Lab on a Chip最新文献_第6页

Label-free differentiation of living versus dead single yeast cells using broadband electrical impedance spectroscopy†

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-02-24 DOI: 10.1039/D5LC00043B

Amirhossein Favakeh, Amir Mokhtare, Mohammad Javad Asadi, James C. M. Hwang and Alireza Abbaspourrad

{"title":"Label-free differentiation of living versus dead single yeast cells using broadband electrical impedance spectroscopy†","authors":"Amirhossein Favakeh, Amir Mokhtare, Mohammad Javad Asadi, James C. M. Hwang and Alireza Abbaspourrad","doi":"10.1039/D5LC00043B","DOIUrl":"10.1039/D5LC00043B","url":null,"abstract":"The use of the intrinsic electrical properties of a single cell by broadband electrical impedance spectroscopy (EIS) as a label-free and non-invasive method to monitor cellular and intracellular features is an emerging field. Here, we present a novel EIS-based sheathless microfluidic platform with an integrated coplanar waveguide to probe the interior of a single cell. This platform allows for precise single-cell trapping by dielectrophoresis, hydrodynamic focusing, and sensing the electrical properties of the trapped single cell. We measured the impedance characteristics of a single Schizosaccharomyces pombe (fission) yeast cell by a single frequency sweep (30 kHz to 6GHz) in a stagnant sucrose solution using two-port scattering (S) parameters. The measurements revealed a clear distinction between the cytoplasm impedance of live versus dead cells at 3 GHz. This platform could provide real-time monitoring of cellular electrical responses to chemical and physical antagonists for diagnostic purposes.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 7","pages":" 1744-1754"},"PeriodicalIF":6.1,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d5lc00043b?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pulsatile-flow culture: a novel system for assessing vascular-cell dynamics†

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-02-24 DOI: 10.1039/D4LC00949E

Neda Salimi-Afjani, Robert Rieben and Dominik Obrist

引用次数: 0

Correction: Acoustic modulation and non-contact atomization of droplets based on the Fabry–Pérot resonator

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-02-24 DOI: 10.1039/D5LC90020D

Jingjun Li, Xiukun Wang, Fan Yang, Yadong Sun and Lei Zhang

引用次数: 0

A digital microfluidic approach to increasing sample volume and reducing bead numbers in single molecule array assays† 在单分子阵列检测中增加样品量和减少微珠数量的数字微流体方法。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-02-24 DOI: 10.1039/D4LC01002G

Alinaghi Salari, Jose Gilberto Camacho Valenzuela, Nguyen Le, Joshua Dahmer, Alexandros A. Sklavounos, Cheuk W. Kan, Ryan Manning, David C. Duffy, Nira R. Pollock and Aaron R. Wheeler

{"title":"A digital microfluidic approach to increasing sample volume and reducing bead numbers in single molecule array assays†","authors":"Alinaghi Salari, Jose Gilberto Camacho Valenzuela, Nguyen Le, Joshua Dahmer, Alexandros A. Sklavounos, Cheuk W. Kan, Ryan Manning, David C. Duffy, Nira R. Pollock and Aaron R. Wheeler","doi":"10.1039/D4LC01002G","DOIUrl":"10.1039/D4LC01002G","url":null,"abstract":"We report methods that improve the manipulation of magnetic beads using digital microfluidics (DMF) that can enhance the performance of single molecule array (Simoa) digital protein assays in miniaturized analytical systems. Despite significant clinical and biomedical applications for digital protein detection, the development of miniaturized Simoa systems has been limited by the requirements for use of large sample volumes (∼100 μL) and low numbers of beads (∼5000) for high sensitivity tests. To address these challenges, we improved the integration of DMF with Simoa-based assays by developing strategies for loading mixtures of sample and beads into DMF networks using methods relying on either virtual channels or small liquid segments that were applied either in parallel or in a stepwise manner. We have also demonstrated a dedicated densifying electrode technique that captures low numbers of beads within a droplet, allowing high bead retention with minimal residual volumes of liquid. Based on these improvements, we optimized the front-end assay processing of beads using DMF and demonstrated a method to detect tumor necrosis factor α (TNF-α) by Simoa that showed equivalent performance to a microtitre plate assay. The new strategies described here form a step toward integrating DMF and Simoa for a wide range of applications.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 7","pages":" 1669-1680"},"PeriodicalIF":6.1,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11849296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143481791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensor-integrated gut-on-a-chip for monitoring senescence-mediated changes in the intestinal barrier†

