Oriana Gerallin Chavez-Pineda, Pablo E. Guevara-Pantoja, Victor Marin, Gabriel Arturo Caballero-Robledo, Luis David Patino-Lopez, Daniel A May-Arrioja, Clelia De-la-Peña, Jose L. Garcia-Cordero
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Parallel DLD Microfluidics for Chloroplast Isolation and Sorting
Chloroplasts are characteristic organelles of plant cells, essential for photosynthesis and various other metabolic processes, including amino acid, lipid, and hormone biosynthesis. Beyond their classical functions, chloroplasts have emerged as promising targets in biotechnology, particularly in therapeutic applications and biofuel production. However, their isolation remains technically challenging due to the limitations of conventional methods, which typically require complex protocols, specialized equipment, and trained personnel. Here, we present a microfluidic-based platform that enables size-based chloroplast separation using deterministic lateral displacement (DLD). Our device integrates four parallel DLD arrays, each with a distinct critical diameter (CD). This configuration enables bandpass filtering and allows the simultaneous isolation of chloroplasts of various sizes within a single device. Shared inlets and uniform flow conditions across all arrays enhance reproducibility compared to conventional techniques. Unlike traditional sucrose density gradients, which lack precise size-based separation, our system achieves separation efficiencies of 50-85% for chloroplasts ranging from 3 to 8 µm, with recovered fractions having purities of 17-66%. This platform provides a rapid, automated, and scalable solution for chloroplast isolation, with significant potential applications in plant research, biotechnology, and synthetic biology.
期刊介绍:
Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.