Biomaterials Science最新文献

{"title":"Functionalized transdermal collagen mimetic peptides for efficient repair of subacute aged skin.","authors":"Jingting Zhang, Guangyu Liu, Yi Yang, Yi Xie, Linyan Yao, Jianxi Xiao","doi":"10.1039/d5bm00940e","DOIUrl":"10.1039/d5bm00940e","url":null,"abstract":"<p><p>Skin aging compromises both aesthetic appearance and barrier function, presenting significant challenges for dermal regeneration. Collagen represents a crucial target for anti-aging interventions; however, they suffer from the risk of virus transmission, large molecular weight and difficulty in transdermal transmission. Herein, we designed a series of transdermal collagen mimetic peptides (TSTPs) and a functionalized transdermal collagen mimetic peptide for efficient repair of subacute aged skin. TSTP-2, with the sequence (GPO)₈KLPVM, was identified as the optimal peptide according to its superior biocompatibility and skin permeability. Mechanistic analysis indicated that TSTP-2 improved transdermal delivery by perturbing skin lipids, engaging with keratin proteins, and altering hydration dynamics. The functionalized transdermal collagen mimetic peptide (TNC), engineered through GFOGER incorporation into TSTP-2, exhibited a stable triple-helical structure, effective transdermal delivery and high biological activity, significantly promoting fibroblast proliferation, adhesion, migration, and myofibroblast differentiation. The results of the DermaLab® Combo skin analyzer and histological analysis demonstrated that TNC promoted subacute aged skin repair by increasing epidermal thickness, stimulating collagen and elastic fiber regeneration, improving hydration, and reducing oxidative stress. The use of functionalized transdermal collagen mimetic peptides represents an alternative strategy for collagen transdermal delivery, showing potential applications in skin rejuvenation and regenerative medicine.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MOF-based nanoagent for self-illuminating photodynamic therapy.","authors":"Jing-Lan Kan, Fan-Hong Lv, Wen-Xiu Ren, Qun Guan, Anqi Xue, Jie Feng, Yu-Bin Dong","doi":"10.1039/d5bm00963d","DOIUrl":"10.1039/d5bm00963d","url":null,"abstract":"<p><p>Photodynamic therapy (PDT) is a minimally invasive and local light-activated treatment for annihilating tumor tissues. However, it is often limited by the tissue penetration depth of common external light sources. To address this issue, we herein report a self-illuminating ZIF-90 MOF-based composite photosensitive system prepared using a combined one-pot self-assembly and post-synthetic modification (PSM) methodology. The obtained PME@Zn/Fe-ZIF-90-Lum consists of the Fe(II)-doped and luminol-decorated MOF host as well as the protoporphyrin IX dimethyl ester (PME) guest. Under the given conditions, the light, which is generated from the Fe(II)-catalysed luminol oxidation by endogenous H₂O₂, can <i>in situ</i> activate the encapsulated photosensitizer PME to produce reactive oxygen species (ROS) <i>via</i> a chemiluminescence resonance energy transfer (CRET) process. In the absence of an external light source, antitumor PDT is successfully realized and high phototoxicity is fully evidenced by <i>in vitro</i> and <i>in vivo</i> experiments.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthetic biology-driven design and bioproduction of mechanical proteins","authors":"Yuqing Wang, Fan Wang, Huijing Cui, Jingjing Li, Hongjie Zhang and Kai Liu","doi":"10.1039/D5BM00646E","DOIUrl":"10.1039/D5BM00646E","url":null,"abstract":"<p >Structural proteins, with exceptional structural versatility and tailored properties, have inspired the design of mechanically robust biomaterials with diverse applications. Nevertheless, the heterologous biosynthesis of high-performance structural proteins faces inherent challenges due to their characteristic high molecular weight, highly repetitive sequences, and intricate folding patterns, frequently leading to reduced production yields, structural defects, and compromised mechanical properties. To overcome these limitations, researchers have adopted various strategies to refine sequence architectures and optimize heterologous expression systems of recombinant structural proteins. This review comprehensively summarizes recent advances in biosynthetic engineering for the heterologous production of structural proteins, highlighting key modifications in expression hosts, innovative molecular design approaches, and optimized cultivation conditions. These methodologies provide valuable insights for enhancing the yield and stability of high-molecular-weight recombinant structural proteins. Additionally, by evaluating current challenges and future directions in structural protein design and biosynthesis, this review seeks to further innovation in the development of advanced structural protein-based biomaterials.