Lab on a Chip最新文献_第8页

Dimensional analysis meets AI for non-Newtonian droplet generation†

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-02-12 DOI: 10.1039/D4LC00946K

Farnoosh Hormozinezhad, Claire Barnes, Alexandre Fabregat, Salvatore Cito and Francesco Del Giudice

{"title":"Dimensional analysis meets AI for non-Newtonian droplet generation†","authors":"Farnoosh Hormozinezhad, Claire Barnes, Alexandre Fabregat, Salvatore Cito and Francesco Del Giudice","doi":"10.1039/D4LC00946K","DOIUrl":"10.1039/D4LC00946K","url":null,"abstract":"Non-Newtonian droplets are used across various applications, including pharmaceuticals, food processing, drug delivery and material science. However, predicting droplet formation using such complex fluids is challenging due to the intricate multiphase interactions between fluids with varying viscosities, elastic properties and geometrical constraints. In this study, we introduce a novel hybrid machine-learning architecture that integrates dimensional analysis with machine learning to predict the flow rates required to generate droplets with specified sizes in systems involving non-Newtonian fluids. Unlike previous approaches, our model is designed to accommodate shear-rate-dependent viscosities and a simple estimate of the elastic properties of the fluids. It provides accurate predictions of the dispersed and continuous phases flow rates for given droplet length, height, and viscosity curves, even when the fluid properties deviate from those used during training. Our model demonstrates strong predictive power, achieving R2 values of up to 0.82 for unseen data. The significance of our work lies in its ability to generalize across a broad range of non-Newtonian systems having different viscosity curves, offering a powerful tool for optimizing droplet generation. This model represents a significant advancement in the application of machine learning to microfluidics, providing new opportunities for efficient experimental design in complex multiphase systems.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 7","pages":" 1681-1693"},"PeriodicalIF":6.1,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834948/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soft, wearable, microfluidic system for fluorometric analysis of loss of amino acids through eccrine sweat†

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-02-11 DOI: 10.1039/D4LC00734D

Seunghee H. Cho, Soongwon Cho, Zengyao Lv, Yurina Sekine, Shanliangzi Liu, Mingyu Zhou, Ravi F. Nuxoll, Evangelos E. Kanatzidis, Roozbeh Ghaffari, Donghwan Kim, Yonggang Huang and John A. Rogers

{"title":"Soft, wearable, microfluidic system for fluorometric analysis of loss of amino acids through eccrine sweat†","authors":"Seunghee H. Cho, Soongwon Cho, Zengyao Lv, Yurina Sekine, Shanliangzi Liu, Mingyu Zhou, Ravi F. Nuxoll, Evangelos E. Kanatzidis, Roozbeh Ghaffari, Donghwan Kim, Yonggang Huang and John A. Rogers","doi":"10.1039/D4LC00734D","DOIUrl":"10.1039/D4LC00734D","url":null,"abstract":"Amino acids are essential for protein synthesis and metabolic processes in support of homeostatic balance and healthy body functions. This study quantitatively investigates eccrine sweat as a significant channel for loss of amino acids during exercise, to improve an understanding of amino acid turnover and to provide feedback to users on the need for supplement intake. The measurement platform consists of a soft, skin-interfaced microfluidic system for real-time analysis of amino acid content in eccrine sweat. This system relies on integrated fluorometric assays and smartphone-based imaging techniques for quantitative analysis, as a simple, cost-effective approach that does not require electrochemical sensors, electronics or batteries. Human subject studies reveal substantial amino acid losses in sweat from working muscle regions during prolonged physical activities, thereby motivating the need for dietary supplementation. The findings suggest potential applications in healthcare, particularly in athletic and clinical settings, where maintaining amino acid balance is critical for ensuring proper homeostasis.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 7","pages":" 1647-1655"},"PeriodicalIF":6.1,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smartphones as a platform for molecular analysis: concepts, methods, devices and future potential†

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-02-07 DOI: 10.1039/D4LC00966E

Daina V. Baker, Jasmine Bernal-Escalante, Christine Traaseth, Yihao Wang, Michael V. Tran, Seth Keenan and W. Russ Algar

