Elsevier最新文献

{"title":"A cascaded tapered seven-core fiber biosensor for rapid detection of the p53 protein in 293T cell lysate.","authors":"Zhichao Zhang, Zefeng Li, Ruen Xie, Zhen Tian, Yicun Yao, Bingwu Yang, Jingao Zhang, Haili Ma, Lan Rao, Kuiru Wang, Binbin Yan, Xinzhu Sang, Chongxiu Yu, Bo Fu, Shengli Pu, Jinhui Yuan, Qiang Wu","doi":"10.1016/j.talanta.2025.128384","DOIUrl":"10.1016/j.talanta.2025.128384","url":null,"abstract":"<p><p>P53 protein is one of the main cancer biomarkers, and its detection is of great significance for early diagnosis and treatment of various cancers such as breast, rectal, and lung cancer. In this paper, a cascaded tapered seven-core fiber (CTSCF) biosensor is proposed for rapid detection of p53 protein. The experiment results show that the proposed CTSCF biosensor can effectively detect the p53 protein at concentrations of 0.05-5 ng/mL, and the limit of detection (LoD) can be as low as 0.0496 ng/mL. Moreover, the ability of the CTSCF biosensor to detect the p53 protein in 293T cell lysate was validated and compared with traditional western blotting (WB) method. The results show that the LoD of the CTSCF biosensor is much lower than that of the WB method. The proposed CTSCF biosensor has rapid detection time, good stability and specificity, high sensitivity, and low LoD, which has great potential for application in clinical medicine and pathophysiology.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"295 ","pages":"128384"},"PeriodicalIF":6.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual role of carbon monoxide in medium-chain fatty acids production from food waste.","authors":"Samuel Gyebi Arhin, Alessandra Cesaro, Francesco Di Capua, Ville Santala, Johanna M Rinta-Kanto, Marika Kokko, Giovanni Esposito","doi":"10.1016/j.biortech.2025.133084","DOIUrl":"10.1016/j.biortech.2025.133084","url":null,"abstract":"<p><p>Upcycling biowaste into useful biochemicals, including medium-chain fatty acids (MCFAs) represents a crucial node in the transition toward a circular economy. However, the output and stability of anaerobic microbiomes for MCFAs production are strongly anchored on the effective inhibition of competing pathways, including methanogenesis, while stimulating the growth of bacteria producing MCFAs. Here, we proposed a mixotrophic chain elongation (CE) concept for producing MCFAs from food waste by exploring carbon monoxide (CO) as a methanogenic inhibitor. Our findings suggest that CO supplementation at an optimum partial pressure (P_CO) of 0.25 atm enhances carbon flux toward MCFAs production instead of methanogenesis, resulting in the highest MCFAs concentration observed in this study (10.4 ± 0.4 g-COD/L), with n-caproate as the predominant MCFA. Experiments with CO as the sole substrate demonstrated that the supplied CO could be converted into intermediates such as acetate and ethanol that potentially augmented MCFAs synthesis. Homoacetogens and chain elongators, notably Megasphaera spp. and members of the Lachnospiraceae family were enriched in the P_CO of 0.25 atm system, forming a putative metabolic network to promote CE. These findings provide insights into valuable MCFAs biosynthesis from biowaste.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133084"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Balancing nitrogen metabolism to efficiently drive anti-tuberculosis ilamycins biosynthesis in Streptomyces atratus.","authors":"Gaofan Zheng, Weiyan Zhou, Yingyue Gui, Yuxi Jiang, Yunfei Zhu, Junying Ma, Jianhua Ju, Xiujuan Xin, Baoli Li, Ruida Wang, Ming Zhao, Faliang An","doi":"10.1016/j.biortech.2025.133099","DOIUrl":"10.1016/j.biortech.2025.133099","url":null,"abstract":"<p><p>The deep-sea-derived Streptomyces atratus SCSIO ZH16 is a promising host for producing nanomole-level anti-tuberculosis ilamycins. However, limited research on regulating the ilamycins biosynthetic gene cluster (BGC) has hindered industrial production. Our previous study found that nitrogen metabolism-related genes were upregulated in strains with enhanced ilamycins production. Since amino acids from nitrogen metabolism are key precursors, we aimed to optimize ilamycins production by balancing BGC expression and nitrogen metabolism. Using RNA-seq and hierarchical clustering, we identified the native promoter P₂₀₆₀₅ and its modified version P_20605-400, which regulate the positive regulator IlaB in ilamycins BGC. To synchronously boost ilamycins synthesis and precursor supply, we analyzed P₂₀₆₀₅'s function via bioinformatics and validated it using an indigoidine biosynthetic model. The engineered strain ΔilaR::P_20605-400-ilaB::P_ermE*-phoP achieved over a dozen-fold increase in ilamycins yield. Fermentation was successfully scaled up in 5-L and 500-L bioreactors, reaching titers of 2,546.4 mg/L and 1,993.9 mg/L, respectively, significantly surpassing previously reported yields. This study highlights the industrial potential of ilamycins and provides insights into enhancing peptide compound production in Streptomyces.