Lab on a Chip最新文献

{"title":"Hybrid GC Platform: A Micro Gas Chromatography System with a Simple Configuration for Low-Concentration VOCs Analysis","authors":"Yeongseok Lee, Sangkyun Lee, Woojin Jang, Junwoo Lee, Yuntaek Choi, Si-Hyung Lim","doi":"10.1039/d5lc00268k","DOIUrl":"https://doi.org/10.1039/d5lc00268k","url":null,"abstract":"A compact hybrid gas chromatography (GC) platform was developed by integrating a previously reported hybrid µ-GC column chip (hybrid chip) and a commercial photoionization detector. The hybrid chip enabled both gas preconcentration and separation in a single device, allowing for a highly compact and simple platform design with a volume of 0.62 L. With a sample volume of 40.8 mL and an analysis time of 20 minutes, it achieved detection limits of 19.3, 22.8, 30.1, and 24.4 ppb for benzene, toluene, ethylbenzene, and ortho-xylene, respectively. The linear ranges were 0.25–1 ppm for benzene and toluene, 0.25–1.5 ppm for ethylbenzene, and 0.25–2 ppm for ortho-xylene. The peak capacity ranged from 5.34 to 8.81, with full width at half height between 0.22 and 0.5 min. Importantly, the detection limit for benzene was below US workplace air concentration limits set by the American Conference on Governmental Industrial Hygienists (ACGIH) and National Institute for Occupational Safety and Health (NIOSH), demonstrating the platform’s potential for indoor air monitoring. Furthermore, portability was enhanced through the integration with a battery and carrier gas filter pack. The platform consumed 2.65 W during analysis (20 minutes), and assuming one cycle consists of 20 minutes of analysis and 10 minutes of stand-by operation, the system could theoretically operate for 70 cycles (35 hours) on a single charge. Field testing with classroom and laboratory air samples confirmed the potential applicability of the platform. In addition, partial qualitative separations were achieved for alkanes, alcohols, aldehydes, and ketones, suggesting broader utility in fields beyond indoor air monitoring.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"45 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144252665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Point-of-care analysis for foodborne pathogens in food samples based on a fully enclosed microfluidic chip cartridge","authors":"Dayun Tang, Peng Li, Shuo Qi, Qiong Wu, Ruili Yu, Mei Liu, Zhouping Wang","doi":"10.1039/d4lc00819g","DOIUrl":"https://doi.org/10.1039/d4lc00819g","url":null,"abstract":"Foodborne pathogens endanger public health and rapid, sensitive, and accurate detection of them is vital. Portable, highly integrated detection devices have great application prospects in the screening and analysis of foodborne pathogens. In this study, a fully automated detection device based on a fully enclosed microfluidic chip cartridge was successfully designed. This device integrates multiple functions, including nucleic acid extraction, reagent preparation, LAMP reaction, and signal detection. By simply adding a sample, it can simultaneously detect four types of foodborne pathogens, making it advantageous for the analysis of complex samples and improving detection accuracy. Additionally, freeze-dried reagents are integrated into the fully enclosed microfluidic chip cartridge, which allows the reagents to be transported and stored at room temperature, greatly reducing the cost of detection. It has been successfully applied in actual samples contaminated with multiple foodborne pathogens and has excellent stability. The entire detection process can be completed in 45 minutes, with a sensitivity of approximately 500 CFU mL<small>⁻¹</small>. Therefore, the automated microfluidic device would be adequate for point-of-care testing (POCT) with high simplicity and high speed, providing an advanced genetic analysis microsystem for foodborne pathogen detection.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"135 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144252108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parallel DLD Microfluidics for Chloroplast Isolation and Sorting","authors":"Oriana Gerallin Chavez-Pineda, Pablo E. Guevara-Pantoja, Victor Marin, Gabriel Arturo Caballero-Robledo, Luis David Patino-Lopez, Daniel A May-Arrioja, Clelia De-la-Peña, Jose L. Garcia-Cordero","doi":"10.1039/d5lc00348b","DOIUrl":"https://doi.org/10.1039/d5lc00348b","url":null,"abstract":"Chloroplasts are characteristic organelles of plant cells, essential for photosynthesis and various other metabolic processes, including amino acid, lipid, and hormone biosynthesis. Beyond their classical functions, chloroplasts have emerged as promising targets in biotechnology, particularly in therapeutic applications and biofuel production. However, their isolation remains technically challenging due to the limitations of conventional methods, which typically require complex protocols, specialized