Lab on a Chip最新文献_第5页

Signal Amplification Using Ab-AuNPs Integrated with LDI-MS Analysis for Diabetes Screening in Urine and Saliva 利用Ab-AuNPs与LDI-MS分析相结合的信号放大技术筛查尿和唾液中的糖尿病

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-09-25 DOI: 10.1039/d5lc00700c

Li-Sin Tu, Tai-Wei Liu, He-Hsuan Hsiao

{"title":"Signal Amplification Using Ab-AuNPs Integrated with LDI-MS Analysis for Diabetes Screening in Urine and Saliva","authors":"Li-Sin Tu, Tai-Wei Liu, He-Hsuan Hsiao","doi":"10.1039/d5lc00700c","DOIUrl":"https://doi.org/10.1039/d5lc00700c","url":null,"abstract":"The global prevalence of diabetes is rising at an alarming rate, making it the third leading cause of death worldwide. This study presented a user-friendly, straightforward, and non-invasive method for screening diabetes. Various antibody-conjugated boronic acid-modified gold nanoparticles (Ab-AuNPs) were prepared, including anti-HbA1c, anti-HBA1, anti-HSA, anti-gHSA, and anti-insulin, to enable the specific recognition of their corresponding antigens in single droplet samples of urine and saliva on nitrocellulose membranes, with subsequent analysis performed using laser desorption/ionization mass spectrometry (LDI-MS). Ab-AuNPs absorbed ultraviolet laser light, leading to the direct desorption and ionization of Au+ ions. This process eliminated the need for an additional organic matrix in matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS), effectively reduced interference from matrix-related ions, and significantly amplified the detection signal of Au+ ions at trace levels for targeted antigens in urine and saliva. The developed method revealed elevated levels of glycated proteins, including glycated hemoglobin (HbA1c) and glycated human serum albumin (gHSA), as well as human serum albumin (HSA), in diabetes patients compared to healthy individuals. In contrast, insulin levels were notably lower in diabetes patients. By analyzing these biomarker changes, we successfully identified the presence of diabetes. The reported method for screening diabetes in biological fluids provides a practical approach and holds significant promise for analyzing other diseases as corresponding biomarkers are discovered and their antibodies are developed and acquired in the future.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"26 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145133534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From startup to shutdown: the dramatic rise and fall of the first at-home combo test for flu and COVID-19 从启动到关闭：第一个家庭流感和COVID-19组合测试的急剧上升和下降

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-09-25 DOI: 10.1039/D5LC00305A

Morgan N. Greenleaf, Gregory L. Damhorst, Eric M. Vogel, Greg S. Martin and Wilbur A. Lam

{"title":"From startup to shutdown: the dramatic rise and fall of the first at-home combo test for flu and COVID-19","authors":"Morgan N. Greenleaf, Gregory L. Damhorst, Eric M. Vogel, Greg S. Martin and Wilbur A. Lam","doi":"10.1039/D5LC00305A","DOIUrl":"10.1039/D5LC00305A","url":null,"abstract":"This article explores the development and commercialization of Lucira Health's innovative at-home molecular diagnostic test, which detects influenza A or B and SARS-CoV-2. Launched amidst the urgent demand for accessible testing solutions, Lucira's product represented a significant breakthrough, becoming the first over-the-counter combination test authorized by the US Food and Drug Administration (FDA). The narrative tracks Lucira's journey from its origins in microfluidics at the University of California-Berkeley, through development challenges, business success and failure. It also contrasts the distinct motivations and technical challenges of pre-pandemic versus pandemic era diagnostics, emphasizing test-to-treat strategies versus rapid results for containment. Despite early successes, Lucira faced insurmountable regulatory and financial hurdles, culminating in bankruptcy just days before FDA authorization. The case offers critical insights into diagnostics product development, regulatory navigation, product diversification, and strategic risk management in push towards home and point of care diagnostics.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 20","pages":" 5115-5121"},"PeriodicalIF":5.4,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d5lc00305a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145133536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

sNails: sweat-sensing nails for unobtrusive, wearable microfluidic sweat monitoring from the dorsal distal phalanges 蜗牛：汗液感应钉，用于不显眼的，可穿戴的微流体汗液监测从远端指骨背。

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-09-24 DOI: 10.1039/D5LC00586H

Noelle Davis, Pooja Mehta, Amanda Kang, Liam Gillan, Jussi Hiltunen and Ali Javey

