Lab on a Chip最新文献_第5页

Microstring-engineered tension tissues: A novel platform for replicating tissue mechanics and advancing mechanobiology 微弦工程张力组织：复制组织力学和推进机械生物学的新平台

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-11-06 DOI: 10.1039/d4lc00753k

Zixing Zhou, Tingting Li, Wei Cai, Xiaobin Zhu, Zuoqi Zhang, Guoyou Huang

{"title":"Microstring-engineered tension tissues: A novel platform for replicating tissue mechanics and advancing mechanobiology","authors":"Zixing Zhou, Tingting Li, Wei Cai, Xiaobin Zhu, Zuoqi Zhang, Guoyou Huang","doi":"10.1039/d4lc00753k","DOIUrl":"https://doi.org/10.1039/d4lc00753k","url":null,"abstract":"Replicating the mechanical tension of natural tissues is essential for maintaining organ function and stability, posing a central challenge in tissue engineering and regenerative medicine. Existing methods for constructing tension tissues often encounter limitations in flexibility, scalability, or cost-effectiveness. This study introduces a novel approach to fabricate soft microstring chips using a sacrificial template method, which is easy to operate, offers controlled preparation, and is cost-effective. Through experimental testing and finite element simulations, we validated and characterized the relationship between microstring deformation, tissue width, and the reaction force exerted by the microstrings, enabling precise measurement of tissue contraction force. We successfully constructed microstring-engineered tension tissues (METTs) and demonstrated that they exhibit a significant mechanical response to profibrotic factors. Additionally, we conceptually demonstrated the application of microstring chips in constructing METTs with asymmetric, biomimetic constraints. The results indicate effective construction and regulation of METTs, providing a robust platform for mechanobiology and biomedical research.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"1 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142588791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discretised microfluidics for noninvasive health monitoring using sweat sensing† 利用汗液感应进行无创健康监测的离散化微流体技术

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-11-06 DOI: 10.1039/D4LC00763H

Emma J. M. Moonen, Walther Verberne, Eduard Pelssers, Jason Heikenfeld and Jaap M. J. den Toonder

{"title":"Discretised microfluidics for noninvasive health monitoring using sweat sensing†","authors":"Emma J. M. Moonen, Walther Verberne, Eduard Pelssers, Jason Heikenfeld and Jaap M. J. den Toonder","doi":"10.1039/D4LC00763H","DOIUrl":"10.1039/D4LC00763H","url":null,"abstract":"Using sweat instead of blood for monitoring chemical biomarker concentrations of hospitalised patients offers several advantages for both the patients and healthcare workers. Unlike blood, sweat can be noninvasively and continuously sampled without direct involvement of a professional, and sweat contains a rich composition of biomarkers. However, patients in resting state have extremely low sweat rates and they produce correspondingly small sweat volumes, which makes sweat sensing of hospitalised patients highly challenging. We propose a unique solution that enables the use of sweat as a viable biofluid for noninvasive health monitoring, by actively transporting the sweat in a discretised manner. Our device uses electrowetting-on-dielectrics (EWOD) to create and move sweat droplets with a volume of around 1 nanolitre from a sweat gland to sensors integrated in the device. We present the first wearable device with integrated EWOD, and we show that it can collect and transport sweat on-body, while measuring sweat rate, under conditions typical for individuals at rest.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 24","pages":" 5304-5317"},"PeriodicalIF":6.1,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/lc/d4lc00763h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142594926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

5-axis CNC micro-milling machine for three-dimensional microfluidics 用于三维微流体的 5 轴数控微铣床

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-11-04 DOI: 10.1039/d4lc00496e

