Biosensors and Bioelectronics最新文献

{"title":"Minimalistic, disposable wireless electrochemical sensors: Integrating self-powered amperometry with direct inductive coupling","authors":"Jihun Rho,&nbsp;Andrew Yang,&nbsp;Joanna Sands,&nbsp;H.-S. Philip Wong,&nbsp;Ada S.Y. Poon","doi":"10.1016/j.bios.2025.117940","DOIUrl":"10.1016/j.bios.2025.117940","url":null,"abstract":"<div><div>Point-of-care (PoC) biomolecular sensing enables rapid diagnosis and prognosis to significantly improve medical accessibility. Wireless electrochemical sensing strategies typically use dedicated RFID or Bluetooth chips for wireless transmission along with a microcontroller and potentiostat to convert chemical signals into electrical outputs. However, integrating chips and multiple active components into a sensor limits its miniaturization, power efficiency, and usage in disposable applications. We therefore propose a minimalistic yet sensitive and efficient sensor design using only one active component: an LED. The LED, paired with a photoresistor, converts currents from analyte concentrations into resistive changes, which are directly transmitted via inductive coupling. This pairing further functions as a buffer, isolating the electrochemical cell from the inductive link for stable wireless transmission. LED and electrochemical cell power is derived from a galvanic cell consisting of a pair of electrodes activated upon contact with bodily fluids. The integrated sensor was characterized with H₂O₂ measurement and incorporated into a diaper to demonstrate usability by measuring uric acid in artificial urine. The proposed approach to sensor design enables battery-free amperometry to be integrated with chip-free wireless data transmission, a promising step towards cost-effective and disposable electrochemical sensing.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 117940"},"PeriodicalIF":10.5,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultra-biocompatible PEDOT:DSS-modified dual-mode bi-directional microelectrode arrays reveal phase-locking dynamics across sleep-wake","authors":"Yu Liu ,&nbsp;Qianli Jia ,&nbsp;Jian Miao ,&nbsp;Longhui Jiang ,&nbsp;Jin Shan ,&nbsp;Yu Wang ,&nbsp;Shiya Lv ,&nbsp;Qi Li ,&nbsp;Yaoyao Liu ,&nbsp;Peiyao Jiao ,&nbsp;Yilin Song ,&nbsp;Jinping Luo ,&nbsp;Xinxia Cai","doi":"10.1016/j.bios.2025.117973","DOIUrl":"10.1016/j.bios.2025.117973","url":null,"abstract":"<div><div>Long-term dynamic monitoring of cellular-level neural activity through implantable electrodes holds significant importance for elucidating sleep-wake regulation mechanisms. Nevertheless, the concerning biocompatibility, transient stability, and limited conductivity of conventional electrodes pose substantial challenges for high-quality signal acquisition. This study proposes a method for rapidly electrodepositing ultra-biocompatible poly(3,4-ethylenedioxythiophene):(dextran sulfate) (PEDOT:DSS) on conductive substrates to fabricate dual-mode bi-directional microelectrode arrays (MEAs). PEDOT:DSS utilizes the anionic polysaccharide dextran sulfate as the counterion template, which substantially enhances the biocompatibility of the PEDOT-based conductive polymer compared to conventional poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) coatings. Furthermore, increased surface roughness endows PEDOT:DSS-modified MEAs with superior conductivity (12.56 kΩ at 1 kHz), 2.89-fold higher charge storage capacity and enhanced electrochemical activity. Cyclic voltammetry aging and 1-h ultrasonic treatment demonstrate exceptional stability. During in vivo recordings over seven days, PEDOT:DSS electrodes exhibited only a 23.89 % noise increase and ∼2.52 % signal-to-noise ratio reduction, versus 74.51 % and 29.51 % for PEDOT:PSS. More importantly, multiple sleep-wake correlated neurons are identified showing stage-dependent firing rate variations, providing electrophysiological evidence for neuronal heterogeneity within these nuclei. Beyond firing rates, sleep-stage-specific delta-phase preference is unveiled for the first time. Certain neurons exhibit phase-locking only during wake, suggesting potential regulatory roles through location-specific firing patterns. This work establishes a high-performance electrode-neural interface, deciphers distinct neural activity patterns across sleep-wake stages, and substantially advances the development of implantable electrodes for neuroscientific research and clinical applications.