Biomaterials Science最新文献_第5页

Biofunctionalization of electrospun silk scaffolds with perlecan for vascular tissue engineering. 电纺丝支架血管组织工程生物功能化研究。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-05-19 DOI: 10.1039/d5bm00364d

Shouyuan Jiang, Anyu Zhang, Behnam Akhavan, John Whitelock, Marcela M Bilek, Steven G Wise, Megan S Lord, Jelena Rnjak-Kovacina

{"title":"Biofunctionalization of electrospun silk scaffolds with perlecan for vascular tissue engineering.","authors":"Shouyuan Jiang, Anyu Zhang, Behnam Akhavan, John Whitelock, Marcela M Bilek, Steven G Wise, Megan S Lord, Jelena Rnjak-Kovacina","doi":"10.1039/d5bm00364d","DOIUrl":"https://doi.org/10.1039/d5bm00364d","url":null,"abstract":"Electrospun silk fibroin scaffolds have garnered significant interest in vascular tissue engineering due to their biocompatibility, mechanical strength, and tunable degradation. However, their lack of intrinsic cell-binding domains limits endothelialization, a critical factor for vascular graft success. This study explores the biofunctionalization of electrospun silk scaffolds with recombinant perlecan domain V (rDV) using plasma immersion ion implantation (PIII) treatment, a surface modification method enabling robust covalent immobilization without the use of reagents. The biofunctionalized scaffolds enhanced endothelial cell adhesion, proliferation, and retention under physiological flow conditions while inhibiting smooth muscle cell proliferation. Additionally, the functionalized scaffolds demonstrated angiogenic potential in vivo. These findings underscore the potential of rDV-functionalized silk scaffolds as a promising candidate for small-diameter vascular grafts, addressing key challenges of endothelialization and vascular cell modulation in clinical applications.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro senescence and senolytic functional assays. 体外衰老及抗衰老功能测定。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-05-16 DOI: 10.1039/d4bm01684j

Patrick Ryan, Jungwoo Lee

{"title":"In vitro senescence and senolytic functional assays.","authors":"Patrick Ryan, Jungwoo Lee","doi":"10.1039/d4bm01684j","DOIUrl":"https://doi.org/10.1039/d4bm01684j","url":null,"abstract":"A detailed understanding of aging biology and the development of anti-aging therapeutic strategies remain imperative yet inherently challenging due to the protracted nature of aging. Cellular senescence arises naturally through replicative exhaustion and is accelerated by clinical treatments or environmental stressors. The accumulation of senescent cells-defined by a loss of mitogenic potential, resistance to apoptosis, and acquisition of a pro-inflammatory secretory phenotype-has been implicated as a key driver of chronic disease, tissue degeneration, and organismal aging. Recent studies have highlighted the therapeutic promise of senolytic drugs, which selectively eliminate senescent cells. Compelling results from preclinical animal studies and ongoing clinical trials underscore this potential. However, the clinical translation of senolytics requires further pharmacological validation to refine selectivity, minimize toxicity, and determine optimal dosing. Equally important is the evaluation of senolytics' potential to restore tissue structure and function by reducing the senescent cell burden. In vitro tissue culture models offer a powerful platform to advance these efforts. This review summarizes the current landscape of in vitro systems used for inducing cellular senescence-referred to as \"senescence assays\"-and for screening senolytic drugs-referred to as \"senolytic assays\". We conclude by discussing key challenges to improving mechanistic insight, predictive accuracy, and clinical relevance in senolytic drug development, as well as emerging applications of senolytic therapies.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From saccharides to synthetics: exploring biomaterial scaffolds as cell transduction enhancers. 从糖类到合成物：探索作为细胞转导增强剂的生物材料支架。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-05-16 DOI: 10.1039/d4bm01588f

Micah Mallory, Emma Grace Johnson, Soumen Saha, Sanika Pandit, Joshua T McCune, Mengnan Dennis, Jessica M Gluck, Craig L Duvall, Ashley C Brown, Ashutosh Chilkoti, Yevgeny Brudno

