ACS Sensors最新文献

{"title":"Ultrasensitive Electrochemical Vertical Flow Immunoassay for Rapid and Simultaneous Detection of Interleukin-6 and Procalcitonin","authors":"Xudong Ying, Wenxuan Fu, Min Qi, Lin Zhou, Wei Li, Bin Su","doi":"10.1021/acssensors.4c02257","DOIUrl":"https://doi.org/10.1021/acssensors.4c02257","url":null,"abstract":"Rapid and multiplexed detection of biomarkers plays an indispensable role in disease diagnosis. Although paper-based lateral flow immunoassays have been widely used in this field, the speediness and throughput are still challenging issues. Herein, an electrochemical vertical flow immunoassay device (eVFID) is fabricated for rapid, ultrasensitive, and multiplexed detection of inflammatory biomarkers. Working electrodes with excellent electrochemical performance and permeability properties were directly fabricated on the nitrocellulose membrane to enable both the vertical flow of the sample solution and electrochemical detection. This vertical configuration can remarkably improve the speediness of the immunoassay and effectively inhibit the cross-talk reactions among immunomolecules, thus allowing rapid and simultaneous detection of multiplexed biomarkers. Furthermore, a signal amplification strategy based on horseradish peroxidase and tetramethylbenzidine was integrated into the eVFID to substantially increase the sensitivity of the electrochemical detection. A low limit of detection of 0.1 and 0.22 pg mL^–1 was obtained for two low-abundance inflammatory biomarkers, interleukin-6 (IL-6) and procalcitonin (PCT), respectively. Finally, using a two-channel eVFID, simultaneous detection of IL-6 and PCT in human plasma samples was successfully realized within 5 min. We believe that the eVFID holds great promise for speedy and high-throughput biomarker detection at the point of care.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"4 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tandem Imaging of Breath Ethanol and Acetaldehyde Based on Multiwavelength Enzymatic Biofluorometry","authors":"Kenta Iitani, Rintaro Miura, Jihu Lim, Ryotaro Ishida, Kenta Ichikawa, Koji Toma, Takahiro Arakawa, Kohji Mitsubayashi","doi":"10.1021/acssensors.4c02451","DOIUrl":"https://doi.org/10.1021/acssensors.4c02451","url":null,"abstract":"Highly sensitive and selective imaging of human-borne volatile organic compounds (VOCs) enables an intuitive understanding of their concentrations and release sites. While multi-VOC imaging methods have the potential to facilitate step-by-step metabolic tracking and improve disease screening accuracy, no such system currently exists. In this study, we achieved simultaneous imaging of ethanol (EtOH) and acetaldehyde (AcH), the starting molecule and an intermediate metabolite of alcohol metabolism, using a multiwavelength VOC imaging system. The system employed alcohol dehydrogenase-catalyzed substrate oxidation (ADH_OX) and reduction (ADH_RD) reactions. The oxidation of EtOH by ADH_OX in the presence of NAD⁺ produced NADH, which was subsequently oxidized by diaphorase (DP) with resazurin, leading to the resorufin formation, characterized by red fluorescence (excitation at 560 nm and fluorescence at 590 nm). Reduction of AcH by ADH_RD consumed NADH, leading to a decrease in blue fluorescence (ex. 340 nm, fl. 490 nm). Meshes incorporating ADH_OX-DP or ADH_RD were arranged in tandem in front of a camera. Fluorescence images were captured, while a mixture of gaseous EtOH and AcH was applied by switching between two bandpass filters at 1 Hz. Each mesh exhibited selective responses to the target VOCs, with no significant impact on the dynamic range observed in either the single or tandem configurations (EtOH 1–300 ppm, AcH 0.2–5 ppm). The 90% response time was close after time–domain image differential analysis (EtOH = 26 s and AcH = 15 s). Furthermore, the system enabled simultaneous and quantitative imaging of EtOH and AcH concentrations in the breath after alcohol consumption. It also distinguished differences in alcohol metabolism based on the alcohol dehydrogenase 2 (ALDH2) activity, as indicated by the EtOH/AcH ratio (ALDH2 active vs nonactive: 120.9/0.71 ppm vs 129.2/1.99 ppm).","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"4 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142776831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly Sensitive Ethylene Glycol Gas Sensor Based on MIL-68(In)@ZIF-8 Derivative","authors":"Huirong Kou, Tingting Shao, Juntang Dong, Fuchun Zhang, Shuwei Tian, Xiaoyang Wang","doi":"10.1021/acssensors.4c02087","DOIUrl":"https://doi.org/10.1021/acssensors.4c02087","url":null,"abstract":"Ethylene glycol, as a colorless and tasteless organic compound, is an important industrial raw material but can be hazardous to the environment and human health. Thus, the development of high-performance sensing materials is required for the monitoring of ethylene glycol. In