Biomaterials Science最新文献

{"title":"Multifunctional biomimetic liposomal nucleic acid scavengers inhibit the growth and metastasis of breast cancer†","authors":"Yuhang Miao, Kaizhen Wang, Xin Liu, Xin Wang, Yanwei Hu, Zhenwei Yuan and Dawei Deng","doi":"10.1039/D4BM01721H","DOIUrl":"10.1039/D4BM01721H","url":null,"abstract":"<p >Chemotherapy and surgery, though effective in cancer treatment, trigger the release of nucleic acid-containing pro-inflammatory compounds from damaged tumor cells, known as nucleic acid-associated damage-associated molecular patterns (NA-DAMPs). This inflammation promotes tumor metastasis, and currently, no effective treatment exists for this treatment-induced inflammation and subsequent tumor metastasis. To address this challenge, we developed a biomimetic liposome complex (Lipo-Rh<small>₂</small>) incorporating a hybrid structure of liposomes and dendritic polymers, mimicking cell membrane morphology. Lipo-Rh<small>₂</small> leverages the multivalent surface properties of dendritic polymers to clear cell-free nucleic acids while serving as both a structural stabilizer and targeting ligand <em>via</em> embedded ginsenoside Rh<small>₂</small>. Experimental data show that Lipo-Rh<small>₂</small> effectively reduces free nucleic acids in mouse serum through charge interactions, downregulates Toll-like receptor expression, decreases inflammatory cytokine secretion, and inhibits both primary tumor growth and metastasis. Compared to the current nucleic acid scavenger PAMAM-G3, Lipo-Rh<small>₂</small> demonstrates stronger antitumor effects, lower toxicity, and enhanced targeting capabilities. This biomimetic liposome-based nucleic acid scavenger represents a novel approach to nucleic acid clearance, expanding the framework for designing effective therapeutic agents.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 9","pages":" 2475-2488"},"PeriodicalIF":5.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrical hydrogel: electrophysiological-based strategy for wound healing","authors":"Xingan Qiu, Feng Xiang, Hong Liu, Fangbiao Zhan, Xuezhe Liu, Pengzhen Bu, Bikun Zhou, Qiaojian Duan, Ming Ji and Qian Feng","doi":"10.1039/D4BM01734J","DOIUrl":"10.1039/D4BM01734J","url":null,"abstract":"<p >Wound healing remains a significant challenge in clinical practice, driving ongoing exploration of innovative therapeutic approaches. In recent years, electrophysiological-based wound healing strategies have gained considerable attention. Specifically, electrical hydrogels combine the synergistic effects of electrical stimulation and hydrogel properties, offering a range of functional benefits for wound healing, including antibacterial activity, real-time wound monitoring, controlled drug release, and electrical treatment. Despite significant progress made in electrical hydrogel research for wound healing, there is a lack of comprehensive, systematic reviews summarizing this field. In this review, we survey the latest advancements in electrical hydrogel technology. After analyzing the mechanisms of electrical stimulation in promoting wound healing, we establish a novel classification framework for electrical hydrogels based on their operational principles. The review further provides an in-depth evaluation of the therapeutic efficacy of these hydrogels in various types of wounds. Finally, we propose future directions and challenges for the development of electrical hydrogels for wound healing.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 9","pages":" 2274-2296"},"PeriodicalIF":5.8,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polydopamine as a versatile optical indicator for colorimetric and fluorescence-based biosensing","authors":"Jena Subhra Sulipta, Haejin Jeong and Seonki Hong","doi":"10.1039/D5BM00084J","DOIUrl":"10.1039/D5BM00084J","url":null,"abstract":"<p >Beyond their well-established adhesive properties, polydopamine (pDA) and pDA-like materials are emerging as superior alternatives to conventional optical indicators in biosensing applications due to their exceptional biocompatibility, tunable optical properties, and high sensitivity, arising from their eumelanin-like physicochemical characteristics. These materials attract significant attention for their ability to function as optical probes and transducers, enabling precise and sensitive detection in complex biological environments. This review highlights recent advancements in developing pDA-based optical probes, emphasizing strategies for fine-tuning synthetic parameters to optimize material properties, clarifying the fundamental sensing mechanisms underlying pDA-based systems, and exploring their potential roles in addressing global healthcare challenges. By facilitating early disease detection, real-time monitoring, and targeted therapeutic intervention, pDA-based optical probes offer transformative solutions to pressing biomedical needs. Through a comprehensive examination of cutting-edge research, this review aims to illuminate how the unique attributes of pDA materials drive innovation in biosensing technologies and contribute to improved healthcare outcomes.