Biosensors and Bioelectronics最新文献_第9页

Troponin I biomarker sensing from clinical patient samples using molecularly imprinted polymer nanoparticles for advancing healthcare approaches in cardiovascular disease 利用分子印迹聚合物纳米颗粒对临床患者样本中的肌钙蛋白I生物标志物进行传感，以推进心血管疾病的医疗保健方法

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-04-10 DOI: 10.1016/j.bios.2025.117467

Joshua Saczek , Oliver Jamieson , Jake McClements , Amy Dann , Rhiannon E. Johnson , Alexander D. Stokes , Robert D. Crapnell , Craig E. Banks , Francesco Canfarotta , Ioakim Spyridopoulos , Alan Thomson , Azfar Zaman , Katarina Novakovic , Marloes Peeters

{"title":"Troponin I biomarker sensing from clinical patient samples using molecularly imprinted polymer nanoparticles for advancing healthcare approaches in cardiovascular disease","authors":"Joshua Saczek , Oliver Jamieson , Jake McClements , Amy Dann , Rhiannon E. Johnson , Alexander D. Stokes , Robert D. Crapnell , Craig E. Banks , Francesco Canfarotta , Ioakim Spyridopoulos , Alan Thomson , Azfar Zaman , Katarina Novakovic , Marloes Peeters","doi":"10.1016/j.bios.2025.117467","DOIUrl":"10.1016/j.bios.2025.117467","url":null,"abstract":"<div><div>Cardiac troponin I (cTnI) is a critical protein biomarker for heart attack diagnosis. This study presents a thorough analysis of a novel biosensing device utilizing molecularly imprinted polymer nanoparticles (nanoMIPs) for detecting cTnI in clinical patient serum samples post myocardial infarction. The methodology, based on the heat-transfer method approach, offers faster measurements times than the current gold standard and sample volumes equivalent to a single blood drop. Biomarker binding shows performance comparable to a high-sensitivity ELISA, accurately identifying patients with elevated cTnI levels (R<sup>2</sup> = 0.893). The cTnI peak concentration time variations are attributed to heterogeneous serum complexes, with different troponin complex sizes potentially generating differing thermal insulation levels. Comparison with an established patient database demonstrates robust correlations between our cTnI concentrations and clinical parameters (R<sup>2</sup> = 0.855). This underscores the potential of nanoMIP sensors for sensitive cTnI detection, providing insights into post-heart attack biomarker levels. Furthermore, our methodology presents the additional benefits of being low cost and portable enabling measurements at time and place of patients. Consequently, it holds the potential to become a vital part of the diagnostic pathway for heart attack treatment, ultimately reducing healthcare costs and improving patient outcomes.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"282 ","pages":"Article 117467"},"PeriodicalIF":10.7,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyphenol briged bimetallic-heterostructure inducing chiral peroxidase nanozyme activity for enantiomers identification in gastric cancer 诱导手性过氧化物酶纳米酶活性的多酚双金属对映体结构，用于胃癌对映体的鉴定

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-04-09 DOI: 10.1016/j.bios.2025.117449

Qiu-hong Yao , Mei-huang Zeng , Chen Zhang , Feng Zheng , Jing-wen Jin , Ting-xiu Ye , Yi-ting Wang , Xiao-mei Chen , Zhi-yong Guo , Xi Chen