this paper, a method to synthesize In₂O₃@ZnO using MIL-68(In)@ZIF-8 to serve as a sacrificial template is proposed for testing ethylene glycol sensing capabilities. For verifying an effective improvement in gas-sensitive performance by bimetallic organic skeleton (MOF) synthesized heterojunctions, we performed gas-sensitive tests on In₂O₃, ZnO, and In₂O₃@ZnO. In₂O₃@ZnO has the best sensitivity to ethylene glycol, including ultrahigh response value (20 ppm-200.12), moderate response/recovery time (53/50 s), and excellent selectivity. The construction of heterojunction is the main reason for enhancing the ethylene glycol response of the sensor. On this basis, the gas-sensitive enhancement mechanism of composites is analyzed. The results show that the design method of synthesizing heterojunctions using bis-MOFs proposes a new approach that enhances the properties of ethylene glycol.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"246 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scalable Photonic Nose Development through Corona Phase Molecular Recognition","authors":"Minyeong Yoon, Seyoung Shin, Seungju Lee, Joohoon Kang, Xun Gong, Soo-Yeon Cho","doi":"10.1021/acssensors.4c02327","DOIUrl":"https://doi.org/10.1021/acssensors.4c02327","url":null,"abstract":"Breath sensors promise early disease diagnosis through noninvasive, rapid analysis, but have struggled to reach clinical use due to challenges in scalability and multivariate data extraction. The current breath sensor design necessitates various channel materials and surface functionalization methods, which delays the process. Additionally, the limited options for channel materials that provide optimum sensitivity and selectivity further restrict the array size to a maximum of only 10 to 20 channels. To address these limitations, we propose a breath sensing array design process based on Corona Phase Molecular Recognition (CoPhMoRe), which enables the creation of an expansive library of nanoparticle interfaces and broad fingerprints for multiple analytes in the breath. Although CoPhMoRe has predominantly been utilized for liquid-phase sensing, its recent application to gas-phase sensing has shown significant potential for breath sensing. We introduce the recent demonstrations in the field and present the concept of a CoPhMoRe-based photonic-nose sensor array, leveraging fluorescent nanomaterials such as near-infrared single-walled carbon nanotubes. Additionally, we identified four critical milestones for translating CoPhMoRe into breath sensors for practical clinical applications.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"15 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142776833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pulse Ultrasound-Based Response Enhancement of a MOX Gas Sensor","authors":"Yumin Yang, Junhui Hu","doi":"10.1021/acssensors.4c01548","DOIUrl":"https://doi.org/10.1021/acssensors.4c01548","url":null,"abstract":"In this work, a new method to enhance the sensing response of an ultrasonically catalyzed metal oxide gas sensor has been proposed and developed, in which pulse ultrasound is employed to enhance the redox reaction at the sensing surface. It is experimentally confirmed that with a proper pulse width, the negative effect of acoustic streaming on the ultrasonic enhancement process can be effectively suppressed. Comparing the steady responses of five target gases under the pulse and continuous ultrasound, respectively, it is found that the pulse ultrasound causes a better catalysis effect, and response enhancement (RE) by the pulse ultrasound with an optimal pulse width depends on the ultrasonic strength as well as the species and concentration of the target gas. For 2 ppm methanol, the RE by the pulse ultrasound is 50%, relative to the continuous ultrasound, when the pulse width, duty ratio, and working frequency are 0.4 ms, 50%, and 110.1 kHz, respectively. The optimal pulse width decreases when the ultrasonic strength increases and is not affected by the target gas species and concentration. The lower the target gas concentration, the larger the optimal RE caused by pulse ultrasound. Moreover, for a given pulse width, the interpulse time also affects the RE.