Therapeutic Potential of Rose Hip-Derived Nanoparticles for Psoriatic Skin Inflammation.

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Biomaterials Science & Engineering Pub Date : 2025-09-25 DOI:10.1021/acsbiomaterials.5c00826

Masahiro Hashimoto, Shoko Itakura, Kosuke Kusamori, Katsuhiko Yajima, Shota Mitsuhashi, Shinichiro Hayashi, Hiroaki Todo, Makiya Nishikawa

{"title":"Therapeutic Potential of Rose Hip-Derived Nanoparticles for Psoriatic Skin Inflammation.","authors":"Masahiro Hashimoto, Shoko Itakura, Kosuke Kusamori, Katsuhiko Yajima, Shota Mitsuhashi, Shinichiro Hayashi, Hiroaki Todo, Makiya Nishikawa","doi":"10.1021/acsbiomaterials.5c00826","DOIUrl":null,"url":null,"abstract":"<p><p>Psoriasis is a chronic skin disease characterized by hyperproliferation of keratinocytes and excessive inflammation. Plant-derived nanoparticles (pdNPs) are promising agents for treating inflammatory skin diseases. In this study, we examined the characteristics and functions of rose hip-derived nanoparticles (RNPs) rich in various bioactive compounds. RNPs were isolated from rose hips using sucrose ultracentrifugation and characterized using NanoSight and transmission electron microscopy. Cellular uptake by HaCaT human keratinocytes was analyzed using flow cytometry and confocal microscopy. Uptake mechanisms were investigated using siRNA knockdown. Proliferation, apoptosis, and cytokine expression were evaluated in a HaCaT psoriasis model. Antioxidant activity was assessed by measuring reactive oxygen species (ROS) levels in stimulated HaCaT and RAW264.7 mouse macrophage-like cells. The in vivo efficacy was evaluated in a mouse model of psoriasis via intradermal injection of RNPs. The RNPs obtained via ultracentrifugation exhibited a vesicular structure of approximately 100 nm in diameter. They were efficiently taken up by HaCaT cells and inhibited excessive inflammation-induced proliferation. RNPs reduced the mRNA levels of the inflammatory cytokines, interleukin-1β and interferon-γ. Additionally, RNPs were efficiently internalized by mouse macrophage-like RAW264.7 cells, decreasing the intracellular reactive oxygen species levels. The intradermal injection of RNPs effectively suppressed epidermal hyperproliferation and macrophage infiltration in an imiquimod-induced psoriasis mouse model. Collectively, these results suggest that RNPs can be used to treat psoriasis by regulating oxidative stress and inhibiting epidermal hyperproliferation. RNPs, which exerted potent effects on epidermal cells, target key pathological mechanisms such as oxidative stress and immune-driven keratinocyte proliferation. Therefore, they are promising natural therapeutic agents for psoriasis.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.5000,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Biomaterials Science & Engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1021/acsbiomaterials.5c00826","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}

引用次数: 0

Abstract

Psoriasis is a chronic skin disease characterized by hyperproliferation of keratinocytes and excessive inflammation. Plant-derived nanoparticles (pdNPs) are promising agents for treating inflammatory skin diseases. In this study, we examined the characteristics and functions of rose hip-derived nanoparticles (RNPs) rich in various bioactive compounds. RNPs were isolated from rose hips using sucrose ultracentrifugation and characterized using NanoSight and transmission electron microscopy. Cellular uptake by HaCaT human keratinocytes was analyzed using flow cytometry and confocal microscopy. Uptake mechanisms were investigated using siRNA knockdown. Proliferation, apoptosis, and cytokine expression were evaluated in a HaCaT psoriasis model. Antioxidant activity was assessed by measuring reactive oxygen species (ROS) levels in stimulated HaCaT and RAW264.7 mouse macrophage-like cells. The in vivo efficacy was evaluated in a mouse model of psoriasis via intradermal injection of RNPs. The RNPs obtained via ultracentrifugation exhibited a vesicular structure of approximately 100 nm in diameter. They were efficiently taken up by HaCaT cells and inhibited excessive inflammation-induced proliferation. RNPs reduced the mRNA levels of the inflammatory cytokines, interleukin-1β and interferon-γ. Additionally, RNPs were efficiently internalized by mouse macrophage-like RAW264.7 cells, decreasing the intracellular reactive oxygen species levels. The intradermal injection of RNPs effectively suppressed epidermal hyperproliferation and macrophage infiltration in an imiquimod-induced psoriasis mouse model. Collectively, these results suggest that RNPs can be used to treat psoriasis by regulating oxidative stress and inhibiting epidermal hyperproliferation. RNPs, which exerted potent effects on epidermal cells, target key pathological mechanisms such as oxidative stress and immune-driven keratinocyte proliferation. Therefore, they are promising natural therapeutic agents for psoriasis.

