The regenerative capacity of cell imprinting and collagen/PCL scaffolds in gastrocnemius tendon defect.

IF 5.8 3区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS
Seyed Aliakbar Hosseini Toopghara, Shahin Bonakdar, Sara Nayyeri, Morteza Mehrjoo, Fatemeh Ale Ebrahim, Hossein Aminianfar, Farzad Mohammadian Sabour, Leila Montazeri, Mohammad Amin Hajari, Mohammad Ali Shokrgozar, Sang-Won Park, Mohammad Mehdi Dehghan
{"title":"The regenerative capacity of cell imprinting and collagen/PCL scaffolds in gastrocnemius tendon defect.","authors":"Seyed Aliakbar Hosseini Toopghara, Shahin Bonakdar, Sara Nayyeri, Morteza Mehrjoo, Fatemeh Ale Ebrahim, Hossein Aminianfar, Farzad Mohammadian Sabour, Leila Montazeri, Mohammad Amin Hajari, Mohammad Ali Shokrgozar, Sang-Won Park, Mohammad Mehdi Dehghan","doi":"10.1039/d5bm00534e","DOIUrl":null,"url":null,"abstract":"<p><p>Traumatic tendon injuries are among the most common types of injuries, often characterized by insufficient and slow recovery. The current study aims to evaluate the regenerative capacity of a tissue-engineered tendon graft in a rabbit gastrocnemius tendon defect. This graft comprises gap-electrospun collagen-coated parallel polycaprolactone (PCL) fibers seeded with adipose-derived stem cells (ADSCs), which promoted to adopt a tenogenic phenotype using a tenocyte-imprinted substrate for the first time. Scanning electron microscopy (SEM) images confirmed the parallel structure and successful cell attachment to the scaffold. Sirius red staining, high-performance X-ray photoelectron spectroscopy, and water contact angle showed that collagen successfully coated and changed the surface hydrophilicity of the scaffold. Imprinted substrates showed tenocyte patterns in SEM images. In an <i>in vitro</i> evaluation, ICC and real-time polymerase chain reaction confirmed that the stem cells acquired tenogenic traits. In addition, histopathology scoring outcomes showed significant improvement in the Pattern group and an almost 2.58 times increase in the total score average compared to the control group. <i>In vitro</i> and <i>in vivo</i> therapy results show that differentiated ADSCs seeded on the collagen-coated PCL scaffold for tendon repair have astounding therapeutic potential.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomaterials Science","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1039/d5bm00534e","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0

Abstract

Traumatic tendon injuries are among the most common types of injuries, often characterized by insufficient and slow recovery. The current study aims to evaluate the regenerative capacity of a tissue-engineered tendon graft in a rabbit gastrocnemius tendon defect. This graft comprises gap-electrospun collagen-coated parallel polycaprolactone (PCL) fibers seeded with adipose-derived stem cells (ADSCs), which promoted to adopt a tenogenic phenotype using a tenocyte-imprinted substrate for the first time. Scanning electron microscopy (SEM) images confirmed the parallel structure and successful cell attachment to the scaffold. Sirius red staining, high-performance X-ray photoelectron spectroscopy, and water contact angle showed that collagen successfully coated and changed the surface hydrophilicity of the scaffold. Imprinted substrates showed tenocyte patterns in SEM images. In an in vitro evaluation, ICC and real-time polymerase chain reaction confirmed that the stem cells acquired tenogenic traits. In addition, histopathology scoring outcomes showed significant improvement in the Pattern group and an almost 2.58 times increase in the total score average compared to the control group. In vitro and in vivo therapy results show that differentiated ADSCs seeded on the collagen-coated PCL scaffold for tendon repair have astounding therapeutic potential.

细胞印迹和胶原/PCL支架修复腓肠肌肌腱缺损的再生能力。
外伤性肌腱损伤是最常见的损伤类型之一,通常以恢复不足和缓慢为特征。目前的研究旨在评估组织工程肌腱移植物在兔腓肠肌肌腱缺损中的再生能力。该移植物由间隙电纺丝胶原包被的平行聚己内酯(PCL)纤维和脂肪来源的干细胞(ADSCs)组成,首次使用腱细胞印迹底物促进其采用腱细胞表型。扫描电镜(SEM)图像证实了支架的平行结构和成功的细胞附着。天狼星红染色、高性能x射线光电子能谱和水接触角表明,胶原蛋白成功包被并改变了支架的表面亲水性。印迹底物在扫描电镜图像中显示出小细胞模式。在体外评估中,ICC和实时聚合酶链反应证实了干细胞获得了成腱性状。此外,与对照组相比,Pattern组的组织病理学评分结果有显著改善,总平均评分几乎增加了2.58倍。体外和体内治疗结果表明,分化的ADSCs植入胶原包被PCL支架用于肌腱修复具有惊人的治疗潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Biomaterials Science
Biomaterials Science MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
11.50
自引率
4.50%
发文量
556
期刊介绍: Biomaterials Science is an international high impact journal exploring the science of biomaterials and their translation towards clinical use. Its scope encompasses new concepts in biomaterials design, studies into the interaction of biomaterials with the body, and the use of materials to answer fundamental biological questions.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信