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"137 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142776832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fully Automated and AI-Assisted Optical Fiber Sensing System for Multiplexed and Continuous Brain Monitoring","authors":"Yuqian Zhang, Naihan Zhang, Yubing Hu, Christopher Pereira, Michael Fertleman, Nan Jiang, Ali K. Yetisen","doi":"10.1021/acssensors.4c02126","DOIUrl":"https://doi.org/10.1021/acssensors.4c02126","url":null,"abstract":"Continuous and comprehensive brain monitoring is crucial for timely identification of changes or deterioration in brain function, enabling prompt intervention and personalized treatments. However, existing brain monitoring systems struggle to offer continuous and accurate monitoring of multiple brain biomarkers simultaneously. This study introduces a multiplexed optical fiber sensing system for continuous and simultaneous monitoring of six cerebrospinal fluid (CSF) biomarkers using tip-functionalized optical fibers and computational algorithms. Optimized machine learning models are developed and integrated for real-time spectra analysis, allowing for precise and continuous readout of biomarker concentrations. The developed machine learning-assisted fiber optic sensing system exhibits high sensitivity (0.04, 0.38, 0.67, 2.62, 0.0064, 0.33 <i>I</i>/<i>I</i>₀ change per units of temperature, dissolved oxygen, glucose, pH, Na⁺, Ca²⁺, respectively), reversibility, and selectivity toward target biomarkers with a total diameter less than 2.5 mm. By monitoring brain metabolic and ionic dynamics, this system accurately identified brain physiology deterioration and recovery using <i>ex vivo</i> traumatic brain injury models. Additionally, the system successfully tracked biomarker fluctuations in clinical CSF samples with high accuracy (<i>R</i>² &gt; 0.93), demonstrating excellent sensitivity and selectivity in reflecting disease progression in real time. These findings underscore the enormous potential of automated and multiplexed optical fiber sensing systems for intraoperative and postoperative monitoring of brain physiologies.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"37 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142763043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ferrocenyl Compounds as Alternative Redox Labels for Robust and Versatile Electrochemical Aptamer-Based Sensors","authors":"Gyeongho Kim, Soo Eun Park, Woohyeong Lee, Jung Min Joo, Haesik Yang","doi":"10.1021/acssensors.4c01773","DOIUrl":"https://doi.org/10.1021/acssensors.4c01773","url":null,"abstract":"This study explores the potential of seven ferrocenyl (Fc) compounds with cross-linking groups as alternative redox labels to methylene blue (MB) for electrochemical aptamer-based (E-AB) sensors. The cross-linking efficiency, formal potential (<i>E</i>⁰′), and electrochemical durability of these compounds were evaluated. Compound <b>Fc1a-X</b> exhibited superior performance, characterized by efficient cross-linking, a moderate and pH-insensitive <i>E</i>⁰′, and enhanced durability during repeated potential scans. The attachment of <b>Fc1a-X</b>, which includes a 3-carbon chain spacer and an <i>N</i>-hydroxysuccinimide-ester cross-linking group, to an amine-terminated monolayer on a Au electrode demonstrated high cross-linking efficiency, which is critical for achieving high sensitivity. The <i>E</i>⁰′ of <b>Fc1a-X</b> attached to the aptamer monolayer was 0.14 V, which is within the optimal range of −0.2 to 0.2 V vs Ag/AgCl. Square wave voltammetry showed that the peak potential and current of <b>Fc1a-X</b> are pH-insensitive, which is critical for versatile use. In serum, <b>Fc1a-X</b> maintained stable peak current levels without a gradual decrease after an initial rapid decrease during the first 2 h with considerably less reduction over 12 h compared to MB. Using <b>Fc1a-X</b> as the redox label, an E-AB sensor effectively detected doxorubicin in serum, covering the clinical range. These findings suggest <b>Fc1a-X</b> as a promising candidate for developing robust, versatile, and sensitive E-AB sensors.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"19 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142763044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering an Ultrafast Ambient NO2 Gas Sensor Using Cotton-Modified LaFeO3/MXene Composites","authors":"Neeraj Dhariwal, Preety Yadav, Manju Kumari, Akanksha, Amit Sanger, Sung Bum Kang, Vinod Kumar, Om Prakash Thakur","doi":"10.1021/acssensors.4c02597","DOIUrl":"https://doi.org/10.1021/acssensors.4c02597","url":null,"abstract":"This work presents a room-temperature (RT) NO₂ gas sensor based on cotton-modified LaFeO₃ (CLFO) combined with MXene. LaFeO₃ (LFO), CLFO, and CLFO/MXene composites were synthesized via a hydrothermal method. The fabricated sensor, utilizing MXene/CLFO, exhibits a p-type behavior and fully recoverable sensing capabilities for low concentrations of NO₂, achieving a higher response of 14.2 times at 5 ppm. The sensor demonstrates excellent performance with a response time of 2.7 s and a recovery time of 6.2 s, along with notable stability. The sensor’s sensitivity is attributed to gas interactions on the material’s surface, adsorption energy, and charge-transfer mechanisms. Techniques such as in situ FTIR (Fourier transform infrared) spectroscopy, GC–MS (gas chromatography–mass spectroscopy), and near-ambient pressure X-ray photoelectron spectroscopy were employed to verify gas interactions and their byproducts. Additionally, finite-difference time-domain simulations were used to model the electromagnetic field distribution and provide insight into the interaction between NO₂ molecules and the sensor surface at the nanoscale. A prototype wireless IoT (Internet of Things)-based NO₂ gas leakage detection system was also developed, showcasing the sensor’s practical application. This study offers valuable insight into the development of room-temperature NO₂ sensors with a low detection limit.