引用次数: 0

Deterministic cell pairing with simultaneous microfluidic merging and sorting of droplets 微流体同时合并和分选的确定性细胞配对

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-09-23 DOI: 10.1039/D5LC00627A

Kevin M. Joslin, Sophia Dateshidze, Seung Won Shin, Adam R. Abate and Iain C. Clark

{"title":"Deterministic cell pairing with simultaneous microfluidic merging and sorting of droplets","authors":"Kevin M. Joslin, Sophia Dateshidze, Seung Won Shin, Adam R. Abate and Iain C. Clark","doi":"10.1039/D5LC00627A","DOIUrl":"10.1039/D5LC00627A","url":null,"abstract":"Cell–cell interactions drive immune activation, tissue repair, and stem cell fate, yet there are few methods that can create large numbers of pre-defined cell pairs to study cell crosstalk. Droplet microfluidics allows high-throughput compartmentalization of multiple cells, but random loading results in <1% of droplets containing the desired combinations. Here, we present <img>air <img>solation by <img>oalescence and <img>orting (PICS), a microfluidic platform that can generate specific cell pairs through droplet merging and sorting (‘merge-sorting’). PICS detects target combinations using fluorescence and triggers simultaneous electrocoalescence and dielectrophoretic sorting. Using fluorescent dye-loaded droplets, we achieved 98.6% purity of merged and sorted droplets. In experiments using cells stained with three distinct dyes, >90% of desired cell pairs were recovered – compared to fewer than 1% when using random Poisson loading. To demonstrate the utility of PICS for extended co-culture studies, we merged cells in an alginate solution with calcium chloride droplets, producing monodisperse alginate hydrogels in which 93.3% of the beads contained target cell pairs that maintained viability over 18 hours. Compared to selective merger, this approach physically isolates desired droplets, eliminating unmerged contaminants and enabling cleaner downstream workflows. PICS allows off-chip pre-incubation of droplets before pairing, the merger of reagents for multi-step assays, and the rapid isolation of desired droplet pairs – capabilities not jointly accessible with existing approaches. In summary, PICS is a flexible platform to enrich specific combinations of droplets, cells, or particles for high-throughput studies of cell crosstalk.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 21","pages":" 5497-5505"},"PeriodicalIF":5.4,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145127930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Label-Free Imaging Flow Cytometry with Rare Cell Classification using Motion-Sensitive-Triggered Interferometry 使用运动敏感触发干涉法进行罕见细胞分类的无标记成像流式细胞术

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-09-23 DOI: 10.1039/d5lc00634a

Eden Dotan, Dana Yagoda-Aharoni, Eli Shapira, Natan T. Shaked

{"title":"Label-Free Imaging Flow Cytometry with Rare Cell Classification using Motion-Sensitive-Triggered Interferometry","authors":"Eden Dotan, Dana Yagoda-Aharoni, Eli Shapira, Natan T. Shaked","doi":"10.1039/d5lc00634a","DOIUrl":"https://doi.org/10.1039/d5lc00634a","url":null,"abstract":"We present a label-free imaging flow cytometry system that integrates a microfluidic chip imaged by a motion-sensitive (event-based) camera and an interferometric-phase-microscopy module using a simple frame-based camera. The event camera captures activity from the flowing cells corresponding to thousands of frames per second and triggers the significantly slower interferometric camera when a rare cell, requiring more sensitive analysis, is detected via a single raw-interferogram analysis, significantly reduicng data volume. The raw interferometric data serves as an input to a deep neural network for rare-cell classification. We demonstrate using this system to detect and grade rare cancer cells in blood, where the event camera is used to rapidly classify between the common white blood cells and the rare cancer cells, and the interferometric camera is used to grade the cancer cell type (primary/metastatic), as a human model for detecting and grading circulating tumor cells in liquid biopsies. This hybrid approach enables efficient data acquisition, rapid processing, and high sensitivity, significantly reducing computational load, and is expected to find various applications in detecting and processing rare cells in imaging flow cytometry.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"51 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145116867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ECM-integrated hanging drop platform for spatially controlled assessment of immune cell regulated tumour invasion 免疫细胞调节肿瘤侵袭的空间控制评估- ecm集成悬滴平台

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-09-22 DOI: 10.1039/D5LC00359H

Chanyang Lee, Seokgyu Han, Seulgi Lee, Jaehyun Lee, Sein Kim, Seunggyu Ko, Howon Lee and Sungsu Park