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 117973"},"PeriodicalIF":10.5,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A self-cycling and self-verifying electrochemical and colorimetric dual-modal biosensor for oral squamous cell carcinoma (OSCC) saliva detection","authors":"Jing Li ,&nbsp;Qi Jiang ,&nbsp;Dengxue Qiu ,&nbsp;Ruirui Chang ,&nbsp;Jin Huang ,&nbsp;Qin Xu ,&nbsp;Kemin Wang","doi":"10.1016/j.bios.2025.118000","DOIUrl":"10.1016/j.bios.2025.118000","url":null,"abstract":"<div><div>The early and precise detection of oral cancer overexpression 1 (ORAOV 1) holds significant promise for improving the five-year survival rate of patients with oral squamous cell carcinoma (OSCC). Herein, we propose a self-cycling and self-verifying electrochemical/colorimetric dual-modal biosensor for ORAOV 1 detection in saliva. This cutting-edge biosensor combines a fuel-powered DNA nanomachine with gold-platinum nanoparticles (AuPt) modified zirconium-based metal-organic framework (AuPt@UiO-66) composites. The presence of ORAOV 1 induces the autonomous movement of DNA nanomachines with the help of AuPt-modified fuel DNA. This results in the production of significant quantities of AuPt@UiO-66 precipitates and signal strands (S). By leveraging both electrocatalytic activity and peroxide-like catalytic properties of AuPt@UiO-66, electrochemical/colorimetric dual-channel signals can be generated. The experimental results demonstrated that the electrochemical mode is highly sensitive to ORAOV 1, with a detection limit of 9.16 aM. Although colorimetric mode has moderate sensitivity, it enables signals to be visualized with the naked eye. These two modes can validate each other, improving the accuracy of detection. More importantly, it can effectively discriminate between cancer patients and healthy individuals with good accuracy (AUC = 1), providing new insights into the early and precise diagnosis of OSCC.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 118000"},"PeriodicalIF":10.5,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145084691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flower-like ytterbium Prussian blue analogue nanozyme with freeze-drying method for colorimetric/photothermal/smartphone triple-mode detection of thiosulfate","authors":"Wenting Wang ,&nbsp;Yupeng Ren ,&nbsp;Biao Guan ,&nbsp;Hong Cao ,&nbsp;Wenling Hu ,&nbsp;Lei Li ,&nbsp;Longhua Guo ,&nbsp;Yanbo Zeng","doi":"10.1016/j.bios.2025.117997","DOIUrl":"10.1016/j.bios.2025.117997","url":null,"abstract":"<div><div>The morphology affects the catalytic activity of nanozymes. This work prepared different ytterbium Prussian blue analogue (PBA) nanozymes with flower-like and rod-like morphologies through changing various amount of K₃[Fe(CN)₆] and Yb(NO₃)₃. Flower-like ytterbium PBA (F-YbFe-PBA) prepared using freeze-drying method and lanthanide-doped strategy with 1.5 mmol K₃[Fe(CN)₆] and Yb(NO₃)₃ had highest peroxidase-like (POD-like) activity among other F-YbFe-PBA nanozymes with freeze-drying method and rod-like YbFe-PBA (R-YbFe-PBA) nanozymes with traditional coprecipitation approach. F-YbFe-PBA exhibited higher POD-like activity than R-YbFe-PBA, which was attributed to the higher surface area and larger pore size of F-YbFe-PBA. F-YbFe-PBA demonstrated superior POD-like performance compared to other PBA nanozymes of SmFe-PBA, PrFe-PBA, MnFe-PBA, and CuFe-PBA. F-YbFe-PBA provided excellent POD-like activity with Michaelis constant of 0.16 mM and V_max value of 6.21 × 10⁻⁸ M s⁻¹ for substrate of 3,3′,5,5′-tetramethylbenzidine (TMB). In the presence of H₂O₂, F-YbFe-PBA catalyzes the decomposition of H₂O₂ to generate reactive hydroxyl radicals. However, thiosulfate (S₂O₃²⁻) has the reducibility capability, effectively suppressing the colorimetric reaction. Therefore, F-YbFe-PBA was successfully utilized to construct a triple-mode selective detection combining colorimetric, photothermal, and smartphone sensing for S₂O₃²⁻ analysis. This triple-mode sensor demonstrated excellent linear detection of S₂O₃²⁻ across a range of 0.1–75 μM through colorimetric, photothermal, and smartphone assays, achieving detection limits of 0.01, 0.01, and 0.03 μM, respectively. Notably, a smartphone-based paper senor with red/blue (R/B) values was developed for real-time and portable detection assay of S₂O₃²⁻. The proposed sensor had high selectivity for S₂O₃²⁻. The F-YbFe-PBA-based sensor was successfully employed for S₂O₃²⁻ detection in tap water and milk samples.