ACS Sensors最新文献_第5页

Engineered Antifouling Peptides with Sarcosine Branches for Robust Electrochemical Detection of the HER2 Biomarker in Real Biological Samples. 具有肌氨酸分支的工程防污肽用于真实生物样品中HER2生物标志物的鲁棒电化学检测。

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-05-25 DOI: 10.1021/acssensors.5c00082

Hao Zhou,Mingjun Shi,Wenhao Yang,Ning Song,Xiliang Luo

{"title":"Engineered Antifouling Peptides with Sarcosine Branches for Robust Electrochemical Detection of the HER2 Biomarker in Real Biological Samples.","authors":"Hao Zhou,Mingjun Shi,Wenhao Yang,Ning Song,Xiliang Luo","doi":"10.1021/acssensors.5c00082","DOIUrl":"https://doi.org/10.1021/acssensors.5c00082","url":null,"abstract":"In complex biological matrices, the nonspecific adsorption phenomena occurring on the surfaces of electrochemical biosensors represent a considerable challenge for the precise detection of targets in heterogeneous biological samples. Furthermore, the presence of protein hydrolases in biofluids also affects the stability of biosensing devices utilizing natural proteins or peptides. It is therefore imperative to develop sensing devices capable of effectively minimizing such effects in real biological samples. Herein, we engineered a sarcosine branch-chain peptide (SBCP) with a strong antifouling capability to avoid biofouling and enhanced stability to resist hydrolysis by proteases. The peptide is composed of three sections: an anchoring sequence (CPPPP), an antifouling sequence (EK(Sar)EK(Sar)EK(Sar)EK(Sar)), and a recognition sequence (HLTVSPWY). An electrochemical biosensor was developed through the electrodeposition of poly(3,4-ethylenedioxythiophene) (PEDOT) incorporated with poly(norepinephrine) (PNE) on an electrode surface, followed by the electrodeposition of gold nanoparticles and the self-assembly of SBCP. The biosensor constructed using the SBCP containing a specific recognizing peptide sequence for the cancer biomarker human epidermal growth factor receptor 2 (HER2) was capable of sensitively detecting target HER2, within the concentration range of 1.0 pg mL-1 to 1.0 μg mL-1 and with a limit of detection of 0.37 pg mL-1. Moreover, the biosensor demonstrated antifouling ability and the capacity to accurately detect the target in human serum, exhibiting a high degree of concordance with the assaying results of ELISA kits. These findings suggest that the biosensor based on the engineered peptides possesses promising potential for practical applications.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"33 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wearable Sensors: An ACS Sensors Special Issue 可穿戴传感器：ACS传感器专刊

IF 8.2 1区化学

ACS Sensors Pub Date : 2025-05-23 DOI: 10.1021/acssensors.5c0153010.1021/acssensors.5c01530

Yingying Zhang*,

引用次数: 0

Endoplasmic Reticulum Stress Induces Liquid-Liquid Phase Separation of GRP78 and Modulates Protein Aggregation Dynamics. 内质网应激诱导GRP78液液相分离并调节蛋白质聚集动力学。

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-05-23 DOI: 10.1021/acssensors.5c00807

Jiaqi Li,Xiangyu Zi,Jiabao Fang,Min Liang,Minzi Ju,Zhenglong Sun,Baoxing Shen,Xin Zhang

{"title":"Endoplasmic Reticulum Stress Induces Liquid-Liquid Phase Separation of GRP78 and Modulates Protein Aggregation Dynamics.","authors":"Jiaqi Li,Xiangyu Zi,Jiabao Fang,Min Liang,Minzi Ju,Zhenglong Sun,Baoxing Shen,Xin Zhang","doi":"10.1021/acssensors.5c00807","DOIUrl":"https://doi.org/10.1021/acssensors.5c00807","url":null,"abstract":"Abnormal protein aggregation is a hallmark of neurodegenerative diseases, disrupting cellular homeostasis. Glucose-regulated protein 78 (GRP78), a key endoplasmic reticulum (ER) chaperone, plays a crucial role in protein folding and the ER stress response. Recent studies suggest that GRP78 undergoes liquid-liquid phase separation (LLPS) to form dynamic condensates; however, its functional implications under pathological conditions remain unclear. In this study, we designed and synthesized two fluorescent probes (ER-Pro and Agg-Pro) for specifically labeling GRP78 and monitoring microenvironmental polarity changes during protein phase transition. By integrating fluorescence lifetime imaging microscopy and confocal microscopy, we demonstrated that GRP78 undergoes LLPS under ER stress and recruits the amyotrophic lateral sclerosis-associated mutant protein SOD1(A4V), influencing its aggregation dynamics. Further investigations revealed that SOD1(A4V) aggregation is accompanied by local polarity changes, highlighting a potential role for GRP78 LLPS in protein quality control. Our findings provide new insights into ER homeostasis regulation and the pathogenesis of neurodegenerative diseases, offering potential strategies for early diagnosis and therapeutic intervention.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"20 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144122390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wearable Sensors: An ACS Sensors Special Issue. 可穿戴传感器：ACS传感器专刊。

