Lab on a Chip最新文献

{"title":"Correction: The effect of cyclic fluid perfusion on the proinflammatory tissue environment in osteoarthritis using equine joint-on-a-chip models","authors":"Johannes Heidenberger, Eva I. Reihs, Jonathan Strauss, Martin Frauenlob, Sinan Gültekin, Iris Gerner, Stefan Toegel, Peter Ertl, Reinhard Windhager, Florien Jenner and Mario Rothbauer","doi":"10.1039/D5LC90048D","DOIUrl":"10.1039/D5LC90048D","url":null,"abstract":"<p >Correction for ‘The effect of cyclic fluid perfusion on the proinflammatory tissue environment in osteoarthritis using equine joint-on-a-chip models’ by Johannes Heidenberger <em>et al.</em>, <em>Lab Chip</em>, 2025, <strong>25</strong>, 2256–2269, https://doi.org/10.1039/d4lc01078g.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 11","pages":" 2795-2796"},"PeriodicalIF":6.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d5lc90048d?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sample-sparing multiplexed antibody Fc biomarker discovery using a reconfigurable integrated microfluidic platform.","authors":"Hanhao Zhang,Divya Bhakta,Anushka Saha,Sai Preetham Peddireddy,Shumin Bao,Lei Li,Sukwan Handali,W Evan Secor,Lucia A O Fraga,Jessica K Fairley,Aniruddh Sarkar","doi":"10.1039/d5lc00042d","DOIUrl":"https://doi.org/10.1039/d5lc00042d","url":null,"abstract":"Control of endemic infectious diseases is often impeded by the lack of sensitive and specific yet easy-to-obtain biomarkers. Antibody fragment crystallizable (Fc) regions, such as Fc glycosylation, which are modulated in a pathogen-specific and disease-state-specific manner have emerged as potential such biomarkers. However current methods to perform large-scale antigen-specific antibody Fc feature screening for biomarker discovery often require too much sample volume, cost and expertise to be realistically realizable in many disease contexts. Here we present a simple, flexible and reconfigurable microfluidic device, made using rapid prototyping techniques, that can perform highly multiplexed and high-throughput biomarker discovery targeting both antibody fragment antigen-binding (Fab) and Fc features including antigen specificity, antibody isotypes, subclasses, N-glycosylation and Fc receptor binding. Using integration of an antigen microarray and reconfigurable microfluidics for sample and probe distribution, the device can perform a total of 1400 assays measuring 100 antibody Fab and Fc features per sample from a low sample volume (15 μL). The device demonstrates cleanroom-free simple fabrication and ease of use comparable to standard immunoassay platforms. Performance comparable to existing methods was validated and a biomarker screening for schistosomiasis, a helminth-mediated infection, was performed using clinical samples where antibody subclass-based biomarkers were successfully identified distinguishing current infection from former infection and endemic controls.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"23 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insect wing circulation: transient perfusion through a microfluidic dragonfly forewing model.","authors":"Sangjin Ryu,Haipeng Zhang,Tomer Palmon,Mary K Salcedo,Günther Pass,John J Socha","doi":"10.1039/d4lc00714j","DOIUrl":"https://doi.org/10.1039/d4lc00714j","url":null,"abstract":"Insect wings are made of an acellular composite material that forms a thin membrane and a complex network of veins. Veins are tubes that not only serve to stabilize the wing membrane, but they are also filled with hemolymph (insect blood). Thus, veins supply the sensory organs located on the wing veins with water and nutrients and enable the removal of waste products. In addition, the composite material of the wing is permanently hydrated by the hemolymph and thus retains the necessary flexibility. Hemolymph flow through the microfluidic vein network is therefore crucial for the functionality of insect wings. However, how perfusion occurs throughout the vein network in relation to hemolymph circulation is poorly understood. To investigate the dynamics of transient perfusion in complex wing venation, we developed a microfluidic wing vein model of the forewing of the common green darner dragonfly, Anax junius, using polydimethylsiloxane. Transient perfusion was simulated by injecting dye into the model filled with clear water; inversely, clear water was injected into dye in a separate trial. Visualized perfusion patterns suggest that the perfused portion of the vein network logarithmically increased with respect to time, which could be explained by a theoretical model of a simplified wing vein network, and that time differences occurred between the arrival of a new substance and the complete removal of an old substance in veins. Our biomimetic wing vein device enables further investigation into the unique circulatory system and transport phenomena of the insect wing. Also, our microfluidics-based approach proves a potential use of microfluidics in entomology and related fields.