Elsevier最新文献

{"title":"LexA deficiency confers enhanced thermotolerance and glycogen accumulation in Methylomicrobium buryatense.","authors":"Shuqi Guo, Yamin Jing, Xiaohan Huang, Binghui Liu, Maximilian Lackner, Qiang Fei","doi":"10.1016/j.biortech.2026.135635","DOIUrl":"10.1016/j.biortech.2026.135635","url":null,"abstract":"<p><p>Systematic mining of heat stress factors is pivotal for constructing robust microbial cell factories. However, the genetic basis underlying thermotolerance remains poorly understood in C1 gas-utilizing chassis. Here, we identified LexA as a key regulator governing thermotolerance and glycogen accumulation in the methanotroph Methylotuvimicrobium buryatense 5GB1C. Through ultraviolet mutagenesis coupled with adaptive laboratory evolution, we developed a thermotolerant evolved strain 5C27. Whole-genome resequencing and transcriptomic analysis revealed global transcriptional reprogramming underlying the heat tolerance phenotype. Notably, LexA was sharply downregulated at 40 °C and identified as a core regulatory factor for thermoadaptation. Transcriptomic analysis indicated that the acquired thermotolerance arises from coordinated regulation of oxidative stress response, energy metabolism, and cellular homeostasis. Subsequent validation experiments demonstrated that lexA deficiency simultaneously conferred enhanced thermotolerance and redirected intracellular carbon flux, resulting in increased glycogen production that exceeded wild-type levels even at the optimal growth temperature of 30 °C. Collectively, our work demonstrated for the first time that LexA plays a critical regulatory role in thermotolerance of methanotrophs. This discovery also provides a foundation for advancing the development of thermotolerant cell factories and promotes the deployment of C1-gas industrial biomanufacturing.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135635"},"PeriodicalIF":8.2,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148757702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-entropy multimetallic nitrogen-doped carbon for robust ammonium-ion storage.","authors":"Hucheng Fu, Yiting Xu, Yizhang Yang, Yanchen Chen, Qiuyan Luo, Yiping Xu, Juguo Dai, Dongxu Li, Qiaoyun Qin, Andreu Cabot, Conghui Yuan, Guorong Chen, Lizong Dai","doi":"10.1016/j.jcis.2026.141010","DOIUrl":"10.1016/j.jcis.2026.141010","url":null,"abstract":"<p><p>Ammonium ions (NH₄⁺), owing to their abundance, low cost, and environmental friendliness, have emerged as promising non-metallic charge carriers for next-generation aqueous energy storage systems. However, the lack of suitable electrode materials has so far hindered their practical application. In this study, we report the development of a high-entropy multimetallic electrode material comprising uniformly dispersed Mn, Fe, Co, Ni, and Cu species on a conductive graphitized nitrogen-doped carbon framework. This engineered electrode enables high-performance symmetric NH₄⁺-ion supercapacitors. The multi-metal doping strategy significantly increases the density of electroactive sites, reduces NH₄⁺ diffusion barriers, and enhances structural stability. The optimized electrodes deliver a high specific capacitance of 836 F g^-1 at 1 A g^-1 and demonstrate excellent cycling performance, retaining 97.2% of their initial capacity and nearly 100% Coulombic efficiency after 50,000 cycles in a three-electrode system. Moreover, the assembled symmetric devices achieve energy densities exceeding 53 Wh kg^-1 at a power density of 891 W kg^-1. Density functional theory calculations confirm that the synergistic interaction among multiple metal centers optimizes NH₄⁺ adsorption and charge distribution, accounting for the superior electrochemical performance.</p>","PeriodicalId":351,"journal":{"name":"Journal of Colloid and Interface Science","volume":"723 ","pages":"141010"},"PeriodicalIF":9.6,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148325341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promoting pediatric sleep health in low-resource settings: A specialist's perspective","authors":"Merrill S. Wise","doi":"10.1016/j.sleepx.2025.100166","DOIUrl":"10.1016/j.sleepx.2025.100166","url":null,"abstract":"","PeriodicalId":37065,"journal":{"name":"Sleep Medicine: X","volume":"11 ","pages":"Article 100166"},"PeriodicalIF":0.0,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145749918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to 'Design and development of lab-on-a-centrifuge based on liquid-core waveguide enhancement technology