Lab on a Chip最新文献

{"title":"Scalable and ultrafast CAR-T cell production using microfluidics.","authors":"Vladislav Markelov,Konstantin V Arabuli,Ivan Gaponenko,Vladislav Sergeev,Alena Shakirova,Kirill V Lepik,Alexander D Kulagin,Mikhail V Zyuzin","doi":"10.1039/d5lc00139k","DOIUrl":"https://doi.org/10.1039/d5lc00139k","url":null,"abstract":"Chimeric antigen receptor T cell (CAR-T) therapy has recently gained recognition as a transformative treatment of cancer, particularly of hematological malignancies. However, CAR-T manufacturing remains a major bottleneck of this treatment modality; in standard cases, it takes up to two weeks, resulting in a phenotypic shift toward terminally differentiated T-cells and a significant depletion of T-cells with naive-like phenotype (Tnlp), crucial for sustained clinical efficacy. Leveraging the current progress in microfluidic technologies, we develop and optimize a microfluidic device (MFD) for CAR-T cell production via an ultrafast protocol that integrates T-cell activation and lentiviral transduction in a single step within 24 hours. The MFD geometry allowed reaching a transduction rate of 27% (for MOI 3) compared to 17% and 8% transduction (MOI 3) in 48- and 6-well plates, respectively, used as controls. Notably, in the ultrafast protocol in our MFD, the amount of CD3+ Tnlp is approximately six times higher than that remaining after the standard 9 day protocol (18.07 ± 6.03% vs. 3.97 ± 2.37%). A similar pattern is noted for CD4+ and CD8+ Tnlp, with percentages of 11.07 ± 6.08% vs. 3.56 ± 3.52% and 29.2 ± 7.11% vs. 4.18 ± 1.69%, respectively, in the final CAR-T product. Our results highlight MFDs as a scalable platform to streamline CAR-T manufacturing, with the potential to improve clinical accessibility and outcomes by reducing the production time while preserving essential T-cell phenotypes.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"55 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144103800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LEADS - a comprehensive human liver-on-a-chip for non-alcoholic steatohepatitis (NASH) drug testing.","authors":"Gowri Manohari Balachander,Inn Chuan Ng,Roopesh R Pai,Kartik Mitra,Farah Tasnim,Yee Siang Lim,Royston Kwok,Yoohyun Song,Lai Ping Yaw,Clarissa Bernice Quah,Junzhe Zhao,Wahyunia L Septiana,Vishnu Goutham Kota,Yao Teng,Kexiao Zheng,Yan Xu,Sei Hien Lim,Huck Hui Ng,Hanry Yu","doi":"10.1039/d5lc00221d","DOIUrl":"https://doi.org/10.1039/d5lc00221d","url":null,"abstract":"Metabolic dysfunction associated steatohepatitis (MASH), also known as non-alcoholic steatohepatitis (NASH), is a progressive form of steatotic liver disease (SLD). It is an emerging healthcare threat due its high prevalence, accelerated and non-linear progression, and final culmination as decompensated liver failure and/or hepatocellular carcinoma (HCC). The pathogenesis of NASH is complex with strong ethnic influences and genetic predispositions, underscoring the need for preclinical models that utilize patient-derived cells to enhance our understanding of the disease. Current models face three major limitations: (i) reliance on primary cells with limited reproducibility, high cost, short culture duration and ethical considerations, (ii) failure to recapitulate all key features of NASH, and (iii) inadequate drug testing data and/or data did not correlate with clinical responses. Therefore, there is a pressing need for robust and relevant preclinical models that faithfully recapitulate human NASH, allow generation of patient-specific models and provide quantitative responses for mechanistic studies and drug testing. We have developed a functional liver tissue-on-a-chip by co-culturing human adult liver stem cell (haLSC)-derived hepatobiliary organoids, induced pluripotent stem cell (iPSC)-derived Kupffer cells (iKCs) and iPSC-derived hepatic stellate cells (iHSCs). We simulated the metabolic microenvironment of hyper nutrition and leaky gut by treating the cells with a concoction of free fatty acids (FFAs), fructose, gut-derived lipopolysaccharides (LPS) and a gut-derived metabolite, phenyl acetic acid (PAA). Through optimization of co-culture media and induction regimens, we were able to stably induce steatosis, hepatocellular ballooning, inflammation, and activation of iHSC and fibrosis-all key hallmarks of NASH. Our LEADS (liver-on-a-chip for NASH drug testing) model also recapitulated the pathological types of steatosis and allowed for quantification of the key features via microscopic evaluation and secretome profiling to score for disease severity. Notably, treatment with saroglitazar, pioglitazone, cenicriviroc (CVC), obeticholic acid (OCA) and resmetirom produced responses similar to those observed in clinical trials. Taken together, our LEADS model is the first model developed using patient-derived hepatic stem cells which recapitulated all key features used for comprehensive drug testing, with results matching to clinical responses.