Tochukwu Dubem Anyaduba and Jesus Rodriguez-Manzano
{"title":"Candyfluidics: the art of fabricating micro- and nano-fluidic geometries using surface-deposited sugar scaffolds","authors":"Tochukwu Dubem Anyaduba and Jesus Rodriguez-Manzano","doi":"10.1039/D5LC00710K","DOIUrl":null,"url":null,"abstract":"<p >We present Candyfluidics, a rapid and low-cost method for fabricating micro- and nanofluidic devices using sugar mixtures patterned by screen-printing. The process (from screen preparation to PDMS casting) takes less than 30 minutes, supports parallel production of multiple chips, and exploits household materials and simple technology widely available in low-resource regions. As a proof of concept, we fabricated flow-focusing chips and validated them by generating pressure-driven water-in-oil droplets with volumes from 0.2 to 1.22 nL. We further demonstrated the utility of the fabricated chips by performing digital droplet loop-mediated isothermal amplification to detect dengue virus type 1 nucleic acids at femtomolar concentrations (85 copies per μL). By lowering the cost and technical barriers to device prototyping, Candyfluidics offers an accessible approach to microfluidic manufacturing with potential for point-of-need applications for global health interventions.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 21","pages":" 5606-5616"},"PeriodicalIF":5.4000,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d5lc00710k?page=search","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lab on a Chip","FirstCategoryId":"5","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2025/lc/d5lc00710k","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
We present Candyfluidics, a rapid and low-cost method for fabricating micro- and nanofluidic devices using sugar mixtures patterned by screen-printing. The process (from screen preparation to PDMS casting) takes less than 30 minutes, supports parallel production of multiple chips, and exploits household materials and simple technology widely available in low-resource regions. As a proof of concept, we fabricated flow-focusing chips and validated them by generating pressure-driven water-in-oil droplets with volumes from 0.2 to 1.22 nL. We further demonstrated the utility of the fabricated chips by performing digital droplet loop-mediated isothermal amplification to detect dengue virus type 1 nucleic acids at femtomolar concentrations (85 copies per μL). By lowering the cost and technical barriers to device prototyping, Candyfluidics offers an accessible approach to microfluidic manufacturing with potential for point-of-need applications for global health interventions.
期刊介绍：
Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.
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