for colorimetric detection of soil Cr(VI) from sample-to-answer' [Talanta 305 (2026), 129673].","authors":"Bingyu Mu, Xing Na, Zhengqin Shi, Zhe Liu, Jixin Liu","doi":"10.1016/j.talanta.2026.130177","DOIUrl":"10.1016/j.talanta.2026.130177","url":null,"abstract":"","PeriodicalId":435,"journal":{"name":"Talanta","volume":" ","pages":"130177"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasensitive, self-calibrating cortisol immunosensor enabled by dual-modal CuMOF-PEI@AuNPs@HRP immunoreceptor.","authors":"Xinyi Wang, Minghao Wang, Wenbin Zhang, Dewang Yan, Liubing Kong, Gaofeng Wang","doi":"10.1016/j.talanta.2026.130164","DOIUrl":"10.1016/j.talanta.2026.130164","url":null,"abstract":"<p><p>Accurate cortisol monitoring is essential for stress assessment and health management, yet reliable quantification remains challenging because signal fluctuations and matrix effects can compromise the performance of conventional single-mode immunosensors. Herein, we developed a fluorescence-colorimetric (FL-CL) dual-modal immunosensor based on a CuMOF-PEI@AuNPs@HRP (CPA) immunoreceptor for cortisol determination. The integrated sensing architecture generates correlated fluorescence and colorimetric responses from the same recognition event, which are combined through a normalized dual-signal output to improve calibration reliability. Under optimized conditions, the proposed sensor exhibited a detection limit of 0.22 ng/mL and a linear range of 3.1-200 ng/mL, covering physiologically relevant cortisol concentrations in saliva. Compared with single-modal assays and commercial kits, the proposed platform demonstrates enhanced sensitivity, improved correlation, good stability, and batch reproducibility. By integrating signal amplification, dual-modal readout, and simplified immunoreceptor fabrication within a single sensing platform, this work provides a reliable strategy for cortisol monitoring and offers potential for extension to other biomarker detection systems.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130164"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148292920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smartphone-integrated bifunctional sensing platform for visual detection of ampicillin and tetracycline in animal manure.","authors":"Ming-Ming Chen, Lu-Chen Cheng, Xiao-Yu Dong, Fang-Jie Zhao, Peng Wang","doi":"10.1016/j.talanta.2026.130173","DOIUrl":"10.1016/j.talanta.2026.130173","url":null,"abstract":"<p><p>Antibiotic residues in environmental matrices pose escalating threats to ecosystems and human health, creating demand for rapid detection. Here, we present a portable smartphone-integrated bifunctional sensor based on Fe-CDs that enables sequential colorimetric and fluorescent detection of ampicillin (Amp) and tetracycline (TC). The Fe-CDs, synthesized via a one-pot hydrothermal process, exhibit stable fluorescence and intrinsic peroxidase-mimicking activity. Ampicillin inhibits the Fe-CDs nanozyme-catalyzed TMB/H₂O₂ chromogenic reaction via •OH consumption, allowing visual colorimetric quantification. Meanwhile, tetracycline strongly quenches the fluorescent signal of Fe-CDs through an inner filter effect, enabling a sensitive fluorescence readout. This bifunctional assay achieves high sensitivity (LOD as low as ∼0.012 μg/mL for Amp and 0.005 μg/mL for TC). By incorporating Fe-CDs onto paper test strips and analyzing with smartphone cameras, the platform allows real-time, rapid quantification of antibiotics in complex matrices. In manure samples, the sensor's results (recoveries ∼94.4-104.8%) agree well with LC-MS/MS measurements, underscoring its accuracy and robustness. Overall, the portable bifunctional Fe-CDs sensor provides a sensitive, field-deployable tool for environmental antibiotic surveillance.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130173"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network structure of psychological resilience, cognitive reappraisal, and social problem-solving in relation to non-suicidal self-injury in a sample of Chinese university freshmen.","authors":"Chengfei Ruan, Xin Chen, Mingyue Wu, Jiangtao Dong, Wei Cui, Dongjun Zhang","doi":"10.1016/j.jad.2026.122387","DOIUrl":"10.1016/j.jad.2026.122387","url":null,"abstract":"<p><strong>Objective: </strong>Non-suicidal self-injury (NSSI) is prevalent among university students and represents a critical public health concern. This cross-sectional study investigated the prevalence of NSSI and its associated factors among freshmen at a university in central China. Using network analysis, we further examined the interrelationships among NSSI and three protective psychological constructs: resilience, cognitive reappraisal, and social problem-solving.