Biomaterials Science最新文献

{"title":"Fluorinated poly(aryl ether)/polypropylene composite patch for prevention of abdominal adhesions after hernia repairs†","authors":"Wenqing Zhang, Huawei Yang, Xu Zhang, Zhaoyang Wang and Shifang Luan","doi":"10.1039/D4BM01704H","DOIUrl":"10.1039/D4BM01704H","url":null,"abstract":"<p >Hernia typically does not heal spontaneously. Large-pore patches, most notably polypropylene patches (PP patches), are the gold standard in hernia repair surgery. However, a single patch is insufficient for both anti-adhesion and tissue fusion, leading to complications such as organ adhesions. In this study, a chemically stable and biocompatible modified fluorinated poly(aryl ether) (FPAE-F) was prepared by grafting perfluoroalkyl groups onto a fluorinated poly(aryl ether) <em>via</em> nucleophilic aromatic substitution. A porous FPAE-F fiber film (eFPAE-F) was fabricated by electrospinning and combined with a PP patch to produce a modified fluorinated poly(aryl ether)/polypropylene (FPAE-F/PP) composite patch. The eFPAE-F layer of the composite patch, which faces the abdominal viscera, exhibits a water contact angle of 151.3 ± 1.2°. This superhydrophobic surface prevents protein adhesion, thereby inhibiting rapid fibroblast proliferation. The small pore size (3.22 ± 1.25 μm) of the eFPAE-F layer effectively impedes fibroblast infiltration while permitting the transport and metabolism of nutrients. <em>In vivo</em> experiments have demonstrated that the composite patch is a viable anti-adhesion material, resulting in no adhesions and low inflammation levels after 2 weeks. Due to its outstanding anti-adhesion properties, eFPAE-F/PP is expected to be applied in the field of hernia repair.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 8","pages":" 2134-2141"},"PeriodicalIF":5.8,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143622807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polycaprolactone/sodium alginate coaxial wet-spun fibers modified with carbon nanofibers and ceftazidime for improved clotting and infection control in wounds†","authors":"Elina Marinho, Beatriz M. Silva, Catarina S. Miranda, Sonia L. C. Pinho and Helena P. Felgueiras","doi":"10.1039/D4BM01667J","DOIUrl":"10.1039/D4BM01667J","url":null,"abstract":"<p >Chronic wounds (CWs) are a significant public health concern and affect 1–2% of the world's population. They are responsible for high morbidity and mortality rates. Bacterial infections caused by <em>Staphylococcus aureus</em> and <em>Pseudomonas aeruginosa</em> are very common in CWs and prevent normal wound healing steps from taking place. Carbon nanofibers (CNFs) have attracted interest due to their inherent antibacterial and blood clotting abilities, as well as mechanical strength. The aim of this research was to engineer coaxial fibers by wet-spinning as new platforms for drug delivery in CW care (promoting rapid blood clotting and consequent tissue regeneration). Coaxial fibers were produced with an outer layer (shell) made of a mechanically resilient polycaprolactone (PCL at 10 wt%) reinforced with carbon nanofibers (CNFs at 50, 100, and 150 μg mL<small>⁻¹</small>), while the inner layer (core) was made of a highly hydrated mixture of 2 wt% sodium alginate (SA) loaded with ceftazidime (CZ) at 128 μg mL<small>⁻¹</small> (minimum bactericidal concentration). The fibers’ double-layer structure was verified by scanning electron microscopy. Core–shell fibers were deemed highly flexible and mechanically resilient and resistant to rupture, with such properties being improved with the incorporation of CNFs. Most fibers preserved their structural integrity after 28 days of incubation in physiological-like medium. Furthermore, data reported the ability of CZ combined with CNFs to fight microbial proliferation and showed that the presence of CNFs promoted blood clotting, with PCL/CNFs50 being the most effective from the group. It was found that higher concentrations of CNFs had a detrimental effect, highlighting a concentration-dependent response. The presence of PLC in the fibers resulted in a mitigation of the CNFs’ cytotoxic impact on keratinocytes. The incorporation of CZ had no effect on the metabolic activity of the cells. Overall, the results demonstrated the potentialities of the engineered coaxial fibers for applications in wound care.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 8","pages":" 2047-2065"},"PeriodicalIF":5.8,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Biomimetic proteoglycan nanoparticles for growth factor immobilization and delivery","authors":"Nooshin Zandi, Ebrahim Mostafavi, Mohammad Ali Shokrgozar, Elnaz Tamjid, Thomas J. Webster, Nasim Annabi and Abdolreza Simchi","doi":"10.1039/D5BM90019K","DOIUrl":"10.1039/D5BM90019K","url":null,"abstract":"<p >Correction for ‘Biomimetic proteoglycan nanoparticles for growth factor immobilization and delivery’ by Nooshin Zandi <em>et al.