Biomaterials Science最新文献

{"title":"Leveraging microtopography to pattern multi-oriented muscle actuators.","authors":"Tamara Rossy, Laura Schwendeman, Sonika Kohli, Maheera Bawa, Pavankumar Umashankar, Roi Habba, Oren Tchaicheeyan, Ayelet Lesman, Ritu Raman","doi":"10.1039/d4bm01017e","DOIUrl":"10.1039/d4bm01017e","url":null,"abstract":"<p><p>Engineering skeletal muscle tissue with precisely defined alignment is of significant importance for applications ranging from drug screening to biohybrid robotics. Aligning 2D contractile muscle monolayers, which are compatible with high-content imaging and can be deployed in planar soft robots, typically requires micropatterned cues. However, current protocols for integrating microscale topographical features in extracellular matrix hydrogels require expensive microfabrication equipment and multi-step procedures involving error-prone manual handling steps. To address this challenge, we present STAMP (simple templating of actuators <i>via</i> micro-topographical patterning), an easily accessible and cost-effective one-step method to pattern microtopography of various sizes and configurations on the surface of hydrogels using reusable 3D printed stamps. We demonstrate that STAMP enables precisely controlling the alignment of mouse and human skeletal muscle fibers without negatively impacting their maturation or function. To showcase the versatility of our technique, we designed a planar soft robot inspired by the iris, which leverages spatially segregated regions of concentric and radial muscle fibers to control pupil dilation. Optogenetic skeletal muscle fibers grown on a STAMPed iris substrates formed a multi-oriented actuator, and selective light stimulation of the radial and concentric fibers was used to control the function of the iris, including pupil constriction. Computational modeling of the biohybrid robot as an active bilayer matched experimental outcomes, showcasing the robustness of our STAMP method for designing, fabricating, and testing planar biohybrid robots capable of complex multi-DOF motion.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907412/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143622808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Casein microparticles filled with cellulase to enzymatically degrade nanocellulose for cell growth†","authors":"Céline Bastard, Jann Carl-Theodor Schulte, Md Asaduzzaman, Calvin Hohn, Yonca Kittel, Laura De Laporte and Ronald Gebhardt","doi":"10.1039/D4BM01508H","DOIUrl":"10.1039/D4BM01508H","url":null,"abstract":"<p >For tissue engineering, nanocellulose-based three-dimensional hydrogel structures hold potential as biocompatible support materials for biomimetic scaffolds to regenerate damaged tissues. One challenge of this material is that nanocellulose does not degrade in the human body. Therefore, different carriers are needed to locally deliver cellulase in a controlled manner to degrade the scaffold at the same time the cells grow and proliferate. To achieve this, we developed casein microparticles (CMPs) as delivery vehicles as they are non-toxic and have high porosity with a stable structure at physiological pH values. The porosity of the CMPs was first tested by diffusion experiments with fluorescently labelled dextrans of different sizes as model molecules, demonstrating inward diffusion of dextrans up to 500 kDa. The CMPs continuously release active cellulase, resulting in the degradation of the nanocellulose hydrogel over a time of 21 days, supporting 3D cell growth.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 9","pages":" 2435-2443"},"PeriodicalIF":5.8,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d4bm01508h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Snowflake-like Cu2O–Pt nanocluster-mediated Fenton photothermal and chemodynamic therapy for antibiotic wound healing†","authors":"En Li, Qi Han, Ting Chen, Si Cheng and Jinghua Li","doi":"10.1039/D5BM00096C","DOIUrl":"10.1039/D5BM00096C","url":null,"abstract":"<p >The Fenton reaction serves as the fundamental mechanism behind chemodynamic therapy (CDT), wherein highly reactive hydroxyl radicals (˙OH) are produced to efficiently induce bacterial cell death. On the other hand, photothermal therapy (PTT) utilizes photosensitizers to absorb specific wavelengths of light, generating localized heat that disrupts bacterial cell membranes, leading to bactericidal effects. In this study, platinum nanoparticles (PtNPs) were successfully doped onto the surface of hexapodal cuprous oxide (HCu<small>₂</small>O), resulting in the synthesis of hexapodal snowflake-like Cu<small>₂</small>O–Pt nanoparticles (HCPNLs). These HCPNLs