Biomaterials Science最新文献

{"title":"Enzyme-mimicking catalytic activities and biomedical applications of bimetallic nanozymes.","authors":"Xiaoxiao Li, Guangrui Zhou, Shanwen Gong, Jiaqi Hao, Qingwang Xue, Qi Zhang","doi":"10.1039/d5bm01089f","DOIUrl":"https://doi.org/10.1039/d5bm01089f","url":null,"abstract":"<p><p>Bimetallic nanostructures, characterized by a structural complexity and hierarchy akin to natural metalloproteases, have garnered considerable interest in the field of artificial enzyme research. These protein-like structures impart bimetallic nanostructures with enzyme-like catalytic activities, encompassing peroxidase-, catalase-, and superoxide dismutase-mimicking activities. This suggests significant potential for application in biomedical domains. This review endeavors to synthesize bimetallic nanozymes, focusing on the hetero-metal spatial arrangement and elucidating the structural basis underlying their catalytic efficacy. The enzyme-like activities are systematically discussed. Typically, the catalytic mechanism of bimetallic nanozymes entails electronic structure modulation, interfacial synergy, and the convergence of multiple enzyme-like functions. By capitalizing on the synergistic interaction between the two metals, the active center structure and electron transfer mechanism akin to natural enzymes can be established, leading to highly efficient substrate conversion. Furthermore, beyond structure-property correlations, this review illustrates biomedical applications arising from the catalytic mimicry of bimetallic nanozymes, encompassing theranostics for wound healing, periodontitis, and oral infections, bone regeneration, tumor treatment, biosensing <i>etc.</i> The fundamental and methodological insights presented here will be instrumental in advancing the development of bimetallic nanozymes as a novel class of artificial enzymes.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145123838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional erythromycin-loaded liposomes: a methodological optimization for enhanced mucoadhesion, antioxidant activity, and biocompatibility.","authors":"Vera-Maria Platon, Anda Mihaela Craciun, Irina Rosca, Natalia Simionescu, Luminita Marin","doi":"10.1039/d5bm00629e","DOIUrl":"https://doi.org/10.1039/d5bm00629e","url":null,"abstract":"<p><p>In recent years, liposomes have emerged as versatile nanocarriers for the delivery of antibacterial agents, enhancing drug pharmacokinetics in an effort to overcome antibiotic resistance. This study presents a systematic, multivariate optimization of erythromycin-loaded liposomes (ERY-liposomes) coated with chitosan oligomers (CSO), aiming for drug encapsulation into nanoscale-sized particles, while promoting mucoadhesive, antioxidant, antimicrobial, and biocompatibility attributes. Critical formulation parameters including lipid-to-drug ratio, thin-film formation conditions, hydration medium and time, liposome downsizing technique, chitosan molecular weight and concentration, as well as cryoprotectant content were comprehensively optimized <i>via</i> multivariate analysis. Physicochemical and structural characterization was conducted using a broad array of techniques: FTIR, ¹H-NMR, DLS, STEM, AFM, XRD, and variable temperature polarized light microscopy (POM). The optimized ERY-liposomes achieved an encapsulation efficiency of 63%, a hydrodynamic diameter of 97 nm, and a low polydispersity index (PDI < 0.1), indicative of uniform size distribution. Structural analysis revealed strong intermolecular forces among ERY, CSO and the phospholipid, resulting in densely packed vesicles incorporating the drug in an amorphous state. STEM imaging displayed spherical morphology with compact cores surrounded by a rough coating, and POM indicated enhanced thermal stability. The formulation demonstrated sustained ERY release governed by diffusion and matrix erosion mechanisms, potent antibacterial activity over 24 hours, and considerable early bactericidal activity, particularly against Gram-positive strains. Additionally, ERY-liposomes displayed pronounced scavenging activity (80% radical inhibition, EC₅₀ = 0.396 mg mL^-1 ERY), and mucoadhesive properties, as well as cytocompatibility with normal human fibroblasts. These findings indicate the advanced therapeutic potential of ERY-liposomes.