Lab on a Chip最新文献_第2页

A vascularized microfluidic model of the osteochondral unit for modeling inflammatory response and therapeutic screening. 用于炎症反应建模和治疗筛选的骨软骨单元血管化微流体模型。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-01-28 DOI: 10.1039/d4lc00651h

Kevin D Roehm, Irene Chiesa, Dustin Haithcock, Riccardo Gottardi, Balabhaskar Prabhakarpandian

引用次数: 0

Injury-on-a-chip for modelling microvascular trauma-induced coagulation. 用于微血管创伤性凝血建模的芯片损伤技术。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-01-28 DOI: 10.1039/d4lc00471j

Halston Deal, Elizabeth M Byrnes, Sanika Pandit, Anastasia Sheridan, Ashley C Brown, Michael Daniele

{"title":"Injury-on-a-chip for modelling microvascular trauma-induced coagulation.","authors":"Halston Deal, Elizabeth M Byrnes, Sanika Pandit, Anastasia Sheridan, Ashley C Brown, Michael Daniele","doi":"10.1039/d4lc00471j","DOIUrl":"10.1039/d4lc00471j","url":null,"abstract":"Blood coagulation is a highly regulated injury response that features polymerization of fibrin fibers to prevent the passage of blood from a damaged vascular endothelium. A growing body of research seeks to monitor coagulation in microfluidic systems but fails to capture coagulation as a response to disruption of the vascular endothelium. Here we present a device that allows compression injury of a defined segment of a microfluidic vascular endothelium and the assessment of coagulation at the injury site. This pressure injury-on-a-chip (PINCH) device allows visualization of coagulation as the accumulation of fluorescent fibrin at injury sites. Quantification of fluorescent fibrin levels upstream of and at injury sites confirm that pre-treating vascular endothelium with fluid shear stress helps capture coagulation as an injury response. We leverage the PINCH devices to demonstrate the limited coagulation response of type A hemophiliacs and evaluate the performance of hemostatic microparticles and fibrinolytic nanoparticles. Our findings and the straightforward fabrication of the PINCH devices make it a promising choice for additional screening of hemostatic therapeutics.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":"440-453"},"PeriodicalIF":6.1,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11704661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An intimal-lumen model in a microfluidic device: potential platform for atherosclerosis-related studies. 微流控装置中的内膜-腔模型：动脉粥样硬化相关研究的潜在平台。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-01-28 DOI: 10.1039/d4lc00868e

Fahima Akther, Dimple Sajin, Shehzahdi S Moonshi, Jessica Pickett, Yuao Wu, Jun Zhang, Nam-Trung Nguyen, Hang Thu Ta

{"title":"An intimal-lumen model in a microfluidic device: potential platform for atherosclerosis-related studies.","authors":"Fahima Akther, Dimple Sajin, Shehzahdi S Moonshi, Jessica Pickett, Yuao Wu, Jun Zhang, Nam-Trung Nguyen, Hang Thu Ta","doi":"10.1039/d4lc00868e","DOIUrl":"10.1039/d4lc00868e","url":null,"abstract":"Atherosclerosis is a chronic inflammatory vascular disorder driven by factors such as endothelial dysfunction, hypertension, hyperlipidemia, and arterial calcification, and is considered a leading global cause of death. Existing atherosclerosis models have limitations due to the absence of an appropriate hemodynamic microenvironment in vitro and interspecies differences in vivo. Here, we develop a simple but robust microfluidic intimal-lumen model of early atherosclerosis using interconnected dual channels for studying monocyte transmigration and foam cell formation at an arterial shear rate. To the best of our knowledge, this is the first study that creates a physiologically relevant microenvironment under an arterial shear rate to modulate lipid-laden foam cells on a microfluidic platform. As a proof of concept, we use murine endothelial cells to develop a vascular lumen in one channel and collagen-embedded murine smooth muscle cells to mimic the subendothelial intimal layer in another channel. The model successfully triggers endothelial dysfunction upon TNF-α stimulation, initiating monocyte adhesion to the endothelial monolayer under the arterial shear rate. Unlike existing in vitro models, native low-density lipoprotein (LDL) is added in the culture media instead of ox-LDL to stimulate subendothelial lipid accumulation, thereby mimicking more accurate physiology. The subendothelial transmigration of adherent monocytes and subsequent foam cell formation is also achieved under flow conditions in the model. The model also investigates the inhibitory effect of aspirin in monocyte adhesion and transmigration. The model exhibits a significant dose-dependent reduction in monocyte adhesion and transmigration upon aspirin treatment, making it an excellent tool for drug testing.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":"354-369"},"PeriodicalIF":6.1,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liquid metal electrodes enabled cascaded on-chip dielectrophoretic separation of large-size-range particles. 液态金属电极使级联片上介电分离的大尺寸范围的颗粒。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-01-28 DOI: 10.1039/d4lc00942h

