Elsevier最新文献

{"title":"A triple-helix molecular switch-based fluorescent aptasensor using Klenow fragment-assisted target recycling and Ribonuclease H-powered DNA walker cascade amplification for detection of MRSA.","authors":"Jiaxin Xu, Zidong Zhang, Fangguo Lu, Yi Ning","doi":"10.1016/j.talanta.2025.128131","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128131","url":null,"abstract":"<p><p>MRSA is an antibiotic resistant bacterium that poses a significant threat to the environment and human health due to its bioaccumulation and potential widespread contamination. The prompt and accurate identification of MRSA is essential for enhancing environmental monitoring and clinical management. Here, we develop a triple-helix molecular switch (THMS) fluorescent aptasensor for the determination of MRSA using Klenow fragment (KF)-assisted target recycling and Ribonuclease H (Rnase H)-powered DNA walker cascade amplification. In this method, the target opens the THMS by specifically binding with the aptamer, resulting in the release of target/aptamer complex and DNA walker. KF then initiates the target recycling process via strand-displacement polymerization reaction under the assistance of carboxyfluorescein (FAM)-labeled primer and dNTPs, creating plenty of double-stranded DNA (dsDNA) products. These dsDNA products show low affinity to graphene oxide (GO) and generate strong fluorescence. This fluorescence is considerably significantly amplified in the presence of SYBR Green I (SGI), attributable to the synergistic interaction between dsDNA and SGI. In the interim, Rnase H drives the released DNA walker to automatically walk on the carboxylated graphene oxide surface by cleaving FAM-labeled RNA signal probe (SP), causing the FAMs to dissociate from the carboxylated graphene oxide (CGO). Therefore, fluorescent signal originating from the two reaction pathways can be detected at excitation/emission wavelengths of 480/514 nm. The target measured by this strategy demonstrates a broad linear working range from 10² colony-forming units (CFU)/mL to 10⁷ CFU/mL, with a detection limit (LOD) of 15 CFU/mL. Moreover, this method performs well in milk and pus sample analysis. These results reveal that this aptasensor is highly specific and sensitive for detecting MRSA and is endowed with good potential for food monitoring and clinical diagnosis applications.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128131"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Target-initiated autocatalytic and concatenated DNAzyme/CHA amplification cascades for highly sensitive fluorescent detection of TET1 dioxygenase.","authors":"Daxiu Li, Jie Xiang, Yanni Wang, Yun Xiang, Ruo Yuan","doi":"10.1016/j.talanta.2025.128114","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128114","url":null,"abstract":"<p><p>The sensitive detection of the dysregulated expression of ten-eleven translocation 1 (TET1) dioxygenase, a key DNA 5-methylcytosine (5 mC) oxidation regulator in the expression of developmental genes, is of significant importance for the diagnosis of various genetic diseases and cancers. This study describes the establishment of a highly sensitive fluorescent TET1 bioassay based on the 5 mC-modified/Zn²⁺-dependent DNAzyme-containing hairpin probe and the autocatalytic and concatenated DNAzyme/catalytic hairpin assembly (CHA) signal amplification cascades. TET1 target molecules specifically recognize and cut the 5 mC sites in the hairpin probes to release active DNAzyme sequences, which bind and cleave the double-stem-loop substrate strands to trigger multiple concatenated signal amplification recycling cycles with the presence of the fuel strands and two fluorescently quenched signal hairpins. These DNA reaction cascades thus result in the unfolding of lots of signal hairpins to substantially recover fluorescence for highly sensitive TET1 assay with a calculated detection limit of 6.9 fM. Additionally, such bioassay shows high selectivity toward TET1 and its real applicability has been successfully demonstrated for cancer cell lysate and human serum samples.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128114"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multimodal triple-mode probe with colorimetric-fluorescence-SERS (CFSERS) for sensitive and quantitative detection of C-reactive protein in clinical diagnostics.","authors":"Wei Li, Xinrui Wang, Mingze Zhu, Xin Huang, Pacifique Hirwa Umutoni, Ting-Hsuan Chen, Jian Lu, Shih-Chi Chen, Guangming Tan, Bryan P Yan, Bee Luan Khoo","doi":"10.1016/j.talanta.2025.128100","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128100","url":null,"abstract":"<p><p>Chronic inflammation remains a major global health concern, necessitating the development of advanced tools for continuous, precise monitoring. This study introduces a novel, clinically impactful triple-mode probe that integrates colorimetric, fluorescence, and surface-enhanced Raman