Lab on a Chip最新文献_第3页

Analysis of Sperm Beating Characteristics Using Microfluidic Trapping and Machine-Learning-Based Flagellum Tracking 基于微流体捕获和机器学习的鞭毛跟踪的精子跳动特性分析

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-09-20 DOI: 10.1039/d5lc00389j

Aisha Hamidu, Omar Abdelgawad, Ahmed Azmeer, Mohamed Abdelgawad

{"title":"Analysis of Sperm Beating Characteristics Using Microfluidic Trapping and Machine-Learning-Based Flagellum Tracking","authors":"Aisha Hamidu, Omar Abdelgawad, Ahmed Azmeer, Mohamed Abdelgawad","doi":"10.1039/d5lc00389j","DOIUrl":"https://doi.org/10.1039/d5lc00389j","url":null,"abstract":"Male infertility affects a significant portion of couples worldwide, with standard semen analysis often failing to identify functional deficiencies in sperm performance. This study presents a microfluidic platform for characterizing sperm flagellar beating patterns with unprecedented detail, providing insights into sperm functional parameters potentially linked to unexplained infertility. We combined microcontact printing of fibronectin adhesion spots with machine-learning-based flagellum tracking to immobilize sperm heads while allowing free flagellar movement, enabling precise analysis of beating characteristics. Our tracking algorithm utilizes YOLOv8 (You Only Look Once) machine learning-based computer vision model and which we trained using 750 manually annotated images of sperm cells. We used keypoint detection along the sperm flagellum to calculate critical beating parameters including the beating amplitude, frequency, and asymmetry patterns. To validate the platform, we investigated the effects of established capacitation and hyperactivation agents on sperm motility. Caffeine treatment (10-40 mM) increased flagellar beat amplitude by up to 65% while decreasing frequency by approximately 50%, with pronounced asymmetrical beating consistent with hyperactivation. Heparin exposure (10-100 μg/ml) similarly enhanced beating amplitude by approximately 25% without significantly altering frequency. We also analysed the beating pattern of sperm cells immobilized inside a microchannel under different flow velocities. Results revealed a decrease in the beating frequency when sperm cells were subjected to flow. The platform eliminates the need for sophisticated sperm tracking techniques which facilitates high-throughput analysis under controlled physicochemical conditions. By enabling detailed characterization of sperm flagellar behaviour under various stimuli, our platform offers a valuable tool for investigating molecular mechanisms underlying idiopathic male infertility and evaluating potential therapeutic interventions.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"17 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145089586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: A nanobody-based microfluidic chip for fast and automated purification of protein complexes 更正：一种基于纳米体的微流控芯片，用于快速和自动纯化蛋白质复合物

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-09-19 DOI: 10.1039/d5lc90098k

Phebe De Keyser, Mitch de Waard, Ignaas S. M. Jimidar, Sandrien Verloy, Steven Janvier, Valentina Kalichuk, Thomas Zögg, Alexandre Wohlkönig, Els Pardon, Jan Steyaert, Gert Desmet

引用次数: 0

Microfluidic Single-Cell Drug Screening: Toward Personalized Precision Therapy in Chronic Myeloid Leukemia 微流控单细胞药物筛选：迈向慢性髓性白血病个体化精准治疗

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-09-18 DOI: 10.1039/d5lc00779h

Hwisu Jeon, Yukyung Park, Soo-Hyun Kim, Chang-Yeol Jung, Hongtae Kim, Eujin Um, Dong-Wook Kim, Taesung Kim

