Lab on a Chip最新文献_第8页

Reusable EWOD-based microfluidic system for active droplet generation† 基于ewod的可重复使用的主动液滴生成微流控系统。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-12-10 DOI: 10.1039/D4LC00744A

Suhee Park, Jaewook Ryu and Ki-Ho Han

{"title":"Reusable EWOD-based microfluidic system for active droplet generation†","authors":"Suhee Park, Jaewook Ryu and Ki-Ho Han","doi":"10.1039/D4LC00744A","DOIUrl":"10.1039/D4LC00744A","url":null,"abstract":"Droplets are essential in a wide range of microfluidic applications, but traditional passive droplet generation methods suffer from slow response speed and the need for precise flow rate adjustment. Here, we present an active droplet generation method through electrowetting-on-dielectric (EWOD). Electrowetting is a technique that uses an electric field to change the wettability of a surface. In our method, we apply an electric field to the laminar flow of the dispersed and continuous phases in a microchannel, which induces the discretization of the dispersed thread and leads to droplet formation. A key feature of the proposed active droplet-generating microfluidic device is the reusability of the EWOD actuation substrate, dramatically reducing operational costs. In addition, this approach offers significant advantages over passive methods, including fast response speeds, a wider range of droplet sizes, and greater control over droplet size. In addition, the ultrathin polymer film used in this device allows for a low electrowetting voltage, which helps to prevent damage to encapsulated cells. We believe that our active droplet generation method is a promising new method for generating droplets in microfluidic applications. It is faster, more versatile, and more precise than passive methods, making it ideal for a wide range of applications, including single-cell genomics and drug discovery.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 2","pages":" 225-234"},"PeriodicalIF":6.1,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-throughput microfluidic spheroid technology for early detection of colistin-induced nephrotoxicity with gradient-based analysis† 基于梯度分析的高通量微流控球体技术早期检测粘菌素引起的肾毒性。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-12-10 DOI: 10.1039/D4LC00782D

Yugyeong Lee, Yunsang Choi, Ju Lan Chun, Hong Bin Kim, Sejoong Kim, Eu Suk Kim and Sungsu Park

{"title":"High-throughput microfluidic spheroid technology for early detection of colistin-induced nephrotoxicity with gradient-based analysis†","authors":"Yugyeong Lee, Yunsang Choi, Ju Lan Chun, Hong Bin Kim, Sejoong Kim, Eu Suk Kim and Sungsu Park","doi":"10.1039/D4LC00782D","DOIUrl":"10.1039/D4LC00782D","url":null,"abstract":"Colistin is essential for treating multidrug-resistant Gram-negative bacterial infections but has significant nephrotoxic side effects. Traditional approaches for studying colistin's nephrotoxicity are challenged by the rapid metabolism of its prodrug, colistin methanesulfonate and the difficulty of obtaining adequate plasma from critically ill patients. To address these challenges, we developed the Spheroid Nephrotoxicity Assessing Platform (SNAP), a microfluidic device that efficiently detects colistin-induced toxicity in renal proximal tubular epithelial cell (RPTEC) spheroids within 48 hours using just 200 μL of patient plasma. Our findings demonstrate that SNAP not only promotes higher expression of kidney-specific markers aquaporin-1 (AQP1) and low-density lipoprotein receptor-related protein 2 (LRP2) compared to traditional two-dimensional (2D) cultures, but also exhibits increased sensitivity to colistin, with significant toxicity detected at concentrations of 50 μg ml−1 and above. Notably, SNAP's non-invasive method did not identify nephrotoxicity in plasma from healthy donors, thereby confirming its physiological relevance and showcasing superior sensitivity over 2D cultures, which yielded false-positive results. In clinical validation, SNAP accurately identified patients at risk of colistin-induced nephrotoxicity with 100% accuracy for both early and late onset and demonstrated a 75% accuracy rate in predicting the non-occurrence of nephrotoxicity. These results underline the potential of SNAP in personalized medicine, offering a non-invasive, precise and efficient tool for the assessment of antibiotic-induced nephrotoxicity, thus enhancing the safety and efficacy of treatments against resistant bacterial infections.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 2","pages":" 275-284"},"PeriodicalIF":6.1,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Distal renal tubular system-on-a-chip for studying the pathogenesis of influenza A virus-induced kidney injury 更正：远端肾小管系统芯片用于研究甲型流感病毒引起的肾损伤的发病机制。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-12-10 DOI: 10.1039/D4LC90105C

Yueyue Huangfu, Ji Wang, Jiao Feng and Zhi-Ling Zhang

引用次数: 0

A gravity-driven microfluidic metering system for automation of multiplexed bioassays† 用于多路生物检测自动化的重力驱动微流控计量系统。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-12-05 DOI: 10.1039/D4LC00800F

