Lab on a Chip最新文献_第5页

Enhancing LIBS detection of oil-borne metallic microparticles via acoustic manipulation chip-based enrichment. 基于声学操作芯片的富集增强油基金属微粒的LIBS检测。

IF 6.3 2区工程技术

Lab on a Chip Pub Date : 2026-09-29 DOI: 10.1039/d6lc00501b

Xiaolong Lu, Zhexiao Zhu, Zihao Tang, Yijia Liu, Ying Wei, Gai Zhao, Wenjuan Liu

{"title":"Enhancing LIBS detection of oil-borne metallic microparticles via acoustic manipulation chip-based enrichment.","authors":"Xiaolong Lu, Zhexiao Zhu, Zihao Tang, Yijia Liu, Ying Wei, Gai Zhao, Wenjuan Liu","doi":"10.1039/d6lc00501b","DOIUrl":"10.1039/d6lc00501b","url":null,"abstract":"Metallic wear debris in lubricating oils can reflect abnormal wear conditions in rotating machinery, and the rapid analysis of chemical elements in wear particles can timely predict the exact fault positions, thereby enabling ordinary operation and maintenance support in diverse industrial applications. Emerging as a quick and convenient analytical tool, laser-induced breakdown spectroscopy (LIBS) is promising for directly detecting oil-borne metallic microparticles. However, its efficiency has been greatly hindered by the low concentration of micrometric particles sparsely distributed in the oil matrix. Here, we present an acoustic manipulation strategy for enhancing the LIBS detection of oil-borne metallic microparticles. A sandwich-type configuration was selected to build the acoustic manipulation chip with dual parallelized serpentine microchannels, and the acoustic radiation force dominates the quick enrichment of randomly distributed metallic particles. With increased particle concentration, the LIBS spectral intensity was significantly enhanced for 3 typical elements (Fe, Cu, and Ni). The experimental results illustrated that the large background noise interference from the oil matrix was effectively suppressed, and the metallic elements after enrichment could be accurately recognized. The proposed acoustic enrichment method lays a solid foundation for enhancing the LIBS detection of oil-borne metallic particles, offering a new alternative for quick fault diagnosis and in-time health management of mechanical rotating systems in practical applications.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":"5163-5175"},"PeriodicalIF":6.3,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148838569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laminin - myCAF interplay promotes α6-integrin-mediated breast cancer cell invasion. 层粘连蛋白- myCAF相互作用促进α6-整合素介导的乳腺癌细胞侵袭。

IF 6.3 2区工程技术

Lab on a Chip Pub Date : 2026-09-29 DOI: 10.1039/d5lc00711a

María Virumbrales-Muñoz, Shelby A Fertal, Karina M Lugo-Cintrón, Jack R Loken, Yalmarie Numan-Vazquez, Brian M Burkel, Jing Zhang, Christian Franck, José M Ayuso, David J Beebe, Suzanne M Ponik

