabilizing interactions to define mRNA delivery performance remains unestablished. Here, we systematically investigate this relationship using a series of poly(ethylene glycol)-poly(glycidyl-phenylalanate) (PEG-PG(Phe)) block copolymers with varying PG(Phe) chain lengths. These polymers interact with mRNA via polyion complexation and π-π stacking. Our findings revealed that the block catiomers having PG(Phe) segments with 90 units formed micelles with superior stability and enhanced endosomal escape, while micelles with shorter segments were rapidly dissociated and micelles with longer blocks showed impaired PEG shielding. Moreover, the polymer with a PG(Phe) block of 90 units achieved 2-fold higher protein expression in vivo compared to shorter or longer chain variants. These findings reveal that tuning polycation chain length with core π-π interactions is key to unlocking highly efficient mRNA delivery.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":"5265-5278"},"PeriodicalIF":6.1,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148807892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrospun PEO/gelatin nanofibers for pDNA and mRNA delivery. 用于pDNA和mRNA传递的静电纺丝PEO/明胶纳米纤维。

IF 6.1 3区医学

Biomaterials Science Pub Date : 2026-09-29 DOI: 10.1039/d6bm00687f

Berk Akgün, Cagri Ayranci, Hasan Uludağ

{"title":"Electrospun PEO/gelatin nanofibers for pDNA and mRNA delivery.","authors":"Berk Akgün, Cagri Ayranci, Hasan Uludağ","doi":"10.1039/d6bm00687f","DOIUrl":"10.1039/d6bm00687f","url":null,"abstract":"Electrospun nanofiber mats are a versatile delivery system that can be applied locally at a target site, making them attractive for localized gene delivery. Although some studies reported electrospinning of nucleic acids, polyethylene oxide (PEO) was primarily used as a co-spinnable additive rather than as a standalone carrier in previous studies. In this study, PEO and gelatin nanofiber mats were prepared via blend and coaxial electrospinning to encapsulate pDNA and mRNA polyplexes prepared with a commercial transfection reagent. Notably, mRNA complexes were directly encapsulated within electrospun nanofibers through blend electrospinning, an approach not previously reported for mRNA delivery from fiber matrices. The results showed that PEO fiber mats exhibit higher encapsulation efficiency (∼60% for pDNA), transfection performance (∼75% of the free-complex level), and maintenance of polyplex integrity than gelatin, blend, and coaxial fibers for both types of nucleic acids. Stability studies over time have shown that encapsulation within electrospun fibers significantly increased the functional lifetimes of both polyplexes compared with non-electrospun (free) polyplexes. Free pDNA polyplexes lost ∼95% of their activity within 24 h, whereas PEO fiber-encapsulated pDNA retained most of its activity, losing only ∼35% over the same period. Cytotoxicity analysis showed that all fiber mats maintained cell viability above 70% at low-to-moderate electrospinning polymer amounts. It was thus shown that PEO electrospun nanofibers can serve as efficient standalone carriers for nucleic acid delivery, with polymer selection influencing encapsulation, transfection, and stability.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":"5157-5169"},"PeriodicalIF":6.1,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148756916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reshaping the cardiac microenvironment: a colchicine-loaded nanoplatform with lesion targeting and matrix penetration for myocardial fibrosis therapy. 重塑心脏微环境：具有病变靶向和基质渗透的秋水仙碱负载纳米平台用于心肌纤维化治疗。

IF 6.1 3区医学

Biomaterials Science Pub Date : 2026-09-29 DOI: 10.1039/d6bm00441e

Shilin Zhang, Qing Yang, Yutong Liu, Wei Lu, Meng Dang, Chunrui Liu, Fen Chen, Zhaogang Teng, Jing Yao

{"title":"Reshaping the cardiac microenvironment: a colchicine-loaded nanoplatform with lesion targeting and matrix penetration for myocardial fibrosis therapy.","authors":"Shilin Zhang, Qing Yang, Yutong Liu, Wei Lu, Meng Dang, Chunrui Liu, Fen Chen, Zhaogang Teng, Jing Yao","doi":"10.1039/d6bm00441e","DOIUrl":"10.1039/d6bm00441e","url":null,"abstract":"Myocardial fibrosis (MF), a major driver of cardiovascular disease progression, is characterized by persistent inflammation, aberrant activation of cardiac fibroblasts, and excessive extracellular matrix (ECM) deposition. Current therapeutic strategies are constrained by limited efficacy, safety concerns, and insufficient drug accumulation and distribution within fibrotic lesions due to dense ECM barriers. Herein, we developed a lesion-targeting and ECM-penetrating nanoplatform (TIMP/Col@HH) by modifying a flexible HSA-HAase (HH) nanocapsule with a tissue inhibitor of metalloproteinases (TIMP) peptide, followed by colchicine (Col) loading. In this system, the TIMP peptide promotes accumulation in fibrotic myocardium through recognition of MMP2 overexpressed in fibrotic lesions, while the HH nanocapsule facilitates intralesional distribution within the fibrotic matrix, thereby improving local delivery of colchicine. The resulting nanoparticles exhibited favorable biocompatibility, enhanced cardiac accumulation, and improved distribution in fibrotic myocardial tissue. In vitro, TIMP/Col@HH suppressed pro-inflammatory macrophage activation, inhibited cardiac fibroblast activation and collagen deposition, and interrupted inflammation-driven fibrosis in a coculture model. In an isoproterenol-induced MF mouse model, TIMP/Col@HH exerted pronounced anti-inflammatory and anti-fibrotic effects and significantly improved cardiac function. Collectively, TIMP/Col@HH represents a promising nanotherapeutic strategy for myocardial fibrosis by integrating lesion targeting, enhanced matrix penetration, and colchicine-based intervention.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":"5293-5308"},"PeriodicalIF":6.1,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148823600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A double-amine ionizable lipid library enables highly efficient mRNA delivery to T cells at reduced lipid mass. 双胺电离脂质文库使mRNA高效传递到T细胞在减少脂质质量。