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144103532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human mesofluidic intestinal model for studying transport of drug carriers and bacteria through a live mucosal barrier.","authors":"Chia-Ming Wang, Hardeep S Oberoi, Devalina Law, Yuan Li, Timothy Kassis, Linda G Griffith, David T Breault, Rebecca L Carrier","doi":"10.1039/d4lc00774c","DOIUrl":"10.1039/d4lc00774c","url":null,"abstract":"<p><p>The intestinal mucosal barrier forms a critical interface between lumen contents such as bacteria, drugs, and drug carriers and the underlying tissue. Current <i>in vitro</i> intestinal models, while recapitulating certain aspects of this barrier, generally present challenges with respect to imaging transport across mucus and uptake into enterocytes. A human mesofluidic small intestinal chip was designed to enable facile visualization of a mucosal interface created by growing primary human intestinal cells on a vertical hydrogel wall separating channels representing the intestinal lumen and circulatory flow. Type I collagen, fortified <i>via</i> cross-linking to prevent deformation and leaking during culture, was identified as a suitable gel wall material for supporting primary organoid-derived human duodenal epithelial cell attachment and monolayer formation. Addition of DAPT and PGE2 to culture medium paired with air-liquid interface culture increased the thickness of the mucus layer on epithelium grown within the device for 5 days from approximately 5 μm to 50 μm, making the model suitable for revealing intriguing features of interactions between luminal contents and the mucus barrier using live cell imaging. Time-lapse imaging of nanoparticle diffusion within mucus revealed a zone adjacent to the epithelium largely devoid of nanoparticles up to 4.5 h after introduction to the lumen channel, as well as pockets of dimly lectin-stained mucus within which particles freely diffused, and apparent clumping of particles by mucus components. Multiple particle tracking conducted on the intact mucus layer in the chip revealed significant size-dependent differences in measured diffusion coefficients. <i>E. coli</i> introduced to the lumen channel were freely mobile within the mucus layer and appeared to intermittently contact the epithelial surface over 30 minute periods of culture. Mucus shedding into the lumen and turnover of mucus components within cells were visualized. Taken together, this system represents a powerful tool for visualization of interactions between luminal contents and an intact live mucosal barrier.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid screening of CO2 capture fluids.","authors":"Yaohao Guo,Feng Li,Sepehr Saber,Mohammad Zargartalebi,Siyu Sonia Sun,Yurou Celine Xiao,Bo Bao,Zhi Xu,David Sinton","doi":"10.1039/d4lc00772g","DOIUrl":"https://doi.org/10.1039/d4lc00772g","url":null,"abstract":"The evaluation of CO2 capture fluids is crucial for the advancement of carbon capture technologies. Recent advancements in amine-based carbon capture fluids motivate a broad search for high-performance fluids and the development of methods capable of exploring a large chemical space. Here, we present a microfluidic approach paired with automated image processing and density functional theory simulations that enables comprehensive rapid screening of capture fluids. The principle of measurement leverages the ability to monitor phase expansion and contraction in fixed-volume dead-end channels. This approach enables fast comparative assessments of reaction kinetics and thermodynamic parameters, including CO2 absorption rate (∼30 s), desorption rate (∼30 s), absorption capacity (∼20 min), and vapor pressure (∼5 min), exceeding the speed of conventional methods by two orders of magnitude. The method is broadly applicable, effective for primary, secondary, and tertiary amine types. Rapid screening of capture fluids holds promise for the accelerated discovery of improved CO2 capture processes and an opportunity for the microfluidics community to contribute to decarbonization efforts.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"40 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144087765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-efficiency microfluidic chip integrated with micro-patterned planar spiral sensors for magnetic nanoparticle detection.","authors":"Nguyen Van Tuan,Ho Anh Tam,Nguyen Thi Ngoc,Vu Nguyen Thuc,Nguyen Khac Binh,Nguyen Thi Phuong Thao,Do Thi Hien,Bui Trong Sang,Nguyen Hoang Nam,Vu Dinh Lam,Le Van Lich,Do Thi Huong Giang","doi":"10.1039/d5lc00121h","DOIUrl":"https://doi.org/10.1039/d5lc00121h","url":null,"abstract":"Magnetic nanoparticles have garnered significant attention in the biomedical field due to their remarkable biocompatibility and diverse applications. However, existing methodologies for quantifying magnetic-labeled samples face limitations, particularly regarding the stringent requirements for magnetic sensors and the complexities associated with integrating these systems into microfluidic platforms. This study introduces an innovative planar magnetoimpedance sensor for magnetic nanoparticle detection, designed with a micropatterned spiral configuration and integrated into a microfluidic channel. The spiral configurations of the planar sensor are designed and optimized through micromagnetic simulations, where the domain properties of the sensors are examined by varying the turn widths of the spiral micropatterns from 70 μm to 210 μm. The optimal width is identified at 70 