Biomaterials Science最新文献_第5页

Correction: Biomimetic proteoglycan nanoparticles for growth factor immobilization and delivery 修正：用于生长因子固定和输送的仿生蛋白多糖纳米颗粒。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-03-03 DOI: 10.1039/D5BM90019K

Nooshin Zandi, Ebrahim Mostafavi, Mohammad Ali Shokrgozar, Elnaz Tamjid, Thomas J. Webster, Nasim Annabi and Abdolreza Simchi

引用次数: 0

A Nd–Yb ratiometric luminescent nanothermometer for assessing thermal resistance discrepancies between A549 and BEAS-2B cells to achieve selective hyperthermia† 一种Nd-Yb比例发光纳米温度计，用于评估A549和BEAS-2B细胞之间的热阻差异，以实现选择性热疗。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-03-01 DOI: 10.1039/D4BM01729C

Yishuo Sun, Qingbing Wang, Na Wu, Mengya Kong, Yuyang Gu and Wei Feng

{"title":"A Nd–Yb ratiometric luminescent nanothermometer for assessing thermal resistance discrepancies between A549 and BEAS-2B cells to achieve selective hyperthermia†","authors":"Yishuo Sun, Qingbing Wang, Na Wu, Mengya Kong, Yuyang Gu and Wei Feng","doi":"10.1039/D4BM01729C","DOIUrl":"10.1039/D4BM01729C","url":null,"abstract":"Temperature is a crucial physical parameter in living organisms, directly associated with cellular activities. Elevated temperatures induce cell death, thereby establishing hyperthermia as a viable modality for cancer therapy. The demand for determining appropriate cancer types for hyperthermia lies in identifying cancer cells that exhibit poorer heat tolerance compared to normal cells. Herein, we have designed NaNdF4:4%Yb@NaYF4 with bright luminescence in the near-infrared region for the purpose of achieving in situ cellular temperature detection. The Nd–Yb nanothermometer provides temperature feedback based on a ratiometric luminescence intensity signal. By employing a universal cytobiology method to assess the heat resistance differences between cancer cells and normal cells across various organs, it has been observed that lung epithelial cells exhibit superior heat resistance compared to lung cancer cells. Once the Nd–Yb nanothermometer incubates within lung cells, the temperature differences between live and dead cells can be detected. The absolute temperature differences between live and dead lung cancer cells (0.1 °C) and lung epithelial cells (1.4 °C) under identical thermal stimulation (50 °C) are detected by the Nd–Yb co-doped nanothermometer, confirming that the heat resistance of normal lung cells is significantly superior to that of lung cancer cells. The differential heat resistance of lung cells enables selective hyperthermia for killing A549 cells while maximally protecting BEAS-2B cells. This research may establish rare earth nanothermometry as a valuable protocol for assessing cellular heat resistance, thereby guiding selective hyperthermia for precise lung cancer treatment.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 8","pages":" 2102-2114"},"PeriodicalIF":5.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143583970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An esterase-activated prodrug against pancreatic cancer by imaging-guided photodynamic immunotherapy† 一种通过成像引导光动力免疫疗法治疗胰腺癌的酯酶激活前药。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-02-28 DOI: 10.1039/D4BM01718H

Kaini Yang, Qingyang Sha, Xinsheng Li, Jianli Hua and Wei Chen

{"title":"An esterase-activated prodrug against pancreatic cancer by imaging-guided photodynamic immunotherapy†","authors":"Kaini Yang, Qingyang Sha, Xinsheng Li, Jianli Hua and Wei Chen","doi":"10.1039/D4BM01718H","DOIUrl":"10.1039/D4BM01718H","url":null,"abstract":"Photodynamic therapy (PDT) has received much attention as a promising modality for tumor treatment. However, the weak targeting ability of conventional photosensitisers and the metastasis of malignant tumors have severely limited the development of PDT. To address this, an esterase-activated prodrug (BPYM) has been developed for imaging-guided photodynamic therapy cascade immunotherapy for the treatment of pancreatic cancer. Upon reaction with esterase, BPYM releases the photosensitiser BPY and exhibits strong red fluorescence emission, which is further enhanced by the aggregation-induced emission (AIE) characteristics of BPY. Interestingly, the activation of the fluorescence signal simultaneously indicates the activation of photosensitivity capabilities. Under white light irradiation, activated BPYM can generate large amounts of reactive oxygen species (ROS) to induce apoptosis in pancreatic cancer cells. More importantly, BPYM-mediated PDT can trigger immunogenic cell death (ICD) and elicit a systemic anti-tumor immune response. Ultimately, this imaging-guided PDT not only precisely ablates the primary pancreatic cancer tumors, but also inhibits the growth of distant tumors through an immune response. In summary, we report a strategy to achieve photodynamic immunotherapy for the treatment of pancreatic cancer through the rational design of an esterase-activated prodrug.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 8","pages":" 2092-2101"},"PeriodicalIF":5.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic organ-on-a-chip models for the gut–liver axis: from structural mimicry to functional insights 肠道-肝脏轴的微流控芯片器官模型：从结构模拟到功能洞察。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-02-28 DOI: 10.1039/D4BM01273A

