Biomaterials Science最新文献_第4页

Amplified response of drug-induced liver fibrosis via immune cell co-culture in a 3D in vitro hepatic fibrosis model. 在三维体外肝纤维化模型中通过免疫细胞共培养增强药物诱导的肝纤维化反应。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-11-01 DOI: 10.1039/d4bm00874j

Hyewon Jung, Mi-Lang Kyun, Ji-In Kwon, Jeongha Kim, Ju-Kang Kim, Daeui Park, Yu Bin Lee, Kyoung-Sik Moon

{"title":"Amplified response of drug-induced liver fibrosis via immune cell co-culture in a 3D in vitro hepatic fibrosis model.","authors":"Hyewon Jung, Mi-Lang Kyun, Ji-In Kwon, Jeongha Kim, Ju-Kang Kim, Daeui Park, Yu Bin Lee, Kyoung-Sik Moon","doi":"10.1039/d4bm00874j","DOIUrl":"https://doi.org/10.1039/d4bm00874j","url":null,"abstract":"Liver fibrosis, a critical consequence of chronic liver diseases, is characterized by excessive extracellular matrix (ECM) deposition driven by inflammation. This process involves complex interactions among hepatocytes, hepatic stellate cells (HSCs), and Kupffer cells, the liver's resident macrophages. Kupffer cells are essential in initiating fibrosis through the release of pro-inflammatory cytokines that activate HSCs. Although various in vitro liver fibrosis models have been developed, there is a lack of models that include the immune environment of the liver to clarify the influence of immune cells on the progression of liver fibrosis. We developed an in vitro liver fibrosis model by co-culturing hepatocytes (HepaRG), a hepatic stellate cell line (LX-2), and macrophages (differentiated THP-1). The effects of liver fibrosis inducers, transforming growth factor-beta1 (TGF-β1) and methotrexate (MTX), on the inflammatory response and stellate cell activation were evaluated in this triple co-culture model. A triple co-culture condition was developed as a 3D in vitro model using gelatin methacrylate (GelMA), offering a more biomimetic environment and achieving liver fibrosis via immune cell activation associated ECM deposition. In this study, the developed triple co-culture model has the potential to elucidate cell progression roles in liver fibrosis and can be applied in drug screening and toxicity assessments targeting liver fibrosis.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formulation of lipid nanoparticles containing ginsenoside Rg2 and protopanaxadiol for highly efficient delivery of mRNA. 含有人参皂苷 Rg2 和原人参皂苷的脂质纳米颗粒的配方，用于高效传递 mRNA。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-10-31 DOI: 10.1039/d4bm01070a

Sin A Park, Dajeong Hwang, Jae Hoon Kim, Seung-Yeul Lee, Jaebeom Lee, Han Sang Kim, Kyung-A Kim, Bumhee Lim, Jae-Eon Lee, Yong Hyun Jeon, Tae Jeong Oh, Jaewook Lee, Sungwhan An

