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Transforming agri-food waste into value: Sustainable approaches for next-generation resistant starch production. 将农业食品垃圾转化为价值:下一代抗性淀粉生产的可持续方法。
IF 9 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-12-01 Epub Date: 2025-08-06 DOI: 10.1016/j.biortech.2025.133090
Shikha Pandhi, Dipendra Kumar Mahato, Anju Kurian, Surabhi Pandey, Abhishek Thamarai, Vishwas Gaur, Madhu Kamle, Amritesh Chandra Shukla, Pradeep Kumar
{"title":"Transforming agri-food waste into value: Sustainable approaches for next-generation resistant starch production.","authors":"Shikha Pandhi, Dipendra Kumar Mahato, Anju Kurian, Surabhi Pandey, Abhishek Thamarai, Vishwas Gaur, Madhu Kamle, Amritesh Chandra Shukla, Pradeep Kumar","doi":"10.1016/j.biortech.2025.133090","DOIUrl":"10.1016/j.biortech.2025.133090","url":null,"abstract":"<p><p>Agri-food waste, which includes organic materials discarded throughout the food supply chain from agricultural production and post-harvest handling to processing, distribution, and consumption, poses significant environmental and economic challenges due to resource underutilization and disposal issues. However, the valorization of these residues into value-added products, particularly resistant starch (RS), offers a sustainable solution. RS, a non-digestible carbohydrate with prebiotic properties, provides numerous health benefits, including improved gut health, blood sugar regulation, and enhanced satiety. Various studies have explored innovative methods for RS extraction from agricultural by-products such as potato peels, rice bran, banana peels, and chestnut starch. Techniques such as enzymatic hydrolysis, ultrasound-assisted extraction, and thermal processing have demonstrated high efficiency in producing RS while maintaining its functional properties. Additionally, advancements in artificial intelligence (AI), big data, and machine learning are playing a crucial role in optimizing waste valorization processes. AI-driven technologies enable precise classification and separation of food waste, improving the efficiency of extraction and processing techniques. The integration of smart systems, such as IoT-based waste classification, further enhances resource recovery. As research and technological advancements continue, the large-scale application of these sustainable extraction and processing methods is expected to support a circular economy, reduce food waste, and provide functional food ingredients for the food industry.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133090"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Predicting biomass gasification products for bubbling fluidised beds using high order polynomial regression with regularisation: a simple but highly effective strategy. 用正则化的高阶多项式回归预测鼓泡流化床生物质气化产物:一种简单但高效的策略。
IF 9 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-12-01 Epub Date: 2025-08-06 DOI: 10.1016/j.biortech.2025.133109
Michael Binns
{"title":"Predicting biomass gasification products for bubbling fluidised beds using high order polynomial regression with regularisation: a simple but highly effective strategy.","authors":"Michael Binns","doi":"10.1016/j.biortech.2025.133109","DOIUrl":"10.1016/j.biortech.2025.133109","url":null,"abstract":"<p><p>For the design of biomass gasification it is beneficial to have models which can predict the composition of gas products for a wide range of different biomass feedstocks. Complex machine learning models (e.g. neural networks and tree-based methods) are now being used for this purpose which are difficult to reproduce with large numbers of parameters involved. In this study the potential for higher order polynomials is investigated for the modelling of bubbling fluidised bed gasification. To reduce the number of parameters and to avoid over-fitting Least Absolute Shrinkage and Selection Operator (LASSO) regularisation is used. This is a novel application of high order polynomial regression with regularisation which allows the prediction of hydrogen composition with coefficient of performance of 0.9228 and only 85 fitted parameters. The next best existing methods give coefficients of performance of 0.8823 and 0.868 but require 261 parameters and more than 1000 parameters respectively. So this polynomial approach is shown to give accurate model prediction with simpler model equations.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133109"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A commentary on "The relationship between unhealthy lifestyle behaviors and depression: Evidence from NHANES". 对“不健康的生活方式行为与抑郁症之间的关系:来自NHANES的证据”的评论。