{"title":"Microfluidic Single-Cell Drug Screening: Toward Personalized Precision Therapy in Chronic Myeloid Leukemia","authors":"Hwisu Jeon, Yukyung Park, Soo-Hyun Kim, Chang-Yeol Jung, Hongtae Kim, Eujin Um, Dong-Wook Kim, Taesung Kim","doi":"10.1039/d5lc00779h","DOIUrl":"https://doi.org/10.1039/d5lc00779h","url":null,"abstract":"Chronic myeloid leukemia (CML) treatment employs several FDA-approved BCR::ABL1 tyrosine kinase inhibitors (TKIs) with distinct efficacy and side effects influenced by patient-specific factors. This study introduces a microfluidic cell culture array for the comparative analysis of six BCR::ABL1 TKIs, namely imatinib, nilotinib, bosutinib, ponatinib, dasatinib, and asciminib, using CML-related cell lines. The device provides a continuous, chemostat-like microfluidic environment that enables quantitative drug sensitivity scoring. The microchambers for cell culture notably offer advantages for single-cell imaging of suspension leukemia cells, which tend to aggregate in conventional culture platforms. This system supports the detailed characterization of cell viability across various TKI types and concentrations, yielding comprehensive mathematical metrics to assess relative drug efficacy. In this study, we compared drug responses in K562 and Ba/F3 BCR::ABL1 cell lines, including T315I mutant variant, and specifically demonstrated that Ba/F3 cells harboring the T315I mutation exhibit resistance to the first- and second-generation TKIs, responding only to ponatinib and asciminib. We further validated the device with a CML patient-derived bone marrow sample, requiring only minimal adjustments to the experimental conditions. The proposed microfluidic single-cell-based screening array could refine treatment regimens and advance personalized medicine in CML.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145077679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel holder and microfluidic system for spatially controlled hypoxia induction, mechanical stimulation and cardiac regeneration research 一种用于空间控制缺氧诱导、机械刺激和心脏再生研究的新型支架和微流体系统

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-09-18 DOI: 10.1039/d5lc00460h

Kołodziejek Dominik, Szlachetka Aleksandra, Iwoń-Szczawińska Zuzanna, Drozd Marcin, Wojasiński Michał, Jastrzębska Elżbieta

{"title":"A novel holder and microfluidic system for spatially controlled hypoxia induction, mechanical stimulation and cardiac regeneration research","authors":"Kołodziejek Dominik, Szlachetka Aleksandra, Iwoń-Szczawińska Zuzanna, Drozd Marcin, Wojasiński Michał, Jastrzębska Elżbieta","doi":"10.1039/d5lc00460h","DOIUrl":"https://doi.org/10.1039/d5lc00460h","url":null,"abstract":"Over the years, cardiovascular diseases have remained a leading cause of mortality worldwide, necessitating advanced experimental models to mimic the natural environment of the heart as closely as possible. New microfluidic heart models would enable precise modelling of the healthy heart as well as pathological mechanisms occurring during cardiac ischemia and testing new therapies would become more accessible than it is currently. In this study, we developed a two layer holder for creating hypoxic conditions in a dedicated microfluidic system for modelling cardiac infarction. By integrating the holder with a microsystem utilizing nanofibrous mats with iron(II, III) oxide (Fe3O4) magnetic nanoparticles we were able to create a tool for using (potentially patient specific) induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) in cardiac research. In the microsystem we induced hypoxia and conducted preliminary observations of cardiac regeneration using induced pluripotent stem cells (iPSCs). We found that iPSCs express cTnT when co-cultured with hypoxia-damaged iPSC-CMs indicating that the holder with an integrated microsystem can be used for hypoxia and cardiac regeneration studies.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"76 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145077683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Open-Space Microfluidics as a Tool to Study Signaling Dynamics 开放空间微流体作为研究信号动力学的工具

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-09-18 DOI: 10.1039/d5lc00521c

Maude Proulx, Pierre Clapperton-Richard, Alisa Piekny, Laurent Potvin-Trottier, Thomas Gervais

{"title":"Open-Space Microfluidics as a Tool to Study Signaling Dynamics","authors":"Maude Proulx, Pierre Clapperton-Richard, Alisa Piekny, Laurent Potvin-Trottier, Thomas Gervais","doi":"10.1039/d5lc00521c","DOIUrl":"https://doi.org/10.1039/d5lc00521c","url":null,"abstract":"The temporal dynamics of cell signaling are a crucial way for cells to regulate their transcriptional targets and consequently may heavily influence cell responses. Improving our understanding of signaling dynamics is important for drug treatments targeting specific signaling pathways. However, studying signaling dynamics requires multiplexed, time-sensitive experiments. Here, we use an open-space microfluidic device, the microfluidic display, which enables liquid delivery from above a surface, forming defined and stable confinement zones without enclosing samples into a chip. A device with rapid reagent switching (<7 seconds) and 6 independent confinement areas is first designed. Using this platform, we study the Notch pathway in engineered C2C12 cells to display constitutively active Notch receptors upon which we force highly controlled time-dependent modulation patterns by delivering time-varying doses of the Notch inhibitor DAPT. We replicate previous findings on Notch activation with our methodology by confirming the Notch-regulated gene Hes1 is upregulated for short Notch activation pulses, while Hey1 required sustained activation. We confirm a previously observed regime switch from Hes1 to Hey1 dominance between 2h and 3h of activation. Finally, by varying signal pulses while keeping dose constant in six independent experiments performed simultaneously, we further show the upregulation of the Hes1 gene for multiple short pulses, while Hey1 activation depends on duty cycle length. These results highlight microfluidic displays as a valuable tool for systems biology, enabling multiplexed, high temporal resolution stimulation of signaling pathways.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"221 4 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145077684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Barcode-Integrated Cellulose Based Microfluidic System for Intelligent Point-of-Care Blood Typing 基于纤维素的条形码集成微流体系统，用于智能护理点血型

