ACS Sensors最新文献_第4页

Nitrogen-Doped Carbon Quantum Dots Activated Dandelion-Like Hierarchical WO₃ for Highly Sensitive and Selective MEMS Sensors in Diabetes Detection. 氮掺杂碳量子点激活蒲公英状分层 WO3，用于糖尿病检测中的高灵敏度和选择性 MEMS 传感器。

IF 8.2 1区化学

ACS Sensors Pub Date : 2025-02-28 Epub Date: 2024-10-04 DOI: 10.1021/acssensors.4c01840

Tianjun Ni, Zhonghu Dong, Kejie Xi, Yijia Lu, Kaiwen Chang, Chunpo Ge, Dong Liu, Zhijun Yang, Haijie Cai, Yongheng Zhu

{"title":"Nitrogen-Doped Carbon Quantum Dots Activated Dandelion-Like Hierarchical WO3 for Highly Sensitive and Selective MEMS Sensors in Diabetes Detection.","authors":"Tianjun Ni, Zhonghu Dong, Kejie Xi, Yijia Lu, Kaiwen Chang, Chunpo Ge, Dong Liu, Zhijun Yang, Haijie Cai, Yongheng Zhu","doi":"10.1021/acssensors.4c01840","DOIUrl":"10.1021/acssensors.4c01840","url":null,"abstract":"High sensitivity, low concentration, and excellent selectivity are pronounced primary challenges for semiconductor gas sensors to monitor acetone from exhaled breath. In this study, nitrogen-doped carbon quantum dots (N-CQDs) with high reactivity were used to activate dandelion-like hierarchical tungsten oxide (WO3) microspheres to construct an efficient and stable acetone gas sensor. Benefiting from the synergistic effect of both the abundant active sites provided by the unique dandelion-like hierarchical structure and the high reaction potential generated by the sensitization of the N-CQDs, the resulting 16 wt % N-CQDs/WO3 sensor shows an ultrahigh response value (Ra/Rg = 74@1 ppm acetone), low detection limit (0.05 ppm), outstanding selectivity, and reliable stability to acetone at the optimum working temperature of 210 °C. Noteworthy that the N-CQDs facilitate the carrier migration and intensify the reaction between acetone and WO3 during the sensing process. Considering the above advantages, N-CQDs as a sensitizer to achieve excellent gas-sensitive properties of WO3 are a promising new strategy for achieving accurate acetone detection in real time and facilitating the development of portable human-exhaled gas sensors.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":" ","pages":"699-708"},"PeriodicalIF":8.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142374701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas13a-Programmed Cu NCs and Z-Scheme T-COF/Ag₂S for Photoelectrochemical Biosensing of circRNA.

IF 8.2 1区化学

ACS Sensors Pub Date : 2025-02-28 Epub Date: 2025-01-31 DOI: 10.1021/acssensors.4c03180

Xinxin Yuan, Wenchao Geng, Jiangying Ji, Zhiyi Yan, Jiarui Wei, Yongjun Wu, Ruiying Yang