{"title":"Formulation of lipid nanoparticles containing ginsenoside Rg2 and protopanaxadiol for highly efficient delivery of mRNA.","authors":"Sin A Park, Dajeong Hwang, Jae Hoon Kim, Seung-Yeul Lee, Jaebeom Lee, Han Sang Kim, Kyung-A Kim, Bumhee Lim, Jae-Eon Lee, Yong Hyun Jeon, Tae Jeong Oh, Jaewook Lee, Sungwhan An","doi":"10.1039/d4bm01070a","DOIUrl":"10.1039/d4bm01070a","url":null,"abstract":"Lipid nanoparticles (LNPs) are widely recognized as crucial carriers of mRNA in therapeutic and vaccine development. The typical lipid composition of mRNA-LNP systems includes an ionizable lipid, a helper lipid, a polyethylene glycol (PEG)-lipid, and cholesterol. Concerns arise regarding cholesterol's susceptibility to oxidation, potentially leading to undesired immunological responses and toxicity. In this study, we formulated novel LNPs by replacing cholesterol with phytochemical-derived compounds, specifically ginsenoside Rg2 and its derivative phytosterol protopanaxadiol (PPD), and validated their efficacy as mRNA delivery systems. The mRNA-LNP complexes were manually prepared through a simple mixing process. The biocompatibility of these Rg2-based LNPs (Rg2-LNP) and PPD-based LNPs (PPD-LNP) was assessed through cell viability assays, while the protective function of LNPs for mRNA was demonstrated by RNase treatment. Enhanced green fluorescent protein (EGFP) mRNA delivery and expression in A549 and HeLa cells were analyzed using optical microscopy and flow cytometry. The expression efficiency of Rg2-LNP and PPD-LNP was compared with that of commercially available LNPs, with both novel formulations demonstrating superior transfection and EGFP expression. Furthermore, in vivo tests following intramuscular (I.M.) injection in hairless mice demonstrated efficient luciferase (Luc) mRNA delivery and effective Luc expression using Rg2-LNP and PPD-LNP compared to commercial LNPs. Results indicated that the efficiency of EGFP and Luc expression in Rg2-LNP and PPD-LNP surpassed that of the cholesterol-based LNP formulation. These findings suggest that Rg2-LNP and PPD-LNP are promising candidates for future drug and gene delivery systems.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aqueous based ultra-small magnetic Cr-doped CdSe quantum dots as a potential dual imaging probe in biomedicine. 水基超小型磁性铬掺杂碲化镉量子点作为生物医学中一种潜在的双重成像探针。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-10-31 DOI: 10.1039/d4bm00811a

Shamili Bandaru, Nilja George, Bhargy Sharma, Mathangi Palanivel, Arunima Mukherjee, Wen-Ya Wu, Krishna Kanta Ghosh, Writoban Basu Ball, Balazs Gulyas, Parasuraman Padmanabhan, Siddhartha Ghosh, Sabyasachi Chakrabortty

{"title":"Aqueous based ultra-small magnetic Cr-doped CdSe quantum dots as a potential dual imaging probe in biomedicine.","authors":"Shamili Bandaru, Nilja George, Bhargy Sharma, Mathangi Palanivel, Arunima Mukherjee, Wen-Ya Wu, Krishna Kanta Ghosh, Writoban Basu Ball, Balazs Gulyas, Parasuraman Padmanabhan, Siddhartha Ghosh, Sabyasachi Chakrabortty","doi":"10.1039/d4bm00811a","DOIUrl":"10.1039/d4bm00811a","url":null,"abstract":"The substitution of semiconductor quantum dots (QDs) by a small number of transition-metal ions with magnetic properties gives rise to magnetic-doped semiconductors. With a balance of optical and magnetic properties, these magnetic semiconductors are widely used in spintronics, bioimaging and magnetic resonance imaging (MRI) applications. To facilitate their usage in bio-applications, it is critical to synthesize water-soluble magnetic QDs with a stabilized structure while maintaining their optical and magnetic properties. Here in our work, we have developed a facile substituted synthetic route to achieve Cr-doped CdSe (Cr-CdSe) via hydrothermal method. The effects of doping on the structural, optical, and magnetic properties of Cr-CdSe were studied using X-ray diffraction, UV-visible spectroscopy, and photoluminescence lifetime. We then explored their chemical nature and change in morphology with an increase in doping concentration via X-ray photoelectron spectroscopy and transmission electron microscopy. Water-soluble QDs have been used as bioimaging probes for the past few decades due to their strong fluorescence, photostability and improved tissue or cellular penetration. However, incorporating magnetic material into a fluorescent entity harnesses the ability to control the strengths of both modalities, which enhances diagnostic accuracy and facilitates its application in bio-systems, especially in early accurate diagnosis. Finally, we demonstrate the competency of Cr-CdSe as a dual-imaging probe with fluorescent cellular imaging and MRI applications.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Tobramycin-mediated self-assembly of DNA nanostructures for targeted treatment of Pseudomonas aeruginosa-infected lung inflammation 更正：托布霉素介导的 DNA 纳米结构自组装用于靶向治疗铜绿假单胞菌感染的肺部炎症。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-10-29 DOI: 10.1039/D4BM90084G