{"title":"Laminin - myCAF interplay promotes α6-integrin-mediated breast cancer cell invasion.","authors":"María Virumbrales-Muñoz, Shelby A Fertal, Karina M Lugo-Cintrón, Jack R Loken, Yalmarie Numan-Vazquez, Brian M Burkel, Jing Zhang, Christian Franck, José M Ayuso, David J Beebe, Suzanne M Ponik","doi":"10.1039/d5lc00711a","DOIUrl":"10.1039/d5lc00711a","url":null,"abstract":"It is well established that the tumor microenvironment (TME) plays a dynamic role in breast cancer progression. Stromal components of the TME, including fibroblasts and the extracellular matrix (ECM), provide biophysical and biochemical cues to drive tumor cell proliferation and invasion. However, how the interplay between activated fibroblast (CAF) subtypes and ECM composition impinges on breast cancer cell behavior is still not well understood. To address this gap, we leveraged microfluidic technologies to engineer a model of the breast TME that incorporates tumor cells, CAFs, and ECM components. Our model consists of a tubular duct filled with MDA-MB-231 or MCF-7 cells surrounded by a 3D collagen type I matrix with or without two human fibroblasts/CAFs. To further investigate how ECM composition affects the stromal compartment, we altered the matrix to include laminin-111 (LN) and thrombospondin-1 (TSP-1), which we previously found to be dysregulated in invasive ductal carcinoma compared to normal breast tissue. By incorporating LN or TSP-1 into the collagen matrix, we identified significant differences in collagen fiber metrics (fiber length, density, and width), matrix stiffness, and alterations in matrix remodeling by CAFs. Specifically, remodeling was more pronounced in LN- vs. TSP-1-enriched microenvironments populated by myofibroblast-like (myCAF-like) cells compared to inflammatory CAFs (iCAF-like). Notably, MDA-MB-231 cell proliferation and invasion were significantly enhanced under these same conditions, and invasion was dependent on the LN-binding receptor, α6-integrin.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":"5216-5231"},"PeriodicalIF":6.3,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13523129/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148838590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A compact centrifugal multi-volume digital PCR disk (MV-dPCR disk) with integrated pneumatic stabilization. 一个紧凑的离心多体积数字PCR磁盘（MV-dPCR磁盘）集成气动稳定。

IF 6.3 2区工程技术

Lab on a Chip Pub Date : 2026-09-29 DOI: 10.1039/d6lc00381h

Yu-Kai Lai, Marvin Heyer, Silvia Calabrese, Tobias Hutzenlaub, Nils Paust, Jacob F Hess

{"title":"A compact centrifugal multi-volume digital PCR disk (MV-dPCR disk) with integrated pneumatic stabilization.","authors":"Yu-Kai Lai, Marvin Heyer, Silvia Calabrese, Tobias Hutzenlaub, Nils Paust, Jacob F Hess","doi":"10.1039/d6lc00381h","DOIUrl":"10.1039/d6lc00381h","url":null,"abstract":"Digital polymerase chain reaction (dPCR) enables absolute nucleic acid quantification with high sensitivity and has strong potential for decentralized and integrated analytical applications. However, most current dPCR platforms rely on bulky pressure-driven instrumentation and large-footprint cartridges, limiting their suitability for compact and fully integrated microfluidic systems that incorporate upstream sample processing. Here, we introduce a centrifugal multi-volume digital PCR (MV-dPCR) disk designed to perform dPCR assays. The disk incorporates two primary innovations: (i) a radially arranged, multi-scale partitioning array with four distinct microcavity volumes (60 nl, 12 nl, 2.4 nl, and 0.48 nl), achieving compact layout while preserving a broad dynamic range; and (ii) an integrated vapor-based pneumatic pressurization module (∼70 kPa) that maintains stable partition integrity during thermal cycling without requiring external pressure hardware. These features are implemented in a monolithic cyclic olefin copolymer (COC) cartridge fabricated via thermoforming and sealed through thermo-diffusive bonding, occupying only one-third of a standard 140 mm disk. This compact design preserves plentiful space for future integration of upstream sample preparation modules. The MV-dPCR disk consistently achieves uniform partition filling with volume coefficients of variation (CVs) below 12% and low dead volume (<9%), and covers a 4.6 log10 dynamic range using only 720 partitions. Validation experiments using synthetic DNA over four ten-fold dilution series demonstrate excellent linearity (R2 > 0.99) and quantification accuracy comparable to commercial dPCR systems. Collectively, these advancements render the MV-dPCR disk a versatile, affordable, and easily scalable platform with potential for integration into more complex automated microfluidic workflows.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":"5327-5338"},"PeriodicalIF":6.3,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148823837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemically bonded microfluidic device with integrated laser-induced graphene electrodes. 集成激光诱导石墨烯电极的化学键合微流控装置。