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 117997"},"PeriodicalIF":10.5,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential-pulse-assisted co-immobilization of multiple aptamers on microelectrode arrays for multiplexed neurotransmitter detection","authors":"Ziheng Hu ,&nbsp;Ruifeng Zhu ,&nbsp;Gabriela Figueroa-Miranda ,&nbsp;Lingyan Feng ,&nbsp;Andreas Offenhäusser ,&nbsp;Dirk Mayer","doi":"10.1016/j.bios.2025.117992","DOIUrl":"10.1016/j.bios.2025.117992","url":null,"abstract":"<div><div>Accurate and simultaneous determination of multiple neurotransmitters (NTs) is crucial for a thorough understanding of brain functions and for diagnosing neurological disorders. We have developed an electrochemical biosensor utilizing aptamer recognition for the highly sensitive and simultaneous detection of multiple neurotransmitters, including glutamate quantification for the first time in multiplex detection. Microelectrode arrays (MEAs) served as biosensing platforms to facilitate the recording of signals from multiple channels, rapid mass transfer rates, and high spatial resolution, fundamental for studying NTs release in nervous tissue. To enhance aptamer receptor loading, gold nanostructures were electrodeposited onto the microelectrodes, enhancing the active surface area and electrode morphology. A potential-pulse-assisted method was employed to achieve site-selective immobilization of three different aptamers on a single MEA chip within 30 min, enabling fast and reproducible sensor fabrication. This unique strategy ensured aptamer-specific immobilization, minimized cross-talk, and allowed for multiplex detection of serotonin, glutamate, and dopamine with high sensitivity and specificity. Additionally, using polyethylene glycol (PEG) as a blocking molecule, the aptamer-functionalized MEAs showed enhanced antifouling properties and maintained detection capabilities in complex environments. This multiplexed detection strategy enables a high-performance and robust biosensor platform with strong clinical relevance, offering substantial potential for <em>in vivo</em> monitoring of neurotransmitter release and the diagnosis of neurological disorders.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 117992"},"PeriodicalIF":10.5,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single–cell Raman imaging reveals fructose impairs brown adipocyte differentiation","authors":"Anoushka Gupta ,&nbsp;Pooja Anantha ,&nbsp;Hidenori Koresawa ,&nbsp;Heqi Xi ,&nbsp;Yu-Hua (Beckham) Yang ,&nbsp;Michael Ka Ho Lee ,&nbsp;Katsumasa Fujita ,&nbsp;Sui-Seng Tee ,&nbsp;Ishan Barman","doi":"10.1016/j.bios.2025.117994","DOIUrl":"10.1016/j.bios.2025.117994","url":null,"abstract":"<div><div>Brown adipose tissue (BAT) plays a pivotal role in energy expenditure and metabolic health, yet the influence of specific sugars like fructose on brown adipocyte development remains poorly understood. Here, we employ high-speed line-illumination Raman imaging, combined with machine learning, to investigate how varying sugar environments – fructose, glucose, or both – impact the differentiation of human brown preadipocytes (HBPs). This label-free, non-destructive method provides spatially-resolved insight into lipid content, composition, and subcellular distribution during adipogenesis. Quantitative ratiometric analysis of Raman spectra reveals that fructose exposure leads to higher lipid unsaturation and esterification, indicative of impaired differentiation. Trajectory inference and unsupervised clustering further identify distinct subpopulations of adipocytes, demonstrating that fructose-treated cells exhibit phenotypes associated with early differentiation stages. Together, our findings reveal a negative regulatory role of fructose in brown adipocyte maturation and highlight Raman imaging as a powerful tool for dissecting metabolic cell states under variable nutrient conditions.