Huichao Chai, Liang Huang, Junwen Zhu, Jialu Tian, Wenhui Wang

{"title":"Liquid metal electrodes enabled cascaded on-chip dielectrophoretic separation of large-size-range particles.","authors":"Huichao Chai, Liang Huang, Junwen Zhu, Jialu Tian, Wenhui Wang","doi":"10.1039/d4lc00942h","DOIUrl":"10.1039/d4lc00942h","url":null,"abstract":"The separation of large-size-range particles of complex biological samples is critical but yet well resolved. As a label-free technique, dielectrophoresis (DEP)-based particle separation faces the challenge of how to configure DEP in an integrated microfluidic device to bring particles of various sizes into the effective DEP force field. Herein, we propose a concept that combines the passive flow fraction mechanism with the accumulative DEP deflection effect in a cascaded manner. This concept places DEP deflection segments and bypass outlets alternately. Each DEP deflection segment is configured with an array of side-wall liquid metal electrodes to exert effective DEP forces on the particles of a suitable size range. After each DEP deflection segment, the passive bypass flow fraction mechanism diverts part of the sample flow and target range of particles through the bypass outlet. Simultaneously, this flow fraction brings the remaining particles closer to the electrodes in the subsequent DEP deflection segment, causing the next size range of particles to deflect under effective DEP forces and thus making them separable. Repeating this process, particles would be separated from the bypass outlets one by one in the order of reducing size ranges. We present the concept design and modeling, and prove the concept through separating five different particles ranging from 16-0.5 μm mixed together to mimic blood composition, providing a powerful platform for separating multiple particles in diverse biomedical applications.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":"308-318"},"PeriodicalIF":6.1,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidics for morpholomics and spatial omics applications.

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-01-27 DOI: 10.1039/d4lc00869c

Nishanth Venugopal Menon, Jeeyeon Lee, Tao Tang, Chwee Teck Lim

引用次数: 0

Point-of-need diagnostics in a post-Covid world: an opportunity for paper-based microfluidics to serve during syndemics.

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-01-23 DOI: 10.1039/d4lc00699b

Maria-Nefeli Tsaloglou, Dionysios C Christodouleas, Jonathan Milette, Kendall Milkey, Isabelle C Romine, Judy Im, Shefali Lathwal, Duraipandian Thava Selvam, Hadley D Sikes, George M Whitesides

{"title":"Point-of-need diagnostics in a post-Covid world: an opportunity for paper-based microfluidics to serve during syndemics.","authors":"Maria-Nefeli Tsaloglou, Dionysios C Christodouleas, Jonathan Milette, Kendall Milkey, Isabelle C Romine, Judy Im, Shefali Lathwal, Duraipandian Thava Selvam, Hadley D Sikes, George M Whitesides","doi":"10.1039/d4lc00699b","DOIUrl":"https://doi.org/10.1039/d4lc00699b","url":null,"abstract":"Zoonotic outbreaks present with unpredictable threats to human health, food production, biodiversity, national security and disrupt the global economy. The COVID-19 pandemic-caused by zoonotic coronavirus, SARS-CoV2- is the most recent upsurge of an increasing trend in outbreaks for the past 100 years. This year, emergence of avian influenza (H5N1) is a stark reminder of the need for national and international pandemic preparedness. Tools for threat reduction include consistent practices in reporting pandemics, and widespread availability of accurate detection technologies. Wars and extreme climate events redouble the need for fast, adaptable and affordable diagnostics at the point of need. During the recent pandemic, rapid home tests for SARS-CoV-2 proved to be a viable functional model that leverages simplicity. In this perspective, we introduce the concept of syndemnicity in the context of infectious diseases and point-of-need healthcare diagnostics. We also provide a brief state-of-the-art for paper-based microfluidics. We illustrate our arguments with a case study for detecting brucellosis in cows. Finally, we conclude with lessons learned, challenges and opportunities for paper-based microfluidics to serve point-of-need healthcare diagnostics during syndemics.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic vessel-on-chip platform for investigation of cellular defects in venous malformations and responses to various shear stress and flow conditions.