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145123845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retinal hypoxia reversal with PLGA-oxygen nanobubbles.","authors":"Anika Bushra, Wen Ren, Daniel Um, Xiaoxue Han, Michael Tsipursky, Joseph Irudayaraj","doi":"10.1039/d5bm01154j","DOIUrl":"https://doi.org/10.1039/d5bm01154j","url":null,"abstract":"<p><p>Pathologies associated with retinal hypoxia, including diabetic retinopathy, central/branch retinal artery occlusion (CRAO/BRAO), central/branch retinal vein occlusion (CRVO/BRVO), retinopathy of prematurity, sickle cell retinopathy, <i>etc</i>., have limited effective therapeutic intervention strategies. To address this shortcoming, herein we propose a biocompatible and biodegradable poly (lactic-<i>co</i>-glycolic acid) shell-based oxygen nanobubbles (PLGA-ONBs) platform, formulated with PLGA, polyvinyl alcohol (PVA), and NaHCO₃. The formulation of a novel PLGA-ONBs was proposed, and the synthesis process was optimized with respect to dependent (sonication power, PVA, and NaHCO₃ concentrations) and response (hydrodynamic diameter and oxygen capacity) variables. The optimized formulation has a concentration of (13.8 ± 0.01) × 10¹⁰ particles per ml with a hydrodynamic diameter of 142.83 ± 11.46 nm, and oxygen loading capacity of 47.2 ± 2.4 mg L^-1. After 4 weeks of storage, the ONBs were found to have an oxygen concentration of 38.9 ± 2.9 mg L^-1, indicating excellent oxygen retention capability. The PLGA-ONBs tested <i>in vitro</i> in Muller and R28 retinal cell lines demonstrated excellent biocompatibility and potential to mitigate hypoxia. In addition, the PLGA-ONBs treatment on hypoxic cells demonstrated restoration of mRNA expression of three key hypoxic genes (HIF-1α, PAI-1, and VEGF-A) to normoxic states, indicating hypoxia reversal potential. Biosafety of the PLGA-ONBs was demonstrated in a rabbit model, demonstrating promise in clinical translation. The PLGA-ONBs developed exhibited excellent oxygen loading and retention, potential in hypoxia mitigation, and a safety profile that could be a promising route to treating ischemic diseases of the eye.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145123774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microwave-triggered organic sensitizer for synergistic dynamic/thermal therapy of osteosarcoma.","authors":"Yue He, Man Shu, Guiquan Zhang, Ziyang Xu, Teliang Lu, Xiao Chu, Mei Li, Xiongfa Ji, Jielong Zhou, Qiao Zhou, Guoqing Zhong, Renshan Li, Jin Ke, Zhidan Tan, Anjun Qin, Yu Zhang","doi":"10.1039/d5bm00895f","DOIUrl":"https://doi.org/10.1039/d5bm00895f","url":null,"abstract":"<p><p>Osteosarcoma (OS) is an aggressive malignant tumor with high recurrence and metastatic potential, primarily affecting adolescents. Despite the standard clinical \"sandwich\" treatment regimen, patient survival rates have remained stagnant. Microwave ablation (MWA), known for its rapid heating and strong tissue penetration, has emerged as a viable therapeutic strategy for bone tumors. However, MWA poses challenges, particularly the potential for iatrogenic damage to adjacent healthy tissues and the concern that inappropriate energy delivery parameters may paradoxically stimulate neoplastic progression. In this study, microwave-triggered organic nanoparticles, termed ATT, were synthesized <i>via</i> a spontaneous [2 + 2] cycloaddition-cycloreversion (CA-CR) reaction. Findings from cell-based assays and animal studies indicated that ATT effectively generated reactive oxygen species (ROS) and produced heat under microwave (MW) irradiation, achieving a remarkable tumor inhibition rate of 95%. Owing to its unique donor-acceptor (D-A) structure and twisted molecular configuration, ATT facilitates efficient charge transfer under MW irradiation. The process generates electron-hole pairs, enhancing ROS production and enabling an effective microwave dynamic therapy (MDT) effect. Moreover, ATT acts as a polar molecule that efficiently absorbs MW and generates heat through resonance, thereby potentiating the therapeutic performance of microwave thermal therapy (MTT). This study presents a novel microwave-triggered organic nanomaterial that integrates MTT and MDT, offering a prospective therapeutic intervention for OS.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive coating with clindamycin, VEGF-165, and TGF-β1 for supporting bone tissue regeneration.","authors":"Dagmara Słota, Aleksandra Szwed-Georgiou, Marcin Włodarczyk, Agnieszka Krupa, Karolina Rudnicka, Karina Niziołek, Bartłomiej Kryszak, Konrad Szustakiewicz, Agnieszka Sobczak-Kupiec","doi":"10.1039/d5bm00637f","DOIUrl":"https://doi.org/10.1039/d5bm00637f","url":null,"abstract":"<p><p>The growing demand for implantable devices, implants, and plastic surgery is a major factor driving the growth of the global biomaterials market. Both new materials and opportunities to enhance the properties of existing solutions are being explored. One such approach involves coating existing materials with bioactive layers to provide additional functions. In this study, a bioactive coating was developed in an environmentally friendly and cost-effective manner, using polyethylene glycol (PEG), polyvinylpyrrolidone (PVP), hydroxyapatite (HAp), collagen (COL), and glutathione (GSH). The coating demonstrated the ability to release the antibiotic clindamycin, the vascular endothelial growth factor-165 (VEGF-165), which promotes angiogenesis, and the