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 117994"},"PeriodicalIF":10.5,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145060946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic forecasting of beef freshness using multi-step time series analysis of electronic nose signals","authors":"Xinxing Li ,&nbsp;Runqing Chen ,&nbsp;Hao Zhang ,&nbsp;Jing Chen ,&nbsp;Buwen Liang","doi":"10.1016/j.bios.2025.117977","DOIUrl":"10.1016/j.bios.2025.117977","url":null,"abstract":"<div><div>The preservation of microbial quality in meat products represents a fundamental challenge in contemporary food supply chain management due to the highly perishable nature of these commodities. Although modern testing techniques, particularly electronic nose (E-nose), have shown considerable promise in real-time assessment of freshness status, most applications remain limited to static evaluation rather than dynamic forecasting of future quality trajectories, constraining proactive decision-making processes. To overcome this diagnostic-predictive gap, we propose a framework that integrates E-nose sensing with multi-step time-series forecasting, thereby transforming meat quality monitoring from real-time diagnosis to predictive modeling. In particular, we design an enhanced dual stage attention-based recurrent neural network tailored to microbial growth dynamics and the specific characteristics of E-nose signals, such as limited sample sizes, non-stationary temporal patterns, and gradual signal evolution. Furthermore, the proposed model is further validated on twelve beef regions to ensure robust generalization across heterogeneous tissue-specific spoilage patterns. The experimental results demonstrate that the model is capable of multi-step Total Viable Count (TVC) forecasting across horizons from 1 to 9 h. For 1-h short-term prediction, the model can achieve a mean R² of 0.950 with an RMSE of 0.097, while for long-term forecasting (9 h), it still maintained an R² above 0.859 across 12 tissues, demonstrating both superior predictive accuracy and sustained temporal stability. In summary, this work establishes a time-series forecasting framework that leverages sensor-derived signal trajectories to capture microbial growth dynamics and the evolution of TVC within beef. By advancing freshness evaluation from static detection to predictive modeling with hour-level resolution, the approach enables reliable estimation of remaining shelf life and provides a quantitative paradigm for meat quality management.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 117977"},"PeriodicalIF":10.5,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145096318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smart exercise device using triboelectric self-powered sensor for high intensity interval training (HIIT)","authors":"Divij Bhatia,&nbsp;Dongchan Kim,&nbsp;Hyung-Soon Park","doi":"10.1016/j.bios.2025.117995","DOIUrl":"10.1016/j.bios.2025.117995","url":null,"abstract":"<div><div>High intensity interval training (HIIT) where periods of high intensity exercise are interspersed with periods of low intensity exercise or rest, packs a high-power workout into a short period resulting in higher oxygen uptake compared to other training methods. Commercial equipment used for such exercise protocols either requires manual adjustment or provides pre-programmed options without adapting to the user's performance. It would be much more convenient if the exercise equipment could detect how well the user is exercising and automatically adjust the training intensity level. Thus, in this work, we developed a smart exercise device using a triboelectric nanogenerator (Exercise-TENG). The device utilized the input torque increasing characteristic of the planetary gear system. As the subject operated the Exercise-TENG, energy harvested by the TENG was stored into a capacitor. The level of energy harvested into the capacitor was both an indicator of the user's exercise performance, and a trigger to increase or decrease the input torque required to operate the device. Furthermore, the user could be motivated to exercise by providing real-time feedback on the amount of energy harvested via a LabVIEW interface. Finally, we conducted HIIT bicep curl and triceps extension exercises using the Exercise-TENG and measured muscle activation levels to verify its effectiveness. Compared to other exercise devices, Exercise-TENG is a personalized solution that automatically adapts to the users' abilities with a unique motivation element especially needed for patient rehabilitation. We expect that our device will be a viable product for home exercise or patient rehabilitation applications.