Lab on a Chip最新文献

{"title":"Real-time monitoring of moist wound healing by a flexible adaptive impedance sensing patch.","authors":"Runzhi Yang, Shanshan Wang, Guomin Liang, Yating Zhang, Huiting Zhao, Ting Zou, Xiao Han, Qin Li, Zewen Wei, Gang Wang","doi":"10.1039/d6lc00270f","DOIUrl":"10.1039/d6lc00270f","url":null,"abstract":"<p><p>Moist wound healing has been recommended by the U.S. Food and Drug Administration (FDA) as a preferred approach for skin wound treatment. The global market for moist wound dressings is valued at approximately USD 4.83 billion, driven by the ability of moist wound environments to promote faster healing, improve recovery quality, and reduce pain. Covering the wound with dressings, mostly hydrocolloid-based, is a preferred method to maintain a moist wound environment and isolate this environment from external interferences. Currently, commercial hydrocolloid dressings have already reached satisfactory performance, including efficient exudate absorption, good bacterial control, and low cost. Meanwhile, these commercial hydrocolloid dressings are opaque or will become opaque after absorbing liquid, hindering the direct observation of the wound. Under such circumstances, possible infections or unsatisfactory wound recovery cannot be discovered in time and may deteriorate the wound. To address this issue, this study presents a simple, wireless, and lightweight (3.2 g) impedance sensor for monitoring wound healing status under commercial hydrocolloid dressings. The specially designed spiral parallel electrodes realize accurate impedance measuring and, more importantly, adapt to the gradually contracting wound site. The adaptive moist wound sensing patch (AMoW-SP) substrate provides both high flexibility to adapt to the skin profile and good biocompatibility for not interfering with the wound recovery. The compact wireless communication and power circuit enables visual readout of the wound recovery status on a smartphone and therefore maintains the point-of-care nature of hydrocolloid-dressing-based moist healing. Mouse experiments demonstrate that the AMoW-SP is capable of accurately monitoring normal and chronic wounds for 24 days.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":"5075-5087"},"PeriodicalIF":6.3,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148808078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design-Dependent Shear and Liquid Media Transport Characteristics in Microfluidic U-Shaped Cell Traps","authors":"Louise Lacroix,Emilia Drescher,Christoph Noack,Irene Fernandez-Cuesta","doi":"10.1039/d6lc00625f","DOIUrl":"https://doi.org/10.1039/d6lc00625f","url":null,"abstract":"Single-cell analysis has become central to fields ranging from cancer biology to drug screening. To exploit its potential, tools that can isolate individual cells and maintain them under controlled conditions are needed. Microfluidic platforms are an ideal fit for this, offering precise control over microenvironments. In these systems, cell behavior is shaped by parameters such as fluid shear stress (the mechanical force exerted by flow) and the transport of soluble factors (nutrients and signaling molecules). However, both are set by flow velocity, so they cannot be tuned independently. Here, we present a systematic characterization of the fluid mechanical environment in microfluidic devices featuring U-shaped traps in two distinct designs: slit traps, employing vertical slits to retain cells while maintaining lateral flow through the slits, and gap traps, using a shallow bottom gap to allow vertical flow beneath the cells. Using microparticle tracking velocimetry in microfabricated fluidic chips combined with COMSOL Multiphysics simulations, we quantify shear rates and Péclet numbers across a wide range of trap geometries and pump flow rates. Shear stress scales linearly with the applied flow rate in all configurations, accessing biologically relevant shear stress levels while mainly remaining below the threshold for adverse cellular responses. The Péclet number increases exponentially with the slit/gap size for both trap types. We further show that capture efficiency scales with slit or gap size but is independent of pump flow rate, offering an additional, orthogonal design parameter. Furthermore, we demonstrate successful trapping of cells from different cell lines in the different device types. The presented work provides a quantitative framework for the design of trap geometry and flow rate to achieve optimal cell culture conditions in microfluidic chips.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"19 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148957898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Deep Learning-Enhanced Surface Acoustic Wave Platform for Contact-Free In Situ Biomechanical Characterization of Embryos","authors":"Yupeng Yang,Jinming Chen,Yi Ding,Jiguang Han,Chunhua Wang,Lingyun Li,Lingzhi Cao,Jun Wei,Zengguang Hou,Zenan Wang","doi":"10.1039/d5lc01196e","DOIUrl":"https://doi.org/10.1039/d5lc01196e","url":null,"abstract":"Understanding the mechanical strength of embryos is essential for evaluating their viability in developmental biology. This study introduces a novel biosensor designed for in situ, non-contact mechanical characterization. The platform offers several unique contributions, including: (1) a microfluidic system combining SAW and deep learning for the automated quantification of embryonic mechanical strength; (2) a quantitative fitting model relating SAW power to acoustic radiation force for precise mechanical loading; (3) the first quantitative atlas of embryonic mechanical thresholds, achieved through the systematic measurement of two key parameters: the apparent maximum compressive stress (MCS) causing developmental arrest and the apparent ultimate compressive strength (UCS) leading to embryonic rupture. The results demonstrate consistent embryonic strengthening from zygote to pharyngula stages, with MCS increasing from 8.113 kPa to 11.407 kPa and UCS from 10.256 kPa to 14.219 kPa. This automated platform advances the understanding of embryonic structural integrity for developmental biology and tissue engineering.