Elsevier最新文献

{"title":"Development of new PDMS in tube extraction microdevice for enhanced monitoring of polycyclic aromatic hydrocarbons and their derivatives in water.","authors":"Nathan de Souza Coelho, Helvécio Costa Menezes, Zenilda de Lourdes Cardeal","doi":"10.1016/j.talanta.2024.126882","DOIUrl":"10.1016/j.talanta.2024.126882","url":null,"abstract":"<p><p>Contamination by polycyclic aromatic hydrocarbons (PAHs) is an urgent environmental concern, given its atmospheric dispersion and deposition in water bodies and soils. These compounds and their nitrated and oxygenated derivatives, which can exhibit high toxicities, are prioritized in environmental analysis contexts. Amid the demand for precise analytical techniques, comprehensive two-dimensional chromatography coupled with mass spectrometry (GCxGC/Q-TOFMS) has emerged as a promising tool, especially in the face of challenges like co-elution. This study introduces an innovation in the pre-concentration and detection of PAHs using an extraction fiber based on polydimethylsiloxane (PDMS), offering greater robustness and versatility. The proposed technique, termed in-tube extraction, was developed and optimized to effectively retain PAHs and their derivatives in aqueous media, followed by GCxGC/Q-TOFMS determination. Fiber characterization, using techniques such as TG, DTG, FTIR, and SEM, confirmed the hydrophobic compounds retention properties of the PDMS. The determination method was validated, pointing to a significant advancement in the detection and analysis of PAHs in the environment, and proved effective even for traces of these compounds. The results showed that the detection limits (LOD) and quantification limits (LOQ) ranged from 0.07 ng L^-1 to 1.50 ng L^-1 and 0.33 ng L^-1 to 6.65 ng L^-1, respectively; recovery ranged between 72 % and 117 %; and the precision intraday and interday ranged from 1 % to 20 %. The fibers were calibrated in the laboratory, with exposure times for analysis in the equilibrium region ranging from 3 to 10 days. The partition coefficients between PDMS and water were also evaluated, showing logarithm values ranging from 2.78 to 5.98. The fibers were applied to the analysis of real water samples, demonstrating high capacity. Additionally, given the growing demand for sustainable methods, the approach presented here incorporates green chemistry principles, providing an efficient and eco-friendly solution to the current chemical analysis scenario.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rational design of Ag-doped MnFe₂O₄/HNTs for peroxidase-mimicking activity and colorimetric sensing of uric acid in human serum.","authors":"Han Lu, Zhenbo Xiang, Hailong Pang, Qiang Ren","doi":"10.1016/j.talanta.2024.126913","DOIUrl":"10.1016/j.talanta.2024.126913","url":null,"abstract":"<p><p>Mimicking enzyme have significantly advanced sensing assays by replicating native enzyme functions, yet achieving both high catalytic activity and easy recyclability remains a challenge. In this study, Ag-doped MnFe₂O₄/halloysite nanotubes (HNTs) were rationally designed as a novel nanozyme by depositing in-situ Ag and MnFe₂O₄ nanoparticles onto HNTs. The resulting nanocomposite exhibited excellent peroxidase-like activity along with magnetic properties. Leveraging these features, a highly efficient and sensitive colorimetric system for detecting uric acid (UA) was developed. The Ag-doped MnFe₂O₄/HNTs catalyzed the oxidation of 3,3',5,5'-tetramethylbenzidine in the presence of H₂O₂, causing a color change from colorless to blue. The system showed a linear absorbance response to UA concentrations ranging from 1 to 20 μM, with a detection limit of 59 nM. Mechanistic studies revealed that reactive oxygen species intermediates (¹O₂) were generated through the decomposition of H₂O₂, leading to peroxidase-like activity in the Ag-doped MnFe₂O₄/HNTs. The assay was successfully applied to detect UA in human serum with recoveries over 99.68 %. This study indicates the successful application of Ag-doped MnFe₂O₄/HNTs for colorimetric UA detection in human serum. This research introduces a novel approach for designing recyclable, high-performance mimicking enzyme and establishes an effective colorimetric sensing platform for UA detection in human serum.