ACS Sensors最新文献_第3页

A High-Performance Surface Acoustic Wave Humidity Sensor with Uniform Multiwrinkled Graphene Oxide Film

IF 8.9 1区化学

ACS Sensors Pub Date : 2024-12-12 DOI: 10.1021/acssensors.4c02177

Gai Yang, Li Peng, Shiqian Cai, Huikai Xie, Feng Gao, Xiaoyi Wang

{"title":"A High-Performance Surface Acoustic Wave Humidity Sensor with Uniform Multiwrinkled Graphene Oxide Film","authors":"Gai Yang, Li Peng, Shiqian Cai, Huikai Xie, Feng Gao, Xiaoyi Wang","doi":"10.1021/acssensors.4c02177","DOIUrl":"https://doi.org/10.1021/acssensors.4c02177","url":null,"abstract":"Humidity sensors were widely used in Internet of Things (IoT) applications. According to medical respiration monitoring, noncontact sensing, and human–computer interface requirements, fast response and high repeatability are important for efficient and precise signal acquisition. Previous research works mainly focus on sensitivity improvement with the scarification of response time, stability, and repeatability. In this paper, we proposed the surface acoustic wave (SAW) humidity sensors with comprehensive performance using the uniform multiwrinkled graphene oxide (GO) films as the sensing material obtained by vacuum filtration and liquid phase transfer method. The multiwrinkled GO films offered controllable thickness (thin to 29 nm), uniform wafer-level fabrication (2 in.), and abundant wrinkle, making them become effective sensitive films for SAW humidity sensors due to numerous adsorption sites and transfer channels for water molecules. The experimental results showed that the sensors can obtain high sensitivity (10.5 kHz/RH%@60 nm thick film), ultrafast response (∼45 ms), good stability (variation amplitude ∼0.1%), repeatability (variation amplitude ∼1%), and wafer-level fabrication capability demonstrating their practical applications for medical respiration monitoring, noncontact sensing, and human–computer interface.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"9 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142809404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA Reaction Network Central Controller for Dynamic Spatiotemporal Logical Assembly and Its Application for Rational Design of Fluorometric/Electrical Biosensing

IF 8.9 1区化学

ACS Sensors Pub Date : 2024-12-11 DOI: 10.1021/acssensors.4c02493

Xin Ma, Jian Wang, Sai Zhang, Xiaohai Yan, Kewei Feng, Yane Luo, Junfeng Yao, Tianliang Liu, Yahong Yuan, Tianli Yue, Qinglin Sheng

{"title":"DNA Reaction Network Central Controller for Dynamic Spatiotemporal Logical Assembly and Its Application for Rational Design of Fluorometric/Electrical Biosensing","authors":"Xin Ma, Jian Wang, Sai Zhang, Xiaohai Yan, Kewei Feng, Yane Luo, Junfeng Yao, Tianliang Liu, Yahong Yuan, Tianli Yue, Qinglin Sheng","doi":"10.1021/acssensors.4c02493","DOIUrl":"https://doi.org/10.1021/acssensors.4c02493","url":null,"abstract":"This work introduces a fluorometric/electrical dual-biosensing logic system based on a DNA reaction network (DRN). This system was used to spatiotemporally modulate the kinetic behavior of DNA nanostructures. The system, acting as a programmable and modulative central controller introduced to implement, enabled the monitoring of the target gliotoxin. The DRN encompasses multiple pathways and provides a potential mechanistic way to develop dynamic networks that can evolve under directional controllable conditions. We demonstrated the implementation of a DRN to control the assembly and disassembly of a DNA conveyor belt. By exposing the responsive switches of the DNA conveyor belt, the DRN activates the operation of fluorescent DNA-driving axes based on the aggregation-induced emission effect, enabling signal generation and collection through continuous rolling on the surface of the DNA conveyor belt. The biosensor was employed to monitor gliotoxin, and under optimal conditions, dual-signal detection was achieved at 1.14 × 10–7 and 2.45 × 10–7 μg·mL–1. The biosensor was integrated with a handheld electrochemical workstation, which enabled the successful monitoring of gliotoxin. This strategy enables self-tuning control and the multilayer hierarchical assembly of kinetic behaviors and is applicable to diverse fields such as biometric systems, medical diagnosis, and logic computing.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"12 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142809405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fe Single-Atom and Fe Cluster-Coupled N, S Co-doped Carbon Nanomaterial-Based Flexible Electrochemical Sweat Biosensor for the Real-Time Analysis of Uric Acid and Tyrosine