equipment, and trained personnel. Here, we present a microfluidic-based platform that enables size-based chloroplast separation using deterministic lateral displacement (DLD). Our device integrates four parallel DLD arrays, each with a distinct critical diameter (CD). This configuration enables bandpass filtering and allows the simultaneous isolation of chloroplasts of various sizes within a single device. Shared inlets and uniform flow conditions across all arrays enhance reproducibility compared to conventional techniques. Unlike traditional sucrose density gradients, which lack precise size-based separation, our system achieves separation efficiencies of 50-85% for chloroplasts ranging from 3 to 8 µm, with recovered fractions having purities of 17-66%. This platform provides a rapid, automated, and scalable solution for chloroplast isolation, with significant potential applications in plant research, biotechnology, and synthetic biology.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"45 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144237778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An <i>in vivo</i> mimetic liver-lobule-chip (LLoC) for stem cell maturation, and zonation of hepatocyte-like cells on chip.","authors":"Philip Dalsbecker, Siiri Suominen, Muhammad Asim Faridi, Reza Mahdavi, Julia Johansson, Charlotte Hamngren Blomqvist, Mattias Goksör, Katriina Aalto-Setälä, Leena E Viiri, Caroline B Adiels","doi":"10.1039/d4lc00509k","DOIUrl":"https://doi.org/10.1039/d4lc00509k","url":null,"abstract":"<p><p><i>In vitro</i> cell culture models play a crucial role in preclinical drug discovery. To achieve optimal culturing environments and establish physiologically relevant organ-specific conditions, it is imperative to replicate <i>in vivo</i> scenarios when working with primary or induced pluripotent cell types. However, current approaches to recreating <i>in vivo</i> conditions and generating relevant 3D cell cultures still fall short. In this study, we validate a liver-lobule-chip (LLoC) containing 21 artificial liver lobules, each representing the smallest functional unit of the human liver. The LLoC facilitates diffusion-based perfusion <i>via</i> sinusoid-mimetic structures, providing physiologically relevant shear stress exposure and radial nutrient concentration gradients within each lobule. We demonstrate the feasibility of long term cultures (up to 14 days) of viable and functional HepG2 cells in a 3D discoid tissue structure, serving as initial proof of concept. Thereafter, we successfully differentiate sensitive, human induced pluripotent stem cell (iPSC)-derived cells into hepatocyte-like cells over a period of 20 days on-chip, exhibiting advancements in maturity compared to traditional 2D cultures. Further, hepatocyte-like cells cultured in the LLoC exhibit zonated protein expression profiles, indicating the presence of metabolic gradients characteristic of liver lobules. Our results highlight the suitability of the LLoC for long-term discoid tissue cultures, specifically for iPSCs, and their differentiation in a perfused environment. We envision the LLoC as a starting point for more advanced <i>in vitro</i> models, allowing for the combination of multiple liver cell types to create a comprehensive liver model for disease-onchip studies. Ultimately, when combined with stem cell technology, the LLoC offers a promising and robust on-chip liver model that serves as a viable alternative to primary hepatocyte cultures-ideally suited for preclinical drug screening and personalized medicine applications.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144245375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multi-channel chip enabled synchronized reciprocating-flow of fluid for rapid, simultaneous, multiplex detection of inflammatory markers.","authors":"Juanhua Li, Zihan Xiao, Tianyu Wu, Yiren Liu, Wenyong Zhang, Cuiping Zhou, Yanqiong Su, Hongrui Liang, Donglin Cao, Jianhua Zhou","doi":"10.1039/d5lc00132c","DOIUrl":"https://doi.org/10.1039/d5lc00132c","url":null,"abstract":"<p><p>Infections are common in daily life and can lead to various acute diseases with complex symptoms. Rapid and accurate detection of multiple inflammatory markers using multi-channel microfluidic chips provides important guidance for controlling the development of infections. However, current fluid control methods for multi-channel microfluidic chips typically involve controlling the liquid in each channel separately and successively, which increases the complexity of control operations and the detection time. Here, we proposed a circularly-aligned parallel channel chip (CAPC chip) with multiple channels and used this chip for the rapid, simultaneous detection of four inflammatory markers. We implemented the integrated fluid control method and achieved a synchronized reciprocating-flow of fluid and the precise control of the flow velocity of