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 117995"},"PeriodicalIF":10.5,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145096315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A DNA-programmed magnetic photoactive probe enabling discriminative photoelectrochemical sensing of diverse targets","authors":"Jiaxin Guo ,&nbsp;Ying Li ,&nbsp;Gaiping Li ,&nbsp;Shusheng Zhang ,&nbsp;Baoxian Ye ,&nbsp;Lina Zou","doi":"10.1016/j.bios.2025.117993","DOIUrl":"10.1016/j.bios.2025.117993","url":null,"abstract":"<div><div>The integration of magnetic nanomaterials and photoelectrochemical aptamer sensing strategies offers significant potential for rapid separation of target molecules, improved detection sensitivity and enhanced accuracy. However, the limited photoelectrochemical activity of magnetic nanomaterials and the high cost of aptamer sensors hinder their advancement. This study innovatively realized the independent, sensitive, and precise photoelectrochemical detection of different target molecules on same platform by constructing ultra-efficient magnetic photoactive probe, ZnFe₂O₄/Bi₂O₂S/AuNPs/C1-C2. The designed ZnFe₂O₄/Bi₂O₂S/AuNPs exhibited high photocurrent response activity, efficient magnetic separation performance, and high aptamer immobilization capacity. Additionally, the elaborately designed DNA duplex C1-C2 probe also served dual functions, acting as “aptamer” for target identification and “bridging linker” to connect ZBA or capture hemin for different target detection, thereby inducing photocurrent polarity reversal or photocurrent enhancement. These two targets share the core recognition component, the same electrode interface, and testing conditions, yet generate photocurrent signals with opposite polarity, enabling the determination of various targets on the same sensing platform and significantly improving sensing efficiency. The sensor achieved a low detection limit for chloramphenicol (24.6 pM) and ampicillin (14.5 fM), and demonstrated high precision with relative standard deviations of 4.5 % for chloramphenicol detection and 2.0 % for ampicillin detection. Furthermore, this strategy maximizes the advantages of DNA and nanomaterials, simplifies operation procedures and opens new avenues for developing low-cost, highly reliable multi-target detection technologies.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 117993"},"PeriodicalIF":10.5,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly sensitive printed pH sensor using CB/PANI nanocomposite for POC diagnosis of orthopedic infections","authors":"Christian Gosti ,&nbsp;Luca Fiore ,&nbsp;Fabio Domenici ,&nbsp;Gennaro Gentile ,&nbsp;Silvano Del Gobbo ,&nbsp;Raffaele Vitiello ,&nbsp;Giulio Maccauro ,&nbsp;Fabiana Arduini","doi":"10.1016/j.bios.2025.117991","DOIUrl":"10.1016/j.bios.2025.117991","url":null,"abstract":"<div><div>Herein, we reported a potentiometric pH screen-printed electrode incorporating Carbon Black (CB) nanomaterial and chemically stable pH-sensitive Polyaniline (PANI) for revealing orthopedic infections, as pH is a pivotal biomarker. A dispersion of CB/PANI nanocomposite was easily drop-cast onto the working electrode surface to deliver a straightforward and efficient functionalization of the printed sensor. An in-depth psychochemical and morphological characterization of the nanocomposite was conducted by Dynamic Light Scattering, Electrophoretic Light Scattering, and Transmission Electron Microscopy. 90 % CB – 10 % PANI mixture was selected for electrode functionalization owing to an outstanding dispersibility and an efficient trade-off between linearity in the pH range 3–8 (R² = 0.994), reliable reproducibility (RSD% = 0.9 %, n = 3), and lack of memory effect, alongside noteworthy sensitivity (−74 ± 3 mV/pH unit). The proposed sensor further showcased a notable selectivity, resulting in negligible pH deviations when exposed to feasible interfering candidates in synovial fluid. Storage stability was also tracked for over 1 month by monitoring the potentiometric response of the designed sensor, kept dry at room temperature. The ANOVA analysis and post-hoc Tukey test demonstrated a stable potential signal, which has been achieved from the second week, with a performance coherence up to one month. Finally, sensor validation was carried out in real samples of healthy and infected synovial fluid by comparing the response of the developed sensor to the pH-meter and the outcome of microbiological analysis, demonstrating the accuracy and the effectiveness of the stable, highly sensitive, and mass-produced printed sensor.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 117991"},"PeriodicalIF":10.5,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145096317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}