{"title":"Smartphones as a platform for molecular analysis: concepts, methods, devices and future potential†","authors":"Daina V. Baker, Jasmine Bernal-Escalante, Christine Traaseth, Yihao Wang, Michael V. Tran, Seth Keenan and W. Russ Algar","doi":"10.1039/D4LC00966E","DOIUrl":"10.1039/D4LC00966E","url":null,"abstract":"Over the past 15 years, smartphones have had a transformative effect on everyday life. These devices also have the potential to transform molecular analysis over the next 15 years. The cameras of a smartphone, and its many additional onboard features, support optical detection and other aspects of engineering an analytical device. This article reviews the development of smartphones as platforms for portable chemical and biological analysis. It is equal parts conceptual overview, technical tutorial, critical summary of the state of the art, and outlook on how to advance smartphones as a tool for analysis. It further discusses the motivations for adopting smartphones as a portable platform, summarizes their enabling features and relevant optical detection methods, then highlights complementary technologies and materials such as 3D printing, microfluidics, optoelectronics, microelectronics, and nanoparticles. The broad scope of research and key advances from the past 7 years are reviewed as a prelude to a perspective on the challenges and opportunities for translating smartphone-based lab-on-a-chip devices from prototypes to authentic applications in health, food and water safety, environmental monitoring, and beyond. The convergence of smartphones with smart assays and smart apps powered by machine learning and artificial intelligence holds immense promise for realizing a future for molecular analysis that is powerful, versatile, democratized, and no longer just the stuff of science fiction.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 5","pages":" 884-955"},"PeriodicalIF":6.1,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d4lc00966e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EV-Lev: extracellular vesicle isolation from human plasma using microfluidic magnetic levitation device†

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-02-07 DOI: 10.1039/D4LC00830H

Sena Yaman, Tessa Devoe, Ugur Aygun, Ugur Parlatan, Madhusudhan Reddy Bobbili, Asma H. Karim, Johannes Grillari and Naside Gozde Durmus

{"title":"EV-Lev: extracellular vesicle isolation from human plasma using microfluidic magnetic levitation device†","authors":"Sena Yaman, Tessa Devoe, Ugur Aygun, Ugur Parlatan, Madhusudhan Reddy Bobbili, Asma H. Karim, Johannes Grillari and Naside Gozde Durmus","doi":"10.1039/D4LC00830H","DOIUrl":"10.1039/D4LC00830H","url":null,"abstract":"Biological nanomaterials have unique magnetic and density characteristics that can be employed to isolate them into subpopulations. Extracellular nanovesicles (EVs) are crucial for cellular communication; however, their isolation poses significant challenges due to their diverse sizes and compositions. We present EV-Lev, a microfluidic magnetic levitation technique for high-throughput, selective isolation of small EVs (<200 nm) from human plasma. EV-Lev overcomes the challenges posed by the subtle buoyancy characteristics of EVs, whose small size and varied densities complicate traditional magnetic levitation techniques. It employs antibody-coated polymer beads of varying densities, integrating immuno-affinity and microfluidics to isolate EVs from sub-milliliter plasma volumes efficiently. It facilitates rapid, simultaneous sorting of EV subpopulations based on surface markers, such as CD9, CD63, and CD81, achieving high yield and purity. Subsequent size and morphology analyses confirmed that the isolated EVs maintain their structural integrity. EV-Lev could help uncover the cargo and function of EV subpopulations associated with multiple diseases including cancer, infectious diseases and help to discover potential biomarkers in small volume samples, while offering a portable, cost-effective, and straightforward assay scheme.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 6","pages":" 1439-1451"},"PeriodicalIF":6.1,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic paper-based analytical soft actuators (μPAC).