Mitchell Modarelli, Devin Kot-Thompson, Kazunori Hoshino

{"title":"5-axis CNC micro-milling machine for three-dimensional microfluidics","authors":"Mitchell Modarelli, Devin Kot-Thompson, Kazunori Hoshino","doi":"10.1039/d4lc00496e","DOIUrl":"https://doi.org/10.1039/d4lc00496e","url":null,"abstract":"The gold standard of microfluidic fabrication techniques, SU-8 patterning, requires photolithography equipment and facilities and is not suitable for 3D microfluidics. A 3D printer is more convenient and may achieve high resolutions comparable to conventional photolithography, but only with select materials. Alternatively, 5-axis computer numerical control (CNC) micro-milling machines can efficiently prototype structures with high resolutions, high aspect ratios, and non-planar geometries from a variety of materials. These machines, however, have not been catered for laboratory-based, small-batch microfluidics development and are largely inaccessible to researchers. In this paper, we present a new 5-axis CNC micro-milling machine specifically designed for prototyping 3D microfluidic channels, made affordable for research and laboratories. The machine is assembled from commercially available products and custom-build parts, occupying 0.72 cubic meters, and operating entirely from computer aided design (CAD) and manufacturing (CAM) software. The 5-axis CNC micro-milling machine achieves sub-µm bidirectional repeatability (≤0.23 µm), machinable features <20 µm, and a work volume of 50 x 50 x 68 mm. The tool compatibility and milling parameters were designed to enable fabrication of virtually any mill-able material including metals like aluminum, brass, stainless steel, and titanium alloys. To demonstrate milling high resolution and high aspect ratios, we milled a thin wall from 360 brass with a width of 18.1 µm and an aspect ratio of ~50:1. We also demonstrated fabricating molds from 360 brass with non-planar geometries to create polydimethylsiloxane (PDMS) microfluidic channels. These included a channel on a 90° edge and a channel on a rounded edge with a 250-µm radius of curvature. Our 5-axis CNC micro-milling machine offers the most versatility in prototyping microfluidics by enabling high resolutions, geometric complexity, a large work volume, and broad material compatibility, all within a user-friendly benchtop system.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"214 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142574375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An imaging scheme to study the flow dynamics of co-flow regimes in microfluidics: implications for nanoprecipitation† 研究微流体共流系统流动动态的成像方案：对纳米沉淀的影响

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-10-31 DOI: 10.1039/D4LC00652F

Wali Inam, Anton Vladyka, Joanna W. Pylvänäinen, Junel Solis, Dado Tokic, Pasi Kankaanpää and Hongbo Zhang

{"title":"An imaging scheme to study the flow dynamics of co-flow regimes in microfluidics: implications for nanoprecipitation†","authors":"Wali Inam, Anton Vladyka, Joanna W. Pylvänäinen, Junel Solis, Dado Tokic, Pasi Kankaanpää and Hongbo Zhang","doi":"10.1039/D4LC00652F","DOIUrl":"10.1039/D4LC00652F","url":null,"abstract":"Co-flow microfluidics, in addition to its applications in droplet generation, has gained popularity for use with miscible solvent systems (continuous microfluidics). By leveraging the short diffusional distances in miniature devices, processes like nanomaterial synthesis can be precisely tailored for high-throughput production. In this context, the manipulation of flow regimes—from laminar to vortex formation, as well as the generation of turbulent and turbulent jet flows—plays a significant role in optimizing these processes. Therefore, a detailed understanding of fluid interactions within microchannels is crucial. Imaging with tracer particles is a commonly used approach to study fluid behavior. Alternatively, label-free imaging methodologies are rarely employed for studying fluid dynamics. In this pursuit, we present a new imaging-based scheme to explore fluid interactions in various co-flow regimes through optical flow analysis, specifically using Gaussian window mean squared error (MSE). By examining fluid flow characteristics such as flow intensities (caused by fluctuations) and the projected movement of fluid spots, we characterize slow vortexing and chaotic flow behaviors in co-flow regimes. Consequently, we use imaging data to illustrate the influence of co-flow regimes on particle synthesis. This new tool provides the scientific community with an innovative method to study fluid interactions, which can be further explored to develop a more effective understanding of fluid mixing and optimize fluid manipulation in microfluidic devices.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 24","pages":" 5374-5383"},"PeriodicalIF":6.1,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/lc/d4lc00652f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Deciphering hepatoma cell resistance to tyrosine kinase inhibitors: insights from a Liver-on-a-Chip model unveiling tumor endothelial cell mechanisms 更正：解密肝癌细胞对酪氨酸激酶抑制剂的耐药性：肝芯片模型揭示肿瘤内皮细胞机制的启示。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-10-31 DOI: 10.1039/D4LC90093F

Madhu Shree Poddar, Yu-De Chu, Chau-Ting Yeh and Cheng-Hsien Liu

引用次数: 0

Dual-drive acoustic micromixer for rapid nucleation and ultrafast growth of perovskite nanoparticles† 用于过氧化物纳米粒子快速成核和超快生长的双驱动声学微搅拌器

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-10-30 DOI: 10.1039/D4LC00721B

