Biomaterials Science最新文献_第5页

Optimization of a concentrated growth factor/mesoporous bioactive glass composite scaffold and its application in rabbit mandible defect regeneration† 浓缩生长因子/介孔生物活性玻璃复合支架的优化及其在兔下颌骨缺损修复中的应用

IF 6.6 3区医学

Biomaterials Science Pub Date : 2023-08-08 DOI: 10.1039/D3BM00805C

Mengran Ma, Wenjing Shen, Beibei Li, Mengwen Sun, Dan Lin and Lingqiang Meng

{"title":"Optimization of a concentrated growth factor/mesoporous bioactive glass composite scaffold and its application in rabbit mandible defect regeneration†","authors":"Mengran Ma, Wenjing Shen, Beibei Li, Mengwen Sun, Dan Lin and Lingqiang Meng","doi":"10.1039/D3BM00805C","DOIUrl":"https://doi.org/10.1039/D3BM00805C","url":null,"abstract":"Maxillofacial bone defect repair and regeneration remains a tremendous challenge in the field of stomatology. However, the limited osteoinductivity of artificial materials and the high cost of bioactive agents restrain their clinical translation. This study aimed to construct an economical and efficient concentrated growth factor/mesoporous bioactive glass (CGF/MBG) composite scaffold for bone regeneration. The biochemical composition and biological effects of different forms of CGFs were systematically compared, and the results showed that CGF-conditioned medium effectively promoted proliferation, migration and osteogenesis of allogenic BMSCs. Gel phase CGF (gpCGF) exhibited superior bioactivity and osteoinductivity to liquid phase CGF (lpCGF) and liquid/gel mixed phase CGF (lgpCGF), and was further applied to construct CGF/MBG scaffolds. In vitro studies demonstrated that co-culture with gpCGF-conditioned medium further enhanced the biocompatibility of MBG, increasing cell adhesion and proliferation on the scaffold. On this basis, two compositing approaches to construct the scaffold by fibrin gel formation (CGF/FG/MBG) and freeze-drying (fdCGF/MBG) were applied, and the biological efficacy of CGFs was compared in vivo. In a rabbit mandibular defect model, higher osteogenic efficiency in in situ bone regeneration of CGF/FG/MBG composite scaffolds was proved, compared with fdCGF/MBG. Taken together, the CGF/FG/MBG composite scaffold is expected to be an efficient bone repairing therapy for clinical translation, and the CGF-composited scaffold using gpCGF and the fibrin gel formation method is a promising way to enhance the bioactivity and osteoinductivity of current clinical bone repairing materials, providing new thoughts on the development of future orthopedic biomaterials.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 18","pages":" 6357-6372"},"PeriodicalIF":6.6,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6642773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Controlling differentiation of stem cells via bioactive disordered cues† 通过生物活性紊乱线索控制干细胞分化

IF 6.6 3区医学

Biomaterials Science Pub Date : 2023-08-04 DOI: 10.1039/D3BM00605K

Yujie Zhang, Murielle Rémy, Evgeny Apartsin, Emilie Prouvé, Cécile Feuillie, Christine Labrugère, Nithavong Cam and Marie-Christine Durrieu