{"title":"CRISPR/Cas13a-Programmed Cu NCs and Z-Scheme T-COF/Ag2S for Photoelectrochemical Biosensing of circRNA.","authors":"Xinxin Yuan, Wenchao Geng, Jiangying Ji, Zhiyi Yan, Jiarui Wei, Yongjun Wu, Ruiying Yang","doi":"10.1021/acssensors.4c03180","DOIUrl":"10.1021/acssensors.4c03180","url":null,"abstract":"Circular RNAs (circRNAs), as a class of noncoding RNA molecules with a circular structure exhibit high stability and spatiotemporal-specific expression, making them ideal cancer biomarkers for liquid biopsy. Herein, a new photoelectrochemical (PEC) biosensor for a highly sensitive circRNA assay in the whole blood of lung cancer patients was designed based on CRISPR/Cas13a-programmed Cu nanoclusters (Cu NCs) and a Z-scheme covalent organic framework/silver sulfide (T-COF/Ag2S) composite. This Z-scheme T-COF/Ag2S composite accelerates electron transfer and produces an excellent initial photocurrent. When CRISPR/Cas13a precisely targets circRNA, it nonspecifically cleaves the triple-helix molecular structure to release DNA fragments (C'/C\"). After the C'/C\" opens the DNA hairpin probe (HP) modified on the electrode, hybridization chain reactions are performed to produce abundant AT-rich double-stranded DNA with the addition of H1 and H2 probes. Upon the incubation of Cu2+, Cu NCs are in situ formed via the A-Cu2+-T bonds and can effectively quench the photocurrent of the Z-scheme T-COF/Ag2S due to the energy transfer process. This developed PEC biosensor for the circRNA assay shows a low limit of detection of 0.5 fM, and the reusability of DNA-modified magnetic beads (MB-DNA) reduces the detection cost. Moreover, the PEC biosensor can accurately quantify the circRNA level and distinguish the circRNA expression in whole blood from healthy controls and lung cancer patients, offering strong potential in clinical diagnosis.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":" ","pages":"1270-1279"},"PeriodicalIF":8.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid and Sensitive Escherichia coli Detection: Integration of SERS and Acoustofluidics in a Lysis-Free Microfluidic Platform.

IF 8.2 1区化学

ACS Sensors Pub Date : 2025-02-28 Epub Date: 2025-01-30 DOI: 10.1021/acssensors.4c03118

Sohyun Park, Kihyun Kim, Anna Go, Min-Ho Lee, Lingxin Chen, Jaebum Choo

{"title":"Rapid and Sensitive Escherichia coli Detection: Integration of SERS and Acoustofluidics in a Lysis-Free Microfluidic Platform.","authors":"Sohyun Park, Kihyun Kim, Anna Go, Min-Ho Lee, Lingxin Chen, Jaebum Choo","doi":"10.1021/acssensors.4c03118","DOIUrl":"10.1021/acssensors.4c03118","url":null,"abstract":"Bacterial infections, such as sepsis, require prompt and precise identification of the causative bacteria for appropriate antibiotics treatment. Traditional methods such as culturing take 2-5 days, while newer techniques such as reverse transcription-polymerase chain reaction and mass spectrometry are hindered by blood impurities. Consequently, this study developed a surface-enhanced Raman scattering (SERS)-based acoustofluidic technique for rapid bacterial detection without culturing or lysing. Target bacteria are first tagged with SERS nanotags in a microtube. The solution with tagged bacteria and unbound SERS nanotags is passed through a silicon microfluidic channel. A piezoelectric transducer generates acoustic waves within the channel, concentrating larger tagged bacteria in the center and pushing smaller unbound nanotags toward the channel walls. A laser beam is focused at the center of the channel, and the Raman signals of bacteria passing through the focal volume are measured for quantitative analysis. As a proof of concept, this study detected various concentrations of Escherichia coli at a limit of detection of 1.75 × 105 CFU/mL within 1 h. This method offers significant clinical potential, enabling rapid and accurate bacterial identification without genetic material extraction, cultivation, or lysis.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":" ","pages":"1217-1227"},"PeriodicalIF":8.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solution-Gated Thin Film Transistor Biosensor-Based SnO₂ Amorphous Film for Label-Free Detection of Epithelial Cell Adhesion Molecules.