fluid in multiple channels using a single air-source pressure control device. Then, we used the CAPC chip to perform rapid, simultaneous enzyme-linked immunosorbent assay (ELISA) detection of four inflammatory markers (CRP, PCT, IL-6, SAA). The results showed that the CAPC chip can complete qualitative and quantitative detection of these four inflammatory markers within 5 min, which is the fastest ELISA detection for simultaneous detection of multiple protein biomarkers so far. Furthermore, we used the CAPC chip to detect these four inflammatory markers in simulated serum samples and provide prompt information regarding the classification and severity of infection. These findings demonstrate the potential of the CAPC chip for rapid, simultaneous, multiplex immunoassays.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144245374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Microfluidic Device for Passive Separation of Platelet-Rich Plasma from Whole Blood","authors":"Pablo E. Guevara-Pantoja, Yara Alvarez-Brana, Jon Mercader Ruiz, Fernando Benito-Lopez, Lourdes Basabe-Desmonts","doi":"10.1039/d5lc00362h","DOIUrl":"https://doi.org/10.1039/d5lc00362h","url":null,"abstract":"We present a microfluidic device for separating Platelet-Rich Plasma (PRP) from whole blood, addressing key limitations in current technologies. Unlike existing methods that require complex fabrication and expensive materials, our approach uses a CO2 laser cutter to fabricate acrylic layers bonded with pressure-sensitive adhesives, making it cost-effective and simple. Operating via gravity sedimentation, the device captures blood cells in multiple trenches and processes 1 mL of whole blood—significantly more than previous sedimentation devices. To prevent bubble formation, we incorporated a hydrophilic surface at the trench bottoms, ensuring reliable PRP separation. We analyzed three trench geometries to optimize plasma yield and quality. Our device processes blood in 45 minutes, yielding ~300 µL of plasma with at least a 2-fold platelet concentration increase. Red and white blood cell removal purities are 98% and 96%, respectively. Flow simulations optimized shear rates to improve sedimentation. Only 8.2% of the total platelets were activated, compared to 31% in the centrifugation method. This combination of simplicity, cost-efficiency, and effective platelet preservation enables a low-cost method for obtaining high-quality PRP for clinical research and therapy.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144228823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Size-selective sorting of kaolinite micro/nanoflakes via microfluidic filtration for wound hemostasis","authors":"Guangyao Li, Liang Wan, Ying Chen, Xuming Zhang, Aidong Tang, Huaming Yang","doi":"10.1039/d5lc00274e","DOIUrl":"https://doi.org/10.1039/d5lc00274e","url":null,"abstract":"Kaolinite, a natural micro/nano clay material, exhibits remarkable effect on wound hemostasis, yet its efficacy is critically limited by heterogeneous particle sizes. Therefore, sorting based on size differences is essential to improve its performance. However, kaolinite with layer structure presents challenges in sorting compared to spherical or elliptical materials, and the size distribution ranges continuously from nanometers to micrometers, which poses significant challenges for precise sorting. Hence, we developed a dual-layer microfluidic filtration chip, to enable high-throughput sorting of kaolinite micro/nanoflakes (size from 1.582 to 0.377 μm). The dual-layer filter membrane structure with graded pore sizes enabled selective sorting of kaolinite particles within a specific size range, and the co-flow fluid arrangement was employed to alleviate membrane clogging. The hemostatic properties of kaolinite particles with different sizes were evaluated through in-vivo and in-vitro experiments, revealing the significant size-dependent effects of kaolinite on wound hemostasis. The mechanism of different sizes of kaolinite in the process of coagulation, especially the effect on platelet activation and coagulation factor activation, provided theoretical basis for optimizing kaolinite-based hemostatic materials. This work established a scalable microfluidic strategy for precise sorting of sheet nanomaterials and improved the translational potential of kaolinite in emergency wound hemostasis.