IF 6.3 2区工程技术

Lab on a Chip Pub Date : 2026-09-29 DOI: 10.1039/d6lc00532b

Lema Amiry, Rose K Phull, Darius G Rackus

{"title":"Chemically bonded microfluidic device with integrated laser-induced graphene electrodes.","authors":"Lema Amiry, Rose K Phull, Darius G Rackus","doi":"10.1039/d6lc00532b","DOIUrl":"10.1039/d6lc00532b","url":null,"abstract":"The integration of electrochemical sensing with microfluidic platforms is essential for advancing lab-on-a-chip technologies; however, many existing approaches rely on electrodes fabricated using costly and complex techniques such as thin-film deposition and photolithography. Laser-induced graphene (LIG) offers a rapid and low-cost alternative for electrode fabrication, yet it is most commonly produced on polyimide substrates, which are not readily compatible with permanent bonding to microfluidic channels and typically require adhesives or mechanical clamping. Here, we present a novel approach to integrating LIG electrodes with microchannels fabricated in polydimethyl siloxane (PDMS), a common material for microfluidics. Electrodes were formed on SU-8 photoresist-coated glass slides using a CO2 laser and then covalently bonded to PDMS via 3-aminopropyl triethoxysilane (APTES). Structural and spectroscopic characterization of the LIG on SU-8 demonstrate successful conversion into conductive turbostratic graphitic carbon, while mechanical studies demonstrate strong bond integrity between the electrode substrate and PDMS over 16 hours of continuous operation. Electrochemical validation of the electrodes both off- and on-chip demonstrate performance suitable for quantitative electroanalysis. Proof-of-principle application of the integrated device to the detection of acetaminophen (paracetamol) by differential pulse voltammetry under flow conditions reveals a clinically relevant limit of detection (0.08 mM) with high intra-device precision. The resulting platform enables seamless integration with microfluidic channels and exhibits robust electrochemical performance ideal for rapid prototyping.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":"5152-5162"},"PeriodicalIF":6.3,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148785713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous detection of influenza and SARS-CoV-2 on an AI–nanopore multiplex platform 人工智能纳米孔复合平台同时检测流感和SARS-CoV-2

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2026-09-28 DOI: 10.1039/d6lc00155f

Keiichiroh Akabane,Kaoru Murakami,Yaze Wang,Takayuki Takaai,Takashi Washio,Keita Tagami,Kazuki Sato,Yuichi Kojima,Kenta Sasaki,Hironi Makita,Takeshi Hattori,Satoshi Konno,Masaaki Murakami,Masateru Taniguchi

{"title":"Simultaneous detection of influenza and SARS-CoV-2 on an AI–nanopore multiplex platform","authors":"Keiichiroh Akabane,Kaoru Murakami,Yaze Wang,Takayuki Takaai,Takashi Washio,Keita Tagami,Kazuki Sato,Yuichi Kojima,Kenta Sasaki,Hironi Makita,Takeshi Hattori,Satoshi Konno,Masaaki Murakami,Masateru Taniguchi","doi":"10.1039/d6lc00155f","DOIUrl":"https://doi.org/10.1039/d6lc00155f","url":null,"abstract":"Seasonal influenza and SARS-CoV-2 co-circulate and present overlapping symptoms, creating demand for rapid multiplex diagnostics beyond the sensitivity limits of antigen tests and the infrastructure requirements of nucleic acid amplification tests. Here we report NanoMux, an amplification-free diagnostic platform that couples a solid-state nanopore with attention-based multiple-instance learning (Attention-MIL) to classify clinical saliva samples from their nanopore event streams. Each measurement is treated as a “bag” of unlabeled translocation events dominated by endogenous nanoparticles, while only a sparse subset contains virus like signatures. Attention-MIL learns to weight informative events during bag-level prediction, enabling robust classification in highly contaminated clinical matrices where conventional machine learning baselines degrade. In a three-class setting (SARS-CoV-2, influenza, and healthy controls), Attention-MIL achieved a macro F1 of 0.781, substantially outperforming a gradient-boosting baseline (0.327). Under diluted conditions, the LightGBM model exhibited a more gradual decline in accuracy than the Attention-MIL model, demonstrating greater robustness for low-concentration specimens. NanoMux establishes a physics-based, reagent-minimal route to rapid multiplex respiratory virus screening and provides a model framework for extracting sparse pathogen signals from noisy nanopore recordings.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"219 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149067380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vascularized glioblastoma-on-a-chip reveals microglia-mediated regulation of tumor invasion and stemness. 血管化胶质母细胞瘤芯片揭示了小胶质细胞介导的肿瘤侵袭和干细胞的调控。