{"title":"Controlling differentiation of stem cells via bioactive disordered cues†","authors":"Yujie Zhang, Murielle Rémy, Evgeny Apartsin, Emilie Prouvé, Cécile Feuillie, Christine Labrugère, Nithavong Cam and Marie-Christine Durrieu","doi":"10.1039/D3BM00605K","DOIUrl":"https://doi.org/10.1039/D3BM00605K","url":null,"abstract":"Ideal bone tissue engineering is to induce bone regeneration through the synergistic integration of biomaterial scaffolds, bone progenitor cells, and bone-forming factors. Biomimetic scaffolds imitate the native extracellular matrix (ECM) and are often utilized in vitro as analogues of the natural ECM to facilitate investigations of cell–ECM interactions and processes. In vivo, the cellular microenvironment has a crucial impact on regulating cell behavior and functions. A PET surface was activated and then functionalized with mimetic peptides to promote human mesenchymal stem cell (hMSC) adhesion and differentiation into an osteogenic lineage. Spray technology was used to randomly micropattern peptides (RGD and BMP-2 mimetic peptides) on the PET surface. The distribution of the peptides grafted on the surface, the roughness of the surfaces and the chemistry of the surfaces in each step of the treatment were ascertained by atomic force microscopy, fluorescence microscopy, time-of-flight secondary ion mass spectrometry, Toluidine Blue O assay, and X-ray photoelectron spectroscopy. Subsequently, cell lineage differentiation was evaluated by quantifying the expression of immunofluorescence markers: osteoblast markers (Runx-2, OPN) and osteocyte markers (E11, DMP1, and SOST). In this article, we hypothesized that a unique combination of bioactive micro/nanopatterns on a polymer surface improves the rate of morphology change and enhances hMSC differentiation. In DMEM, after 14 days, disordered micropatterned surfaces with RGD and BMP-2 led to a higher osteoblast marker expression than surfaces with a homogeneous dual peptide conjugation. Finally, hMSCs cultured in osteogenic differentiation medium (ODM) showed accelerated cell differentiation. In ODM, our results highlighted the expression of osteocyte markers when hMSCs were seeded on PET surfaces with random micropatterns.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 18","pages":" 6116-6134"},"PeriodicalIF":6.6,"publicationDate":"2023-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6642718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prussian blue nano-enzyme-assisted photodynamic therapy effectively eradicates MRSA infection in diabetic mouse skin wounds† 普鲁士蓝纳米酶辅助光动力疗法可有效根除糖尿病小鼠皮肤伤口的MRSA感染

IF 6.6 3区医学

Biomaterials Science Pub Date : 2023-08-04 DOI: 10.1039/D3BM01039B

Aidi Tong, Chunyi Tong, Jialong Fan, Jingyi Shen, Caiyun Yin, Zhou Wu, Jiansong Zhang and Bin Liu

{"title":"Prussian blue nano-enzyme-assisted photodynamic therapy effectively eradicates MRSA infection in diabetic mouse skin wounds†","authors":"Aidi Tong, Chunyi Tong, Jialong Fan, Jingyi Shen, Caiyun Yin, Zhou Wu, Jiansong Zhang and Bin Liu","doi":"10.1039/D3BM01039B","DOIUrl":"https://doi.org/10.1039/D3BM01039B","url":null,"abstract":"Antibiotic therapy can induce the generation of severe bacterial resistance, further challenging the usability of currently available drugs and treatment options. Therefore, it is essential to develop new strategies to effectively eradicate drug-resistant bacteria. Herein, we have reported a combinational strategy for the eradication of drug-resistant bacteria by using chlorin e6 (Ce6) loaded Prussian blue nanoparticles (PB NPs). This nanocomplex showed strong catalase activity and photodynamic properties. In vitro experiments demonstrated that CPB–Ce6 NPs effectively kill MRSA by generating ROS under laser irradiation. Meanwhile, the nano-enzyme activity of CPB NPs can decompose H2O2 in the bacterial microenvironment to upregulate the O2 level, which in turn alleviates hypoxia in the microenvironment and improves the antibacterial effect of PDT. In vivo results demonstrated that CPB–Ce6 NPs with laser irradiation effectively cleared MRSA and promoted infected wound repair in a diabetic mouse model and normal mice through upregulating VEGF. Moreover, CPB–Ce6 NPs showed excellent biosafety profiles in vitro and in vivo. From our point of view, this PDT based on PB NPs with nano-enzyme activity may provide an effective treatment for infections associated with drug-resistant microbes and tissue repair.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 18","pages":" 6342-6356"},"PeriodicalIF":6.6,"publicationDate":"2023-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6642772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of strontium substitution on functional activity of phosphate-based glass† 锶取代对磷酸盐基玻璃功能活性的影响

IF 6.6 3区医学

Biomaterials Science Pub Date : 2023-08-03 DOI: 10.1039/D3BM00610G

Jeong-Hyun Ryu, Utkarsh Mangal, Myung-Jin Lee, Ji-Young Seo, Il Jun Jeong, Jin-Young Park, Ji-Yeong Na, Kee-Joon Lee, Hyung-Seog Yu, Jae-Kook Cha, Jae-Sung Kwon and Sung-Hwan Choi

