A multi-channel chip enabled synchronized reciprocating-flow of fluid for rapid, simultaneous, multiplex detection of inflammatory markers.

IF 6.1 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS
Lab on a Chip Pub Date : 2025-06-09 DOI:10.1039/d5lc00132c
Juanhua Li, Zihan Xiao, Tianyu Wu, Yiren Liu, Wenyong Zhang, Cuiping Zhou, Yanqiong Su, Hongrui Liang, Donglin Cao, Jianhua Zhou
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引用次数: 0

Abstract

Infections are common in daily life and can lead to various acute diseases with complex symptoms. Rapid and accurate detection of multiple inflammatory markers using multi-channel microfluidic chips provides important guidance for controlling the development of infections. However, current fluid control methods for multi-channel microfluidic chips typically involve controlling the liquid in each channel separately and successively, which increases the complexity of control operations and the detection time. Here, we proposed a circularly-aligned parallel channel chip (CAPC chip) with multiple channels and used this chip for the rapid, simultaneous detection of four inflammatory markers. We implemented the integrated fluid control method and achieved a synchronized reciprocating-flow of fluid and the precise control of the flow velocity of fluid in multiple channels using a single air-source pressure control device. Then, we used the CAPC chip to perform rapid, simultaneous enzyme-linked immunosorbent assay (ELISA) detection of four inflammatory markers (CRP, PCT, IL-6, SAA). The results showed that the CAPC chip can complete qualitative and quantitative detection of these four inflammatory markers within 5 min, which is the fastest ELISA detection for simultaneous detection of multiple protein biomarkers so far. Furthermore, we used the CAPC chip to detect these four inflammatory markers in simulated serum samples and provide prompt information regarding the classification and severity of infection. These findings demonstrate the potential of the CAPC chip for rapid, simultaneous, multiplex immunoassays.

多通道芯片使流体同步往复流动,用于快速、同时、多重检测炎症标志物。
感染在日常生活中很常见,可导致各种症状复杂的急性疾病。利用多通道微流控芯片快速准确地检测多种炎症标志物,为控制感染的发展提供了重要的指导。然而,目前多通道微流控芯片的流体控制方法通常是对每个通道中的液体分别、先后进行控制,这增加了控制操作的复杂性和检测时间。在这里,我们提出了一种具有多个通道的圆对齐平行通道芯片(CAPC芯片),并使用该芯片快速,同时检测四种炎症标志物。采用一体化流体控制方法,利用单一气源压力控制装置实现了流体的同步往复流动和多通道流体流速的精确控制。然后,我们使用CAPC芯片进行快速,同时酶联免疫吸附试验(ELISA)检测四种炎症标志物(CRP, PCT, IL-6, SAA)。结果表明,CAPC芯片可在5 min内完成对这4种炎症标志物的定性和定量检测,是目前最快的同时检测多种蛋白生物标志物的ELISA检测。此外,我们使用CAPC芯片检测模拟血清样本中的这四种炎症标志物,并及时提供有关感染分类和严重程度的信息。这些发现证明了CAPC芯片在快速、同时、多重免疫分析方面的潜力。
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来源期刊
Lab on a Chip
Lab on a Chip 工程技术-化学综合
CiteScore
11.10
自引率
8.20%
发文量
434
审稿时长
2.6 months
期刊介绍: Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.
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