Lu Zhang, Johnson Q. Cui and Shuhuai Yao

{"title":"A gravity-driven microfluidic metering system for automation of multiplexed bioassays†","authors":"Lu Zhang, Johnson Q. Cui and Shuhuai Yao","doi":"10.1039/D4LC00800F","DOIUrl":"10.1039/D4LC00800F","url":null,"abstract":"Automatic and precise fluid manipulation is essential in microfluidic applications. Microfluidic metering, in particular, plays a critical role in achieving the multiplexity of assays, reaction consistency, quantitative analysis, and the scalability of microfluidic operations. However, existing fluid metering techniques often face limitations, such as high complexity, high cost, reliance on external accessories, and lack of precision, which have restricted their use in multiplexed and quantitative analysis, especially in portable applications. In this study, we present a novel portable gravity-driven metering system designed for automated multiplexed fluid metering, multistep fluid control, and multi-chamber signal readout. Our metering chip utilizes gravitational force to dispense sample liquids, allowing for versatile and precise metering. Guided by a series of numerical simulations, we optimized the design of our metering chip to achieve rapid and accurate liquid metering. Furthermore, thermal control valves were employed to facilitate automated and programmable fluid transfer, eliminating the need for external equipment. To enhance user experience, we developed a smartphone-assisted readout pod for seamless integration with the metering chip. We validated the efficacy of our platform through a proof-of-concept multiplexed analysis of urinary biomarkers, demonstrating high sensitivity, specificity, and absolute quantification capabilities. Our gravity-driven metering system shows significant potential for applications in multiplexed diagnostics, drug screening, and material synthesis, effectively addressing critical needs in fluid manipulation and analysis.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 2","pages":" 175-186"},"PeriodicalIF":6.1,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142798643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design of a magnetically responsive artificial cilia array platform for microsphere transport† 磁响应人工纤毛阵列微球传输平台设计。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-12-04 DOI: 10.1039/D4LC00981A

Yan Qiu, Xinwei Cai, Xin Bian and Guoqing Hu

引用次数: 0

Profiling paracrine interactions between hypoxic and normoxic skeletal muscle tissue in a microphysiological system fabricated from 3D printed components† 在由3D打印组件制造的微生理系统中，分析缺氧和常氧骨骼肌组织之间的旁分泌相互作用。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-12-04 DOI: 10.1039/D4LC00603H

Megan L. Rexius-Hall, Malinda D. Madrigal, Cem Y. Kilic, Keyue Shen and Megan L. McCain

{"title":"Profiling paracrine interactions between hypoxic and normoxic skeletal muscle tissue in a microphysiological system fabricated from 3D printed components†","authors":"Megan L. Rexius-Hall, Malinda D. Madrigal, Cem Y. Kilic, Keyue Shen and Megan L. McCain","doi":"10.1039/D4LC00603H","DOIUrl":"10.1039/D4LC00603H","url":null,"abstract":"Disrupted blood flow in conditions such as peripheral artery disease and critical limb ischemia leads to variations in oxygen supply within skeletal muscle tissue, creating regions of poorly perfused, hypoxic skeletal muscle surrounded by regions of adequately perfused, normoxic muscle tissue. These oxygen gradients may have significant implications for muscle injury or disease, as mediated by the exchange of paracrine factors between differentially oxygenated tissue. However, creating and maintaining heterogeneous oxygen landscapes within a controlled experimental setup to ensure continuous paracrine signaling is a technological challenge. Here, we engineer oxygen-controlled microphysiological systems to investigate paracrine interactions between differentially oxygenated engineered muscle tissue. We fabricated microphysiological systems with dual oxygen landscapes that also had engineered control over paracrine interactions between hypoxic and normoxic skeletal muscle tissues, which were differentiated from C2C12 myoblasts cultured on micromolded gelatin hydrogels. The microphysiological systems interfaced with a new 3D-printed oxygen control well plate insert, which we designed to distribute flow to multiple microphysiological systems and minimize evaporation for longer timepoints. With our system, we demonstrated that amphiregulin, a myokine associated with skeletal muscle injury, exhibits unique upregulation in both gene expression and secretion after 24 hours due to paracrine interactions between hypoxic and normoxic skeletal muscle tissue. Our platform can be extended to investigate other impacts of paracrine interactions between hypoxic and normoxic skeletal muscle and can more broadly be used to elucidate many forms of oxygen-dependent crosstalk in other organ systems.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 2","pages":" 212-224"},"PeriodicalIF":6.1,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An integrated microfluidic device for sorting of tumor organoids using image recognition† 一种基于图像识别的肿瘤类器官分选集成微流控装置。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-12-04 DOI: 10.1039/D4LC00746H

Xingyang Yan, Deng Tan, Lei Yu, Danyu Li, Zhenghao Wang, Weiren Huang and Hongkai Wu

引用次数: 0

Rapid automated production of tubular 3D intestine-on-a-chip with diverse cell types using coaxial bioprinting† 使用同轴生物打印快速自动化生产具有不同细胞类型的管状3D肠道芯片。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-12-03 DOI: 10.1039/D4LC00731J