synergistically combine the mechanisms of CDT and PTT, significantly enhancing antibacterial efficacy. <em>In vitro</em> antimicrobial experiments have demonstrated that HCPNLs exhibit strong antimicrobial activity against both Gram-positive <em>Staphylococcus aureus</em> (<em>S. aureus</em>) and Gram-negative <em>Escherichia coli</em> (<em>E. coli</em>). Additionally, HCPNLs effectively disrupted biofilm formation and improved tissue penetration. In a murine model of mixed bacterial infection, HCPNLs showed excellent synergistic antimicrobial effects, significantly promoting wound healing with minimal toxicity. Overall, the unique properties of HCPNLs provide a novel option for non-resistant antimicrobial therapy in biomedical applications.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 9","pages":" 2394-2409"},"PeriodicalIF":5.8,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in non-invasive in vivo tracking of cell-based cancer immunotherapies","authors":"Anika D. Kulkarni, Tasneem Mukarrama, Brendan R. Barlow and Jinhwan Kim","doi":"10.1039/D4BM01677G","DOIUrl":"10.1039/D4BM01677G","url":null,"abstract":"<p >Immunotherapy has been at the forefront of cancer treatment research in recent years due to an increased understanding of the immune system's role in cancer and the substantial benefits it has demonstrated compared to conventional treatment methods. In particular, immune cell-based approaches utilizing T cells, natural killer (NK) cells, macrophages, and more have shown great potential as cancer treatments. While these treatments hold promise, there are still numerous issues that limit their clinical translation, including a lack of understanding of their mechanisms and inconsistent responses to treatment. Traditionally, tissue or blood samples are collected as a means of monitoring treatment progression. However, these <em>in vitro</em> diagnostics are invasive and provide limited information about the real-time status of the treatment or its long-term effectiveness. To address these limitations, novel non-invasive imaging modalities have been developed. These include optical imaging, X-ray computed tomography (CT), magnetic resonance imaging (MRI), positron emission tomography (PET) and single-photon emission computed tomography (SPECT), and photoacoustic (PA) imaging. This review focuses on methods for tracking cell-based cancer immunotherapies using these <em>in vivo</em> imaging modalities, thereby enhancing real-time monitoring of their therapeutic effect and predictions of their long-term efficacy.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 8","pages":" 1939-1959"},"PeriodicalIF":5.8,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing tissue engineering through vascularized cell spheroids: building blocks of the future","authors":"Chunxiang Lu, Aoxiang Jin, Huazhen Liu, Chuang Gao, Wenbin Sun, Yi Zhang, Qiqi Dai and Yuanyuan Liu","doi":"10.1039/D4BM01206B","DOIUrl":"10.1039/D4BM01206B","url":null,"abstract":"<p >Vascularization is a crucial aspect of biofabrication, as the development of vascular networks is essential for tissue survival and the optimization of cellular functions. Spheroids have emerged as versatile units for vascularization, demonstrating significant potential in angiogenesis and prevascularization for tissue engineering and regenerative medicine. However, a major challenge in creating customized vascularized spheroids is the construction of a biomimetic extracellular matrix (ECM) microenvironment. This process requires careful regulation of environmental factors, including the modulation of growth factors, the selection of culture media, and the co-culture of diverse cell types. Recent advancements in biofabrication have expanded the potential applications of vascularized spheroids. The integration of microfluidic technology with bioprinting offers promising solutions to existing challenges in regenerative medicine. Spheroids have been widely studied for their ability to promote vascularization in <em>in vitro</em> models. This review highlights the latest developments in vascularized biofabrication, and systematically explores strategies for constructing vascularized spheroids. We provide a comprehensive analysis of spheroid applications in specific tissues, including skin, liver, bone, cardiac, and tumor models. Finally, the review addresses the major challenges and future directions in the field.