{"title":"From saccharides to synthetics: exploring biomaterial scaffolds as cell transduction enhancers.","authors":"Micah Mallory, Emma Grace Johnson, Soumen Saha, Sanika Pandit, Joshua T McCune, Mengnan Dennis, Jessica M Gluck, Craig L Duvall, Ashley C Brown, Ashutosh Chilkoti, Yevgeny Brudno","doi":"10.1039/d4bm01588f","DOIUrl":"10.1039/d4bm01588f","url":null,"abstract":"Dry, transduction biomaterial scaffolds (Drydux) represent a novel platform for enhancing viral transduction, achieving drastic improvements in transduction efficiency (from ∼10% to >80%) while simplifying production of potent genetically engineered cells. This technology addresses a critical bottleneck in cell therapy manufacturing, where conventional methods require complex protocols and often yield suboptimal results. However, the underlying material science driving Drydux-enhanced transduction remains unclear. Here, we comprehensively assess biomaterial properties that influence viral transduction enhancement through systematic testing of polysaccharides, proteins, elastin-like polypeptides (ELPs), and synthetic polymers. Our findings reveal that surface porosity and liquid absorption are primary drivers of transduction enhancement, while polymer charge and flexibility play secondary roles. Negatively charged and flexible materials-particularly gelatin, hyaluronan, and alginate-demonstrated superior performance. Notably, despite promising material characteristics, synthetic polymers failed to enhance transduction, highlighting the unique advantages of specific biomaterial compositions. By elucidating these structure-function relationships, this work establishes design principles for optimizing biomaterial-enhanced transduction and expands the Drydux platform's potential for transforming cell therapy manufacturing, regenerative medicine, and beyond.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082391/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in oxygenation nanozymes for overcoming diabetic ulcers. 氧合纳米酶治疗糖尿病溃疡的研究进展。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-05-15 DOI: 10.1039/d5bm00340g

Sumi Choi, Minjeong Kim, Minjin Kim, Su-Hwan Kim

{"title":"Advances in oxygenation nanozymes for overcoming diabetic ulcers.","authors":"Sumi Choi, Minjeong Kim, Minjin Kim, Su-Hwan Kim","doi":"10.1039/d5bm00340g","DOIUrl":"https://doi.org/10.1039/d5bm00340g","url":null,"abstract":"Diabetic ulcers, affecting 15-25% of diabetes patients worldwide, are characterized by localized hypoxia that impedes healing. This review explores the emerging field of in situ oxygen-generating nanozymes as a promising approach to diabetic ulcer treatment. Nanozymes, synthetic nanoparticles mimicking natural enzyme activities, have shown potential in generating oxygen in situ, scavenging reactive oxygen species, and modulating the wound microenvironment. Materials such as manganese dioxide, cerium dioxide, platinum nanoparticles, and molybdenum-based quantum dots have demonstrated efficacy in preclinical studies, often exhibiting multiple enzyme-like activities. These nanozymes have shown accelerated wound closure, enhanced angiogenesis, and improved tissue regeneration in animal models. However, challenges remain, including optimizing nanozyme-hydrogel interactions, addressing the potential toxicity of metal-based nanomaterials, and determining optimal oxygen concentrations for various wound conditions. Future research directions include developing biocompatible nanozymes, enhancing delivery systems, and exploring combination therapies. This review underscores the potential of nanozyme-based therapies to revolutionize the treatment of diabetic ulcers and potentially other hypoxia-related conditions.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial 3D printed gelatin scaffolds for root canal disinfection in regenerative endodontics procedures. 抗菌3D打印明胶支架根管消毒再生牙髓治疗程序。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-05-15 DOI: 10.1039/d5bm00440c

Mateo Dallos Ortega, Jenny Aveyard, Raghda Magdy Abdelgawad, Reem El-Gendy, Alexander Ciupa, David Whetnall, Julia Behnsen, Robert J Poole, Raechelle A D'Sa