Wanlin Hu, Yushen Wang, Junlei Han, Wenhong Zhang, Jun Chen, Xinyu Li and Li Wang

{"title":"Microfluidic organ-on-a-chip models for the gut–liver axis: from structural mimicry to functional insights","authors":"Wanlin Hu, Yushen Wang, Junlei Han, Wenhong Zhang, Jun Chen, Xinyu Li and Li Wang","doi":"10.1039/D4BM01273A","DOIUrl":"10.1039/D4BM01273A","url":null,"abstract":"The gut–liver axis plays a crucial role in maintaining metabolic balance and overall human health. It orchestrates various processes, such as blood flow, nutrient transfer, metabolite processing, and immune cell communication between the two organs. Traditional methods, such as animal models and two-dimensional (2D) cell cultures, are insufficient in fully replicating the intricate functions of the gut–liver axis. The emergence of microfluidic technology has revolutionized this field, facilitating the development of organ-on-a-chip (OOC) systems. These systems are capable of mimicking the complex structures and dynamic environments of the gut and liver in vitro and incorporating sensors for real-time monitoring. In this article, we review the latest progress in gut-on-a-chip (GOC) and liver-on-a-chip (LOC) systems, as well as the integrated gut–liver-on-a-chip (GLOC) models. Our focus lies in the simulation of physiological parameters, three-dimensional (3D) structural mimicry, microbiome integration, and multicellular co-culture. All these aspects are essential for constructing accurate in vitro models of the gut and liver. Furthermore, we explore the current applications of OOC technology in the study of the gut and liver, including its use in disease modeling, toxicity testing, and drug screening. Finally, we discuss the challenges that remain and outline potential future directions for advancing GOC and LOC development in vitro.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 7","pages":" 1624-1656"},"PeriodicalIF":5.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Embedded bioprinting of dense cellular constructs in bone allograft-enhanced hydrogel matrices for bone tissue engineering. 用于骨组织工程的骨异体移植增强型水凝胶基质中致密细胞构建体的嵌入式生物打印。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-02-28 DOI: 10.1039/d4bm01616e

Hang Truong, Alperen Abaci, Hadis Gharacheh, Murat Guvendiren

{"title":"Embedded bioprinting of dense cellular constructs in bone allograft-enhanced hydrogel matrices for bone tissue engineering.","authors":"Hang Truong, Alperen Abaci, Hadis Gharacheh, Murat Guvendiren","doi":"10.1039/d4bm01616e","DOIUrl":"https://doi.org/10.1039/d4bm01616e","url":null,"abstract":"Bone tissue engineering aims to address critical-sized defects by developing biomimetic scaffolds that promote repair and regeneration. This study introduces a material extrusion-based embedded bioprinting approach to fabricate dense cellular constructs within methacrylated hyaluronic acid (MeHA) hydrogels enhanced with bioactive microparticles. Composite matrices containing human bone allograft or tricalcium phosphate (TCP) particles were evaluated for their rheological, mechanical, and osteoinductive properties. High cell viability (>95%) and uniform strand dimensions were achieved across all bioprinting conditions, demonstrating the method's ability to preserve cellular integrity and structural fidelity. The inclusion of bone or TCP particles did not significantly alter the viscosity, crosslinking kinetics, or compressive modulus of the MeHA hydrogels, ensuring robust mechanical stability and shape retention. However, bone allograft particles significantly enhanced osteogenic differentiation of human mesenchymal stem cells (hMSCs), as evidenced by increased alkaline phosphatase (ALP) activity and calcium deposition. Notably, osteogenesis was observed even in basal media, with a dose-dependent response to bone particle concentration, highlighting the intrinsic bioactivity of allograft particles. This study demonstrates the potential of combining embedded bioprinting with bioactive matrices to create dense, osteoinductive cellular constructs. The ability to induce osteogenesis without external growth factors positions this platform as a scalable and clinically relevant solution for bone repair and regeneration.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescent p53 helix mimetics pairing anticancer and bioimaging properties. 荧光p53螺旋模拟物配对抗癌和生物成像特性。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-02-26 DOI: 10.1039/d4bm01681e

Sintu Karmakar, Mimasha Mallik, Sushree Sulava, Unnati Modi, Suryanarayana Allu, Shruti Sangwan, Srinu Tothadi, J Prakasha Reddy, Rajesh Vasita, Ashwini K Nangia, Debasmita Pankaj Alone, Panchami Prabhakaran