μm for effective measurement of magnetic particles. The magnetoimpedance sensor is fabricated using wet chemical etching based on an FeSiC ribbon. The computation-guided design of the magnetoimpedance sensor achieves impressive sensitivity and resolution values of 2.5% Oe-1 and 0.01 Oe, respectively. The designed sensor, integrated with the microfluidic channel, can detect magnetic nanoparticles as small as 0.2 μg. Both experiment and simulation results demonstrate that the magnetoimpedance effect is significantly influenced by the configurations of the transverse magnetic domain, resulting in detectable variation of the stray field in the MI sensor's output signals. Integrating the magnetoimpedance sensor with the microfluidic system provides several advantages, including cost-effectiveness, rapid response times, and user-friendliness. This quantitative detection method for magnetic nanoparticles holds substantial promise for applications in biological concentration detection and other advanced research domains.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"38 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144087786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Well-defined assembly of plasmonic metal nanoparticles by dielectrophoresis for highly sensitive SERS-active substrates.","authors":"Yun Su Yeo,Jaejun Park,Sunghoon Yoo,Dong Hwan Nam,Hayoung Kim,Tae Jae Lee,Gyu Leem,Jae-Sung Kwon,Seunghyun Lee","doi":"10.1039/d5lc00238a","DOIUrl":"https://doi.org/10.1039/d5lc00238a","url":null,"abstract":"In this study, dielectrophoresis (DEP) was performed to develop highly sensitive surface- enhanced Raman scattering (SERS)-active substrates for molecular sensing. Substrates with a circular hole pattern were used, and plasmonic particles were trapped and immobilized along the edges of the pattern using dielectrophoretic forces. The arranged particles created hotspots, resulting in an enhanced SERS signal that was detectable even at concentrations as low as 10-10 M. This uniform arrangement provided a consistent signal over a large area. In addition, it was experimentally verified that the behavior of the particles varied with pattern diameter. This phenomenon was further supported by theoretical analysis. The proposed DEP-based SERS substrates are expected to be useful in various applications due to their excellent reproducibility and reliability.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"57 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144065817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cancer-on-a-chip for precision cancer medicine.","authors":"Lunan Liu, Huishu Wang, Ruiqi Chen, Yujing Song, William Wei, David Baek, Mahan Gillin, Katsuo Kurabayashi, Weiqiang Chen","doi":"10.1039/d4lc01043d","DOIUrl":"10.1039/d4lc01043d","url":null,"abstract":"<p><p>Many cancer therapies fail in clinical trials despite showing potent efficacy in preclinical studies. One of the key reasons is the adopted preclinical models cannot recapitulate the complex tumor microenvironment (TME) and reflect the heterogeneity and patient specificity in human cancer. Cancer-on-a-chip (CoC) microphysiological systems can closely mimic the complex anatomical features and microenvironment interactions in an actual tumor, enabling more accurate disease modeling and therapy testing. This review article concisely summarizes and highlights the state-of-the-art progresses in CoC development for modeling critical TME compartments including the tumor vasculature, stromal and immune niche, as well as its applications in therapying screening. Current dilemma in cancer therapy development demonstrates that future preclinical models should reflect patient specific pathophysiology and heterogeneity with high accuracy and enable high-throughput screening for anticancer drug discovery and development. Therefore, CoC should be evolved as well. We explore future directions and discuss the pathway to develop the next generation of CoC models for precision cancer medicine, such as patient-derived chip, organoids-on-a-chip, and multi-organs-on-a-chip with high fidelity. We also discuss how the integration of sensors and microenvironmental control modules can provide a more comprehensive investigation of disease mechanisms and therapies. Next, we outline the roadmap of future standardization and translation of CoC technology toward real-world applications in pharmaceutical development and clinical settings for precision cancer medicine and the practical challenges and ethical concerns. Finally, we overview how applying advanced artificial intelligence tools and computational models could exploit CoC-derived data and augment the analytical ability of CoC.