{"title":"ECM-integrated hanging drop platform for spatially controlled assessment of immune cell regulated tumour invasion","authors":"Chanyang Lee, Seokgyu Han, Seulgi Lee, Jaehyun Lee, Sein Kim, Seunggyu Ko, Howon Lee and Sungsu Park","doi":"10.1039/D5LC00359H","DOIUrl":"10.1039/D5LC00359H","url":null,"abstract":"The tumour immune microenvironment (TIME) plays a crucial role in tumour progression and metastasis. Although spheroids effectively model tumour invasion by mimicking in vivo 3D structures, their formation and subsequent mixing with the matrix make it difficult to control their position in the 3D matrix, leading to deep embedding and hindering the assessment of immune cell-mediated regulation of invasion. This paper introduces an extracellular matrix (ECM)-integrated hanging drop platform that enables simultaneous spheroid formation and matrix incorporation, allowing precise spatial control and direct assessment of immune cell-mediated regulation of invasion. In the presence of microglia (MG), cancer cells rapidly migrate out of the spheroids through the ECM, demonstrating cancer invasion. The cytotoxic effect of natural killer (NK) cells on glioblastoma multiforme (GBM) spheroids is decreased owing to the inhibition of NK cell infiltration in the presence of MG, highlighting the immunosuppressive nature of the TIME. However, inhibiting signal transducer and activator of transcription 3 (STAT3) activation with drugs halts MG-induced immunosuppression and enhances NK cell infiltration. This model enables efficient high-throughput screening and is the first to allow for precise quantification of the effects of the STAT3 inhibitor on tumour invasion, immune cell movement, and behaviour within a physiologically relevant GBM TIME model.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 21","pages":" 5617-5627"},"PeriodicalIF":5.4,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145116868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Sperm Beating Characteristics Using Microfluidic Trapping and Machine-Learning-Based Flagellum Tracking 基于微流体捕获和机器学习的鞭毛跟踪的精子跳动特性分析

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-09-20 DOI: 10.1039/d5lc00389j

Aisha Hamidu, Omar Abdelgawad, Ahmed Azmeer, Mohamed Abdelgawad

{"title":"Analysis of Sperm Beating Characteristics Using Microfluidic Trapping and Machine-Learning-Based Flagellum Tracking","authors":"Aisha Hamidu, Omar Abdelgawad, Ahmed Azmeer, Mohamed Abdelgawad","doi":"10.1039/d5lc00389j","DOIUrl":"https://doi.org/10.1039/d5lc00389j","url":null,"abstract":"Male infertility affects a significant portion of couples worldwide, with standard semen analysis often failing to identify functional deficiencies in sperm performance. This study presents a microfluidic platform for characterizing sperm flagellar beating patterns with unprecedented detail, providing insights into sperm functional parameters potentially linked to unexplained infertility. We combined microcontact printing of fibronectin adhesion spots with machine-learning-based flagellum tracking to immobilize sperm heads while allowing free flagellar movement, enabling precise analysis of beating characteristics. Our tracking algorithm utilizes YOLOv8 (You Only Look Once) machine learning-based computer vision model and which we trained using 750 manually annotated images of sperm cells. We used keypoint detection along the sperm flagellum to calculate critical beating parameters including the beating amplitude, frequency, and asymmetry patterns. To validate the platform, we investigated the effects of established capacitation and hyperactivation agents on sperm motility. Caffeine treatment (10-40 mM) increased flagellar beat amplitude by up to 65% while decreasing frequency by approximately 50%, with pronounced asymmetrical beating consistent with hyperactivation. Heparin exposure (10-100 μg/ml) similarly enhanced beating amplitude by approximately 25% without significantly altering frequency. We also analysed the beating pattern of sperm cells immobilized inside a microchannel under different flow velocities. Results revealed a decrease in the beating frequency when sperm cells were subjected to flow. The platform eliminates the need for sophisticated sperm tracking techniques which facilitates high-throughput analysis under controlled physicochemical conditions. By enabling detailed characterization of sperm flagellar behaviour under various stimuli, our platform offers a valuable tool for investigating molecular mechanisms underlying idiopathic male infertility and evaluating potential therapeutic interventions.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"17 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145089586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: A nanobody-based microfluidic chip for fast and automated purification of protein complexes 更正：一种基于纳米体的微流控芯片，用于快速和自动纯化蛋白质复合物

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-09-19 DOI: 10.1039/D5LC90098K

