Lab on a Chip最新文献_第4页

Integrated technologies for molecular profiling of genetic and modified biomarkers in extracellular vesicles. 细胞外囊泡中遗传和修饰生物标志物分子图谱的集成技术。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-03-26 DOI: 10.1039/d5lc00053j

Li Zhang, Chi Yan Wong, Huilin Shao

{"title":"Integrated technologies for molecular profiling of genetic and modified biomarkers in extracellular vesicles.","authors":"Li Zhang, Chi Yan Wong, Huilin Shao","doi":"10.1039/d5lc00053j","DOIUrl":"https://doi.org/10.1039/d5lc00053j","url":null,"abstract":"Extracellular vesicles (EVs) are nanoscale membrane vesicles actively released by cells into a variety of biofluids. EVs carry myriad molecular cargoes; these include classical genetic biomarkers inherited from the parent cells as well as EV modifications by other entities (e.g., small molecule drugs). Aided by these diverse cargoes, EVs enable long-distance intercellular communication and have been directly implicated in various disease pathologies. As such, EVs are being increasingly recognized as a source of valuable biomarkers for minimally-invasive disease diagnostics and prognostics. Despite the clinical potential, EV molecular profiling remains challenging, especially in clinical settings. Due to the nanoscale dimension of EVs as well as the abundance of contaminants in biofluids, conventional EV detection methods have limited resolution, require extensive sample processing and can lose rare biomarkers. To address these challenges, new micro- and nanotechnologies have been developed to discover EV biomarkers and empower clinical applications. In this review, we introduce EV biogenesis for different cargo incorporation, and discuss the use of various EV biomarkers for clinical applications. We also assess different chip-based integrated technologies developed to measure genetic and modified biomarkers in EVs. Finally, we highlight future opportunities in technology development to facilitate the clinical translation of various EV biomarkers.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visualization investigation of fluid transport in multiscale porous media for CO2-EOR based on microfluidic technology† 基于微流体技术的CO2-EOR多尺度多孔介质流体输运可视化研究

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-03-25 DOI: 10.1039/D5LC00019J

Jianxiang Wang, Jiafeng Sun, Jiawei Shi and Bo Bao

{"title":"Visualization investigation of fluid transport in multiscale porous media for CO2-EOR based on microfluidic technology†","authors":"Jianxiang Wang, Jiafeng Sun, Jiawei Shi and Bo Bao","doi":"10.1039/D5LC00019J","DOIUrl":"10.1039/D5LC00019J","url":null,"abstract":"During oil extraction, the recovery rates of traditional methods have been gradually declining. CO2-enhanced oil recovery (CO2-EOR) has been utilized since the 1960s; however, in recent years, it has garnered renewed attention due to its environmental benefits and economic advantages. However, there are few reports addressing multiphase mass transfer in micro- and nano-scale pores. This study employs microfluidic technology to simulate the pore structures of real reservoir rocks. A fracture–matrix porous medium chip with a network channel structure and a microscale porous medium chip featuring multiple pore-throat ratios were designed to investigate the effects of cross-scale interactions, network channel geometries, and the Jamin effect on fluid flow patterns and oil recovery rates during both CO2 miscible and CO2 immiscible flooding processes. The experiments demonstrated that the cross-scale effect facilitates the rapid achievement of a 100% recovery rate during CO2 miscible flooding, but exacerbates gas channeling during CO2 immiscible flooding, resulting in a decreased recovery rate. The Jamin effect becomes more pronounced with increasing pore-throat ratios, and the substantial capillary resistance generated by this effect in regions with high pore-throat ratios significantly reduces the rate of increase in recovery during CO2 miscible flooding, as well as the overall recovery rate during CO2 immiscible flooding. This study enhances the understanding of multiphase mass transfer in reservoir conditions and provides critical insights for optimizing CO2-EOR strategies, ultimately contributing to more efficient oil recovery and supporting sustainable practices in the energy sector.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 8","pages":" 1981-1992"},"PeriodicalIF":6.1,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MagSity platform: a hybrid magnetic levitation-based lensless holographic microscope platform for liquid density and viscosity measurements. 磁力平台：一种基于混合磁悬浮的无透镜全息显微镜平台，用于液体密度和粘度测量。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-03-24 DOI: 10.1039/d5lc00144g