</em>, <em>Biomater. Sci.</em>, 2020, <strong>8</strong>, 1127–1136, https://doi.org/10.1039/C9BM00668K.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 7","pages":" 1858-1860"},"PeriodicalIF":5.8,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11874909/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Nd–Yb ratiometric luminescent nanothermometer for assessing thermal resistance discrepancies between A549 and BEAS-2B cells to achieve selective hyperthermia†","authors":"Yishuo Sun, Qingbing Wang, Na Wu, Mengya Kong, Yuyang Gu and Wei Feng","doi":"10.1039/D4BM01729C","DOIUrl":"10.1039/D4BM01729C","url":null,"abstract":"<p >Temperature is a crucial physical parameter in living organisms, directly associated with cellular activities. Elevated temperatures induce cell death, thereby establishing hyperthermia as a viable modality for cancer therapy. The demand for determining appropriate cancer types for hyperthermia lies in identifying cancer cells that exhibit poorer heat tolerance compared to normal cells. Herein, we have designed NaNdF<small>₄</small>:4%Yb@NaYF<small>₄</small> with bright luminescence in the near-infrared region for the purpose of achieving <em>in situ</em> cellular temperature detection. The Nd–Yb nanothermometer provides temperature feedback based on a ratiometric luminescence intensity signal. By employing a universal cytobiology method to assess the heat resistance differences between cancer cells and normal cells across various organs, it has been observed that lung epithelial cells exhibit superior heat resistance compared to lung cancer cells. Once the Nd–Yb nanothermometer incubates within lung cells, the temperature differences between live and dead cells can be detected. The absolute temperature differences between live and dead lung cancer cells (0.1 °C) and lung epithelial cells (1.4 °C) under identical thermal stimulation (50 °C) are detected by the Nd–Yb co-doped nanothermometer, confirming that the heat resistance of normal lung cells is significantly superior to that of lung cancer cells. The differential heat resistance of lung cells enables selective hyperthermia for killing A549 cells while maximally protecting BEAS-2B cells. This research may establish rare earth nanothermometry as a valuable protocol for assessing cellular heat resistance, thereby guiding selective hyperthermia for precise lung cancer treatment.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 8","pages":" 2102-2114"},"PeriodicalIF":5.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143583970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An esterase-activated prodrug against pancreatic cancer by imaging-guided photodynamic immunotherapy†","authors":"Kaini Yang, Qingyang Sha, Xinsheng Li, Jianli Hua and Wei Chen","doi":"10.1039/D4BM01718H","DOIUrl":"10.1039/D4BM01718H","url":null,"abstract":"<p >Photodynamic therapy (PDT) has received much attention as a promising modality for tumor treatment. However, the weak targeting ability of conventional photosensitisers and the metastasis of malignant tumors have severely limited the development of PDT. To address this, an esterase-activated prodrug (<strong>BPYM</strong>) has been developed for imaging-guided photodynamic therapy cascade immunotherapy for the treatment of pancreatic cancer. Upon reaction with esterase, <strong>BPYM</strong> releases the photosensitiser <strong>BPY</strong> and exhibits strong red fluorescence emission, which is further enhanced by the aggregation-induced emission (AIE) characteristics of <strong>BPY</strong>. Interestingly, the activation of the fluorescence signal simultaneously indicates the activation of photosensitivity capabilities. Under white light irradiation, activated <strong>BPYM</strong> can generate large amounts of reactive oxygen species (ROS) to induce apoptosis in pancreatic cancer cells. More importantly, <strong>BPYM</strong>-mediated PDT can trigger immunogenic cell death (ICD) and elicit a systemic anti-tumor immune response. Ultimately, this imaging-guided PDT not only precisely ablates the primary pancreatic cancer tumors, but also inhibits the growth of distant tumors through an immune response. In summary, we report a strategy to achieve photodynamic immunotherapy for the treatment of pancreatic cancer through the rational design of an esterase-activated prodrug.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 8","pages":" 2092-2101"},"PeriodicalIF":5.