Lab on a Chip最新文献

{"title":"Integrated Heating & Sensing for PCB EWOD Chips on a Digital Microfluidics Cloud Platform","authors":"Mosfera Chowdury, Gnanesh Nagesh, Hyun-Sung \"Eric\" Cho, Qining Leo Wang, Bhawya Bhawya, Abdulrahman Altabbaa, Lina Rose, Simon Rondeau-Gagné, Chang-Jin \"CJ\" Kim, Mohammed Jalal Ahamed","doi":"10.1039/d5lc00507h","DOIUrl":"https://doi.org/10.1039/d5lc00507h","url":null,"abstract":"Digital microfluidics (DMF) based on electrowetting-on-dielectric (EWOD) is a versatile platform that offers automated and precise droplet handling. Despite advances, one of the critical components—an integrated thermal module—remains underdeveloped, limiting the accuracy and reliability of bioassays. This paper presents a new thermal management module compatible with printed circuit board (PCB)-based DMF devices co-fabricated through standard PCB manufacturing. The module integrates a microheater and sensor pair interfaced with a closed-loop control system for individual droplet's temperature control. Numerical simulations were performed to understand heat transfer from the embedded microheaters to the droplets for optimized microheater design. Experiments were performed to evaluate key performance metrics of the module, including temperature accuracy, control stability, response time, crosstalk, and heat localization. Finally, a glucose assay was conducted on-chip to demonstrate the module’s applicability. The thermal module design presented in this paper offers seamless integration of thermal management for PCB-based DMF chips at little additional manufacturing cost while supporting the cloud-based platform established to democratize DMF.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"676 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144645705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Throughput Impedance Monitoring in 3D Tumor Cultures: A Multiplex, Microfluidic-Free Platform for Drug Screening","authors":"Attilio Marino, Kamil Ziaja, Marie Celine Lefevre, Maria Cristina Ceccarelli, Matteo Battaglini, Carlo Filippeschi, Gianni Ciofani","doi":"10.1039/d5lc00540j","DOIUrl":"https://doi.org/10.1039/d5lc00540j","url":null,"abstract":"The development of an effective therapy against glioblastoma (GBM) remains a significant and unmet clinical need. To address this challenge, creating predictive, physiologically relevant screening models is essential for accelerating the identification of promising drug candidates. In this paper, we present a novel impedance-based device where two-photon polymerization-fabricated scaffolds embedding electrodes are colonized by glioblastoma cells, effectively replicating the three-dimensional environment of the microscopic tumor foci that persist following tumor resection and cause recurrence. The results demonstrated that the proposed GBM-on-chip model enables high-throughput, multiplexed, and real-time monitoring of tumor spheroid development and their responses to therapeutic agents. Validation studies demonstrated the platform ability to detect subtle cytotoxic effects undetectable by traditional immunofluorescence methods, with optical transparency enabling complementary imaging analysis. This system represents a versatile framework for assessing drug efficacy in complex, physiologically relevant 3D tumor models, paving the way for innovations in cancer pharmacology.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"10 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144645253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bistable magnetic valves for selective sweat sampling in wearable microfluidics","authors":"Chaemin Kim, Chanyong Shin, Anna Lee, Jonghyun Ha, Jungil Choi","doi":"10.1039/d5lc00576k","DOIUrl":"https://doi.org/10.1039/d5lc00576k","url":null,"abstract":"Selective sweat sampling with high spatial and temporal resolution remains a key challenge in wearable microfluidic systems for biochemical monitoring. Here, we present a skin-conformal microfluidic platform that enables targeted, chamber-specific sweat collection by integrating bistable, magnetoactive elastomeric valves. Each valve is toggled between open and closed states using a simple external magnetic field, requiring no continuous power. The bistable design provides mechanical memory, maintaining valve states without sustained actuation, and thus allows highly energy-efficient fluid control. By embedding magnetic particles in a shell structure with geometric bistability, we achieve reliable magnetic actuation and characterize the critical pressures associated with valve switching under varying magnetic flux densities. These results demonstrate the feasibility of using the system for practical, localized sweat collection and suggest its utility in wearable sensing applications that require spatially discrete and contamination-free sampling.