Heeju Song, Yeonjin Hong and Hyungseok Lee

{"title":"Rapid automated production of tubular 3D intestine-on-a-chip with diverse cell types using coaxial bioprinting†","authors":"Heeju Song, Yeonjin Hong and Hyungseok Lee","doi":"10.1039/D4LC00731J","DOIUrl":"10.1039/D4LC00731J","url":null,"abstract":"Despite considerable animal sacrifices and investments, drug development often falters in clinical trials due to species differences. To address this issue, specific in vitro models, such as organ-on-a-chip technology using human cells in microfluidic devices, are recognized as promising alternatives. Among the various organs, the human small intestine plays a pivotal role in drug development, particularly in the assessment of digestion and nutrient absorption. However, current intestine-on-a-chip devices struggle to accurately replicate the complex 3D tubular structures of the human small intestine, particularly when it comes to integrating a variety of cell types effectively. This limitation is primarily due to conventional fabrication methods, such as soft lithography and replica molding. In this research, we introduce a novel coaxial bioprinting method to construct 3D tubular structures that closely emulate the organization and functionality of the small intestine with multiple cell types. To ensure stable production of these small intestine-like tubular structures, we analyzed the rheological properties of bioinks to select the most suitable materials for coaxial bioprinting technology. Additionally, we conducted biological assessments to validate the gene expression patterns and functional attributes of the 3D intestine-on-a-chip. Our 3D intestine-on-a-chip, which faithfully replicates intestinal functions and organization, demonstrates clear superiority in both structure and biological function compared to the conventional 2D model. This innovative approach holds significant promise for a wide range of future applications.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 1","pages":" 90-101"},"PeriodicalIF":6.1,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142793997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OoTrap: enhancing oocyte collection and maturation with a field-deployable fluidic device† OoTrap：增强卵母细胞收集和成熟与现场可展开的流体装置。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-12-03 DOI: 10.1039/D4LC00660G

Roksan Franko and Marcia de Almeida Monteiro Melo Ferraz

{"title":"OoTrap: enhancing oocyte collection and maturation with a field-deployable fluidic device†","authors":"Roksan Franko and Marcia de Almeida Monteiro Melo Ferraz","doi":"10.1039/D4LC00660G","DOIUrl":"10.1039/D4LC00660G","url":null,"abstract":"Assisted reproductive technologies (ART) are pivotal for contemporary reproductive medicine and species conservation. However, the manual handling required in these processes introduces stress that can compromise oocyte and embryo quality. This study introduces OoTrap, a novel fluidic device designed to streamline ART workflows by facilitating the capture and maturation of oocytes in a compact unit. The device also reintroduces mechanical forces similar to those in the in vivo environment, which are often missing in conventional systems. OoTrap operates in both static and perfusion-based modes, offering flexibility and optimal conditions for oocyte maturation. Notably, OoTrap achieved higher in vitro maturation (IVM) rates under perfusion, produced oocytes with fewer chromosomal abnormalities, and maintained spindle morphology integrity. The incorporation of a heating system and a 3D-printed syringe pump enabled IVM outside the incubator, making OoTrap suitable for field applications. The results highlight the potential of OoTrap to enhance ART outcomes by reducing manual handling, providing a controlled microenvironment, and offering a practical solution for field-based ART applications.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 2","pages":" 187-200"},"PeriodicalIF":6.1,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d4lc00660g?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A 3D-printed multi-compartment organ-on-chip platform with a tubing-free pump models communication with the lymph node† 一个带有无管泵的3d打印多室器官芯片平台模型与淋巴结的通信。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-12-02 DOI: 10.1039/D4LC00489B

Sophie R. Cook, Alexander G. Ball, Anwaruddin Mohammad and Rebecca R. Pompano

{"title":"A 3D-printed multi-compartment organ-on-chip platform with a tubing-free pump models communication with the lymph node†","authors":"Sophie R. Cook, Alexander G. Ball, Anwaruddin Mohammad and Rebecca R. Pompano","doi":"10.1039/D4LC00489B","DOIUrl":"10.1039/D4LC00489B","url":null,"abstract":"Multi-organ-on-chip systems (MOOCs) have the potential to mimic communication between organ systems and reveal mechanisms of health and disease. However, many existing MOOCs are challenging for non-experts to implement due to complex tubing, electronics, or pump mechanisms. In addition, few MOOCs have incorporated immune organs such as the lymph node (LN), limiting their applicability to model critical events such as vaccination. Here we developed a 3D-printed, user-friendly device and companion tubing-free impeller pump with the capacity to co-culture two or more tissue samples, including a LN, under a recirculating common media. Native tissue structure and immune function were incorporated by maintaining slices of murine LN tissue ex vivo in 3D-printed mesh supports for at least 24 h. In a two-compartment model of a LN and an upstream injection site in mock tissue, vaccination of the multi-compartment chip was similar to in vivo vaccination in terms of locations of antigen accumulation and acute changes in activation markers and gene expression in the LN. We anticipate that in the future, this flexible platform will enable models of multi-organ immune responses throughout the body.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 2","pages":" 155-174"},"PeriodicalIF":6.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11633827/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0