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-02-06 DOI: 10.1039/d4lc00602j

Koki Yoshida, Masahiro Tanakinoue, Hiroaki Onoe, Michinao Hashimoto

{"title":"Microfluidic paper-based analytical soft actuators (μPAC).","authors":"Koki Yoshida, Masahiro Tanakinoue, Hiroaki Onoe, Michinao Hashimoto","doi":"10.1039/d4lc00602j","DOIUrl":"https://doi.org/10.1039/d4lc00602j","url":null,"abstract":"Soft actuators have developed over the last decade for diverse applications including industrial machines and biomedical devices. Integration of chemical sensors with soft actuators would be beneficial in analyzing chemical and environmental conditions, but there have been limited devices to achieve such sensing capabilities. In this work, we developed a thin-film soft actuator integrated with a paper-based chemical sensor, termed a microfluidic paper-based analytical soft actuator (μPAC). μPAC consists of (1) a silicone thin film with a 3D-printed pneumatic chamber and (2) a cellulose paper. This cellulose paper offers dual functions: the strain-limiting layer of a soft actuator and the substrate for the chemical sensor for a paper-based analytical device (μPAD). We characterized the design parameters of the actuators-namely, (1) thickness of silicone thin film, (2) chamber length, and (3) Young's modulus of silicone thin film-to evaluate the actuation performance. These characterizations suggested that the cellulose paper served as a suitable self-straining layer of the actuator, making μPAC a chemical sensor that can actuate simultaneously. Highlighting the unique capability of μPAC, we demonstrated the local detection of pH on the curved target surface. Overall, this research demonstrated the rapid fabrication of actuating chemical sensors with a unique design by combining soft actuators and μPAD, enabling chemical sensing on various surface topologies by dynamically making conformal contact.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143254396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A plug-and-play microfluidic device for hydrogel fiber spinning†

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-02-06 DOI: 10.1039/D4LC00783B

Kongchang Wei, Wuchao Wang, Giorgia Giovannini, Khushdeep Sharma, René M. Rossi and Luciano F. Boesel

{"title":"A plug-and-play microfluidic device for hydrogel fiber spinning†","authors":"Kongchang Wei, Wuchao Wang, Giorgia Giovannini, Khushdeep Sharma, René M. Rossi and Luciano F. Boesel","doi":"10.1039/D4LC00783B","DOIUrl":"10.1039/D4LC00783B","url":null,"abstract":"Hydrogel fibers are promising biomaterials for a broad range of biomedical applications, including biosensing, drug delivery, and tissue engineering. Different types of microfluidic devices have been developed for hydrogel fiber spinning, however, they often require skillful fabrication procedures with special instruments such as 3D printers and clean-room facilities. On the other hand, microfluidic devices with predetermined and fixed configurations are susceptible to clotting, contamination, and damage, thereby creating a significant barrier for potential users. Herein, we describe a plug-and-play (PnP) microfluidic device for hydrogel fiber spinning. The PnP device was designed to be assembled in a modular manner based on simple mounting of PDMS elastomers on commercial Lego® blocks. Easy disassembly and re-assembly make the device user-friendly, since cleaning or replacing individual modules is convenient. We demonstrated the application of our PnP microfluidic device in alginate (Alg) hydrogel fiber spinning by using a single-module or double-module device. Moreover, thanks to the PnP approach, multi-layered fibers can be produced by using a triple-module device. As proof-of-principle, we fabricated pH-sensitive multi-layered fibers that could be used for monitoring biological environments, showcasing the potential of such a PnP device in advancing biomedical research related to functional fibers.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 6","pages":" 1575-1585"},"PeriodicalIF":6.1,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11815318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143397622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and simulation of biomimetic microfluidic designs to achieve uniform flow and DNA capture for high-throughput multiplexing†

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-02-05 DOI: 10.1039/D4LC01023J