IF 8.2 1区化学

ACS Sensors Pub Date : 2025-02-28 Epub Date: 2025-01-31 DOI: 10.1021/acssensors.4c03073

Yaxing Zhang, Zhiwei Cai, Rong Zou, Ruling Wang, Runan Tan, Lei Wang, Yuxiang Wu, Hanping He, Yunbin He, Gang Chang

{"title":"Solution-Gated Thin Film Transistor Biosensor-Based SnO2 Amorphous Film for Label-Free Detection of Epithelial Cell Adhesion Molecules.","authors":"Yaxing Zhang, Zhiwei Cai, Rong Zou, Ruling Wang, Runan Tan, Lei Wang, Yuxiang Wu, Hanping He, Yunbin He, Gang Chang","doi":"10.1021/acssensors.4c03073","DOIUrl":"10.1021/acssensors.4c03073","url":null,"abstract":"Epithelial cell adhesion molecule (EpCAM) was considered to be an important marker of multiple tumors, and its high expression is closely related to the early diagnosis and treatment of tumors. At present, metal oxide semiconductors have become a key component of biosensor and bioelectronics technology. Tin oxide shows great potential for development because of its nontoxic, nonpolluting, low price, and excellent electrical properties. In this study, a novel SnO2 solution-gated thin film transistor (SGTFT) biosensor for the specific detection of EpCAM was successfully developed using SnO2 film prepared by the sol-gel method as the channel material. By selecting the optimal thickness of 100 nm SnO2 film as the channel material, the transconductance value (gm) reached 1432 μS, and the threshold voltage (Vth) remained stable at 0.288 V. In order to achieve qualitative and quantitative detection of EpCAM, SnO2 films were subjected to a specific chemical treatment to fix the aptamer. Through a specific recognition between the aptamer and EpCAM, the gate voltage changes were triggered to regulate the channel current of the device. FE-SEM, EIS, XPS, and electrical performance tests were employed to track and measure the modification process. Based on the optimizations described above, the prepared SGTFT exhibited high detection sensitivity (14.6 mV·dec-1), the limit of detection (LOD) down to 24.4 pg/mL, and the calibration curves in the range of 0.02 ng/mL-500 ng/mL for EpCAM sensing. The developed SnO2-SGTFT biosensor is anticipated to provide a new highly sensitive and specific detection platform for health monitoring and disease diagnosis.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":" ","pages":"1187-1196"},"PeriodicalIF":8.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probiotic Antimicrobial Evaluation Via Real-Time Profiling of Bacterial Cell Proliferation Using Stochastic Kinetics

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-02-27 DOI: 10.1021/acssensors.4c03003

Seong-Geun Jeong, Youjin Lee, Hye-Seon Jeong, Seong Jun Park, Jinki Yeom, Chang-Hyung Choi, Byung-Gee Kim

{"title":"Probiotic Antimicrobial Evaluation Via Real-Time Profiling of Bacterial Cell Proliferation Using Stochastic Kinetics","authors":"Seong-Geun Jeong, Youjin Lee, Hye-Seon Jeong, Seong Jun Park, Jinki Yeom, Chang-Hyung Choi, Byung-Gee Kim","doi":"10.1021/acssensors.4c03003","DOIUrl":"https://doi.org/10.1021/acssensors.4c03003","url":null,"abstract":"Probiotic metabolites are gaining attention as potential antibiotic candidates against antibiotic-resistant bacteria. The disk diffusion test, by measuring bacterial aggregate responses, faces challenges in accurately evaluating antimicrobial efficacy when these responses to different probiotic strains are indistinguishable at a macroscopic level. Here, this study presents an analytical method for accurately evaluating antimicrobial activity by analyzing bacterial cell proliferation suppression at a microscopic level. This assay can be used in a coculture system, designed to continuously expose pathogenic bacteria growing on the bottom surface of the culture plate to probiotic metabolites, selectively released from porous capsules positioned above. Bacterial proliferation is optically monitored in real-time and tracked via a computer vision algorithm. Specifically, bacterial proliferation is quantified as their doubling time, calculated using a proposed stochastic kinetic model. This method identifies the most potent antimicrobial strains by determining which probiotic candidates most effectively extend the bacterial doubling time. In comparative experiments using the same strains, this proposed method demonstrated clear distinctions in the antimicrobial efficacy of each strain, unlike the disk diffusion test. Therefore, this approach provides a reliable solution for identifying superior probiotic strains, with potential for widespread use in discovering new antimicrobial agents.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"51 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143506823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ant-Inspired Ion Gel Sensor for Dual-Mode Detection of Force and Humidity via Magnetic Induction

