ACS Sensors最新文献_第3页

Integrated Single-Step Duplex CRISPR-Based System for Point-of-Care Detection of Human Immunodeficiency Virus and Treponema pallidum Directly from Whole Blood. 直接从全血中检测人类免疫缺陷病毒和梅毒螺旋体的集成单步双工crispr系统。

IF 10.9 1区化学

ACS Sensors Pub Date : 2026-11-04 DOI: 10.1021/acssensors.6c01767

Ziwen Xu, Yunxiang Wang, Muyun Wei, Ping Miao, Haomin Zhang, Min Li, Shengqi Wang, Zhen Rong, Kunlun He

{"title":"Integrated Single-Step Duplex CRISPR-Based System for Point-of-Care Detection of Human Immunodeficiency Virus and Treponema pallidum Directly from Whole Blood.","authors":"Ziwen Xu, Yunxiang Wang, Muyun Wei, Ping Miao, Haomin Zhang, Min Li, Shengqi Wang, Zhen Rong, Kunlun He","doi":"10.1021/acssensors.6c01767","DOIUrl":"https://doi.org/10.1021/acssensors.6c01767","url":null,"abstract":"Acquired immunodeficiency syndrome (AIDS) and syphilis are two major sexually transmitted diseases that pose serious threats to public health, underscoring the urgent need for fast and sensitive diagnosis for human immunodeficiency virus (HIV) and Treponema pallidum (TP). Here, we developed a single-step duplex CRISPR assay that integrated reverse transcription, recombinase polymerase amplification, in vitro transcription, and CRISPR/Cas12a and Cas13a detection for simultaneous HIV and TP nucleic acid testing. We further engineered a fully integrated, low-cost microfluidic chip that consolidated whole-blood sample processing, nucleic acid release, and single-step duplex CRISPR detection. The chip featured a rapid plasma separator with a press-actuated design that removed inhibitors and extracted plasma within minutes without external equipment. The entire process was coupled with a homemade smartphone-based portable device that provided thermal incubation, dual-channel fluorescence excitation, and automated image analysis via a dedicated mobile application. This integrated system enables \"sample-to-answer\" detection within 30 min directly from whole blood, achieving a limit of detection of 2.5 copies/μL of both HIV and TP and demonstrating 100% sensitivity and specificity concordant with the real-time PCR in 51 cases of clinical samples. This work demonstrates a transformative point-of-care diagnostic platform that combines minimal user steps, equipment independence, and robust multiplex detection for decentralized pathogen testing.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":" ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149331695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical Trace Ammonia Sensors with Ultra-Fast Response Time and Stability. 具有超快速响应时间和稳定性的光学痕量氨传感器。

IF 10.9 1区化学

ACS Sensors Pub Date : 2026-11-04 DOI: 10.1021/acssensors.6c03788

Andreas Matijevic, Matthias Schwar, Sergey M Borisov, Torsten Mayr

{"title":"Optical Trace Ammonia Sensors with Ultra-Fast Response Time and Stability.","authors":"Andreas Matijevic, Matthias Schwar, Sergey M Borisov, Torsten Mayr","doi":"10.1021/acssensors.6c03788","DOIUrl":"https://doi.org/10.1021/acssensors.6c03788","url":null,"abstract":"Free ammonia poses a threat to aquatic systems already in very low concentrations (<25 µg·L-1) but is currently challenging to continuously monitor at these levels using effortless and compact analytical tools. Herein, we present an optical chemical sensor which overcomes the stability limitations of ammonia sensors based on pH-sensitive dyes. The new aza-BODIPY dye incorporates a N,N-diisopropylaminophenyl group that induces pH sensitivity via the photoinduced electron transfer effect in the neutral form (pKa of 4.25) without significant alteration of the absorption spectrum. The new indicator dye efficiently absorbs in the red part of the electromagnetic spectrum (λabs = 654 nm, ε = 92,600 L·mol-1·cm-1) and shows moderately strong fluorescence for the cationic form (λem = 689 nm, Φ = 0.24) and no emission from the neutral form. The ammonia sensors are designed by immobilization of the indicator into a hydrophilic matrix (polyurethane hydrogel D4) with porous glass particles along with a protective hydrophobic silicone layer and PTFE membrane on top that eliminates cross-talk to pH. The sensors exhibit a low limit of detection (0.26 µg·L-1) and a limit of quantification (1.23 µg·L-1), along with fast response and recovery times of 18 s and 6-7 min, respectively. The sensor shows excellent reversibility and long-term stability over several weeks. Field tests demonstrate the suitability of new ammonia sensors for fish farming and wastewater treatment. Ammonia concentrations determined are in good agreement with those obtained by a photometric reference method.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":" ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149316093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Force-Transmission-Enhanced Dual-Modal Piezoelectric Sensing for Monitoring and Analysis of Cardiovascular Health State. 力传输增强双模态压电传感用于心血管健康状态监测与分析。

