ACS Sensors最新文献

{"title":"Ionic Liquid-Functionalized CNT Electrochemical Platform Governed by a Dual-Path Sensing Mechanism for Versatile VOC Detection","authors":"Han Li,Yusen Guo,Qiushi Feng,Rui He,Zilun Yin,Sisi Huang,Yan Zhang,Sisi Feng,Xuelei Liang,Qiliang Li","doi":"10.1021/acssensors.6c01205","DOIUrl":"https://doi.org/10.1021/acssensors.6c01205","url":null,"abstract":"Room-temperature ionic liquids (RTILs) have garnered significant attention as advanced electrolytes for electrochemical gas sensors owing to their negligible volatility and wide electrochemical windows. However, their application in detecting volatile organic compounds (VOCs) is constrained by high viscosity (limiting mass transport) and the sluggish electrooxidation kinetics of most VOCs. Supported ionic liquid phase (SILP) materials, which immobilize RTILs on porous supports to amplify the gas–liquid interface, present a promising yet underexplored avenue for addressing these challenges. Herein, we develop a novel electrochemical platform configured with high-purity semiconducting carbon nanotube (CNT) films serving as both working electrodes and scaffolds to immobilize RTILs to construct a nanostructured SILP electrolyte. This architecture simultaneously maximizes the active gas–liquid interface, minimizes the gas diffusion distance, and ensures efficient charge transport. The platform enables quantitative detection of ethanol and isopropanol, exhibiting well-defined linear responses from 0–800 ppm and 0–600 ppm, respectively, with distinctive non-monotonic patterns at higher concentrations. Mechanistic investigations reveal that the sensing process diverges from conventional faradaic electrochemistry and is instead governed by a dual-path mechanism: competitive adsorption between oxygen and VOC molecules at the SILP surface, coupled with VOC absorption into the bulk phase. This unique mechanism, which circumvents the limitations of direct electrooxidation by exploiting distinct interactions between VOCs and the SILP, provides inherent discriminative capability. Consequently, our work establishes the CNT-SILP system as a versatile, miniaturization-ready platform for room-temperature VOC sensing and paves the way for novel intelligent gas sensors.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"14 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2026-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149360320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Field-Deployable and Ultrasensitive Surface-Enhanced Raman Spectroscopy for Waterborne Arsenic Monitoring Based on Headspace Formation of Arsenic–Silver Nanocomposites","authors":"Zhinan Chen,Zhankuo Zhang,Xiangyu Liu,Kailai Xu,Yue Qi,Jinyi Zhang,Chengbin Zheng","doi":"10.1021/acssensors.6c03929","DOIUrl":"https://doi.org/10.1021/acssensors.6c03929","url":null,"abstract":"Millions of people worldwide are exposed to arsenic-contaminated water, driving an urgent need for field-deployable analytical tools. However, direct, label-free surface-enhanced Raman spectroscopy (SERS) detection of arsenic is hindered by its weak Raman activity and complex matrix interference. Herein, we report an ultrasensitive SERS platform that addresses these issues by integrating hydride generation, headspace solid-phase extraction, and in situ substrate formation into a single step. The target arsenic is converted to volatile AsH3, which simultaneously reduces Ag+ to silver nanoparticles (AgNPs) and codeposits with its oxidation products to form arsenic-associated AgNP composites (As-AgNPs). This design eliminates the need for external Raman reporters or prefabricated SERS substrates and, critically, enables direct fingerprint identification of arsenic via characteristic peaks at 425 and 790 cm–1 under the tested conditions. The in situ generated dense AgNP hotspots, synergized with headspace preconcentration, afford a 2.6% signal relative standard deviation at 10 ppb As(III) (n = 21) and a detection limit as low as 0.085 ppb, alongside tolerance to the coexisting ions under the tested conditions. Field testing at the Minjiang River using a portable Raman spectrometer yielded results in agreement with atomic fluorescence spectrometry (t-test, 95% confidence), with a total assay time of 30 min for six parallel samples. This work provides a robust, field-deployable solution for direct trace arsenic monitoring by coupling analyte collection with reaction-synchronized SERS substrate formation.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"9 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2026-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149368256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"“Lab-on-Double Confinement-Pipette Tip”: CDsCu/Mn@MPDA-Driven Portable Intelligent Sensor for In-Situ Rapid Detection of Pesticide Residues","authors":"Mengyu Li,Zhao Liang,Qingshu Ge,Weichao Wu,Tong Ye,Suizheng Sun,Maoling Hu,Weicheng Xu,Hui Li,Ganghui Li,Xin Zhang,Xiaoyu Yu,Xue Yang,Miaomiao Xie,Ziyang Shang,Quanxi Wang,Run Ai,Yuwei Ren,Yingwang Ye","doi":"10.1021/acssensors.6c02886","DOIUrl":"https://doi.org/10.1021/acssensors.6c02886","url":null,"abstract":"The development of high specificity, ultrasensitive, and portable detection technologies is conducive