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133099"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Near-isothermal hydrothermal carbonization of glucose: Estimating continuous reactor performance from batch kinetics.","authors":"Angesom Aregawi Gebretsadkan, Saeed V Qaramaleki, Cordel G Bever, Yonas Zeslase Belete, Charles J Coronella","doi":"10.1016/j.biortech.2025.133118","DOIUrl":"10.1016/j.biortech.2025.133118","url":null,"abstract":"<p><p>Hydrothermal carbonization (HTC) is a promising thermochemical process for converting biomass into value-added products such as hydrochar. Most HTC research is conducted in batch reactors due to their simplicity, resulting in a lack of studies on continuous operations. Batch reactors have limitations primarily related to a smaller throughput preventing further process development. Continuous reactors, by contrast, offer significant throughput, but design and performance remain largely underexplored. This study investigates the HTC of glucose in both batch and continuous reactors under near-isothermal reaction conditions, with a focus on understanding reaction kinetics. Batch HTC was conducted at 230 °C by injecting a 10 g/L mixture of glucose and water. With use of a sophisticated injection port, biomass reactants were rapidly heated to reaction temperature, allowing for near isothermal reaction conditions. Our approach overcomes the long-time delay resulting from heating a heavy steel reactor. A continuous HTC system was designed, fabricated, and tested with a throughput of 0.30 L/min. The reactor is configured as a baffled plug flow reactor consisting of three main sections: biomass feeding and, the continuous HTC reactor zone, and an energy recovery and depressurization unit. A kinetic model was developed from batch experiments and applied to predict the performance of a continuous HTC reactor using the segregation model. The predicted glucose conversions were 94.80 % for the continuous reactor and 96.30 % for the batch reactor at the same mean residence time of 13.25 min.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133118"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Development of a sustainable approach to produce galactaric acid from sunflower heads by engineered Pseudomonas putida KT2440\" [Bioresour. Technol. 437 (2025) 133115].","authors":"Huanghong Tan, Jie Hou, Jia Ouyang, Zhaojuan Zheng","doi":"10.1016/j.biortech.2025.133179","DOIUrl":"10.1016/j.biortech.2025.133179","url":null,"abstract":"","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"437 ","pages":"133179"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144937620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protocol for virome characterization in low-volume respiratory samples from broiler chickens.","authors":"Giulia Von Tönnemann Pilati, Henrique Borges da Silva Grisard, Rafael Cadamuro Dorighello, Vilmar Benetti Filho, Mariane Dahmer, Beatriz Pereira Savi, Mariana Alves Elois, Gleidson Biasi Carvalho Salles, Eduardo Correa Muniz, Gislaine Fongaro","doi":"10.1016/j.jviromet.2025.115233","DOIUrl":"10.1016/j.jviromet.2025.115233","url":null,"abstract":"<p><p>The poultry industry is a major global source of animal protein but remains vulnerable to immunosuppressive viral infections that compromise bird health and productivity. This study evaluated five viral purification methods for metagenomic analysis of respiratory samples from broiler chickens in Santa Catarina, Brazil. Tracheal swabs from ten flocks (one per farm) were pooled, and 50 µL of a herpes simplex virus type 2 (HSV-2) and murine norovirus (MNV-1) mix was added as an internal positive control. The sample was centrifuged (2000 × g for 30 min), filtered (0.45 μm), and subjected to five purification methods. The filtrate was subjected to five different purification methods. Method 1 (M1) was based on nucleic acid direct genomic extraction of the supernatant. Method 2 (M2): a pre-treatment with DNase was used, followed by