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"5 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144645706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid prototyping of multicompartment Liver-on-Chip for dynamic administration of tumour derived vesicles within an electrospun scaffold","authors":"Maria Testa, Marco Loria, Francesco Lopresti, Chiara Di Marco, Maïwenn Kersaudy-Kerhoas, Fabio Bucchieri, Marzia Pucci, Elisa Costanzo, Simone Scilabra, Riccardo Alessandro, Simona Fontana, V. La Carrubba","doi":"10.1039/d5lc00353a","DOIUrl":"https://doi.org/10.1039/d5lc00353a","url":null,"abstract":"A novel multicompartment Liver-on-Chip (LoC) platform has been developed combining laser-micromachined poly(methylmethacrylate) (PMMA) layers with an electrospun poly-lactic acid (PLA) scaffold to emulate the liver's extracellular matrix (ECM) for advanced in vitro modeling. The platform supports the dynamic, chronic delivery of colorectal cancer-derived extracellular vesicles (CRC-EVs) under physiologically relevant conditions. The use of thermoplastic materials such as PMMA provides advantages including low absorption, high optical clarity, and reproducibility, while the biomimetic architecture of the PLA scaffold enhances structural and functional fidelity. The LoC platform demonstrates significant advancements over conventional 2D cultures and static systems. Proteomic analyses revealed enhanced hepatocyte differentiation and activation of liver-specific pathways when cells were cultured on the PLA scaffold under both static and dynamic conditions. Dynamic CRC-EV administration induced the upregulation of the mesenchymal marker Vimentin in the hepatocytes, as previously described in the 2D system. This study establishes the LoC as a groundbreaking tool for investigating tumor-liver interactions and pre-metastatic niche formation. By addressing critical limitations of existing platforms, this system advances organ-on-chip technology for cancer research and therapeutic development.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"109 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144652374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of a bioreactor combining soft lithography and vat photopolymerisation to study tissues and multicellular organisms under dynamic culture conditions.","authors":"Thomas Meynard,Félix Royer,Robin Houssier,Orégane Bajeux,Sonia Paget,Fatima Lahdaoui,Alejandra Mogrovejo Valdivia,Nathalie Maubon,Jérôme Vicogne,Isabelle Van Seuningen,Vincent Senez","doi":"10.1039/d5lc00172b","DOIUrl":"https://doi.org/10.1039/d5lc00172b","url":null,"abstract":"Despite its capability to create much more realistic microenvironments for in vitro culturing of animal or human biological models, the spread of microfluidic tools in the world of biology and medicine has still not reached the predicted scale. Major obstacles to their widespread acceptance by end-users are manufacturing cost and operational complexity. 3D printing is a technology that is now widely democratised, thanks to its ease of use and very attractive cost/performance ratio. In particular, photopolymerisation through a liquid crystal screen is experiencing a very significant growth. Here, we describe the methodology we developed to evaluate this microfabrication technique and selected a photoprintable resin to manufacture a fluidic microsystem dedicated to tissue or micro-organism culture. The first originality of our approach lies in the architecture of the microsystem, which is made up of an elementary culture chamber in two parts, making it very easy to open after the culture period to carry out ex situ biochemical analyses. The second one is the nature of the materials used to make up the culture chamber, which consists of polydimethylsiloxane and a photoprinted resin. This hybrid assembly, combining an elastomer and a rigid plastic material, ensures a better seal and better dimensional control once the assembly is complete. We demonstrate the ability of our protocol to flexibly fabricate different culture chambers with dimensions down to 100 microns and we show for one of them three applications: a 2D layer of cell lines, a parasitic worm and a 3D microtissue from a pancreatic cancer patient.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"37 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144640306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D Modelling of Pulmonary Arterial Stenosis and Endothelial Dysfunction in CTEPH","authors":"Salina Nicoleau, Ylenia Roger Valle, Olga Tura-Ceide, Chloe Armour, Joan Albert Barbera, A. J. McKinnon Thomas, Deepa Gopalan, Beata Wojciak-Stothard","doi":"10.1039/d5lc00300h","DOIUrl":"https://doi.org/10.1039/d5lc00300h","url":null,"abstract":"Chronic thromboembolic pulmonary hypertension (CTEPH) arises from progressive thrombotic occlusion of pulmonary arteries, involving vessel blockage by unresolved thrombi and small vessel arteriopathy. This disrupts blood flow, increases lung pressure, and