Yuhang Xu, Qian Liu, Bin Wang, Quan Li, Yue Chen, Yao Yang, Zhihao Zhu, Daohui Gong, Chuan Zhang, Guansong Wang and Hang Qian

引用次数: 0

Correction: The improved targeting of an aspirin prodrug albumin-based nanosystem for visualizing and inhibiting lung metastasis of breast cancer 更正：改进阿司匹林原药白蛋白纳米系统的靶向性，用于观察和抑制乳腺癌的肺转移。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-10-24 DOI: 10.1039/D4BM90077D

Wancun Zhang, Lili Xia, Xiangyu Ren, Mengyuan Cui, Tianguang Liu, Chen Ling, Yanqi Xu, Dawei Deng, Xianwei Zhang, Yueqing Gu and Peng Wang

引用次数: 0

Sustainable and durable color cosmetics: riboflavin phosphate-mediated photo-crosslinked casein films with tannic acid† 可持续和耐久的彩妆：以磷酸核黄素为介质的单宁酸光交联酪蛋白薄膜。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-10-24 DOI: 10.1039/D4BM01254B

Min Ji Hong, Yerin Lee, Su Jin Kyung, Joonho Choi and Hyun Jong Lee

{"title":"Sustainable and durable color cosmetics: riboflavin phosphate-mediated photo-crosslinked casein films with tannic acid†","authors":"Min Ji Hong, Yerin Lee, Su Jin Kyung, Joonho Choi and Hyun Jong Lee","doi":"10.1039/D4BM01254B","DOIUrl":"10.1039/D4BM01254B","url":null,"abstract":"The cosmetics industry is increasingly focusing on developing sustainable and environmentally friendly products while maintaining high performance. In color cosmetics, achieving long-lasting durability of water-soluble dyes remains a challenge. This study presents a sustainable approach to enhance the durability of water-soluble dyes in cosmetics using biopolymer-based films. The casein films were fabricated through riboflavin phosphate (RFP)-mediated photo-crosslinking, with tannic acid (TA) incorporated to improve mechanical properties. The fabrication process, characterization, and performance evaluation of the biopolymer-based films were investigated. Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) analyses confirmed the successful crosslinking and formation of a porous network structure. Rheological measurements revealed that the incorporation of TA significantly enhanced the mechanical strength of the films. Cytocompatibility assessment using NIH/3T3 fibroblasts demonstrated the films’ excellent biocompatibility. The durability and color retention of a water-soluble red dye in the biopolymer-based films were evaluated on human skin. The films formed under blue light irradiation exhibited superior dye retention compared to non-irradiated films, with TA addition providing a minor improvement in durability. This study bridges the gap between cosmetic science and biomaterials research, providing a foundation for future investigations into bio-interactive materials for dermal applications. These findings highlight the potential of RFP-mediated photo-crosslinked casein films as a sustainable and effective solution for enhancing the durability of water-soluble dyes in color cosmetics.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 23","pages":" 6136-6147"},"PeriodicalIF":5.8,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradable fluorescent protein chromophore nanoparticles for hypoxic two-photon photodynamic therapy† 用于缺氧双光子光动力疗法的可生物降解荧光蛋白发色团纳米粒子。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-10-23 DOI: 10.1039/D4BM01162G