{"title":"Fluorescent p53 helix mimetics pairing anticancer and bioimaging properties.","authors":"Sintu Karmakar, Mimasha Mallik, Sushree Sulava, Unnati Modi, Suryanarayana Allu, Shruti Sangwan, Srinu Tothadi, J Prakasha Reddy, Rajesh Vasita, Ashwini K Nangia, Debasmita Pankaj Alone, Panchami Prabhakaran","doi":"10.1039/d4bm01681e","DOIUrl":"https://doi.org/10.1039/d4bm01681e","url":null,"abstract":"Fluorescent therapeutic molecules offer a unique platform to study cellular uptake and biological pathways of drug candidates. Inhibition of the p53-HDM2 protein complex with the reactivation of the p53 pathway leading to apoptosis is a promising way to overcome the barriers and challenges in cancer therapeutic design. Although p53 helix mimetics based on the 'hotspots' design using either helical or non-helical backbones are known, cell-permeable and biocompatible inherently fluorescent helix mimetics have not yet been described. We report theragnostic helix mimetics featuring both therapeutic and bioimaging properties in a cancer cell model for the first time. The solvatochromic phthalimide unit in the scaffold functions as a site to append the hotspot mimicking residues, helps in the intramolecular hydrogen bonding mediated pre-organization of side chains on one face, and importantly, exhibits intrinsic fluorescence. The design of the mimetics, synthesis, conformational studies, and molecular docking results are discussed. In vitro cytotoxicity studies were carried out on four cell lines: U87MG (human glioblastoma), A549 (human non-small cell lung cancer), MDA-MB-231 (human triple-negative breast cancer) and HEK293 (non-cancerous cell line). The molecules showed anticancer activity in the micromolar range. The fluorescence properties provided valuable insights into their cellular permeability, distribution, and selectivity towards cancer cells and can shed light on their mechanisms of action.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifunctional chrysin-loaded gallic acid–glycerol monostearate conjugate-based injectable hydrogel for targeted inhibition of hypoxia-induced NLRP3 inflammasome in ulcerative colitis† 基于单硬脂酸甘油酯共轭物的多功能金丝桃素注射水凝胶，用于靶向抑制溃疡性结肠炎中缺氧诱导的 NLRP3 炎症小体。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-02-25 DOI: 10.1039/D4BM01700E

Ajay kumar, Rahul, Kanika, Jattin Kumar, Shubham Mahajan, Aneesh Ali, Nemat Ali, Mahendra Bishnoi, Young-Ok Son and Rehan Khan

{"title":"Multifunctional chrysin-loaded gallic acid–glycerol monostearate conjugate-based injectable hydrogel for targeted inhibition of hypoxia-induced NLRP3 inflammasome in ulcerative colitis†","authors":"Ajay kumar, Rahul, Kanika, Jattin Kumar, Shubham Mahajan, Aneesh Ali, Nemat Ali, Mahendra Bishnoi, Young-Ok Son and Rehan Khan","doi":"10.1039/D4BM01700E","DOIUrl":"10.1039/D4BM01700E","url":null,"abstract":"Ulcerative colitis (UC) is a chronic inflammatory condition affecting the colon part of the large intestine. Since there is no cure for this disease, conventional therapies only provide symptomatic relief. Recently, phytomolecules have shown promising treatment results in various diseases. However, short half-life, hydrophobicity, and poor bioavailability limit their therapeutic potential. To overcome all these challenges, we have earlier conjugated a phytomolecule (gallic acid) (GA) with the FDA-approved generally recognized as safe (GRAS) material that is glycerol monostearate (GMS). This GA–GMS conjugate self-assembles as a hydrogel via the heating–cooling method and acts as a pro-drug of GA. The in vivo imaging results suggest that the GA–GMS hydrogel more efficiently adheres to the inflamed colon than a therapeutic enema. Additionally, it is known that the gut microbiota exaggerates UC by creating a hypoxic environment in the colon. This hypoxia is linked with NLRP3 inflammasome activation that triggers the release of IL-1β and IL-18 that downregulates MUC2 protein expression in the colon, responsible for mucin secretion in the colon. Therefore, chrysin (CR) (HIF-1α inhibitor) is encapsulated into the GA–GMS hydrogel to target hypoxia. The CR@GA–GMS hydrogel follows the enzyme-responsive release of the CR and restores DSS-induced damage to colonic tissue. Furthermore, the CR@GA–GMS hydrogel downregulates HIF-1α mediated NLRP3 inflammasome signalling while upregulating MUC2 production.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 7","pages":" 1801-1817"},"PeriodicalIF":5.8,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactobacillus-derived artificial extracellular vesicles for skin rejuvenation and prevention of photo-aging† 乳酸杆菌衍生的人工细胞外囊泡用于皮肤年轻化和预防光老化。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-02-25 DOI: 10.1039/D4BM01644K