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-09-18 DOI: 10.1039/d5lc00688k

Chunrui Chen, Tonghuan Zhan, Lin Hu, Mengyuan Ding, Xianchang Wu, Heng Wang, Bing Xu

{"title":"Barcode-Integrated Cellulose Based Microfluidic System for Intelligent Point-of-Care Blood Typing","authors":"Chunrui Chen, Tonghuan Zhan, Lin Hu, Mengyuan Ding, Xianchang Wu, Heng Wang, Bing Xu","doi":"10.1039/d5lc00688k","DOIUrl":"https://doi.org/10.1039/d5lc00688k","url":null,"abstract":"Accurate and rapid blood typing plays a critical role in life-saving clinical procedures such as blood transfusions and organ transplantation. Herein, we proposed a novel blood typing system (BloodStrips) that combines cellulose based microfluidics with universal barcode technology, achieving intelligent, rapid, and user-friendly blood type detection. The BloodStrips system employed heat transfer printing to create barcode patterns on hydrophobic cotton substrates, and integrated cotton threads to construct hydrophilic channels. Meanwhile, the swinging elution method was harnessed to remove free red blood cells (RBCs) while retaining aggregated RBCs on the cotton threads, thereby resulting in creating a distinct white/red contrast at the macro level (white represents cotton thread, red represents bloodstain). The white/red barcodes with different combinations was used to represent various blood types. Based on this principle, we further developed a portable and automated blood tying chip called BloodBar chip. Noting that this device leverages simple and straightforward smartphone scanning technique to decipher blood types, avoiding reading errors caused by ambient light intensity and personal bias. This work provides a universal and intelligent visual diagnostic platform for simple, rapid, and accurate blood typing, which may find wide applications in developing countries or resource-limited areas.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"82 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145077686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stress-actuated Partially Flexible Microelectrode Arrays for Activity Recording in 3D Neuronal Cultures 用于三维神经元培养活动记录的应力驱动部分柔性微电极阵列

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-09-17 DOI: 10.1039/d4lc01077a

João Serra, José C Mateus, Susana Cardoso, João Ventura, Paulo Aguiar, Diana C Leitao

{"title":"Stress-actuated Partially Flexible Microelectrode Arrays for Activity Recording in 3D Neuronal Cultures","authors":"João Serra, José C Mateus, Susana Cardoso, João Ventura, Paulo Aguiar, Diana C Leitao","doi":"10.1039/d4lc01077a","DOIUrl":"https://doi.org/10.1039/d4lc01077a","url":null,"abstract":"Microelectrode arrays (MEAs) are instrumental in monitoring electrogenic cell populations, such as neuronal cultures, allowing high precision measurements of electrical activity. Although three-dimensional neuronal cultures replicate the behavior of in vivo systems better than two-dimensional models, conventional planar MEAs are not well suited to capture activity within such networks. Novel MEA geometries can overcome this difficulty, but often at the cost of increased fabrication complexity. Here, we used the stress mismatch between thin film layers to fabricate MEAs with vertical electrodes, using methods compatible with established microfabrication protocols. A micrometric SiO2 hinge enables control over the bending angle of flexible polyimide structures with embedded electrodes. The performance of the patterned electrodes was assessed before and after stress actuation, through impedance measurements, voltage noise mapping, and neuronal activity recordings. 3D MEAs with 30 × 30 μm2 electrodes showed an impedance of 0.96 ± 0.07 MΩ per electrode and detected neuronal activity spikes with amplitudes as high as 400 μV. These results demonstrate the potential of the developed methods to provide a scalable approach to fabricate 3D MEAs, enabling enhanced recording capabilities for in vitro neuronal cultures.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"104 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145072319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A biocompatible surfactant film for stable microfluidic droplets 用于稳定微流体液滴的生物相容性表面活性剂膜