scattering (SERS) signals, offering unparalleled multimodal detection capabilities. The probe's design leverages europium chelate-doped polystyrene nanoparticles (ECNPs), ensuring minimal signal cross-interference through a long Stokes shift. A key innovation is the development of a multimodal mapping algorithm that seamlessly integrates these optical signals, providing a sensitive and robust platform for biomarker detection with broad dynamic ranges. The probe's clinical relevance is demonstrated by its application in lateral flow assays (LFAs) for detecting C-reactive protein (CRP) levels, achieving a detection limit of 6.31 ng/mL and dynamic ranges from 23.13 to 2000 ng/mL, significantly outperforming single-mode detection methods. In clinical validation using urine samples from 31 patients, the triple-mode LFA showed excellent correlation (0.9377) and agreement (93.55 %) with gold standard enzyme-linked immunosorbent assay (ELISA) results. This demonstrates that the proposed multimodal platform offers a highly sensitive, cost-effective, and versatile tool for monitoring inflammation and other disease biomarkers, with substantial potential for clinical applications in diagnostics and disease management.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128100"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cathepsin B-induced cascade DNA-AuNP nanomachine for activated tumor theranostics.","authors":"Huihui Liu, Limei Xu, Ting Wang, Yingqi Liu, Jiajia Pan, Weiwei Xiong, Fenfen Zheng, Yemei Wang, Shasha Sun","doi":"10.1016/j.talanta.2025.128103","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128103","url":null,"abstract":"<p><p>Since targeted and efficient accumulation of nanoparticles into tumors is essential for accurate cancer theranostics, spatiotemporally controlling the aggregation of small nanoparticles (such as gold nanoparticles, AuNPs) in the tumor microenvironment holds significant promise for improving the diagnostic and therapeutic efficiency against tumors. Here, we introduce a cascade DNA-AuNP nanomachine (CNM) that can in situ magnify the protease-catalyzed peptide cleavage via DNA amplification machinery for cathepsin B (Cat B) activity imaging and Cat B-responsive photothermal therapy of tumors. The CNM is composed of a nanomediator formed by tethering a mediator DNA/peptide complex on AuNPs (DpAuNP) and a nanoeffector consisted of AuNPs and DNA modules (DNA-AuNP). In the cascade, Cat B-mediated peptide cleavage of mediator DNA/peptide complex on DpAuNPs initiates both the detachment of fluorescent DNA reporter from DNA-AuNPs for Cat B imaging and the aggregation of AuNPs for tumor photothermal therapy via toehold-mediated stand displacement (TMSD) reaction. Our results demonstrate that the CNM not only offers superior sensitivity and specificity for Cat B imaging, but also facilitates the activated aggregation of AuNPs for enhanced photothermal therapy of tumors. This CNM represents a Cat B-specific sense-and-treat paradigm for cancer theranostics.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128103"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Hyaluronic acid coating on the surface of curcumin-loaded ZIF-8 nanoparticles for improved breast cancer therapy: An in vitro and in vivo study\" [Colloid. Surf. B Biointerfaces 203 (2021) 111759 /COLSUB-D-20-01957].","authors":"Shaoxuan Yu, Shanyu Wang, Zhike Xie, Shuyan Yu, Ling Li, Haifang Xiao, Yuanda Song","doi":"10.1016/j.colsurfb.2025.114894","DOIUrl":"10.1016/j.colsurfb.2025.114894","url":null,"abstract":"","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":" ","pages":"114894"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Precise photothermal treatment of bacterial infection mediated by charge-switchable nanoplatform with acylsulfonamide betaine surface\" [Colloid. Surf. B: Biointerfaces 245 (2025), 114362].","authors":"Wenyuan Sun, Shumin Hu, Binzhong Lu, Yu Bao, Mengyao Guo, Yingying Yang, Qiuli Cheng, Leitao Zhang, Wenlan Wu, Junbo Li","doi":"10.1016/j.colsurfb.2025.114955","DOIUrl":"10.1016/j.colsurfb.2025.114955","url":null,"abstract":"","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":" ","pages":"114955"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smartphone-integrated ratiometric colorimetric aptasensor for visual detection of ampicillin residue in animal-derived food samples.","authors":"Reena K Sajwan, Pratima R Solanki","doi":"10.1016/j.talanta.2025.128057","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128057","url":null,"abstract":"<p><p>The antibiotic residue in food accelerates bacteria's resistance process that is responsible for serious health issues. A smartphone-integrated ratiometric colorimetric aptasensor has been developed for ampicillin (AMP) detection in milk and egg samples. The sensing phenomenon utilizes the optical and color-changing properties of gold nanoparticles (AuNPs) and specific aptamer with higher affinity towards AMP for its selective detection. The aptasensor shows linear response with AMP concentration 0.06-9.9 μM with a lower detection limit (LOD) of 45.0 nM. The smartphone-integrated color analyzer application was used to identify the solution's red, green, and blue (RGB) color intensity and calculate the G/R ratio of color intensities in the presence of AMP. Aptasensor show linear response with AMP concentration (0-6.6 μM), with a LOD of 53.0 nM. The sensor's results were further validated with liquid chromatography and mass spectroscopy techniques (LC-MS), showing that the developed sensor is suitable for food monitoring and safety applications.