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"24 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143915015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a high-performance sliding microneedle-lateral flow immunoassay strip device for ultra-rapid point-of-care diagnosis†","authors":"Soo-bin Yu and Jae Hwan Jung","doi":"10.1039/D5LC00199D","DOIUrl":"10.1039/D5LC00199D","url":null,"abstract":"<p >Interstitial fluid (ISF) is a promising biofluid for non-invasive diagnostics, but its clinical application is limited by slow extraction rates and small sample volumes. To address these challenges, we developed the high-performance sliding microneedle-lateral flow immunoassay (HP-SML) device, an improved microneedle (MN)-based platform for rapid ISF extraction and on-site biomarker detection. The device incorporates extended MNs (1400 μm), a 3D-printed insertion case, and a lateral flow assay (LFA), enabling efficient ISF collection and immediate visual confirmation of test results. The HP-SML device demonstrated a 5-fold increase in ISF extraction speed compared to conventional SML devices, achieving 1.5 μL min<small>⁻¹</small> extraction rates with minimal tissue disruption and rapid skin recovery within 15 minutes. The device successfully detected C-reactive protein (CRP) at a clinically relevant limit of 10<small>⁴</small> ng mL<small>⁻¹</small>, confirming its applicability for Hodgkin lymphoma (HL) prognosis monitoring. It also exhibited high specificity for CRP, with no cross-reactivity observed against other inflammatory biomarkers such as IL-6, TNF-α, MMP-9, and MMP-2. Furthermore, ISF-derived CRP levels strongly correlated with serum CRP concentrations, validating ISF as a viable alternative for HL management. Given its high efficiency, ease of use, and on-site detection capabilities, the HP-SML device presents a significant advancement in point-of-care diagnostics and continuous disease monitoring.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 11","pages":" 2757-2768"},"PeriodicalIF":6.1,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143914794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a novel point-of-care device to monitor arterial thrombosis","authors":"Christopher A. Bresette, Viviana Claveria and David N. Ku","doi":"10.1039/D5LC00061K","DOIUrl":"10.1039/D5LC00061K","url":null,"abstract":"<p >Arterial thrombosis is a leading cause of heart attacks and strokes, representing a significant global health challenge. Microfluidic research studies have identified high shear stress, a thrombotic surface, and the presence of von Willebrand factor (vWF) and platelets as key conditions necessary for formation of arterial thrombi, termed shear-induced platelet aggregation (SIPA). However, current point-of-care (POC) assays of platelet function fail to incorporate these conditions, often relying on artificial agonists alone for stimulation. This study introduces a novel POC device designed to replicate high shear arterial thrombosis to create large platelet-rich clots reliably with small blood samples. The device was tested with blood from 10 healthy donors, with and without treatment with antiplatelet agents ASA, 2MeSAMPS and eptifibatide. The POC endpoint was compared with the PFA-100 to demonstrate novelty. A novel POC was successfully developed that can run with 5 mL of blood, had an intra-patient variability &lt;15% and could distinguish differences in the healthy subjects tested. The POC was sensitive to antiplatelet agents acetyl-salicylic acid, 2-MeSAMPS and eptifibatide, showing an increase in end volume, a proxy for occlusion time, after treatment (<em>p</em> &lt; 0.001). The novel POC device provides a unique endpoint that is uncorrelated with PFA-100 results. The ability of the novel POC to differentiate individual's thrombotic potential underscores its utility for clinical applications such as diagnosing platelet dysfunction, quantifying thrombotic risk, and optimizing antiplatelet therapies. This novel approach bridges the gap between research assays and practical clinical tools, offering a significant advancement in personalized cardiovascular care.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 11","pages":" 2684-2695"},"PeriodicalIF":6.1,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d5lc00061k?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143893314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation of cell-sized liposomes using laser-induced microjets†","authors":"Jiajue Ji, Shuma Kawai, Rina Takagi, Keiichiro Koiwai, Ryuji Kawano and Yoshiyuki Tagawa","doi":"10.1039/D5LC00149H","DOIUrl":"10.1039/D5LC00149H","url":null,"abstract":"<p >Cell-sized liposomes are microcapsules composed of a lipid bilayer, with potential applications in membrane science and synthetic biology. In this study, we present a novel method that employs high-speed laser-induced microjets to penetrate a lipid-carrying oil phase, thereby generating cell-sized liposomes. By simply triggering the microjets, we can reliably and repeatedly generate cell-sized liposomes, thereby enabling on-demand liposome production. We employed a high-speed camera to capture and analyze the penetration behavior of microjets. Additionally, we confirmed the unilamellarity of the liposomes using melittin, confirming their suitability for various biochemical applications. Furthermore, we conducted a numerical analysis to investigate potential factors influencing liposome formation in detail. These findings hold promise for advancing on-demand liposome production and contributing to the development of biochemical research.