高密度微电极阵列大规模电生理研究进展

IF 5.4 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS
Lab on a Chip Pub Date : 2025-08-28 DOI:10.1039/D5LC00058K
Manuel Schröter, Fernando Cardes, Cat-Vu H. Bui, Lorenzo Davide Dodi, Tobias Gänswein, Julian Bartram, Lorenca Sadiraj, Philipp Hornauer, Sreedhar Kumar, Maria Pascual-Garcia and Andreas Hierlemann
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引用次数: 0

摘要

通过高密度微电极阵列(hd - mea)对神经元和心肌细胞等电致细胞的详细功能表征已成为推断细胞表型和阐明细胞功能基本机制的有力方法。hd - mea已应用于一系列学科,包括神经发育研究、干细胞生物学和药理学,以及最近在生物医学工程、计算机科学和人工智能(AI)交叉领域的跨学科工作。芯片设计、制造、记录能力和数据处理方面的创新大大提高了hd - mea的功能。今天的芯片可以跨尺度和高通量研究细胞功能。它们能够在长时间内分析多参数功能表型,并有助于监测目标扰动对细胞行为的影响。在本教程回顾中,我们将首先调查HD-MEA设计及其读出和刺激能力的进展。然后,我们将总结使用hd - mea与其他实验技术相结合的研究,以探索生物学相关的细胞和亚细胞特征,重点是hd - mea的体外应用。此后,我们将介绍对分析和表征HD-MEA数据至关重要的分析技术。最后,我们将讨论当前hd - mea的局限性,并讨论潜在的未来发展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Advances in large-scale electrophysiology with high-density microelectrode arrays

Advances in large-scale electrophysiology with high-density microelectrode arrays

Advances in large-scale electrophysiology with high-density microelectrode arrays

A detailed functional characterization of electrogenic cells, such as neurons and cardiomyocytes, by means of high-density microelectrode arrays (HD-MEAs) has emerged as a powerful approach for inferring cellular phenotypes and elucidating fundamental mechanisms underlying cellular function. HD-MEAs have been applied across a range of disciplines, including neurodevelopmental research, stem cell biology, and pharmacology, and more recently in interdisciplinary work at the intersection of biomedical engineering, computer science, and artificial intelligence (AI). Innovations in chip design, fabrication, recording capabilities, and data processing have significantly advanced the functionality of HD-MEAs. Today's chips allow the study of cellular function across scales and at high throughput. They enable the analysis of multi-parametric functional phenotypes over extended time and facilitate monitoring the effects of targeted perturbations on cellular behavior. In this Tutorial Review, we will first survey the advances in HD-MEA design and their readout and stimulation capabilities. We will then abstract studies that used HD-MEAs in combination with other experimental techniques to probe biologically relevant cellular and subcellular features, with an emphasis on in vitro applications of HD-MEAs. Thereafter, we will cover analytical techniques that are essential for analyzing and characterizing HD-MEA data. Finally, we will address current limitations of HD-MEAs and discuss potential future developments.

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来源期刊
Lab on a Chip
Lab on a Chip 工程技术-化学综合
CiteScore
11.10
自引率
8.20%
发文量
434
审稿时长
2.6 months
期刊介绍: Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.
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