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 18","pages":" 4970-4983"},"PeriodicalIF":5.7,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune modulation strategies to reduce in-stent restenosis","authors":"Mei Yu, Chengyi Dai, Jingjing Shi and Jiayin Fu","doi":"10.1039/D5BM00687B","DOIUrl":"10.1039/D5BM00687B","url":null,"abstract":"<p >In-stent restenosis remains a significant complication following stent implantation, driven by complex interactions between immune responses, vascular injury, and inflammatory cascades. Despite advancements in stent technology, ISR persists, underscoring the need for innovative strategies to modulate immune activity and promote vascular healing. This review presents current knowledge on immune-mediated mechanisms of ISR, highlighting the pivotal roles of immune cell like neutrophils, macrophages in neointimal hyperplasia and chronic inflammation. We explore recent immunomodulatory approaches, including stent surface modifications, bioactive molecule delivery, and emerging technologies. Furthermore, we evaluate the clinical potential of next-generation stents such as endothelial-mimetic designs to mitigate ISR by balancing pro-reparative and anti-inflammatory signals. By integrating insights from preclinical and clinical studies, this review provides a new perspective for developing “immune-friendly” stents, emphasizing interdisciplinary strategies to attenuate ISR following stent implantation.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 18","pages":" 4898-4915"},"PeriodicalIF":5.7,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144774350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of concern: The effect of metal ions on endogenous melanin nanoparticles used as magnetic resonance imaging contrast agents","authors":"Anqi Chen, Jinghua Sun, Shijie Liu, Liping Li, Xiaoyang Peng, Lixin Ma and Ruiping Zhang","doi":"10.1039/D5BM90061A","DOIUrl":"10.1039/D5BM90061A","url":null,"abstract":"<p >Expression of Concern for ‘The effect of metal ions on endogenous melanin nanoparticles used as magnetic resonance imaging contrast agents’ by Anqi Chen <em>et al.</em>, <em>Biomater. Sci.</em>, 2020, <strong>8</strong>, 379–390, https://doi.org/10.1039/C9BM01580A.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 18","pages":" 5215-5217"},"PeriodicalIF":5.7,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d5bm90061a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144774349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the impact of CAP-p15 functionalization on the bioactivity of rough TiO2-coated 316L stainless steel surfaces","authors":"Guadalupe Ureiro-Cueto, Sandra E. Rodil, José Ocotlán Flores-Flores, Lía Hoz-Rodríguez, Higinio Arzate and Gonzalo Montoya-Ayala","doi":"10.1039/D5BM00294J","DOIUrl":"10.1039/D5BM00294J","url":null,"abstract":"<p >Stainless steel 316L (316L SS) is frequently used in implants and medical devices because of its low cost, high mechanical strength, and adequate biocompatibility. However, its bioinert nature limits osseointegration, often confining its applications to temporary uses. To address this issue, surface modifications such as oxide coatings and peptide adsorption have emerged as promising strategies to enhance the bioactivity of 316L SS. This study explores the surface modification of 316L SS substrates through sandblasting, followed by the deposition of a TiO<small>₂</small> layer and subsequent biofunctionalization with a cementum attachment protein-derived peptide (CAP-p15) <em>via</em> physisorption using three different concentrations. The modified surfaces were characterized using X-ray photoelectron spectroscopy (XPS), scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (SEM/EDX), surface roughness analysis, and water contact angle measurements (WCA). Samples were incubated in artificial saliva (AS) for 21 days. The resulting peptide release, surface microstructure, the morphology and chemical composition of the deposits were evaluated. Additionally, human periodontal ligament cells (hPDLCs) were cultured on the modified surfaces to assess cell viability and attachment. Characterization revealed significant changes in surface chemistry, roughness, and wettability following functionalization. <em>In vitro</em> testing in AS demonstrated the formation of carbonated apatite, indicative of enhanced bioactivity. Furthermore, hPDLCs cultured on functionalized surfaces exhibited enhanced viability, improved adhesion, and enhanced cell spreading. These results suggest that peptide-based functionalization with CAP-p15 is a promising strategy for enhancing the osseointegration potential of 316L SS, offering valuable prospects for bone tissue regeneration.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 18","pages":" 5122-5133"},"PeriodicalIF":5.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d5bm00294j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modified chitosan-fabricated antifungal transdermal array for dermatophytosis therapy","authors":"Feng Feng, Xing Ge, Tianye Fang, Kaiyang Chen, Jiaying Zhu, Jun Yang, Lei Tao, Jianjun Dai and Yanmin Ju","doi":"10.1039/D5BM00844A","DOIUrl":"10.1039/D5BM00844A","url":null,"abstract":"<p >Dermatophytosis is a complex and prevalent fungal skin infection that affects a significant portion of the global population. Conventional antifungal therapies are often compromised by their inability to effectively penetrate fungal biofilms and limited skin permeability, ultimately leading to unsatisfactory clinical results. To address these issues, we proposed a novel dissolvable microneedle (MN) patch (denoted as TB/QCS MNs) that encapsulated terbinafine (TB) within the quaternary ammonium chitosan (QCS) MN tips. The combination of QCS and TB exhibited a pronounced synergistic effect against <em>Trichophyton mentagrophytes</em> (<em>T. mentagrophytes</em>). Importantly, TB/QCS MNs effectively disrupted fungal biofilms and damaged extracellular polymeric substances. In comparison to TB alone, TB/QCS MNs effectively accelerated fungal clearance, shortened treatment time, and enhanced skin recovery in <em>T. mentagrophytes</em>-infected C57BL/6 mice. These findings demonstrated that TB/QCS MNs significantly improve therapeutic efficacy for dermatophytosis, offering a promising alternative for clinical treatment of skin fungal infections.