Liyu Qiu, Xixiang Yang, Nan Zhao, Anqi Luo, Li Wang, Xiaogang Hu
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引用次数: 0
Abstract
Hybrids of molecular imprinting-aptamers have been used for environmental analysis; however, the weak selectivity and stability inhibited their applications in complex matrices. In this paper, the nano-scale pH-responsive aptamer-molecularly imprinted interpenetrating hydrogels (Fe3O4@Apt-MIH) were prepared in one step by the modified sol-gel method based on the preparation principle of interpenetrating network hydrogels, with Fe3O4 as the carrier, aminopropyltriethoxysilane and methyltrimethoxysilane as functional monomers, polyethyleneimine (PEI) as the pH-responsive components, and tetraethoxysilane (TEOS) and glutaraldehyde (GA) as dual cross-linking agents. After optimizing the polymerization and extraction conditions, the pH response performance, extraction capacity and reusability of the material with highly magnetism were investigated, which could achieve magnetic separation within 5 s. The SiO2 coating hydrolyzed by TEOS provided a rigid backbone for interpenetrating hydrogels, which could improve the acid resistance of PEI by adjusting the isoelectric point of Fe3O4@Apt-MIH and assist in target identification. Selectivity research showed that aptamers and functional monomers have a highly effective synergistic recognition effect on ampicillin, with the imprinting factor of 6.90 and the synergistic recognition factor of 1.48. This solid-phase microextraction material coupled to high-performance liquid chromatography with the linear range of 10-1000 μg/L and the detection limit of 7.67 μg/L enabled the selective separation and detection of ampicillin in milk and honey, with minimal sample matrix interference and recovery between 95.2 and 102 %.
期刊介绍：
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.
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