{"title":"Antimicrobial 3D printed gelatin scaffolds for root canal disinfection in regenerative endodontics procedures.","authors":"Mateo Dallos Ortega, Jenny Aveyard, Raghda Magdy Abdelgawad, Reem El-Gendy, Alexander Ciupa, David Whetnall, Julia Behnsen, Robert J Poole, Raechelle A D'Sa","doi":"10.1039/d5bm00440c","DOIUrl":"https://doi.org/10.1039/d5bm00440c","url":null,"abstract":"Regenerative endodontic procedures (REPs) which aim to promote root development and pulp tissue regeneration in necrotic immature teeth, have emerged as a promising therapeutic approach. A critical determinant of REP success hinges on effective disinfection of the root canal system, which must eliminate microbial contaminants whilst preserving the microenvironment necessary for dental pulp stem cell tissue regeneration. This study reports on the fabrication of biocompatible 3D printed hydrogel scaffolds designed for root canal disinfection. The scaffolds incorporate benzyldimethyldodecylammonium chloride (BDMDAC) a broad-spectrum quaternary ammonium compound characterised by low cytotoxicity and minimal risk of resistance development. BDMDAC loaded gelatin biomaterial inks were systematically evaluated for rheology properties, mechanical stability and drug release properties. Scaffolds containing 150 μg mL-1 and 250 μg mL-1 BDMDAC exhibited excellent antimicrobial efficacy against 5 bacterial pathogens (including 3 endodontic bacteria-Enterococcus faecalis, Porphyromonas gingivalis, and Streptococcus mutans). Cytocompatibility assays using primary human dental pulp stem cells (HDPSCs) derived from 3 donors confirmed over 70% of cell viability. Furthermore, freeze-dried scaffolds demonstrated excellent shelf-life stability for at least six months. Overall, these findings highlight the potential of 3D printed BDMDAC-loaded 3D printed gelatin scaffolds as an effective and cytocompatible platform for root canal disinfection in REPs.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functionalization of viscoelastic gels with decellularized extracellular matrix microparticles enhances tissue adhesion, cell spreading, and tissue regeneration. 功能化的粘弹性凝胶与脱细胞细胞外基质微颗粒增强组织粘附，细胞扩散和组织再生。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-05-14 DOI: 10.1039/d5bm00394f

Debabrata Palai, Hana Yasue, Shima Ito, Hiyori Komatsu, Tetsushi Taguchi, Akihiro Nishiguchi

引用次数: 0

Correction: Dual bioresponsive antibiotic and quorum sensing inhibitor combination nanoparticles for treatment of Pseudomonas aeruginosa biofilms in vitro and ex vivo 校正：双生物反应性抗生素和群体感应抑制剂组合纳米颗粒用于铜绿假单胞菌生物膜的体外和体外治疗。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-05-12 DOI: 10.1039/D5BM90038G

Nishant Singh, Manuel Romero, Alessandra Travanut, Patricia F. Monteiro, Elena Jordana-Lluch, Kim R. Hardie, Paul Williams, Morgan R. Alexander and Cameron Alexander

引用次数: 0

Development of a nano-vaccine for high-grade serous ovarian cancer† 高级别浆液性卵巢癌纳米疫苗的研制

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-05-08 DOI: 10.1039/D4BM01696C

Chayanika Saha, Ahmed Elkashif, Elaine J. Gilmore, Binyumeng Jiang, Ying Sun, Raj Kumar Duary, Niamh Buckley, Nicholas J. Dunne and Helen O. McCarthy