Oyku Doyran Ince, H Cumhur Tekin

{"title":"MagSity platform: a hybrid magnetic levitation-based lensless holographic microscope platform for liquid density and viscosity measurements.","authors":"Oyku Doyran Ince, H Cumhur Tekin","doi":"10.1039/d5lc00144g","DOIUrl":"https://doi.org/10.1039/d5lc00144g","url":null,"abstract":"The viscosity and density of liquids are the most extensively studied material properties, as their accurate measurement is critical in various industries. Although developments in micro-viscometers have overcome the limitations of traditional bulky methods, more accessible technologies are required. Here, we introduce a novel magnetic levitation-based method to measure the viscosity and density of solutions in a microcapillary channel. This principle exploits microparticles as microsensors to correlate levitation time and height with solutions' viscosity and density, using buoyancy and drag forces. The platform has an integrated lensless holographic microscope, providing a hybrid system for in situ and precise measurements. By utilizing this hybrid technology, portable, rapid and cost-effective measurements can be conducted. This platform enables viscosity and density measurements within 7 minutes, achieving high accuracies of at least 97.7% and 99.9%, respectively, across an operation range of 0.84-5.09 cP and 1.00-1.09 g cm-3. The platform is utilized to clearly distinguish differences in the spent cell culture medium across various cell lines. This method, as presented, can be readily applied to measure a diverse array of liquids in multiple domains, encompassing biotechnology, medicine, and engineering.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of phospholipid vesicles using an electrokinetic turbulent microreactor 用电动湍流微反应器合成磷脂囊泡。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-03-21 DOI: 10.1039/D4LC00992D

Liangying Han, Yueqiang Zhu, Jin'an Pang, Xuejing Wang, Shenghua Ma, Xiaojun Han, Kaige Wang and Wei Zhao

{"title":"Synthesis of phospholipid vesicles using an electrokinetic turbulent microreactor","authors":"Liangying Han, Yueqiang Zhu, Jin'an Pang, Xuejing Wang, Shenghua Ma, Xiaojun Han, Kaige Wang and Wei Zhao","doi":"10.1039/D4LC00992D","DOIUrl":"10.1039/D4LC00992D","url":null,"abstract":"Microfluidic-based material synthesis is uniquely suited for the fabrication of reproducible and controllable products due to the highly controlled reaction environments in microscale dimensions. With many passive and active micromixers emerging for the on-chip material synthesis needs, the use of electrokinetic driven fluid to form turbulence actuation is yet an unexplored technique with much-unrealized potential. In this study, we used an electrokinetic turbulent micromixer for the controllable synthesis of phospholipid vesicles by nanoprecipitation. By imposing a transverse electric field upon coflowing reagent-containing solvent and antisolvent streams, the two fluids experience rapid mixing with bilayer lipid fragments generated. Phospholipid vesicles are facilitated under the effect of the electric field and turbulent flow. Depending on the voltage of the AC electric field, concentrations and types of phospholipids, and flow parameters, phospholipid vesicles of different sizes and morphologies can be synthesized in a single microfluidic chip, rather than a complex batch process in traditional methods. The time is no more than even a single second. The method is compatible for integration into a platform to produce phospholipid vesicles for chemistry and biomedical applications.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 8","pages":" 1959-1967"},"PeriodicalIF":6.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Luffa cylindrica-inspired powerless micropump: long-term, high-flow operation and energy-generation application† 丝瓜启发的无动力微泵：长期，高流量运行和发电应用。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-03-20 DOI: 10.1039/D5LC00068H

Jungjae Woo, Jeongmin Seo, Hyewon Cho, Soeun Park, Changsoo Han and Hyejeong Kim

{"title":"Luffa cylindrica-inspired powerless micropump: long-term, high-flow operation and energy-generation application†","authors":"Jungjae Woo, Jeongmin Seo, Hyewon Cho, Soeun Park, Changsoo Han and Hyejeong Kim","doi":"10.1039/D5LC00068H","DOIUrl":"10.1039/D5LC00068H","url":null,"abstract":"Powerless micropumps are in increasing demand for applications requiring portability, simplicity, and long-term operation. However, several existing passive pumps have limitations such as sustained high flow rates and extended operational periods. Inspired by the unique structural characteristics of Luffa cylindrica, this study aims to develop a biomimetic micropump capable of long-term and high-flow operation. By examining the water transport mechanisms in a hierarchical porous structure, we designed and fabricated micropumps that replicate these mechanisms. A key aspect of this design is the integration of flow resistors, which enables precise control over the absorption rates and extend the pumping duration. The cone-shaped agarose aerogel (AAG) micropump operates for over 930 min with an average flow rate of 5.6 μl min−1, demonstrating significant longevity. The agarose superabsorbent polymer aerogel (ASAG) micropump, while having a shorter operational duration of approximately 620 min, exhibited a significantly higher average pumping rate of 13.2 μl min−1. This study highlights the potential of bio-inspired designs for advancing efficient and powerless pumping systems. The proposed micropump shows promise for applications in microfluidic devices and reverse electrodialysis systems, where continuous and sustainable fluid transport is essential.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 8","pages":" 1968-1980"},"PeriodicalIF":6.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143661643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic mimicry of the Golgi-linked N-glycosylation machinery† 高尔基链n -糖基化机制的微流体模拟。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-03-20 DOI: 10.1039/D5LC00005J

