Biosensors and Bioelectronics最新文献

{"title":"A miniaturized electrochemical biosensor for real-time in vivo potassium monitoring in the inner ear","authors":"Faezeh Arshadi ,&nbsp;Mahsa Esfandiari ,&nbsp;Robert Gay ,&nbsp;Ya Lang Enke ,&nbsp;Nona Farbehi ,&nbsp;Christopher Pastras ,&nbsp;Mohsen Asadnia","doi":"10.1016/j.bios.2026.118388","DOIUrl":"10.1016/j.bios.2026.118388","url":null,"abstract":"<div><div>The ability to continuously monitor key biomarkers is crucial for advancing personalized medicine and enabling early disease intervention. Potassium ions (K⁺) play a vital role in neural signaling, cardiac function, and sensory processing, particularly in the cochlea, where potassium imbalances are linked to disorders such as Ménière's disease. However, existing diagnostic methods cannot monitor K⁺ in the inner ear in real time, limiting insight into the ionic mechanisms that underlie auditory function and related disorders. This study presents a miniaturized potassium-selective biosensor, incorporating a stable reference electrode, for continuous and real-time monitoring of K⁺ dynamics in the inner ear. The sensor underwent extensive benchtop validation, demonstrating high sensitivity (⁓52.8 mV/dec), a broad linear range (10⁻⁵–10⁻¹ mol/L), a limit of detection of 10⁻⁵·¹⁶ mol/L, strong selectivity coefficients (−2.62 for Na⁺, −4.10 for Mg²⁺, and −3.85 for Ca²⁺), and excellent stability over two months. In-vivo experiments in a guinea pig model confirmed the biosensor's capability to track dynamic potassium fluctuations under physiological conditions, such as responses to controlled potassium administration. These findings establish the feasibility of real-time K⁺ monitoring in the cochlea, providing a valuable tool for studying auditory physiology, understanding potassium regulation in the inner ear, and advancing targeted diagnostics and treatments for inner ear disorders.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118388"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146008073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfluidic device for continuous blood plasma separation from whole blood","authors":"Surendran Velmurugan ,&nbsp;Melis Güler Girbas ,&nbsp;Can Dincer ,&nbsp;Meltem Avci-Adali ,&nbsp;Stefan Partel","doi":"10.1016/j.bios.2026.118404","DOIUrl":"10.1016/j.bios.2026.118404","url":null,"abstract":"<div><div>Sample preparation is a critical step in clinical diagnostics, as plasma must be separated from whole blood before analysis. Conventional centrifugation is time-consuming, require bulky equipment, and is unsuitable for point-of-care (POC) applications. Here, we present a simple and low-cost microfluidic device that achieves continuous (uninterrupted flow separation without batch processing) and real-time (immediate flow separation and inline without delays) plasma separation from undiluted whole human blood without external forces. Six channel geometries were evaluated by numerical simulations to investigate the effects of side-channel orientation and channel height on red blood cell (RBC) contamination carryover. The optimized configuration with a 20-μm channel height, minimized recirculation zones and enhanced inertial focusing, leading to reduced RBC contamination in the side channel plasma outlet. Devices were then fabricated by standard photolithography and soft lithography. Experimental validation with whole human blood demonstrated that the device significantly reduced red blood cell (RBC) contamination across a flow rate range of 0.05–0.2 mL min⁻¹, yielding plasma with substantially lower RBC levels compared with whole blood, although low residual RBCs were still present. Flow cytometry further confirmed an efficient depletion of peripheral blood mononuclear cells (PBMCs), resulting in markedly lower leukocyte content than in plasma obtained by standard centrifugation. The plasma yield reached 3.8 %, corresponding to an extraction rate of 6.5 μL min⁻¹, and hemolysis levels remained comparable to centrifuged samples, indicating preservation of plasma integrity. This established device provides a robust platform for high-purity plasma separation and has a strong potential for point-of-care diagnostic applications.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118404"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146008121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine-learning-optimized T3C2Tx/Bi2Se3 nanoflower-modified screen-printed electrodes for electrochemical detection of trace Pb2+ and Cd2+ in artificial sweat","authors":"Haozi Lu ,&nbsp;Wei Zhou ,&nbsp;Ye Li ,&nbsp;Meiqing Yang ,&nbsp;Jifei Yang ,&nbsp;Can Hu ,&nbsp;Yabo Ogunduyile ,&nbsp;Travis Hu ,&nbsp;Gang Yu ,&nbsp;Huimin Li ,&nbsp;Ming Zhang ,&nbsp;Song Liu","doi":"10.1016/j.bios.2026.118389","DOIUrl":"10.1016/j.bios.2026.118389","url":null,"abstract":"<div><div>In the field of non-invasive detection methods, sweat analysis has been increasingly capturing the interest of