Mechanical actuation on surface (MAOS) microfluidics: compression for preparation in next-generation sequencing.

IF 5.4 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS
Lab on a Chip Pub Date : 2025-08-29 DOI:10.1039/d5lc00625b
Parimala Nagaraja, Rohit Lal, Cheng-Chang Lee, Eduardo Cervantes, Foteini Christodoulou, Mais J Jebrail
{"title":"Mechanical actuation on surface (MAOS) microfluidics: compression for preparation in next-generation sequencing.","authors":"Parimala Nagaraja, Rohit Lal, Cheng-Chang Lee, Eduardo Cervantes, Foteini Christodoulou, Mais J Jebrail","doi":"10.1039/d5lc00625b","DOIUrl":null,"url":null,"abstract":"<p><p>We present mechanical actuation on surface (MAOS), a programmable microfluidic platform that manipulates droplets <i>via</i> localized mechanical compression-eliminating the need for embedded electronics or fixed microchannel geometries. MAOS integrates essential fluidic operations-including droplet transport, magnetic bead-based purification, and thermal cycling-within a benchtop instrument and single-use cartridge. The system accommodates droplet volumes from nL to μL, enabling precise control over sequential biochemical processes. By studying the dynamic behavior of diverse fluids under compression, we identified the key physical variables-surface tension, contact angle, and viscosity-that dictate the onset of droplet motion. We observed sharp transitions in mobility around specific thresholds and validated interfacial encapsulation as a general strategy to overcome resistive pinning. We validated MAOS by first implementing and testing miniaturized next-generation sequencing (NGS) library preparation sub-processes. Magnetic bead-based cleanup showed DNA recovery and fragment size selection comparable to manual methods, and PCR amplification was carried out reliably in low-volume (5 μL) reactions with minimal evaporation. Subsequently, the full NGS library preparation workflow was executed in a plexed format, processing eight libraries in parallel on a single disposable cartridge using as little as 10% of standard reagent volumes. Short- and long-read sequencing outputs from MAOS libraries aligned with manual protocols across key quality metrics. These results establish MAOS as a scalable and user-friendly alternative to conventional microfluidics, suitable for diverse applications in molecular biology, chemistry, and high-throughput workflows.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":5.4000,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lab on a Chip","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1039/d5lc00625b","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

Abstract

We present mechanical actuation on surface (MAOS), a programmable microfluidic platform that manipulates droplets via localized mechanical compression-eliminating the need for embedded electronics or fixed microchannel geometries. MAOS integrates essential fluidic operations-including droplet transport, magnetic bead-based purification, and thermal cycling-within a benchtop instrument and single-use cartridge. The system accommodates droplet volumes from nL to μL, enabling precise control over sequential biochemical processes. By studying the dynamic behavior of diverse fluids under compression, we identified the key physical variables-surface tension, contact angle, and viscosity-that dictate the onset of droplet motion. We observed sharp transitions in mobility around specific thresholds and validated interfacial encapsulation as a general strategy to overcome resistive pinning. We validated MAOS by first implementing and testing miniaturized next-generation sequencing (NGS) library preparation sub-processes. Magnetic bead-based cleanup showed DNA recovery and fragment size selection comparable to manual methods, and PCR amplification was carried out reliably in low-volume (5 μL) reactions with minimal evaporation. Subsequently, the full NGS library preparation workflow was executed in a plexed format, processing eight libraries in parallel on a single disposable cartridge using as little as 10% of standard reagent volumes. Short- and long-read sequencing outputs from MAOS libraries aligned with manual protocols across key quality metrics. These results establish MAOS as a scalable and user-friendly alternative to conventional microfluidics, suitable for diverse applications in molecular biology, chemistry, and high-throughput workflows.

表面机械驱动(MAOS)微流体:用于下一代测序制备的压缩。
我们提出了表面机械驱动(MAOS),这是一种可编程的微流体平台,通过局部机械压缩来操纵液滴,从而消除了对嵌入式电子设备或固定微通道几何形状的需求。MAOS集成了基本的流体操作,包括液滴传输,基于磁珠的净化和热循环,在台式仪器和一次性使用的墨盒内。该系统可容纳从nL到μL的液滴体积,能够精确控制连续的生化过程。通过研究不同流体在压缩下的动态行为,我们确定了决定液滴运动开始的关键物理变量——表面张力、接触角和粘度。我们观察到迁移率在特定阈值附近的急剧转变,并验证了界面封装作为克服电阻钉钉的一般策略。我们首先通过实施和测试小型化的下一代测序(NGS)文库制备子流程来验证MAOS。磁珠清理显示DNA恢复和片段大小选择与手工方法相当,并且在低体积(5 μL)反应中可靠地进行PCR扩增,蒸发最小。随后,以混合格式执行完整的NGS文库制备工作流程,在单个一次性墨盒上并行处理8个文库,使用的标准试剂体积仅为10%。来自MAOS库的短读和长读测序输出与跨关键质量指标的手动协议一致。这些结果建立了MAOS作为传统微流体的可扩展和用户友好的替代品,适用于分子生物学,化学和高通量工作流程中的各种应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Lab on a Chip
Lab on a Chip 工程技术-化学综合
CiteScore
11.10
自引率
8.20%
发文量
434
审稿时长
2.6 months
期刊介绍: Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信