Lab on a Chip最新文献_第6页

Open-space microfluidics as a tool to study signaling dynamics 开放空间微流体作为研究信号动力学的工具

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-09-18 DOI: 10.1039/D5LC00521C

Maude Proulx, Pierre Clapperton-Richard, Laurent Potvin-Trottier, Alisa Piekny and Thomas Gervais

{"title":"Open-space microfluidics as a tool to study signaling dynamics","authors":"Maude Proulx, Pierre Clapperton-Richard, Laurent Potvin-Trottier, Alisa Piekny and Thomas Gervais","doi":"10.1039/D5LC00521C","DOIUrl":"10.1039/D5LC00521C","url":null,"abstract":"The temporal dynamics of cell signaling are a crucial way for cells to regulate their transcriptional targets and consequently may heavily influence cell responses. Improving our understanding of signaling dynamics is important for drug treatments targeting specific signaling pathways. However, studying signaling dynamics requires multiplexed, time-sensitive experiments. Here, we use an open-space microfluidic device, the microfluidic display, which enables liquid delivery from above a surface, forming defined and stable confinement zones without enclosing samples into a chip. A device with rapid reagent switching (<7 seconds) and 6 independent confinement areas is first designed. Using this platform, we study the Notch pathway in engineered C2C12 cells to display constitutively active Notch receptors upon which we force highly controlled time-dependent modulation patterns by delivering time-varying doses of the Notch inhibitor DAPT. We replicate previous findings on Notch activation with our methodology by confirming the Notch-regulated gene Hes1 is upregulated for short Notch activation pulses, while Hey1 required sustained activation. We confirm a previously observed regime switch from Hes1 to Hey1 dominance between 2 h and 3 h of activation. Finally, by varying signal pulses while keeping dose constant in six independent experiments performed simultaneously, we further show the upregulation of the Hes1 gene for multiple short pulses, while Hey1 activation depends on duty cycle length. These results highlight microfluidic displays as a valuable tool for systems biology, enabling multiplexed, high temporal resolution stimulation of signaling pathways.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 21","pages":" 5592-5605"},"PeriodicalIF":5.4,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d5lc00521c?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145077684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Barcode-integrated cellulose based microfluidic system for intelligent point-of-care blood typing 基于纤维素的条形码集成微流体系统，用于智能护理点血型

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-09-18 DOI: 10.1039/D5LC00688K

Chunrui Chen, Tonghuan Zhan, Lin Hu, Mengyuan Ding, Xianchang Wu, Heng Wang and Bing Xu

{"title":"Barcode-integrated cellulose based microfluidic system for intelligent point-of-care blood typing","authors":"Chunrui Chen, Tonghuan Zhan, Lin Hu, Mengyuan Ding, Xianchang Wu, Heng Wang and Bing Xu","doi":"10.1039/D5LC00688K","DOIUrl":"10.1039/D5LC00688K","url":null,"abstract":"Accurate and rapid blood typing plays a critical role in life-saving clinical procedures such as blood transfusions and organ transplantation. Herein, we proposed a novel blood typing system (BloodStrips) that combines cellulose based microfluidics with universal barcode technology, achieving intelligent, rapid, and user-friendly blood type detection. The BloodStrips system employed heat transfer printing to create barcode patterns on hydrophobic cotton substrates and integrated cotton threads to construct hydrophilic channels. Meanwhile, the swinging elution method was harnessed to remove free red blood cells (RBCs) while retaining aggregated RBCs on the cotton threads, thereby resulting in creating a distinct white/red contrast at the macro level (white represents cotton thread, red represents bloodstain). The white/red barcodes with different combinations were used to represent various blood types. Based on this principle, we further developed a portable and automated blood tying chip called the BloodBar chip. It is worth noting that this device leverages a simple and straightforward smartphone scanning technique to decipher blood types, avoiding reading errors caused by ambient light intensity and personal bias. This work provides a universal and intelligent visual diagnostic platform for simple, rapid, and accurate blood typing, which may find wide applications in developing countries or resource-limited areas.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 21","pages":" 5628-5637"},"PeriodicalIF":5.4,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145077686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stress-actuated partially flexible microelectrode arrays for activity recording in 3D neuronal cultures 用于三维神经元培养活动记录的应力驱动部分柔性微电极阵列

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-09-17 DOI: 10.1039/D4LC01077A