researchers due to its potential for clinical and health monitoring applications. While the detection of various substances in sweat has been extensively documented, the detection of heavy metal ions such as cadmium (Cd²⁺) and lead (Pb²⁺) is challenging due to their low concentrations, often falling below 100 ppb. Sweat testing also faces challenges such as variability in sweat production and evaporation rates, biological fouling, and sensor sensitivity decay, which significantly affect the accurate measurement of biomarker levels in sweat. Traditionally, machine learning in the biosensing domain is often employed for final data analysis and fitting, but its application in optimizing experimental conditions is less common. In this study, we developed a sensing platform based on Ti₃C₂T_x/Bi₂Se₃ nanoflower-modified screen-printed carbon electrodes (SPCE) for the detection of Cd²⁺ and Pb²⁺ in simulated sweat. The sensor demonstrates a broad detection range and relatively low limits of detection (LOD), with a linear detection range of 10–150 ppb and an LOD of 2.88 ppb for Cd²⁺, and a linear detection range of 5–150 ppb and an LOD of 3.45 ppb for Pb²⁺. In addition, an LSBoost–NGO machine-learning framework was employed to model the dependence of the stripping current on key experimental variables and to identify operating conditions that maximize analytical performance. These results demonstrate the potential of Ti₃C₂T_x/Bi₂Se₃/SPCEs combined with machine-learning-guided optimization for sweat-based monitoring of trace heavy metal ion.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118389"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145975918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomimetic self-sustained ion oscillation in miscibility gap and chemical inductance impact in potassium sensing","authors":"Marini L ,&nbsp;Roop L. Mahajan ,&nbsp;Chithra Lekha P ,&nbsp;T. Devasena","doi":"10.1016/j.bios.2026.118413","DOIUrl":"10.1016/j.bios.2026.118413","url":null,"abstract":"<div><div>A biomimetic potassium ion-selective electrode (ISE) was developed using a graphene oxide (GO)-coated nickel foam (NF) substrate functionalized with a polyethylene glycol (PEG)-based ion-selective layer (ISL). The sensing mechanism centers on a miscibility gap formed within the ISL due to PEG's amphiphilic nature when complexed with potassium iodide—driving phase separation into hydrophilic and hydrophobic domains. This phase heterogeneity introduces interfacial barriers that delay ionic relaxation and facilitate self-sustained ion oscillation, as evidenced by low-frequency inductive behavior in electrochemical impedance spectroscopy (EIS)—demonstrating a chemical inductor effect. The design eliminates the need for costly ionophores, such as valinomycin, presenting a scalable and cost-effective sensing platform. The sensor exhibited a linear response to K⁺ concentrations ranging from 0.5 to 10 mM, with a high sensitivity of 14.09 × 10² mA/mM·cm² and a detection limit of 0.69 mM. Selectivity tests showed minimal interference from Na⁺, Ca²⁺, urea, NH₄⁺, and ascorbic acid ions, and the sensor demonstrated excellent stability over 100 cycles. These findings provide a robust framework for developing low-cost, biomimetic electrochemical potassium sensing.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118413"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146016861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel ratiometric biosensor based on germanene nanosheets for rapid and sensitive BRCA1 detection in differentiating T cells","authors":"Yingying Huang ,&nbsp;Shu Xiao ,&nbsp;Zhiming Zhong ,&nbsp;Kamning Yuen ,&nbsp;Bohan Yin ,&nbsp;Jiareng Chen ,&nbsp;Jiaxiang Yan ,&nbsp;Jun Peng ,&nbsp;Qin Zhang ,&nbsp;Fengqi Wang ,&nbsp;Bin Liu ,&nbsp;Siu Hong Dexter Wong ,&nbsp;Mo Yang","doi":"10.1016/j.bios.2026.118411","DOIUrl":"10.1016/j.bios.2026.118411","url":null,"abstract":"<div><div>The BRCA1 gene is essential for the developmental regulation and function of T lymphocytes, yet its mRNA expression during T-cell differentiation remains unclear due to the lack of effective in-situ monitoring tools. To address this, we developed a ratiometric fluorescent nanosensor based on Förster resonance energy transfer (FRET) for reliable quantification of BRCA1 mRNA in living CD8⁺ T cells. The sensor comprises a fluorescein-labeled DNA probe (FAM_cDNA) assembled with Triton X-100-modified methylgermanene nanosheets (GeT), forming an efficient FRET pair. In the absence of the target, FAM_cDNA adsorbs onto the GeT surface, resulting in a FRET effect that quenches FAM fluorescence and enhances GeT emission by 1.63-fold. Upon specific hybridization with BRCA1 mRNA, the probe detaches, disrupting the FRET process and causing a quantitative ratiometric shift (I₅₂₀/I₆₄₀). This self-calibrating system demonstrates high sensitivity, with detection limits of 18.1 pM (R² = 0.985) for synthetic DNA and 17.2 pM (R² = 0.996) for mRNA, and a rapid response time (∼10 min). Importantly, the nanoprobe enabled ratiometric imaging of endogenous BRCA1 mRNA in living CD8⁺ T cells, revealing a significant increase in the I₅₂₀/I₆₄₀ ratio during activation, visually confirming BRCA1 upregulation consistent with RNA-seq data. This work provides a robust assay for T-cell studies and highlights red-emissive germanene as a promising platform for ratiometric biosensing.