to monitoring pesticide residues in the environment, thereby promoting the scientific use of pesticides. Here, a dual-confined sensitization “lab-on-pipette tip” was developed for colorimetric-photothermal in-situ detection of carbosulfan by encapsulating nanomaterials within the hydrogel matrix on a pipette tip. As the signal transduction core, an interfacial-enhanced mesoporous polydopamine was loaded with dual-metal carbon dots (CDsCu/Mn@MPDA) to mimic high-efficiency peroxidase activity. The synergy between the bimetallic sites lowered the energy barrier for the reduction of H2O2, generating abundant hydroxyl radicals. Crucially, the reducing sulfur species generated from acidified carbosulfan inhibit the activity of CDsCu/Mn@MPDA by triggering radical-scavenging reactions, generating target concentration-dependent signals. Furthermore, CDsCu/Mn@MPDA was encapsulated in the hydrogel and gelled at the pipette tip to construct an integrated platform. The double-confinement effect enhanced reaction-component contact efficiency, resulting in a 40-fold compression of the linear response range for carbosulfan residue detection. The sensing platform exhibited a response range of 0–1200 µM in solution. Strikingly, this range was compressed to 0–30 µM within the spatially confined microcavity. This on-demand tunable range platform slashes per-assay costs by ∼99.7% vs liquid chromatography mass spectrometry, bridging the lab-to-field gap for environmental sensing.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"23 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2026-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149368255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasensitive and Multiplex Detection of RNAs with Single-Nucleotide Resolution Using Lambda Exonuclease-Assisted Probe Signal Amplification-Rolling Circle Amplification","authors":"Yue Zhang,Xing Ke,Kun Yin,Xu Wang","doi":"10.1021/acssensors.6c02576","DOIUrl":"https://doi.org/10.1021/acssensors.6c02576","url":null,"abstract":"Rolling circle amplification (RCA) is a simple and efficient isothermal enzymatic reaction. However, its linear amplification kinetics and unsatisfactory multiplex detection capability severely limit further improvements in detection sensitivity and application flexibility. In this work, we developed a lambda exonuclease-assisted probe signal amplification strategy termed LEPA, integrated with endonuclease-mediated exponential RCA for the sensitive detection of target nucleic acids. This system enables precise target recognition through ligase and padlock probes, which, in turn, initiate cascaded RCA for efficient signal amplification. Through a cyclic “binding-cleavage-dissociation” process between E-probes and amplification products, the system achieves dual signal amplification and single-nucleotide resolution. The LEPA-RCA system demonstrates exceptional detection sensitivity and specificity, allowing precise discrimination of alleles with a mutation frequency as low as 0.1% and supporting multiplex intracellular RNA detection. In clinical validation, this method effectively distinguished the expression levels of serum miR-16-5p and miR-92a-3p between healthy individuals and colorectal cancer (CRC) patients, showing high consistency with RT-qPCR results. Moreover, it achieved excellent diagnostic performance (AUC = 0.85). With a modular design, the LEPA-RCA system serves as a robust platform for high-precision multiplex nucleic acid detection in complex biological matrices, showing great potential for clinical translation.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"34 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2026-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149360319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Endogenously Gated DNA Nanodevice Enables Mitochondria-Specific FEN1 Monitoring in Response to Therapeutic Challenge.","authors":"Lie Li, Baozhu Hu, Chang Liu, Yu Wang, Bin Ning, Xuerui Zhang, Jin Jiao","doi":"10.1021/acssensors.6c03035","DOIUrl":"https://doi.org/10.1021/acssensors.6c03035","url":null,"abstract":"<p><p>Monitoring therapy-induced molecular changes within subcellular compartments is crucial for understanding cellular stress and providing clues to treatment response. Mitochondrial base excision repair (BER) enzymes have emerged as promising indicators of therapeutic response, yet effective tools for their on-site and dynamic monitoring remain scarce. Here, we report an endogenously gated DNA nanodevice (EGDN) capable of real-time, mitochondria-specific monitoring of flap endonuclease 1 (FEN1), a key BER enzyme, under therapeutic challenge. The EGDN remains silent during transport and is sequentially activated by the mitochondria-encoded ribosomal RNA and FEN1, thus achieving spatial control over FEN1 imaging. This conditional regulation pattern reduces nonspecific signals and sharpens the spatial resolution of FEN1 visualization within mitochondria. We further demonstrate that EGDN dynamically tracks mitochondrial FEN1 fluctuations in tumor cells undergoing chemotherapy or radiation therapy, providing a molecular readout of treatment-associated alterations. Collectively, this work establishes a robust biosensing toolbox for dissecting the BER process at the subcellular level, with broad implications for cancer early diagnosis and targeted therapy.