</p><p><strong>Methods: </strong>A cross-sectional survey was conducted among 3616 freshmen. After data screening, 2862 valid responses were included in the final analysis. Participants completed validated scales measuring NSSI, cognitive reappraisal, psychological resilience, and social problem-solving. SPSS 27.0 was used to perform logistic regression and group comparisons. Network analysis was performed in R to examine the interrelations and centrality of psychological constructs.</p><p><strong>Results: </strong>The prevalence of NSSI among freshmen was 24.1%. Psychological resilience, cognitive reappraisal, and social problem-solving ability showed a significant stepwise decrease across NSSI severity groups (p < 0.001). Logistic regression showed that cognitive reappraisal, psychological resilience, social problem-solving ability, and rural household registration were negatively associated with NSSI, whereas male gender was positively associated with NSSI. Network analysis further revealed that cognitive reappraisal exhibited relatively high betweenness centrality within the network comprising resilience and social problem-solving subsystems. Within this network, toughness, strength, and avoidant problem-solving showed the highest node strength, whereas strength and negative problem orientation exhibited elevated betweenness centrality.</p><p><strong>Limitations: </strong>The cross-sectional design and the single-institution sample preclude causal inferences and limit the generalizability of the present findings.</p><p><strong>Conclusion: </strong>Cognitive reappraisal, psychological resilience, and social problem-solving ability were independently and negatively correlated with NSSI severity. Network analysis provided complementary insights into the structural relationships among these variables, identifying cognitive reappraisal as a central node within this network.</p>","PeriodicalId":14963,"journal":{"name":"Journal of affective disorders","volume":" ","pages":"122387"},"PeriodicalIF":5.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148760170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sleep quality modifies the association between nocturnal sleep, daytime napping, and mild cognitive impairment: A population-based dose-response study","authors":"Xi Luo ,&nbsp;Zhonghua Yin ,&nbsp;Xueli Sun ,&nbsp;Ke Li","doi":"10.1016/j.sleepx.2026.100175","DOIUrl":"10.1016/j.sleepx.2026.100175","url":null,"abstract":"<div><h3>Objectives</h3><div>This cross-sectional study, based on the two-process model of sleep regulation, explored the nonlinear relationships and interactions among nocturnal sleep duration, daytime napping, sleep quality, and mild cognitive impairment (MCI) in middle-aged and older Chinese adults.</div></div><div><h3>Methods</h3><div>Data from 7480 participants in the 2018 wave of the China Health and Retirement Longitudinal Study (CHARLS) were analyzed. Restricted cubic splines modeled dose-response relationships, and stratified logistic regression assessed subgroup variations. Models were adjusted for sociodemographic, lifestyle, and clinical covariates.</div></div><div><h3>Results</h3><div>The lowest odds of MCI were observed among participants reporting 6–8 h of nocturnal sleep and 30–60 min of daytime napping. Short (&lt;6 h) and long (&gt;8 h) sleep durations, along with prolonged napping (≥90 min), were linked to higher odds of MCI. Sleep quality modified these associations: poor sleep quality elevated the odds of MCI, especially among long sleepers (OR = 1.88 vs. 1.33 for good sleep quality). Among short sleepers (&lt;6 h), 30–60 min naps were associated with lower odds of MCI (OR = 0.72).</div></div><div><h3>Conclusions</h3><div>These findings indicate that, in this cross-sectional sample, 7–8 h of nocturnal sleep combined with 30–60 min of daytime napping were associated with better cognitive status, whereas long sleep with poor sleep quality was associated with higher odds of impairment. These observational associations, considered within a circadian–homeostatic framework, are hypothesis-generating and warrant confirmation in future longitudinal and interventional studies.