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118411"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146016800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-situ sampling and monitoring ctDNA from dermal interstitial fluid for tumor diagnosis","authors":"Zhenyu Wu ,&nbsp;Jing Chen ,&nbsp;Yuzhe Zhang ,&nbsp;Rufei Ge ,&nbsp;Yan Li ,&nbsp;Khaydar E. Yunusov ,&nbsp;Fozil Mamarayim Ugli Turakulov ,&nbsp;Guohua Jiang","doi":"10.1016/j.bios.2026.118431","DOIUrl":"10.1016/j.bios.2026.118431","url":null,"abstract":"<div><div>Detecting circulating tumor DNA (ctDNA) in skin interstitial fluid (ISF) offers a non-invasive cancer diagnostic route, though clinical translation is often hindered by low extraction efficiency and sensitivity. This study presents an integrated microneedle-electrode platform for <em>in situ</em> sampling and monitoring of ctDNA. Utilizing reverse iontophoresis, ctDNA is extracted from the dermal ISF and subsequently detected via a signal-amplified rGO/PPy/Au NPs electrochemical sensor. The platform facilitates quantitative detection with a broad linear range (10 fM to 1 nM) and a low detection limit of 1.92 fM. It demonstrates high specificity against base mismatches and biological interferents, alongside strong reproducibility. In vivo testing on a murine tumor model showed results significantly correlated with qPCR and ddPCR, highlighting its potential for point-of-care cancer diagnostics and disease monitoring.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118431"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A stretchable continuous glucose monitor for skin-conformable wound management","authors":"Binbin Cui ,&nbsp;Ivo Pang ,&nbsp;Shilei Dai,&nbsp;Xiaonan Chen,&nbsp;Dingyao Liu,&nbsp;Xinyu Tian,&nbsp;Wensheng Lin,&nbsp;Jing Bai,&nbsp;Xinran Zhang,&nbsp;Shiming Zhang","doi":"10.1016/j.bios.2025.118345","DOIUrl":"10.1016/j.bios.2025.118345","url":null,"abstract":"<div><div>Chronic wounds, especially those linked to diabetes, require continuous monitoring and timely interventions to prevent severe complications. The emergence of smart bandages equipped with multifunctional sensors offers a promising avenue to alleviate the burden on healthcare systems while minimizing delays in medical care. However, conventional electrochemical sensors often face limitations, including inadequate detection sensitivity and poor mechanical compatibility with biological tissue, thereby restricting their utility in smart bandages for long-term wound care applications. In this study, we present a stretchable continuous glucose monitor (CGM), based on organic electrochemical transistors (OECTs), for chronic wound care. By leveraging the amplification capability and the mechanical properties of stretchable OECTs, this platform achieves both high sensitivity and tissue compatibility. To demonstrate the potential for practical use, the sensors are integrated into a compact, coin-sized wearable readout system, enabling continuous and comfortable wound monitoring.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118345"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146016872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photoelectrochemical bioimaging of redox events for 3D cultured cells via hematite-based light-addressable electrodes","authors":"Tomoyuki Ogawa ,&nbsp;Ryo Yamatake ,&nbsp;Yusuke Kanno ,&nbsp;Takasi Nisisako ,&nbsp;Yuvaraj M. Hunge ,&nbsp;Kosuke Ino ,&nbsp;Shan Liu ,&nbsp;Chen-Zhong Li ,&nbsp;Tatsuo Yoshinobu ,&nbsp;Hitoshi Shiku ,&nbsp;Hiroya Abe","doi":"10.1016/j.bios.2026.118414","DOIUrl":"10.1016/j.bios.2026.118414","url":null,"abstract":"<div><div>Light-addressable electrochemistry offers a contact-free route to map chemical activity with spatial precision. Bioimaging in three-dimensional (3D) cultures remains challenging under operating conditions dominated by water oxidation. This work establishes hematite (Fe₂O₃) light-addressable electrodes that operate under visible light to image redox events in complex microenvironments. Hydrothermally synthesized hematite films were structurally and photoelectrochemically characterized, revealing a visible absorption edge and favorable band energetics for driving biomolecule oxidation at comparatively low bias. Photoelectrochemical performance was quantified by linear sweep voltammetry, cyclic voltammetry, and chronoamperometry under full-field and focused illumination. Using ferrocenemethanol as a model analyte, bias windows were identified that suppress background oxidation and