IF 4.9 2区 医学
Journal of affective disorders Pub Date : 2025-12-01 Epub Date: 2025-07-14 DOI: 10.1016/j.jad.2025.119903
JingYing Ma, FaDan Tang, Jue Hu
{"title":"A commentary on \"The relationship between unhealthy lifestyle behaviors and depression: Evidence from NHANES\".","authors":"JingYing Ma, FaDan Tang, Jue Hu","doi":"10.1016/j.jad.2025.119903","DOIUrl":"10.1016/j.jad.2025.119903","url":null,"abstract":"","PeriodicalId":14963,"journal":{"name":"Journal of affective disorders","volume":" ","pages":"119903"},"PeriodicalIF":4.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A question of perspective: Target- vs. perceiver-specific dimensions of mind perception. 视角问题:心理感知的目标与感知者特定维度。
IF 2.8 1区 心理学
Cognition Pub Date : 2025-12-01 Epub Date: 2025-08-08 DOI: 10.1016/j.cognition.2025.106231
Nele J Bögemann, Lasana T Harris, Steffen Nestler
{"title":"A question of perspective: Target- vs. perceiver-specific dimensions of mind perception.","authors":"Nele J Bögemann, Lasana T Harris, Steffen Nestler","doi":"10.1016/j.cognition.2025.106231","DOIUrl":"10.1016/j.cognition.2025.106231","url":null,"abstract":"<p><p>Mind perception - the inference of mind in others - is foundational for social cognition and interaction, but previous research on its underlying dimensions has so far only produced mixed findings. In a prominent study, H.M. Gray et al. (2007) identified two dimensions of mind perception - Agency and Experience -, while more recent work instead suggests three dimensions similar to Body, Heart, and Mind (Malle, 2019; Weisman et al., 2017). Here, we provide a comprehensive account that can accommodate both dimensional structures by distinguishing target- from perceiver-specific dimensions of mind perception. These dimensions explain target- and perceiver-specific differences in mind perception that were differentially focused on by previous studies ascribing to the competing dimensional structures. To test our account empirically and compare target- vs. perceiver-specific dimensions, we gathered online survey data from two samples (N = 157, and N = 150). In both samples, exploratory factor analyses yielded two target-specific dimensions in line with Agency-Experience, and three perceiver-specific dimensions in line with Body-Heart-Mind, thereby validating our explanatory account. Further analyses showed that perceiver-specific dimensions are meaningfully associated with perceivers' demographics, personality, and spiritual belief; and that they depend on target context. Together, our results resolve inconsistencies in mind perception research and work toward a novel unifying mind perception framework.</p>","PeriodicalId":48455,"journal":{"name":"Cognition","volume":"265 ","pages":"106231"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144812593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Letter to the editor: Association between overactive bladder and depression in American adults: A cross-sectional study from NHANES 2005-2018. 致编辑的信:美国成年人膀胱过度活动与抑郁症之间的关系:来自NHANES 2005-2018的横断面研究。
IF 4.9 2区 医学
Journal of affective disorders Pub Date : 2025-12-01 Epub Date: 2025-08-13 DOI: 10.1016/j.jad.2025.120055
Linkun Shen, Sheng Li
{"title":"Letter to the editor: Association between overactive bladder and depression in American adults: A cross-sectional study from NHANES 2005-2018.","authors":"Linkun Shen, Sheng Li","doi":"10.1016/j.jad.2025.120055","DOIUrl":"10.1016/j.jad.2025.120055","url":null,"abstract":"","PeriodicalId":14963,"journal":{"name":"Journal of affective disorders","volume":" ","pages":"120055"},"PeriodicalIF":4.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144859207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Coupling mechanisms of community assembly and pollutant removal in algal-bacterial granular sludge systems. 藻-细菌颗粒污泥系统中群落聚集与污染物去除的耦合机制。
