Lab on a Chip最新文献_第2页

Design advances in pinched flow fractionation for enhanced particle separation in microfluidics 微流体中加强颗粒分离的挤压流分馏的设计进展

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-18 DOI: 10.1039/d5lc00497g

Qi Cui, Guizhong Tian, Tianyuan Zhou, Yigang Shen, Shilun Feng, Ming Li, Weihua Li, Yoichiroh Hosokawa, Tianlong Zhang, Yaxiaer Yalikun

{"title":"Design advances in pinched flow fractionation for enhanced particle separation in microfluidics","authors":"Qi Cui, Guizhong Tian, Tianyuan Zhou, Yigang Shen, Shilun Feng, Ming Li, Weihua Li, Yoichiroh Hosokawa, Tianlong Zhang, Yaxiaer Yalikun","doi":"10.1039/d5lc00497g","DOIUrl":"https://doi.org/10.1039/d5lc00497g","url":null,"abstract":"Pinched flow fractionation (PFF) is a simple, biocompatible microfluidic technique for particle separation, well-suited for applications requiring easy design, ease of operation, and gentle sample handling. In this review, we systematically summarize design advances in PFF, categorizing innovations into five core strategies: broadened segment optimization, pinched segment modification, outlet design refinement, active method integration, and passive hybridization. Both the optimized designs and their underlying working mechanisms are elucidated. While PFF inherently operates as a size-based separation method, these developments have expanded its applicability to shape- and density-based separations. Key performance enhancements are highlighted, e.g., modifications to the pinched and broadened segments increase separation distance, microvalve-integrated outlets enable real-time control, active method integration improves separation resolution, and inertial microfluidic hybridization enhances throughput. Besides, we review representative applications of PFF, like the separation of extracellular vesicles for immunoblotting and microplastics for water quality evaluation. Design guidelines promoting the separation performance are discussed, alongside potential biological particle targets and a comparative analysis of PFF and the other separation techniques. Finally, future directions are proposed, emphasizing the integration of passive methods and device parallelization to maintain PFF’s simplicity while improving throughput and separation capabilities. This review aims to provide theoretical insights and technical guidance for continuous innovation in PFF, promoting its practical implementation across biomedical and environmental monitoring fields.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144652373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Throughput Impedance Monitoring in 3D Tumor Cultures: A Multiplex, Microfluidic-Free Platform for Drug Screening 三维肿瘤培养中的高通量阻抗监测：一个多路、无微流体的药物筛选平台

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-17 DOI: 10.1039/d5lc00540j

Attilio Marino, Kamil Ziaja, Marie Celine Lefevre, Maria Cristina Ceccarelli, Matteo Battaglini, Carlo Filippeschi, Gianni Ciofani

{"title":"High-Throughput Impedance Monitoring in 3D Tumor Cultures: A Multiplex, Microfluidic-Free Platform for Drug Screening","authors":"Attilio Marino, Kamil Ziaja, Marie Celine Lefevre, Maria Cristina Ceccarelli, Matteo Battaglini, Carlo Filippeschi, Gianni Ciofani","doi":"10.1039/d5lc00540j","DOIUrl":"https://doi.org/10.1039/d5lc00540j","url":null,"abstract":"The development of an effective therapy against glioblastoma (GBM) remains a significant and unmet clinical need. To address this challenge, creating predictive, physiologically relevant screening models is essential for accelerating the identification of promising drug candidates. In this paper, we present a novel impedance-based device where two-photon polymerization-fabricated scaffolds embedding electrodes are colonized by glioblastoma cells, effectively replicating the three-dimensional environment of the microscopic tumor foci that persist following tumor resection and cause recurrence. The results demonstrated that the proposed GBM-on-chip model enables high-throughput, multiplexed, and real-time monitoring of tumor spheroid development and their responses to therapeutic agents. Validation studies demonstrated the platform ability to detect subtle cytotoxic effects undetectable by traditional immunofluorescence methods, with optical transparency enabling complementary imaging analysis. This system represents a versatile framework for assessing drug efficacy in complex, physiologically relevant 3D tumor models, paving the way for innovations in cancer pharmacology.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"10 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144645253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bistable magnetic valves for selective sweat sampling in wearable microfluidics 可穿戴微流体中选择性汗液取样的双稳磁阀

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-17 DOI: 10.1039/d5lc00576k

Chaemin Kim, Chanyong Shin, Anna Lee, Jonghyun Ha, Jungil Choi

引用次数: 0

Rapid prototyping of multicompartment Liver-on-Chip for dynamic administration of tumour derived vesicles within an electrospun scaffold 多室肝脏芯片的快速原型设计，用于在电纺丝支架内动态管理肿瘤来源的囊泡