transforming growth factor-β1 (TGF-β1), which provides anti-inflammatory properties. The physicochemical properties of the coating were evaluated, and its <i>in vivo</i> integration with natural bone tissue was assessed using a rat skull bone defect model in adult Wistar rats (Rattus norvegicus). It was demonstrated that VEGF-165 and TGF-β1 were released within 24 hours at approximately 30% each, a dose capable of producing a therapeutic effect. The <i>in vivo</i> results suggest that incorporating growth factors into the composite coating significantly promotes mineralization at the site of injury. Our coating has the potential to support bone tissue regeneration through the synergistic effects of proteins; however, further studies are required.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the therapeutic potential of exosomes for ischemic stroke.","authors":"Meilin Shen, Yan Zhu, Qi Chen, Huanghao Yang","doi":"10.1039/d5bm01007a","DOIUrl":"https://doi.org/10.1039/d5bm01007a","url":null,"abstract":"<p><p>Ischemic stroke, a leading cause of global mortality and disability, faces therapeutic limitations due to the narrow time window and restricted efficacy of current interventions such as thrombolysis and thrombectomy. Recent advancements highlight exosomes-nanoscale extracellular vesicles-as promising therapeutic agents owing to their ability to cross the blood-brain barrier (BBB) and mediate intercellular communication. This review summarizes the biological characteristics of exosomes, their roles in neuroprotection, neuroregeneration, and angiogenesis following ischemic stroke, and emerging strategies utilizing engineered exosome-based nanoparticles for targeted therapy. Despite exosomes showing significant advantages in the treatment of ischemic stroke, their clinical transformation still faces challenges, including the standardized production of exosomes, the clarification of biological distribution mechanisms, and the assessment of immunogenicity and safety. Overcoming these challenges, exosomes are expected to become a safe and efficient therapeutic means for ischemic stroke.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptide-based supramolecular nanomaterials with super-large red-shifted absorption for antitumor therapy.","authors":"Yusong Wang, Haining Lu, Wen Xie, Lei Wang, Qianli Zou, Shaozhen Wang","doi":"10.1039/d5bm00945f","DOIUrl":"https://doi.org/10.1039/d5bm00945f","url":null,"abstract":"<p><p>Indocyanine green (ICG) is a near-infrared dye with photothermal therapy (PTT) and photodynamic therapy (PDT) effects, widely used in cancer treatment. In this study, a multifunctional nanoplatform (ICG-TP-5) was successfully constructed through the supramolecular co-assembly of ICG and TP-5, enabling multimodal synergistic therapy for tumors. The nanomaterial demonstrated remarkable stability in aqueous solution at 4 °C for at least three months, effectively addressing the inherent limitations of ICG while enhancing its bioavailability. Driven by molecular self-assembly-induced J-aggregation effects, the near-infrared (NIR) absorption peak of the material exhibited super-large red-shift absorption from 785 nm to 947 nm, significantly enhancing tissue penetration depth and photoconversion efficiency. This optical optimization synergistically amplified both PTT and PDT efficacy, offering a promising strategy for treating deep-seated malignancies. Innovatively, this platform integrated PDT/PTT with TP-5-mediated immune activation, establishing a trimodal therapeutic mechanism. <i>In vitro</i> experiments confirmed its selective cytotoxicity against diverse cancer cells, while <i>in vivo</i> studies revealed that the combined therapy markedly suppressed tumor growth and activated systemic antitumor immunity. These findings provide a robust nanomedicine candidate with enhanced stability, deep-tissue penetrability, and multimodal therapeutic synergy, paving the way for precision treatment of aggressive and deep-seated tumors.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogel design for intestinal organoids: principles governing translational regenerative medicine.","authors":"Yang Wang, Zhen Cao, Bo Li, Yin Huang, Guixue Wang, Qingmei Chen","doi":"10.1039/d5bm00926j","DOIUrl":"https://doi.org/10.1039/d5bm00926j","url":null,"abstract":"<p><p>Intestinal organoids (IOs) are self-organized tissue constructs, grown <i>in vitro</i>, that closely replicate the structural and functional characteristics of the intestinal epithelium. The advent of IOs has significantly advanced research in areas such as intestinal development, disease modeling, drug screening, personalized medicine, regenerative medicine, <i>etc</i>. The development and functional maturation of organoids <i>in vitro</i> is heavily reliant on the presence of an extracellular matrix with appropriate biophysical properties. The significant breakthrough of polymer