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 11","pages":" 2644-2653"},"PeriodicalIF":6.1,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143893518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elevating single-particle encapsulation in droplet microfluidics by utilizing surface acoustic wave and flow control†","authors":"Chunhua He, Huasheng Zhuo, Canfeng Yang, Jianxin Wang, Xian Jiang, Fan Li, Chengxu Lin, Hai Yang, Tuying Yong, Xiangliang Yang, Zhiyong Liu, Yan Ma, Lei Nie, Guanglan Liao and Tielin Shi","doi":"10.1039/D4LC00787E","DOIUrl":"10.1039/D4LC00787E","url":null,"abstract":"<p >Target particle encapsulation is crucial in droplet microfluidics for high-throughput applications like single-cell sequencing and drug screening. However, it faces limitations, with encapsulation rates of only 10% to 30% due to suspension density and the inherent functionality of the chip being restricted by the Poisson distribution. This leads to reagent waste and reduced effectiveness in applications requiring ultra-high multiplexing or extensive particle analysis, due to the massive empty droplets. Here we propose a droplet microfluidic system integrating surface acoustic wave (SAW) and sheath flow control. Suspensions of varying concentrations within the channel are initially pre-focused by sheath fluid, and then acoustically focused into a linear arrangement by SAW. Spacing between particles can be regulated by modulating the sheath fluid, ensuring sequential encapsulation of cells or beads in individual droplets. Thermal shock generated by the SAW, particle and droplet frequency, and particle encapsulation ratio are all elaborately evaluated. The results demonstrate that our system reaches an exciting single-bead packing efficiency of up to 78%, and achieves a packing rate of more than 60% for both high and low concentrations of solutions for polystyrene microspheres, magnetic beads and H22 cells, 6 times higher than the theoretical upper limit of the conventional method and 1.8 times higher than the Poisson distribution. More importantly, our system is designed to be free of structural and parametric constraints, which is quite important in future practical application. Thus, our on-chip particle focusing control method and droplet microfluidic system provide great potential in biological applications needing a high single-particle encapsulation ratio in limited partitions, such as ultra-high multiplex digital biomolecular detection, single-cell analysis, drug screening, and single exosome detection.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 11","pages":" 2669-2683"},"PeriodicalIF":6.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143893316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An acoustic levitation platform for high-content histological analysis of 3D tissue culture†","authors":"Emilie Vuille-dit-Bille, Céline Loussert Fonta, Sarah Heub, Stéphanie Boder-Pasche, Mahmut Selman Sakar and Gilles Weder","doi":"10.1039/D5LC00153F","DOIUrl":"10.1039/D5LC00153F","url":null,"abstract":"<p >Miniaturized three-dimensional (3D) cell culture systems, in particular organoids and spheroids, hold great potential for studying morphogenesis, disease modeling, and drug discovery. However, sub-cellular resolution 3D imaging of these biological samples remains a challenge. Histology, the gold standard for <em>ex vivo</em> microscopic interrogation of tissue anatomy, may address this challenge once the associated techniques are adapted. Due to their small size and delicate structure, organoids must be embedded in a supporting hydrogel. The histological sections have low information content because the distribution of the organoids within the gel is not controlled. To address this issue, we introduce an acoustic micromanipulation platform that concentrates and aligns organoids within a histology-compatible hydrogel block. Utilizing an array of micromachined lead zirconate titanate (PZT) transducers, the platform generates localised and precisely controlled acoustic standing waves to levitate organoids to a prescribed plane and fix their positions within a polyethylene glycol diacrylate (PEGDA)-gelatine hydrogel. Organoids from different culture conditions can be co-embedded in a traceable fashion with the use of a custom-design hydrogel grid. Our results demonstrate that more than 70% of spheroids can be positioned within a 150 μm-thick hydrogel block, substantially increasing the information content of histology sections. The platform's versatility, scalability, and ease of use will make histological assessment accessible to every life science laboratory.