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-17 DOI: 10.1039/d5lc00353a

Maria Testa, Marco Loria, Francesco Lopresti, Chiara Di Marco, Maïwenn Kersaudy-Kerhoas, Fabio Bucchieri, Marzia Pucci, Elisa Costanzo, Simone Scilabra, Riccardo Alessandro, Simona Fontana, V. La Carrubba

{"title":"Rapid prototyping of multicompartment Liver-on-Chip for dynamic administration of tumour derived vesicles within an electrospun scaffold","authors":"Maria Testa, Marco Loria, Francesco Lopresti, Chiara Di Marco, Maïwenn Kersaudy-Kerhoas, Fabio Bucchieri, Marzia Pucci, Elisa Costanzo, Simone Scilabra, Riccardo Alessandro, Simona Fontana, V. La Carrubba","doi":"10.1039/d5lc00353a","DOIUrl":"https://doi.org/10.1039/d5lc00353a","url":null,"abstract":"A novel multicompartment Liver-on-Chip (LoC) platform has been developed combining laser-micromachined poly(methylmethacrylate) (PMMA) layers with an electrospun poly-lactic acid (PLA) scaffold to emulate the liver's extracellular matrix (ECM) for advanced in vitro modeling. The platform supports the dynamic, chronic delivery of colorectal cancer-derived extracellular vesicles (CRC-EVs) under physiologically relevant conditions. The use of thermoplastic materials such as PMMA provides advantages including low absorption, high optical clarity, and reproducibility, while the biomimetic architecture of the PLA scaffold enhances structural and functional fidelity. The LoC platform demonstrates significant advancements over conventional 2D cultures and static systems. Proteomic analyses revealed enhanced hepatocyte differentiation and activation of liver-specific pathways when cells were cultured on the PLA scaffold under both static and dynamic conditions. Dynamic CRC-EV administration induced the upregulation of the mesenchymal marker Vimentin in the hepatocytes, as previously described in the 2D system. This study establishes the LoC as a groundbreaking tool for investigating tumor-liver interactions and pre-metastatic niche formation. By addressing critical limitations of existing platforms, this system advances organ-on-chip technology for cancer research and therapeutic development.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"109 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144652374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication of a bioreactor combining soft lithography and vat photopolymerisation to study tissues and multicellular organisms under dynamic culture conditions. 在动态培养条件下研究组织和多细胞生物的软光刻和还原光聚合相结合的生物反应器的制造。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-16 DOI: 10.1039/d5lc00172b

Thomas Meynard,Félix Royer,Robin Houssier,Orégane Bajeux,Sonia Paget,Fatima Lahdaoui,Alejandra Mogrovejo Valdivia,Nathalie Maubon,Jérôme Vicogne,Isabelle Van Seuningen,Vincent Senez

{"title":"Fabrication of a bioreactor combining soft lithography and vat photopolymerisation to study tissues and multicellular organisms under dynamic culture conditions.","authors":"Thomas Meynard,Félix Royer,Robin Houssier,Orégane Bajeux,Sonia Paget,Fatima Lahdaoui,Alejandra Mogrovejo Valdivia,Nathalie Maubon,Jérôme Vicogne,Isabelle Van Seuningen,Vincent Senez","doi":"10.1039/d5lc00172b","DOIUrl":"https://doi.org/10.1039/d5lc00172b","url":null,"abstract":"Despite its capability to create much more realistic microenvironments for in vitro culturing of animal or human biological models, the spread of microfluidic tools in the world of biology and medicine has still not reached the predicted scale. Major obstacles to their widespread acceptance by end-users are manufacturing cost and operational complexity. 3D printing is a technology that is now widely democratised, thanks to its ease of use and very attractive cost/performance ratio. In particular, photopolymerisation through a liquid crystal screen is experiencing a very significant growth. Here, we describe the methodology we developed to evaluate this microfabrication technique and selected a photoprintable resin to manufacture a fluidic microsystem dedicated to tissue or micro-organism culture. The first originality of our approach lies in the architecture of the microsystem, which is made up of an elementary culture chamber in two parts, making it very easy to open after the culture period to carry out ex situ biochemical analyses. The second one is the nature of the materials used to make up the culture chamber, which consists of polydimethylsiloxane and a photoprinted resin. This hybrid assembly, combining an elastomer and a rigid plastic material, ensures a better seal and better dimensional control once the assembly is complete. We demonstrate the ability of our protocol to flexibly fabricate different culture chambers with dimensions down to 100 microns and we show for one of them three applications: a 2D layer of cell lines, a parasitic worm and a 3D microtissue from a pancreatic cancer patient.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"37 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144640306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D Modelling of Pulmonary Arterial Stenosis and Endothelial Dysfunction in CTEPH CTEPH肺动脉狭窄和内皮功能障碍的三维建模