IF 6.3 2区工程技术

Lab on a Chip Pub Date : 2026-09-25 DOI: 10.1039/d6lc00475j

Twinkle Jina Minette Manoharan, Kalpana Ravi, Shwetal Mehta, Mehdi Nikkhah

{"title":"Vascularized glioblastoma-on-a-chip reveals microglia-mediated regulation of tumor invasion and stemness.","authors":"Twinkle Jina Minette Manoharan, Kalpana Ravi, Shwetal Mehta, Mehdi Nikkhah","doi":"10.1039/d6lc00475j","DOIUrl":"https://doi.org/10.1039/d6lc00475j","url":null,"abstract":"Glioblastoma multiforme (GBM) is the most aggressive primary brain tumor in adults, characterized by rapid progression and poor prognosis. Central to GBM pathobiology are glioma stem cells (GSCs), which preferentially localize within the perivascular niche (PVN) and sustain tumor growth, invasion, and therapeutic resistance through dynamic interactions with the surrounding PVN microenvironment. Notably, microglia (MG), the brain-resident immune cells, represent a substantial fraction of the GBM PVN and play a pivotal role in establishing an immunosuppressive tumor microenvironment (TME). However, the mechanisms by which MG interacts with the brain PVN and how this, in turn, regulates GSC behavior remain poorly understood. To systematically investigate these complex cellular interactions, herein we engineered a compartmentalized human GBM PVN-on-a-Chip (GPoC) model featuring a perfusable brain microvascular network surrounding a tumor core composed of patient-derived GSCs and a stromal region containing brain-resident MG. The presented biomimetic 3D model recapitulates the structural and functional features of the native PVN, enabling well-controlled experimental interrogation and spatial visualization of tumor-immune-vascular crosstalk. Through invasion assay, real-time live imaging, immunofluorescent staining, and secretome profiling, it was evident that the GSC cells exhibited enhanced invasion in the presence of both MG and vasculature. Furthermore, pharmacological intervention studies revealed attenuated GB3 migration under tri-culture conditions, highlighting that the migratory phenotype of the tumor cells is, in part, driven by MG. Overall, the engineered GPoC platform serves as a physiologically relevant human brain tumor model for dissecting MG-mediated mechanisms and advancing therapeutic targeting of the GBM microenvironment.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149035553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heart–Lung-on-a-Chip Featuring Dynamic Flow-Resistance Capture for Drug-Associated Cardiac Dysfunction Assessment under Tumor–Immune-Conditioned Drug Exposure 在肿瘤免疫条件药物暴露下，心脏-肺芯片具有动态血流阻力捕获的药物相关心功能障碍评估

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2026-09-23 DOI: 10.1039/d6lc00764c

He-Jhen Chen,Tien-Chun Yang,Wei-Lun Sun,Chun-Yao Huang,Cheng-Hsien Liu,Kang-Yun Lee

