用于稳定微流体液滴的生物相容性表面活性剂膜

IF 5.4 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS
Lab on a Chip Pub Date : 2025-09-17 DOI:10.1039/d5lc00456j
Brendan Deveney, John A Heyman, Raoul G. Rosenthal, David A. Weitz, Jörg G. Werner
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引用次数: 0

摘要

由于液滴的体积小,易于生产,因此液滴可以作为分析单个生物物种(如核酸和单细胞)的实用隔间。然而,液滴之间的聚结是一个持续存在的挑战,通常阻碍了基于液滴技术的应用,特别是在液滴受到恶劣条件或必须长时间保持稳定的情况下。在这里,我们介绍了一种多功能成膜表面活性剂,可以形成稳定的液滴。薄膜是通过氟化相的定制聚合物和水相的含二醇大分子之间的共价相互作用在液滴界面上形成的。该膜可以在聚合酶链反应(PCR)中稳定液滴,并且具有生物相容性。这种表面活性剂为采用无规共聚物和界面缔合的新型表面活性剂化学提供了一个原型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A biocompatible surfactant film for stable microfluidic droplets
Droplets serve as practical compartments for the analysis of individual biological species like nucleic acids and single cells due to the small size and ease of production of droplets. However, coalescence among droplets is a persistent challenge that often precludes the application of droplet-based techniques, particularly in cases when droplets are subject to harsh conditions or must remain stable for extended periods of time. Here, we introduce a versatile film-forming surfactant that forms robustly stable droplets. The film is formed at the droplet interface through covalent interactions between a custom polymer in a fluorinated phase and a diol-containing macromolecule in an aqueous phase. The film can stabilize droplets during polymerase chain reaction (PCR) and is biocompatible. The surfactant provides an archetype for new surfactant chemistries employing random copolymers and interfacial association.
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来源期刊
Lab on a Chip
Lab on a Chip 工程技术-化学综合
CiteScore
11.10
自引率
8.20%
发文量
434
审稿时长
2.6 months
期刊介绍: Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.
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