alters vessel geometry, contributing to endothelial dysfunction. However, the mechanisms remain unclear. To study these interactions, we developed microfluidic 3D models of pulmonary arteries with 30–80% stenosis using CTPAs from CTEPH and acute pulmonary embolism (APE) patients, in silico flow simulations, 3D printing, and soft lithography. Unlike standard circular channels, we designed semi-circular channels enclosed by a coverslip, which computational modelling confirmed closely mimics real vessel flow dynamics. Human pulmonary artery endothelial cells (HPAECs) cultured in 30–80% stenosis channels exhibited increased expression of pro-inflammatory, pro-thrombotic, and pro-angiogenic genes, with responses varying by stenosis severity and location. Cells in post-stenotic dilatation regions (60–80% stenosis) lost alignment and junctional integrity due to disturbed flow. The transcriptional profile of HPAECs from 80% stenosis channels closely resembled that of CTEPH pulmonary endarterectomy specimens. Platelet adhesion, dependent on von Willebrand factor (VWF), varied with stenosis severity and flow rate. Low perfusion rates increased adhesion in stenotic regions, while higher flow rates promoted adhesion post-stenosis. Our patient data-based stenosis models provide a robust platform for studying the effects of vascular geometry on blood flow, endothelial responses, and platelet aggregation, advancing research on CTEPH, pulmonary embolism, and other diseases associated with vascular occlusion.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"109 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144645254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Massively parallel photosensitive hydrogel encapsulated single-cell to a cluster of cells patterning and bone regeneration application","authors":"Sarin Abraham, Gayathri R, Kavitha Govarthanan, Suresh Ranga Rao, Moeto Nagai, Tuhin Subhra Santra","doi":"10.1039/d5lc00459d","DOIUrl":"https://doi.org/10.1039/d5lc00459d","url":null,"abstract":"Recent advances in cell biology and biomedical research have shifted from traditional two-dimensional (2D) to three-dimensional (3D) cell cultures. To aid in the study of various tissues, we present a high-throughput cell patterning device that encapsulates single-cells to a small cluster of cells within the hydrogels. The device with a 1cm x 1cm area, creates an array of 3D hydrogel-encapsulated micro-patterns ranging from 80µm × 80µm to 250µm × 250µm using photolithography, with gaps between the micro-patterns varying from 100µm to 200µm (center to center). At a concentration of 2 x 10⁷ cells/mL, the device demonstrated ~100% patterning efficiency by consistently accommodating ~13 cells per pattern for 250µm × 250µm micro-pattern array with an excellent cell viability of ~ 97.21%. 80µm × 80µm patterns also showed an efficiency of 94.4%, with ~ 5 cells per pattern. The probability of encapsulating a single-cells was ~ 54.23% for the 80µm × 80µm micro-pattern, however it decreased to 36.1% for 250µm × 250µm micro-pattern with a cell viability of ~95%. Compared to the existing literature, we achieved an improved probability of encapsulating a single-cell with a higher cell viability for 80µm x 80µm micro-pattern, with a 40% reduction in photoinitiator concentration incorporated with gelatin methacryloyl (GelMA) and a 60% decrease in exposure time. This massively parallel cell patterning technique was applied to five cell lines with highest cell viability of 97.2% for NIH-3T3 cells. Furthermore, we engineered the micro-pattern device to enhance patterned osteogenic differentiation by incorporating synthesized nano-hydroxyapatite (nHA) with GelMA by encapsulating MC3T3-E1 preosteoblast cells for bone regeneration applications. The results demonstrated significant increases in osteogenic differentiation with 1%(w/v) nHA treatment after 14 days which was validated through UV absorbance and RT-PCR. Thus, this platform enables comprehensive cellular research through cell-to-cell interaction studies, and patterned biomineralization processes, facilitating tissue architecture simulation and advancing biomedical research.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"109 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144629939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Teach your microscope how to print: low-cost and rapid-iteration microfabrication for biology","authors":"Lucien Hinderling, Remo Hadorn, Moritz Kwasny, Joël Frei, Benjamin Grädel, Sacha Psalmon, Yannick Blum, Rémi Berthoz, Alex E. Landolt, Benjamin D. Towbin, Daniel Riveline, Olivier Pertz","doi":"10.1039/d5lc00181a","DOIUrl":"https://doi.org/10.1039/d5lc00181a","url":null,"abstract":"The application of traditional microfabrication techniques to biological research is hindered by their reliance on clean rooms, expensive or toxic materials, and slow iteration cycles. We present an accessible