Seongsu Kang, Saetbyeol Jeon, Hwira Baek, Sunghwan Hwang, Seulgi Kim, Sung Hun Youn, Jin Woong Kim, Seung-Hyun Jun and Nae-Gyu Kang

引用次数: 0

A near infrared light activated phenothiazine based cancer cell specific phototherapeutic system: a synergistic approach to chemo-photothermal therapy† 基于近红外线激活吩噻嗪的癌细胞特异性光疗系统：化疗-光热疗法的协同方法。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-02-25 DOI: 10.1039/D4BM01288G

Mamata Ojha, Pragya Trivedi, Moumita Banerjee, Malabika Bera, Susmita Dey, Amit Kumar Singh, Avijit Jana and N. D. Pradeep Singh

{"title":"A near infrared light activated phenothiazine based cancer cell specific phototherapeutic system: a synergistic approach to chemo-photothermal therapy†","authors":"Mamata Ojha, Pragya Trivedi, Moumita Banerjee, Malabika Bera, Susmita Dey, Amit Kumar Singh, Avijit Jana and N. D. Pradeep Singh","doi":"10.1039/D4BM01288G","DOIUrl":"10.1039/D4BM01288G","url":null,"abstract":"In the pursuit of more effective cancer therapies, phototherapy has emerged as a promising approach due to its non-invasive nature and high precision. This study presents the development of a near-infrared (NIR) light-responsive phenothiazine (PTZ) based phototherapeutic system designed for targeted cancer treatment. This phototherapeutic system integrates four crucial elements for enhanced therapeutic efficacy: cancer cell-specific activity, mitochondrial targeting, photothermal conversion, and controlled drug release. The PTZ system utilizes the acidochromic 1,3-oxazine ring, which opens in the acidic tumor microenvironment, forming a positive iminium ion (C<img>N+). This ionic species targets cancer cell mitochondria, ensuring precise localization. Under NIR light irradiation (640 nm), the phototherapeutic system undergoes a red shift in the absorption and reduction in the fluorescence intensity, demonstrating a significant photothermal effect that converts light to heat, thereby inducing tumor cell apoptosis. Furthermore, NIR light triggers the controlled release of the anticancer drug chlorambucil, enabling precise spatiotemporal drug delivery. The closed form of the phototherapeutic system also facilitates drug release upon visible light irradiation (≥410 nm) with high photochemical efficiency. This dual-mode photothermal and photocontrolled drug delivery offers a synergistic approach to cancer therapy, maximizing therapeutic outcomes while minimizing side effects. Our findings underscore the potential of this innovative phototherapeutic system to advance cancer treatment through targeted, controlled, and effective drug delivery.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 7","pages":" 1818-1830"},"PeriodicalIF":5.8,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Control and interplay of scaffold–biomolecule interactions applied to cartilage tissue engineering 软骨组织工程中支架-生物分子相互作用的控制与相互作用。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-02-25 DOI: 10.1039/D5BM00049A

Silouane Dupuy, Jérémy Salvador, Marie Morille, Danièle Noël and Emmanuel Belamie

{"title":"Control and interplay of scaffold–biomolecule interactions applied to cartilage tissue engineering","authors":"Silouane Dupuy, Jérémy Salvador, Marie Morille, Danièle Noël and Emmanuel Belamie","doi":"10.1039/D5BM00049A","DOIUrl":"10.1039/D5BM00049A","url":null,"abstract":"Cartilage tissue engineering based on the combination of biomaterials, adult or stem cells and bioactive factors is a challenging approach for regenerative medicine with the aim of achieving the formation of a functional neotissue stable in the long term. Various 3D scaffolds have been developed to mimic the extracellular matrix environment and promote cartilage repair. In addition, bioactive factors have been extensively employed to induce and maintain the cartilage phenotype. However, the spatiotemporal control of bioactive factor release remains critical for maximizing the regenerative potential of multipotent cells, such as mesenchymal stromal cells (MSCs), and achieving efficient chondrogenesis and sustained tissue homeostasis, which are essential for the repair of hyaline cartilage. Despite advances, the effective delivery of bioactive factors is limited by challenges such as insufficient retention at the site of injury and the loss of therapeutic efficacy due to uncontrolled drug release. These limitations have prompted research on biomolecule–scaffold interactions to develop advanced delivery systems that provide sustained release and controlled bioavailability of biological factors, thereby improving therapeutic outcomes. This review focuses specifically on biomaterials (natural, hybrid and synthetic) and biomolecules (molecules, proteins, nucleic acids) of interest for cartilage engineering. Herein, we review in detail the approaches developed to maintain the biomolecules in scaffolds and control their release, based on their chemical nature and structure, through steric, non-covalent and/or covalent interactions, with a view to their application in cartilage repair.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 8","pages":" 1871-1900"},"PeriodicalIF":5.8,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d5bm00049a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0