A novel holder and microfluidic system for spatially controlled hypoxia induction, mechanical stimulation and cardiac regeneration research

IF 5.4 2区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS

Lab on a Chip Pub Date : 2025-09-18 DOI:10.1039/d5lc00460h

Kołodziejek Dominik, Szlachetka Aleksandra, Iwoń-Szczawińska Zuzanna, Drozd Marcin, Wojasiński Michał, Jastrzębska Elżbieta

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Abstract

Over the years, cardiovascular diseases have remained a leading cause of mortality worldwide, necessitating advanced experimental models to mimic the natural environment of the heart as closely as possible. New microfluidic heart models would enable precise modelling of the healthy heart as well as pathological mechanisms occurring during cardiac ischemia and testing new therapies would become more accessible than it is currently. In this study, we developed a two layer holder for creating hypoxic conditions in a dedicated microfluidic system for modelling cardiac infarction. By integrating the holder with a microsystem utilizing nanofibrous mats with iron(II, III) oxide (Fe₃O₄) magnetic nanoparticles we were able to create a tool for using (potentially patient specific) induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) in cardiac research. In the microsystem we induced hypoxia and conducted preliminary observations of cardiac regeneration using induced pluripotent stem cells (iPSCs). We found that iPSCs express cTnT when co-cultured with hypoxia-damaged iPSC-CMs indicating that the holder with an integrated microsystem can be used for hypoxia and cardiac regeneration studies.

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一种用于空间控制缺氧诱导、机械刺激和心脏再生研究的新型支架和微流体系统

多年来，心血管疾病一直是世界范围内死亡的主要原因，需要先进的实验模型来尽可能地模拟心脏的自然环境。新的微流体心脏模型将能够精确地模拟健康心脏以及心脏缺血期间发生的病理机制，并且测试新的治疗方法将比目前更容易获得。在这项研究中，我们开发了一种两层支架，用于在专用的微流体系统中创建缺氧条件，用于模拟心肌梗死。通过将支架与带有铁（II， III）氧化物（Fe3O4）磁性纳米颗粒的纳米纤维垫的微系统集成，我们能够创建一个工具，用于（潜在的患者特异性）诱导多能干细胞衍生的心肌细胞（iPSC-CMs）在心脏研究中。在微系统中，我们诱导缺氧，并使用诱导多能干细胞（iPSCs）对心脏再生进行了初步观察。我们发现iPSCs与缺氧损伤的iPSC-CMs共培养时表达cTnT，这表明具有集成微系统的支架可用于缺氧和心脏再生研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Lab on a Chip 工程技术-化学综合

CiteScore

11.10

自引率

8.20%

发文量

434

审稿时长

2.6 months

期刊介绍： Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.