Lab on a Chip最新文献_第3页

An integrated microfluidic device for sorting of tumor organoids using image recognition†

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-12-04 DOI: 10.1039/D4LC00746H

Xingyang Yan, Deng Tan, Lei Yu, Danyu Li, Zhenghao Wang, Weiren Huang and Hongkai Wu

引用次数: 0

Rapid automated production of tubular 3D intestine-on-a-chip with diverse cell types using coaxial bioprinting†

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-12-03 DOI: 10.1039/D4LC00731J

Heeju Song, Yeonjin Hong and Hyungseok Lee

{"title":"Rapid automated production of tubular 3D intestine-on-a-chip with diverse cell types using coaxial bioprinting†","authors":"Heeju Song, Yeonjin Hong and Hyungseok Lee","doi":"10.1039/D4LC00731J","DOIUrl":"10.1039/D4LC00731J","url":null,"abstract":"Despite considerable animal sacrifices and investments, drug development often falters in clinical trials due to species differences. To address this issue, specific in vitro models, such as organ-on-a-chip technology using human cells in microfluidic devices, are recognized as promising alternatives. Among the various organs, the human small intestine plays a pivotal role in drug development, particularly in the assessment of digestion and nutrient absorption. However, current intestine-on-a-chip devices struggle to accurately replicate the complex 3D tubular structures of the human small intestine, particularly when it comes to integrating a variety of cell types effectively. This limitation is primarily due to conventional fabrication methods, such as soft lithography and replica molding. In this research, we introduce a novel coaxial bioprinting method to construct 3D tubular structures that closely emulate the organization and functionality of the small intestine with multiple cell types. To ensure stable production of these small intestine-like tubular structures, we analyzed the rheological properties of bioinks to select the most suitable materials for coaxial bioprinting technology. Additionally, we conducted biological assessments to validate the gene expression patterns and functional attributes of the 3D intestine-on-a-chip. Our 3D intestine-on-a-chip, which faithfully replicates intestinal functions and organization, demonstrates clear superiority in both structure and biological function compared to the conventional 2D model. This innovative approach holds significant promise for a wide range of future applications.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 1","pages":" 90-101"},"PeriodicalIF":6.1,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142793997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flow cell for high throughput Raman spectroscopy of non-transparent solutions†

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-11-29 DOI: 10.1039/D4LC00586D

Filippo Zorzi, Emil Alstrup Jensen, Murat Serhatlioglu, Silvio Bonfadini, Morten Hanefeld Dziegiel, Luigino Criante and Anders Kristensen

引用次数: 0

Data storage based on the absence of nucleotides using a bacteriophage abortive infection system reverse transcriptase. 利用噬菌体流产感染系统逆转录酶，根据核苷酸的缺失情况进行数据存储。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-11-28 DOI: 10.1039/d4lc00755g

Gregor Bajc, Anja Pavlin, Małgorzata Figiel, Weronika Zajko, Marcin Nowotny, Matej Butala

{"title":"Data storage based on the absence of nucleotides using a bacteriophage abortive infection system reverse transcriptase.","authors":"Gregor Bajc, Anja Pavlin, Małgorzata Figiel, Weronika Zajko, Marcin Nowotny, Matej Butala","doi":"10.1039/d4lc00755g","DOIUrl":"https://doi.org/10.1039/d4lc00755g","url":null,"abstract":"DNA molecules are a promising data storage medium for the future; however, effective de novo synthesis of DNA using an enzyme that catalyzes the polymerization of natural nucleoside triphosphates in a user-defined manner, without the need for multiple injections of polymerase, remains a challenge. In the present study, we demonstrated that the bacteriophage abortive infection system reverse transcriptase AbiK from Lactococcus lactis facilitates such an approach. We employed surface plasmon resonance to monitor the polymerization of the DNA strand with a user-defined sequence of multiple segments through a sequential buffer exchange process. Using this method, we synthesized synthetic DNA with segments of random length and a sequence consisting of only three of the four natural nucleotides. The information is encoded using the absence of one nucleotide in each segment. We demonstrated that synthetic DNA can be stored on the chip, and when the DNA is released from the chip, the second strand can be synthesized and read by sequencing. Our setup facilitates a writing speed of one nucleotide in less than 1 s and holds enormous potential for synthesizing DNA for data storage.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Controlled Au-coated PDMS microwell array for surface-enhanced DNA biochips†