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-05-23 DOI: 10.1021/acssensors.5c01530

Yingying Zhang

引用次数: 0

Direct Current Redox Imaging of Single Retinal Pigment Epithelial Cells Using Light-Addressable Electrochemistry at Semiconductor Electrodes. 利用半导体电极的光寻址电化学技术对单个视网膜色素上皮细胞进行直流氧化还原成像。

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-05-22 DOI: 10.1021/acssensors.5c00587

Mingrui Jiang,Sen Wang,Kangying Liu,Yao Meng,Qiaoyu Yang,Jian Wang,De-Wen Zhang,Qiang Shi

{"title":"Direct Current Redox Imaging of Single Retinal Pigment Epithelial Cells Using Light-Addressable Electrochemistry at Semiconductor Electrodes.","authors":"Mingrui Jiang,Sen Wang,Kangying Liu,Yao Meng,Qiaoyu Yang,Jian Wang,De-Wen Zhang,Qiang Shi","doi":"10.1021/acssensors.5c00587","DOIUrl":"https://doi.org/10.1021/acssensors.5c00587","url":null,"abstract":"Light-addressable electrochemistry (LAE) enables the activation and detection of localized faradaic electrochemical processes on a flat, unconstructed semiconductor electrode through targeted light illumination, making it a promising approach for single-cell electrochemistry. Herein, we report a direct current (DC) redox imaging technique for single retinal pigment epithelial cells utilizing LAE with an α-Fe2O3 electrode activated by a constant, focused laser beam. The proposed DC-LAE method showed micron-scale resolution and was successfully used to image single cells under physiological conditions. We demonstrated that the visualization was primarily due to the hindrance of photoinduced OH- oxidation by adherent cells, resulting in a reduction of local photocurrents. Inspired by this funding, electroactive substances with high redox activities, such as hydroquinone (HQ), l-ascorbic acid (AA), and potassium ferricyanide (K4Fe(CN)6) were introduced into the culture medium. These substances significantly enhanced the DC redox imaging performance under low-intensity laser conditions without compromising cell viability. We believe that integrating pure DC-LAE with cell imaging advances quantitative analyses of cellular electrochemical behavior, offering valuable insights into cellular functions and processes.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"17 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144114307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterostructure of Chemical Vapor Deposition (CVD) Graphene and H2 Plasma-Treated SnO2 Nanoparticle Films for Room Temperature Sensing of SO2. 化学气相沉积（CVD）石墨烯和H2等离子体处理SnO2纳米颗粒薄膜的异质结构用于SO2的室温传感。

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-05-22 DOI: 10.1021/acssensors.5c00045

Yang Liu,Tianxing Chen,Yuming Chen

{"title":"Heterostructure of Chemical Vapor Deposition (CVD) Graphene and H2 Plasma-Treated SnO2 Nanoparticle Films for Room Temperature Sensing of SO2.","authors":"Yang Liu,Tianxing Chen,Yuming Chen","doi":"10.1021/acssensors.5c00045","DOIUrl":"https://doi.org/10.1021/acssensors.5c00045","url":null,"abstract":"In this article, we aim to develop SO2 sensors that operate at room temperature (25 °C) by using graphene synthesized through chemical vapor deposition and spin-coated SnO2 films. H2 plasma treatment was applied to the SnO2 films, and photolithography was used to pattern the graphene into interdigital electrodes. We found that the H2 plasma treatment induced oxygen vacancies, hydroxyl groups, and other types of surface states in the SnO2 films. The hydroxyl groups could be an important reason for the onset of p-type conduction in the SnO2 film. When graphene electrodes were deposited on top of the H2 plasma-treated SnO2 film, the SO2 sensor's response was enhanced by nearly 50 times (from 4% to 190%) compared to the sensor based solely on SnO2. Additionally, the response and recovery times were reduced by 34% and 91%, respectively. This performance remained stable even after the sensor had been stored in air for over 2 months. The substrate-mediated effects on the adsorption properties of the exposed graphene surface in the graphene-metal oxide heterostructure, along with the unique properties of the SnO2 film introduced by H2 plasma treatment, highlight new strategies for the future development of room-temperature-operated gas sensors.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"263 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144114308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amplification-Free CRISPR/Cas12a-Based Electrochemical Biosensor with Enhanced Sensitivity for Viral Detection. 无扩增的基于CRISPR/ cas12的电化学生物传感器，增强了病毒检测的灵敏度。