{"title":"Polyphenol briged bimetallic-heterostructure inducing chiral peroxidase nanozyme activity for enantiomers identification in gastric cancer","authors":"Qiu-hong Yao , Mei-huang Zeng , Chen Zhang , Feng Zheng , Jing-wen Jin , Ting-xiu Ye , Yi-ting Wang , Xiao-mei Chen , Zhi-yong Guo , Xi Chen","doi":"10.1016/j.bios.2025.117449","DOIUrl":"10.1016/j.bios.2025.117449","url":null,"abstract":"<div><div><sub>D</sub>-alanine (<sub>D</sub>-Ala) and <sub>D</sub>-proline(<sub>D</sub>-Pro) levels dramatically increase in the bio-fluids of gastric cancer (GC) patients, which are suggested to be potential biomarkers associated with GC. Therefore, monitoring the concentrations of <sub>D</sub>-Pro and <sub>D</sub>-Ala in the body fluids of GC patients is of great significance. In this study, a highly sensitive Surface Enhanced Raman Scattering (SERS) sensor has constructed based on bimetallic heterostructure nanoenzyme (3-MPBA: Co-TA@Ag NPs) for <sub>D</sub>-AAs detection in GC bio-fluids. Attributing to the formation of Ag-Co bimetallic dual active centers, the electron transfer behaviors accelerated boron hydroxyl group ionization in neutral pH, as well as the ultra-robust hydroxyl alcohol esterification reaction occurred between <sub>D</sub>-Ala and <sub>D</sub>-Pro and 3-MPBA. By simply testing different absorbance intensities at various concentrations and sample, a sensor for noninvasive detection of <sub>D</sub>-Pro/Ala is constructed with good specificity and efficiency. Besides, the quantitative determination of <sub>D</sub>-Pro/Ala (limits of detection (LOD) 2.37 μM and 2.15 μM for <sub>D</sub>-Pro/Ala. Furthermore, based on the differential level of <sub>D</sub>-Pro/Ala in GC bio-fluids, the differentiation of saliva samples with <sub>D</sub>-Pro/Ala can also be implemented by the proposed method, showing great potential in GC diagnosis.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"282 ","pages":"Article 117449"},"PeriodicalIF":10.7,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physically grounded deep learning-enabled gold nanoparticle localization and quantification in photonic resonator absorption microscopy for digital resolution molecular diagnostics 物理接地深度学习使金纳米粒子定位和定量在光子谐振腔吸收显微镜用于数字分辨率分子诊断

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-04-09 DOI: 10.1016/j.bios.2025.117455

Hankeun Lee , Siyan Li , Leyang Liu , Weijing Wang , Takhmina Ayupova , Joseph Tibbs , Chansong Kim , Ying Fang , Minh N. Do , Brian T. Cunningham

{"title":"Physically grounded deep learning-enabled gold nanoparticle localization and quantification in photonic resonator absorption microscopy for digital resolution molecular diagnostics","authors":"Hankeun Lee , Siyan Li , Leyang Liu , Weijing Wang , Takhmina Ayupova , Joseph Tibbs , Chansong Kim , Ying Fang , Minh N. Do , Brian T. Cunningham","doi":"10.1016/j.bios.2025.117455","DOIUrl":"10.1016/j.bios.2025.117455","url":null,"abstract":"<div><div>Accurate molecular biomarker detection with digital-resolution sensitivity is essential for applications such as disease diagnostics, therapeutic studies, and biomedical research. Here, we present LOCA-PRAM (LOcalization with Context Awareness), a deep learning-based method integrated with a Photonic Resonator Absorption Microscope (PRAM) to achieve digital-resolution detection of biomolecules using gold nanoparticles (AuNPs) as molecular tags. LOCA-PRAM leverages photonic crystal (PC)-AuNP resonant-coupling to enhance signal contrast, facilitating precise quantification of target molecules without partitioning the sample into droplets or enzymatic amplification. Through registration of PRAM images with Scanning Electron Microscopy (SEM) images, we empirically obtain the point spread function (PSF) of AuNP tags, enabling realistic training data generation for the deep learning framework. LOCA-PRAM surpasses conventional image processing method in accuracy and sensitivity, achieving reliable AuNP detection and localization even in high-density conditions, minimizing false-positive and false-negative quantifications and expending the dynamic range of assay. Benchmarking with SEM-derived ground truth confirms LOCA-PRAM's sub-pixel resolution and ability to accurately quantify AuNPs with overlapping PSF. Overall, the PRAM combined with LOCA-based AuNP digital counting enables real-time, high-precision detection of molecular biomarkers, advancing digital-resolution biosensing for biomedical research and diagnostics.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"281 ","pages":"Article 117455"},"PeriodicalIF":10.7,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and ultrasensitive quantification of H. pylori infections on gastric and stool human samples with a photonic label-free nanobiosensor 用光子无标记纳米生物传感器鉴定和超灵敏定量人胃和粪便中幽门螺杆菌感染