IF 10.9 1区化学

ACS Sensors Pub Date : 2026-11-04 DOI: 10.1021/acssensors.6c03597

Guangyan Liu, Changning Tian, Yutian Mi, Qingcheng Liu, Zhiming Song, Qirui Zhao, Yuanzheng Zhang, Daxiang Cui, Haiwu Zheng

{"title":"Force-Transmission-Enhanced Dual-Modal Piezoelectric Sensing for Monitoring and Analysis of Cardiovascular Health State.","authors":"Guangyan Liu, Changning Tian, Yutian Mi, Qingcheng Liu, Zhiming Song, Qirui Zhao, Yuanzheng Zhang, Daxiang Cui, Haiwu Zheng","doi":"10.1021/acssensors.6c03597","DOIUrl":"https://doi.org/10.1021/acssensors.6c03597","url":null,"abstract":"Monitoring and analyzing cardiovascular health status can reduce the incidence and mortality of cardiovascular diseases, yet this is difficult to implement with current technologies due to the limited information dimensions of single-node sensors and poor skin-sensor biomechanical coupling. To address these issues, this work innovatively constructs a flexible piezoelectric sensing pair (FPSP) mainly composed of BTO/P(VDF-TrFE) piezoelectric nanofiber and a force-transfer-enhanced structure, which can simultaneously capture pulsation signals from the brachial artery and heartbeat. Personalized force-transmission layers with different Young's moduli were designed specifically for the pulsation signal characteristics of the brachial artery and heartbeat to increase the sensing area for pulse signals and reduce transmission loss of heartbeat signals, respectively. The acquired dual-modal data contain richer physiological information compared to single-node data, enabling accurate calculation of pulse transit time. In addition, across various neural network architectures, models trained using dual-modal data achieved significantly higher classification accuracy for common cardiovascular diseases than those trained using single-node data, and they also performed excellently in cardiovascular health prediction tasks. This work not only proposes a new strategy for optimizing wearable sensor performance by enhancing biomechanical coupling but also provides a promising technical framework for the precise analysis and continuous monitoring of cardiovascular health.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":" ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149331693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Förster Resonance Energy Transfer Imaging of Cell-Surface GlycoRNAs: Elucidating GlycoRNA-Cell Migration Correlation and Profiling of Associated RNA-Binding Proteins. Förster细胞表面GlycoRNAs的共振能量转移成像：阐明glycorna -细胞迁移相关性和相关rna结合蛋白的谱图。

