Elsevier最新文献

{"title":"Metal-organic framework-enhanced Electrochemiluminescence of pyrene-based ligands for sensitive CEA detection.","authors":"Tiantian Ji, Yige Li, Mingzhe Jiang, Yingying Cheng, Haoyi Ren, Hongling Li, Chenglin Hong","doi":"10.1016/j.bioelechem.2025.109073","DOIUrl":"10.1016/j.bioelechem.2025.109073","url":null,"abstract":"<p><p>The early and sensitive detection of cancer, a malignant disease posing significant threats to human health, is of strategic importance for disease prevention and control. This study employed 1,3,6,8-tetra(4-carboxyphenyl)pyrene (H₄TBAPy), a fluorophore exhibiting aggregation-caused quenching (ACQ), to construct a zinc-based metal-organic framework (Zn-TBAPy) serving as an energy donor platform. Polydopamine-coated ZIF-67 (ZIF-67@PDA) was employed as the energy acceptor to construct an electrochemiluminescence (ECL) immunosensor for sensitive carcinoembryonic antigen (CEA) detection. The advantages of ECL immunosensor are primarily manifested in the following three aspects: (1) Zn-TBAPy not only mitigates ACQ caused by polycyclic aromatic hydrocarbon π-π stacking but also enhances chromophore loading capacity and specific surface area. Relative to aggregate systems, the Zn-TBAPy exhibits a 2.5-fold enhancement in ECL signal intensity. (2) ZIF-67@PDA exhibits favorable broad-spectrum absorption characteristics and excellent quenching efficiency; as well as demonstrates superior biocompatibility for immunosensor construction. (3) The immunosensor was constructed through an electrochemiluminescence resonance energy transfer (ECL-RET) mechanism, yielding markedly improved sensitivity; the developed sensor demonstrated a linear detection range from100 fg·mL^-1 to 80 ng·mL^-1with LOD) of 0.275 pg·mL^-1. In conclusion, this study provides a valuable research strategy for the construction of immunosensors based on novel luminophore materials.</p>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"167 ","pages":"109073"},"PeriodicalIF":4.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marine-tolerant bioadhesive gel with hydrophobic microdomain-multicrosslinked network for seawater-immersed wound management.","authors":"Mingyu Yu, Xianglong Zhong, Wanting Zhang, Wenwen Zhang, Zhiming Liu, Jia Song, Dantong Wang, Ruixin Wang, Chuandong He, Yanlv Chen, Yitong Zhou, Kangrui Yuan, Jiayuan Lin, Yuhan Jiang, Xiyu Cai, Xin Peng","doi":"10.1016/j.biomaterials.2025.123601","DOIUrl":"10.1016/j.biomaterials.2025.123601","url":null,"abstract":"<p><p>Current gel dressings face significant challenges in seawater-immersed wound management due to their marine-intolerance, poor bioadhesion and non-antibacterial properties. Herein, we develop a multifunctional gel that integrates marine-tolerance, wet adhesion, non-invasive detachment, good antibacterial properties to resist bleeding and promote wound healing in marine environments. Our design strategy employs solvent-exchange-induced self-assembly of hydrophobic segments to engineer hydrophobic microdomains, coupled with the synergistic effects of hydrogen/ionic/coordination bonds as multicrosslinked networks, resulting in a marine-tolerant hydrogel with a \"hydrophobic microdomain-multicrosslinked\" network structure. An \"interfacial drainage-multivalent bonding\" dual-effect adhesion strategy is proposed: the interfacial drainage effect induced by silicone oil and hydrophobic microdomains enables tight tissue-gel anchoring, while the cooperative interactions of hydrogen/carbon-nitrogen/carbon-sulfur bonds synergistically achieve strong interfacial adhesion, achieving stable wet adhesion in marine environments. Furthermore, glutathione can cleave the disulfide bonds within the gel and the carbon-sulfur bonds between the gel and tissue, facilitating non-invasive detachment. Besides, the incorporation of zinc oxide nanoparticles provides broad-spectrum antibacterial functionality. Comparative animal experiments demonstrate superior performance over commercial glue in hemostatic efficiency and wound regeneration under marine conditions. This multifunctional hydrogel system establishes a new paradigm for developing advanced marine medical biomaterials through the rational integration of structural engineering and functional components.