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-01-23 DOI: 10.1039/d4lc00824c

Mohammadhassan Ansarizadeh, Hoang-Tuan Nguyen, Bojana Lazovic, Jere Kettunen, Laknee De Silva, Ragul Sivakumar, Pauliina Junttila, Siiri-Liisa Rissanen, Ryan Hicks, Prateek Singh, Lauri Eklund

{"title":"Microfluidic vessel-on-chip platform for investigation of cellular defects in venous malformations and responses to various shear stress and flow conditions.","authors":"Mohammadhassan Ansarizadeh, Hoang-Tuan Nguyen, Bojana Lazovic, Jere Kettunen, Laknee De Silva, Ragul Sivakumar, Pauliina Junttila, Siiri-Liisa Rissanen, Ryan Hicks, Prateek Singh, Lauri Eklund","doi":"10.1039/d4lc00824c","DOIUrl":"https://doi.org/10.1039/d4lc00824c","url":null,"abstract":"A novel microfluidic platform was designed to study the cellular architecture of endothelial cells (ECs) in an environment replicating the 3D organization and flow of blood vessels. In particular, the platform was constructed to investigate EC defects in slow-flow venous malformations (VMs) under varying shear stress and flow conditions. The platform featured a standard microtiter plate footprint containing 32 microfluidic units capable of replicating wall shear stress (WSS) in normal veins and enabling precise control of shear stress and flow directionality without the need for complex pumping systems. Using genetically engineered human umbilical vein endothelial cells (HUVECs) and induced pluripotent stem cell (iPSC)-derived ECs (iECs) to express the recurrent TIE2L914F VM mutation we assessed responses on EC orientation and area, actin organization, and Golgi polarization to uni- and bidirectional flow and varying WSS. Comparison of control and TIE2L914F expressing ECs showed differential cellular responses to flow and WSS in terms of cell shape elongation, orientation of F-actin, and Golgi polarization, indicating altered mechanosensory or mechanotransduction signaling pathways in the presence of the VM causative mutation. The data also revealed significant differences in how the primary and iPSC-derived iECs responded to flow. As a conclusion, the developed microfluidic platform allowed simulation of multiple flow conditions in a scalable and pumpless format. The design made it a desirable tool for studying different EC types as well as cellular changes in vascular disease. The platform should offer new opportunities for biomechanical research by providing a controlled environment to analyze the flow-dependent mechanosensory pathways in ECs.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring microfluidics-based organoid interactions through analysis of albumin secretion. 通过分析白蛋白分泌，探索基于微流体的类器官相互作用。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-01-22 DOI: 10.1039/d4lc01085j

Yang Yang, Yueyang Qu, Jing Wang, Yuxiu Wang, Jiamin Zhao, Miaomiao Wang, Wanqing Hu, Jiaqi Zhao, Bingcheng Lin, Xiuli Zhang, Yong Luo

{"title":"Exploring microfluidics-based organoid interactions through analysis of albumin secretion.","authors":"Yang Yang, Yueyang Qu, Jing Wang, Yuxiu Wang, Jiamin Zhao, Miaomiao Wang, Wanqing Hu, Jiaqi Zhao, Bingcheng Lin, Xiuli Zhang, Yong Luo","doi":"10.1039/d4lc01085j","DOIUrl":"https://doi.org/10.1039/d4lc01085j","url":null,"abstract":"Organoids-on-a-chip exhibit significant potential for advancing disease modeling, drug screening, and precision medicine, largely due to their capacity to facilitate interactions among organoids. However, the influence of chip design on these interactions remains poorly understood, primarily due to our limited knowledge of the mediators of communication and the complexity of interaction dynamics. This study demonstrates that analyzing albumin secretion from liver organoids within an organoids-on-a-chip system can provide a measure of the interaction intensity among organoids, offering valuable insights into how chip design influences these interactions. Our findings reveal that the interaction dynamics of target organoids is primarily affected by the types of neighboring organoids positioned upstream. For instance, adipose organoids located upstream and adjacent to liver organoids considerably stimulate functional improvements in the liver organoids, whereas adipose organoids in other arrangements do not produce similar effects. Importantly, both theoretical and experimental evidence indicate that the interaction dynamics is independent of the physical distance between organoids. Instead, it can be adjusted by flow rate, well depth, introducing a vascular barrier, or the media volume within the system. However, it is crucial to note that the influence of these factors is not linear. Finally, the exosome was identified as one of key mediators of communication within the organoids-on-a-chip system.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142996521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A single droplet dispensing system for high-throughput screening and reliable recovery of rare events. 单滴点胶系统用于高通量筛选和可靠的罕见事件恢复。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-01-21 DOI: 10.1039/d4lc00536h