Zhifang Liu, Yuwen Lu, Wei Tan and Guorui Zhu

{"title":"Dual-drive acoustic micromixer for rapid nucleation and ultrafast growth of perovskite nanoparticles†","authors":"Zhifang Liu, Yuwen Lu, Wei Tan and Guorui Zhu","doi":"10.1039/D4LC00721B","DOIUrl":"10.1039/D4LC00721B","url":null,"abstract":"All-inorganic cesium lead halide perovskites have garnered significant attention owing to their favorable optical properties. Microfluidics-based acoustic mixers are capable of achieving rapid nucleation and ultrafast growth kinetics. Nevertheless, conventional acoustic mixers rely on the response of microstructures to the acoustic field for mixing fluids, the majority of these disturbances occur in the central region of the channel, with minimal impact on the fluid within the side walls. This paper proposes a novel acoustic mixer that combines the effects of sharp corners and bubbles in response to the acoustic field, thereby producing effective disturbance of the fluid throughout the channel. The combined effect enables the micromixer to achieve complete mixing at different inlet flow ratios with mixing times as low as 5 ms. The superiority of acoustic mixers in controlling the nanocrystal formation stage was further validated through the synthesis of chalcogenide nanocrystals using the LARP method. The millisecond mixing time facilitated the rapid formation of nanocrystals and their subsequent rapid growth. The results demonstrate that the green luminescence intensity at 520 nm of the samples synthesized using the acoustic micromixer is 118% higher than that of the samples synthesized using an intermittent reactor. The novel micromixer broadens the range of applications and offers a promising avenue for the large-scale continuous synthesis of high-quality lead-halide perovskite nanocrystals (NCs).","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 1","pages":" 7-15"},"PeriodicalIF":6.1,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Applying low levels of strain to model nascent phenomenon of retinal pathologies† 应用低水平应变来模拟视网膜病变的萌芽现象

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-10-25 DOI: 10.1039/D4LC00205A

Chase Paterson and Elizabeth Vargis

{"title":"Applying low levels of strain to model nascent phenomenon of retinal pathologies†","authors":"Chase Paterson and Elizabeth Vargis","doi":"10.1039/D4LC00205A","DOIUrl":"10.1039/D4LC00205A","url":null,"abstract":"Age-related macular degeneration (AMD) is a leading cause of vision loss in aging populations. A better understanding of the mechanisms of the disease, especially at early stages, could elucidate new treatment targets. One characteristic of AMD is strain on the retinal pigment epithelium (RPE), a crucial layer of the retina. This strain can be caused by physical phenomena like waste aggregation underneath the RPE, drusen formation, or leaky blood vessels that infiltrate the retina during choroidal neovascularization (CNV). It is not well understood how strain affects RPE cell function. Most models generate equibiaxial strain or higher levels of strain that are not representative of early stages of AMD. To overcome these issues, we engineered a device to cause controlled, low amounts of localized, radial strain (maximum ∼1.4%). This strain level is more mimetic to what occurs during aging or at the beginning of physical disruptions experienced during AMD. To evaluate how RPE cells respond to this physical stimulus, primary porcine RPE cells were exposed to low levels of strain applied by our custom-made device. Cell secretions and genetic expression were analyzed to determine how proteins linked to drusen and CNV are affected. The results indicate that this low amount of strain does not immediately initiate angiogenesis but causes changes in mRNA expression of amyloid precursor protein (APP), which plays a role in retinal health and drusen accumulation. This research offers insight into AMD progression as well as the health of other organs, including the brain.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 24","pages":" 5338-5346"},"PeriodicalIF":6.1,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142490256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A gravity-driven tissue chip to study the efficacy and toxicity of cancer therapeutics† 用于研究癌症治疗药物疗效和毒性的重力驱动组织芯片

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-10-24 DOI: 10.1039/D4LC00404C

Pouria Rafsanjani Nejad, Astha Lamichhane, Prasiddha Guragain, Gary Luker and Hossein Tavana

{"title":"A gravity-driven tissue chip to study the efficacy and toxicity of cancer therapeutics†","authors":"Pouria Rafsanjani Nejad, Astha Lamichhane, Prasiddha Guragain, Gary Luker and Hossein Tavana","doi":"10.1039/D4LC00404C","DOIUrl":"10.1039/D4LC00404C","url":null,"abstract":"Tissue chip and organs-on-chip technologies have emerged as promising tools in preclinical studies. In oncology, this is driven by the high failure rates of candidate drugs in clinical trials mainly due to inadequate efficacy or intolerable toxicity and the need for better predictive preclinical models than those traditionally used. However, the intricate design, fabrication, operation, and limited compatibility with automation limit the utility of tissue chips. To tackle these issues, we designed a novel 32-unit tissue chip in the format of standard 96-well plates to streamline automation, fabricated it using 3D printing, and leveraged gravity-driven flow to bypass the need for external flow devices. Each unit includes three interconnected tissue compartments that model liver, tumor, and bone marrow stroma. The focus on liver and bone marrow stroma was due to their respective roles in drug metabolism and disturbances to the bone marrow niche from off-target toxicity of chemotherapies. We analyzed flow patterns, mixing, and oxygen transport among and within the compartments through finite element simulations and demonstrated the utility of the tissue chip to study the efficacy of commonly-used cytotoxic cancer drugs against tumor cells and their toxicity toward liver and bone marrow cells. The ability to simultaneously study drug efficacy and toxicity in high throughput can help select promising therapeutics in early stages of drug discovery in preclinical studies.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 23","pages":" 5251-5263"},"PeriodicalIF":6.1,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/lc/d4lc00404c?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142489901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of microchannel width on axons for brain-on-chip applications† 微通道宽度对脑芯片应用中轴突的影响