</p>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"325 ","pages":"123601"},"PeriodicalIF":12.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cancer-associated fibroblasts regulating nanomedicine to overcome sorafenib resistance in hepatocellular carcinoma with portal vein tumor thrombus.","authors":"Lin Zhu, Mali Zu, Feifan Wu, Xu Ma, Shuhui Zhang, Tianchen Zhang, Xingfan Li, Huiru Yang, Zhenqi Li, Zetao Yu, Ruijie Nie, Tianjiao Ji, Xiuping Zhang, Rong Liu","doi":"10.1016/j.biomaterials.2025.123599","DOIUrl":"10.1016/j.biomaterials.2025.123599","url":null,"abstract":"<p><p>Portal vein tumor thrombus (PVTT) is a common and severe indicator in advanced hepatocellular carcinoma (HCC), characterized by a poor prognosis and limited response to existing therapies. Cancer-associated fibroblasts (CAFs) play an important role in promoting HCC metastasis and contribute to resistance against sorafenib (SOR) resistance, which is a standard treatment for advanced HCC. The data from single-cell RNA sequencing highlights the critical role of C-X-C motif chemokine ligand 12 (CXCL12) in the activation of CAFs. To address these challenges, we develop a PVTT-targeted nanocarrier designed to co-deliver small interfering RNA (siRNA) and a multikinase inhibitor, aiming to enhance therapeutic outcomes for PVTT. This novel lipid-coated polylactide-co-glycolide nanoparticle system effectively downregulate CXCL12 expression in CAFs, leading to their inactivation and subsequent reshaping of the tumor microenvironment. The resulting modulation of the tumor microenvironment significantly suppress tumor cell migration, invasion, and resistance to SOR, thereby demonstrating potent anti-tumor effects in orthotopic mouse models of PVTT. Furthermore, RNA sequencing reveals key regulatory pathways and genes associated with the inhibition of SOR resistance and PVTT formation mediated by these nanoparticles. These findings suggest that modulating the tumor microenvironment, combined with targeted anti-tumor therapies, offers a promising strategy for treating HCC patients with PVTT.</p>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"325 ","pages":"123599"},"PeriodicalIF":12.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced osteointegration of implants in aged rats via a stem cell pool aging reversion strategy.","authors":"Xuan Li, Xinxin Luo, Ye He, Bikun Zhou, Kun Xu, Qian Huang, Xiao Jiang, Hongwei Xiong, Xuezhe Liu, Shaopeng Liu, Bailong Tao, Peng Liu, Kaiyong Cai","doi":"10.1016/j.biomaterials.2025.123604","DOIUrl":"10.1016/j.biomaterials.2025.123604","url":null,"abstract":"<p><p>The senescence of mesenchymal stem cells (MSCs) leads to the significant change of their metabolic activity and physiological behaviors. In the context of orthopedic treatment, the osteointegration of titanium implant is largely affected by MSC aging, imposing considerable limitations on its long-term application. In this study, a surface modification on titanium implants was designed to enhance osteointegration by effectively regulating the functions of senescent MSC: A typical micro-nano topological structure was established on the implant surface to improve the osteogenic differentiation of MSCs. Then a functional hydrogel coating was covalently modified to the implant surface through a poly-dopamine layer. For senescent MSCs, firstly, the coating can eliminate the activation of senescence-associated secretory phenotype (SASP) of senescent MSCs by micro-nano topological structure, and it accelerated the proliferation of non-senescent MSCs by the reactive oxygen species (ROS) scavenging. With the degradation of the hydrogel coating, the composition of stem cell pool around the implant interfaces gradually rejuvenated, as the number of non-senescent MSCs increased and senescent MSCs decreased. Meanwhile, the exposed micro-nano topological structure showed significant effect on the osteogenic differentiation of MSCs, and ultimately promoted the osteointegration in aging rats. These results provided promising insights for the design and application of orthopedic titanium implants for aging patients.</p>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"325 ","pages":"123604"},"PeriodicalIF":12.