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"19 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148957887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sliding rail-enabled capillary flow control on μPADs for programmable fluid delivery","authors":"Yixi Shi,Jiahua Zhong,Haonan Li,Hao Chen,Huiru Zhang,Lok Ting CHU,Meiai Lin,Weijin Guo","doi":"10.1039/d6lc00553e","DOIUrl":"https://doi.org/10.1039/d6lc00553e","url":null,"abstract":"Precise control of fluid transport is critical for paper-based microfluidic analytical devices (μPADs), especially in point-of-care testing (POCT) applications involving multistep reactions and timed reagent delivery. Here, we propose a simple yet versatile strategy to regulate capillary flow on μPADs by overlaying a film above a paper strip as a sliding rail for liquid samples. By systematically investigating the surface wettability of the sliding rail and its distance (0.1-2.4 mm) from the NC paper strip, we demonstrate that flow velocity on μPADs can be efficiently modulated through geometric control and surface modification. The influence of the width of sliding rails is also investigated. For hydrophilic sliding rails, increasing the width of film will accelerate flow velocity, whereas for hydrophobic sliding rails, narrower film widths yield higher flow velocity. Furthermore, we use a three-branch μPAD to show that both sequential and synchronized fluid arrival can be achieved by fine-tuning gap distance and paper strip width. In addition, a proof-of-concept delay valve by introducing a continuous hydrophilic sliding rail above segmented NC paper strips is successfully validated. A 1-mm interval located at various positions of μPADs will lead to different time delays. Beyond flow control, the sliding rail also enables localized droplet delivery and transport using a Janus hydrophobic-hydrophilic film with defined apertures. This platform supports sequential and parallel reagent delivery, allowing chemical reactions on-chip. The feasibility of this approach is validated through precipitation reactions, colorimetric reactions, and an enzymatic colorimetric glucose assay, which exhibits a strong correlation (R2 = 0.9980) between gray intensity and glucose concentration under controlled laboratory conditions, suggesting its potential for quantitative applications in high-concentration samples. Overall, this work introduces a highly adaptable and fabrication-friendly μPAD platform with rapid flow tuning and multifunctional reaction capability. The mechanical support and alignment structure (3D-printed frame and spacers) are reusable across experiments, while the paper strips and surface-modified films are single-use consumables to ensure reproducible flow performance. This offers a powerful solution for efficient POCT applications and paper-based microfluidic systems.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"2 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148957886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanically Programmable Whispering Gallery Mode Microlasers for Information Encryption","authors":"Zhihan Cai,Zhonghao Li,Yuliang Liu,Guifeng Li,Yuan Gong,Xi Yang,Chaoyang Gong,Tao Zhu","doi":"10.1039/d6lc00295a","DOIUrl":"https://doi.org/10.1039/d6lc00295a","url":null,"abstract":"Microlasers featuring multiple emission degrees of freedom offer a unique physical platform for information encryption. However, conventional microlasers provide limited access to the intrinsic mechanical properties of microcavities, leaving this mechanical dimension largely untapped for information encryption. Here, we propose the mechanically programmable whispering gallery mode (WGM) microlasers to address this limitation. The microlasers were fabricated by inkjet printing a dye-doped solution on a superhydrophobic surface. The mechanical properties of the liquid microcavities can be programmed by changing the glycerol concentration. Ultrasound was applied to stimulate the microlaser, and the resulting dynamic laser spectra were used to read out the mechanical properties encoded in the microcavity. We found that the mechanical properties of microlasers establish a physically secure dimension orthogonal to conventional optical methods. We further employed these mechanically programmable WGM microlasers for information encryption. We envision that hybrid optical-mechanical encryption can be achieved through integration with conventional optical methods.