IF 8.9 1区化学

ACS Sensors Pub Date : 2024-12-11 DOI: 10.1021/acssensors.4c01844

Fan Shi, Zejun Zhang, Behisht Ara, Zhitan Huang, Qifeng Gui, Ahmad Mansoor, Wei Sun

{"title":"Fe Single-Atom and Fe Cluster-Coupled N, S Co-doped Carbon Nanomaterial-Based Flexible Electrochemical Sweat Biosensor for the Real-Time Analysis of Uric Acid and Tyrosine","authors":"Fan Shi, Zejun Zhang, Behisht Ara, Zhitan Huang, Qifeng Gui, Ahmad Mansoor, Wei Sun","doi":"10.1021/acssensors.4c01844","DOIUrl":"https://doi.org/10.1021/acssensors.4c01844","url":null,"abstract":"Fe single-atom and Fe cluster-coupled N, S co-doped carbon nanomaterials (FeSA–FeONC-NSC) were prepared through a two-step high-temperature pyrolysis process using Gelidium corneum enriched with C, Fe, O, N, and S as precursors. The analysis by aberration-corrected scanning transmission electron microscopy and X-ray absorption spectroscopy revealed the presence of single-atom Fe in Fe–N4 coordination structures, along with small clusters as Fe–O-coordinated Fe2O3. Single-atom Fe in the form of Fe2+/Fe3+ provides more electrocatalytic active sites, which synergistically accelerates the charge migration process in the assembly of FeSA–FeONC-NSC with Fe2O3 clusters. The flexible nonenzymatic sensor, based on FeSA–FeONC-NSC and fabricated using a polydimethylsiloxane substrate, exhibited excellent catalytic activity for both uric acid (UA) and tyrosine (Tyr). Low detection limits for UA (0.14 μmol L–1) and Tyr (0.03 μmol L–1) were observed by using chronoamperometry in artificial sweat. The in situ detection of sweat was performed in combination with an integrated circuit board affixed to human skin, and the results were generally consistent with those of the high-performance liquid chromatography method. Therefore, FeSA–FeONC-NSC serves as a good modifier for wearable electrochemical sweat sensor applications.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"46 12 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142809402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanochromic Chameleon Packaging Based on Polydiacetylene

IF 8.9 1区化学

ACS Sensors Pub Date : 2024-12-10 DOI: 10.1021/acssensors.4c02694

Bratati Das, Yu Uchikura, Naoji Matsuhisa, Yuya Oaki, Miles Pennington, Kaori Sugihara

{"title":"Mechanochromic Chameleon Packaging Based on Polydiacetylene","authors":"Bratati Das, Yu Uchikura, Naoji Matsuhisa, Yuya Oaki, Miles Pennington, Kaori Sugihara","doi":"10.1021/acssensors.4c02694","DOIUrl":"https://doi.org/10.1021/acssensors.4c02694","url":null,"abstract":"We demonstrated a mechanochromic chameleon packaging that quantitatively visualizes damage based on the polydiacetylene blue-to-red color transition. The applied pressures on the damage can be conveniently read by taking a photograph via a homemade mobile app called the “Pressure Analysis App”. The key aspects of the development were 1) the adjustment of the sensitivity by adding guest molecules into the PDA matrix, 2) quantitative calibration, and 3) the accurate reading of the pressure from RGB images based on the calibration. The force sensitivity of polydiacetylene on cellulose was characterized in the range of 30–100 nN (2.59–8.86 MPa) at the nanoscale by dual friction force/fluorescence microscopy, and in the range of 0.7–2.2 N (0.11–0.35 MPa) at the macroscopic scale by a friction tester. The scanning speed was found to be one of the key parameters, where the force sensitivity above 50 mm/s was 6.45 times larger than that below 10 mm/s. The developed mechanochromic packaging system can be used for transporting electronics and medical tools, such as vaccine vials, food, etc., more safely and reliably in the future.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"13 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142797431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Refractive Index Morphology Imaging Microscope System Utilizing Polarization Multiplexing for Label-Free Single Living Cells 利用偏振复用技术的折射率形态学成像显微系统，用于无标签单个活细胞

IF 8.9 1区化学

ACS Sensors Pub Date : 2024-12-09 DOI: 10.1021/acssensors.4c02484

Huijun Wang, Lu Zhang, Chen Fan, Jie Huang, Weihao Zhao, Zewen Yang, Lifang Tian, Hong Zhao, Cuiping Yao