</div></div>","PeriodicalId":37065,"journal":{"name":"Sleep Medicine: X","volume":"11 ","pages":"Article 100175"},"PeriodicalIF":0.0,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146188382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparing trajectories of cognitive functioning in treatment-resistant and non-resistant depression: a multicentre linear mixed-effects analysis.","authors":"Viktor T H Wahner, Toni Lange, Inga Stonner, Pasquale Paribello, Martina Contu, Marco Pinna, Dobrochna Kopeć, Ewa Ferensztajn-Rochowiak, Filip Rybakowski, Mara Dierssen, Massimo Gennarelli, Mirko Manchia, Alessandra Minelli, Marie-Claude Potier, Claudia Pisanu, Ferran Sanz, Alessio Squassina, Júlia Perera-Bel, Silke Jörgens, Bernhard T Baune","doi":"10.1016/j.jad.2026.122380","DOIUrl":"10.1016/j.jad.2026.122380","url":null,"abstract":"<p><strong>Background: </strong>Impaired cognitive functioning is a severe symptom in major depressive disorder (MDD). Recent evidence suggests it may be a central characteristic in its treatment resistant form (TRD), potentially constituting a clinical marker for treatment resistance and a target amenable to intervention. To date, cognitive functioning in TRD remains poorly understood and longitudinal investigations are scarce.</p><p><strong>Methods: </strong>This observational prospective cohort study, including 320 patients diagnosed with MDD from the multicentre PROMPT study, examined differences in cognitive functioning between 118 TRD and 202 non-TRD patients over a period of twelve weeks in a real-world setting, using linear mixed modelling. Patients that failed to respond to at least two prior antidepressants trials at baseline were classified as TRD.</p><p><strong>Results: </strong>TRD patients showed significantly poorer baseline performances than non-TRD patients in attention/processing speed (β = -0.45; 95%CI[-0.70, -0.19]; FDR-p = 0.003) and verbal memory (β = -0.45; 95%CI[-0.72, -0.18]; FDR-p = 0.003). Significant time × group interactions were observed in motor speed and verbal fluency tasks. Post-hoc-analyses revealed stagnation in TRD patients and significant improvement in non-TRD patients. Across all other tasks improvement was observed in both groups, and random effects showed large heterogeneity between patients, indicating notable individual differences in cognitive performances.</p><p><strong>Conclusions: </strong>The results suggest distinct recovery patters between non-TRD and TRD patients, and diminished functioning in TRD patients at the domain level. However, intact and diminished performances likely occur in both groups, warranting further investigation of cognitive heterogeneity. These short-term findings highlight the need for more comprehensive longitudinal research on cognition in TRD.</p>","PeriodicalId":14963,"journal":{"name":"Journal of affective disorders","volume":" ","pages":"122380"},"PeriodicalIF":5.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148764543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sequence cluster and distal-site engineering of Fdc for efficient isobutene biosynthesis.","authors":"Ting Feng, Xuanyu Cao, Liran Yang, Haoxian Huang, Yanbin Feng, Yunming Fang, Song Xue","doi":"10.1016/j.biortech.2026.135645","DOIUrl":"10.1016/j.biortech.2026.135645","url":null,"abstract":"<p><p>Isobutene is an important platform chemical that is still predominantly produced from petroleum-derived feedstocks. The ferulic acid decarboxylase (Fdc) catalyzed decarboxylation of 3-methylcrotonic acid provides a green and sustainable route to bio-based isobutene. However, the poor activity of native Fdc toward this non-natural substrate severely limits its practical application. Here, we developed an integrated enzyme-engineering strategy that combines sequence-cluster mining with distal-site engineering to improve catalytic performance. The engineered triple-site mutant V132C/Y298F/S484A (3MUT) exhibited 5.3-fold the isobutene production of WT. Coupling this mutant with reaction-process optimization and cofactor engineering increased the isobutene yield to 67.9%, representing the highest reported yield to date. Mechanistic studies revealed that distal-site mutations in 3MUT reshaped the active site by disrupting the M293-mediated hydrogen bond that blocks the substrate to active site, then further promoting favorable hydrogen-bond interactions with the substrate via R183 and E292, respectively. These findings establish an efficient route for sustainable bio-based isobutene production and demonstrate the potential of distal-site engineering for improving UbiD-family decarboxylases.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135645"},"PeriodicalIF":8.2,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}