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A sprayable TQ/Ce6@SAB/F-gel for accelerating wound healing via hypoxia-tolerant photodynamic therapy and immune-metabolic pathway.","authors":"Ming Li, Shengzhe Zhou, Qiang Yu, Chenxi Wang, Haoyi Chen, Yingying Ma, Huizhen Fan, Tao Ni, Min Lu, Min Yao","doi":"10.1016/j.biomaterials.2025.123602","DOIUrl":"10.1016/j.biomaterials.2025.123602","url":null,"abstract":"<p><p>Chronic diabetic wounds are characterized by hypoxia, persistent microbial infection, and impaired healing, posing significant challenges to conventional therapies. Herein, we present a novel sprayable double-network hydrogel platform designed to achieve efficient antimicrobial activity and accelerated wound repair under hypoxic conditions by leveraging a type I photodynamic therapy (PDT) and immune-metabolic regulatory strategy. Specifically, we employ salvianolic acid B (SAB) to form a self-assembled hydrogel (SAB-gel) and incorporate fibrin to construct a robust and acidic double-network SAB/F-gel with enhanced mechanical strength and acidic environment. Concurrently, thymoquinone (TQ) and chlorin e6 (Ce6) are self-assembled via hydrophobic interactions to form TQ/Ce6 nanoparticles (TQ/Ce6 NPs) and embedded in the SAB/F-gel, to fabricate the TQ/Ce6@SAB/F-gel. Under low-oxygen conditions, TQ acts as an electron-transfer mediator, enabling Ce6 to generate abundant superoxide anions (·O₂^-) via type I PDT under red light (RL) irradiation. These ·O₂^- are subsequently converted into hydrogen peroxide (H₂O₂) and hydroxyl radicals (·OH) in the acidic environment provided by acidic SAB/F-gel, thereby reducing the dependence on oxygen and maintaining potent antimicrobial efficacy against MRSA, Pseudomonas aeruginosa (Pa), Acinetobacter baumannii (Ab), Escherichia coli (E. coli) and Candida albicans (Ca). In vitro experiments demonstrated that TQ/Ce6@SAB/F-gel regulates macrophage M2 polarization and promotes endothelial cell proliferation, migration, and tube formation via the immune-metabolic regulatory pathways. When applied to MRSA-infected diabetic wounds in mice, the hydrogel in combination with RL completely eradicated bacteria, promoted collagen deposition and angiogenesis, and significantly accelerated wound closure, as demonstrated by histological examination and transcriptome sequencing. This work offers a versatile, biocompatible, and oxygen-independent PDT-based hydrogel system for the treatment of refractory infected diabetic wounds, offering potential for clinical translation and improved patient outcomes.</p>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"325 ","pages":"123602"},"PeriodicalIF":12.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-supported DNA hydrogel facilitates microenvironment remodeling and cartilage repair to prevent osteoarthritis progression via an ambidextrous strategy.","authors":"Yixin Liu, Xinjian Yang, Ya Miao, Taoping Chen, Wenyan Gao, Guoqiang Zhou, Guang Jia, Xiaosong Yang, Jinchao Zhang, Yi Jin","doi":"10.1016/j.biomaterials.2025.123595","DOIUrl":"10.1016/j.biomaterials.2025.123595","url":null,"abstract":"<p><p>Osteoarthritis (OA), a prevalent degenerative joint disease, currently lacks effective therapeutic options beyond symptomatic relief. Persistent inflammation and impaired cartilage repair accelerate the disease progression. The enzyme inducible nitric oxide synthase (iNOS) contributes to OA by producing nitric oxide (NO), which intensifies inflammation and inhibits cartilage regeneration. Traditional iNOS inhibitors have demonstrated limited efficacy due to inadequate targeted release and uncoordinated control over inflammation. In this study, we developed a self-supported DNAzyme-based DNA hydrogel using rolling circle amplification (RCA) technology to deliver iNOS-targeting DNAzymes and bone marrow mesenchymal stem cell-derived exosomes (BMSC-exos) in response to inflammation. The hydrogel incorporates triglycerol monostearate nanoparticles (TGMS NPs), which degrade under high matrix metalloproteinase (MMP) levels in OA joints, thereby triggering the release of the DNAzymes and exosomes with precision. This targeted delivery modulates the inflammatory microenvironment by reducing pro-inflammatory NO production and supports chondrogenesis by promoting M2 macrophage polarization. In vitro and in vivo analyses reveal that the hydrogel significantly reduces inflammatory cytokine levels, enhances chondrocyte proliferation, and restores extracellular matrix integrity, ultimately slowing OA progression. This smart hydrogel offers a promising ambidextrous strategy for microenvironment modulation and cartilage regeneration, potentially advancing OA treatment.