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082394/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A SAW-driven modular acoustofluidic tweezer.","authors":"Dachuan Sang, Suyu Ding, Qinran Wei, Fengmeng Teng, Haixiang Zheng, Yu Zhang, Dong Zhang, Xiasheng Guo","doi":"10.1039/d4lc00924j","DOIUrl":"https://doi.org/10.1039/d4lc00924j","url":null,"abstract":"<p><p>In surface acoustic wave (SAW)-driven acoustofluidic tweezers (AFTs), most setups are integrated on a piezoelectric substrate for a single purpose, limiting the reusability and versatility of devices fabricated using complex MEMS technologies. Meanwhile, prevalent devices exhibit anisotropy in SAW excitation and propagation, as well as optical birefringence and limited transmittance. This work presents a SAW-driven modular acoustofluidic tweezer consisting of up to four replaceable interdigital transducer (IDT) modules and a function module assembled on a common base. Since the IDT modules are separated, each can be fabricated using the piezoelectric substrate best suited to the requirements. For example, SAWs generated from different directions can simultaneously propagate along the <i>X</i>-axis of 128° <i>Y</i>-cut LiNbO₃, enabling highly efficient excitations. The generated SAWs couple into the function module with excellent optical properties and convert into Lamb waves, which then leak into the microfluidic domain and act on the fluid/particles. All modules are connected <i>via</i> standardized interfaces, eliminating potential instabilities caused by wired connections. The reliability of the setup is demonstrated <i>via</i> particle/cell patterning, separation, and concentration experiments, during which the replaceability and reusability of different modules, and the other advantages of the setup, <i>e.g.</i>, simple assembly, ease of operation, and application flexibility, are proven.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compartmentalized perfusion for temporal control of the chemical microenvironment of iPSC-derived cardiac cells.","authors":"Kaisa Tornberg,Wolfram Grötsch,Niina Ritari,Saara Haikka,Lassi Sukki,Katriina Aalto-Setälä,Mari Pekkanen-Mattila,Pasi Kallio","doi":"10.1039/d5lc00072f","DOIUrl":"https://doi.org/10.1039/d5lc00072f","url":null,"abstract":"Organ-on-chip structures are predicted to have a significant influence in drug research. In these structures, perfusion can provide cells a more controllable environment to receive signaling molecules. In many current organ-on-chip applications, perfusion is used for shear stress stimulus for the cells, but it can also provide a more precise way of controlling the chemical microenvironment around the cells. In this paper, we propose an open-top organ-on-chip structure with compartment-specific perfusion to introduce stimulating molecules to cells with only minimal extra unspecific stimulus. Using numerical simulations, we show that shear stress sensed by the cells within the structure is low. We further validated the flow profile experimentally. We showed that the hiPSC-CMs accommodate to the flow environment where the shear stress is kept below 0.035 mPa. We also show that the beating rate of hiPSC-CMs increases due to the stimulation provided by chemical stimulant molecules introduced through the flow.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"5 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143982519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Lab-on-a-Chip to Lab-on-a-Chip-in-the-Lab: a perspective of clinical laboratory medicine for the microtechnologist†","authors":"Kirby Fibben, Evelyn Kendall Williams, John D. Roback, Wilbur A. Lam and David N. Alter","doi":"10.1039/D4LC00614C","DOIUrl":"10.1039/D4LC00614C","url":null,"abstract":"<p >An overview of the evolving role of microfluidics within clinical laboratories and diagnostic settings. It explores how microfluidic technologies, initially envisioned to replace traditional lab practices, are instead integrating into established workflows. This integration is driven by advancements in miniaturization and automation, enhancing efficiency and expanding testing capabilities. Regulatory frameworks such as CLIA and FDA oversight shape the landscape for microfluidic adoption, emphasizing the need for rigorous validation and compliance. The total testing process (TTP) framework underscores the critical phases—pre-analytical, analytical, and post-analytical—where microfluidics must conform with to ensure accuracy and reliability in diagnostic outcomes. Automation emerges as pivotal by streamlining workflows and reducing errors, particularly in sample handling and result interpretation. Challenges persist including the complex categorization of tests and the push for tighter regulation of laboratory developed tests (LDTs). The challenges necessitate collaboration between researchers, clinicians, and regulatory bodies. This review highlights how automation and integration of microfluidic technologies in point-of-care settings are reshaping clinical diagnostics, offering rapid, personalized testing options while maintaining high standards of patient care. Despite advancements, mitigating diagnostic errors remains paramount, requiring continuous refinement of technologies and adherence to established clinical protocols. Ultimately, the successful integration of microfluidics into clinical laboratories hinges on balancing innovation with regulatory compliance, ensuring seamless usability and consistent diagnostic accuracy within existing healthcare infrastructures.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 11","pages":" 2566-2577"},"PeriodicalIF":6.1,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d4lc00614c?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143982437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}