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemiluminescence immunoassay system based on PCN-224-Mn and gold-platinum bimetallic nanoflowers for sensitive detection of ochratoxin A.","authors":"Yue Kong, Xinyue Qian, Xiao Mei, Jun Ma, Kang Wu, Anping Deng, Jianguo Li","doi":"10.1016/j.talanta.2024.126937","DOIUrl":"10.1016/j.talanta.2024.126937","url":null,"abstract":"<p><p>In this work, a novel Electrochemiluminescence Immunosensor was constructed using PCN-224-Mn and gold-platinum nanoflowers (AuPt NFs) for the ultrasensitive detection of ochratoxin A (OTA). PCN-224 modified with Mn (II) was synthesized as a probe material. The interaction efficiency of PCN-224 with S₂O₈^2- was also greatly improved. AuPt NFs were used as the substrate material for the electrodes. It has favorable biocompatibility, large specific surface area and can bind more antigen. Also greatly increased the electroactive surface area and conductivity of the electrode. OTA was detected using a competitive immunoassay strategy, in which OTA in the sample competes with the encapsulated antigen for a finite number of antibodies. ECLIA for the detection of OTA was designed to be highly sensitive, with a linear range from 0.0002 ng mL^-1 to 1000 ng mL^-1 and a LOD as low as 0.067 pg mL^-1. In addition, it was evident from the electrochemical analyses that PCN-224-Mn had a stronger and more stable ECL signal compared to the plain PCN-224. The successful preparation of specific, sensitive and reproducible ECL immunosensors confirms the great promise for the detection of OTA or other small molecule mycotoxins.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated identification and detection of hydration state and its evolution using terahertz technology.","authors":"Rong Zhao, Qiang Niu, Ghulam Murtaza, Guling Zhang, Yuping Yang","doi":"10.1016/j.talanta.2024.126943","DOIUrl":"10.1016/j.talanta.2024.126943","url":null,"abstract":"<p><p>The accurate detection of dehydration processes in hydrated drugs can reveal various intermolecular vibration modes mediated by hydrogen bonds between water molecules and other components, which underpin the further development of pharmaceutical science, food safety and biophysics. Herein, terahertz (THz) technology is utilized to investigate the dehydration state of d(+)-Raffinose pentahydrate (Rf·5H₂O), in conjunction with imaging-based point by point scanning data acquisition and barcodes methods, to establish an innovative platform integrated identification, trace detection, and application capabilities. Our study demonstrates that the dehydration process of Rf·5H₂O can be dynamically monitored through the evolution of its THz absorption peaks, offering more precise results compared to XRD and Raman spectroscopies. Moreover, the absorbance spectra data collected at each individual pixel is utilized to build visualized THz images, achieving an ultralow minimum content required for detection of 0.032 μg/(50 μm)². Additionally, we introduce a THz spectra-barcode conversion system that not only ensures efficient electronic recordkeeping but also enhances user readability, thereby facilitating the practical applications of THz technology.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A skin-mountable flexible biosensor based on Cu-MOF/PEDOT composites for sweat ascorbic acid monitoring.","authors":"Wei Ling, Xue Shang, Junchen Liu, Tao Tang","doi":"10.1016/j.bios.2024.116852","DOIUrl":"10.1016/j.bios.2024.116852","url":null,"abstract":"<p><p>Continuous monitoring of sweat nutrients offers valuable insights into metabolic cycling and health levels. However, existing methods often lack adaptability and real-time capabilities. Here, we propose a skin-mountable flexible biosensor integrated with metal-organic framework (MOF)-derived composites for real-time monitoring of sweat ascorbic acid (AA) levels. The biosensor features a miniaturized, highly integrated system capable of an imperceptible, stretchable skin patch with dimensions of 16.9 × 9.9 × 0.1 mm³, ensuring conformal integration with curvilinear skin contours. The introduction of a copper-based MOF anchored with poly(3,4-ethylenedioxythiophene) (Cu-MOF/PEDOT) significantly enhances sensing performance toward AA, achieving a detection limit of 0.76 μM and a sensitivity of 725.7 μA/(mM·cm²). Moreover, a miniaturized flexible circuit enables wireless communication, resulting in a lightweight, wearable platform weighing only 1.3 g. Structural and electrochemical analyses confirm the favorable sensitivity, reversibility, and stability of the biosensor, while in-vivo validation in human subjects further reveals the capability to track sweat AA variations during nutrient intake and sustained exercise, showcasing its potential in metabolic cycle assessment and health management. The biosensor presents a promising avenue for scalable health monitoring using adaptable and user-friendly technologies.