IF 6.1 3区医学

Biomaterials Science Pub Date : 2026-09-29 DOI: 10.1039/d6bm00568c

Vishnu Sriram, Vidya Phatangare, Joo-Youp Lee

{"title":"A double-amine ionizable lipid library enables highly efficient mRNA delivery to T cells at reduced lipid mass.","authors":"Vishnu Sriram, Vidya Phatangare, Joo-Youp Lee","doi":"10.1039/d6bm00568c","DOIUrl":"10.1039/d6bm00568c","url":null,"abstract":"mRNA-based nucleic acid therapeutics have gained significant attention and undergone extensive clinical investigation since the FDA's approval of Spikevax and Comirnaty for the treatment of SARS-CoV-2. Lipid nanoparticles (LNPs) are currently the most advanced delivery system for delivering mRNA in vivo. However, concerns persist regarding the tolerability of their repeated use due to lipid accumulation. Over time, lipids have been optimized through the introduction of enzymatically degradable ester linkers. Yet, this modification alone does not fully address lipid clearance from the body. To mitigate these concerns, we have designed a library of novel ionizable lipids that incorporates two nitrogen atoms within the lipid backbone. We hypothesize that the LNPs with these lipids can be potentially administered at lower dosages while maintaining the same payload delivery and translation efficiency. In this study, we identified five lead LNPs that exhibited remarkable transfection efficiency without cytotoxic effects in vitro. We further optimized the LNP formulations by incorporating various cholesterol analogs to fine-tune translation performance. Upon analyzing the endocytic and intracellular pathways of the lead LNPs, we found that multiple pathways were involved in delivering the payload to the cell. This set of novel lipids holds great promise for mRNA delivery and warrants further investigation.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":"5207-5217"},"PeriodicalIF":6.1,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148785502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A glucose oxidase-powered multi-responsive composite hydrogel with a cascade-amplified antibacterial response for wound dressing. 一种葡萄糖氧化酶驱动的多响应复合水凝胶，具有级联扩增的抗菌反应，用于伤口敷料。

IF 6.1 3区医学

Biomaterials Science Pub Date : 2026-09-29 DOI: 10.1039/d6bm01027j

Qingping Liang, Zhemin Liu, Changliang Zhu, Bingxin Zhao, Haijin Mou

{"title":"A glucose oxidase-powered multi-responsive composite hydrogel with a cascade-amplified antibacterial response for wound dressing.","authors":"Qingping Liang, Zhemin Liu, Changliang Zhu, Bingxin Zhao, Haijin Mou","doi":"10.1039/d6bm01027j","DOIUrl":"https://doi.org/10.1039/d6bm01027j","url":null,"abstract":"The complex microenvironment of wounds, characterized by hyperglycemia and bacterial infections, significantly impedes the healing process, thereby necessitating the development of highly effective bio-composite wound dressings. Herein, a recombinant glucose oxidase (GOD)-based cascade catalytic MIL@GOD was fabricated and encapsulated within a carbohydrate-rich dual-network hydrogel matrix composed of carboxyethyl chitosan (CECS) and oxidized sodium alginate (OSA) and mechanically reinforcing oxidized bacterial cellulose nanocrystals (OxBCNC) to construct a multi-responsive and cascade-amplified composite hydrogel. The hydrogel demonstrated excellent mechanical properties and multiple responsiveness, including cascade catalytic activity, antibacterial activity, anti-biofilm activity, and desirable biocompatibility. The evaluation of the wound healing effects of the resultant composite hydrogels revealed that these hydrogel dressings enhanced the healing process in full-thickness skin defect models. The desirable wound healing effects may be attributed to the ability of the hydrogel to consume glucose at the wound site and self-supply H2O2 for ROS production, thereby reducing glucose concentrations while concurrently decreasing bacterial infections and inflammatory responses. Additionally, the composite hydrogel functions as a supportive dressing to facilitate collagen deposition and angiogenesis, thereby enhancing the wound healing process. These results collectively indicate that the composite hydrogel successfully constructs a GOD-mediated multi-responsive cascade platform as an antibacterial wound dressing, demonstrating significant potential for the treatment of infected wounds.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149072917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dendrimers in precision oncology: advances in therapy, diagnostics, and translational prospects. 精准肿瘤学中的树状大分子：治疗、诊断和转化前景的进展。

IF 6.1 3区医学

Biomaterials Science Pub Date : 2026-09-29 DOI: 10.1039/d6bm00242k

Davood Zaeifi, Khadijeh Jamialahmadi, Gholamreza Karimi

引用次数: 0

A compact sequence-defined repetitive peptide for topical ocular plasmid DNA delivery. 一种紧凑的序列定义的重复肽，用于局部眼部质粒DNA递送。

IF 6.1 3区医学

Biomaterials Science Pub Date : 2026-09-24 DOI: 10.1039/d6bm00916f

Hasitieer Bahetibieke, Yansong Zhang, Xia Zhang, Jiawen Ye, Lu Wang, Sicheng Tang, Xingjie Zan, Hao Chen

引用次数: 0