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-04-09 DOI: 10.1016/j.bios.2025.117459

Alejandro Astúa , M.-Carmen Estevez , M. José Ramírez-Lázaro , Xavier Calvet , Sergio Lario , Laura M. Lechuga

{"title":"Identification and ultrasensitive quantification of H. pylori infections on gastric and stool human samples with a photonic label-free nanobiosensor","authors":"Alejandro Astúa , M.-Carmen Estevez , M. José Ramírez-Lázaro , Xavier Calvet , Sergio Lario , Laura M. Lechuga","doi":"10.1016/j.bios.2025.117459","DOIUrl":"10.1016/j.bios.2025.117459","url":null,"abstract":"<div><div><em>Helicobacter pylori</em> is a widespread bacterium that infects the stomach, causing gastric disorders associated with high morbidity and mortality worldwide. Current methods for identifying and quantifying this pathogen rely on invasive and non-invasive tests. Although combining these methods allows accurate diagnosis, they have multiple drawbacks, and there is no single reliable gold standard test. New, more sensitive strategies involving molecular techniques, such as digital PCR, have been developed but require complex and expensive instruments. Herein, we implement and validat a nanophotonic bimodal waveguide (BiMW) biosensor for the sensitive and accurate detection of <em>H. pylori</em> in gastric biopsies and stool. This biosensor offers real-time, label-free detection, high sensitivity, and the capability to be integrated into compact devices. By employing monoclonal antibodies targeting specific membrane proteins found in <em>H. pylori</em>, the biosensor enables unique recognition of the bacterium, demonstrating its potential as an alternative diagnostic tool. The BiMW biosensor provides highly accurate <em>H. pylori</em> quantification in under 20 min, with limits of detection (LOD) of 89 ± 35 CFU/mL for antrum gastric biopsies and 82 ± 9 CFU/mL for stool samples. Clinical validation with 40 samples (20 gastric biopsies and 20 stool samples) showed sensitivity and specificity of 90 % for gastric biopsies and 95 % for stool samples, offering diagnostic reliability equivalent to semiquantitative ELISA tests and enabling more efficient and timely detection of <em>H. pylori</em> infections. This test can significantly improve the speed of diagnosis and contribute to the development of more effective strategies for <em>H. pylori</em> eradication.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"281 ","pages":"Article 117459"},"PeriodicalIF":10.7,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocascade-inspired amplified oxygen vacancy effect on facet-engineered BiOI for ultrasensitive photoelectrochemical detection of 8-oxoguanine DNA glycosylase 生物级联激发的放大氧空位效应在面工程BiOI上用于超灵敏光电化学检测8-氧鸟嘌呤DNA糖基化酶

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-04-09 DOI: 10.1016/j.bios.2025.117466

Mengmeng Gu, Xia Yu, Qing Zhou, Xiuming Wu, Jing Wang, Guang-Li Wang

{"title":"Biocascade-inspired amplified oxygen vacancy effect on facet-engineered BiOI for ultrasensitive photoelectrochemical detection of 8-oxoguanine DNA glycosylase","authors":"Mengmeng Gu, Xia Yu, Qing Zhou, Xiuming Wu, Jing Wang, Guang-Li Wang","doi":"10.1016/j.bios.2025.117466","DOIUrl":"10.1016/j.bios.2025.117466","url":null,"abstract":"<div><div>Sensitive detection of 8-oxoguanine DNA glycosylase 1 (hOGG1) activity is essential for early cancer screening and therapy, yet the potential of photoelectrochemistry (PEC) for hOGG1 detection is untapped. Herein, we explore a new bioreaction for sensitive PEC detection of hOGG1 through biocascade reaction provoked amplified oxygen vacancy (O<sub>V</sub>) effect on facet-engineered BiOI. Specifically, the recognition of hOGG1 activated the catalytic peptide hydrolysis reaction of thrombin (Thr), producing the O<sub>V</sub> stimulator <em>p</em>-aminophenol (AP). AP was recycled via the diphosphatase (DI, EC 1.6.99.2) mediated reaction, inducing the formation of abundant surface O<sub>V</sub> on BiOI with exposed (110) facet (BI-110). This process strikingly enhanced the carrier separation efficiency and augmented the photocurrent gain, enabling highly sensitive detection of hOGG1 with a linear range of 1.0 × 10<sup>−4</sup> to 80 U/mL and a low detection limit of 2.0 × 10<sup>−5</sup> U/mL. This study addresses the challenge of developing effective PEC assays for hOGG1 by elucidating a new principle of the biocascade reaction-sparked O<sub>V</sub> effect with facet selectivity, thus filling a gap in PEC detection method for this enzyme.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"281 ","pages":"Article 117466"},"PeriodicalIF":10.7,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MXene-enabled organic synaptic fiber for ultralow-power and biochemical-mediated neuromorphic transistor 用于超低功耗生化介导神经形态晶体管的mxene驱动有机突触纤维