Florin N. Isenrich, Marie-Estelle Losfeld, Markus Aebi and Andrew J. deMello

{"title":"Microfluidic mimicry of the Golgi-linked N-glycosylation machinery†","authors":"Florin N. Isenrich, Marie-Estelle Losfeld, Markus Aebi and Andrew J. deMello","doi":"10.1039/D5LC00005J","DOIUrl":"10.1039/D5LC00005J","url":null,"abstract":"The complexity of the eukaryotic glycosylation machinery hinders the development of cell-free protein glycosylation since in vitro methods struggle to simulate the natural environment of the glycosylation machinery. Microfluidic technologies have the potential to address this limitation due to their ability to control glycosylation parameters, such as enzyme/substrate concentrations and fluxes, in a rapid and precise manner. However, due to the complexity and sensitivity of the numerous components of the glycosylation machinery, very few “glycobiology-on-a-chip” systems have been proposed or reported in the literature. Herein, we describe the design, fabrication and proof-of-concept of a droplet-based microfluidic platform able to mimic N-linked glycan processing along the secretory pathway. Within a single microfluidic device, glycoproteins and glycosylation enzymes are encapsulated and incubated in water-in-oil droplets. Additional glycosylation enzymes are subsequently supplied to these droplets via picoinjection, allowing further glycoprotein processing in a user-defined manner. After system validation, the platform is used to perform two spatiotemporally separated consecutive enzymatic N-glycan modifications, mirroring the transition between the endoplasmic reticulum and early Golgi.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 8","pages":" 1907-1917"},"PeriodicalIF":6.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d5lc00005j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elevated interstitial flow in the cerebrospinal fluid microenvironment accelerates glioblastoma cell migration on a microfluidic chip† 微流控芯片上脑脊液微环境间质流量升高加速胶质母细胞瘤细胞迁移。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-03-20 DOI: 10.1039/D5LC00015G

Wanting Hu, Hua Sun, Huibo Qi, Linkai Jiang, Kaining Zhang, Xiaomeng Jia, Yu Wang, Yu Xiang and Qionglin Liang

{"title":"Elevated interstitial flow in the cerebrospinal fluid microenvironment accelerates glioblastoma cell migration on a microfluidic chip†","authors":"Wanting Hu, Hua Sun, Huibo Qi, Linkai Jiang, Kaining Zhang, Xiaomeng Jia, Yu Wang, Yu Xiang and Qionglin Liang","doi":"10.1039/D5LC00015G","DOIUrl":"10.1039/D5LC00015G","url":null,"abstract":"Glioblastoma is one of the most malignant tumors in the world, but the development of its therapies remains limited. Herein, a microfluidic chip that mimics the cerebrospinal fluid (CSF) circulation microenvironment is proposed to study the migration characteristics of glioblastoma U87-MG cells and U251 cells in complex environments where glioblastoma coexists with diseases that elevate CSF levels. In the presence of interstitial flow (IF), changing both cell densities and the cellular environment results in increased cell motility, including an increase in the number of migrating cells, the mean displacement of the top 30% fastest-moving cells, and the overall mean displacement. Then, through dynamic migration characterization analysis, it was found that IF enhances cell velocity and speed. Importantly, cells exposed to IF tend to migrate in directions with smaller angles of deviation from the opposite direction of IF. Finally, cytoskeleton inhibitors and decreased expressions of focal adhesion proteins, such as cytochalasin D, FAK inhibitors (VS-6063 and PF-573228), and FAK siRNA, were both proved to decrease the cells' response to IF. This work not only demonstrates the effect of IF on glioblastoma cell migration, but also indicates the reliability of microfluidic chips for modeling complex physiological environments, which is expected to be further developed for drug screening.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 8","pages":" 2085-2097"},"PeriodicalIF":6.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143661645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and development of a modular centrifugal platform with adjustable mixing and automated position-switching for stepwise gradient elution in reversed-phase liquid chromatography† 模块化离心平台的设计和开发，具有可调节的混合和自动位置切换，用于反相液相色谱的逐步梯度洗脱。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-03-18 DOI: 10.1039/D4LC01038H