Enas Osman, Jonathan L'Heureux-Hache, Phoebe Li and Leyla Soleymani

{"title":"Design and simulation of biomimetic microfluidic designs to achieve uniform flow and DNA capture for high-throughput multiplexing†","authors":"Enas Osman, Jonathan L'Heureux-Hache, Phoebe Li and Leyla Soleymani","doi":"10.1039/D4LC01023J","DOIUrl":"10.1039/D4LC01023J","url":null,"abstract":"High-throughput multi-analyte point-of-care detection is often constrained by the limited number of analytes that can be effectively monitored. This study introduces bio-inspired microfluidic designs optimized for multi-analyte detection using 38–42 biosensors. Drawing inspiration from the human spinal cord and leaf vein networks, these perfusion-oriented designs ensure uniform flow velocity and consistent molecular capture while maintaining spatial separation to prevent cross-talk. In silico optimizations achieved velocity profile uniformity with coefficients of variance of 0.89% and 0.86% for the spine- and leaf-inspired designs, respectively. However, simulations revealed that velocity uniformity alone is insufficient for accurate molecular capture prediction without consistent reaction site channel dimensions. The bio-inspired designs demonstrated superior performance, stabilizing—coefficient of variance below 20%—in DNA capture within 10 minutes, compared to 68 minutes for a simple branched design. This work underscores the potential of bio-inspired microfluidics to enable scalable, uniform, and high-performance systems for multi-analyte detection.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 6","pages":" 1462-1473"},"PeriodicalIF":6.1,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d4lc01023j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143397623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TapeTech microfluidic connectors: adhesive tape-enabled solution for organ-on-a-chip system integration†

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-02-05 DOI: 10.1039/D4LC00970C

Terry Ching, Abraham C. I. van Steen, Delaney Gray-Scherr, Jessica L. Teo, Anish Vasan, Joshua Jeon, Jessica Shah, Aayush Patel, Amy E. Stoddard, Jennifer L. Bays, Jeroen Eyckmans and Christopher S. Chen

{"title":"TapeTech microfluidic connectors: adhesive tape-enabled solution for organ-on-a-chip system integration†","authors":"Terry Ching, Abraham C. I. van Steen, Delaney Gray-Scherr, Jessica L. Teo, Anish Vasan, Joshua Jeon, Jessica Shah, Aayush Patel, Amy E. Stoddard, Jennifer L. Bays, Jeroen Eyckmans and Christopher S. Chen","doi":"10.1039/D4LC00970C","DOIUrl":"10.1039/D4LC00970C","url":null,"abstract":"A longstanding challenge in microfluidics has been the efficient delivery of fluids from macro-scale pumping systems into microfluidic devices, known as the “world-to-chip” problem. Thus far, the entire industry has accepted the use of imperfect, rigid tubing and connectors as the ecosystem within which to operate, which, while functional, are often cumbersome, labor-intensive, prone to errors, and ill-suited for high-throughput experimentation. In this paper, we introduce TapeTech microfluidics, a flexible and scalable solution designed to address the persistent “world-to-chip” problem in microfluidics, particularly in organ-on-a-chip (OoC) applications. TapeTech offers a streamlined alternative, utilizing adhesive tape and thin-film polymers to create adaptable, integrated multi-channel ribbon connectors that simplify fluidic integration with pumps and reservoirs. Key features of TapeTech include reduced pressure surges, easy priming, rapid setup, easy multiplexing, and broad compatibility with existing devices and components, which are essential for maintaining stable fluid dynamics and protecting sensitive cell cultures. Furthermore, TapeTech is designed to flex around the lids of Petri dishes, enhancing sterility and transportability by enabling easy transfer between incubators, biosafety cabinets (BSCs), and microscopes. The rapid design-to-prototype iteration enabled by TapeTech allows users to quickly develop connectors for a wide range of microfluidic devices. Importantly, we showcase the utility of TapeTech in OoC cultures requiring fluid flow. We also highlight other utilities, such as real-time microscopy and a well-plate medium exchanger. The accessibility of this technology should enable more laboratories to simplify design and setup of microfluidic experiments, and increase technology adoption.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 6","pages":" 1474-1488"},"PeriodicalIF":6.1,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11795533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143187800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A sample-to-answer digital microfluidic multiplexed PCR system for syndromic pathogen detection in respiratory tract infection†

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-02-05 DOI: 10.1039/D4LC00704B

Hao Bai, Jie Hu, Tangyuheng Liu, Liang Wan, Cheng Dong, Dasheng Luo, Fei Li, Zhanxin Yuan, Yunmei Tang, Tianlan Chen, Shan Wang, Hongna Gou, Yongzhao Zhou, Binwu Ying, Jin Huang and Wenchuang (Walter) Hu