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 8","pages":" 1901-1922"},"PeriodicalIF":5.8,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing nano-immunotherapy of cancer through cGAS–STING pathway modulation","authors":"Gaohong Fu, Yanan Zhao, Chengqiong Mao and Yang Liu","doi":"10.1039/D4BM01532K","DOIUrl":"10.1039/D4BM01532K","url":null,"abstract":"<p >Activation of the cyclic guanosine monophosphate–adenosine monophosphate synthase (cGAS)–stimulator of interferon genes (STING) pathway plays a critical role in cancer immunotherapy due to the secretion of multiple pro-inflammatory cytokines and chemokines. Numerous cGAS–STING agonists have been developed for preclinical and clinical trials in tumor immunity. However, several obstacles, such as agonist molecules requiring multiple doses, rapid degradation and poor targeting, weaken STING activation at the tumor site. The advancement of nanotechnology provides an optimized platform for the clinical application of STING agonists. In this review, we summarize events of cGAS–STING pathway activation, the dilemma of delivering STING agonists, and recent advances in the nano-delivery of cGAS–STING agonist formulations for enhancing tumor immunity. Furthermore, we address the future challenges associated with STING-based therapies and offer insights to guide subsequent clinical applications.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 9","pages":" 2235-2260"},"PeriodicalIF":5.8,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143661624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wear-resistant antibacterial UHMWPE-based implant materials obtained by radiation crosslinking†","authors":"Nicoletta Inverardi, Maria F. Serafim, Amita Sekar, Keita Fujino, Matheus Ferreira, Anthony Marzouca, Emma Nagler, Orhun K. Muratoglu and Ebru Oral","doi":"10.1039/D4BM01663G","DOIUrl":"10.1039/D4BM01663G","url":null,"abstract":"<p >The crosslinking of ultrahigh molecular weight polyethylenes (UHMWPEs) by irradiation has been employed for decades to enhance the wear resistance of these materials when used as a load-bearing implant component for joint arthroplasty. This surgical procedure can restore the mobility of patients affected by severe arthritis by the implantation of an artificial joint made of an articulating pair and a bearing component. While the surgery is usually successful, one of the most severe complications is peri-prosthetic joint infection (PJI), which can be extremely difficult to treat and eradicate. The use of UHMWPEs as a platform for the local delivery of antibiotics in addition to their structural function could be extremely beneficial for the improvement in the outcome of PJIs. In this study, we investigated whether irradiation can be used to sterilize and crosslink antibiotic-loaded UHMWPEs, and its effect on the drug eluting and antibacterial properties of these materials. We found that the antibiotics gentamicin sulfate and vancomycin hydrochloride were stable in irradiated UHMWPEs and did not hinder crosslinking of the UHMWPE matrix. Effective crosslinking led to optimal wear resistance, which was comparable to that of clinically available UHMWPEs. Sustained drug release was observed for an extended duration (up to six months) and both the drug eluents and eluted material surfaces showed antibacterial activity against <em>Staphylococcus aureus</em>, the most common causative bacterium for PJIs.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 9","pages":" 2422-2434"},"PeriodicalIF":5.8,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Membrane-intercalating conjugated oligoelectrolytes for lipid membrane imaging","authors":"Lingna Wang, Zehua Chen, Zhaohui Dai, Meng Li, Bo Chen and Bing Wang","doi":"10.1039/D5BM00028A","DOIUrl":"10.1039/D5BM00028A","url":null,"abstract":"<p >Membrane-intercalating conjugated oligoelectrolytes (MICOEs), a class of phospholipid bilayer mimics, demonstrate an exceptional ability to spontaneously integrate into biological membranes through a combination of electrostatic and hydrophobic interactions. This unique property makes them promising candidates for membrane imaging applications. Over the past decade, MICOEs have been successfully applied to imaging and tracking a wide range of biological membranes, including microbial membranes, mammalian plasma membranes, intracellular membranes, extracellular vesicles, and artificial liposomes. Recent advancements have shed light on the imaging mechanisms of MICOEs and highlighted their potential as fluorescent probes, with a focus on structural optimization to enhance their performance. Building on these developments, this review will explore the intercalation mechanisms of MICOEs, analyze the structure–activity relationships governing their molecular design and imaging capabilities, and discuss the future challenges and emerging opportunities for their application as advanced membrane dyes.