{"title":"Heart–Lung-on-a-Chip Featuring Dynamic Flow-Resistance Capture for Drug-Associated Cardiac Dysfunction Assessment under Tumor–Immune-Conditioned Drug Exposure","authors":"He-Jhen Chen,Tien-Chun Yang,Wei-Lun Sun,Chun-Yao Huang,Cheng-Hsien Liu,Kang-Yun Lee","doi":"10.1039/d6lc00764c","DOIUrl":"https://doi.org/10.1039/d6lc00764c","url":null,"abstract":"A dual-layer heart–lung-on-a-chip integrating flow-resistance capture was developed to enable the assessment of cardiac responses to lung cancer therapeutics under tumor–immune-conditioned exposure. The compartment incorporates an A549–Jurkat tumor–immune co-culture and exposure to chemotherapy and immune checkpoint inhibitors.. The lower heart compartment incorporates a dynamic flow-resistance capture structure that selectively traps heart organoids with diameters of 150–200 μm, ensuring stable positioning and uniform perfusion. A 4 μm porous membrane separates the compartments, enabling membrane-mediated molecular exchange and providing a simplified inter-compartment exposure model. Three agents—doxorubicin hydrochloride, pembrolizumab, and atezolizumab—were evaluated in monoculture and immune co-culture settings. Cardiac contractile activity was monitored using a custom MATLAB-based image analysis pipeline to quantify the beat rate, inter-beat interval, and heart rate variability. Immune co-culture enhanced drug-induced functional impairment, with the magnitude of immune-co-culture-associated enhancement of drug-induced cardiac functional impairment following the order: doxorubicin > pembrolizumab > atezolizumab. The lung cancer–immune co-culture setting enhanced drug-induced cardiac functional impairment compared with monoculture conditions, revealing differential cardiac functional responses that were not readily apparent in monoculture. This integrated microfluidic system combines multi-organ interactions, precise organoid capture, and automated functional readouts. This offers a reproducible and physiologically relevant approach for preclinical drug safety profiling and has potential applications in precision oncology and personalized medicine.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"47 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149019657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Magnetic Artificial Cilia Platform for Dynamic Mechanical Stimulation of Cells 磁性人工纤毛平台对细胞进行动态机械刺激

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2026-09-23 DOI: 10.1039/d6lc00821f

Emmie Johanna Dymphina Schoutens,Jelle Arends,Nicholas A. Kurniawan,Jaap den Toonder

{"title":"A Magnetic Artificial Cilia Platform for Dynamic Mechanical Stimulation of Cells","authors":"Emmie Johanna Dymphina Schoutens,Jelle Arends,Nicholas A. Kurniawan,Jaap den Toonder","doi":"10.1039/d6lc00821f","DOIUrl":"https://doi.org/10.1039/d6lc00821f","url":null,"abstract":"Mechanical forces play a key role in regulating cell behaviour, with dynamically changing mechanical cues governing processes such as morphogenesis, wound healing, and cancer progression in vivo. However, there is a lack of experimental platforms that enable localized, dynamic, and tuneable mechanical stimulation under real-time imaging. Here, we present a large deflection magnetic artificial cilia (ldMAC) platform that enables dynamic, microscale mechanical stimulation of cells with high spatial and temporal control. Arrays of ldMAC are fabricated via femtosecond lasermachining and hot embossing of a magnetic polymer, yielding robust ldMAC capable of tip deflections up to 25 µm under actuation by a custom-made, compact permanent magnet-based system, representing a several-fold increase in displacement compared to most reported magnetic pillar platforms. The setup is compatible with standard cell culture conditions and live fluorescence microscopy, a feature that is typically challenging due to the bulky and complex nature of conventional actuation systems. Using Human Dermal Fibroblasts, we demonstrate that ldMAC actuation induces changes in cell morphology without affecting viability. Dynamic stimulation by cyclic ldMAC deflection leads to a shift toward more compact, rounded cell phenotypes, accompanied by reduced cell area. Timelapse imaging reveals that cells actively reorganize cytoskeletal structures and reduce the amount of cilia attachments upon stimulation, reminiscent of strain avoidance behaviour. Compared to existing approaches, the presented platform uniquely combines localized, dynamic, controllable actuation with real-time imaging in a compact, user-friendly format. Importantly, it enables active and reversible ldMAC deformation with independently tuneable frequency and amplitude. This approach offers a practical tool for studying cellular responses to dynamic mechanical environments and opens new possibilities for mechanobiology research.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"62 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149019686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Integrated Microfluidic Platform for Tunable-Stringency Competitive Affinity Selection of Folate Receptor Alpha Aptamers 叶酸受体α适体可调强度竞争亲和选择的集成微流控平台