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-02-20 DOI: 10.1039/D4LC00896K

Konstanze Brandauer, Alexandra Lorenz, Silvia Schobesberger, Patrick Schuller, Martin Frauenlob, Sarah Spitz and Peter Ertl

{"title":"Sensor-integrated gut-on-a-chip for monitoring senescence-mediated changes in the intestinal barrier†","authors":"Konstanze Brandauer, Alexandra Lorenz, Silvia Schobesberger, Patrick Schuller, Martin Frauenlob, Sarah Spitz and Peter Ertl","doi":"10.1039/D4LC00896K","DOIUrl":"10.1039/D4LC00896K","url":null,"abstract":"The incidence of inflammatory bowel disease among the elderly has significantly risen in recent years, posing a growing socioeconomic burden to aging societies. Moreover, non-gastrointestinal diseases, also prevalent in this demographic, have been linked to intestinal barrier dysfunction, thus highlighting the importance of investigating aged-mediated changes within the human gut. While gastrointestinal pathology often involves an impaired gut barrier, the impact of aging on the human gastrointestinal barrier function remains unclear. To explore the effect of senescence, a key hallmark of aging, on gut barrier integrity, we established and evaluated an in vitro gut-on-a-chip model tailored to investigate barrier changes by the integration of an impedance sensor. Here, a microfluidic gut-on-a-chip system containing integrated membrane-based electrode microarrays is used to non-invasively monitor epithelial barrier formation and senescence-mediated changes in barrier integrity upon treating Caco-2 cells with 0.8 μg mL−1 doxorubicin (DXR), a chemotherapeutic which induces cell cycle arrest. Results of our microfluidic human gut model reveal a DXR-mediated increase in impedance and cell hypertrophy as well as overexpression of p21, and CCL2, indicative of a senescent phenotype. Combined with the integrated electrodes, monitoring ∼57% of the cultivation area in situ and non-invasively, the developed chip-based senescent-gut model is ideally suited to study age-related malfunctions in barrier integrity.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 7","pages":" 1694-1706"},"PeriodicalIF":6.1,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d4lc00896k?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tutorial on impedance and dielectric spectroscopy for single-cell characterisation on microfluidic platforms: theory, practice, and recent advances

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-02-14 DOI: 10.1039/D4LC00882K

Fatemeh Dadkhah Tehrani, Michael D. O'Toole and David J. Collins

{"title":"Tutorial on impedance and dielectric spectroscopy for single-cell characterisation on microfluidic platforms: theory, practice, and recent advances","authors":"Fatemeh Dadkhah Tehrani, Michael D. O'Toole and David J. Collins","doi":"10.1039/D4LC00882K","DOIUrl":"10.1039/D4LC00882K","url":null,"abstract":"Cell analysis plays an important role in disease diagnosis. However, many characterisation techniques are labour intensive, expensive and time-consuming. Impedance and dielectric spectroscopy (IDS) offers a new approach by using varying electrical current and electric field propagation responses to probe cell physiology. This review aims to explore the theoretical foundations, practical applications, and advancements in IDS for single-cell analysis, particularly when integrated with microfluidic technologies. It highlights recent developments in electrode configurations, calibration techniques, and data analysis methodologies, emphasising their importance in enhancing sensitivity and selectivity. The review identifies key trends, including the shift towards high-throughput and precise single-cell analysis, and discusses the challenges and potential solutions in this field. The implications of these findings suggest significant near-future advances in biomedical research, diagnostics, and therapeutic monitoring. This paper serves as a comprehensive reference for researchers in different fields to make a bridge between theoretical research and practical implementation in single-cell analysis.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 5","pages":" 837-855"},"PeriodicalIF":6.1,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11826307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

iDEP-based single-cell isolation in a two-dimensional array of chambers addressed by easy-to-align wireless electrodes†

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-02-14 DOI: 10.1039/D4LC00976B