genomic extraction. Method 3 (M3) was performed using ultracentrifugation at 100,000 × g / 3 h at 4 °C, followed by genomic extraction. In Method 4 (M4), the sample was submitted to ultracentrifugation on a 25 % sucrose cushion at 100,000 × g / 3 h at 4 °C, followed by genomic extraction. Finally, in Method 5 (M5), the sample was ultracentrifuged on a 25 % sucrose cushion at 100,000 × g / 3 h at 4 °C, and the pellet was treated with DNase followed by genomic extraction. All genomic extractions were performed using the RNeasy Mini kit. Samples were reverse transcribed into cDNA and sequenced by the MiSeq Sequencing System. The efficiency of M1-5 was evaluated based on the yield of viral genetic material. All methodologies employed demonstrated varying rates of genome recovery from viruses identified in poultry production. Notable viruses included avian gyrovirus 2 (AGV-2), avian leukosis virus (ALV), and the avian endogenous retrovirus EAV-HP found within chicken genomes. However, M5 showed the best performance, recovering 9.32 % of viral sequences, 44 % of HSV-2, as internal viral control, 32 % of EAV-HP, 8 % of ALV, and 7 % of AGV-2. In conclusion, this study successfully evaluated and compared five distinct viral purification methods, contributing significantly to the characterization of avian viromes and enhancing comprehension of viral ecology.</p>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":" ","pages":"115233"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144765052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patterns and trends of medication use among patients with bipolar disorder in Northeast China: A study from a large psychiatric center (2013-2022).","authors":"Yu Zhang, Mingyang Yao, Huanliang Li, Yuehua Wang, Shengyuan Hao, Guoliang Pan, Hefeng Xu, Xiaoyu Han, Guangyu Zhou, Liying Shao, Wei Sun, Yan Luan, Guowei Pan","doi":"10.1016/j.jad.2025.119920","DOIUrl":"10.1016/j.jad.2025.119920","url":null,"abstract":"<p><strong>Background: </strong>The medication patterns and trends among bipolar disorder patients in mainland China remain unclear. This study, as the first attempt in northeast China, is designed to clarify the medication patterns and elucidate medication trends of bipolar disorder patients from 2013 to 2022.</p><p><strong>Methods: </strong>The study included 1812 bipolar disorder patients from a large psychiatric center who received treatment from 2013 to 2022. We examined the episode types of patients and medications for treatment of bipolar disorder, including lithium, anticonvulsants, antipsychotics, antidepressants, and sedative-hypnotics. Trends of episode types, medication, and combinations were analyzed using the Cochran-Armitage Trend test. Patterns of medication use were analyzed using the association rule mining method.</p><p><strong>Results: </strong>Among the patients, manic/hypomanic episode accounted for 63.11 % to 75.39 % from 2013 to 2022. Antipsychotics had the highest usage rate (2022: 97.84 %) and showed a significant increasing trend. Lithium had the lowest usage rate (2022: 7.91 %), with no significant trend. Furthermore, combination therapy, particularly three medications, was the major treatment regimen. The proportion of patients receiving combination therapy involving three types of medications ranged from 46.07 % to 64.03 %. The most common pattern for manic/hypomanic patients was sedative-hypnotics + anticonvulsants + antipsychotics, whereas the preferred medication pattern for depressive patients was antipsychotics + antidepressants + sedative-hypnotics.</p><p><strong>Conclusions: </strong>In China, manic/hypomanic episodes remained the predominant episode type. Combination therapy, particularly with three medications, was most commonly used for treatment of bipolar disorder patients and the patterns were episode-specific. Lithium remained the least commonly prescribed medication for both episode types.</p>","PeriodicalId":14963,"journal":{"name":"Journal of affective disorders","volume":" ","pages":"119920"},"PeriodicalIF":4.