{"title":"Effect of strontium substitution on functional activity of phosphate-based glass†","authors":"Jeong-Hyun Ryu, Utkarsh Mangal, Myung-Jin Lee, Ji-Young Seo, Il Jun Jeong, Jin-Young Park, Ji-Yeong Na, Kee-Joon Lee, Hyung-Seog Yu, Jae-Kook Cha, Jae-Sung Kwon and Sung-Hwan Choi","doi":"10.1039/D3BM00610G","DOIUrl":"https://doi.org/10.1039/D3BM00610G","url":null,"abstract":"Phosphate-based glass (PBG) is a bioactive agent, composed of a glass network with phosphate as the primary component and can be substituted with various therapeutic ions for functional enhancement. Strontium (Sr) has been shown to stimulate osteogenic activity and inhibit pro-inflammatory responses. Despite this potential, there are limited studies that focus on the proportion of Sr substituted and its impact on the functional activity of resulting Sr-substituted PBG (PSr). In this study, focusing on the cellular biological response we synthesized and investigated the functional activity of PSr by characterizing its properties and comparing the effect of Sr substitution on cellular bioactivity. Moreover, we benchmarked the optimal composition against 45S5 bioactive glass (BG). Our results showed that PSr groups exhibited a glass structure and phosphate network like that of PBG. The release of Sr and P was most stable for PSr6, which showed favorable cell viability. Furthermore, PSr6 elicited excellent early osteogenic marker expression and inhibition of pro-inflammatory cytokine expression, which was significant compared to BG. In addition, compared to BG, PSr6 had markedly higher expression of osteopontin in immunocytochemistry, higher ALP expression in osteogenic media, and denser alizarin red staining in vitro. We also observed a comparable in vivo regenerative response in a 4-week rabbit calvaria defect model. Therefore, based on the results of this study, PSr6 could be identified as the functionally optimized composition with the potential to be applied as a valuable bioactive component of existing biomaterials used for bone regeneration.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 18","pages":" 6299-6310"},"PeriodicalIF":6.6,"publicationDate":"2023-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6642769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A CO-mediated photothermal therapy to kill drug-resistant bacteria and minimize thermal injury for infected diabetic wound healing† co介导的光热疗法杀死耐药细菌并减少感染糖尿病伤口愈合的热损伤

IF 6.6 3区医学

Biomaterials Science Pub Date : 2023-08-02 DOI: 10.1039/D3BM00774J

Xin Jin, Zelin Ou, Guowei Zhang, Rong Shi, Jumin Yang, Wenguang Liu, Gaoxing Luo, Jun Deng and Wei Wang

{"title":"A CO-mediated photothermal therapy to kill drug-resistant bacteria and minimize thermal injury for infected diabetic wound healing†","authors":"Xin Jin, Zelin Ou, Guowei Zhang, Rong Shi, Jumin Yang, Wenguang Liu, Gaoxing Luo, Jun Deng and Wei Wang","doi":"10.1039/D3BM00774J","DOIUrl":"https://doi.org/10.1039/D3BM00774J","url":null,"abstract":"With an increasing proportion of drug-resistant bacteria, photothermal therapy (PTT) is a promising alternative to antibiotic treatment for infected diabetic skin ulcers. However, the inevitable thermal damage to the tissues restricts its clinical practice. Carbon monoxide (CO), as a bioactive gas molecule, can selectively inhibit bacterial growth and promote tissue regeneration, which may be coordinated with PTT for drug-resistant bacteria killing and tissue protection. Herein, a CO-mediated PTT agent (CO@mPDA) was engineered by loading manganese carbonyl groups into mesoporous polydopamine (mPDA) nanoparticles via coordination interactions between the metal center and a catechol group. Compared to the traditional PTT, the CO-mediated PTT increases the inhibition ratio of the drug-resistant bacteria both in vitro and in diabetic wound beds by selectively inhibiting the co-chaperone of the heat shock protein 90 kDa (Hsp90), and lowers the heat resistance of the bacteria rather than the mammalian tissues. Meanwhile, the tissue-protective proteins, such as Hsp90 and vimentin (Vim), are upregulated via the WNT and PI3K-Akt pathways to reduce thermal injury, especially with a laser with a high-power density. The CO-mediated PTT unified the bacterial killing with tissue protection, which offers a promising concept to improve PTT efficiency and minimize the side-effects of PTT when treating infected skin wounds.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 18","pages":" 6236-6251"},"PeriodicalIF":6.6,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6642745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards a simple in vitro surface chemistry pre-screening method for nanoparticles to be used for drug delivery to solid tumours† 一种用于实体肿瘤药物递送的纳米颗粒的简单体外表面化学预筛选方法

