采用微流控过滤技术对高岭石微/纳米片进行粒径选择性分选的创面止血研究

IF 5.4 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS
Lab on a Chip Pub Date : 2025-06-06 DOI:10.1039/D5LC00274E
Guangyao Li, Liang Wan, Ying Chen, Xuming Zhang, Aidong Tang and Huaming Yang
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引用次数: 0

摘要

高岭石是一种天然的微纳米粘土材料,具有显著的伤口止血作用,但其效果受到颗粒大小不均的严重限制。因此,基于大小差异的排序对于提高其性能至关重要。然而,与球形或椭圆形材料相比,具有层状结构的高岭石在分选方面存在挑战,其尺寸分布范围从纳米到微米不等,这对精确分选提出了重大挑战。因此,我们开发了一种双层微流控过滤芯片,以实现高岭石微/纳米片(尺寸为1.582 ~ 0.377 μm)的高通量分选。分级孔径的双层过滤膜结构可对特定粒径范围内的高岭石颗粒进行选择性分选,并采用共流流体排列方式缓解膜堵塞。通过体内和体外实验对不同大小的高岭石颗粒的止血性能进行了评价,揭示了高岭石对伤口止血的显著的尺寸依赖性作用。不同粒径高岭石在凝血过程中的作用机理,特别是对血小板活化和凝血因子活化的影响,为优化高岭石基止血材料提供了理论依据。这项工作建立了一种可扩展的微流体策略,用于精确分选片状纳米材料,并提高了高岭石在急诊伤口止血中的转化潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Size-selective sorting of kaolinite micro/nanoflakes via microfluidic filtration for wound hemostasis†

Size-selective sorting of kaolinite micro/nanoflakes via microfluidic filtration for wound hemostasis†

Kaolinite, a natural micro/nano clay material, exhibits remarkable effect on wound hemostasis, yet its efficacy is critically limited by heterogeneous particle sizes. Therefore, sorting based on size differences is essential to improve its performance. However, kaolinite with a layer structure presents challenges in sorting compared to spherical or elliptical materials, and the size distribution ranges continuously from nanometers to micrometers, which poses significant challenges for precise sorting. Hence, we developed a dual-layer microfluidic filtration chip, to enable high-throughput sorting of kaolinite micro/nanoflakes (size from 1.582 to 0.377 μm). The dual-layer filter membrane structure with graded pore sizes enabled selective sorting of kaolinite particles within a specific size range, and the co-flow fluid arrangement was employed to alleviate membrane clogging. The hemostatic properties of kaolinite particles with different sizes were evaluated through in vivo and in vitro experiments, revealing the significant size-dependent effects of kaolinite on wound hemostasis. The mechanism of different sizes of kaolinite in the process of coagulation, especially the effect on platelet activation and coagulation factor activation, provided a theoretical basis for optimizing kaolinite-based hemostatic materials. This work established a scalable microfluidic strategy for precise sorting of sheet nanomaterials and improved the translational potential of kaolinite in emergency wound hemostasis.

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来源期刊
Lab on a Chip
Lab on a Chip 工程技术-化学综合
CiteScore
11.10
自引率
8.20%
发文量
434
审稿时长
2.6 months
期刊介绍: Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.
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