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Capillary flow velocity-based length identification of PCR and RPA products on paper microfluidic chips.","authors":"Bailey C Buchanan, Reid S Loeffler, Rongguang Liang, Jeong-Yeol Yoon","doi":"10.1016/j.bios.2024.116861","DOIUrl":"10.1016/j.bios.2024.116861","url":null,"abstract":"<p><p>This work demonstrates a novel, non-fluorescence approach to the length identification of polymerase chain reaction (PCR) and recombinase polymerase amplification (RPA) products, utilizing capillary flow velocities on paper microfluidic chips. It required only a blank paper chip, aminated microspheres, and a smartphone, with a rapid assay time and under ambient lighting. A smartphone evaluated the initial capillary flow velocities on the paper chips for the PCR and RPA products from various bacterial samples, where the pre-loaded aminated microspheres differentiated their flow velocities. Flow velocities were analyzed at different time frames and compared with the instantaneous flow velocities and interfacial tension (γ_LV) data. Subsequent error analysis justified the use of the initial time frames. A robust linear relationship could be established between the initial flow velocities against the square root of the product lengths, with R² values of 0.981 for PCR and 0.993 for RPA. The assay seemed not to have a significant dependency on the cycle numbers and initial target concentrations. This novel method can be potentially used with various paper microfluidic methods of nucleic acid amplification tests towards rapid and handheld assays.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11543505/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A semiconductor SERS sensor of corrosion-resistant PPy/GO composite film by electrochemical growth for detecting crystal violet residues in fresh fish tissue.","authors":"Xishun Peng, Qixin Chen, Xinghua Li, Li Yang, Yiheng Yuan, Cheng Zuo, Zhangyu Zhou, Zhongchen Bai","doi":"10.1016/j.talanta.2024.126906","DOIUrl":"10.1016/j.talanta.2024.126906","url":null,"abstract":"<p><p>Crystal violet (CV) residues in Marine food have produced a severe health threat in human life. In this study, we proposed a semiconductor surface-enhanced Raman scattering (SERS) sensor of corrosion-resistant Polyaniline/Graphene oxide (PPy/GO) film by electrochemical growth method to detect CV residues in fresh fish tissue. A PPy/GO dispersion solution was one-step deposited on a stainless steel sheet surface by electrochemical polymerization process to form a PPy/GO composite film acting as a semiconductor SERS substrate. Since the substrate of PPy/GO film was mainly composed of GO sheet without other metals, it had a good corrosion resistance. The SERS enhancement factor and charge transfer intensity P_CT of PPy/Go SERS substrate for CV molecules were up to 1.18 × 10⁶ and 0.903, respectively. Furthermore, the limit of detection (LOD) of PPy/GO SERS substrate could reach 1.58 nM. In addition, SERS sensor of PPy/GO film could identify CV residues in fresh fish tissues, and its recovery rate was 91.8 %-107 %. This preparing method and detecting method we proposed PPy/GO SERS substrate provide a new pathway for detecting CV residues in Marine food.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical sensor featuring PdFeCo_1-xONPs on carbon paper for the sensitive determination of indole-3-lactic acid levels in serum samples.","authors":"Dieudonne Tanue Nde, Dhananjaya Merum, Gyawali Ghanashyam, Jean Pierre Ndabakuranye, Mohamed A Habila, Arghya Narayan Banerjee, Sang Woo Joo, Theophile Niyitanga, Haekyoung Kim","doi":"10.1016/j.talanta.2024.126919","DOIUrl":"10.1016/j.talanta.2024.126919","url":null,"abstract":"<p><p>A highly sensitive and selective electrochemical sensing platform with self-assembled porous 3-D trimetallic (Pd, Fe, and Co) hybrid anchored on a cost-effective and high-conducting carbon paper (CP) synthesized via a facile and cost-effective hydrothermal impregnation and thermal reduction technique was developed for determining indole-3-lactic acid (ILA) levels in buffer and serum samples. Before the analytical phase, the composite (PdFeCo_1-xONPs@CP electrode) was thoroughly characterized, and different methods were used to investigate the electrochemical properties. The combination of tri-metallics with CP-fibers improved sensing capacities in the linear range of 0.05-30 μM, with sensitivity and limits of detection of and 0.165 ± 0.013 μA/μM and 7.8 ± 0. 