IF 6.6 3区医学

Biomaterials Science Pub Date : 2023-08-02 DOI: 10.1039/D3BM00966A

Roman Schmid, Juliane Kaiser, Ramona Willbold, Nomusa Walther, Rainer Wittig and Mika Lindén

{"title":"Towards a simple in vitro surface chemistry pre-screening method for nanoparticles to be used for drug delivery to solid tumours†","authors":"Roman Schmid, Juliane Kaiser, Ramona Willbold, Nomusa Walther, Rainer Wittig and Mika Lindén","doi":"10.1039/D3BM00966A","DOIUrl":"https://doi.org/10.1039/D3BM00966A","url":null,"abstract":"An efficient nanoparticulate drug carrier intended for chemotherapy based on intravenous administration must exhibit a long enough blood circulation time, a good penetrability into the tumour volume, as well as an efficient uptake by cancer cells. Limiting factors for the therapeutic outcome in vivo are recognition of the nanoparticles as foreign objects, which triggers nanoparticle uptake by defence organs rich in macrophages, e.g. liver and spleen, on the time-scale of accumulation and uptake in/by the tumour. However, the development of nanomedicine towards efficient nanoparticle-based delivery to solid tumours is hampered by the lack of simple, reproducible, cheap, and predictive means for early identification of promising nanoparticle formulations. The surface chemistry of nanoparticles is known to be the most important determinant for the biological fate of nanoparticles, as it influences the extent of serum protein adsorption, and also the relative composition of the protein corona. Here we preliminarily evaluate an extremely simple screening method for nanoparticle surface chemistry pre-optimization based on nanoparticle uptake in vitro by PC-3 cancer cells and THP-1 macrophages. Only when both selectivity for the cancer cells as well as the extent of nanoparticle uptake are taken into consideration do the in vitro results mirror literature results obtained for small animal models. Furthermore, although not investigated here, the screening method does also lend itself to the study of actively targeted nanoparticles.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 18","pages":" 6287-6298"},"PeriodicalIF":6.6,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2023/bm/d3bm00966a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6642749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Radiation-induced photodynamic therapy using calcium tungstate nanoparticles and 5-aminolevulinic acid prodrug† 使用钨酸钙纳米颗粒和5-氨基乙酰丙酸前药†的辐射诱导光动力疗法

IF 6.6 3区医学

Biomaterials Science Pub Date : 2023-08-02 DOI: 10.1039/D3BM00921A

Dhushyanth Viswanath, Sung-Ho Shin, Jin Yoo, Sandra E. Torregrosa-Allen, Haley A. Harper, Heidi E. Cervantes, Bennett D. Elzey and You-Yeon Won