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-11-27 DOI: 10.1039/D4LC00654B

Yeongseok Jang and Jonghyun Oh

{"title":"Controlled Au-coated PDMS microwell array for surface-enhanced DNA biochips†","authors":"Yeongseok Jang and Jonghyun Oh","doi":"10.1039/D4LC00654B","DOIUrl":"10.1039/D4LC00654B","url":null,"abstract":"Microwell technology is crucial in biological applications due to its ability to handle small sample sizes and perform numerous assays efficiently. This study aimed to develop a novel technique for microwell fabrication using pressure-assisted steam technology, offering lower cost, simplicity, and high reproducibility. Mechanical properties of microwell surfaces were successfully controlled and characterized, making them suitable for DNA capture. The application of gold coating generated an electric field within designed microwells, facilitating stable DNA detection. These microwells exhibited effective DNA sensing capabilities, validated using fluorescently stained lambda DNA at various concentrations (86, 8.6, and 0.86 ng μL−1). In particular, the 2.8 mm microwell showed a greater change in fluorescence intensity depending on DNA concentration than other microwells. At a concentration of 0.86 ng μL−1, to assess producibility using relative standard deviation (RSD) values as a DNA sensor, they were measured as 5.29, 2.76, and 1.85% for 1, 1.7, and 2.8 mm microwells, respectively. These results indicated that our proposed microwell exhibited efficient performance and good reproducibility. We believe that the developed method could be potentially used for high-throughput analysis as a biosensor for DNA applications.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 1","pages":" 79-89"},"PeriodicalIF":6.1,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142764711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A nanobody-based microfluidic chip for fast and automated purification of protein complexes† 基于纳米抗体的微流控芯片，用于快速自动纯化蛋白质复合物。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-11-26 DOI: 10.1039/D4LC00728J

Phebe De Keyser, Mitch de Waard, Ignaas S. M. Jimidar, Sandrien Verloy, Steven Janvier, Valentina Kalichuk, Thomas Zögg, Alexandre Wohlkönig, Els Pardon, Jan Steyaert and Gert Desmet

{"title":"A nanobody-based microfluidic chip for fast and automated purification of protein complexes†","authors":"Phebe De Keyser, Mitch de Waard, Ignaas S. M. Jimidar, Sandrien Verloy, Steven Janvier, Valentina Kalichuk, Thomas Zögg, Alexandre Wohlkönig, Els Pardon, Jan Steyaert and Gert Desmet","doi":"10.1039/D4LC00728J","DOIUrl":"10.1039/D4LC00728J","url":null,"abstract":"Many proteins, especially eukaryotic proteins, membrane proteins and protein complexes, are challenging to study because they are difficult to purify in their native state without disrupting the interactions with their partners. Hence, our lab developed a novel purification technique employing Nanobodies® (Nbs). This technique, called nanobody exchange chromatography (NANEX), utilises an immobilised low-affinity Nb to capture the target protein, which is subsequently eluted – along with its interaction partners – by introducing a high-affinity Nb. In line with the growing trend towards studying proteins in smaller sample sizes, the present study validates miniaturisation of NANEX in a packed bed microfluidic (μNANEX) chip. This μNANEX setup integrates up to five submicroliter silicon chips, enabling fully automated and reproducible purifications within minutes. Additionally, a digital twin model of the μNANEX column, which accurately predicts the effect of the reaction kinetics and mass transfer on the elution peaks, has been validated over a broad range of experimental conditions. The effectiveness of the method is demonstrated with Nbs binding to the green fluorescent protein (GFP), allowing streamlined purification of any GFP fusion protein from biological samples. Specifically, we used μNANEX to purify 0.1–1 μg of GFP-fused yeast proteins from 20 μL crude lysate and identified their interaction partners via mass spectrometry, showing that μNANEX purification preserves protein complexes.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 24","pages":" 5421-5432"},"PeriodicalIF":6.1,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142714862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Image-based fuzzy logic control for pressure-driven droplet microfluidics as autosampler for multimodal imaging microscopy. 基于图像的模糊逻辑控制，用于压力驱动液滴微流体作为多模态成像显微镜的自动进样器。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-11-26 DOI: 10.1039/d4lc00583j