2 nM, respectively, towards ILA determination. Furthermore, the developed sensing platform was utilized for the analyses of ILA in sigma, human normal, and alcohol use disorder patients' serum samples. Liquid chromatography in tandem with mass spectrometry was equally used to quantify ILA levels in the serum samples and the results of both methods were compared.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ratiometric fluorescence sensing and discrimination of tetracycline analogs by using coumarin-embedded Eu-MOF nanosensor.","authors":"Peng-Xiang Yuan, Yu-Ping Wang, Fangfang Du, Liu-Pan Yang, Li-Li Wang","doi":"10.1016/j.talanta.2024.126914","DOIUrl":"10.1016/j.talanta.2024.126914","url":null,"abstract":"<p><p>As widely used antibiotics, tetracycline residues exist in food and environmental media, which pose certain hidden dangers and negative effects on public health. Therefore, the sensing and discrimination of tetracycline analogs (TCs) have great significance for improving food safety and preventing environmental pollution. Herein, a 7-hydroxycoumarin-3-carboxylic acid-embedded Eu-MOF (HC@Eu-MOF) material was constructed and then developed for the detection of TCs. Upon addition of TCs, the synthesized sensor displays opposite fluorescence changes at two different wavelengths due to the simultaneous presence of the inner filter effect (IFE) and the antenna effect (AE), and achieves a stable ratio signal response within 90 s. In addition, six important tetracycline analogs, including chlortetracycline (CTC), oxytetracycline (OTC), tetracycline (TC), metacycline (MC), doxycycline (DC) and demeclocycline (DMC) can be discriminated with 100 % accuracy through the principal component analysis even in extremely complicated mixtures. Further, a smartphone-assisted portable device was applied for visual sensing of TCs. The as-developed platform possessed the characteristics of simple synthesis, fast response, high sensitivity, and high stability, which further lays a further foundation for the on-site visual detection and discrimination of TCs in real samples.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection of DNA aptamers against Chikungunya virus Envelope 2 Protein and their application in sandwich ELASA.","authors":"Anna Andrew, Magdline S H Sum, Ewe Seng Ch'ng, Thean-Hock Tang, Marimuthu Citartan","doi":"10.1016/j.talanta.2024.126842","DOIUrl":"10.1016/j.talanta.2024.126842","url":null,"abstract":"<p><p>Chikungunya fever, caused by Chikungunya virus (CHIKV) exhibits clinical features that mimic that of other arbovirus infections such as dengue. CHIKV Envelope 2 (E2) protein, an antigenic epitope of CHIKV, has been identified as an ideal marker for diagnostics. The current CHIKV antigen detection tests are largely based on antibodies but are beleaguered by issues such as sensitivity to high temperature, expensive and prone to batch-to-batch variations. Aptamers are suitable alternatives to antibodies as they are cheaper and have no batch-to-batch variations compared to antibodies. In this study, DNA aptamer selection against CHIKV E2 proteins was performed using two different randomized ssDNA libraries. Chik-2 (96-mer) and Chik-3 (76-mer) were isolated from these two libraries and were identified as the potential aptamers against CHIKV E2 protein. The binding affinity of Chik-2 and Chik-3 against CHIKV E2 protein was estimated at 177.5 ± 32.69 nM and 30.01 ± 3.60 nM, respectively. A sandwich ELASA was developed, and this assay showed a detection limit of 2.17 x 10³ PFU/mL. The sensitivity and specificity of the assay were 80 % and 100 %, respectively. The assay showed no cross-reactivity with dengue-positive samples, demonstrating the enormous diagnostic potential of these aptamers for the detection of CHIKV.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}