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-10-23 DOI: 10.1039/D4LC00440J

Katarina Vulić, Giulia Amos, Tobias Ruff, Revan Kasm, Stephan J. Ihle, Joël Küchler, János Vörös and Sean Weaver

{"title":"Impact of microchannel width on axons for brain-on-chip applications†","authors":"Katarina Vulić, Giulia Amos, Tobias Ruff, Revan Kasm, Stephan J. Ihle, Joël Küchler, János Vörös and Sean Weaver","doi":"10.1039/D4LC00440J","DOIUrl":"10.1039/D4LC00440J","url":null,"abstract":"Technologies for axon guidance for in vitro disease models and bottom up investigations are increasingly being used in neuroscience research. One of the most prevalent patterning methods is using polydimethylsiloxane (PDMS) microstructures due to compatibility with microscopy and electrophysiology which enables systematic tracking of axon development with precision and efficiency. Previous investigations of these guidance platforms have noted axons tend to follow edges and avoid sharp turns; however, the specific impact of spatial constraints remains only partially explored. We investigated the influence of microchannel width beyond a constriction point, as well as the number of available microchannels, on axon growth dynamics. Further, by manipulating the size of micron/submicron-sized PDMS tunnels we investigated the space restriction that prevents growth cone penetration showing that restrictions smaller than 350 nm were sufficient to exclude axons. This research offers insights into the interplay of spatial constraints, axon development, and neural behavior. The findings are important for designing in vitro platforms and in vivo neural interfaces for both fundamental neuroscience and translational applications in rapidly evolving neural implant technologies.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 22","pages":" 5155-5166"},"PeriodicalIF":6.1,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/lc/d4lc00440j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142487387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-actuated microfluidic chiplet for two-stage multiplex nucleic acid amplification assay† 用于两级多重核酸扩增检测的自驱动微流控芯片组

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-10-22 DOI: 10.1039/D4LC00752B

Felix Ansah, Marziyeh Hajialyani, Fatemeh Ahmadi, Yuming Gu, Ergün Alperay Tarım, Michael G. Mauk, Gordon A. Awandare and Haim H. Bau

{"title":"Self-actuated microfluidic chiplet for two-stage multiplex nucleic acid amplification assay†","authors":"Felix Ansah, Marziyeh Hajialyani, Fatemeh Ahmadi, Yuming Gu, Ergün Alperay Tarım, Michael G. Mauk, Gordon A. Awandare and Haim H. Bau","doi":"10.1039/D4LC00752B","DOIUrl":"10.1039/D4LC00752B","url":null,"abstract":"Effective diagnosis of comorbidities and infectious diseases that present similar symptoms requires point-of-need assays capable of co-detecting and differentiating among multiple co-endemic pathogens to enable timely, precision medicine and effective control measures. We previously developed a two-stage isothermal amplification assay dubbed Penn-RAMP to address this need. Penn-RAMP's first stage comprises a recombinase polymerase amplification (RPA), which amplifies all targets of interest in a single reaction chamber for a short duration. The RPA amplicons are then aliquoted into multiple loop-mediated isothermal amplification (LAMP) reaction chambers, each customized with pre-dried primers to amplify a single target or a group of targets. To enable Penn-RAMP at the point of need, we describe here a self-actuated Penn-RAMP chiplet that accommodates the Penn-RAMP assay. Our chiplet employs temperature-controlled phase change valves and capillary valves to self-aliquot first-stage amplicons into multiple (five) second-stage reaction chambers and to seal these chambers. The functionality of our device is demonstrated by co-detecting plant pathogens. The analytical performance of our chiplet is comparable to that of the benchtop Penn-RAMP assay and surpasses that of standalone LAMP assays. Our self-actuated chiplet can be operated standalone with purified nucleic acids or as the downstream amplification module of a sample preparation cassette.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 23","pages":" 5175-5183"},"PeriodicalIF":6.1,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/lc/d4lc00752b?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142486974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0