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2026-09-22 DOI: 10.1039/d6lc00515b

Yi-Da Chung,Yi-Cheng Tsai,Keng-Fu Hsu,Gwo-Bin Lee

{"title":"An Integrated Microfluidic Platform for Tunable-Stringency Competitive Affinity Selection of Folate Receptor Alpha Aptamers","authors":"Yi-Da Chung,Yi-Cheng Tsai,Keng-Fu Hsu,Gwo-Bin Lee","doi":"10.1039/d6lc00515b","DOIUrl":"https://doi.org/10.1039/d6lc00515b","url":null,"abstract":"This study developed a new microfluidic device for competitive affinity selection of single-stranded DNA (ssDNA) aptamers targeting folate receptor alpha (FRα), an ovarian cancer (OvCa) biomarker. The device integrated micropumps, microvalves, and micromixers to automate four selection rounds in ~2.5 h, while washing stringency was tuned by a peristaltic micropump. Increasing the library concentration across rounds yielded a 2.9-fold increase in selection power and a higher binding ratio than the constant-concentration setup. The secondary structures of aptamer candidate sequences were analyzed by mfold, and a weighted structural pattern index (WSPI) combining free energy and structural elements was introduced for ranking; competitive affinity selection increased the WSPI≥12 fraction from 7.4% in conventional systematic evolution of ligands by exponential enrichment (SELEX) approach to 9.5–11.2% across affinity-selection conditions. FRα-binding aptamers obtained by this approach were characterized by surface plasmon resonance to estimate their dissociation constant (KD), and a moderate positive correlation was observed between WSPI and −log (KD). This workflow provides a new microfluidic strategy for ranking and selecting FRα-binding aptamer candidates by using PCR-free competitive affinity selection for future biomarker-probe development.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"24 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149013576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective patterning of hybrid colloids via reconfigurable acoustic-dielectric field 通过可重构声-介电场的混合胶体的选择图型

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2026-09-21 DOI: 10.1039/d6lc00575f

Leyla Akh,Yu Gao,Xiaoyun Ding

{"title":"Selective patterning of hybrid colloids via reconfigurable acoustic-dielectric field","authors":"Leyla Akh,Yu Gao,Xiaoyun Ding","doi":"10.1039/d6lc00575f","DOIUrl":"https://doi.org/10.1039/d6lc00575f","url":null,"abstract":"Acoustophoresis and dielectrophoresis are frequently used for microscale particle and cell manipulation, yet they are often used as two separate modalities. This article presents dynamic, selective patterning of micro- and nanoparticles using a reconfigurable surface acoustic wave microfluidic platform that integrates both acoustic radiation force (ARF) and dielectrophoretic (DEP) force using a single actuation/excitation modality. This platform enables frequency-based modulation of acoustic wave polarization to dynamically control the competition between the acoustohydrodynamic (AHD) regime and the electrohydrodynamic (EHD) regime without changes to device geometry or buffer conditions. This system’s exceptional capability to selectively pattern different micro- and nanomaterials is demonstrated, which cannot be achieved with existing acoutophoresis or dielectrophoresis technologies. In particular, the utility of this platform is demonstrated in the selective patterning of live and dead mammalian cells with high reversibility and reproducibility. Together, these results demonstrate the potential of the reconfigurable acoustofluidic platform where acoustic effects create a force landscape and dielectric effects define landscape occupancy, providing a flexible and reversible framework for diverse microscale self-organization applications.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"12 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149010184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0