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 11","pages":" 2732-2743"},"PeriodicalIF":6.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d5lc00153f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143914796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A membrane-based immunosensor enabling high antifouling performance and sensitive molecular recognition.","authors":"Hiroki Yamashita,Hiroto Okuyama,Takeo Yamaguchi","doi":"10.1039/d5lc00031a","DOIUrl":"https://doi.org/10.1039/d5lc00031a","url":null,"abstract":"The fouling of non-targeted biomolecules on sensing surfaces, which can cause a reduction in sensing performance, is a severe problem in immunosensing platforms. The incorporation of hydrophilic polymers on sensing surfaces is effective against antifouling. However, such an approach can reduce the density of the capture antibody, resulting in a decrease in sensitivity and signal output. Here, both high sensitivity and antifouling properties were achieved using a porous-membrane-based immunosensor. This sensor can drastically mitigate the signal reduction due to the introduction of an antifouling moiety by antibody densification in submicron-scaled pores. The ideal ratio of the receptor/antifouling moiety was estimated from numerical modeling. The high sensitivity and antifouling properties of the designed sensor were demonstrated via the detection test of interleukin-6 (IL-6). The proposed sensor exhibited excellent antifouling and high sensitivity with limits of detection of 4.8 and 1.2 pg mL-1 in artificial saliva and serum, respectively. The study findings highlight the potential of membrane-based sensors for practical diagnoses.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"42 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143889223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrative round window membrane/cochlear microphysiological system with sensing components for the study of real-time drug response†","authors":"Jing Bai, Olurotimi Bolonduro, Pavlo Gordiichuk, R. Madison Green, Henry Hung-Li Chung, Ken Mahmud and Dmitry Shvartsman","doi":"10.1039/D4LC01025F","DOIUrl":"10.1039/D4LC01025F","url":null,"abstract":"<p >Most hearing loss often results from permanent damage to cochlear hair cells, and effective treatments remain limited. A reliable, scalable, and physiologically relevant ear model can accelerate the development of hearing-loss protection therapeutics for injury prevention and hearing restoration. The challenge remains on screening delivery systems for regenerative compounds, and no <em>in vitro</em> screening systems exist that capture the complexity of inner ear properties. Here, we present a high-throughput, microphysiological system (MPS) featuring a round window membrane (RWM) model co-cultured with murine auditory hair cells. It is integrated with a transepithelial electrical resistance (TEER) sensor module to monitor epithelial barrier function development in continuous measurements, without sacrificing a sample and thus allowing “real-time” monitoring of the RWM construct progress. The MPS integrates a syringe pump, tissue compartment, multi-channel fluid distributor, and sensors into a microfluidic continuous-flow system, allowing for on-demand sample collections of analytes triggered by the cellular response to the introduced compounds. Drug screening was conducted with protective antibiotic, antioxidant, and anti-inflammatory compounds. RWM cell and hair cell viability, TD<small>₅₀</small> values, and membrane integrity were measured. In addition, we also designed a graphene field-effect transistor (GFET)-based cytokine sensor to study proinflammatory cytokine release from cells during damaging exposure. The system was employed to assess drug diffusion efficiency, cell viability, and the drug's TD<small>₅₀</small> and compared to published data from animal studies. Cell membrane integrity was also analyzed, and proinflammatory cytokine release was measured using a GFET sensor. We evaluated and monitored the real-time structural integrity of the RWM epithelial barrier using the integrated TEER sensor in the MPS. The sensor's ability to measure TEER and cytokine levels was validated by comparing its readings to those obtained from commercial TEER signal processing equipment and standard cytokine concentration measurements. This ear-on-a-chip design enables high-throughput screening of investigational new drugs, reducing the need for animal models in complex studies of inner ear damage and regeneration. It allows for the real-time study of drug responses. It facilitates the development and identifying novel agents that protect against hearing loss and the design of delivery methods for hearing regeneration compounds.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 11","pages":" 2744-2756"},"PeriodicalIF":6.1,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143914795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}