IF 9 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-12-01 Epub Date: 2025-08-06 DOI: 10.1016/j.biortech.2025.133122
Rushuo Yang, Zhe Liu, Yongjun Liu, Zhuangzhuang Yang, Zhu Wang, Yuhang Zhang, Jie Lei, Tianyu Han, Jiaxuan Wang, Zhihua Li
{"title":"Coupling mechanisms of community assembly and pollutant removal in algal-bacterial granular sludge systems.","authors":"Rushuo Yang, Zhe Liu, Yongjun Liu, Zhuangzhuang Yang, Zhu Wang, Yuhang Zhang, Jie Lei, Tianyu Han, Jiaxuan Wang, Zhihua Li","doi":"10.1016/j.biortech.2025.133122","DOIUrl":"10.1016/j.biortech.2025.133122","url":null,"abstract":"<p><p>This study comparatively assessed algal-bacterial granular sludge (ABGS) decontamination performance and microbial community mechanisms under two cultivation modes: flocculated sludge transformed into ABGS (AS_ABGS) and aerobic granular sludge transformed into ABGS (AGS_ABGS). The results indicated that, AS_ABGS achieved superior pollutant removal (COD: 92.2 %, TN: 82.1 %, TP: 61.5 %) versus AGS_ABGS (COD: 96.3 %, TN: 75.1 %, TP: 53.3 %). Microbial community analysis revealed deterministic assembly dominated AS_ABGS, narrowing niches, and enhancing functional specialization of N/P-removing taxa. AS_ABGS exhibited higher modularity, robustness, and stronger positive algal-bacterial interactions (52.32 %). Conversely, stochastic assembly in AGS_ABGS yielded weaker interactions (50.45 %). Metagenomics confirmed AS_ABGS enriched N/P metabolic genes (amo, acc) may be driven by Thauera, Micavibrio, and Aquisediminimonas, while AGS_ABGS favored Amaricoccus and Rhodovulum but showed lower N/P gene abundance. This study highlights the effect of algal-bacterial ecological interactions mediated by functional genes on the decontamination efficiency of ABGS and provides valuable insights for advancing this method.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133122"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhancing biochar quality for the steel industry via Hydrothermal Pretreatment-Steam Explosion and pyrolysis. 通过水热预处理-蒸汽爆炸和热解提高钢铁工业生物炭的质量。
IF 9 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-12-01 Epub Date: 2025-08-07 DOI: 10.1016/j.biortech.2025.133009
Chay A Davies-Smith, Julian Herbert, Ciarán Martin, Darbaz Khasraw, David Warren-Walker, David Bryant, Joe Gallagher, Gordon Allison, Julian M Steer, Richard Marsh, Ahmed Alsawadi, Rakesh Bhatia
{"title":"Enhancing biochar quality for the steel industry via Hydrothermal Pretreatment-Steam Explosion and pyrolysis.","authors":"Chay A Davies-Smith, Julian Herbert, Ciarán Martin, Darbaz Khasraw, David Warren-Walker, David Bryant, Joe Gallagher, Gordon Allison, Julian M Steer, Richard Marsh, Ahmed Alsawadi, Rakesh Bhatia","doi":"10.1016/j.biortech.2025.133009","DOIUrl":"10.1016/j.biortech.2025.133009","url":null,"abstract":"<p><p>Biochar has potential applications in steelmaking processes, but faces technical challenges such as low material density, high alkali content, and high reactivity compared to coal. This study explores converting the solid residue, following hydrothermal pretreatment-steam explosion (HTP-SE) of Miscanthus and other biomass feedstocks, into biochar to facilitate the replacement of coal in blast furnace and electric arc furnace operations. It is the first to demonstrate the enhanced combustion characteristics of pretreated fibre and the compatibility of the biochar for use in steelmaking. Biomass from birch, miscanthus, wheat straw, both untreated and pretreated, was evaluated. HTP-SE was conducted at 192 °C and 1.3 MPa, conditions aligned with hemicellulose extraction for application in biobased products. Biochars were produced at temperatures ranging from 300 °C to 550 °C. HTP-SE increased the carbon, hydrogen, and energy content by approximately 10%, 8%, and up to 5 MJ/kg, respectively, while reducing ash quantity by up to 45%. In addition, it reduced the alkali and phosphorus content from the solid fraction into aqueous phase. Gas analysis indicated that HTP-SE enhanced the energy content of pyrolysis syngas. Thermogravimetric studies revealed that pretreated biochars exhibited significantly lower reactivity with carbon dioxide compared to untreated counterparts, approaching the reactivity of coal. This was attributed to increased aromaticity, C=C bonding, cross-linkages enriching lignin and by the removal of hemicellulose through HTP-SE. Overall, the upgraded biochar addresses key limitations of conventional biochar and shows strong potential as a substitute to replace injection coal entirely in both blast and electric arc furnaces.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133009"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