Phebe De Keyser, Mitch de Waard, Ignaas S. M. Jimidar, Sandrien Verloy, Steven Janvier, Valentina Kalichuk, Thomas Zögg, Alexandre Wohlkönig, Els Pardon, Jan Steyaert and Gert Desmet

引用次数: 0

Microfluidic single-cell drug screening: toward personalized precision therapy in chronic myeloid leukemia 微流控单细胞药物筛选：迈向慢性髓性白血病个体化精准治疗

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-09-18 DOI: 10.1039/D5LC00779H

Hwisu Jeon, Yukyung Park, Soo-Hyun Kim, Chang-Yeol Jung, Hongtae Kim, Eujin Um, Dong-Wook Kim and Taesung Kim

{"title":"Microfluidic single-cell drug screening: toward personalized precision therapy in chronic myeloid leukemia","authors":"Hwisu Jeon, Yukyung Park, Soo-Hyun Kim, Chang-Yeol Jung, Hongtae Kim, Eujin Um, Dong-Wook Kim and Taesung Kim","doi":"10.1039/D5LC00779H","DOIUrl":"10.1039/D5LC00779H","url":null,"abstract":"Chronic myeloid leukemia (CML) treatment employs several FDA-approved BCR::ABL1 tyrosine kinase inhibitors (TKIs) with distinct efficacy and side effects influenced by patient-specific factors. This study introduces a microfluidic cell culture array for the comparative analysis of six BCR::ABL1 TKIs, namely imatinib, nilotinib, bosutinib, ponatinib, dasatinib, and asciminib, using CML-related cell lines. The device provides a continuous, chemostat-like microfluidic environment that enables quantitative drug sensitivity scoring. The microchambers for cell culture notably offer advantages for single-cell imaging of suspension leukemia cells, which tend to aggregate in conventional culture platforms. This system supports the detailed characterization of cell viability across various TKI types and concentrations, yielding comprehensive mathematical metrics to assess relative drug efficacy. In this study, we compared drug responses in K562 and Ba/F3 BCR::ABL1 cell lines, including the T315I mutant variant, and specifically demonstrated that Ba/F3 cells harboring the T315I mutation exhibit resistance to the first- and second-generation TKIs, responding only to ponatinib and asciminib. We further validated the device with a CML patient-derived bone marrow sample, requiring only minimal adjustments to the experimental conditions. The proposed microfluidic single-cell-based screening array could refine treatment regimens and advance personalized medicine in CML.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 21","pages":" 5638-5652"},"PeriodicalIF":5.4,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145077679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel holder and microfluidic system for spatially controlled hypoxia induction, mechanical stimulation and cardiac regeneration research 一种用于空间控制缺氧诱导、机械刺激和心脏再生研究的新型支架和微流体系统

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-09-18 DOI: 10.1039/D5LC00460H

Kołodziejek Dominik, Szlachetka Aleksandra, Iwoń-Szczawińska Zuzanna, Drozd Marcin, Wojasiński Michał and Jastrzębska Elżbieta

{"title":"A novel holder and microfluidic system for spatially controlled hypoxia induction, mechanical stimulation and cardiac regeneration research","authors":"Kołodziejek Dominik, Szlachetka Aleksandra, Iwoń-Szczawińska Zuzanna, Drozd Marcin, Wojasiński Michał and Jastrzębska Elżbieta","doi":"10.1039/D5LC00460H","DOIUrl":"10.1039/D5LC00460H","url":null,"abstract":"Over the years, cardiovascular diseases have remained a leading cause of mortality worldwide, necessitating advanced experimental models to mimic the natural environment of the heart as closely as possible. New microfluidic heart models would enable precise modelling of the healthy heart as well as pathological mechanisms occurring during cardiac ischemia and testing new therapies would become more accessible than it is currently. In this study, we developed a two layer holder for creating hypoxic conditions in a dedicated microfluidic system for modelling cardiac infarction. By integrating the holder with a microsystem utilizing nanofibrous mats with iron(II, III) oxide (Fe3O4) magnetic nanoparticles we were able to create a tool for using (potentially patient specific) induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) in cardiac research. In the microsystem we induced hypoxia and conducted preliminary observations of cardiac regeneration using induced pluripotent stem cells (iPSCs). We found that iPSCs express cTnT when co-cultured with hypoxia-damaged iPSC-CMs indicating that the holder with an integrated microsystem can be used for hypoxia and cardiac regeneration studies.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 21","pages":" 5524-5537"},"PeriodicalIF":5.4,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d5lc00460h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145077683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0