Lab on a Chip最新文献_第2页

Microfluidic osmotic compression with operando meso-structure characterization using SAXS. 微流控渗透压缩中operando介结构的SAXS表征。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-04-15 DOI: 10.1039/d4lc01087f

Dimitri Radajewski,Pierre Roblin,Patrice Bacchin,Martine Meireles,Yannick Hallez

{"title":"Microfluidic osmotic compression with operando meso-structure characterization using SAXS.","authors":"Dimitri Radajewski,Pierre Roblin,Patrice Bacchin,Martine Meireles,Yannick Hallez","doi":"10.1039/d4lc01087f","DOIUrl":"https://doi.org/10.1039/d4lc01087f","url":null,"abstract":"We have developed a microfluidic chip for the osmotic compression of samples at the nanoliter scale, enabling the in situ and operando acquisition of structural features through small-angle X-ray scattering throughout the compression process. The design builds upon a previous setup allowing high-throughput measurements with minimal sample quantities. The updated design is specifically tailored for compatibility with a laboratory beamline, taking into account factors such as reduced photon flux and increased beam size compared to synchrotron beamlines. As a proof of concept, we performed on-chip compression of well-documented silica colloidal particles (Ludox TM-50). We demonstrated that the volume fraction could be tracked over time during compression, either by monitoring X-ray absorbance or by modeling the scattered signal. With precise control of the osmotic pressure and salt chemical potential, equations of state can be determined unambiguously from the volume fraction measurements and be interpreted with the help of the scattered intensity. These microfluidic chips will be valuable for understanding the behavior of colloidal suspensions, with applications in areas such as crystallization, nucleation, soil mechanics, control of living matter growth and interaction conditions, as well as the measurement of coarse-grained colloidal interaction potentials.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"338 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143836482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual lateral flow assay using quantum nanobeads for quantitative detection of BDNF and TNF-α in tears. 量子纳米珠双侧流法定量检测泪液中BDNF和TNF-α。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-04-15 DOI: 10.1039/d4lc01045k

Yue Wu,Yubing Hu,Nan Jiang,Maria W Georgi,Ali K Yetisen,M Francesca Cordeiro

{"title":"Dual lateral flow assay using quantum nanobeads for quantitative detection of BDNF and TNF-α in tears.","authors":"Yue Wu,Yubing Hu,Nan Jiang,Maria W Georgi,Ali K Yetisen,M Francesca Cordeiro","doi":"10.1039/d4lc01045k","DOIUrl":"https://doi.org/10.1039/d4lc01045k","url":null,"abstract":"Glaucoma is a group of neurodegenerative eye diseases characterized by progressive damage to the optic nerve which is typically asymptomatic until irreversible vision loss has occurred. Early screening is essential for timely treatment to prevent visual impairment. However, existing detection methods struggle to achieve a balance between accuracy, time efficiency, and portability. The lateral flow assay (LFA) is a well-established immunoanalytical tool for point-of-care (POC) analysis. Here, we have developed a unique dual-testing, quantum nanobeads-based fluorescence LFA, allowing for the simultaneous and quantitative detection of two glaucoma biomarkers: tumor necrosis factor-α (TNF-α) and brain-derived neurotrophic factor (BDNF). By coating quantum dots on the surface of a SiO2 core, the fluorescent intensity of the quantum nanobeads was enhanced enabling an accurate, efficient, and high-throughput bioanalytical performance, with low detection limits of 3.39 pg mL-1 for TNF-α and 4.13 pg mL-1 for BDNF. The LFA also demonstrated superior selectivity, reproducibility, and stability to the standard enzyme-linked immunosorbent assay (ELISA). Using a 3D-printed readout box, the analysis of the LFA requires only a readily accessible smartphone and image processing software, making it an ideal POC detection tool. This ultrasensitive, economical, and user-friendly LFA demonstrates significant potential as an alternative for glaucoma screening.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"1 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143836487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chiral organic-inorganic hybrid perovskites synthesized using an acoustofluidic closed system. 利用声流体封闭系统合成手性有机-无机杂化过氧化物。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-04-14 DOI: 10.1039/d4lc01073f