{"title":"Radiation-induced photodynamic therapy using calcium tungstate nanoparticles and 5-aminolevulinic acid prodrug†","authors":"Dhushyanth Viswanath, Sung-Ho Shin, Jin Yoo, Sandra E. Torregrosa-Allen, Haley A. Harper, Heidi E. Cervantes, Bennett D. Elzey and You-Yeon Won","doi":"10.1039/D3BM00921A","DOIUrl":"https://doi.org/10.1039/D3BM00921A","url":null,"abstract":"Photodynamic therapy (PDT) using 5-aminolevulinic acid (ALA) prodrug is a clinically tried and proven treatment modality for surface-level lesions. However, its use for deep-seated tumors has been limited due to the poor penetration depth of visible light needed to activate the photosensitizer protoporphyrin IX (PPIX), which is produced from ALA metabolism. Herein, we report the usage of poly(ethylene glycol-b-lactic acid) (PEG–PLA)-encapsulated calcium tungstate (CaWO4, CWO for short) nanoparticles (PEG–PLA/CWO NPs) as energy transducers for X-ray-activated PDT using ALA. Owing to the spectral overlap between radioluminescence afforded by the CWO core and the absorbance of PPIX, these NPs can serve as an in situ visible light activation source during radiotherapy (RT), thereby mitigating the limitation of penetration depth. We demonstrate that this effect is observed across different cell lines with varying radio-sensitivity. Importantly, both PPIX and PEG–PLA/CWO NPs exhibit no significant toxicities at therapeutic doses in the absence of radiation. To assess the efficacy of this approach, we conducted a study using a syngeneic mouse model subcutaneously implanted with inherently radio-resistant 4T1 tumors. The results show a significantly improved prognosis compared to conventional RT, even with as few as 2 fractions of 4 Gy X-rays. Taken together, these results suggest that PEG–PLA/CWO NPs are promising agents for application of ALA-PDT in deep-seated tumors, thereby significantly expanding the utility of the already established treatment strategy.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 18","pages":" 6311-6324"},"PeriodicalIF":6.6,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6642770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Site-selective post-modification of short α/γ hybrid foldamers: a powerful approach for molecular diversification towards biomedical applications† 短α/γ杂交折叠子的位点选择性后修饰:生物医学应用分子多样化的有力途径

IF 6.6 3区医学

Biomaterials Science Pub Date : 2023-08-01 DOI: 10.1039/D3BM00766A

Syed Kabir Hussain Shah, Unnati Modi, Karma Patel, Anjima James, Sreerag N, Susmita De, Rajesh Vasita and Panchami Prabhakaran

{"title":"Site-selective post-modification of short α/γ hybrid foldamers: a powerful approach for molecular diversification towards biomedical applications†","authors":"Syed Kabir Hussain Shah, Unnati Modi, Karma Patel, Anjima James, Sreerag N, Susmita De, Rajesh Vasita and Panchami Prabhakaran","doi":"10.1039/D3BM00766A","DOIUrl":"https://doi.org/10.1039/D3BM00766A","url":null,"abstract":"The extensive research work in the exhilarating area of foldamers (artificial oligomers possessing well-defined conformation in solution) has shown them to be promising candidates in biomedical research and materials science. The post-modification approach is successful in peptides, proteins, and polymers to modulate their functions. To the best of our knowledge, site-selective post-modification of a foldamer affording molecules with different pendant functional groups within a molecular scaffold has not yet been reported. We demonstrate for the first time that late-stage site-selective functionalization of short hybrid oligomers is an efficient approach to afford molecules with diverse functional groups. In this article, we report the design and synthesis of hybrid peptides with repeating units of leucine (Leu) and 5-amino salicylic acid (ASA), regioselective post-modification, conformational analyses (based on solution-state NMR, circular dichroism and computational studies) and morphological studies of the peptide nanostructures. As a proof-of-concept, we demonstrate the applications of differently modified peptides as drug delivery agents, imaging probes, and anticancer agents. The novel feature of the work is that the difference in reactivity of two phenolic OH groups in short biomimetic peptides was utilized to achieve site-selective post-modification. It is challenging to apply the same approach to short α-peptides having a poor folding tendency, and their post-functionalization may considerably affect their conformation.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 18","pages":" 6210-6222"},"PeriodicalIF":6.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6642743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A dual-sensitive nanoparticle-mediated deepening synergistic therapy strategy involving DNA damage and ICD stimuli to treat triple-negative breast cancer† 涉及DNA损伤和ICD刺激的双敏感纳米颗粒介导的深化协同治疗策略治疗三阴性乳腺癌†

IF 6.6 3区医学

Biomaterials Science Pub Date : 2023-08-01 DOI: 10.1039/D3BM00781B

Shangui Liu, Xinru Kong, Yuelin Fang, Zhijing He, Hang Wu, Jianbo Ji, Xiaoye Yang, Lei Ye and Guangxi Zhai