IF 10.9 1区化学

ACS Sensors Pub Date : 2026-11-04 DOI: 10.1021/acssensors.6c00869

Meilun Tan, Tingju Ren, Yue Wang, Chunguang Yang, Zhangrun Xu

{"title":"Förster Resonance Energy Transfer Imaging of Cell-Surface GlycoRNAs: Elucidating GlycoRNA-Cell Migration Correlation and Profiling of Associated RNA-Binding Proteins.","authors":"Meilun Tan, Tingju Ren, Yue Wang, Chunguang Yang, Zhangrun Xu","doi":"10.1021/acssensors.6c00869","DOIUrl":"https://doi.org/10.1021/acssensors.6c00869","url":null,"abstract":"Cell‑surface glycosylated RNAs (glycoRNAs) are N‑glycosylated noncoding RNAs and associate with cell‑surface RNA‑binding proteins (csRBPs). Conventional detection methods suffer from destructive processing and diffraction-limited resolution, failing to resolve native distributions. To address this, we developed GlycoFRET‑PLIA (Proximity Labelling & Imaging Analysis), which integrates metabolic azido‑sialic acid labeling, strain‑promoted azide‑alkyne cycloaddition, N3‑kethoxal RNA tagging, and Förster resonance energy transfer (FRET) imaging. GlycoFRET‑PLIA revealed heterogeneous glycoRNA distributions across six cell lines and an inverse correlation between FRET intensity and migratory capacity, with highly metastatic cells showing reduced FRET compared to cells with low migratory capacity. We further engineered sequence-specific proximity labeling using azide-modified in situ hybridization probes to recruit horseradish peroxidase, enabling covalent tagging of csRBPss. Quantitative proteomics identified 196 nanodomain-associated csRBPs enriched in focal adhesion and chemokine signaling. Collectively, GlycoFRET‑PLIA enables in situ visualization and interactome mapping of glycoRNAs, supporting their role as spatial organizers of prometastatic signalosomes.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":" ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149331694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Affinity Peptide-Engineered Magnetic Microswimmers for Ultrafast On-the-Fly Screening of Influenza A Virus in Microliter-Scale Samples. 亲和肽工程磁微游泳器在微升样品中超快速实时筛选甲型流感病毒。

IF 10.9 1区化学

ACS Sensors Pub Date : 2026-11-04 DOI: 10.1021/acssensors.6c03061

Alberto Rodríguez-Castillo, Pablo Del Barrio, Felipe Pérez-García, Harold Bermúdez-Marval, Beatriz Jurado-Sánchez, Alberto Escarpa

{"title":"Affinity Peptide-Engineered Magnetic Microswimmers for Ultrafast On-the-Fly Screening of Influenza A Virus in Microliter-Scale Samples.","authors":"Alberto Rodríguez-Castillo, Pablo Del Barrio, Felipe Pérez-García, Harold Bermúdez-Marval, Beatriz Jurado-Sánchez, Alberto Escarpa","doi":"10.1021/acssensors.6c03061","DOIUrl":"https://doi.org/10.1021/acssensors.6c03061","url":null,"abstract":"Monitoring respiratory viruses is crucial for mitigating their spread and the associated healthcare costs, especially during the cold season. This study presents an on-the-fly OFF-ON fluorescence approach for the H3N2 subtype of Influenza A virus determination using magnetically propelled WS2/Ni micromotors. The micromotors are modified with a rhodamine B-labeled peptide highly specific toward the hemagglutinin A HA1 protein antigen. The improved fluid mixing imparted by the micromotor motion allows for efficient operation during an assay time of 1 min using just 1 μL of sample. The developed fluorescent assay represents a reliable and robust screening platform for influenza clinical samples, achieving exceptional selectivity and limits of detection in the ng/L range. The method's performance was validated using nasopharyngeal specimens, demonstrating high reliability and analytical specificity for the Influenza A H3N2 subtype. Notably, the protocol provided high fluorescence signatures for H3N2-infected samples even when cycle threshold (Ct) values were comparable those of to H1N1-infected samples and negative samples. These results highlight the reliability of the proposed micromotor-based strategy for rapid viral subtyping and demonstrate its potential as a fast sample-to-result diagnostic platform requiring minimal sample volume.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":" ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149331692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multichannel Electronic Suture for Spatiotemporal Monitoring of Inflammatory Levels within Closed Wounds. 多通道电子缝线用于封闭伤口炎症水平的时空监测。

IF 10.9 1区化学

ACS Sensors Pub Date : 2026-11-04 DOI: 10.1021/acssensors.6c01212

Haodong Meng, Zhirong Liu, Kangdi Guan, Junhao Hu, Dan Kuang, Di Chen, Guozhen Shen