</p>","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":" ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2026-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149356941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzyme-Induced Signal Cycling Amplification on a FET-Based POCT Biosensor for Ultrasensitive Tetracycline Detection in Food and Environmental Samples.","authors":"Yang Huang, Tao Liu, Zhili Zhang, Jidong Jiang, Yifei Gao, Wenzhuo Tang, Ming Tian, Yiqing Wang","doi":"10.1021/acssensors.6c03573","DOIUrl":"https://doi.org/10.1021/acssensors.6c03573","url":null,"abstract":"<p><p>Tetracycline (TC), a widely used broad-spectrum antibiotic, poses severe risks to human health through environmental leakage (soil and water) and food chain transmission. However, the low concentration of TC in environmental samples and the complexity of sample matrices pose significant challenges for its rapid, sensitive, and on-site detection. Here, we present a portable point-of-care testing (POCT) device for on-site determination of TC, based on a DNA-functionalized field-effect transistor (FET) biosensor. By implementing an Exonuclease III (Exo III)-assisted cycling amplification strategy on the FET surface, the biosensor efficiently recognizes trace TC and amplifies the signal through consecutive release of reporter probes during nicking reactions. Under optimal conditions, the FET biosensor achieves a wide linear range from 1 pM to 1 μM, a low detection limit of 0.3 pM and completes ultrasensitive detection within 70 min. A rapid microfluidic channel is also designed to transport water samples efficiently and provide reaction zones for recognition and signal amplification. When connected to a printed circuit board and a mobile application via Bluetooth, the proposed system can rapidly report TC levels on-site, demonstrating good accuracy and reliability in river water, milk, and honey samples. This work paves the way for integrated, ultrasensitive detection of antibiotic pollutants, offering a powerful and portable POCT device for early warning and source tracing of pollutant leakage.</p>","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":" ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2026-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149339892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanomaterial-Enabled Electrochemical Biosensing Platforms for Circulating Tumor Cell Analysis.","authors":"Xinggang Yang, Guonian Zhu, Zhe Wen, Hongxia Su, Xuping Sun, Weimin Li, Zhoufeng Wang","doi":"10.1021/acssensors.6c02238","DOIUrl":"https://doi.org/10.1021/acssensors.6c02238","url":null,"abstract":"<p><p>Circulating tumor cells (CTCs) are valuable liquid biopsy markers for assessing tumor heterogeneity, monitoring minimal residual disease (MRD), and guiding personalized therapy. However, their rarity in blood and phenotypic changes during epithelial-mesenchymal transition (EMT) limit reliable detection. Electrochemical interfacial biosensors offer a promising solution due to their high sensitivity, miniaturization potential, and compatibility with microfluidics. This review summarizes recent advances in nanomaterial-enabled electrochemical CTC biosensing, focusing on interface engineering, signal transduction, and clinical translation. We highlight strategies such as multivalent recognition, engineered nanotopography, antifouling interfaces, and stimulus-responsive release that enhance CTC capture in whole blood. We also discuss nanozyme-based amplification, ratiometric calibration, and multiplexed electrochemical detection for phenotypic profiling beyond simple enumeration. Finally, we outline key translational requirements, including standardized benchmarking, clinical cohort validation, and artificial intelligence (AI)-assisted data analysis. Integrating nanostructured interfaces with multiplexed detection and standardized whole-blood validation may accelerate electrochemical CTC analysis toward routine, phenotype-aware liquid biopsy.</p>","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":" ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2026-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149339893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Alkyne-Probe for Raman Imaging of Cellular Monoacylglycerol Lipase.","authors":"Samir F El-Mashtoly, Annaleah Hanske, Constanze Schultz, Axel Hentsch, Jamile Mohammad Jafari, Jörg Benz, Bernd Kuhn, Yelena Mostinski, Andreas Topp, Dominik Heer, Lars W van Abswoude, Tobias Meyer-Zedler, Thomas Bocklitz, Senthilvelrajan Kaniyappan, Rainer E Martin, Mario van der Stelt, Christoph Krafft, Uwe Grether, Marc Nazare, Anne T G De Paepe, Jürgen Popp","doi":"10.1021/acssensors.6c02475","DOIUrl":"https://doi.org/10.1021/acssensors.6c02475","url":null,"abstract":"<p><p>Monoacylglycerol lipase (MAGL) plays a central role in endocannabinoid signaling and represents a key therapeutic target for neurodegenerative and inflammatory diseases, cancer, and psychiatric conditions. Sensitive tools for monitoring MAGL distribution in cells are therefore essential for elucidating its biological function. Here, we