Chia-Tse Shih and Chih-Hsin Shih

{"title":"Design and development of a modular centrifugal platform with adjustable mixing and automated position-switching for stepwise gradient elution in reversed-phase liquid chromatography†","authors":"Chia-Tse Shih and Chih-Hsin Shih","doi":"10.1039/D4LC01038H","DOIUrl":"10.1039/D4LC01038H","url":null,"abstract":"This study introduces a modular centrifugal platform developed for stepwise gradient elution in reversed-phase liquid chromatography, featuring adjustable mixing and automated position-switching mechanisms. Traditional methods of gradient elution rely on precision syringes and mixers to control eluent composition, but incorporating external pumping systems into centrifugal platforms presents substantial technical and economic challenges. To overcome these limitations, an adjustable eluent mixer was designed to generate concentration gradients by utilizing Coriolis-induced metering and shake-mode mixing processes. The eluent composition was controlled by varying the platform's rotational speed, with the effects of geometric and operational parameters on liquid distribution thoroughly analyzed. An operating curve was established to correlate methanol–water eluent compositions with rotational speed. Furthermore, a switchable fraction collector capable of automated position switching was developed to collect eluates from each elution step. By synchronizing rotational speed with ratchet-driven movements, the outer ring containing multiple fraction collectors rotates relative to the inner disk, enabling efficient replacement of filled collectors. Experimental results demonstrated the successful separation and collection of water-soluble dyes, highlighting the platform's potential as a cost-effective and precise solution for chromatographic applications.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 8","pages":" 2098-2108"},"PeriodicalIF":6.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A biosensor-integrated filtration device for nanoparticle isolation and label-free imaging† 一种用于纳米颗粒分离和无标记成像的生物传感器集成过滤装置。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-03-14 DOI: 10.1039/D5LC00089K

Leyang Liu, Takhmina Ayupova, Saurabh Umrao, Lucas D. Akin, Han-Keun Lee, Joseph Tibbs, Xing Wang, Utkan Demirci and Brian T. Cunningham

{"title":"A biosensor-integrated filtration device for nanoparticle isolation and label-free imaging†","authors":"Leyang Liu, Takhmina Ayupova, Saurabh Umrao, Lucas D. Akin, Han-Keun Lee, Joseph Tibbs, Xing Wang, Utkan Demirci and Brian T. Cunningham","doi":"10.1039/D5LC00089K","DOIUrl":"10.1039/D5LC00089K","url":null,"abstract":"Rapid, efficient, simple approaches for biological nanoparticle recovery from bodily fluids are required for translating detection strategies from lab diagnostics to low-resource settings, where expensive sample processing instruments such as an ultracentrifuge are not accessible. In this work, we characterize an alternative approach in which intact nanoparticles are filtered from plasma with a nanoporous filtration device that separates particulates within a 100–200 nm diameter range followed by detection on a photonic crystal (PC) biosensor with a portable photonic resonator interferometric scattering microscopy (PRISM) instrument. The biosensor-integrated recovery device's (BIRD) collection efficiency is initially characterized using gold nanoparticles and fluorescent nanobeads suspended in buffer solution and plasma, followed by spiking intact HIV pseudovirus into the same media. We demonstrate a recovery rate of 55.0% for 100 nm diameter AuNP and HIV spiked into the buffer and 11.9% for 100 nm diameter FluoSpheres spiked in human plasma. Using PRISM, we observed the Brownian motion of filtered nanoparticles and virions eluted into the detection compartment, with concentration-dependent counting of transient contact events between the nanoparticles and the PC surface.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 8","pages":" 2073-2084"},"PeriodicalIF":6.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11921766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intraluminal pressure triggers a rapid and persistent reinforcement of endothelial barriers† 腔内压力触发内皮屏障快速和持续的强化。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-03-13 DOI: 10.1039/D5LC00104H

Aurélien Bancaud, Tadaaki Nakajima, Jun-Ichi Suehiro, Baptiste Alric, Florent Morfoisse, Jean Cacheux and Yukiko T. Matsunaga

{"title":"Intraluminal pressure triggers a rapid and persistent reinforcement of endothelial barriers†","authors":"Aurélien Bancaud, Tadaaki Nakajima, Jun-Ichi Suehiro, Baptiste Alric, Florent Morfoisse, Jean Cacheux and Yukiko T. Matsunaga","doi":"10.1039/D5LC00104H","DOIUrl":"10.1039/D5LC00104H","url":null,"abstract":"In response to mechanical cues, endothelial cells elicit highly sensitive cellular response pathways that contribute to the regulation of the physiology and disorders of the vascular system. However, it remains relatively unexplored how endothelial tissues process and integrate the intraluminal pressure, and in turn regulate the permeation flow across the vessel wall. Leveraging a tissue engineering approach to create microvessels (MVs), we measured real-time permeation flow induced by intraluminal pressures ranging from 0.1 to 2.0 kPa. Our findings reveal that mechanically stimulated MVs strengthen their barrier function within seconds of exposure to pressures below 1 kPa, with this enhanced barrier function persisting for 30 minutes. We demonstrate that this barrier reinforcement is linked to the closure of paracellular gaps. Additionally, we observe that it is associated with, and depends on, actin cytoskeleton reorganization, including the accumulation of stress fibers near intercellular junctions and the broadening of adherence junction protein localization. These findings provide insights into the ability of endothelial tissues to regulate interstitial fluid flow in response to sudden increases in blood pressure.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 8","pages":" 2061-2072"},"PeriodicalIF":6.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d5lc00104h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0