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-02-27 DOI: 10.1021/acssensors.5c00032

Boyi Xu, Jinsui Xu, Haoran Ou, Feihu Song, Zheping Wang, Ye Tian, Yifan Lu, Zhangqing Duan, Fei Yang, Yitong Zhou

{"title":"Ant-Inspired Ion Gel Sensor for Dual-Mode Detection of Force and Humidity via Magnetic Induction","authors":"Boyi Xu, Jinsui Xu, Haoran Ou, Feihu Song, Zheping Wang, Ye Tian, Yifan Lu, Zhangqing Duan, Fei Yang, Yitong Zhou","doi":"10.1021/acssensors.5c00032","DOIUrl":"https://doi.org/10.1021/acssensors.5c00032","url":null,"abstract":"In the realm of intelligent sensing, the development of multifunctional, highly sensitive, wide-ranging, and durable flexible sensors remains a formidable challenge. This paper introduces a dual-mode ionic gel sensor, inspired by the sensory mechanisms of ants and developed through magnetically induced technology capable of simultaneously detecting environmental humidity and pressure. The humidity sensor is fabricated from a prestretched, buckled poly(vinyl alcohol) (PVA) ionic gel with microstructures, exhibiting rapid response and high durability. The voltage signal of the humidity sensor decreases linearly with increasing relative humidity (RH). The pressure sensor employs a capacitive structure, integrating magnetically induced fiber pilose structures with ionic gel, achieving a high sensitivity of 7.375 kPa–1 and an excellent linear response over a broad pressure range from 0.5 Pa to 95 kPa. In the context of human motion monitoring, the sensor accurately captures physiological signals, such as pulse beats and joint movements while concurrently detecting skin humidity. Additionally, by leveraging deep learning algorithms, the sensor attains a remarkable 99.21% accuracy in object recognition within flexible intelligent sorting systems, underscoring its potential in smart logistics sorting applications. This study transcends the traditional limitations of capacitive pressure sensors regarding sensitivity and detection range, offering novel solutions for applications in motion monitoring and intelligent logistics sorting.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"27 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143518582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Headband for Monitoring Chloride Anions in Sweat Using Developed Flexible Patches

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-02-27 DOI: 10.1021/acssensors.4c03366

Chen-Lu Wang, Xin Cai, Yong-Huan Zhao, Zi-Hao Liu, Rui-Ze Xia, Li-Jun Tang, Zong-Yin Song, Shi-Hua Chen, Yixiang Li, Meng Yang, Pei-Hua Li, Xing-Jiu Huang

{"title":"Integrated Headband for Monitoring Chloride Anions in Sweat Using Developed Flexible Patches","authors":"Chen-Lu Wang, Xin Cai, Yong-Huan Zhao, Zi-Hao Liu, Rui-Ze Xia, Li-Jun Tang, Zong-Yin Song, Shi-Hua Chen, Yixiang Li, Meng Yang, Pei-Hua Li, Xing-Jiu Huang","doi":"10.1021/acssensors.4c03366","DOIUrl":"https://doi.org/10.1021/acssensors.4c03366","url":null,"abstract":"Flexible wearable potentiometric ion sensors for continuous monitoring of electrolyte cations have made significant advances in bioanalysis for personal healthcare and diagnostics. However, less attention is paid to the most abundant extracellular anion, chloride ion (Cl–) as a mark of electrolyte imbalance and an important diagnostic indicator of cystic fibrosis, which has important significance for accurate monitoring in complex biological fluids. An all-solid-state Cl–-selective electrode is constructed utilizing oxygen vacancies reinforced vanadium oxide with a nitrogen-doped carbon shield as the solid contact (V2O3–x@NC/Cl–-ISE). The prepared V2O3–x@NC/Cl–-ISE exhibits a low detection limit of 10–5.45 M without an interfacial water layer and shows a highly stable potential with 7.24 μV/h during 24 h, which is attributed to the rapid interfacial electron transfer of the conductive carbon layers and the valence state transition of the polyvalent vanadium center in charge storage processes. Additionally, the custom flexible sensing patch presents an excellent sensitivity retention rate under bending (95%) and twisting (93%) strains and possesses good anti-interference performance (ΔE < 8 mV) against common interfering ions and organic substances in sweat. Real-time monitoring of the Cl– concentration in sweat aligns with ion chromatography analysis results. This study presents a compact wearable Cl– monitoring platform for the easy tracking of exercise-induced dehydration and cystic fibrosis screening with promising applications in smart healthcare.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"52 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143518581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing a Fluorescent Nucleobase Surrogate for Supramolecular FRET-Aptamer Detection and Target-Site Mapping