report the design, synthesis, and cellular application of the covalent, alkyne-tagged MAGL probe 1, which enables enzyme-associated vibrational imaging through detection of the enzyme-bound probe by spontaneous Raman and stimulated Raman scattering (SRS) microscopy. The probe exhibits a distinct C≡C stretching band at 2210 cm-1 within the \"cell-silent\" region, enabling intracellular detection of the non-chromophoric enzyme across concentrations ranging from 0.5-25 μM, including robust visualization at 0.5 μM, a concentration relevant to clinically advanced MAGL inhibitors. Spontaneous Raman and SRS imaging revealed enrichment of the enzyme-bound probe at lipid-rich intracellular interfaces, consistent with lipid droplet-associated MAGL localization, while SRS enabled rapid, high-contrast subcellular mapping. Control experiments, including competitive inhibition, confirmed that the observed signal arises predominantly from the covalently bound probe 1 rather than unreacted species. Target specificity was further supported by activity-based protein profiling, excluding nonspecific lipid accumulation. Rather than directly imaging the enzyme itself, the vibrational signal reports on covalent probe-enzyme engagement as a surrogate for enzyme localization. Correlative fluorescence lifetime imaging microscopy using a matched BODIPY-labeled MAGL fluoroprobe confirmed target engagement and provided orthogonal vibrational and fluorescence readouts. These findings demonstrate the feasibility of covalent enzyme-targeted Raman imaging as a complementary modality to fluorescence-based approaches for imaging non-chromophoric enzymes in cells using matched molecular pair probes.</p>","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":" ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2026-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149339890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Electrochemiluminescence Biosensing Platform Based on a Hydrogel-AAO Nanochannel Membrane for GFAP Detection.","authors":"Junjie Lai, Jiaming Chen, Hongyang Huang, Jie Liu, Zhiyan Zou, Jilai Liu, Zhenyu Lin, Guolin Hong","doi":"10.1021/acssensors.6c03340","DOIUrl":"https://doi.org/10.1021/acssensors.6c03340","url":null,"abstract":"<p><p>Electrochemiluminescence (ECL) biosensors are widely used for ultrasensitive bioanalysis; however, their practical application is often limited by insufficient interface stability under complex detection conditions. Herein, an integrated hydrogel-AAO membrane (HAM) as a novel composite ion-exchange membrane is developed for constructing stable ECL biosensing interfaces. In the HAM architecture, the agarose hydrogel provides a three-dimensional, biocompatible matrix for antibody immobilization and target recognition, while the highly ordered AAO nanochannels serve as an efficient ion-transport and signal transduction pathway. The composite membrane enables effective coupling between interfacial biorecognition events and ECL signal modulation while physically separating the recognition layer from the ECL reaction region. Using glial fibrillary acidic protein (GFAP), a clinically relevant biomarker for Alzheimer's disease, as the model analyte, the proposed sensor achieved a detection limit of 191.6 fg/mL with a linear range from 1 pg/mL to 100 ng/mL. The sensor further demonstrated reliable quantification of GFAP in human serum samples, showing good agreement with a commercial ELISA kit. This work establishes the HAM as a versatile composite ion-exchange membrane for ECL biosensing and provides a general design framework for developing stable and sensitive ECL biosensors for clinical analysis.</p>","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":" ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2026-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149339891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quo Vadis (Where to), Illicit Drug Testing? Status, Developments, and Perspectives.","authors":"Yordan Kostov, Leonardo M Angelone, Elena Koustova","doi":"10.1021/acssensors.6c03922","DOIUrl":"https://doi.org/10.1021/acssensors.6c03922","url":null,"abstract":"<p><p>Drug testing is a critical tool used in many settings that span clinical care, employers, forensics, and law enforcement. It strives to accurately identify and quantify drugs with high addiction potential by utilizing the available analytical methods and sensors. This editorial offers an insight into the evolution of drug testing into its current set of methods and looks at the current trends that aim to transform the current practice, with a particular focus on the role of sensors and sensor-based devices. Unmet needs for different use cases are discussed, particularly the need for sensors capable of faster, lower-cost, and more specific detection at the point of need. An overview of the efforts in the state-of-the-art technologies that are developed while aiming to address these needs is presented. The paper aims to encourage further work on devices and sensors in the area and highlights the interests of NIDA.</p>","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":" ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149316094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}