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 18","pages":" 5111-5121"},"PeriodicalIF":5.7,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of hair follicle spheroids with growth factor transfection and various cell combinations","authors":"Hyeon-Min Nam, Subin Jeong and Gun Yong Sung","doi":"10.1039/D5BM00998G","DOIUrl":"10.1039/D5BM00998G","url":null,"abstract":"<p >As the number of hair loss patients increases, research conducted on hair follicles and hair has recently become more active. However, most current hair follicle research models are limited in their ability to reproduce several key functions of the hair follicle microenvironment. To solve this problem, hair growth genes were injected into hair follicle cells, and long-term culture was performed for up to 90 days. DPCs (dermal papilla cells), KCs (keratinocytes), HUVECs (human umbilical vein endothelial cells) or HDMECs (human dermal microvascular endothelial cells), and FBs (fibroblasts) injected with hair follicle growth factors LEF1 and Wnt1 were mixed to produce spheroids, and the growth factor expression and growth behavior according to the type of cell and the presence or absence of transfection according to the culture period were analyzed using SEM, H&amp;E staining, and IF imaging. As the culture period increased, the size of the spheroids increased, and the number decreased due to the association between spheroids; long fibers that appeared to be hair expression were observed. We also compared the differences between HUVECs and HDMECs, which have different phenotypes of vascular cells. In spheroids that did not contain FBs, the expression of HUVECs was more promoted, whereas in spheroids that contained FBs, the expression of HDMECs tended to be more promoted. These results are expected to contribute to dermatology and new drug development for the prevention of intractable skin diseases and hair loss using skin models with a microbiome environment in the future.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 18","pages":" 5184-5201"},"PeriodicalIF":5.7,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144853966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction strategies of whole tumor cell vaccines","authors":"Xilin Wang, Hanye Xu, Hongyi Wang, Runjia Hua, Shuwang Wu, Jingwei Xu, Xiaju Cheng and Haibin Shi","doi":"10.1039/D5BM00914F","DOIUrl":"10.1039/D5BM00914F","url":null,"abstract":"<p >Whole tumor cell vaccines (WTCVs), which utilize the entire tumor antigen repertoire to elicit robust and personalized anti-tumor immunity, have emerged as a promising strategy to overcome tumor heterogeneity and immune evasion. However, the development of WTCVs has very limited benefits in the clinical setting. This paper systematically reviews the cutting-edge construction strategies of WTCVs including dead tumor cell-based vaccines and engineered living tumor cell-based vaccines with a particular focus on the impact of structural integrity on the immune response of WTCVs. Moreover, current challenges and prospects are also discussed, aiming to achieve better therapeutic efficacy by fabricating WTCVs with great biosafety and strong immunogenicity, hoping to promote the clinical translation of WTCVs.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 18","pages":" 4885-4897"},"PeriodicalIF":5.7,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144774348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymer-engineered PROTAC nanovehicles amplify synergistic effects with temozolomide by BRD4 degradation†","authors":"Yun Guo, Haoyu You, Yiyang Li, Zheng Zhou, Zonghua Tian, Chen Jiang and Tao Sun","doi":"10.1039/D5BM00443H","DOIUrl":"10.1039/D5BM00443H","url":null,"abstract":"<p >As the most aggressive primary brain tumor, glioblastoma (GBM) remains therapeutically challenging. Proteolysis-targeting chimeras (PROTACs), capable of degrading target proteins like BRD4, offer a promising strategy for GBM therapy. However, their clinical application is limited by poor solubility, stability, and bioavailability. This study systematically evaluates PLGA, PCL, and poly amino-acid based nanoparticles (NPs) for optimizing ARV-825, a BRD4-degrading PROTAC. This study compares the particle size, polydispersity index (PDI), and encapsulation efficiency of NPs prepared by different methods and carriers, explores the computer-simulated design of cyclic peptide carriers, and reveals the impact of PROTAC's molecular structure and action time on its toxicity. Furthermore, the delivery of ARV-825 using NPs achieves synergistic anti-tumor effects with temozolomide (TMZ) in GBM cells. These findings validate nanovehicles as a strategic solution for PROTAC limitations and provide a blueprint for translating catalytic degradation into clinically viable therapies against GBM.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 18","pages":" 5001-5016"},"PeriodicalIF":5.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}