Thilini N. Rathnaweera and Robbyn K. Anand

{"title":"iDEP-based single-cell isolation in a two-dimensional array of chambers addressed by easy-to-align wireless electrodes†","authors":"Thilini N. Rathnaweera and Robbyn K. Anand","doi":"10.1039/D4LC00976B","DOIUrl":"10.1039/D4LC00976B","url":null,"abstract":"Platforms capable of selective single-cell capture and enclosure in a fluidically isolated volume for subsequent analysis are crucial for unmasking cellular heterogeneity. Our research group has previously reported an approach that employs wireless bipolar electrodes (BPEs) to facilitate individual isolation of cells in large arrays of pico- to nanoliter scale chambers by dielectrophoresis (DEP). This device was leveraged for a single-cell enzymatic assay and the isolation of circulating tumor cells (CTCs) from patient-derived blood samples, which takes advantage of the selectivity of DEP. However, alignment of BPEs to the microchamber openings is nontrivial, and augmentation of the array dimensions accumulates alignment error, thereby disrupting the uniformity of cell capture across the device. Thus, tolerance-forgiving designs that are simultaneously expandable are in demand. To address this demand, we present an approach that combines BPEs with insulator DEP (iDEP) to drastically expand alignment tolerance. This iDEP-BPE device offers a vertical tolerance (the distance the BPE is recessed within each microchamber) of 80 μm while the horizontal tolerance is nearly infinite. Further, the iDEP-BPE device decreases the exposure of cells to electrode surfaces and reactive oxygen species, thereby preserving their viability. Finally, this iDEP approach can be carried out with BPEs that are easy to fabricate, lacking features that require high-resolution lithography. These advancements potentiate the broad adoption of the iDEP-BPE approach for selective single-cell capture and on-chip analysis and potentiate its commercialization upon deployment of appropriate thermoplastic materials.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 6","pages":" 1600-1610"},"PeriodicalIF":6.1,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11826382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-time and regional analysis of the efficacy of anticancer drugs in a patient-derived intratumoral heterogeneous tumor microenvironment† 在源自患者的瘤内异质肿瘤微环境中对抗癌药物疗效进行实时和区域分析。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-02-14 DOI: 10.1039/D4LC00990H

Ya-Hui Lin, Chiao-Min Lin, Kee-Ming Man, Chih-Chiang Hung, Hsin-Ling Hsu, Yunching Chen, Hsuan-Yu Mu, Tzu-Hung Hsiao and Jen-Huang Huang

{"title":"Real-time and regional analysis of the efficacy of anticancer drugs in a patient-derived intratumoral heterogeneous tumor microenvironment†","authors":"Ya-Hui Lin, Chiao-Min Lin, Kee-Ming Man, Chih-Chiang Hung, Hsin-Ling Hsu, Yunching Chen, Hsuan-Yu Mu, Tzu-Hung Hsiao and Jen-Huang Huang","doi":"10.1039/D4LC00990H","DOIUrl":"10.1039/D4LC00990H","url":null,"abstract":"Preclinical evaluation of anticancer drug efficacy utilizes 2D cell culture systems, tumoroids or experimental animal models, but it suffers from limitations such as inaccurate simulation of tumor microenvironments in living tumors, difficulty in regional analysis, and low throughput. Therefore, in this study, we developed a system named tumor-microenvironment-on-chip (TMoC) comprising a 3D dynamic tumor tissue culture system, which recreated diverse and heterogeneous cellular tumor microenvironments. In addition to the culture with a dynamic circulation, TMoC allowed users to perform real-time regional analysis, independently assessing the drug response from the normoxic area to the hypoxic area in a gradient manner. Through cell composition analysis and gene analysis, we proved that TMoC has a tumor environment with close resemblance to the original tumor environment. By comparing 15 drug testing results with animal experiments, we proved that TMoC is 93% consistent with the response results of animal experiments. In addition, we confirmed that either mouse- or patient-derived tumor cell lines can be cultured and tested in TMoC, indicating its immense potential for all aspects of preclinical drug evaluation.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 7","pages":" 1728-1743"},"PeriodicalIF":6.1,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d4lc00990h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances of dual-organ and multi-organ systems for gut, lung, skin and liver models in absorption and metabolism studies

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-02-13 DOI: 10.1039/D4LC01011F