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfluidic organ-on-a-chip models for the gut–liver axis: from structural mimicry to functional insights","authors":"Wanlin Hu, Yushen Wang, Junlei Han, Wenhong Zhang, Jun Chen, Xinyu Li and Li Wang","doi":"10.1039/D4BM01273A","DOIUrl":"10.1039/D4BM01273A","url":null,"abstract":"<p >The gut–liver axis plays a crucial role in maintaining metabolic balance and overall human health. It orchestrates various processes, such as blood flow, nutrient transfer, metabolite processing, and immune cell communication between the two organs. Traditional methods, such as animal models and two-dimensional (2D) cell cultures, are insufficient in fully replicating the intricate functions of the gut–liver axis. The emergence of microfluidic technology has revolutionized this field, facilitating the development of organ-on-a-chip (OOC) systems. These systems are capable of mimicking the complex structures and dynamic environments of the gut and liver <em>in vitro</em> and incorporating sensors for real-time monitoring. In this article, we review the latest progress in gut-on-a-chip (GOC) and liver-on-a-chip (LOC) systems, as well as the integrated gut–liver-on-a-chip (GLOC) models. Our focus lies in the simulation of physiological parameters, three-dimensional (3D) structural mimicry, microbiome integration, and multicellular co-culture. All these aspects are essential for constructing accurate <em>in vitro</em> models of the gut and liver. Furthermore, we explore the current applications of OOC technology in the study of the gut and liver, including its use in disease modeling, toxicity testing, and drug screening. Finally, we discuss the challenges that remain and outline potential future directions for advancing GOC and LOC development <em>in vitro</em>.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 7","pages":" 1624-1656"},"PeriodicalIF":5.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Embedded bioprinting of dense cellular constructs in bone allograft-enhanced hydrogel matrices for bone tissue engineering.","authors":"Hang Truong, Alperen Abaci, Hadis Gharacheh, Murat Guvendiren","doi":"10.1039/d4bm01616e","DOIUrl":"https://doi.org/10.1039/d4bm01616e","url":null,"abstract":"<p><p>Bone tissue engineering aims to address critical-sized defects by developing biomimetic scaffolds that promote repair and regeneration. This study introduces a material extrusion-based embedded bioprinting approach to fabricate dense cellular constructs within methacrylated hyaluronic acid (MeHA) hydrogels enhanced with bioactive microparticles. Composite matrices containing human bone allograft or tricalcium phosphate (TCP) particles were evaluated for their rheological, mechanical, and osteoinductive properties. High cell viability (>95%) and uniform strand dimensions were achieved across all bioprinting conditions, demonstrating the method's ability to preserve cellular integrity and structural fidelity. The inclusion of bone or TCP particles did not significantly alter the viscosity, crosslinking kinetics, or compressive modulus of the MeHA hydrogels, ensuring robust mechanical stability and shape retention. However, bone allograft particles significantly enhanced osteogenic differentiation of human mesenchymal stem cells (hMSCs), as evidenced by increased alkaline phosphatase (ALP) activity and calcium deposition. Notably, osteogenesis was observed even in basal media, with a dose-dependent response to bone particle concentration, highlighting the intrinsic bioactivity of allograft particles. This study demonstrates the potential of combining embedded bioprinting with bioactive matrices to create dense, osteoinductive cellular constructs. The ability to induce osteogenesis without external growth factors positions this platform as a scalable and clinically relevant solution for bone repair and regeneration.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescent p53 helix mimetics pairing anticancer and bioimaging properties.","authors":"Sintu Karmakar, Mimasha Mallik, Sushree Sulava, Unnati Modi, Suryanarayana Allu, Shruti Sangwan, Srinu Tothadi, J Prakasha Reddy, Rajesh Vasita, Ashwini K Nangia, Debasmita Pankaj Alone, Panchami Prabhakaran","doi":"10.1039/d4bm01681e","DOIUrl":"https://doi.org/10.1039/d4bm01681e","url":null,"abstract":"<p><p>Fluorescent therapeutic molecules offer a unique platform to study cellular uptake and biological pathways of drug candidates. Inhibition of the p53-HDM2 protein complex with the reactivation of the p53 pathway leading to apoptosis is a promising way to overcome the barriers and challenges in cancer therapeutic design. Although p53 helix mimetics based on the 'hotspots' design