Wan Feng and Ying Qian

{"title":"Biodegradable fluorescent protein chromophore nanoparticles for hypoxic two-photon photodynamic therapy†","authors":"Wan Feng and Ying Qian","doi":"10.1039/D4BM01162G","DOIUrl":"10.1039/D4BM01162G","url":null,"abstract":"In this paper, biodegradable red fluorescent protein (RFP) chromophore analogue DPFP-SS-FA nanoparticles were synthesized for hypoxic two-photon photodynamic therapy. The maximum emission wavelength of DPFP-SS-FA is in the red-to-near-infrared region at 674 nm. Interestingly, these DPFP-SS-FA nanoparticles remain stable under physiological conditions, but deplete glutathione and disintegrate into the RFP chromophore analogue monomer in the tumor microenvironment. Meanwhile, electron paramagnetic resonance data have shown that DPFP-SS-FA produced enhanced 1O2/O2˙− signals after glutathione depletion causing an enhanced PDT effect. DPFP-SS-FA has negligible cell dark toxicity and high phototoxicity in hypoxic environments, indicating the outstanding hypoxia-overcoming ability of DPFP-SS-FA. In addition, due to its folic acid receptor and lysosome dual-targeting ability, DPFP-SS-FA is highly enriched in A-549 tumor cells. In particular, the hypoxic two-photon photodynamic therapy mediated by DPFP-SS-FA nanoparticles was validated in a zebrafish tumor model. Under 800 nm two-photon excitation, DPFP-SS-FA enabled bright two-photon fluorescence imaging and significantly inhibited the growth of tumor cells in zebrafish. The biodegradable DPFP-SS-FA nanoparticles reasonably constructed in this study can serve as excellent candidates for efficient hypoxic two-photon photosensitizers to treat deep tumor tissues.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 23","pages":" 6123-6135"},"PeriodicalIF":5.8,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of nanobodies with conformational specificity for tau oligomers that recognize tau aggregates from human Alzheimer's disease samples† 生成具有构象特异性的纳米抗体，用于识别来自人类阿尔茨海默氏症样本的 tau 低聚物。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-10-22 DOI: 10.1039/D4BM00707G

Nikki McArthur, Jay D. Squire, Ogechukwu J. Onyeachonam, Nemil N. Bhatt, Cynthia Jerez, Abigail L. Holberton, Peter M. Tessier, Levi B. Wood, Rakez Kayed and Ravi S. Kane

{"title":"Generation of nanobodies with conformational specificity for tau oligomers that recognize tau aggregates from human Alzheimer's disease samples†","authors":"Nikki McArthur, Jay D. Squire, Ogechukwu J. Onyeachonam, Nemil N. Bhatt, Cynthia Jerez, Abigail L. Holberton, Peter M. Tessier, Levi B. Wood, Rakez Kayed and Ravi S. Kane","doi":"10.1039/D4BM00707G","DOIUrl":"10.1039/D4BM00707G","url":null,"abstract":"Tauopathies are neurodegenerative diseases that involve tau misfolding and aggregation in the brain. These diseases, including Alzheimer's disease (AD), are some of the least understood and most difficult to treat neurodegenerative disorders. Antibodies and antibody fragments that target tau oligomers, which are especially toxic forms of tau, are promising options for immunotherapies and diagnostic tools for tauopathies. In this study, we have developed conformational, tau oligomer-specific nanobodies, or single-domain antibodies. We demonstrate that these nanobodies, OT2.4 and OT2.6, are highly specific for tau oligomers relative to tau monomers and fibrils. We used epitope mapping to verify that these nanobodies bind to discontinuous epitopes on tau and to support the idea that they interact with a conformation present in the oligomeric, and not monomeric or fibrillar, forms of tau. We show that these nanobodies interact with tau oligomers in brain samples from AD patients and from healthy older adults with primary age-related tauopathy. Our results demonstrate the potential of these nanobodies as tau oligomer-specific binding reagents and future tauopathy therapeutics and diagnostics.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 23","pages":" 6033-6046"},"PeriodicalIF":5.8,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142453572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous inhibition of heat shock proteins and autophagy enhances radiofrequency ablation of hepatocellular carcinoma† 同时抑制热休克蛋白和自噬可增强肝细胞癌的射频消融。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-10-21 DOI: 10.1039/D4BM01190B