{"title":"Development of a nano-vaccine for high-grade serous ovarian cancer†","authors":"Chayanika Saha, Ahmed Elkashif, Elaine J. Gilmore, Binyumeng Jiang, Ying Sun, Raj Kumar Duary, Niamh Buckley, Nicholas J. Dunne and Helen O. McCarthy","doi":"10.1039/D4BM01696C","DOIUrl":"10.1039/D4BM01696C","url":null,"abstract":"High-Grade Serous Carcinoma (HGSC) is characterised by aggressive malignant tumours and poor prognosis accounting for 75% of ovarian cancer. Conventional treatments often result in relapse, with a need for innovative therapeutic approaches. This study aimed to develop and evaluate a DNA vaccine targeting the preferentially expressed antigen of melanoma, PRAME, a cancer tumour antigen (CTA) overexpressed in HGSC. PRAME demonstrated the highest differential gene expression between normal fallopian tubes and HGSC tumour tissues in a range of patient datasets. The PRAME DNA was condensed by the cationic cell-penetrating peptide RALA to form nanoparticles (NPs). These self-assembling NPs exhibited a mean hydrodynamic size <150 nm and zeta potential >10 mV at N : P ratios ≥4 with ≤3% free DNA. The NPs successfully transfected NCTC-929 and DC 2.4 cells with PRAME overexpression, with negligible cytotoxicity. Vaccination with the NPs in vivo elevated CD4+ and CD8+ T-cell activation with increased expression of INF-γ and IL-2 cytokines. Vaccination also significantly improved survival rates in a PRAME-expressing tumour model in vivo. This study demonstrated the utility of a PRAME-targeted DNA vaccine for HGSC treatment which warrants further investigation.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 11","pages":" 2908-2924"},"PeriodicalIF":5.8,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d4bm01696c?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tunable hydrogel networks by varying secondary structures of hydrophilic peptoids provide viable 3D cell culture platforms for hMSCs† 通过改变亲水性肽的二级结构可调的水凝胶网络为hMSCs提供了可行的3D细胞培养平台。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-05-07 DOI: 10.1039/D5BM00433K

Aldaly Pineda-Hernandez, David A. Castilla-Casadiego, Logan D. Morton, Sebastian A. Giordano-Nguyen, Kathleen N. Halwachs and Adrianne M. Rosales

{"title":"Tunable hydrogel networks by varying secondary structures of hydrophilic peptoids provide viable 3D cell culture platforms for hMSCs†","authors":"Aldaly Pineda-Hernandez, David A. Castilla-Casadiego, Logan D. Morton, Sebastian A. Giordano-Nguyen, Kathleen N. Halwachs and Adrianne M. Rosales","doi":"10.1039/D5BM00433K","DOIUrl":"10.1039/D5BM00433K","url":null,"abstract":"Hydrogels have excellent ability to mimic the extracellular matrix (ECM) during 3D cell culture, yet it remains difficult to tune their mechanical properties without also changing network connectivity. Previously, we developed 2D culture platforms based on tunable hydrogels crosslinked by peptoids with various secondary structures: helical, non-helical, and unstructured, which allowed control over hydrogel mechanics independent of network connectivity. Here, we extend our strategy to 3D matrices by modifying the peptoids with piperazine and homopiperazine residues to enhance water solubility without altering their secondary structure. Hydrogels crosslinked with helical peptoids exhibited significantly higher stiffness compared to hydrogels crosslinked with non-helical or unstructured peptoids. Human mesenchymal stem cells (hMSCs) encapsulated within these hydrogels were assessed for viability, proliferation, and immunomodulatory potential. The stiffest hydrogels promoted the highest rates of proliferation and increased yes-associated protein (YAP) nuclear localization. Softer hydrogels, however, showed enhanced production of indoleamine 2,3-dioxygenase (IDO), both with and without interferon gamma (IFN-γ) stimulation, highlighting their potential in immunomodulatory applications. The biomimetic platform developed here enables the study of how matrix mechanics influence stem cell behavior without confounding factors from network connectivity, leading to insights for hMSC-mediated immunomodulation.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 12","pages":" 3380-3394"},"PeriodicalIF":5.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12068446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Urethral reconstruction using an amphiphilic tissue-engineered autologous polyurethane nanofiber scaffold with rapid vascularization function 更正：使用具有快速血管化功能的两亲性组织工程自体聚氨酯纳米纤维支架进行尿道重建。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-05-07 DOI: 10.1039/D5BM90034D

Yuqing Niu, Guochang Liu, Chuangbi Chen, Ming Fu, Wen Fu, Zhang Zhao, Huimin Xia and Florian J. Stadler

引用次数: 0