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128057"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Immobilization of Candida antarctica lipase B on MWNTs modified by ionic liquids with different functional groups\" [Colloids Surf. B Biointerfaces 160 (2017) 416-422].","authors":"Xiaomei Wan, Xinran Xiang, Susu Tang, Dinghua Yu, He Huang, Yi Hu","doi":"10.1016/j.colsurfb.2025.114921","DOIUrl":"10.1016/j.colsurfb.2025.114921","url":null,"abstract":"","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":" ","pages":"114921"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Copper-manganese bimetallic oxide with excellent laccase-like activity for colorimetric detection of formaldehyde via the specific aldimine condensation reaction.","authors":"Haoyu Chen, Lin Chai, Jialing Gui, Yani Liu, Meiling Liu, Xiaoying Liu, Youyu Zhang, Shouzhuo Yao","doi":"10.1016/j.talanta.2025.128151","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128151","url":null,"abstract":"<p><p>Laccase, known as the \"green catalyst\", holds significant promise for applications in the textile industry and pollutant detection. However, the use of natural laccase is constrained by challenges associated with complex preparation and inherent instability. The emergence of nanozymes provides a pathway for developing laccase-like mimics. This study focuses on the synthesis of copper-manganese oxide (Cu-doped Mn₃O₄) nanoparticles, which demonstrate significant laccase-like activity through the oxidation coupling reaction between 2,4-dichlorophenol (2,4-DCP) and 4-aminoantipyrine (4-AAP) with observable colorimetric change and an obvious absorption peak at 505 nm. Different copper-manganese oxides were synthesized by varying the proportion of metal salts during the synthesis process. A series of studies have demonstrated that copper doping enhances laccase catalytic activity by increasing the oxygen vacancy. Moreover, the introduction of formaldehyde (FA) results in a decrease of the peak at 505 nm, which is attributed to a condensation reaction between the aldehyde and amino groups under neutral conditions. Based on the laccase-like properties of Cu-doped Mn₃O₄, FA detection is achievable within the range of 100 nM to 100 μM, with a low detection limit of 39.4 nM. This research not only presents a novel laccase mimic with a simple synthesis route but also establishes a colorimetric method for FA detection, representing significant progress in food safety applications.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128151"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemiluminescence lateral flow immunosensor using luminol-labeled silver nanoparticles for highly sensitive and quantitative detection of cardiac troponin I.","authors":"Jun Chen, Xuanxu Nan, Li Yang, Yue Cui","doi":"10.1016/j.talanta.2025.128159","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128159","url":null,"abstract":"<p><p>Cardiac troponin I (cTnI) is a highly specific biomarker of cardiomyocyte injury, released during cell disintegration and necrosis, and is the gold standard for diagnosing acute myocardial infarction (AMI). At the onset of AMI, cTnI appears in very low concentrations (pg/mL level), necessitating the development of highly sensitive and rapid detection sensors. In this study, an electrochemiluminescence lateral flow immunosensor (ECL-LFI) was designed using luminol-labeled silver nanoparticles (luminol@AgNPs) for the sensitive and quantitative detection of cTnI. A screen-printed electrode (SPE) was integrated beneath the nitrocellulose (NC) membrane with plastic plates of the same thickness applied on both sides of the SPE to ensure a smooth flow surface. Upon addition of cTnI and the luminol-H₂O₂ system, sandwich immune complexes formed by antibody-functionalized luminol@AgNPs on the strips generated electrochemiluminescent (ECL) signals. The ECL-LFI exhibited a broad linear detection range from 5 pg/mL to 100 ng/mL, with a detection limit as low as 1.6 pg/mL. Additionally, the results show excellent correlation with clinical tests, demonstrating that the ECL-LFI provides a promising point-of-care tool for the early diagnosis of AMI and other diseases.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128159"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}