{"title":"Multichannel Electronic Suture for Spatiotemporal Monitoring of Inflammatory Levels within Closed Wounds.","authors":"Haodong Meng, Zhirong Liu, Kangdi Guan, Junhao Hu, Dan Kuang, Di Chen, Guozhen Shen","doi":"10.1021/acssensors.6c01212","DOIUrl":"https://doi.org/10.1021/acssensors.6c01212","url":null,"abstract":"Surgical sutures are indispensable in modern healthcare as the primary means of suturing tissues and facilitating healing. However, conventional sutures inherently obscure the visualization of underlying healing processes, creating a critical diagnostic blind spot for clinicians. Here, we present a flexible multichannel electronic (MultiE) suture for spatiotemporal monitoring of wound pH as a quantitative indicator of inflammatory dynamics within the closed wound bed. The MultiE suture features a coaxial architecture, comprising a central reference fiber electrode surrounded by multiple spirally arranged working electrodes functionalized with polyaniline. This design allows for the customization of diameter, length, as well as the number and distribution of sensing units to adapt to diverse clinical scenarios. In vitro studies demonstrated its stable pH monitoring over 17 days with an average super-Nernstian sensitivity of 80.71 mV pH-1. We further validated its spatiotemporal inflammatory assessment capability in rat models of normal wound and acute inflammation induction wound, without disrupting the wound healing process. This work presents an evolution of conventional sutures that can accurately localize inflammatory areas, showcasing their potential for post-closure wound management.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":" ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149331696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding Disease Drop by Drop. 解码疾病的点滴。

IF 10.9 1区化学

ACS Sensors Pub Date : 2026-11-03 DOI: 10.1021/acssensors.6c05144

Andrew J deMello

引用次数: 0

Quartz-on-Silicon Microacoustic Multiplexed Biosensor for In Vitro Label-Free Medical Diagnosis 用于体外无标签医学诊断的石英硅微声多路生物传感器

IF 8.9 1区化学

ACS Sensors Pub Date : 2026-11-03 DOI: 10.1021/acssensors.6c03406

Aleksander Oseev,Franck Chollet,Thomas Lecompte,Thérèse Leblois

{"title":"Quartz-on-Silicon Microacoustic Multiplexed Biosensor for In Vitro Label-Free Medical Diagnosis","authors":"Aleksander Oseev,Franck Chollet,Thomas Lecompte,Thérèse Leblois","doi":"10.1021/acssensors.6c03406","DOIUrl":"https://doi.org/10.1021/acssensors.6c03406","url":null,"abstract":"Advanced biomedical analyses require simultaneous quantification of multiple entities (cells, proteins, viruses, vesicles, …) calling for a multiplexed and compact biosensor. Label-free approaches giving access to the kinetic of interaction between key molecules are solid candidates for these analyses, but they currently lack either robustness for field use, multiplexing ability, or specificity. In this contribution, we report on the development of a new type of device that merges advantages of microsystem-based integrated solutions and performance of quartz acoustic wave biosensors. We have fabricated an acoustic wave biosensor composed of multiple independent detection units, each equipped with a specific surface biointerface for the selective detection and quantification of target analytes. The implementation of reflector structures on each sensing unit enables effective wave confinement, allowing for multiplexed measurements without compromising the performance of individual units. This technology relies on commercially available quartz and silicon wafers. To demonstrate the usefulness of our approach, we developed a proof-of-concept biosensor for the assessment of primary hemostasis. This assessment is based on monitoring the adhesion of platelets on functionalized surfaces during the sequence of events that leads to primary hemostasis. The biosensor directly takes whole blood and allows real-time measurement of resonance frequency and dissipation factor of 3 sensing units placed in a row. We have then characterized the specificity, repeatability, cross-talk, and sensitivity and estimated the resolution of the multiplexed biosensor. Finally, the device allowed detecting a simulated primary hemostasis defect using a modified blood sample. This proposed bioassay technology offers strong potential for broad applications in biomedical studies as an improved alternative to quartz crystal microbalance-based devices for multiplexed biointeraction analysis.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"108 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2026-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149305354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Entropy Engineering of Electronic Structure and Oxygen Regulation in LaMnO3 Perovskite for Total NOx Monitoring 用于总NOx监测的LaMnO3钙钛矿电子结构和氧调控的高熵工程

IF 8.9 1区化学

ACS Sensors Pub Date : 2026-11-03 DOI: 10.1021/acssensors.6c02474

Shutao Zhang,Yuehua Li,Tengteng Zhao,Weiwei Meng,Shangzhuo Wu,Ling Wang,Xu Li,Lei Dai