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"21 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142763045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxygen Vacancy Mediated-Bismuth Molybdate/Graphitic Carbon Nitride Type II Heterojunction Chemiresistor for Efficient NH3 Detection at Room Temperature","authors":"Kaidi Wu, Xinzhu Qiu, Yifan Luo, Chao Zhang","doi":"10.1021/acssensors.4c02307","DOIUrl":"https://doi.org/10.1021/acssensors.4c02307","url":null,"abstract":"Metal oxide-based chemiresistive gas sensors are expected to play a significant role in assessing human health and evaluating food spoilage. However, the high operating temperature, insufficient limit of detection (LOD), and long response/recovery time restrict their broad application. Herein, 3D Bi₂MoO₆/2D Eg-C₃N₄ heterocomposites are developed for advanced NH₃ gas sensors with RT operational mode. Utilizing the synergetic engineering of micro-nanostructure, surface oxygen vacancies, and well-defined Type II n–n heterojunctions, BMOCN3 demonstrated superior NH₃ sensing properties at 23 °C, including the high response (S = 13.6 at 10 ppm), fast response/recovery speed (8/30 s), excellent selectivity, and low LOD (166 ppb). Based on the experimental, DFT, and MD studies, the improved sensing performance can be ascribed to accelerated charge transfer, superior redox capacity, and improved adsorption/desorption kinetics. Moreover, the practical application in rapid exhaled NH₃ biomarker detection of the as-fabricated gas sensor was preliminarily verified. This work highlights that the novel synergetic engineering can effectively modulate the electronic structure and charge transfer, offering a rational solution for room temperature chemiresistive gas sensors.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"19 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142763046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Field Deployment of a ppb-Level SO2/NO2 Dual-Gas Sensor System for Agricultural Early Fire Identification","authors":"Gangyun Guan, Qiang Wu, Anqi Liu, Mingquan Pi, Fang Song, Jie Zheng, Yiding Wang, Yu Zhang, Xue Bai, Chuantao Zheng","doi":"10.1021/acssensors.4c02405","DOIUrl":"https://doi.org/10.1021/acssensors.4c02405","url":null,"abstract":"Sulfur dioxide (SO₂) and nitrogen dioxide (NO₂) are chemical indicators of crop straw combustion as well as significant atmospheric pollutants. It is challenging to promptly detect natural “wildfires” during agricultural production, which often lead to uncontrollable and substantial economic losses. Moreover, both “wildfires” and artificial “straw burning” practices pose severe threats to the ecological environment and human health. Consequently, developing sensors capable of rapid and high-precision quantitative analysis of SO₂/NO₂ is essential and urgent for detecting early fires in agricultural activities. Here, we demonstrate an incoherent broadband cavity-enhanced absorption spectroscopy (IBBCEAS) sensing system utilizing a 366 nm ultraviolet light emitting diode, designed for real-time, high-precision monitoring of SO₂ and NO₂ and is used for early fire detection validation. The optical resonant cavity is constructed within a 60 mm cage system mechanical structure, achieving a maximum optical path length of nearly 2 km with a length of ∼460 mm. The output light carrying information about the species and concentration of the analyte molecules is coupled into the miniaturized grating spectrometer via a fiber, and continuous spectral fitting and concentration inversion are performed on the computer. We propose a spectral analysis and concentration inversion model based on an improved particle swarm optimization-support vector machine (IPSO-SVM) algorithm. By discrimination of the absorption spectral characteristics of SO₂/NO₂, we achieve superior prediction accuracy. Experimental results indicate that the detection limits of SO₂ and NO₂ under the optimized averaging time are 77.5 parts per billion by volume (ppbv) and 0.037 ppbv, respectively. The field deployment of the sensor in scenarios such as continuous outdoor air pollution monitoring, in situ combustion feature identification, and early fire mobile detection has demonstrated the superior reliability and sensitivity of this sensor system.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"116 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142760096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}