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"254 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144228826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A three-dimensional microfluidic device embedded within a thermal cycler tube for electrokinetic DNA extraction.","authors":"Qi Jiang, Xuehao Zang, Yilu Wang, Alexandre S Avaro, Diego A Huyke, Juan G Santiago","doi":"10.1039/d4lc01062k","DOIUrl":"https://doi.org/10.1039/d4lc01062k","url":null,"abstract":"<p><p>Microfluidic devices have been widely used in modern chemical and biological analyses as stand-alone units, typically in series with other equipment such as extraction columns, manual or robotic pipetting, and even advanced next-generation sequencing systems. While microfluidic devices have enhanced various aspects of laboratory workflows, their integration with established commercial assay platforms remains limited. To this end, we developed a three-dimensional microfluidic insert embedded directly into a commercially available polymerase chain reaction (PCR) tube. This integration creates a microfluidic device compatible with conventional thermal cyclers, which support complex temperature cycling and multiplexed fluorescence detection. The integrated system facilitates key bioassay functions like nucleic acid purification through a selective ionic focusing method known as isotachophoresis (ITP), PCR amplification, and real-time fluorescence detection. We validated the performance of the integrated system by purifying nucleic acids from raw human serum samples and detecting exogenous SARS-CoV-2 N gene using FAM-labeled TaqMan probes, with both the DNA extraction and detection carried out within the same PCR tube. We achieved a detection sensitivity of 100 cp μL^-1 within a total process time of 60 min in these experiments. Human serum samples processed without purification show no PCR amplification results. This integrated system demonstrates the powerful concept of integrating microfluidic structures into form factors compatible with the highly complex and sensitive operation of current off-the-shelf systems.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acoustic microstreaming and augmentation of gas exchange using an oscillating membrane towards microfluidic artificial lungs.","authors":"Anthony Mercader, Sung Kwon Cho","doi":"10.1039/d5lc00109a","DOIUrl":"https://doi.org/10.1039/d5lc00109a","url":null,"abstract":"<p><p>This paper presents a novel configuration for generating acoustic microstreaming flows at audible frequencies within a microchannel utilizing a pinned oscillating membrane. The characterization and interactions of these acoustic streaming flows with the streamwise flow within the microchannel are investigated, along with their effects on gas exchange augmentation. Advanced characterization methods and computational fluid dynamics simulations show a similar pattern and magnitude in acoustic streaming, providing evidence that this flexural membrane oscillation is the driving mechanism of the time-averaged vortices. This method exhibits potential application to microfluidic artificial lungs, particularly due to the vertical orientation of the resulting mixing, which facilitates an augmentation of gas exchange across the permeable membrane. Furthermore, it eliminates any obstructions in the microchannel and ensures stability, as opposed to other acoustic streaming methods such as sharp edge and oscillating bubble methods. Successful augmentation of gas exchange by up to 3.7× is demonstrated as shown by characterization of CO₂ transferred into the channel. Scaling up of throughput is also demonstrated with a branching design, featuring a multilayer manifold to avoid undesirable interaction of the streaming flow with the channel geometry.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-throughput enrichment of functional disulfide-rich peptides by droplet microfluidics","authors":"Jing Xie, Kuok Yap, Simon J. de Veer, Selvakumar Edwardraja, Thomas Durek, Matt Trau, David J. Craik, Conan K. Wang","doi":"10.1039/d5lc00261c","DOIUrl":"https://doi.org/10.1039/d5lc00261c","url":null,"abstract":"Disulfide-rich peptides (DRPs) have evolved intricate topologies to carry out a wide range of bioactivities throughout nature, e.g., in fungi, insects, plants and animals, and have proven applications in medicine and agriculture. To discover novel DRPs, it is now routine to screen DRP libraries for target affinity, but target binding does not necessarily correlate with function. This study reports an innovative platform for screening of DRP libraries based on the functional endpoint of biochemical reactions within picoliter-sized water-in-oil droplets. We leveraged yeast secretory expression to ensure proper assembly of disulfide connectivity, and thus peptide shape, and engineered customizable strains for facile detection of function (i.e., protease inhibitory activity) for libraries of DRPs. Rapid enrichment of a potent trypsin inhibitor (MCoTI-II) from a &gt;100,000 pool of randomized variants across four rounds of selection was achieved, far exceeding the library sizes explored previously for peptide systems in droplet microfluidics. This developed platform provides a foundation to explore the functional engineering of DRPs.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"13 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144211173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}