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-05-22 DOI: 10.1021/acssensors.5c00576

Yejin Lee,Jin-Ho Lee,Taek Lee,Minkyu Shin,Jinho Yoon

{"title":"Amplification-Free CRISPR/Cas12a-Based Electrochemical Biosensor with Enhanced Sensitivity for Viral Detection.","authors":"Yejin Lee,Jin-Ho Lee,Taek Lee,Minkyu Shin,Jinho Yoon","doi":"10.1021/acssensors.5c00576","DOIUrl":"https://doi.org/10.1021/acssensors.5c00576","url":null,"abstract":"To detect contagious viral nucleic acids, traditional biosensors often require target amplification steps or use fluorescence and Raman probes tagged on nucleic acids, which are time-consuming, complex, and expensive. Recently, the CRISPR/Cas12a has received the attraction for development of nucleic acid biosensors, beyond its conventional role-like gene editing, but the enhancement of the sensitivity of CRISPR/Cas-based biosensors is still required to simplify the biosensing steps. Here, we develop a CRISPR/Cas12a-based electrochemical biosensor for the detection of viral nucleic acids in a simple manner. The novel mismatch Ag probe (MAP), as a sensing probe, and the highly conductive gold electrode on indium tin oxide with a nano array (GELITION) are introduced that enable the amplification-free and ultrasensitive detection of nucleic acids using a CRISPR/Cas12a system. The biosensing ability of the developed biosensor is validated using human papillomavirus type 16 and 18 viral DNAs (HPV16 and HPV18), achieving a limit of detection (LOD) of 1 fM without amplification and complex steps. Our developed biosensor is expected to be applicable in detecting various viruses and could contribute to the early detection of future pandemics.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"5 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144122173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Standardization of Microsampling Technologies for Accurate Sensing and Reliable Diagnostics. 精确传感和可靠诊断的微采样技术标准化。

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-05-22 DOI: 10.1021/acssensors.5c00667

Andrea C Mora,Charles R Mace

{"title":"Standardization of Microsampling Technologies for Accurate Sensing and Reliable Diagnostics.","authors":"Andrea C Mora,Charles R Mace","doi":"10.1021/acssensors.5c00667","DOIUrl":"https://doi.org/10.1021/acssensors.5c00667","url":null,"abstract":"The COVID-19 pandemic demonstrated the debilitating effect of overreliance on centralized sampling and testing, and generated significant momentum to investigate and validate sample types that are alternatives to the gold standard (e.g., anterior nasal vs nasopharyngeal swabs). As a result, sample collection is trending away from traditional methods at centralized health care facilities and toward at-home or point-of-care settings with devices that enable self-collection. With shifts away from regulated environments and trained personnel, it is important to remember that reliable measurements begin with standardized sample collection. To develop microsampling technologies for applications where the outcome can impact health care decisions, it is critical to (i) fully understand the sample collected (e.g., volume, composition, biological factors) and (ii) implement engineering controls for users and clinical workflow integration (e.g., usability, volume metering) to ensure precision and accuracy of a measurement. Blood sampling, once exclusively performed via venipuncture, has made the greatest strides toward remote collection with the introduction of lancet- and microneedle-based, capillary microsampling devices. However, challenges remain toward standardizing these methods, such as ensuring that measurements are hematocrit-independent. Other sample types (e.g., nasal fluid, saliva) face similar hurdles. In this Perspective, we review the current state of self-collection microsampling technologies and highlight the need to develop tools that can (i) standardize sample collection and (ii) seamlessly integrate with current clinical workflows. Ultimately, we advocate for continued innovation as technologies that support self-collection have the potential to greatly improve the diagnostic process for patients, reduce the burden on health care workers, and advance the shift toward decentralized testing.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"38 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144114306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasensitive and Selective Detection of Dopamine Through Substituent-Regulated Evolution of Quantum Defects. 通过取代基调控的量子缺陷进化超灵敏和选择性检测多巴胺。