Jinchao Zhao, Lei Lei, Wenbin Dai, Angfeng Jiang, Qiao Jin and Zhe Tang

{"title":"Simultaneous inhibition of heat shock proteins and autophagy enhances radiofrequency ablation of hepatocellular carcinoma†","authors":"Jinchao Zhao, Lei Lei, Wenbin Dai, Angfeng Jiang, Qiao Jin and Zhe Tang","doi":"10.1039/D4BM01190B","DOIUrl":"10.1039/D4BM01190B","url":null,"abstract":"Radiofrequency ablation (RFA) is a commonly used minimally invasive treatment for hepatocellular carcinoma (HCC). However, incomplete radiofrequency ablation (iRFA) promotes tumor progression and metastasis. There is an urgent need to develop innovative strategies to enhance the efficacy of iRFA. The upregulation of heat shock proteins (HSPs) and activation of protective autophagy in tumor cells upon exposure to sublethal heat enhance the thermotolerance, thereby promoting tumor cell survival. Here, 3-methyladenine (3-MA) and lonidamine (LND) co-encapsulated liposomes (Lip@LND/3-MA) are designed to enhance the efficacy of iRFA by simultaneous inhibition of glycolysis and autophagy. On one hand, LND inhibits hexokinase, a key enzyme in glycolysis, and thus reduces ATP production and consequently suppresses the expression of HSPs. On the other hand, 3-MA, as an autophagy inhibitor, can inhibit protective autophagy after iRFA. Lip@LND/3-MA is confirmed to suppress the expression of HSPs and reduce the autophagy level during RFA. Therefore, the thermotolerance of tumor cells is significantly weakened, leading to remarkably enhanced therapeutic efficacy of iRFA. It is believed that simultaneous inhibition of HSPs and autophagy is a promising therapeutic strategy in clinical practice of RFA.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 23","pages":" 6082-6098"},"PeriodicalIF":5.8,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142453584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthetic and biological nanoparticles for cancer immunotherapy† 用于癌症免疫疗法的合成和生物纳米粒子。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-10-18 DOI: 10.1039/D4BM00995A

Inês Oliveira, Paulo Rodrigues-Santos, Lino Ferreira and Ricardo Pires das Neves

{"title":"Synthetic and biological nanoparticles for cancer immunotherapy†","authors":"Inês Oliveira, Paulo Rodrigues-Santos, Lino Ferreira and Ricardo Pires das Neves","doi":"10.1039/D4BM00995A","DOIUrl":"10.1039/D4BM00995A","url":null,"abstract":"Cancer is becoming the main public health problem globally. Conventional chemotherapy approaches are slowly being replaced or complemented by new therapies that avoid the loss of healthy tissue, limit off-targets, and eradicate cancer cells. Immunotherapy is nowadays an important strategy for cancer treatment, that uses the host's anti-tumor response by activating the immune system and increasing the effector cell number, while, minimizing cancer's immune-suppressor mechanisms. Its efficacy is still limited by poor therapeutic targeting, low immunogenicity, antigen presentation deficiency, impaired T-cell trafficking and infiltration, heterogeneous microenvironment, multiple immune checkpoints and unwanted side effects, which could benefit from improved delivery systems, able to release immunotherapeutic agents to tumor microenvironment and immune cells. Nanoparticles (NPs) for immunotherapy (Nano-IT), have a huge potential to solve these limitations. Natural and/or synthetic, targeted and/or stimuli-responsive nanoparticles can be used to deliver immunotherapeutic agents in their native conformations to the site of interest to enhance their antitumor activity. They can also be used as co-adjuvants that enhance the activity of IT effector cells. These nanoparticles can be engineered in the natural context of cell-derived extracellular vesicles (EVs) or exosomes or can be fully synthetic. In this review, a detailed SWOT analysis is done through the comparison of engineered-synthetic and naturaly-derived nanoparticles in terms of their current and future use in cancer immunotherapy.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 23","pages":" 5933-5960"},"PeriodicalIF":5.8,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0