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-02-26 DOI: 10.1021/acssensors.4c02772

Karley J. L. Zimmer, Ryan E. Johnson, Hunter Little, Jean Duhamel, Richard A. Manderville

{"title":"Harnessing a Fluorescent Nucleobase Surrogate for Supramolecular FRET-Aptamer Detection and Target-Site Mapping","authors":"Karley J. L. Zimmer, Ryan E. Johnson, Hunter Little, Jean Duhamel, Richard A. Manderville","doi":"10.1021/acssensors.4c02772","DOIUrl":"https://doi.org/10.1021/acssensors.4c02772","url":null,"abstract":"DNA aptamers can bind small molecule ligands with high affinity and specificity to produce a unique supramolecular structure. Methods to obtain structural information about the binding interaction coupled with sensitive diagnostics is a gold standard for aptasensor design. However, most sensing strategies afford ligand detection without structural insight, while NMR- or crystallography-based structural methods lack sensitivity required for diagnostics. FRET-based strategies can afford both, especially with internal fluorescent nucleobase probes that are spatially fixed within the helix, but dual aptamer labeling can compromise aptamer affinity toward its target. Herein, we showcase a nucleobase surrogate-ligand FRET-based strategy that affords target-site mapping combined with sensitive target detection that addresses these challenges. A fluorescent molecular rotor (FMR) thiophene chalcone (Th6HI) nucleobase surrogate was incorporated into the tetracycline (TC) 42-mer DNA binding aptamer OTC2 to serve as an acceptor for the TC donor. Time-resolved fluorescence anisotropy experiments predict a compact prefolded OTC2 aptamer that is hardly impacted by TC binding. Consequently, direct excitation of the internal FMR Th6HI at 530 nm affords little response to TC binding, as probe rigidity is not strongly altered. In contrast, indirect excitation of the Th6HI probe through TC donor excitation at 378 nm affords site-dependent sensitized fluorescence (Fsen) of the Th6HI acceptor to afford enhanced sensitivity for TC detection compared to a native platform, which utilizes the intrinsic TC fluorescence. Furthermore, the FRET response provides target-site mapping to build a new binding model for the TC-OTC2 complex that is akin to the three-helical structure of the hammerhead ribozyme.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"17 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143496134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Arterial Stenosis Based on Synchronized Signals from Wearable Pulse and Blood Flow Velocity Sensors

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-02-25 DOI: 10.1021/acssensors.4c03537

Pengrui Zhu, Xiaowei Zhao, Xuanhe Chen, Ran Liu, Han Ouyang, Yiran Hu, Bojing Shi, Yubo Fan