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of emotional dysregulation and attachment style as indicators of obsessive compulsive symptom-severity.","authors":"Nicole Moriarty, Keith Gaynor, Elif Beyza Koş Yalvaç, Mujgan Inozu","doi":"10.1016/j.jad.2025.119909","DOIUrl":"10.1016/j.jad.2025.119909","url":null,"abstract":"<p><strong>Background: </strong>Existing research has demonstrated an association between obsessive compulsive (OCD) and emotional dysregulation. Additionally, evidence suggests a relationship between OCD and attachment style. The current study (1) investigated if a model made up of emotional dysregulation and related constructs of emotion regulation skills, attachment styles and interpersonal emotion regulation predicted obsessive compulsive symptom severity, and (2) investigated the mediating role of emotional dysregulation between attachment style and OCD in a community sample.</p><p><strong>Methods: </strong>Two hundred and twenty-six participants reported obsessive compulsive symptoms were recruited online and anonymously completed 6 questionnaires. The model was tested through hierarchical linear regressions and mediation analysis. Ethical approval was granted by University College Dublin's Ethics Committee.</p><p><strong>Results: </strong>A hierarchical linear regression employed by the current study indicated that emotional dysregulation and attachment anxiety significantly predicted OCD symptoms (F (5, 1016) = 65.7, p < 0.001). The indirect effect of emotional dysregulation as a mediator of attachment style and OCD symptom-severity was significant (B = 0.4311; 95 % CI: 0.3339 to.5381).</p><p><strong>Limitations: </strong>The current study utilized a cross-sectional design and causation cannot be derived from the results. The use of self-report scales may allow for biased responses and a predominantly Irish sample limits the generalizability of the study.</p><p><strong>Conclusion: </strong>The current study has both clinical and empirical implications as it demonstrates the role of emotional dysregulation and attachment insecurity in OCD severity, potentially advising the direction of future OCD interventions.</p>","PeriodicalId":14963,"journal":{"name":"Journal of affective disorders","volume":" ","pages":"119909"},"PeriodicalIF":4.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Learning to understand an unfamiliar talker: Testing distributional learning as a model of rapid adaptive speech perception.","authors":"Maryann Tan, T Florian Jaeger","doi":"10.1016/j.cognition.2025.106195","DOIUrl":"10.1016/j.cognition.2025.106195","url":null,"abstract":"<p><p>Human speech perception is highly adaptive: exposure to an unfamiliar accent quickly reduces the difficulty listeners might initially experience. How such rapid adaptation unfolds incrementally remains largely unknown. This includes questions about how listeners' prior expectations based on lifelong experiences are integrated with the unfamiliar speech input, as well as questions about the speed and success of adaptation. We begin to address these knowledge gaps through a combination of an incremental exposure-test paradigm and model-guided data interpretation. We expose US English listeners to shifted phonetic distributions of word-initial \"d\" and \"t\" (e.g., \"dill\" vs. \"till\"), while incrementally assessing cumulative changes in listeners' perception. We use Bayesian mixed-effects psychometric models to characterize these changes, and compare listeners' behavior against both idealized learners (ideal observers that know the exposure statistics) and a model of adaptive speech perception (ideal adaptors that have to infer those statistics). We find that a distributional learning model provides a good qualitative and quantitative fit (R²>96%) to both listeners' prior perception and changes in their perception depending on the amount and type of exposure. We do, however, also identify previously unrecognized constraints on adaptivity that are unexpected under any existing model of adaptive speech perception: changes in listeners' perception seem to plateau below the level expected under successful learning.</p>","PeriodicalId":48455,"journal":{"name":"Cognition","volume":"265 ","pages":"106195"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144812594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rehospitalization in youth depression: Critical analysis of predictors and the role of immune markers in long-term management.","authors":"Khairiyah Khadijah, Suryadi Suryadi, Reza Oktiana Akbar, Irman Syahriar, Ria Rizki Agustini, Gugun Gunawan, Siti Aisah, Nuraini Nuraini","doi":"10.1016/j.jad.2025.120060","DOIUrl":"10.1016/j.jad.2025.120060","url":null,"abstract":"","PeriodicalId":14963,"journal":{"name":"Journal of affective disorders","volume":" ","pages":"120060"},"PeriodicalIF":4.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144855236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}