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-04-09 DOI: 10.1016/j.bios.2025.117443

Xing Qing , Qing Xiao , Dong Wang , Guoliang Yang , Bin Chen , Caoyang Zhang , Mufang Li , Dan Liu , Weiwei Lei

{"title":"MXene-enabled organic synaptic fiber for ultralow-power and biochemical-mediated neuromorphic transistor","authors":"Xing Qing , Qing Xiao , Dong Wang , Guoliang Yang , Bin Chen , Caoyang Zhang , Mufang Li , Dan Liu , Weiwei Lei","doi":"10.1016/j.bios.2025.117443","DOIUrl":"10.1016/j.bios.2025.117443","url":null,"abstract":"<div><div>Fibrous bioelectronic provides an intrinsically accessible platform for artificial nerve and real-time physiological perception. However, advanced fiber-based artificial synapse remains a challenge due to the contradictory conductance demands for brain-like energy consumption and ultrasensitive biomarker perception. Herein, taking advantage of the highly accessible surface, rich functional groups and excellent electrical conductivity, a hierarchical nanostructured MXene (Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub>)-enabled artificial neurofiber was proposed for neuromorphic organic electrochemical transistors (OECT) with biomolecule-mediated plasticity. The device can successfully emulate the typical short-term/long-term synaptic behaviors in both protonic gel electrolyte and aprotic ionic liquid gel electrolyte, with a minimum energy consumption of 1.21 fJ/spike and 0.10 fJ/spike. Uric acid (UA), a neurocognitive function and acute joint pain involved biomarker, and its specific enzyme were investigated to simulate the neurotransmitter-receptor induced postsynaptic synaptic weight modulation and pain sensitization process. The OECT showed excellent sensitivity and anti-interference performance. Moreover, selective and concentration-depended synaptic behaviors were successfully achieved in both phosphate-buffered saline (PBS) and artificial urine environments with significant memory effects. This study provided a potential to combine artificial neuromorphic devices with biological sensory neural networks.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"281 ","pages":"Article 117443"},"PeriodicalIF":10.7,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aptamer selection of radiation-sensitive protein p21 and electrical impedance detection-based applications in radiation dose assessment 辐射敏感蛋白p21适配体选择及电阻抗检测在辐射剂量评估中的应用

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-04-09 DOI: 10.1016/j.bios.2025.117447

Chenjun Bai , Tinghui Zhang , Tao Wu , Yuting Wang , Shibo Yao , Cui Wang , Jinpeng Tan , Ruixue Huang , Pingkun Zhou