查看原文本刊更多论文

玫瑰臀部衍生纳米颗粒治疗银屑病皮肤炎症的潜力。

银屑病是一种慢性皮肤病，以角化细胞过度增生和过度炎症为特征。植物源性纳米颗粒（pdNPs）是治疗炎症性皮肤病的有前途的药物。在本研究中，我们研究了富含多种生物活性化合物的玫瑰籽源纳米颗粒（RNPs）的特性和功能。利用蔗糖超离心技术从玫瑰果中分离RNPs，并用纳米显微镜和透射电镜对其进行了表征。用流式细胞术和共聚焦显微镜分析HaCaT人角质形成细胞的细胞摄取。通过敲低siRNA来研究摄取机制。在HaCaT银屑病模型中评估增殖、细胞凋亡和细胞因子表达。通过测定HaCaT和RAW264.7小鼠巨噬细胞样细胞的活性氧（ROS）水平来评估抗氧化活性。在牛皮癣小鼠模型中，通过皮内注射RNPs来评估体内疗效。通过超离心获得的RNPs显示出直径约100 nm的囊泡结构。它们被HaCaT细胞有效吸收，抑制过度炎症诱导的增殖。RNPs降低炎症细胞因子、白细胞介素-1β和干扰素-γ的mRNA水平。此外，RNPs被小鼠巨噬细胞样RAW264.7细胞有效内化，降低细胞内活性氧水平。在吡喹莫德诱导的银屑病小鼠模型中，皮内注射RNPs可有效抑制表皮增生和巨噬细胞浸润。总之，这些结果表明RNPs可以通过调节氧化应激和抑制表皮过度增生来治疗牛皮癣。RNPs对表皮细胞发挥着强大的作用，其目标是氧化应激和免疫驱动的角质细胞增殖等关键病理机制。因此，它们是有希望的天然治疗银屑病的药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

期刊介绍： ACS Biomaterials Science & Engineering is the leading journal in the field of biomaterials, serving as an international forum for publishing cutting-edge research and innovative ideas on a broad range of topics: Applications and Health – implantable tissues and devices, prosthesis, health risks, toxicology Bio-interactions and Bio-compatibility – material-biology interactions, chemical/morphological/structural communication, mechanobiology, signaling and biological responses, immuno-engineering, calcification, coatings, corrosion and degradation of biomaterials and devices, biophysical regulation of cell functions Characterization, Synthesis, and Modification – new biomaterials, bioinspired and biomimetic approaches to biomaterials, exploiting structural hierarchy and architectural control, combinatorial strategies for biomaterials discovery, genetic biomaterials design, synthetic biology, new composite systems, bionics, polymer synthesis Controlled Release and Delivery Systems – biomaterial-based drug and gene delivery, bio-responsive delivery of regulatory molecules, pharmaceutical engineering Healthcare Advances – clinical translation, regulatory issues, patient safety, emerging trends Imaging and Diagnostics – imaging agents and probes, theranostics, biosensors, monitoring Manufacturing and Technology – 3D printing, inks, organ-on-a-chip, bioreactor/perfusion systems, microdevices, BioMEMS, optics and electronics interfaces with biomaterials, systems integration Modeling and Informatics Tools – scaling methods to guide biomaterial design, predictive algorithms for structure-function, biomechanics, integrating bioinformatics with biomaterials discovery, metabolomics in the context of biomaterials Tissue Engineering and Regenerative Medicine – basic and applied studies, cell therapies, scaffolds, vascularization, bioartificial organs, transplantation and functionality, cellular agriculture