{"title":"Refractive Index Morphology Imaging Microscope System Utilizing Polarization Multiplexing for Label-Free Single Living Cells","authors":"Huijun Wang, Lu Zhang, Chen Fan, Jie Huang, Weihao Zhao, Zewen Yang, Lifang Tian, Hong Zhao, Cuiping Yao","doi":"10.1021/acssensors.4c02484","DOIUrl":"https://doi.org/10.1021/acssensors.4c02484","url":null,"abstract":"Detections of internal substances and morphologies for label-free living cells are crucial for revealing malignant diseases. With the phase serving as a coupling of refractive index (RI) (marker for substances) and thickness (morphology), existing decoupling methods mainly rely on complex integrated systems or extensive optical field information. Developing simple and rapid decoupling methods remains a challenge. This study introduces a refractive index morphology imaging microscope (RIMIM) system utilizing polarization multiplexing for label-free single living cells. By simultaneous degree of circular polarization (DOCP) imaging and noninterferometric quantitative phase imaging (QPI), the intracellular refractive index distribution (IRID) and morphology can be decoupled. The optical thickness calculated from the phase is input into the circular depolarization decay model (CDDM) of degree of circular polarization to retrieve IRID. Subsequently, the thickness can be decoupled from phase result using retrieved IRID. Experiments conducted on mouse forestomach carcinoma (MFC) cells and human kidney-2 cells (HK-2) demonstrated the RIMIM system’s ability to retrieve IRID and decouple fine morphology. Additionally, the RIMIM system effectively detected membrane damage and changes in erastin-induced ferroptotic HK-2 cells, with average and root-mean-square of surface folds 65.5% and 70.0% higher than those of normal HK-2 cells. Overall, the RIMIM system provides a simple and rapid method for decoupling RI and fine morphology, showing great potential for label-free live cells’ cytopathology detection.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"3 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142797434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface Plasmon-Driven Versatile Enhancement of Chemosensing

IF 8.9 1区化学

ACS Sensors Pub Date : 2024-12-09 DOI: 10.1021/acssensors.4c01985

Chunhui Li, Dinghai Xu, Huan Liu, Wenxing Xu, Bo Wang, Qingguo He, Wei Xu, Yanyan Fu, Huizi Li, Jiangong Cheng

{"title":"Surface Plasmon-Driven Versatile Enhancement of Chemosensing","authors":"Chunhui Li, Dinghai Xu, Huan Liu, Wenxing Xu, Bo Wang, Qingguo He, Wei Xu, Yanyan Fu, Huizi Li, Jiangong Cheng","doi":"10.1021/acssensors.4c01985","DOIUrl":"https://doi.org/10.1021/acssensors.4c01985","url":null,"abstract":"Chemo-sensors have deeply integrated into various facets of our daily lives. To further satisfy the increasing performance demand, the current attempts are mainly centered on materials science approaches, usually involving time-& labor-consuming structure designing, synthesis, and modification. To date, it remains largely unexplored to enhance sensing material performance at the fundamental physical level by strategic exploitation of optical properties. In this work, we proposed a facile and versatile approach for improving the material performance by strategically utilizing the surface plasmon resonance─a characteristic property of optical devices. This approach is revealed to have a dual effect on fluorescence-based chemosensing: it amplifies the collection of fluorescence signals and simultaneously expedites the kinetics of chemical reactions. In this work, we developed a surface plasmon-driven fluorescence-based chemosensor that utilizes the 2,4,6-trisformyl phenol-diethylamine (TFP-I) fluorescent probe for the detection of hydrogen peroxide (H2O2) gas molecules. By harnessing the dual-effect induced by surface plasmons, we achieved outstanding sensing performance for H2O2 gas molecules, characterized by 0.0225 ppt sensitivity and an exceedingly low limit of detection. This study substantiates the applicability of the surface plasmon resonance-based optical effect in the realm of fluorescent chemical materials for sensing performance amplification. Beyond this, it pioneers the strategic harnessing of optical effects to manipulate the performance of chemical materials, particularly for the advancement of sensing capabilities.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"21 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142797433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tuning the Localized Microenvironment near a Continuous Glucose Meter to Ensure Monitoring Accuracy and Longevity by Plasma-Induced Grafting Zwitterionic Brushes

IF 8.9 1区化学

ACS Sensors Pub Date : 2024-12-05 DOI: 10.1021/acssensors.4c01921

Syuan-Jia Shin, Pei-Chen Lo, Yen-Ting Wu, Huai-Hsaun Shao, Dai-Jin Li, Yung-Cheng Weng, You-Yin Chen, Ta-Chung Liu