João Serra, José C. Mateus, Susana Cardoso, João Ventura, Paulo Aguiar and Diana C. Leitao

{"title":"Stress-actuated partially flexible microelectrode arrays for activity recording in 3D neuronal cultures","authors":"João Serra, José C. Mateus, Susana Cardoso, João Ventura, Paulo Aguiar and Diana C. Leitao","doi":"10.1039/D4LC01077A","DOIUrl":"10.1039/D4LC01077A","url":null,"abstract":"Microelectrode arrays (MEAs) are instrumental in monitoring electrogenic cell populations, such as neuronal cultures, allowing high precision measurements of electrical activity. Although three-dimensional neuronal cultures replicate the behavior of in vivo systems better than two-dimensional models, conventional planar MEAs are not well suited to capture activity within such networks. Novel MEA geometries can overcome this difficulty, but often at the cost of increased fabrication complexity. Here, we used the stress mismatch between thin film layers to fabricate MEAs with vertical electrodes, using methods compatible with established microfabrication protocols. A micrometric SiO2 hinge enables control over the bending angle of flexible polyimide structures with embedded electrodes. The performance of the patterned electrodes was assessed before and after stress actuation, through impedance measurements, voltage noise mapping, and neuronal activity recordings. 3D MEAs with 30 × 30 μm2 electrodes showed an impedance of 0.96 ± 0.07 MΩ per electrode and detected neuronal activity spikes with amplitudes as high as 400 μV. These results demonstrate the potential of the developed methods to provide a scalable approach to fabricate 3D MEAs, enabling enhanced recording capabilities for in vitro neuronal cultures.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 21","pages":" 5574-5583"},"PeriodicalIF":5.4,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145072319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A biocompatible surfactant film for stable microfluidic droplets 用于稳定微流体液滴的生物相容性表面活性剂膜

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-09-17 DOI: 10.1039/D5LC00456J

Brendan T. Deveney, John A. Heyman, Raoul G. Rosenthal, David A. Weitz and Jörg G. Werner

引用次数: 0

Optimizing microfluidic flow cell geometry for in situ resonant soft X-ray characterization of molecular nanostructures 优化微流体流动池几何结构用于分子纳米结构的原位共振软x射线表征

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-09-16 DOI: 10.1039/D5LC00765H

Devin Grabner, Terry McAfee, Cheng Wang, Matthew A. Marcus and Brian A. Collins

{"title":"Optimizing microfluidic flow cell geometry for in situ resonant soft X-ray characterization of molecular nanostructures","authors":"Devin Grabner, Terry McAfee, Cheng Wang, Matthew A. Marcus and Brian A. Collins","doi":"10.1039/D5LC00765H","DOIUrl":"10.1039/D5LC00765H","url":null,"abstract":"Liquid-phase resonant soft X-ray scattering (LP-RSoXS) is an emerging label-free technique to probe chemically resolved nanostructures of molecular or hybrid materials in liquid environments. Still, quantitative analysis is hindered by the pressure-induced deformation of thin silicon nitride (SiN) membranes used as windows in microfluidic flow cells, which attenuates the signal in nonlinear ways, making experimental optimization difficult. Here, we directly characterize this deformation under experimental conditions for a variety of cell configurations. We use this to develop a predictive model that combines transmission effects of SiN bowing, incident X-ray beam profiles, and material-dependent resonant scattering cross sections to simulate the effective scattering intensity at the detector across the carbon K-edge. Maps of the total signal across the flow cell window reveal that increasing the window width and polymer concentration shifts the anisotropic intensity distributions from the center toward the edges of the window. It was determined that an optimal SiN thickness of 50 nm, with a window aperture of 104 μm, maximizes the total signal for typical solute concentrations and energies across the carbon K-edge. Our results overturn the assumption that corner regions dominate the scattering signal, offering explicit design guidelines for maximizing LP-RSoXS signals and significantly advancing the quantitative application of this technique to the characterization of molecular and hybrid nanostructured materials in liquids.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 21","pages":" 5584-5591"},"PeriodicalIF":5.4,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145072321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Candyfluidics: the art of fabricating micro- and nano-fluidic geometries using surface-deposited sugar scaffolds 糖流体：利用表面沉积的糖支架制造微和纳米流体几何形状的艺术

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-09-16 DOI: 10.1039/D5LC00710K