{"title":"A dual-sensitive nanoparticle-mediated deepening synergistic therapy strategy involving DNA damage and ICD stimuli to treat triple-negative breast cancer†","authors":"Shangui Liu, Xinru Kong, Yuelin Fang, Zhijing He, Hang Wu, Jianbo Ji, Xiaoye Yang, Lei Ye and Guangxi Zhai","doi":"10.1039/D3BM00781B","DOIUrl":"https://doi.org/10.1039/D3BM00781B","url":null,"abstract":"Triple-negative breast cancer (TNBC) is one of the most aggressive cancers with an immunosuppressive microenvironment, and achieving a satisfactory effect from monotherapies, such as chemotherapy, photodynamic therapy (PDT) or immunotherapy, remains difficult. To solve this puzzle, a deepening synergistic therapy strategy of DNA damage and immunogenic cell death (ICD) stimuli was proposed. We engineered a doxorubicin (DOX) and 4-(hydroxymethyl) phenylboronic acid pinacol ester (PBAP) prodrug polymer, and encapsulated chlorin e6 (Ce6) to obtain the hyaluronidase (HAase) and H2O2 dual-sensitive responsive nanoparticles (Ce6/HDP NPs). The NPs displayed efficient intratumoral accumulation and cellular internalization properties due to the active targeting of the hyaluronic acid (HA). The dual DNA damage of the chemotherapy and ROS production directly caused tumor cell apoptosis. The strong ICD stimuli, which were induced by ROS production and GSH depletion, generated an amplified immunogenicity to activate tumor immunotherapy in vivo. In this manner, the NPs could significantly inhibit primary tumor, abscopal tumor, pulmonary metastasis and recurrent tumor in a subcutaneous 4T1 tumor model, with effective biosafety. This study has provided a promising deepening synergistic therapy strategy against TNBC.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 18","pages":" 6325-6341"},"PeriodicalIF":6.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6642771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Matrisomal components involved in regenerative wound healing in axolotl and Acomys: implications for biomaterial development† 参与蝾螈和蝾螈再生伤口愈合的基质成分:对生物材料发展的影响

IF 6.6 3区医学

Biomaterials Science Pub Date : 2023-08-01 DOI: 10.1039/D3BM00835E

Nancy Avila-Martinez, Merel Gansevoort, Juul Verbakel, Haarshaadri Jayaprakash, Ines Maria Araujo, Marta Vitorino, Gustavo Tiscornia, Toin H. van Kuppevelt and Willeke F. Daamen

{"title":"Matrisomal components involved in regenerative wound healing in axolotl and Acomys: implications for biomaterial development†","authors":"Nancy Avila-Martinez, Merel Gansevoort, Juul Verbakel, Haarshaadri Jayaprakash, Ines Maria Araujo, Marta Vitorino, Gustavo Tiscornia, Toin H. van Kuppevelt and Willeke F. Daamen","doi":"10.1039/D3BM00835E","DOIUrl":"https://doi.org/10.1039/D3BM00835E","url":null,"abstract":"Achieving regeneration in humans has been a long-standing goal of many researchers. Whereas amphibians like the axolotl (Ambystoma mexicanum) are capable of regenerating whole organs and even limbs, most mammals heal their wounds via fibrotic scarring. Recently, the African spiny mouse (Acomys sp.) has been shown to be injury resistant and capable of regenerating several tissue types. A major focal point of research with Acomys has been the identification of drivers of regeneration. In this search, the matrisome components related to the extracellular matrix (ECM) are often overlooked. In this review, we compare Acomys and axolotl skin wound healing and blastema-mediated regeneration by examining their wound healing responses and comparing the expression pattern of matrisome genes, including glycosaminoglycan (GAG) related genes. The goal of this review is to identify matrisome genes that are upregulated during regeneration and could be potential candidates for inclusion in pro-regenerative biomaterials. Research papers describing transcriptomic or proteomic coverage of either skin regeneration or blastema formation in Acomys and axolotl were selected. Matrisome and GAG related genes were extracted from each dataset and the resulting lists of genes were compared. In our analysis, we found several genes that were consistently upregulated, suggesting possible involvement in regenerative processes. Most of the components have been implicated in regulation of cell behavior, extracellular matrix remodeling and wound healing. Incorporation of such pro-regenerative factors into biomaterials may help to shift pro-fibrotic processes to regenerative responses in treated wounds.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 18","pages":" 6060-6081"},"PeriodicalIF":6.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2023/bm/d3bm00835e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6642715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1