microfabrication workflow that addresses these challenges by integrating consumer 3D printing techniques and repurposing standard fluorescence microscopes equipped with DMDs for maskless photolithography. Our method achieves micrometer-scale precision across centimeter-sized areas without clean room infrastructure, using affordable and readily available consumables. We demonstrate the versatility of this approach through four biological applications: inducing cytoskeletal protrusions <em>via</em> 1 μm-resolution surface topographies; micropatterning to standardize cell and tissue morphology; fabricating multilayer microfluidic devices for confined cell migration studies; imprinting agar chambers for long-time tracking of <em>C. elegans</em>. Our protocol drastically reduces material costs compared to conventional methods and enables design-to-device turnaround within a day. By leveraging open-source microscope control software and existing lab equipment, our workflow lowers the entry barrier to microfabrication, enabling labs to prototype custom solutions for diverse experimental needs while maintaining compatibility with soft lithography and downstream biological assays.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"21 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144622724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A urine detection chip for the analysis of urinary cells and extracellular vesicles for bladder cancer screening","authors":"Junyuan Liu, Yuxin Qu, Xun Xu, Zixing Ye, Jing Cheng, Han Wang","doi":"10.1039/d5lc00511f","DOIUrl":"https://doi.org/10.1039/d5lc00511f","url":null,"abstract":"Bladder cancer is the most prevalent malignant neoplasm of the urinary system. However, current techniques for bladder cancer diagnosis and prognosis are associated with significant patient discomfort and potential risk of urinary tract infection. To address these issues, we propose a non-invasive urine detection chip for isolating and identifying bladder cancer-related cells and extracellular vesicles. This chip enables simultaneous capture of exfoliated cells and blood cells for analysis, followed by microparticle-based filtration to remove cells and cell debris before capturing and analyzing extracellular vesicles for bladder cancer diagnosis. This method holds great potential in point-of-care screening and prognosis of bladder cancer.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"51 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144622726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic technologies for neuroblastoma","authors":"Leena Khelifa, Yubing Hu, Jennifer Tall, Rasha Khelifa, Amina Ali, Evon Poon, Mohamed Zaki Khelifa, Guowei Yang, Catarina Jones, Rosalia Moreddu, Nan Jiang, Savas Tasoglu, Louis Chesler and Ali K. Yetisen","doi":"10.1039/D4LC00005F","DOIUrl":"10.1039/D4LC00005F","url":null,"abstract":"<p >Neuroblastoma is an aggressive childhood cancer characterised by high relapse rates and heterogenicity. Current medical diagnostic methods involve an array of techniques, from blood tests to tumour biopsies. This process is associated with long-term physical and psychological trauma. Moreover, current technologies do not identify neuroblastoma at an early stage while tumours are easily resectable. In recent decades, many advancements have been made for neuroblastoma diagnosis, including liquid biopsy platforms, radiomics, artificial intelligence (AI) integration and biosensor technologies. These innovations support the trend towards rapid, non-invasive and cost-effective diagnostic methods which can be utilised for accurate risk stratification. Point-of-care (POC) diagnostic devices enable rapid and accurate detection of disease biomarkers and can be performed at the location of the patient. Whilst POC diagnostics has been well-researched within the oncological landscape, few devices have been reported for neuroblastoma, and these remain in the early research phase and as such are limited by lack of clinical validation. Recent research has revealed several potential biomarkers which have great translational potential for POC diagnosis, including proteomic, metabolic and epigenetic markers such as <em>MYCN</em> amplification and microRNAs (miRNAs). Using POC devices to detect high-risk biomarkers in biofluids such as blood and urine, offers a non-invasive approach to diagnosis of neuroblastoma, enabling early screening at a population level as well as real-time health monitoring at home. This is critical to mitigating long-term morbidity associated with late diagnosis and unnecessary treatment, in turn improving outcomes for neuroblastoma patients.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 15","pages":" 3630-3664"},"PeriodicalIF":6.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d4lc00005f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144622725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}