11C-labeling of 20(S)-protopanaxadiol, an aglycon of ginsenoside, based on the use of Pd(0)-mediated rapid C-[11C]methylation of boronic precursors. 基于Pd(0)介导的硼前体快速C-[11C]甲基化,对20(S)-原人参二醇(人参皂苷的糖基)进行11C标记。
IF 2.2 4区 医学
Bioorganic & Medicinal Chemistry Letters Pub Date : 2025-12-01 Epub Date: 2025-08-05 DOI: 10.1016/j.bmcl.2025.130356
Yoshiki Ooshima, Hiroko Koyama, Aya Ogata, Hiroshi Ikenuma, Takashi Yamada, Hiroyuki Kojima, Takashi Kato, Yasuyuki Kimura, Masaaki Suzuki
{"title":"<sup>11</sup>C-labeling of 20(S)-protopanaxadiol, an aglycon of ginsenoside, based on the use of Pd(0)-mediated rapid C-[<sup>11</sup>C]methylation of boronic precursors.","authors":"Yoshiki Ooshima, Hiroko Koyama, Aya Ogata, Hiroshi Ikenuma, Takashi Yamada, Hiroyuki Kojima, Takashi Kato, Yasuyuki Kimura, Masaaki Suzuki","doi":"10.1016/j.bmcl.2025.130356","DOIUrl":"10.1016/j.bmcl.2025.130356","url":null,"abstract":"<p><p>Ginsenosides, the pharmacologically active components of Panax ginseng, are widely used in herbal medicine and reportedly exert diverse biological effects, including anticancer, anti-inflammatory, and neuroprotective activities. However, their pharmacological mechanisms remain poorly understood, owing to the lack of chemical probes suitable for in vivo analyses. Herein, we report the development of a <sup>11</sup>C radiolabeling of 20(S)-protopanaxadiol (PPD), a major aglycone-type active ginsenoside metabolite, for positron emission tomography (PET) imaging. For the <sup>11</sup>C labeling of PPD, we focused on the terminal vinyl methyl group of the dammarane-type triterpene backbone, a common structural element found in ginsenosides. A boronic precursor applicable for rapid <sup>11</sup>C-methylation was efficiently synthesized via steps focusing on the controlled cross-metathesis of an internal olefin. The subsequent Pd(0)-mediated rapid <sup>11</sup>C-methylation was conducted in N,N-dimethylformamide (DMF)/H₂O using [Pd₂(dba)₃], P(o-tolyl)₃, and sodium ascorbate, which functioned as a base and a radical scavenger. After formulation, the resulting [<sup>11</sup>C]PPD was obtained in a decay-corrected radiochemical yield of 15 ± 2 % (n = 3), with a total radioactivity of 1.0 ± 0.3 GBq (n = 3) and molar activity of 124 ± 7 GBq/μmol (n = 3). Radiochemical purity was ≥99 %, and the total synthesis time was 29 min. Using [<sup>11</sup>C]PPD, PET imaging of the brains of healthy rats and abdomens of healthy mice demonstrated low brain uptake and pronouncedly clear hepatobiliary excretion of radiolabeled species. These findings may provide a foundation for the general labeling of ginsenoside structures with <sup>11</sup>C radioisotopes, thereby enabling systematic in vivo pharmacokinetic analyses of PPD derivatives to advance ginsenoside-based drug development.</p>","PeriodicalId":256,"journal":{"name":"Bioorganic & Medicinal Chemistry Letters","volume":" ","pages":"130356"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144774419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A novel pH-responsive aptamer-molecularly imprinted hydrogel for efficient identification and separation of ampicillin in complex matrices. 一种新型ph响应适体-分子印迹水凝胶,用于复杂基质中氨苄西林的有效鉴定和分离。
IF 6.1 1区 化学
Talanta Pub Date : 2025-12-01 Epub Date: 2025-06-10 DOI: 10.1016/j.talanta.2025.128425
Liyu Qiu, Xixiang Yang, Nan Zhao, Anqi Luo, Li Wang, Xiaogang Hu