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 9","pages":" 2261-2273"},"PeriodicalIF":5.8,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d5bm00084j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143661629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Localized and sustained delivery of indomethacin using poly(lactic-co-glycolic acid)-based microspheres to prevent traumatic heterotopic ossification†","authors":"Junmin Shen, Ti Zhang, Junyu Chen, Feifan Chang, Liming Yang, Zhikang Guo, Ruijing Chen, Penghao Liu, Ni Jiang and Yajun Xie","doi":"10.1039/D4BM01719F","DOIUrl":"10.1039/D4BM01719F","url":null,"abstract":"<p >Traumatic heterotopic ossification (THO) is a pathological condition characterized by abnormal bone formation in non-skeletal tissues, commonly occurring after surgery or trauma. Current treatments, including non-steroidal anti-inflammatory drugs and surgery, are often hindered by adverse effects and high recurrence rates. In this study, we explore the potential of indomethacin-loaded PLGA microspheres (INDO-PLGA MPs) as a targeted therapeutic strategy for THO prevention. Through emulsifying solvent volatilization, sustained release of INDO was effectively achieved, with the 30% drug loading exhibiting optimal encapsulation efficiency and an ideal release profile. Cellular and animal experiments further confirm the excellent biocompatibility of INDO-PLGA MPs. <em>In vitro</em> and <em>in vivo</em> analyses revealed that these microspheres effectively inhibited osteogenic and chondrogenic differentiation, which are critical pathways driving the progression of THO. Notably, the 30% INDO-PLGA MPs exhibited optimal efficacy in reducing ectopic bone volume and delaying THO onset in a tenotomy and burn rat model. Collectively, our findings highlight the promising potential of PLGA microspheres in enhancing the localized and sustained delivery of INDO, providing effective prevention of THO and offering a transformative approach to its management.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 9","pages":" 2381-2393"},"PeriodicalIF":5.8,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor signal amplification and immune decoy strategy using bacterial membrane-coated nanoparticles for immunotherapy†","authors":"Yifan Li, Weiwei Wang, Jiale Xu, Bei Zhao, Longying Xiong, Dan Ge, Yanping Wu, Xiaotan Dou, Yuping Fu, Lei Wang, Cheng Zhao and Min Chen","doi":"10.1039/D4BM01535E","DOIUrl":"10.1039/D4BM01535E","url":null,"abstract":"<p >In cancer therapy, tumor cells can diminish their signals through mechanisms such as immune escape, thereby evading recognition and elimination by the immune system. Providing tumor signals to enhance the recognition of tumor sites is considered a crucial approach in cancer treatment. Inspired by the decoy-induced directed feeding of fish, we propose a biomimetic nanoparticle system for tumor signal amplification. This biomimetic system comprises magnetically responsive nanoparticles and immune-inducing bacterial membranes. These designs work together to create a baiting effect at the tumor site, attracting and activating immune cells to attack. It has been demonstrated that the generated nanoparticles have the potential to be targeted and delivered to the tumor site under the influence of an external magnetic field, as demonstrated in preliminary <em>in vitro</em> and <em>in vivo</em> studies. Moreover, the nanoparticles utilize the bacterial membrane and cell membrane-translocated calreticulin to induce an immune response, simulating a decoy mechanism to recruit immune cells. The nanoparticles were proved to be effective in recruiting macrophages and neutrophils and reducing tumor size in animal experiments. These features make the nanoparticles an ideal candidate for treating tumors.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 9","pages":" 2368-2380"},"PeriodicalIF":5.8,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OligoA-tailed DNA for dense functionalization of gold nanoparticles and nanorods in minutes without thiol-modification: unlocking cross-disciplinary applications†","authors":"Feng Liu and Guoqing Wang","doi":"10.1039/D5BM00022J","DOIUrl":"10.1039/D5BM00022J","url":null,"abstract":"<p >DNA-functionalized gold nanoparticles (DNA–AuNPs) and nanorods (DNA–AuNRs) have emerged as key yet versatile biomaterials for applications in biosensing, diagnostics and programmable assembly. The high cost and sometimes complex procedures of functionalization of DNA onto AuNPs and AuNRs <em>via</em> the Au−thiol interaction may have set a threshold for its expanded application by researchers of diverse fields. Although oligoA-tailed DNA has been