Konstanze Brandauer, Sophie Schweinitzer, Alexandra Lorenz, Judith Krauß, Silvia Schobesberger, Martin Frauenlob and Peter Ertl

{"title":"Advances of dual-organ and multi-organ systems for gut, lung, skin and liver models in absorption and metabolism studies","authors":"Konstanze Brandauer, Sophie Schweinitzer, Alexandra Lorenz, Judith Krauß, Silvia Schobesberger, Martin Frauenlob and Peter Ertl","doi":"10.1039/D4LC01011F","DOIUrl":"10.1039/D4LC01011F","url":null,"abstract":"Drug development is a costly and timely process with high risks of failure during clinical trials. Although in vitro tissue models have significantly advanced over the years, thus fostering a transition from animal-derived models towards human-derived models, failure rates still remain high. Current cell-based assays are still not able to provide an accurate prediction of the clinical success or failure of a drug candidate. To overcome the limitations of current methods, a variety of microfluidic systems have been developed as powerful tools that are capable of mimicking (micro)physiological conditions more closely by integrating physiological fluid flow conditions, mechanobiological cues and concentration gradients, to name only a few. One major advantage of these biochip-based tissue cultures, however, is their ability to seamlessly connect different organ models, thereby allowing the study of organ-crosstalk and metabolic byproduct effects. This is especially important when assessing absorption, distribution, metabolism, and excretion (ADME) processes of drug candidates, where an interplay between various organs is a prerequisite. In the current review, a number of in vitro models as well as microfluidic dual- and multi-organ systems are summarized with a focus on absorption (skin, lung, gut) and metabolism (liver). Additionally, the advantage of multi-organ chips in identifying a drug's on and off-target toxicity is discussed. Finally, the potential high-throughput implementation and modular chip design of multi-organ-on-a-chip systems within the pharmaceutical industry is highlighted, outlining the necessity of reducing handling complexity.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 6","pages":" 1384-1403"},"PeriodicalIF":6.1,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d4lc01011f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A gut–brain axis on-a-chip platform for drug testing challenged with donepezil†

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-02-12 DOI: 10.1039/D4LC00273C

Francesca Fanizza, Simone Perottoni, Lucia Boeri, Francesca Donnaloja, Francesca Negro, Francesca Pugli, Gianluigi Forloni, Carmen Giordano and Diego Albani

{"title":"A gut–brain axis on-a-chip platform for drug testing challenged with donepezil†","authors":"Francesca Fanizza, Simone Perottoni, Lucia Boeri, Francesca Donnaloja, Francesca Negro, Francesca Pugli, Gianluigi Forloni, Carmen Giordano and Diego Albani","doi":"10.1039/D4LC00273C","DOIUrl":"10.1039/D4LC00273C","url":null,"abstract":"Current drug development pipelines are time-consuming and prone to a significant percentage of failure, partially due to the limited availability of advanced human preclinical models able to better replicate the in vivo complexity of our body. To contribute to an advancement in this field, we developed an in vitro multi-organ-on-a-chip system, that we named PEGASO platform, which enables the dynamic culturing of human cell-based models relevant for drug testing. The PEGASO platform is composed of five independent connected units, which are based on a previously developed millifluidic organ-on-a-chip device (MINERVA 2.0), hosting human primary cells and iPSC-derived cells recapitulating key biological features of the gut, immune system, liver, blood–brain-barrier and brain that were fluidically connected and challenged to model the physiological passage of donepezil, a drug prescribed for Alzheimer's disease. The nutrient medium flow rate of the connected units was tuned to obtain suitable oxygenation and shear stress values for the cells cultured in dynamic condition. A computational model was at first developed to simulate donepezil transport within the platform and to assess the drug amount reaching the last organ-on-a-chip. Then, we demonstrated that after 24 hours of donepezil administration, the drug was actually transported though the cell-based models of the platform which in turn were found viable and functional. Donepezil efficacy was confirmed by the decreased acetylcholinesterase activity at the brain model and by the increased expression of a donepezil-relevant multi-drug transporter (P-gp). Overall, the PEGASO platform is an innovative in vitro tool for drug screening and personalized medicine applications which holds the potential to be translated to preclinical research and improve new drug development pipelines.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 7","pages":" 1854-1874"},"PeriodicalIF":6.1,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d4lc00273c?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0