{"title":"A novel pH-responsive aptamer-molecularly imprinted hydrogel for efficient identification and separation of ampicillin in complex matrices.","authors":"Liyu Qiu, Xixiang Yang, Nan Zhao, Anqi Luo, Li Wang, Xiaogang Hu","doi":"10.1016/j.talanta.2025.128425","DOIUrl":"10.1016/j.talanta.2025.128425","url":null,"abstract":"<p><p>Hybrids of molecular imprinting-aptamers have been used for environmental analysis; however, the weak selectivity and stability inhibited their applications in complex matrices. In this paper, the nano-scale pH-responsive aptamer-molecularly imprinted interpenetrating hydrogels (Fe<sub>3</sub>O<sub>4</sub>@Apt-MIH) were prepared in one step by the modified sol-gel method based on the preparation principle of interpenetrating network hydrogels, with Fe<sub>3</sub>O<sub>4</sub> as the carrier, aminopropyltriethoxysilane and methyltrimethoxysilane as functional monomers, polyethyleneimine (PEI) as the pH-responsive components, and tetraethoxysilane (TEOS) and glutaraldehyde (GA) as dual cross-linking agents. After optimizing the polymerization and extraction conditions, the pH response performance, extraction capacity and reusability of the material with highly magnetism were investigated, which could achieve magnetic separation within 5 s. The SiO<sub>2</sub> coating hydrolyzed by TEOS provided a rigid backbone for interpenetrating hydrogels, which could improve the acid resistance of PEI by adjusting the isoelectric point of Fe<sub>3</sub>O<sub>4</sub>@Apt-MIH and assist in target identification. Selectivity research showed that aptamers and functional monomers have a highly effective synergistic recognition effect on ampicillin, with the imprinting factor of 6.90 and the synergistic recognition factor of 1.48. This solid-phase microextraction material coupled to high-performance liquid chromatography with the linear range of 10-1000 μg/L and the detection limit of 7.67 μg/L enabled the selective separation and detection of ampicillin in milk and honey, with minimal sample matrix interference and recovery between 95.2 and 102 %.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"295 ","pages":"128425"},"PeriodicalIF":6.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Simple one-pot assay for visual and smartphone-based quantification of human albumin in urine and biological fluids. 简单的一锅试验,用于视觉和智能手机定量人类尿液和生物液体中的白蛋白。
IF 6.1 1区 化学
Talanta Pub Date : 2025-12-01 Epub Date: 2025-06-07 DOI: 10.1016/j.talanta.2025.128443
Nopchulee Cheeveewattanagul, Oranut Warachit, Paskorn Muangphrom, Patsamon Rijiravanich
{"title":"Simple one-pot assay for visual and smartphone-based quantification of human albumin in urine and biological fluids.","authors":"Nopchulee Cheeveewattanagul, Oranut Warachit, Paskorn Muangphrom, Patsamon Rijiravanich","doi":"10.1016/j.talanta.2025.128443","DOIUrl":"10.1016/j.talanta.2025.128443","url":null,"abstract":"<p><p>This study introduces a simple and effective one-pot assay for the quantification of human albumin (HA) using H8 aptamer-modified magnetic beads (H8Apt-MB-HA), applicable across various biological fluids, including urine, serum, and sweat. Upon binding to HA, the aptamer-bead complexes exhibit concentration-dependent aggregation: well-dispersed at low HA levels and clustered at higher concentrations, with rapid sedimentation under a magnetic field in U-shaped well plates. These aggregation patterns are visually discernible, enabling rapid qualitative screening without the need for complex instrumentation. For quantitative analysis, digital images captured by a smartphone or a plate scanner (Cytation™ 5) are processed using ImageJ software, allowing for accurate signal quantification. The top and bottom-view assay achieves a detection limit of 5.9 and 6.5 mg L<sup>-1</sup> and demonstrates robust performance across a urinary pH range of 5.0-7.0. Minimal cross-reactivity with other biological components confirms the aptamer's high specificity. Clinical validation with patient urine samples shows excellent correlation with standard immunoturbidimetric methods, underscoring the assay's potential for point-of-care testing. This approach supports immediate interpretation and clinical decision making, especially valuable in resource-limited settings and for routine monitoring of kidney-related disorders.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"295 ","pages":"128443"},"PeriodicalIF":6.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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