{"title":"Aptamer selection of radiation-sensitive protein p21 and electrical impedance detection-based applications in radiation dose assessment","authors":"Chenjun Bai , Tinghui Zhang , Tao Wu , Yuting Wang , Shibo Yao , Cui Wang , Jinpeng Tan , Ruixue Huang , Pingkun Zhou","doi":"10.1016/j.bios.2025.117447","DOIUrl":"10.1016/j.bios.2025.117447","url":null,"abstract":"<div><div>Radiation dose assessment is the main basis for the diagnosis of acute radiation sickness. At present, there is a lack of rapid and portable dose assessment methods, which has an important impact on the rapid diagnosis and precise treatment of radiation accident patients and nuclear practitioners. We selected and obtained specific aptamers for radiation-sensitive protein p21 protein by the magnetic cross-linking precipitation (MCP)-SELEX procedure. The aptamer has a high affinity for binding to the p21 protein and its K<sub>d</sub> value is 2.21 × 10<sup>−7</sup> mol/L. We subsequently established a new method for radiation dose assessment of an electrochemical impedance (EIS) aptasensor with screen-printed electrode chips. There was a good dose-effect relationship between the p21 protein expression level in PBMCs in human peripheral blood detected by this method within the dose range of 0–10 Gy, and detection limit of radiation dose is 0.38 Gy (LOD, S/N = 3). This dose range covers the diagnostic range of acute radiation sickness in the bone marrow. This method is not only portable but also fast, saving hours to days compared with the previous dose assessment method based on radiation sensitive protein. It can be applied to the rapid and portable diagnosis of acute radiation sickness.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"282 ","pages":"Article 117447"},"PeriodicalIF":10.7,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Palm-sized CRISPR sensing platform for on-site Mycoplasma pneumoniae detection 手掌大小的CRISPR传感平台用于现场肺炎支原体检测

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-04-09 DOI: 10.1016/j.bios.2025.117458

Jiajun Li , Haiqian Huang , Zerui Song , Shiying Chen , Jingsong Xu , Jun Yang , Chenyue Zheng , Yu Liu , Junheng Zhang , Li Cao , Qian Liu , Qiong Li , Min Li , Zhen Gu , Hua Wang

{"title":"Palm-sized CRISPR sensing platform for on-site Mycoplasma pneumoniae detection","authors":"Jiajun Li , Haiqian Huang , Zerui Song , Shiying Chen , Jingsong Xu , Jun Yang , Chenyue Zheng , Yu Liu , Junheng Zhang , Li Cao , Qian Liu , Qiong Li , Min Li , Zhen Gu , Hua Wang","doi":"10.1016/j.bios.2025.117458","DOIUrl":"10.1016/j.bios.2025.117458","url":null,"abstract":"<div><div>Pneumonia remains a prevalent childhood illness and is the foremost cause of mortality due to infectious diseases among children under the age of five. <em>Mycoplasma pneumoniae</em> (<em>M. pneumoniae</em>) causes the most frequent type of atypical pneumonia in this age group, has raised global health concern. Therefore, there is a pressing need for a rapid, low cost, and user-friendly method for the early diagnosis of <em>M. pneumoniae</em> pneumonia. Herein, we develop a CRISPR sensing platform for on-site <em>M. pneumoniae</em> detection, termed CRAFT (<strong>C</strong>rispr-based rapid assay device for field testing). The CRAFT provides “sample in-result out” functionality. It completed sample processing and nucleic acid extraction within 5 min at room temperature, with efficiency comparable to commercial kits. RPA and CRISPR/Cas12a reagents were isolated in a closed tube using a movable magnetic bead valve, and the RPA product was then mixed with the CRISPR reagent. The limit of detection for <em>M. pneumoniae</em> using CRAFT was 100 copies/μL, and the method exhibited no cross-reactivity with other respiratory pathogens. CRAFT was utilized to validate 50 clinical samples, and the results demonstrated 100 % consistency with those obtained by qPCR. This versatile platform holds significant potential for point-of-care testing of <em>M. pneumoniae</em>, particularly in resource-limited settings.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"281 ","pages":"Article 117458"},"PeriodicalIF":10.7,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Handheld ISFET Lab-on-Chip detection of YAP1 nucleic acid and AR-FL and AR-V7 mRNA from liquid biopsies for prostate cancer prognosis 手持式ISFET芯片实验室检测前列腺癌液体活检中YAP1核酸及AR-FL和AR-V7 mRNA的预后

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-04-08 DOI: 10.1016/j.bios.2025.117407