Tochukwu Dubem Anyaduba and Jesus Rodriguez-Manzano

引用次数: 0

Modular bioreactor for multi-well electrical stimulation of in vitro cardiac tissue engineering constructs 用于体外心脏组织工程多孔电刺激的模块化生物反应器

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-09-15 DOI: 10.1039/d5lc00234f

Suh Hee Cook, Jack Twiddy, Yuan Li, Kiran M. Ali, Daxian Zha, Kaleah Gaddy, Lauren Mabe, Ke Huang, Ke Cheng, Michael Daniele, Jessica M Gluck

{"title":"Modular bioreactor for multi-well electrical stimulation of in vitro cardiac tissue engineering constructs","authors":"Suh Hee Cook, Jack Twiddy, Yuan Li, Kiran M. Ali, Daxian Zha, Kaleah Gaddy, Lauren Mabe, Ke Huang, Ke Cheng, Michael Daniele, Jessica M Gluck","doi":"10.1039/d5lc00234f","DOIUrl":"https://doi.org/10.1039/d5lc00234f","url":null,"abstract":"Systems for providing electrical stimulation to in vitro cell cultures are valuable in many tissue engineering applications. We designed and fabricated a novel modular bioreactor consisting of a printed circuit board assembly and carbon paper electrodes which is compatible with commercially available 12-well plates. Our system is the first of its kind and capable of simultaneously supplying four different amplitudes of stimulus waveform to different wells of the bioreactor. SPICE and FEA were used to model and validate the delivery of these stimuli to cells in culture. We then used our bioreactor to apply 0 V, 0.1 V, 1 V, and 10 V of electrical stimulation to neonatal rat cardiomyocytes (NRCMs), with and without neonatal rat ventricular fibroblast co-culture, for 10 minutes daily on 7 consecutive days. Electrically stimulated NRCMs maintained viability except in response to 10 V stimulation in the absence of fibroblast co-culture. Furthermore, NRCMs exposed to 0.1 V stimulation exhibited enhanced markers (sarcomeric α-actinin and connexin 43) and upregulated genes (βMYC, cTnI, Cav1, and Cx43) related to cardiac electrophysiology compared to non-stimulated controls. This suggests 0.1 V stimulation with our bioreactor is advantageous for the electrophysiological function of primary cardiac cells; it may also be useful in electrophysiologically maturing induced pluripotent stem cell-derived cardiomyocytes.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"104 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145059484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Secondary vortex induced by gigahertz acoustic streaming and its applications for 3D particle manipulation 千兆赫声流诱导二次涡及其在三维粒子操纵中的应用

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-09-15 DOI: 10.1039/D5LC00640F

Yangchao Zhou, Haitao Zhang, Wenlan Guo, Chen Sun and Xuexin Duan

{"title":"Secondary vortex induced by gigahertz acoustic streaming and its applications for 3D particle manipulation","authors":"Yangchao Zhou, Haitao Zhang, Wenlan Guo, Chen Sun and Xuexin Duan","doi":"10.1039/D5LC00640F","DOIUrl":"10.1039/D5LC00640F","url":null,"abstract":"Acoustic streaming serves as a fast-developing contactless tool for manipulation of micro/nanoparticles. However, traditional Eckart streaming has low manipulation precision and Rayleigh streaming only works in a very close distance to the solid boundaries, which limits their applications over a large spatial distance in fluid. In this paper, we propose a novel boundary-independent secondary vortex induced by the high frequency (GHz) acoustic streaming and demonstrate its application for precise three-dimensional (3D) particle manipulation. The size of the Eckart streaming generated by the micro-fabricated acoustic transducer can be efficiently compressed by increasing the viscosity of the solution, which results in a more stable and controllable secondary convection streaming in the chamber. Arbitrary and precise manipulation of different types of particles in 3D space is approached by controlling the position and power of the transducer. Moreover, multilayer assembly of cell-encapsulating hydrogels is achieved to validate the high throughput and good biocompatibility of the secondary vortex. This secondary vortex-based manipulation tool exhibits versatility toward different objects, highly efficient assembly, and good biocompatibility for handling viable samples, and shows potential in bio-fabrication, material synthesis, tissue engineering, etc.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 21","pages":" 5450-5459"},"PeriodicalIF":5.4,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145059482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interplay between dietary fiber, macrophages and colonocytes in a microfluidic model of host-microbiota interactions in colorectal cancer 膳食纤维、巨噬细胞和结肠细胞在大肠癌中宿主-微生物相互作用的微流控模型中的相互作用