Tao Zhou,Yan Yu,Haonan Zhang,Chong Li,Ran Tao,Fujian Ren,Chen Fu,Jingting Luo,Yongqing Fu

引用次数: 0

Bioselective agglutination induced nanoscale deterministic lateral displacement. 生物选择性凝集诱导纳米级确定性横向位移。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-04-14 DOI: 10.1039/d5lc00079c

Kuan Yu Hsieh,Joshua T Smith,Sung-Cheol Kim,Stacey M Gifford,Michael Pereira,Guan-Yu Chen,Benjamin H Wunsch

引用次数: 0

A novel microfluidic self-perfusion chip (MSPC) for pumpless 3D cell, microtissue and organoid culture. 一种新型微流控自灌注芯片（MSPC），用于无泵三维细胞、显微组织和类器官培养。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-04-10 DOI: 10.1039/d5lc00030k

Guohua Wu,Di Wu,Wenqi Hu,Qinrui Lu,Yusen Zhou,Jie Liu,Qijun Du,Zhi Luo,Haijie Hu,Hongwei Jiang,Bangchuan Hu,Shuqi Wang

{"title":"A novel microfluidic self-perfusion chip (MSPC) for pumpless 3D cell, microtissue and organoid culture.","authors":"Guohua Wu,Di Wu,Wenqi Hu,Qinrui Lu,Yusen Zhou,Jie Liu,Qijun Du,Zhi Luo,Haijie Hu,Hongwei Jiang,Bangchuan Hu,Shuqi Wang","doi":"10.1039/d5lc00030k","DOIUrl":"https://doi.org/10.1039/d5lc00030k","url":null,"abstract":"Microfluidic systems have revolutionized biological research by enabling precise control over cellular environments at microscale volumes. However, traditional pump-driven systems face challenges such as complexity, cost, cell-damaging shear stress, and limited portability. This study introduces a novel adjustable microfluidic self-perfusion chip (MSPC) that uses evaporation as a driving force, eliminating the need for external pumps. Our design offers improved metabolic waste management and simplified control over fluid dynamics. The chip features adjustable evaporation pore sizes, demonstrating a robust linear relationship (R2 = 0.95) between the pore size and fluid evaporation rate. This ensures consistent fluid flow and effective waste removal, shown by lower ammonia and lactate levels compared to conventional cultures. Its unidirectional flow system and integrated one-way valve maintain cell viability, even under complete evaporation conditions. This innovative platform facilitates the cultivation of complex tissue-like structures, providing a valuable tool for tissue and organ model development, as well as drug screening and toxicity testing. By addressing key limitations of traditional systems, our adjustable MSPC represents a significant advancement in microfluidic cell culture technology, offering improved accessibility and applicability in biological research.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"106 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143819382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements in microfluidic technology for rapid bacterial detection and inflammation-driven diseases. 微流体技术在细菌快速检测和炎症驱动疾病中的进展。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-04-09 DOI: 10.1039/d4lc00795f