</p>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"325 ","pages":"123595"},"PeriodicalIF":12.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functionalized magnetic nanoprobe-based aptasensor for on-site detection of kanamycin in water by a personal glucose meter.","authors":"Ting Lei, Chengyan Yin, Hui Feng, Xiangwen Lei, Ruth Antwi-Baah, Jing Liu, Li Zhang, Wulin Yang, Shuxia Xu","doi":"10.1016/j.talanta.2025.128515","DOIUrl":"10.1016/j.talanta.2025.128515","url":null,"abstract":"<p><p>In this work, the nanomagnetic probe was prepared by condensation of carboxylated kanamycin (Kana) aptamers with aminated nanomagnetic Fe₃O₄ beads, which was coupled with invertase-labeled complementary DNA (cDNA) for sensitive and convenient detection of Kana in water samples by a personal glucose meter (PGM). In the absence of Kana, there was no PGM signal recorded because formed nanomagnetic probe was removed by magnetic separation. Conversely, the aptamer could recognize Kana specifically and released cDNA into supernatant. After the addition of sucrose, it was hydrolyzed into glucose by invertase. Detection of Kana was indirectly achieved based on the relationship between glucose concentration and the PGM indication. Furthermore, a miniaturized portable device was constructed by integrating the components using 3D printing technology. Under optimal conditions, rapid detection of Kana within the concentration range of 1-200 nM was achieved, with a detection limit (3σ/k) of 0.28 nM. Moreover, the proposed aptasensor was characterized in terms of selectivity, reproducibility and stability. Finally, it was applied to the determination of Kana in water with recoveries from 99% to 103%. All these results indicated that the proposed aptasensor is simple, rapid, selective, and sensitive, and it could potentially serve as an effective approach for the on-site determination of Kana in water samples.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"296 ","pages":"128515"},"PeriodicalIF":6.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic and prognostic utility of endocan in suspected myocardial infarction: an international cohort study.","authors":"Franja Dugar, Pedro Lopez-Ayala, Luca Koechlin, Paolo Bima, Jonas Glaeser, Carlos Spagnuolo, Luca Crisanti, Clara Wick, Karin Wildi, Emel Kaplan, Ivo Strebel, Oscar Miro, Francisco Javier Martin-Sanchez, Michael Christ, Beata Morawiec, Maria Rubini Gimenez, Thomas Nestelberger, Jasper Boeddinghaus, Christian Mueller","doi":"10.1016/j.cca.2025.120510","DOIUrl":"10.1016/j.cca.2025.120510","url":null,"abstract":"<p><strong>Background: </strong>The possible clinical utility of endocan, a novel inflammatory biomarker involved in the initiation and progression of atherosclerosis, is largely unknown. We aimed to evaluate its diagnostic and prognostic performance in chest pain patients presenting to the emergency department (ED).</p><p><strong>Methods: </strong>We prospectively enrolled patients presenting with suspected myocardial infarction (MI) to the ED in an international multicenter study. Endocan, high-sensitivity C-reactive protein (hs-CRP), and high-sensitivity cardiac troponin T (hs-cTnT) were measured in blood samples obtained at presentation. Final diagnoses were centrally adjudicated by two independent cardiologists applying the 4th universal definition of MI and current guidelines. Non-ST-elevation MI (NSTEMI) was the diagnostic endpoint and 5-year cardiovascular death was the primary prognostic endpoint.</p><p><strong>Results: </strong>Among 4728 patients, 843 (17.8 %) had NSTEMI. The diagnostic discrimination of endocan for NSTEMI was low (area under the curve (AUC) 0.585 [95 % CI: 0.563-0.607]. Its combination with hs-cTnT (0.939 [95 % CI: 0.931-0.947]) did not improve the discriminative performance of hs-cTnT alone (0.937 [95 % CI: 0.930-0.950]). Long-term prognostic accuracy of endocan was higher versus hs-CRP, but lower versus hs-cTnT (AUC 0.730 [0.710-0.760] vs 0.650 [0.620-0.680] vs 0.810 [0.790-0.830], respectively). Endocan was associated with an increased 5-year risk for cardiovascular mortality. However, it did not provide relevant incremental prognostic value when added on top of a base model that included SCORE2 risk factors and hs-cTnT.