using either helical or non-helical backbones are known, cell-permeable and biocompatible inherently fluorescent helix mimetics have not yet been described. We report theragnostic helix mimetics featuring both therapeutic and bioimaging properties in a cancer cell model for the first time. The solvatochromic phthalimide unit in the scaffold functions as a site to append the hotspot mimicking residues, helps in the intramolecular hydrogen bonding mediated pre-organization of side chains on one face, and importantly, exhibits intrinsic fluorescence. The design of the mimetics, synthesis, conformational studies, and molecular docking results are discussed. <i>In vitro</i> cytotoxicity studies were carried out on four cell lines: U87MG (human glioblastoma), A549 (human non-small cell lung cancer), MDA-MB-231 (human triple-negative breast cancer) and HEK293 (non-cancerous cell line). The molecules showed anticancer activity in the micromolar range. The fluorescence properties provided valuable insights into their cellular permeability, distribution, and selectivity towards cancer cells and can shed light on their mechanisms of action.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional chrysin-loaded gallic acid–glycerol monostearate conjugate-based injectable hydrogel for targeted inhibition of hypoxia-induced NLRP3 inflammasome in ulcerative colitis†","authors":"Ajay kumar, Rahul, Kanika, Jattin Kumar, Shubham Mahajan, Aneesh Ali, Nemat Ali, Mahendra Bishnoi, Young-Ok Son and Rehan Khan","doi":"10.1039/D4BM01700E","DOIUrl":"10.1039/D4BM01700E","url":null,"abstract":"<p >Ulcerative colitis (UC) is a chronic inflammatory condition affecting the colon part of the large intestine. Since there is no cure for this disease, conventional therapies only provide symptomatic relief. Recently, phytomolecules have shown promising treatment results in various diseases. However, short half-life, hydrophobicity, and poor bioavailability limit their therapeutic potential. To overcome all these challenges, we have earlier conjugated a phytomolecule (gallic acid) (GA) with the FDA-approved generally recognized as safe (GRAS) material that is glycerol monostearate (GMS). This GA–GMS conjugate self-assembles as a hydrogel <em>via</em> the heating–cooling method and acts as a pro-drug of GA. The <em>in vivo</em> imaging results suggest that the GA–GMS hydrogel more efficiently adheres to the inflamed colon than a therapeutic enema. Additionally, it is known that the gut microbiota exaggerates UC by creating a hypoxic environment in the colon. This hypoxia is linked with NLRP3 inflammasome activation that triggers the release of IL-1β and IL-18 that downregulates MUC2 protein expression in the colon, responsible for mucin secretion in the colon. Therefore, chrysin (CR) (HIF-1α inhibitor) is encapsulated into the GA–GMS hydrogel to target hypoxia. The CR@GA–GMS hydrogel follows the enzyme-responsive release of the CR and restores DSS-induced damage to colonic tissue. Furthermore, the CR@GA–GMS hydrogel downregulates HIF-1α mediated NLRP3 inflammasome signalling while upregulating MUC2 production.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 7","pages":" 1801-1817"},"PeriodicalIF":5.8,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactobacillus-derived artificial extracellular vesicles for skin rejuvenation and prevention of photo-aging†","authors":"Seongsu Kang, Saetbyeol Jeon, Hwira Baek, Sunghwan Hwang, Seulgi Kim, Sung Hun Youn, Jin Woong Kim, Seung-Hyun Jun and Nae-Gyu Kang","doi":"10.1039/D4BM01644K","DOIUrl":"10.1039/D4BM01644K","url":null,"abstract":"<p >Extracellular vesicles (EVs) are small membrane-bound sacs released by cells that play crucial roles in intercellular communication. They transport biomolecules between cells and have both diagnostic and therapeutic potential. Artificial EVs, designed to mimic natural EVs, have been developed using various methods. In this study, <em>Lactobacillus plantarum</em> was used to create <em>Lactobacillus</em>-derived artificial EVs (LAEs) for skin rejuvenation and anti-aging. LAEs demonstrated monodispersity and effectively improved adverse gene expression and wound healing in fibroblasts. They also modulated aging-related genes and improved skin conditions in humans. Their simplicity, promptness, and lack of animal-derived sources make LAEs a promising alternative to natural EVs. LAEs have the potential to overcome the technical limitations of artificial EVs and advance EVs or exosome-based technologies for comprehensive skin rejuvenation.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 8","pages":" 2026-2035"},"PeriodicalIF":5.8,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}