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-09-17 DOI: 10.1039/d5lc00456j

Brendan Deveney, John A Heyman, Raoul G. Rosenthal, David A. Weitz, Jörg G. Werner

引用次数: 0

Optimizing Microfluidic Flow Cell Geometry for In-Situ Resonant Soft X-ray Characterization of Molecular Nanostructures 优化微流体流动池几何结构用于分子纳米结构的原位共振软x射线表征

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-09-16 DOI: 10.1039/d5lc00765h

Devin Grabner, Terry McAfee, Cheng Wang, Matthew Marcus, Brian Collins

{"title":"Optimizing Microfluidic Flow Cell Geometry for In-Situ Resonant Soft X-ray Characterization of Molecular Nanostructures","authors":"Devin Grabner, Terry McAfee, Cheng Wang, Matthew Marcus, Brian Collins","doi":"10.1039/d5lc00765h","DOIUrl":"https://doi.org/10.1039/d5lc00765h","url":null,"abstract":"Liquid-phase resonant soft X-ray scattering (LP-RSoXS) is an emerging label-free technique to probe chemically resolved nanostructures of molecular or hybrid materials in liquid environments. Still, quantitative analysis is hindered by the pressure-induced deformation of thin silicon nitride (SiN) membranes used as windows in microfluidic flow cells, which attenuates the signal in nonlinear ways, making experimental optimization difficult. Here, we directly characterize this deformation under experimental conditions for a variety of cell configurations. We use this to develop a predictive model that combines transmission effects of SiN bowing, incident X-ray beam profiles, and material-dependent resonant scattering cross sections to simulate the effective scattering intensity at the detector across the carbon K-edge. Maps of the total signal across the flow cell window reveal that increasing the window width and polymer concentration shifts the anisotropic intensity distributions from the center toward the edges of the window. It was determined that an optimal SiN thickness of 50 nm, with a window aperture of 104 µm, maximizes the total signal for typical solute concentrations and energies across the carbon K-edge. Our results overturn the assumption that corner regions dominate the scattering signal, offering explicit design guidelines for maximizing LP-RSoXS signals and significantly advancing the quantitative application of this technique to the characterization of molecular and hybrid nanostructured materials in liquids.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"26 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145072321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Candyfluidics: The Art of Fabricating Micro-and Nano-fluidic Geometries using Surface-deposited Sugar Scaffolds 糖流体：利用表面沉积的糖支架制造微和纳米流体几何形状的艺术

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-09-16 DOI: 10.1039/d5lc00710k

Tochukwu D Anyaduba, J. Rodriguez-Manzano

{"title":"Candyfluidics: The Art of Fabricating Micro-and Nano-fluidic Geometries using Surface-deposited Sugar Scaffolds","authors":"Tochukwu D Anyaduba, J. Rodriguez-Manzano","doi":"10.1039/d5lc00710k","DOIUrl":"https://doi.org/10.1039/d5lc00710k","url":null,"abstract":"The adoption of miniaturization technologies, such as micro and nanofluidic systems, as a strategy for democratized healthcare in developing countries was proposed decades ago. However, regions in most need of cost-effective global health technological solutions such as sub-Saharan Africa, contribute less than 1% of the global research output in the microfluidics field. This dearth of research output from the region may be attributed to economic and technical barriers to rapid prototyping with state-of-the-art tools such as 3D printing and micromilling. As a more accessible, low-cost, and low-resource alternative, we introduce candyfluidics, an innovative method for fabricating micro-and nano-fluidic structures using sugar and screenprinting, which are respectively abundant materials and technology in the region. Exemplified through the creation of flow-focusing chips, we provide detailed procedure for creating microfluidic architectures from surface-deposited candy mixture. The resulting flow-focusing chips were validated by generating water-in-oil droplets with volumes ranging from 0.2 to 1.22 nL under pressure-driven flows. Furthermore, the chips were used to demonstrate digital droplet loop-mediated isothermal amplification for the detection of dengue virus type 1 nucleic acids at femtomolar concentration (∼ 85 copies/ µL). The Candyfluidics fabrication process takes less than 30 minutes and enables the parallel production of multiple chips, offering a rapid and scalable approach to manufacturing microfluidic devices for point-of-need applications.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"649 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145068083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0