</p><p><strong>Conclusion: </strong>Endocan has a low diagnostic accuracy for NSTEMI, and moderate long-term prognostic accuracy for cardiovascular death.</p><p><strong>Clinical trial registration: </strong>ClinicalTrials.gov number, NCT00470587, https://clinicaltrials.gov/ct2/show/NCT00470587https://clinicaltrials.gov/ct2/show/NCT00470587.</p>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":" ","pages":"120510"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144728330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aptamer biosensors in early breast cancer detection.","authors":"Hamdi Nsairat, Omer Qutaiba B Allela, Abdulkareem Shareef, S Renuka Jyothi, Priya Priyadarshini Nayak, Ashish Singh Chauhan, Hayder Naji Sameer, Ahmed Yaseen, Zainab H Athab, Mohaned Adil","doi":"10.1016/j.cca.2025.120536","DOIUrl":"10.1016/j.cca.2025.120536","url":null,"abstract":"<p><p>Breast cancer, a leading cause of cancer-related mortality among women, necessitates the development of sensitive and specific diagnostic tools for early detection and personalized treatment. An aptamer-based biosensor (aptasensor) is a biosensor that utilizes aptamers as its biorecognition element such as single-stranded DNA or RNA molecules that are specifically selected to bind to a target molecule with high affinity. Aptasensors have emerged as promising alternatives to traditional methods, offering advantages such as high affinity, specificity, and ease of synthesis. This review provides an overview of recent advancements in aptasensor technology for breast cancer diagnostics, focusing on the detection of key biomarkers. In this review, first after an overview to different types of aptasensors, we discuss the different type of aptamer immobilization methods on sensor surfaces using covalent, adsorption, and thiol-based self-assembly techniques. Furthermore, we present different aptasensor platforms, including electrochemical and optical approaches, highlighting their design principles, performance characteristics, and clinical applications.</p>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":" ","pages":"120536"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144798323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic approaches for biomarker discovery and clinical applications in autoimmune diseases.","authors":"Sultan Ayesh Mohammed Saghir, Amir M Al Hroob, Salah A Alshehade, Sulaiman Alnaimat, Nuha A Al Yousfi, Sarah Ahmad Bahjat Al-Rawashdeh, Mohammad Ahmad Al Rawashdeh, Mohammed Abdullah Alshawsh","doi":"10.1016/j.cca.2025.120533","DOIUrl":"10.1016/j.cca.2025.120533","url":null,"abstract":"<p><p>Autoimmune diseases (ADs) impact approximately 3% of the global population. It encompasses more than 80 chronic, often debilitating conditions resulting from immune system defects that lead the body to attack its tissues. Although many ADs are rare, their prevalence is rising. Despite advancements, ADs diagnosis and classification still rely heavily on clinical examination combined with conventional laboratory testing and imaging. Emerging proteomic screening, technologies now offer the potential to identify unique biomarkers for more precise diagnosis, classification, and treatment decisions. Protein profiling, an advancing area within proteomics, provides unparalleled insights into biological processes underlying these diseases. Thus, this review highlights recent developments in proteomic and genetic profiling for ADs pathogenesis, diagnosis, and treatment challenges, focusing on the clinical utility of proteomic techniques in prognosis, diagnosis, and therapeutic guidance. Notably, discovering new biomarkers through proteomic screening is crucial for creating robust multi-parameter assays, which enhance diagnostic accuracy and treatment decisions in ADs.</p>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":" ","pages":"120533"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}