{"title":"A sample-to-answer digital microfluidic multiplexed PCR system for syndromic pathogen detection in respiratory tract infection†","authors":"Hao Bai, Jie Hu, Tangyuheng Liu, Liang Wan, Cheng Dong, Dasheng Luo, Fei Li, Zhanxin Yuan, Yunmei Tang, Tianlan Chen, Shan Wang, Hongna Gou, Yongzhao Zhou, Binwu Ying, Jin Huang and Wenchuang (Walter) Hu","doi":"10.1039/D4LC00704B","DOIUrl":"10.1039/D4LC00704B","url":null,"abstract":"Timely identification of infectious pathogens is crucial for the accurate diagnosis, management, and treatment of syndromic respiratory diseases. Nevertheless, the implementation of rapid, precise, and automated point-of-care testing (POCT) remains a significant challenge. This study introduces an advanced digital microfluidic (DMF) POCT testing system designed for the rapid molecular syndromic testing of multiple respiratory pathogens from a single untreated sample. The system seamlessly integrates magnetic beads-based nucleic acid extraction, PCR amplification, and real-time fluorescence analysis in an automatic run, facilitating sample-to-answer detection within 80 min. It accommodates various sample types, including nasopharyngeal swabs, oropharyngeal swabs, bronchoalveolar lavage fluid, and sputum. A facile sample loading method has been developed to reduce hands-on time to less than 1 min. The system exhibits high sensitivity (200–628 copies per mL) for 15 pathogens and has the capacity for up to 32 multiplexed tests per run. Validation with 255 clinical samples confirms its high sensitivity and specificity. The DMF-based system significantly reduces manual labour, enhances rapid POCT for respiratory infections, and, with optimized manufacturing processes, lowers costs for large-scale production. The system can be applied and improve clinical management near the patients as well as in resource-limited settings.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 6","pages":" 1552-1564"},"PeriodicalIF":6.1,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143187795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bone microphysiological models for biomedical research

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-02-05 DOI: 10.1039/D4LC00762J

Francisco Verdugo-Avello, Jacek K. Wychowaniec, Carlos A. Villacis-Aguirre, Matteo D'Este and Jorge R. Toledo

{"title":"Bone microphysiological models for biomedical research","authors":"Francisco Verdugo-Avello, Jacek K. Wychowaniec, Carlos A. Villacis-Aguirre, Matteo D'Este and Jorge R. Toledo","doi":"10.1039/D4LC00762J","DOIUrl":"10.1039/D4LC00762J","url":null,"abstract":"Bone related disorders are highly prevalent, and many of these pathologies still do not have curative and definitive treatment methods. This is due to a complex interplay of multiple factors, such as the crosstalk between different tissues and cellular components, all of which are affected by microenvironmental factors. Moreover, these bone pathologies are specific, and current treatment results vary from patient to patient owing to their intrinsic biological variability. Current approaches in drug development to deliver new drug candidates against common bone disorders, such as standard two-dimensional (2D) cell culture and animal-based studies, are now being replaced by more relevant diseases modelling, such as three-dimension (3D) cell culture and primary cells under human-focused microphysiological systems (MPS) that can resemble human physiology by mimicking 3D tissue organization and cell microenvironmental cues. In this review, various technological advancements for in vitro bone modeling are discussed, highlighting the progress in biomaterials used as extracellular matrices, stem cell biology, and primary cell culture techniques. With emphasis on examples of modeling healthy and disease-associated bone tissues, this tutorial review aims to survey current approaches of up-to-date bone-on-chips through MPS technology, with special emphasis on the scaffold and chip capabilities for mimicking the bone extracellular matrix as this is the key environment generated for cell crosstalk and interaction. The relevant bone models are studied with critical analysis of the methods employed, aiming to serve as a tool for designing new and translational approaches. Additionally, the features reported in these state-of-the-art studies will be useful for modeling bone pathophysiology, guiding future improvements in personalized bone models that can accelerate drug discovery and clinical translation.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 5","pages":" 806-836"},"PeriodicalIF":6.1,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d4lc00762j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143187796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0