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 8","pages":" 1923-1938"},"PeriodicalIF":5.8,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143622810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorinated poly(aryl ether)/polypropylene composite patch for prevention of abdominal adhesions after hernia repairs†","authors":"Wenqing Zhang, Huawei Yang, Xu Zhang, Zhaoyang Wang and Shifang Luan","doi":"10.1039/D4BM01704H","DOIUrl":"10.1039/D4BM01704H","url":null,"abstract":"<p >Hernia typically does not heal spontaneously. Large-pore patches, most notably polypropylene patches (PP patches), are the gold standard in hernia repair surgery. However, a single patch is insufficient for both anti-adhesion and tissue fusion, leading to complications such as organ adhesions. In this study, a chemically stable and biocompatible modified fluorinated poly(aryl ether) (FPAE-F) was prepared by grafting perfluoroalkyl groups onto a fluorinated poly(aryl ether) <em>via</em> nucleophilic aromatic substitution. A porous FPAE-F fiber film (eFPAE-F) was fabricated by electrospinning and combined with a PP patch to produce a modified fluorinated poly(aryl ether)/polypropylene (FPAE-F/PP) composite patch. The eFPAE-F layer of the composite patch, which faces the abdominal viscera, exhibits a water contact angle of 151.3 ± 1.2°. This superhydrophobic surface prevents protein adhesion, thereby inhibiting rapid fibroblast proliferation. The small pore size (3.22 ± 1.25 μm) of the eFPAE-F layer effectively impedes fibroblast infiltration while permitting the transport and metabolism of nutrients. <em>In vivo</em> experiments have demonstrated that the composite patch is a viable anti-adhesion material, resulting in no adhesions and low inflammation levels after 2 weeks. Due to its outstanding anti-adhesion properties, eFPAE-F/PP is expected to be applied in the field of hernia repair.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 8","pages":" 2134-2141"},"PeriodicalIF":5.8,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143622807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polycaprolactone/sodium alginate coaxial wet-spun fibers modified with carbon nanofibers and ceftazidime for improved clotting and infection control in wounds†","authors":"Elina Marinho, Beatriz M. Silva, Catarina S. Miranda, Sonia L. C. Pinho and Helena P. Felgueiras","doi":"10.1039/D4BM01667J","DOIUrl":"10.1039/D4BM01667J","url":null,"abstract":"<p >Chronic wounds (CWs) are a significant public health concern and affect 1–2% of the world's population. They are responsible for high morbidity and mortality rates. Bacterial infections caused by <em>Staphylococcus aureus</em> and <em>Pseudomonas aeruginosa</em> are very common in CWs and prevent normal wound healing steps from taking place. Carbon nanofibers (CNFs) have attracted interest due to their inherent antibacterial and blood clotting abilities, as well as mechanical strength. The aim of this research was to engineer coaxial fibers by wet-spinning as new platforms for drug delivery in CW care (promoting rapid blood clotting and consequent tissue regeneration). Coaxial fibers were produced with an outer layer (shell) made of a mechanically resilient polycaprolactone (PCL at 10 wt%) reinforced with carbon nanofibers (CNFs at 50, 100, and 150 μg mL<small>⁻¹</small>), while the inner layer (core) was made of a highly hydrated mixture of 2 wt% sodium alginate (SA) loaded with ceftazidime (CZ) at 128 μg mL<small>⁻¹</small> (minimum bactericidal concentration). The fibers’ double-layer structure was verified by scanning electron microscopy. Core–shell fibers were deemed highly flexible and mechanically resilient and resistant to rupture, with such properties being improved with the incorporation of CNFs. Most fibers preserved their structural integrity after 28 days of incubation in physiological-like medium. Furthermore, data reported the ability of CZ combined with CNFs to fight microbial proliferation and showed that the presence of CNFs promoted blood clotting, with PCL/CNFs50 being the most effective from the group. It was found that higher concentrations of CNFs had a detrimental effect, highlighting a concentration-dependent response. The presence of PLC in the fibers resulted in a mitigation of the CNFs’ cytotoxic impact on keratinocytes. The incorporation of CZ had no effect on the metabolic activity of the cells. Overall, the results demonstrated the potentialities of the engineered coaxial fibers for applications in wound care.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 8","pages":" 2047-2065"},"PeriodicalIF":5.8,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}