hydrogels offers tunable biochemical and biophysical properties, enabling efficient and high-quality cultivation of organoids. In this review, we provide a comprehensive evaluation of IO culture systems and discuss how mechanobiological signaling dynamics at the cell-matrix interface can guide the rational engineering of biomimetic extracellular matrix to standardize and regulate IO culture phenotypes. We systematically classify hydrogel-based matrices encompassing natural and synthetic hydrogel. We focus on how hydrogel properties affect the growth and development of IOs. We highlight biomimetic hydrogel innovations for applications in disease modeling, drug screening, regenerative medicine, and therapeutic delivery. By summarizing the challenges that hinder the development of IO hydrogels, we aim to provide insights into future directions for their advancement and promote the broader application of IOs in biomedicine.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Octahedral iodide Mo₆ cluster complex bearing thiosulfate ligands: a dual chemotherapeutic and radiodynamic agent for advanced cancer therapy.","authors":"Tatiana N Pozmogova, Margarita V Vegner, Yuri A Vorotinkov, Sofia V Korotkova, Mariya A Gromova, Alphiya R Tsygankova, Tatiana Ya Guselnikova, Georgy D Vavilov, Natalia V Kuratieva, Dmitri V Stass, Lidiya V Shestopalova, Olga P Khripko, Alexander M Shestopalov, Michael A Shestopalov","doi":"10.1039/d5bm00909j","DOIUrl":"https://doi.org/10.1039/d5bm00909j","url":null,"abstract":"<p><p>Cancer treatment faces significant challenges due to tumor heterogeneity, drug resistance, and the limited efficacy of single-agent therapies, driving the search for novel therapeutic approaches. The water-soluble molybdenum cluster complex Na₅Cs₃[{Mo₆I₈}(S₂O₃)₆]·3H₂O, developed in this study, represents a unique compound that combines a strong chemotherapeutic effect, achieved through the controlled release of sulfur-containing gas-signaling molecules (H₂S and SO₂) during hydrolysis, with a radiodynamic effect, enabled by the ability of the cluster to generate singlet oxygen (¹O₂) under X-rays. The results of <i>in vitro</i> experiments confirmed significant cytostatic effects on cancer cells, while <i>in vivo</i> studies using Nu/J mice xenografted with HeLa tumors showed substantial tumor growth inhibition when the cluster was administered subcutaneously in combination with X-ray irradiation. Overall, the dual functionality of the cluster, along with the slow release and prolonged retention of the complex in tumor tissues, makes it a highly promising candidate for advanced cancer treatment strategies, particularly when integrated with conventional radiotherapy.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional biomaterials and machine learning approaches for phenotyping heterogeneous tumor cells and extracellular vesicles.","authors":"Rutwik Joshi, Raheel Ahmad, Karl Gardner, Hesaneh Ahmadi, Chau-Chyun Chen, Shannon L Stott, Wei Li","doi":"10.1039/d5bm00577a","DOIUrl":"https://doi.org/10.1039/d5bm00577a","url":null,"abstract":"<p><p>Heterogeneity in cancer is known to be a contributor to the formation of metastatic lesions, poor prognosis, and ultimately undermines therapeutic efficacy. This same tumor heterogeneity is reflected in circulating tumor cells (CTCs) and tumor derived extracellular vesicles (EVs), offering a less invasive snapshot into tumor status. The isolation and phenotypic characterization of CTCs and EVs has attracted significant interest in recent years as they offer great potential for deciphering the molecular basis of disease progression and the development of precision therapies. Next-generation biomaterials and advanced machine learning paradigms are transforming how we decipher phenotypic heterogeneity in these circulating biomarkers and help provide new insights into tumor biology and therapy resistance. In this review, we first briefly describe biomaterial-based platforms for the isolation of CTCs and EVs, followed by a detailed discussion of the pivotal role of phenotypic profiling and molecular identification. Finally, we provide a review of emerging biomaterial-based approaches that enable selective sorting, profiling, and detection of CTCs and EVs. In this process, we categorize the most widely utilized biomaterials into polymer-based materials, quantum dots and multifunctional magnetic nanospheres, fluorescent antibodies, Surface Enhanced Raman Spectroscopy (SERS) vectors, DNA-based multi-aptamer probes, cell-imprinted substrates, and silver nanoclusters. We then explore the application of machine learning algorithms in biomarker profiling of CTCs, extending to EVs. Furthermore, we provide a comprehensive analysis of relevant clinical studies and critically examine future challenges and research trajectories in this rapidly evolving field.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}