A smartphone sensing colorimetric detection of ascorbic acid in fruit samples based on Cu₂Fe(CN)₆@PVP nanozyme.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-10-01 Epub Date: 2025-04-14 DOI:10.1016/j.talanta.2025.128152

Xinyu Zhang, Xuan Zhou, Quan Wan, Taolei Sun, Guanbin Gao

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引用次数: 0

Abstract

Prussian blue analogue nanozymes (PBANZs) have been widely employed in the colorimetric detection of various organic small molecules due to their peroxidase-like activity. However, the practical applications of PBANZ are limited by its narrow operating pH range, insufficient catalytic activity, and poor stability. In this study, we synthesized a novel Cu₂Fe(CN)₆@PVP PBA nanozyme (Cu-PBANZ) using polyvinylpyrrolidone (PVP) as a capping ligand. This novel Cu-PBANZ not only exhibited enhanced peroxidase-like activity and improved stability under near-neutral conditions but also demonstrated higher sensitivity and a broader linear range in the detection of hydrogen peroxide (20-4000 μM, LOD 5 μM) and ascorbic acid (10-2000 μM, LOD 3.9 μM). Besides, a smartphone-based platform was developed for detecting AA content in fruits, achieving a detection limit of 7.8 μM. Both UV-Vis spectrophotometry and smartphone-based colorimetry were validated with recovery rates of 95.1 %-106.7 % and 94.8 %-108.9 %, respectively. This study proposed a promising biosensor incorporating Cu-PBANZ for rapid colorimetric detection of AA in fruits using a smartphone.

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基于Cu2Fe(CN)6@PVP纳米酶的智能手机感应比色法检测水果样品中的抗坏血酸。

普鲁士蓝类似物纳米酶（PBANZs）由于其类似过氧化物酶的活性而广泛应用于各种有机小分子的比色检测中。然而，PBANZ的实际应用受到其工作pH范围窄、催化活性不足和稳定性差的限制。本研究以聚乙烯吡咯烷酮（PVP）为盖层配体，合成了一种新型Cu2Fe(CN)6@PVP PBA纳米酶（Cu-PBANZ）。该新型Cu-PBANZ不仅在近中性条件下表现出更强的过氧化物酶样活性和稳定性，而且在过氧化氢（20-4000 μM, LOD 5 μM）和抗坏血酸（10-2000 μM, LOD 3.9 μM）检测中具有更高的灵敏度和更宽的线性范围。建立了基于智能手机的水果AA含量检测平台，检测限为7.8 μM。紫外可见分光光度法和智能手机比色法的回收率分别为95.1% ~ 106.7%和94.8% ~ 108.9%。本研究提出了一种基于Cu-PBANZ的生物传感器，用于智能手机水果中AA的快速比色检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.