{"title":"Tuning the Localized Microenvironment near a Continuous Glucose Meter to Ensure Monitoring Accuracy and Longevity by Plasma-Induced Grafting Zwitterionic Brushes","authors":"Syuan-Jia Shin, Pei-Chen Lo, Yen-Ting Wu, Huai-Hsaun Shao, Dai-Jin Li, Yung-Cheng Weng, You-Yin Chen, Ta-Chung Liu","doi":"10.1021/acssensors.4c01921","DOIUrl":"https://doi.org/10.1021/acssensors.4c01921","url":null,"abstract":"Diabetes mellitus is a metabolic disorder that affects millions of individuals worldwide. Continuous glucose monitoring (CGM) offers a prevalent method for continuously monitoring interstitial glucose levels instead of traditional self-monitoring of blood glucose (BG), eliminating the need for finger pricking and providing only discrete data. However, challenges in accuracy persist in CGM, including substantial noise interference and tissue fluid erosion, as well as the pH fluctuations in the localized ISF microenvironment during acute inflammation periods. Herein, we reported a facile atmospheric plasma-induced grafting technique to surface functionalize a zwitterionic brush coating on the sensor, with the aim to adjust the sensor's microenvironmental chemistry. The zwitterionic brush-coated CGM (Z-coated CGM) could regulate pH values with a good glucose response in the pH range from 6.2 to 7.6 and a prolonged sensor life over the uncoated sensor. We evaluated the rat practice that the Z-coated CGM consistently outperformed the uncoated in tracking BG fluctuations, with higher correlation coefficients and significant noise reduction for both non-recalibration and recalibration. This technology holds substantial implications for subcutaneous embedded glucose monitors and facilitates CGMs in achieving independence from routine BG fingerstick calibrations.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"7 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrafast Intelligent Sensor for Integrated Biological Fluorescence Imaging and Recognition

IF 8.9 1区化学

ACS Sensors Pub Date : 2024-12-05 DOI: 10.1021/acssensors.4c01839

Yuqing Jian, Wei Gao, Yue Qin, Hao Guo, Xiaoyu Wu, Zhenyan Jia, Huanfei Wen, Zhonghao Li, Zongmin Ma, Xin Li, Jun Tang, Jun Liu

{"title":"Ultrafast Intelligent Sensor for Integrated Biological Fluorescence Imaging and Recognition","authors":"Yuqing Jian, Wei Gao, Yue Qin, Hao Guo, Xiaoyu Wu, Zhenyan Jia, Huanfei Wen, Zhonghao Li, Zongmin Ma, Xin Li, Jun Tang, Jun Liu","doi":"10.1021/acssensors.4c01839","DOIUrl":"https://doi.org/10.1021/acssensors.4c01839","url":null,"abstract":"Fluorescence imaging and recognition are core technologies in targeted medicine, pathological surgery, and biomedicine. However, current imaging and recognition systems are separate, requiring repeated data transfers for imaging and recognition that lead to delays and inefficiency, hindering the capture of rapidly changing physiological processes and biological phenomena. To address these problems, we propose an integrated intelligent sensor for biological fluorescence imaging and ultrafast recognition. This sensor integrates an imaging system based on a photodetector array and a recognition system based on neural networks on a single chip, featuring a highly compact structure, a continuously adjustable optical response, and reconfigurable electrical performance. The unified architecture of the imaging and recognition systems enables ultrafast recognition (19.63 μs) of tumor margins. Additionally, the special organic materials and bulk heterojunction structure endow the photodetector array with strong wavelength dependence, achieving high specific detectivity (3.06 × 1012 Jones) in the narrowband near-infrared range commonly used in biomedical imaging (600–800 nm). After training, the sensor can accurately recognize biological fluorescence edges in real time, even under interference from other colored light noise. Benefiting from its rapidity and high accuracy, we demonstrated a simulated surgical experiment showcasing tumor edge fluorescence imaging, recognition, and cutting. This integrated approach holds the potential to establish a novel paradigm for designing and manufacturing intelligent medical sensors.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"81 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasensitive Electrochemical Vertical Flow Immunoassay for Rapid and Simultaneous Detection of Interleukin-6 and Procalcitonin