{"title":"Detection of Arterial Stenosis Based on Synchronized Signals from Wearable Pulse and Blood Flow Velocity Sensors","authors":"Pengrui Zhu, Xiaowei Zhao, Xuanhe Chen, Ran Liu, Han Ouyang, Yiran Hu, Bojing Shi, Yubo Fan","doi":"10.1021/acssensors.4c03537","DOIUrl":"https://doi.org/10.1021/acssensors.4c03537","url":null,"abstract":"Atherosclerosis is the main cause of ischemic stroke. It occurs as a condition that leads to thickening of the arterial blood vessel walls and narrowing of the blood vessels, which can seriously affect the normal flow of blood. Currently, the detection of arterial stenosis relies on large-scale hospital equipment like computed tomography (CT) and magnetic resonance imaging (MRI), which require specialized technicians to operate and are not convenient for daily use. In addition, stenosis affects multiple parameters of hemodynamics in the blood flow field, and relying on a single physical quantity is not sufficient to understand the blood flow field localized in the stenotic vessel. Here, we demonstrated combined sensors of pulse wave and blood flow velocity (CSPB) based on photoelectric plethysmography and an ultrasonic Doppler device. We found that when the stenosis rate increased by 30%, the amplitude difference of the pulse wave curve between the two sides of the stenosis increased by over 11%, the amplitude of the blood flow curve decreased by 8%, and the blood flow resistance increased by 11%. We also prepared silicone-based models of blood stenosis vessels to build in vitro blood flow systems and achieve more accurate simulation of vascular stenosis diseases. Based on this, we studied the pulse wave and blood flow velocity curves of CSPB under different stenosis parameters. Meanwhile, we used the finite element analysis method of fluid–structure interactions to study the pulse wave and blood flow velocity changes under different arterial stenosis conditions. This study is expected to provide theoretical and technical references for achieving noninvasive detection of cardiovascular and cerebrovascular diseases based on multisensor fusion.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"42 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143496137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tracking Globally 5-Methylcytosine and Its Oxidized Derivatives in Colorectal Cancer Epigenome Using Bioelectroanalytical Technologies

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-02-25 DOI: 10.1021/acssensors.4c03290

Eloy Povedano, Víctor Pérez-Ginés, Rebeca M. Torrente-Rodríguez, Raquel Rejas-González, Ana Montero-Calle, Alberto Peláez-García, Jaime Feliú, María Pedrero, José M. Pingarrón, Rodrigo Barderas, Susana Campuzano

{"title":"Tracking Globally 5-Methylcytosine and Its Oxidized Derivatives in Colorectal Cancer Epigenome Using Bioelectroanalytical Technologies","authors":"Eloy Povedano, Víctor Pérez-Ginés, Rebeca M. Torrente-Rodríguez, Raquel Rejas-González, Ana Montero-Calle, Alberto Peláez-García, Jaime Feliú, María Pedrero, José M. Pingarrón, Rodrigo Barderas, Susana Campuzano","doi":"10.1021/acssensors.4c03290","DOIUrl":"https://doi.org/10.1021/acssensors.4c03290","url":null,"abstract":"This work presents the first electroanalytical bioplatforms to track individually or simultaneously at a global level all four methylation marks involved in the DNA methylation–demethylation cycle: 5-methylcytosine (5mC) and their sequential oxidative derivatives (5-hydroxymethyl-(5hmC), 5-formyl-(5fC), and 5-carboxyl-(5caC) cytosines). The bioplatforms employed direct competitive immunoassay formats implemented on the surface of magnetic microparticles (MBs) and involved capture antibodies specific to each epimark as well as synthetic biotinylated DNA oligomers with a single epimark that were enzymatically marked with horseradish peroxidase (HRP) to perform an amperometric readout on disposable platforms for single or multiplexed detection. These new electroanalytical biotechnologies, groundbreaking from analytical and clinical perspectives, achieved attractive operational characteristics, reaching detection limits at pM levels for synthetic single epimark-bearing DNA oligomers. The developed methodology was applied to track globally all four target epimarks in a fast, simple, sensitive, and selective way while their correlation in genomic DNA extracted from paired healthy and tumor tissues of patients with colorectal cancer (CRC) was established for the first time.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"1 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143496141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0