introduced as an alternative to thiolated DNA, its extended use has been largely confined to spherical nanoparticles with suboptimal functionalization density. Here we show a rapid and efficient method for high-density functionalization of both AuNPs and AuNRs using oligoA-tailed DNA <em>via</em> butanol dehydration, with the length of oligoA as short as A<small>₂</small>. By preventing secondary structure formation at an elevated temperature, our results demonstrate significantly enhanced DNA adsorption, further allowing for functionalization of a random sequence onto the AuNPs. This yields stable DNA–nanoparticle conjugates with superior stability and durability, suitable for <em>in situ</em> naked-eye loop-mediated isothermal amplification (LAMP) assay of bacterial pathogens and stimuli-responsive self-assembly. This study overcomes long-standing barriers in rapid, simple and low-cost preparation of DNA–AuNPs and DNA–AuNRs, paving the way for cross-disciplinary applications in diverse fields that were previously siloed and beyond.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 9","pages":" 2503-2513"},"PeriodicalIF":5.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metal–organic framework MIL-101(Fe) functionalized with folic acid as a multifunctional nanocarrier for targeted chemotherapy–photodynamic therapy†","authors":"Eman Serag, Esmail M. El-Fakharany, Sherif F. Hammad and Mohamed E. El-Khouly","doi":"10.1039/D4BM01738B","DOIUrl":"10.1039/D4BM01738B","url":null,"abstract":"<p >A novel folic acid-conjugated, iron-based MOF (MIL-101(Fe)) loaded with 1,8-acridinediones (DO8) was developed for targeted photodynamic therapy (PDT) of HepG-2 cells. This composite aims to trigger an anticancer response through sequential PDT and chemotherapy. The nanocomposite exhibited high stability in a physiological environment with a pH of 7.4. It was also able to release DO8 continuously in an acidic environment with a pH of 5, which shows that it can adapt to the conditions in the tumor microenvironment. The MIL-101(Fe)MOF-FA@DO8 nanoparticles (NPs) with 30% and 50% DO8 have been studied <em>in vitro</em> under different conditions (light and dark) and have been shown to be compatible with living tissues and specifically target HepG-2 cells. The IC<small>₅₀</small> values of 50% DO8 and 30% DO8 loaded MOF-FA were found to be 88.67 and 105.9 μg mL<small>⁻¹</small> under dark conditions, respectively. Under light conditions, they demonstrated the highest efficacy in inhibiting tumor cell growth. The IC<small>₅₀</small> values were found to be 8.94 and 11.78 μg mL<small>⁻¹</small>. Flow cytometry analysis of annexin V/PI-stained apoptotic and necrotic cells in HepG-2 cells treated with the modified MIL-101-FA@50% DO8 NPs at IC<small>₅₀</small> doses under both dark and light conditions indicates that the primary mechanism of cell death is necrosis, likely due to the enhanced formation of reactive oxygen species (ROS) under light conditions compared to that under dark conditions. This increased reactive oxygen species (ROS) generation leads to extensive membrane rupture, resulting in significant cell damage after treatment with the modified MIL-101-FA@50% DO8 NPs. These findings underscore the potential of this nanocomposite as an effective PDT agent for targeted cancer therapy.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 9","pages":" 2351-2367"},"PeriodicalIF":5.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-targeted nanosystem for enhanced chemodynamic cancer therapy†","authors":"Kanwal Asif, Md. Mahbubur Rahman, Vincenzo Canzonieri, Isabella Caligiuri, Flavio Rizzolio and Muhammad Adeel","doi":"10.1039/D4BM01683A","DOIUrl":"10.1039/D4BM01683A","url":null,"abstract":"<p >Chemodynamic therapy (CDT) could have a significant potential for advancing cancer treatment <em>via</em> the utilization of Fenton and Fenton-like reactions, which produce toxic reactive species. Nonetheless, the efficacy of CDT is constrained by the limited availability of catalyst ions capable of decomposing pre-existing intracellular H<small>₂</small>O<small>₂</small> and generating reactive oxygen species (ROS) necessary to achieve a therapeutic response. To address these limitations, a tailored strategy has been developed to enhance the efficacy of Fenton-like reactions to eradicate selectively cancer cells. This innovative approach involves the utilization of dual metal cations (Zn<small>²⁺</small>, Fe<small>²⁺</small>) within zinc nitroprusside (ZnNP) material. Remarkably, this method takes advantage of the acidic conditions prevalent in tumors, thus eliminating the need for external stimuli. Through these advancements, the tailored approach exhibits the potential to specifically target and eliminate cancer cells, overcoming the mentioned limitations. A simple mixing technique was utilized to synthesize ZnNP, which was structurally and