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-05-21 DOI: 10.1021/acssensors.5c01153

Taishan Yin,Yuqing Zhao,Jiaqi Zhang,Xian Xiao,Yue Huang,Bilian Ke,Zhongjie Huang

{"title":"Ultrasensitive and Selective Detection of Dopamine Through Substituent-Regulated Evolution of Quantum Defects.","authors":"Taishan Yin,Yuqing Zhao,Jiaqi Zhang,Xian Xiao,Yue Huang,Bilian Ke,Zhongjie Huang","doi":"10.1021/acssensors.5c01153","DOIUrl":"https://doi.org/10.1021/acssensors.5c01153","url":null,"abstract":"Accurate detection and analysis of biomolecules like dopamine (DA) are vital for monitoring human health, particularly given DA's critical roles in a lot of medical disorders such as depression, Parkinson's and Alzheimer's diseases, and myopia. DA is often found at very low concentrations within certain body fluids, making it a challenging yet essential target for detection. This study presents an innovative and ultrasensitive detection methodology based on a quantum system, characterized by its exceptional sensitivity, selectivity, and linearity. By leveraging the unique quantum defect emission from semiconducting single-walled carbon nanotubes (SWCNTs) in the near-infrared II region, our approach effectively detects DA with high sensitivity, within the physiologically relevant range of nanomolar, and a detection limit as low as 1 nM. The sensing system maintains performance in phosphate-buffered saline and human urine environments. The interaction between aryldiazonium salts and DA that generates sp3 defects on the SWCNTs surface, regulated by specific substituents on the benzene ring, dictates the sensor's performance, ensuring superior selectivity against biologically relevant molecules. These advancements hold great potential for early disease detection, prevention, and treatment, marking an important advance in the field of biomedical diagnostics and nanosensor research.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"18 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144103791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitrogen Plasma-Driven Oxygen Vacancy Modulation in Tin Dioxide Nanosheets Enables Sub-Parts-per-Billion Nitrogen Dioxide Detection at Low Operating Temperature. 二氧化锡纳米片中的氮等离子体驱动氧空位调制能够在低工作温度下检测到十亿分之一的二氧化氮。

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-05-21 DOI: 10.1021/acssensors.5c00298

Yaozong Zhai,Rui Yang,Jian Wu,Jingzhu Li,Kuimu Xiang,Nantao Hu,Min Zeng,Jianhua Yang,Zhi Yang

{"title":"Nitrogen Plasma-Driven Oxygen Vacancy Modulation in Tin Dioxide Nanosheets Enables Sub-Parts-per-Billion Nitrogen Dioxide Detection at Low Operating Temperature.","authors":"Yaozong Zhai,Rui Yang,Jian Wu,Jingzhu Li,Kuimu Xiang,Nantao Hu,Min Zeng,Jianhua Yang,Zhi Yang","doi":"10.1021/acssensors.5c00298","DOIUrl":"https://doi.org/10.1021/acssensors.5c00298","url":null,"abstract":"Metal oxide semiconductor (MOS) materials have been widely used in gas sensing. However, they generally face challenges such as high operating temperatures and limited sensitivity/selectivity, which hinder their applications in areas like medical diagnosis based on human exhaled breath and ultralow concentration gas detection in harsh environments. Developing general strategies to enhance the sensing performance of MOS materials is both challenging and highly desired. Herein, we demonstrate nitrogen plasma-driven oxygen vacancy modulation in tin dioxide nanosheets (SnO2 NSs) that enables sub-parts-per-billion-level nitrogen dioxide (NO2) detection at low temperatures. SnO2 NSs, oriented predominantly along the (110) crystal facet, are synthesized using graphene oxide templates and treated with nitrogen plasma, which can generate abundant oxygen vacancies. The oxygen vacancy-rich SnO2 NSs exhibit exceptional NO2 sensing performance, with a theoretical detection limit of 0.154 ppb and a response that is 3.4 times higher than that of the untreated SnO2 NSs at 80 °C. Mechanism studies reveal that the improved sensitivity is attributed to the large surface area, favorable crystal orientation, and oxygen vacancies introduced by nitrogen plasma treatment. This work not only provides a promising strategy for modulating the oxygen vacancies in MOS materials, but also offers valuable insights for the development of high-performance MOS-based gas sensors.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"27 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144103792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0