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-16 DOI: 10.1039/d5lc00300h

Salina Nicoleau, Ylenia Roger Valle, Olga Tura-Ceide, Chloe Armour, Joan Albert Barbera, A. J. McKinnon Thomas, Deepa Gopalan, Beata Wojciak-Stothard

{"title":"3D Modelling of Pulmonary Arterial Stenosis and Endothelial Dysfunction in CTEPH","authors":"Salina Nicoleau, Ylenia Roger Valle, Olga Tura-Ceide, Chloe Armour, Joan Albert Barbera, A. J. McKinnon Thomas, Deepa Gopalan, Beata Wojciak-Stothard","doi":"10.1039/d5lc00300h","DOIUrl":"https://doi.org/10.1039/d5lc00300h","url":null,"abstract":"Chronic thromboembolic pulmonary hypertension (CTEPH) arises from progressive thrombotic occlusion of pulmonary arteries, involving vessel blockage by unresolved thrombi and small vessel arteriopathy. This disrupts blood flow, increases lung pressure, and alters vessel geometry, contributing to endothelial dysfunction. However, the mechanisms remain unclear. To study these interactions, we developed microfluidic 3D models of pulmonary arteries with 30–80% stenosis using CTPAs from CTEPH and acute pulmonary embolism (APE) patients, in silico flow simulations, 3D printing, and soft lithography. Unlike standard circular channels, we designed semi-circular channels enclosed by a coverslip, which computational modelling confirmed closely mimics real vessel flow dynamics. Human pulmonary artery endothelial cells (HPAECs) cultured in 30–80% stenosis channels exhibited increased expression of pro-inflammatory, pro-thrombotic, and pro-angiogenic genes, with responses varying by stenosis severity and location. Cells in post-stenotic dilatation regions (60–80% stenosis) lost alignment and junctional integrity due to disturbed flow. The transcriptional profile of HPAECs from 80% stenosis channels closely resembled that of CTEPH pulmonary endarterectomy specimens. Platelet adhesion, dependent on von Willebrand factor (VWF), varied with stenosis severity and flow rate. Low perfusion rates increased adhesion in stenotic regions, while higher flow rates promoted adhesion post-stenosis. Our patient data-based stenosis models provide a robust platform for studying the effects of vascular geometry on blood flow, endothelial responses, and platelet aggregation, advancing research on CTEPH, pulmonary embolism, and other diseases associated with vascular occlusion.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"109 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144645254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Teach your microscope how to print: low-cost and rapid-iteration microfabrication for biology 教你的显微镜如何打印：低成本和快速迭代的生物微加工

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-14 DOI: 10.1039/d5lc00181a

Lucien Hinderling, Remo Hadorn, Moritz Kwasny, Joël Frei, Benjamin Grädel, Sacha Psalmon, Yannick Blum, Rémi Berthoz, Alex E. Landolt, Benjamin D. Towbin, Daniel Riveline, Olivier Pertz

{"title":"Teach your microscope how to print: low-cost and rapid-iteration microfabrication for biology","authors":"Lucien Hinderling, Remo Hadorn, Moritz Kwasny, Joël Frei, Benjamin Grädel, Sacha Psalmon, Yannick Blum, Rémi Berthoz, Alex E. Landolt, Benjamin D. Towbin, Daniel Riveline, Olivier Pertz","doi":"10.1039/d5lc00181a","DOIUrl":"https://doi.org/10.1039/d5lc00181a","url":null,"abstract":"The application of traditional microfabrication techniques to biological research is hindered by their reliance on clean rooms, expensive or toxic materials, and slow iteration cycles. We present an accessible microfabrication workflow that addresses these challenges by integrating consumer 3D printing techniques and repurposing standard fluorescence microscopes equipped with DMDs for maskless photolithography. Our method achieves micrometer-scale precision across centimeter-sized areas without clean room infrastructure, using affordable and readily available consumables. We demonstrate the versatility of this approach through four biological applications: inducing cytoskeletal protrusions via 1 μm-resolution surface topographies; micropatterning to standardize cell and tissue morphology; fabricating multilayer microfluidic devices for confined cell migration studies; imprinting agar chambers for long-time tracking of C. elegans. Our protocol drastically reduces material costs compared to conventional methods and enables design-to-device turnaround within a day. By leveraging open-source microscope control software and existing lab equipment, our workflow lowers the entry barrier to microfabrication, enabling labs to prototype custom solutions for diverse experimental needs while maintaining compatibility with soft lithography and downstream biological assays.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"21 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144622724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A urine detection chip for the analysis of urinary cells and extracellular vesicles for bladder cancer screening 一种用于膀胱癌筛查的尿细胞和细胞外囊泡分析的尿液检测芯片