morphologically characterized. Furthermore, extensive <em>in vitro</em> investigations were conducted to assess its anti-tumoral mechanism of action. ZnNP exhibits a remarkable capability to increase intracellular H<small>₂</small>O<small>₂</small> within cells. This process leads to the generation of various reactive species, including hydroxyl (˙OH) and superoxide (O<small>₂</small>˙<small>⁻</small>) radicals, and peroxynitrite (ONOO<small>⁻</small>), which act as apoptotic inducers specifically targeting cancer cells. Cellular uptake studies have shown that ZnNP enters the lysosomes, evades degradation, and takes advantage of their acidic pH environment to significantly increase the production of ROS. These findings are further supported by the activation of multiple oxidative genes. Furthermore, the biocompatibility of ZnNP has been demonstrated in <em>ex vivo</em> models using healthy liver cells. Notably, ZnNP exhibited therapeutic effectiveness in high-grade serous ovarian cancer (HGSOC) patient-derived tumor organoids (PDTO), further confirming its potential as a therapeutic agent. The present study highlights the therapeutic potential of ZnNP as a generator of multiple ROS <em>via</em> a Fenton-like reaction. This research offers a promising therapeutic approach for CDT application in combatting HGSOC, a highly aggressive and life-threatening cancer.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 9","pages":" 2320-2331"},"PeriodicalIF":5.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d4bm01683a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthetic hydrogels support robust and reproducible cardiomyocyte differentiation†","authors":"Margot J. Amitrano, Mina Cho, Eva M. Coughlin, Sean P. Palecek and William L. Murphy","doi":"10.1039/D4BM01636J","DOIUrl":"10.1039/D4BM01636J","url":null,"abstract":"<p >Cardiomyocyte manufacturing from human pluripotent stem cells is limited by the variability of differentiation efficiencies, partly attributed to the widespread use of the tumor-derived substrate Matrigel. Here, we describe a screening approach to identify fully-defined synthetic PEG hydrogels that support iPSC-derived cardiac progenitor cell (iPSC-CPC) adhesion, survival, and differentiation into iPSC-derived cardiomyocytes (iPSC-CMs). Our PEG hydrogels supported superior iPSC-CM differentiation efficiency, with a 24% increase in cTnT expression, and greater reproducibility when compared to cells cultured on Matrigel. By combining our 5-level, 3-variable full factorial screening approach with multi-variate analysis, we showed that all substrate variables manipulated here (adhesion ligand type/concentration, stiffness) had a significant influence on iPSC-CPC confluency and that iPSC-CM differentiation was significantly influenced by adhesion ligands. These results highlight the benefit of synthetic, tunable cell culture substrates and multi-variate screening studies to identify substrate formulations for a targeted cell behavior.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 8","pages":" 2142-2151"},"PeriodicalIF":5.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antitumor immune response elicited by M2 TAM-specific DDS via C-type lectin CD209b using cholesteryl pullulan nanogel as a protein drug carrier†","authors":"Takaaki Nakatsukasa, Daisuke Muraoka, Situo Deng, Kiyoshi Yasui, Shin-ichi Sawada, Asako Shimoda, Hirokazu Matsushita, Keitaro Matsumoto, Takeshi Nagayasu, Naozumi Harada, Kazunari Akiyoshi and Hiroaki Ikeda","doi":"10.1039/D5BM00342C","DOIUrl":"10.1039/D5BM00342C","url":null,"abstract":"<p >Many cancer patients develop resistance to immunotherapy, highlighting the urgent need for novel therapeutic strategies. Various factors contribute to tumor resistance to immunotherapy, among which tumor-associated macrophages (TAMs) are critical regulators of tumor sensitivity. Therefore, combining cancer immunotherapies with drug delivery systems (DDSs) targeting TAMs has become an intriguing treatment strategy. However, the target molecules used in DDSs are limited to a few receptors expressed on TAMs. Therefore, the identification of novel target molecules for TAM-specific DDS is urgently needed. The current study evaluated the ability of a cholesteryl pullulan (CHP) nanogel to target TAMs <em>via</em> mDC-SIGN (CD209b). This nanogel encapsulated the cytotoxic protein drug Pseudomonas exotoxin A and was injected into a tumor-bearing mouse model. This treatment significantly reduced the abundance of CD209b-positive M2 TAMs and enhanced antitumor immune responses. Ultimately, tumor growth was suppressed, even in a low-immunogenic tumor model. Hence, CD209b is an effective target molecule for M2 TAM-specific DDSs that can be used to develop novel cancer therapies.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 9","pages":" 2340-2350"},"PeriodicalIF":5.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}