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"5 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148915228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of lipid nanoparticle components and microfluidic parameters for intramuscular circRNA delivery with reduced hepatic off-targeting","authors":"Jie Yang,Yijia Hao,Siying Long,Youkui Huang,Gong Wang,Chenxing Yan,Jingxuan Piao,Bowen Wu,Guangyan Huang,Xuexiang Han","doi":"10.1039/d6lc00536e","DOIUrl":"https://doi.org/10.1039/d6lc00536e","url":null,"abstract":"Circular RNA (circRNA) represents a promising class of RNAs for sustained protein expression and vaccine applications. However, current lipid nanoparticle (LNP) platforms remain poorly optimized for efficient intramuscular circRNA delivery, as they are primarily designed for the delivery of small interfering RNA and linear messenger RNA with strong off-target expression in the liver. In this study, based on the clinical benchmark SM-102 LNP, we systematically optimized lipid components and microfluidic parameters for the better intramuscular circRNA delivery. Through in vitro and in vivo screening of a variety of phospholipids and sterols, we identified a 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE)- and cholesterol-based formulation, which demonstrated the improved storage stability and enhanced intramuscular transfection while minimizing hepatic off-target expression. Using ovalbumin as a model antigen, we proved that this formulation-based vaccine elicited more balanced Th1/Th2 immune responses with reduced liver toxicity. Together, this study successfully develops an optimized formulation with reduced hepatic off-target effects, which holds great promise for the intramuscular delivery of circRNA-based vaccines and therapeutics.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"79 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148908817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leaching Behaviour of Stereolithography 3D-printed Microfluidics","authors":"Volkan Cirik,Lívia Kanizsová,Olga Kockova,Nicholas Lynn","doi":"10.1039/d6lc00336b","DOIUrl":"https://doi.org/10.1039/d6lc00336b","url":null,"abstract":"Stereolithography (SLA) 3D printing has become a standard tool to produce microfluidic structures, thanks to advancements in both 3D printer technology and resin materials. Used across many applications, SLA-printed microfluidics are often exposed to a variety of solvents, giving rise to potential leaching from the printed structure (in the form of unreacted resin components) that can potentially interfere with device functionality. In this study, we investigated the leaching behavior of SLA-printed structures from both external surfaces (under static conditions) and microfluidic surfaces (under flowing conditions). We focused on how leaching is affected by factors such as resin and solvent type, postprocessing, fluidic architecture, and internal flow rates. Using a combination of Gas Chromatography-Mass Spectrometry (GC-MS) and High-Performance Liquid Chromatography (HPLC) analysis, we identified 15 chemical species consistently found in leachate samples from 4 commercial resins, in the form of monomers and photoinitiators. We found that increased post-processing led to an overall reduction in leaching, with monomers being more affected than photoinitiators. In no cases was leaching eliminated. For leaching from microfluidic channels, we found that light treatment did not result in any reduction in leachate levels, which can be attributed to the absorption of light in the external regions of the structure. These findings highlight the importance of thorough post-processing, careful solvent selection, and optimal fluid flow conditions to improve the chemical stability and biocompatibility of SLA-printed devices in fluidic applications.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"39 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148908694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetic bead induced surface tension change on gas-solid-liquid interface to manipulate the reciprocating flow of droplet for rapid immunoassay of viruses","authors":"Yiren Liu,Hongrui Liang,Xiyuan Huang,Juanhua Li,Haonan Li,Tianyu Wu,Zihan Xiao,Yibo Wang,Mingxia Sun,Jianhua Zhou","doi":"10.1039/d6lc00654j","DOIUrl":"https://doi.org/10.1039/d6lc00654j","url":null,"abstract":"Droplet manipulation provides tools for medical diagnostics, cell culturing, and drug screening. Among droplet manipulation techniques, magnetic manipulation can transport, mix and separate droplets, garnering broad attention for rapid immunoassay. Typically, magnetic manipulation requires ferrofluid or micron-sized magnetic particles as droplet actuators.However, ferrofluid may contaminate droplet, and magnetic particles may trap unbound molecules (e.g., viruses, antigens) within their aggregation gaps. These disadvantages decrease the accuracy of immunoassay. Here, we use a millimeter-sized magnetic bead to manipulate a droplet by inducing surface tension change on gas-solid-liquid interface. The manipulation of each droplet requires single magnetic bead that releases no solvent or contaminant, eliminating the trapping of unbound molecules in the gaps between aggregated magnetic beads. We further design a chip and its complementary miniaturized analyzer to manipulate the reciprocating flow of droplets, and develop the rapid immunoassay method to detect Sendai virus and pneumonia virus of mice within 10 min. This method can detect target viruses in serum with a sensitivity of 100% (6/6) and a specificity of 93.3% (28/30). This work demonstrates great performance in the droplet manipulation and the rapid immunoassay of viruses, with extensive prospects in medical diagnosis, cell culturing and drug screening.