{"title":"High-Entropy Engineering of Electronic Structure and Oxygen Regulation in LaMnO3 Perovskite for Total NOx Monitoring","authors":"Shutao Zhang,Yuehua Li,Tengteng Zhao,Weiwei Meng,Shangzhuo Wu,Ling Wang,Xu Li,Lei Dai","doi":"10.1021/acssensors.6c02474","DOIUrl":"https://doi.org/10.1021/acssensors.6c02474","url":null,"abstract":"Accurate monitoring of total NOx remains challenging because NO2 reduction and NO oxidation involve different interfacial electrochemical processes, often leading to imbalanced responses. Herein, a Sr-assisted B-site high-entropy strategy was proposed to reconstruct the intrinsic electronic structure and surface defect chemistry of LaMnO3 (LMO) perovskite for impedance-metric YSZ based total NOx sensing. Sr doping coupled with Co/Cr/Fe/Mn/Ni high-entropy engineering establishes a coupled multivalent B-site redox network, optimizes the eg occupancy to 0.95, increases the density of states near the Fermi level, and modifies the surface oxygen environment. These electronic and surface-chemical changes enhance NOx adsorption and accelerate interfacial charge transfer at the triple-phase boundary (TPB). As a result, the La0.7Sr0.3(CoCrFeMnNi)0.2O3 (HE-LSMO) sensor exhibits enhanced responses to both NO2 and NO at 400 °C, with a 71% increase for NO2 and 55%/40% increases for NO in the 5–20 ppm and 20–120 ppm ranges, respectively. At 120 ppm, HE-LSMO delivers response values of 17.1° for NO2 and 16.3° for NO. More importantly, the sensor maintains a stable output under varying NO/NO2 ratios, O2 concentrations, humidity levels, and interfering gases, demonstrating its potential for reliable total NOx monitoring in complex exhaust environments.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"18 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2026-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149305355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Second-Generation JNK Activity Reporter Illuminates the Mechanism of AT1R-Induced JNK Activation 第二代JNK活性报告基因阐明了at1r诱导JNK激活的机制

IF 8.9 1区化学

ACS Sensors Pub Date : 2026-11-02 DOI: 10.1021/acssensors.6c02648

Daniel Stehle,Ben Li,Nwe Natalie Han,Nick David Bergkamp,Sohum Mehta,Jin Zhang

{"title":"Second-Generation JNK Activity Reporter Illuminates the Mechanism of AT1R-Induced JNK Activation","authors":"Daniel Stehle,Ben Li,Nwe Natalie Han,Nick David Bergkamp,Sohum Mehta,Jin Zhang","doi":"10.1021/acssensors.6c02648","DOIUrl":"https://doi.org/10.1021/acssensors.6c02648","url":null,"abstract":"c-Jun N-terminal kinases (JNKs) are central integrators of cell stress and are responsible for a multitude of signaling and gene expression changes in response to various environmental cues. Here, we describe the development of JNKAR2, a second-generation FRET-based JNK biosensor. JNKAR2 exhibits a ∼3.9-fold higher response amplitude over JNKAR1 while maintaining robust response dynamics and selectivity. Using JNKAR2 to illuminate JNK activation driven by angiotensin II (Ang II)/Ang II receptor type 1 (AT1R) signaling in HEK293 cells, we found that AT1R-mediated JNK activation is independent of β-arrestin but requires Gαq/11. Supporting a mechanism of Gαq/11-mediated JNK activation downstream of G protein-coupled receptors, stimulating the Gαq/11 coupling muscarinic acetylcholine M5 and M3 as well as endothelin type B receptors also induced robust JNKAR2 responses. On the other hand, neither the Gαi/o-preferring μ-opioid receptor nor the Gαs-preferring β2-adrenergic receptor was able to support JNK activation. The improved dynamic range of JNKAR2 further enabled us to probe Ang II-induced JNK signals in primary vascular smooth muscle cells, which were abolished by Gαq/11 inhibition. Targeting JNKAR2 to various subcellular compartments revealed that anisomycin induced global JNK signals, while AT1R-mediated JNK activation was absent from the plasma membrane and early endosomes. Together, we report on a second-generation JNK biosensor with substantially improved response amplitude that enables sensitive and selective visualization of JNK activation in real time and with subcellular resolution. JNKAR2 facilitates the dissection of the molecular mechanisms underlying JNK signaling.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"18 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2026-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149304438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0