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-14 DOI: 10.1039/d5lc00511f

Junyuan Liu, Yuxin Qu, Xun Xu, Zixing Ye, Jing Cheng, Han Wang

引用次数: 0

Diagnostic technologies for neuroblastoma 神经母细胞瘤的诊断技术

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-14 DOI: 10.1039/D4LC00005F

Leena Khelifa, Yubing Hu, Jennifer Tall, Rasha Khelifa, Amina Ali, Evon Poon, Mohamed Zaki Khelifa, Guowei Yang, Catarina Jones, Rosalia Moreddu, Nan Jiang, Savas Tasoglu, Louis Chesler and Ali K. Yetisen

{"title":"Diagnostic technologies for neuroblastoma","authors":"Leena Khelifa, Yubing Hu, Jennifer Tall, Rasha Khelifa, Amina Ali, Evon Poon, Mohamed Zaki Khelifa, Guowei Yang, Catarina Jones, Rosalia Moreddu, Nan Jiang, Savas Tasoglu, Louis Chesler and Ali K. Yetisen","doi":"10.1039/D4LC00005F","DOIUrl":"10.1039/D4LC00005F","url":null,"abstract":"Neuroblastoma is an aggressive childhood cancer characterised by high relapse rates and heterogenicity. Current medical diagnostic methods involve an array of techniques, from blood tests to tumour biopsies. This process is associated with long-term physical and psychological trauma. Moreover, current technologies do not identify neuroblastoma at an early stage while tumours are easily resectable. In recent decades, many advancements have been made for neuroblastoma diagnosis, including liquid biopsy platforms, radiomics, artificial intelligence (AI) integration and biosensor technologies. These innovations support the trend towards rapid, non-invasive and cost-effective diagnostic methods which can be utilised for accurate risk stratification. Point-of-care (POC) diagnostic devices enable rapid and accurate detection of disease biomarkers and can be performed at the location of the patient. Whilst POC diagnostics has been well-researched within the oncological landscape, few devices have been reported for neuroblastoma, and these remain in the early research phase and as such are limited by lack of clinical validation. Recent research has revealed several potential biomarkers which have great translational potential for POC diagnosis, including proteomic, metabolic and epigenetic markers such as MYCN amplification and microRNAs (miRNAs). Using POC devices to detect high-risk biomarkers in biofluids such as blood and urine, offers a non-invasive approach to diagnosis of neuroblastoma, enabling early screening at a population level as well as real-time health monitoring at home. This is critical to mitigating long-term morbidity associated with late diagnosis and unnecessary treatment, in turn improving outcomes for neuroblastoma patients.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 15","pages":" 3630-3664"},"PeriodicalIF":6.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d4lc00005f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144622725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High speed microturbine mixer for kinetically controlled synthesis 用于动控合成的高速微涡轮混合器

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-11 DOI: 10.1039/d5lc00488h

Avery E. England, Scott D. Collins, Christopher L. Emmerling, Michael D. Mason, Rosemary L. Smith

{"title":"High speed microturbine mixer for kinetically controlled synthesis","authors":"Avery E. England, Scott D. Collins, Christopher L. Emmerling, Michael D. Mason, Rosemary L. Smith","doi":"10.1039/d5lc00488h","DOIUrl":"https://doi.org/10.1039/d5lc00488h","url":null,"abstract":"The design, fabrication, simulation, and experimental characterization of a microfabricated, fluidically-driven microturbine mixer are presented. The mixer was engineered to achieve rapid mixing (<1 ms), enabling control over kinetically-limited chemical reactions. The microturbine is microfabricated in silicon using a sequence of photolithographic patterning and deep reactive ion etching (DRIE) steps. The device features two fluidic inlets, each supplying chemical reagents, that drive a microturbine through momentum transfer, generating shear forces within the reaction chamber to induce mixing. By systematically varying the flow rates of the reagents, the rotational velocities of the microturbine were experimentally and computationally determined. Mixing profiles were analyzed using fluorescence colocalization, an established biological imaging technique that was adopted for this application. Characterization results were leveraged to optimize the synthesis of ultra-small, monodisperse silver nanoparticles (AgNPs), i.e. particles of 1 nm to 3 nm in diameter. These nanoparticles have very large surface-to-volume ratios, making them ideal candidates for applications in catalysis, sensing, and antimicrobial agents. The microturbine mixer provides a scalable and reproducible method for the production of ultra-small AgNPs through precise control of mixing conditions, overcoming challenges associated with traditional synthesis routes that struggle to attain size control, size distribution and reproducibility.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"32 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144603747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0