IF 8.9 1区化学

ACS Sensors Pub Date : 2024-12-05 DOI: 10.1021/acssensors.4c02257

Xudong Ying, Wenxuan Fu, Min Qi, Lin Zhou, Wei Li, Bin Su

{"title":"Ultrasensitive Electrochemical Vertical Flow Immunoassay for Rapid and Simultaneous Detection of Interleukin-6 and Procalcitonin","authors":"Xudong Ying, Wenxuan Fu, Min Qi, Lin Zhou, Wei Li, Bin Su","doi":"10.1021/acssensors.4c02257","DOIUrl":"https://doi.org/10.1021/acssensors.4c02257","url":null,"abstract":"Rapid and multiplexed detection of biomarkers plays an indispensable role in disease diagnosis. Although paper-based lateral flow immunoassays have been widely used in this field, the speediness and throughput are still challenging issues. Herein, an electrochemical vertical flow immunoassay device (eVFID) is fabricated for rapid, ultrasensitive, and multiplexed detection of inflammatory biomarkers. Working electrodes with excellent electrochemical performance and permeability properties were directly fabricated on the nitrocellulose membrane to enable both the vertical flow of the sample solution and electrochemical detection. This vertical configuration can remarkably improve the speediness of the immunoassay and effectively inhibit the cross-talk reactions among immunomolecules, thus allowing rapid and simultaneous detection of multiplexed biomarkers. Furthermore, a signal amplification strategy based on horseradish peroxidase and tetramethylbenzidine was integrated into the eVFID to substantially increase the sensitivity of the electrochemical detection. A low limit of detection of 0.1 and 0.22 pg mL–1 was obtained for two low-abundance inflammatory biomarkers, interleukin-6 (IL-6) and procalcitonin (PCT), respectively. Finally, using a two-channel eVFID, simultaneous detection of IL-6 and PCT in human plasma samples was successfully realized within 5 min. We believe that the eVFID holds great promise for speedy and high-throughput biomarker detection at the point of care.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"4 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tandem Imaging of Breath Ethanol and Acetaldehyde Based on Multiwavelength Enzymatic Biofluorometry

IF 8.9 1区化学

ACS Sensors Pub Date : 2024-12-05 DOI: 10.1021/acssensors.4c02451

Kenta Iitani, Rintaro Miura, Jihu Lim, Ryotaro Ishida, Kenta Ichikawa, Koji Toma, Takahiro Arakawa, Kohji Mitsubayashi

{"title":"Tandem Imaging of Breath Ethanol and Acetaldehyde Based on Multiwavelength Enzymatic Biofluorometry","authors":"Kenta Iitani, Rintaro Miura, Jihu Lim, Ryotaro Ishida, Kenta Ichikawa, Koji Toma, Takahiro Arakawa, Kohji Mitsubayashi","doi":"10.1021/acssensors.4c02451","DOIUrl":"https://doi.org/10.1021/acssensors.4c02451","url":null,"abstract":"Highly sensitive and selective imaging of human-borne volatile organic compounds (VOCs) enables an intuitive understanding of their concentrations and release sites. While multi-VOC imaging methods have the potential to facilitate step-by-step metabolic tracking and improve disease screening accuracy, no such system currently exists. In this study, we achieved simultaneous imaging of ethanol (EtOH) and acetaldehyde (AcH), the starting molecule and an intermediate metabolite of alcohol metabolism, using a multiwavelength VOC imaging system. The system employed alcohol dehydrogenase-catalyzed substrate oxidation (ADHOX) and reduction (ADHRD) reactions. The oxidation of EtOH by ADHOX in the presence of NAD+ produced NADH, which was subsequently oxidized by diaphorase (DP) with resazurin, leading to the resorufin formation, characterized by red fluorescence (excitation at 560 nm and fluorescence at 590 nm). Reduction of AcH by ADHRD consumed NADH, leading to a decrease in blue fluorescence (ex. 340 nm, fl. 490 nm). Meshes incorporating ADHOX-DP or ADHRD were arranged in tandem in front of a camera. Fluorescence images were captured, while a mixture of gaseous EtOH and AcH was applied by switching between two bandpass filters at 1 Hz. Each mesh exhibited selective responses to the target VOCs, with no significant impact on the dynamic range observed in either the single or tandem configurations (EtOH 1–300 ppm, AcH 0.2–5 ppm). The 90% response time was close after time–domain image differential analysis (EtOH = 26 s and AcH = 15 s). Furthermore, the system enabled simultaneous and quantitative imaging of EtOH and AcH concentrations in the breath after alcohol consumption. It also distinguished differences in alcohol metabolism based on the alcohol dehydrogenase 2 (ALDH2) activity, as indicated by the EtOH/AcH ratio (ALDH2 active vs nonactive: 120.9/0.71 ppm vs 129.2/1.99 ppm).","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"4 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142776831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0