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-09-11 DOI: 10.1039/D5LC00052A

Daniel Penarete-Acosta, Mohet Mittal, Sanjukta Chakraborty, Arum Han and Arul Jayaraman

{"title":"Interplay between dietary fiber, macrophages and colonocytes in a microfluidic model of host-microbiota interactions in colorectal cancer","authors":"Daniel Penarete-Acosta, Mohet Mittal, Sanjukta Chakraborty, Arum Han and Arul Jayaraman","doi":"10.1039/D5LC00052A","DOIUrl":"10.1039/D5LC00052A","url":null,"abstract":"Dietary fiber has been consistently associated with a decreased risk of colorectal cancer development. While the apoptotic effect of dietary fiber microbial fermentation products, such as short chain fatty acids on tumor colonocytes, is well established, the role of these products on other components of the tumor microenvironment remains unexplored. Tumor associated macrophages play a critical role in tumor development in colorectal cancer; however, the effect of dietary fiber fermentation by microbiota on the interaction between macrophages and colonocytes in the colorectal cancer microenvironment has been difficult to dissect due to a lack of in vitro models of colorectal cancer containing immune cells, colonocytes, and microbiota. Recently, we developed a microfluidic model that facilitates the coculture of colorectal cancer spheroids with complex microbial communities. Here, we expand our model to include macrophages and employ it to study the impact of dietary fiber on macrophage-colonocyte interaction. We optimized monocyte differentiation parameters in vitro and demonstrated the capacity of our model to recapitulate changes in microbiota composition and metabolic output associated with dietary fiber administration in vivo. Coculture of colonocytes with microbiota and macrophages revealed that alterations in microbial production of short chain fatty acids derived from dietary fiber fermentation correlated with decreased colonocyte viability, possibly mediated by an increase in production of tumor pro-apoptotic cytokines by macrophages. Our work highlights the capacity of microfluidic in vitro models to study the role of microbial metabolism of dietary molecules on colorectal cancer colonocyte viability in the presence of macrophages.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 21","pages":" 5482-5496"},"PeriodicalIF":5.4,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d5lc00052a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145035678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micro elastofluidic liquid diode for programmable unidirectional flow control 用于可编程单向流量控制的微弹性流体二极管

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-09-11 DOI: 10.1039/D5LC00438A

Haotian Cha, Fariba Malekpour Galogahi, Quang Thang Trinh, Sharda Yadav, Jun Zhang, Hongjie An, Qin Li and Nam-Trung Nguyen

{"title":"Micro elastofluidic liquid diode for programmable unidirectional flow control","authors":"Haotian Cha, Fariba Malekpour Galogahi, Quang Thang Trinh, Sharda Yadav, Jun Zhang, Hongjie An, Qin Li and Nam-Trung Nguyen","doi":"10.1039/D5LC00438A","DOIUrl":"10.1039/D5LC00438A","url":null,"abstract":"Controllable liquid transport is essential for fluid regulation in wearable biosensing platforms. Particularly, unidirectional flow offers a passive, geometry-dependent strategy to direct liquid movement without external actuation. However, most previous studies have focused solely on achieving unidirectional flow, with limited exploration of real-time tunability or reconfigurability. Here, we present a tuneable open-channel microfluidic platform featuring a chevron–ratchet geometry that enables passive and reversible liquid diode behaviour. Flow directionality and velocity are dynamically modulated through surface wettability tuning and mechanical stretching. A theoretical force model was first established to describe asymmetrical spreading, governed by Laplace pressure gradients and geometric curvature. Numerical simulations based on energy-minimization principles further elucidated wetting behaviour on structured surfaces. Concurrently, experimental validation confirmed three distinct flow regimes—pinned, unidirectional, and bidirectional—controlled by plasma-induced wettability modulation and applied mechanical strain. Stretching the channels along orthogonal axes led to programmable switching of flow states and geometry-sensitive pinning thresholds. We further integrated a hydrogel film as a sweat-acquisition interface and demonstrated sustained unidirectional transport under physiologically relevant inflow. This proof-of-concept validation complements the fundamental findings and highlights the translational potential of our open-channel platform as a simple, tuneable, and pumpless approach for wearable diagnostics, adaptive liquid routing, and flexible microfluidic circuitry.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 21","pages":" 5460-5472"},"PeriodicalIF":5.4,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145035658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0