enable analysis via differential photocurrent. For hydrogen peroxide (H₂O₂), calibration under full-field illumination yielded a limit of detection of 0.74 μM (R² = 0.96); focused-spot operation preserved spatial resolving power with a limit of detection (LOD) of 2.6 μM, sufficient for biological measurements. A glass-masking evaluation quantified spatial resolution with a full width at half maximum of 120 ± 22 μm, and stability tests showed no significant degradation over 100 illumination cycles. The photoelectrochemical imaging resolved diffusion barriers and concentration gradients around MCF-7 spheroids. The system also quantified localized, enzyme-generated H₂O₂ using glucose oxidase beads with catalase controls that confirmed the reaction mechanism. Importantly, phorbol 12-myristate 13-acetate stimulation enabled time-resolved visualization of H₂O₂ release from tumor spheroids at micromolar levels. These results position hematite-based photoelectrochemical imaging as a practical, selective, and noninvasive approach for time-dependent chemical mapping in 3D cell models and related bioanalytical applications.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118414"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"4D-printed ELISA needle panel meter","authors":"Hsiao-Chu Chiu,&nbsp;Cheng-Kuan Su","doi":"10.1016/j.bios.2026.118437","DOIUrl":"10.1016/j.bios.2026.118437","url":null,"abstract":"<div><div>Enzyme-linked immunosorbent assay (ELISA) is commonly used to detect and quantify antibiotics in food samples. To realize on-site chloramphenicol (CAP) ELISA without any chromogenic or fluorogenic analysis, we utilized the digital light processing four-dimensional printing (4DP) technique and 2,2′-(ethyl enedioxy)diethanethiol (EDT)-incorporated resins to fabricate a panel meter featuring a redox-responsive needle. It exhibited [hydrogen peroxide (H₂O₂)]-dependent bending when the oxidation of the thioether groups in the copolymer induced imbalanced swelling between the printed EDT-incorporated and nonresponsive layers. We optimized the coating of anti-CAP antibodies on the panel surfaces to perform competitive ELISA. Based on the competition between CAP and CAP-labeled horseradish peroxidase (CAP-HRP), the bound CAP-HRP eliminated the additionally added H₂O₂, resulting in [CAP]-dependent bending of the needle, with a method detection limit of 2.5 pg mL⁻¹. We validated the reliability and applicability of this analytical method by analyzing salmon, shrimp, white meat, pork, honey, and milk samples with their spike analyses and compared the results with those obtained from conventional ELISA, using 3,3′,5,5′-tetramethylbenzidine as a chromogenic substrate and a microplate reader for detection. Our results demonstrate the capability and applicability of 4DP technologies in fabricating smart redox-responsive sensing devices to advance conventional immunochemical assays for on-site quantitative chemical analyses.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118437"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A DNAzyme-CRISPR cascade strategy for preamplification-free detection of Mycobacterium tuberculosis","authors":"Ying Yu ,&nbsp;Jianhong Zhang ,&nbsp;Guoming Xie ,&nbsp;Yu Lin ,&nbsp;Yue Huang ,&nbsp;Chenxin Rao ,&nbsp;Yulei Hou ,&nbsp;Hui Chen","doi":"10.1016/j.bios.2026.118428","DOIUrl":"10.1016/j.bios.2026.118428","url":null,"abstract":"<div><div>Tuberculosis (TB) remains one of the most fatal infectious diseases worldwide, successful treatment is often limited by insufficient diagnostic capabilities. It creates a pressing need for diagnostic methods that combine high sensitivity, specificity, and operational robustness. In this work, we developed a DNAzyme-CRISPR cascade strategy (Dz-CRISPR) for the specific identification of the <em>IS6110</em> sequence <em>of Mycobacterium tuberculosis</em> (MTB). This system integrated a thermodynamically stabilized hairpin probe, an Arch-shaped signal transduction switch, and an allosterically activated CRISPR-Cas12a cascade. The design enabled direct target recognition and subsequent signal amplification without a preamplification step, offering a simplified workflow with enhanced stability. The assay demonstrated a detection limit of 211.3 fM and exhibited high specificity by accurately discriminating the <em>IS6110</em> from specific DNA sequence of non-tuberculous mycobacteria and other common respiratory pathogens. Validation using clinical bronchoalveolar lavage fluid samples further confirmed the method's reliable performance, reproducibility, and satisfactory recovery rates. Current Dz-CRISPR detection strategy provides a reliable and practical solution for tuberculosis diagnosis with high sensitivity, high specificity, and operational robustness, thus demonstrating potential for practical use in resource-constrained areas.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118428"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}