Jing Zhang, Yatian Fu, Ching Yin Fong, Haojun Hua, Wei Li, Bee Luan Khoo

{"title":"Advancements in microfluidic technology for rapid bacterial detection and inflammation-driven diseases.","authors":"Jing Zhang, Yatian Fu, Ching Yin Fong, Haojun Hua, Wei Li, Bee Luan Khoo","doi":"10.1039/d4lc00795f","DOIUrl":"https://doi.org/10.1039/d4lc00795f","url":null,"abstract":"Bacterial detection is pivotal for the timely diagnosis and effective treatment of infectious diseases. Microfluidic platforms offer advantages over traditional methods, including heightened sensitivity, rapid analysis, and minimal sample volume requirements. Traditional clinical methods for bacterial identification often involve extended processing times and necessitate high pathogen concentrations, resulting in delayed diagnoses and missed treatment opportunities. Microfluidic technology overcomes these limitations by facilitating rapid bacterial identification at lower biomass levels, thus ensuring prompt and precise treatment interventions. Additionally, bacteria-driven inflammation has been associated with the development and progression of various diseases, including cancer. Elucidating the complex interplay between bacteria, inflammation, and disease is essential for devising effective disease models and therapeutic strategies. Microfluidic platforms have been used to construct in vitro disease models that accurately replicate the intricate microenvironment that bacteria-driven inflammation affects. These models offer valuable insights into bacteria-driven inflammation and its impact on disease progression, such as cancer metastasis and therapeutic responses. This review examines recent advancements in bacterial detection using microfluidics and assesses the potential of this technology as a robust tool for exploring bacteria-driven inflammation in the context of cancer.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Mechanical forces and enzymatic digestion act together to induce the remodeling of collagen fibrils in tumor microenvironment. 纠正：机械力和酶消化共同作用，诱导肿瘤微环境中胶原原纤维的重塑。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-04-09 DOI: 10.1039/d5lc90037a

Jiling Shi, Aihua Jing, Qinan Yin, Xuewei Zheng, Zhigang Hu, Xibin Jiao, Yaomin Fan, Xiangyang Zu, Jinghua Li, Yanping Liu, Jiayu Zhai, Xiucheng Li, Kena Song

引用次数: 0

On-chip colorimetric assay for determining serum lithium concentration from whole blood. 片上比色法测定全血中血清锂浓度。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-04-09 DOI: 10.1039/d5lc00044k

Carl Olsson, Janosch Hauser, Federico Ribet, Fredrik Wikström, André Görgens, Olof Beck, Martin Schalling, Lena Backlund, Niclas Roxhed

{"title":"On-chip colorimetric assay for determining serum lithium concentration from whole blood.","authors":"Carl Olsson, Janosch Hauser, Federico Ribet, Fredrik Wikström, André Görgens, Olof Beck, Martin Schalling, Lena Backlund, Niclas Roxhed","doi":"10.1039/d5lc00044k","DOIUrl":"https://doi.org/10.1039/d5lc00044k","url":null,"abstract":"Lithium is the first-line treatment for bipolar disorder. However, the narrow therapeutic window of serum (s-)lithium is near its toxicity range, necessitating continuous monitoring of patients, a process involving regular hospital visits. On-demand home sampling could allow for more frequent testing, possibly resulting in safer patient outcomes, further dosage optimization, and increased compliance. This article presents a device that measures the s-lithium concentration from whole blood. The device consists of a single-use cartridge able to conduct on-chip serum filtration, volume-metering and an on-chip colorimetric assay. Spiked whole blood shows good linearity (Pearson's r = 0.96, R2 = 0.92), a limit-of-detection of 0.3 mmol L-1, and an average deviation of 0.05 mmol L-1 (±6%) compared to atomic absorption spectroscopy. The on-chip colorimetric assay has shown to be a promising technique for measuring s-lithium concentration from whole blood and could allow patients to assess lithium levels at home and make the treatment available for new patient groups.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light-based 3D printing and post-treatments of moulds for PDMS soft lithography. PDMS软光刻模具的光基3D打印及后处理。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-04-07 DOI: 10.1039/d4lc00836g

Bastien Venzac

引用次数: 0

Correction: Functionality integration in stereolithography 3D printed microfluidics using a "print-pause-print" strategy. 更正：使用“打印-暂停-打印”策略的立体光刻3D打印微流体的功能集成。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-04-04 DOI: 10.1039/d5lc90036k

Matthieu Sagot, Timothée Derkenne, Perrine Giunchi, Yohan Davit, Jean-Philippe Nougayrède, Corentin Tregouet, Vincent Raimbault, Laurent Malaquin, Bastien Venzac

引用次数: 0