Fabian Ott, Tobias Meyer-Zedler, Michael Schmitt, Jürgen Popp

引用次数: 0

Polydopamine-mediated gold nanoparticle coating strategy and its application in photothermal polymerase chain reaction. 聚多巴胺介导的金纳米粒子涂层策略及其在光热聚合酶链反应中的应用。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-11-26 DOI: 10.1039/d4lc00554f

Woo Ri Chae, Yoon-Jae Song, Nae Yoon Lee

{"title":"Polydopamine-mediated gold nanoparticle coating strategy and its application in photothermal polymerase chain reaction.","authors":"Woo Ri Chae, Yoon-Jae Song, Nae Yoon Lee","doi":"10.1039/d4lc00554f","DOIUrl":"10.1039/d4lc00554f","url":null,"abstract":"Materials with high light-to-heat conversion efficiencies offer valuable strategies for remote heating. These materials find wide applications in photothermal therapy, water distillation, and gene delivery. In this study, we investigated a universal coating method to impart photothermal features to various surfaces. Polydopamine, a well-known adhesive material inspired by mussels, served as an intermediate layer to anchor polyethyleneimine and capture gold nanoparticles. Subsequently, the coated surface underwent electroless gold deposition to improve photothermal heating efficiency by increasing light absorption. This process was analyzed through scanning electron microscopic imaging and absorbance measurements. To demonstrate functionality, the coated surface was photothermally heated using a light-emitting diode controlled with a microprocessor, targeting the metal regulatory transcription factor 1 gene-a marker for osteoarthritis-and the S gene of the severe fever with thrombocytopenia syndrome virus. Successful amplification of the target genes was confirmed after 34 polymerase chain reaction cycles in just 12 min, verified by gel electrophoresis, demonstrating its diagnostic applicability. Overall, this simple photothermal coating method provides versatile utility, and is applicable to diverse surfaces such as membranes, tissue culture dishes, and microfluidic systems.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142714865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revealing transport, uptake and damage of polystyrene microplastics using a gut-liver-on-a-chip. 利用肠肝芯片揭示聚苯乙烯微塑料的迁移、吸收和破坏。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-11-26 DOI: 10.1039/d4lc00578c

Yushen Wang, Junlei Han, Wenteng Tang, Xiaolong Zhang, Jiemeng Ding, Zhipeng Xu, Wei Song, Xinyu Li, Li Wang

{"title":"Revealing transport, uptake and damage of polystyrene microplastics using a gut-liver-on-a-chip.","authors":"Yushen Wang, Junlei Han, Wenteng Tang, Xiaolong Zhang, Jiemeng Ding, Zhipeng Xu, Wei Song, Xinyu Li, Li Wang","doi":"10.1039/d4lc00578c","DOIUrl":"10.1039/d4lc00578c","url":null,"abstract":"Microplastics (MPs) are pervasive pollutants present in various environments. They have the capability to infiltrate the human gastrointestinal tract through avenues like water and food, and ultimately accumulating within the liver. However, due to the absence of reliable platforms, the transportation, uptake, and damage of microplastics in the gut-liver axis remain unclear. Here, we present the development of a gut-liver-on-a-chip (GLOC) featuring biomimetic intestinal peristalsis and a dynamic hepatic flow environment, exploring the translocation in the intestines and accumulation in the liver of MPs following oral ingestion. In comparison to conventional co-culture platforms, this chip has the capability to mimic essential physical microenvironments found within the intestines and liver (e.g., intestinal peristalsis and liver blood flow). It effectively reproduces the physiological characteristics of the intestine and liver (e.g., intestinal barrier and liver metabolism). Moreover, we infused polyethylene MPs with a diameter of 100 nm into the intestinal and hepatic chambers (concentrations ranging from 0 to 1 mg mL-1). We observed that as intestinal peristalsis increased (0%, 1%, 3%, 5%), the transport rate of MPs decreased, while the levels of oxidative stress and damage in hepatic cells decreased correspondingly. Our GLOC elucidates the process of MP transport in the intestine and uptake in the liver following oral ingestion. It underscores the critical role of intestinal peristalsis in protecting the liver from damage, and provides a novel research platform for assessing the organ-specific effects of MPs.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142714867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Repeated pulses of ultrasound maintain sperm motility†

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-11-26 DOI: 10.1039/D4LC00826J

Ali Vafaie, Sahar Shahali, Mohammad Reza Raveshi, Reza Nosrati and Adrian Neild

引用次数: 0