Marian Weiss,Sadat Hasan,Robert Genth,Mohammad Mollah,Elea Robert,Alejandro Gil,Lars Hufnagel

{"title":"A single droplet dispensing system for high-throughput screening and reliable recovery of rare events.","authors":"Marian Weiss,Sadat Hasan,Robert Genth,Mohammad Mollah,Elea Robert,Alejandro Gil,Lars Hufnagel","doi":"10.1039/d4lc00536h","DOIUrl":"https://doi.org/10.1039/d4lc00536h","url":null,"abstract":"Microfluidic droplet sorting has emerged as a powerful technique for a broad spectrum of biomedical applications ranging from single cell analysis to high-throughput drug screening, biomarker detection and tissue engineering. However, the controlled and reliable retrieval of selected droplets for further off-chip analysis and processing is a significant challenge in droplet sorting, particularly in high-throughput applications with low expected hit rates. In this study, we present a microfluidic platform capable of sorting and dispensing individual droplets with minimal loss rates. We demonstrate our direct transfer mechanism by placing selected droplets containing hybridoma cells into microwells, eliminating the need for manual and often lossy handling steps. Sorted droplets are dispensed via a novel 3D-printed dispensing nozzle, enabling precise and controlled placement of selected single droplets into individual wells without affecting the microfluidic sorting flow. The sorting and transfer process is monitored in real time, which provides feedback and quality control of the entire workflow. Our integrated microfluidic system holds great potential for applications requiring high-throughput droplet sorting with minimal sample loss and precise dispensing into microwells, such as screening for therapeutical antibodies or monoclonal cells.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"24 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142991804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The reversible capillary field effect transistor: a capillaric element for autonomous flow switching. 可逆毛细管场效应晶体管：一种用于自主流量开关的毛细管元件。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-01-17 DOI: 10.1039/d4lc00706a

Daniel Mak,Claude Meffan,Julian Menges,Rhys Marchant-Ludlow,Azadeh Hashemi,Ciaran P Moore,Renwick C J Dobson,Volker Nock

{"title":"The reversible capillary field effect transistor: a capillaric element for autonomous flow switching.","authors":"Daniel Mak,Claude Meffan,Julian Menges,Rhys Marchant-Ludlow,Azadeh Hashemi,Ciaran P Moore,Renwick C J Dobson,Volker Nock","doi":"10.1039/d4lc00706a","DOIUrl":"https://doi.org/10.1039/d4lc00706a","url":null,"abstract":"New flow control elements in capillaric circuits are key to achieving ever more complex lab-on-a-chip functionality while maintaining their autonomous and easy-to-use nature. Capillary field effect transistors valves allow for flow in channels to be restricted and cut off utilising a high pressure triggering channel and occluding air bubble. The reversible capillary field effect transistor presented here provides a new element that can restore fluid flow in closed microchannels via autonomous circuit feedback. This allows new flow switching functionality without the need for direct user input. The valve design utilises new circuitry that draws on competing capillary pressures to withdraw liquid from a reservoir connected to the valve, creating a suction pressure that removes the occluding bubble from the channel to allow flow past the valve. The resulting reopening restores flow to the closed channel and allows for enhanced autonomous control over fluid flows. This new functionality is flexible and has the potential to be applied in a wide variety of situations, as shown here by use in several extended proof of concept arrangements. Firstly, we demonstrate how to reopen one valve while closing another using the same trigger to achieve simultaneous flow switching. We then show how a single trigger can be used for the parallel reopening of multiple valves for simultaneous release of liquids. Finally, we show the reversible capillary field effect transistor used to achieve autonomous transient mixing ratios between multiple liquids utilising a series of triggering events to determine which liquid channels are open or closed as flow progresses. The functionality this valve adds to the capillaric toolbox opens up new possibilities for applications in the creation of fully automatic diagnostic capillaric devices.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"24 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142989077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0