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"15 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148908693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SOFT X-RAY TRANSPARENT FLOW CELL FOR WATER WINDOW MICROSCOPY WITH INTEGRATED DIELECTROPHORETIC CELL HANDLING","authors":"Simone de Carli,Julius Reinhard,Sophia Kaleta,Wilhelm Eschen,Chang Liu,Christian Guernth-Marschner,Marine Vallet,Julie A. Z. A.Z. Zedler,Leona Licht,Gerhard G. Paulus,Silvio Fuchs,Neus Godino,Jan Rothhardt,Michael Kirschbaum","doi":"10.1039/d6lc00276e","DOIUrl":"https://doi.org/10.1039/d6lc00276e","url":null,"abstract":"Soft X-ray (SXR) microscopy in the water window (284 eV – 543 eV) is a powerful technique enabling label-free whole-cell imaging with an intrinsic structural contrast. Studying living cells in their native aqueous environment, rather than cryopreserved specimens, allows direct, time-resolved observation of biological processes and yields physiologically relevant insights. However, imaging hydrated biological specimens under the vacuum conditions required for SXR microscopy remains challenging, requiring sample environments that simultaneously provide liquid confinement, vacuum compatibility, stable cell positioning, and efficient sample handling. Existing SXR-transparent flow cells often rely on artificial adhesion or fixation, limiting active manipulation, and efficient turnover. Here, we present a vacuum-compatible microfluidic flow cell for water-window SXR microscopy with integrated dielectrophoretic (DEP) cell and particle handling. The device combines X-ray-transparent silicon nitride (Si₃N₄) membranes, microfluidic sample exchange, and DEP-based trapping of suspended cells within a thin liquid layer. Unlike conventional static sample holders, the platform allows fluidic replacement of samples without venting the microscope and enables controlled handling of individual suspended cells in their native aqueous environment. Using polystyrene particles and Jurkat cells, we characterized the DEP trapping performance and demonstrate stable confinement within the microfluidic channel. Integrated with a laboratory-based water-window SXR microscope, the platform enables imaging of dielectrophoretically trapped algal cells from different species in liquid during a single days-long vacuum session. These results establish the feasibility of combining DEP-based cell handling, microfluidic sample exchange, and water-window SXR microscopy in a single experimental platform and provide a foundation for future studies requiring controlled manipulation of hydrated cells during SXR imaging.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"61 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148902503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A thread-based microfluidic electrochemical platform for whole-blood protein sensing using endogenous uric acid as a redox mediator","authors":"Toru Nohgi,Sirawit Sornrak,Jixin Shang,Huizi Zhang,Ling Ding,Hiroshi Kawahira,Jun Kameoka","doi":"10.1039/d6lc00533k","DOIUrl":"https://doi.org/10.1039/d6lc00533k","url":null,"abstract":"Electrochemical protein sensing in whole blood remains challenging because many existing assays rely on external redox probes, washing steps, and off-chip pretreatment such as plasma separation. Here, we present a thread-based microfluidic electrochemical platform for protein-related sensing in whole blood without external redox reagents by exploiting endogenous uric acid (UA) as a redox mediator. The device integrates capillary-driven plasma separation and electrochemical detection in a compact format. Within the thread network, blood cells are retained through a Ca2+-induced aggregation mechanism, enabling plasma delivery to the electrode interface without centrifugation. Target recognition is established through a blocking-type mechanism in which antigen–antibody complex formation suppresses the oxidation current of UA. Using C-reactive protein (CRP) as a model target, the platform showed concentration-dependent responses over a clinically relevant range. The limit of detection was 0.091 mg dL−1 in PBS and 0.106 mg dL−1 in the integrated blood-based device configuration. In a stepwise CRP spike-and-recovery experiment, recoveries of 103.9%, 99.6%, and 99.8% were obtained for three concentration levels, with all relative standard deviation (RSD) values below 1.20%. These results demonstrate excellent analytical accuracy and reproducibility of the proposed sensing platform. The inhibition-based signal was preserved across the physiologically relevant range of uric acid concentrations tested, suggesting that the sensing response is relatively insensitive to moderate variations in endogenous uric acid levels. In a preliminary porcine inflammation model, the CRP-related sensor response showed a delayed temporal association with WBC count, with a maximum cross-correlation of R = 0.85 at a lag of approximately 180 min. Together, these results validate the feasibility of reagent-free protein sensing directly from whole blood using endogenous electroactive molecules. This study introduces a simple sample-to-answer thread-based microfluidic platform and highlights a new strategy for decentralized biochemical analysis without external redox mediators.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"213 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148902502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}