Joseph Broomfield , Melpomeni Kalofonou , Costanza Gulli , Sue M. Powell , Rayzel C. Fernandes , Damien A. Leach , Nicolas Moser , Naveed Sarwar , Stephen Mangar , Charlotte L. Bevan , Pantelis Georgiou

{"title":"Handheld ISFET Lab-on-Chip detection of YAP1 nucleic acid and AR-FL and AR-V7 mRNA from liquid biopsies for prostate cancer prognosis","authors":"Joseph Broomfield , Melpomeni Kalofonou , Costanza Gulli , Sue M. Powell , Rayzel C. Fernandes , Damien A. Leach , Nicolas Moser , Naveed Sarwar , Stephen Mangar , Charlotte L. Bevan , Pantelis Georgiou","doi":"10.1016/j.bios.2025.117407","DOIUrl":"10.1016/j.bios.2025.117407","url":null,"abstract":"<div><div>Prostate cancer (PCa) is a highly prevalent disease, causing the second largest amount of male cancer deaths worldwide. Currently, the prostate specific antigen (PSA) test remains the standard serum prognostic and diagnostic monitoring biomarker but it lacks specificity and sensitivity. PSA testing can lead to invasive biopsies which can result in under detection of clinically significant disease and potential overtreatment of indolent disease. Promising circulating biomarkers could facilitate less invasive and more accurate tests, but present challenges in robust quantitation and deployment in clinical settings. This work presents the detection of circulating YAP1 nucleic acid, androgen receptor (AR-FL) and AR-V7 mRNA for PCa prognostics in blood plasma from PCa patients. Sensitive detection of circulating YAP1 nucleic acid, AR-FL and AR-V7 mRNA extracted from PCa clinical samples was achieved with a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay. Optimisation of mRNA extraction methodologies for reliable detection of circulating mRNA for RT-LAMP and RT-qPCR detection took place. Multiplex testing of circulating AR-FL mRNA and YAP1 nucleic acid on an ISFET Lab-on-Chip platform was readily achieved with bio-electronic signal detection taking place within 15 min. Detection of AR-V7 and AR-FL mRNA could also be achieved simultaneously with the handheld device. Evaluation of clinical data indicated that circulating YAP1 nucleic acid presence in extracted RNA from the blood plasma of patients correlated with more advanced clinical cancer staging (<em>p</em> = 0.043) and PSA at diagnosis (<em>p</em> = 0.035). The work presents potential for Point-of-Care detection of circulating mRNA from clinical samples for PCa prognostics.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"281 ","pages":"Article 117407"},"PeriodicalIF":10.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface-engineered nanobeads for regioselective antibody binding: A robust immunoassay platform leveraging catalytic signal amplification 用于区域选择性抗体结合的表面工程纳米珠：利用催化信号放大的强大免疫分析平台

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-04-08 DOI: 10.1016/j.bios.2025.117463

Jounghyun Yoo , Youngsun Kim , Ji Hyun Back , Jawon Shin , Pan Kee Bae , Kyung Mi Park , Myung Kim , Young Hun Seo , Yecheol Bak , Yoon Ho Heo , Jeongyun Heo , Honghwan Choi , Yongju Kim , Sangyoup Lee , Ji Eun Lee , Sohdam Jeong , Jin-Kyoung Yang , Sehoon Kim

{"title":"Surface-engineered nanobeads for regioselective antibody binding: A robust immunoassay platform leveraging catalytic signal amplification","authors":"Jounghyun Yoo , Youngsun Kim , Ji Hyun Back , Jawon Shin , Pan Kee Bae , Kyung Mi Park , Myung Kim , Young Hun Seo , Yecheol Bak , Yoon Ho Heo , Jeongyun Heo , Honghwan Choi , Yongju Kim , Sangyoup Lee , Ji Eun Lee , Sohdam Jeong , Jin-Kyoung Yang , Sehoon Kim","doi":"10.1016/j.bios.2025.117463","DOIUrl":"10.1016/j.bios.2025.117463","url":null,"abstract":"<div><div>Regulating protein interactions and protein corona formation of nanomaterials is crucial for advancing nanomedicine, where surface engineering of nanomaterials plays a pivotal role in precise control over biological interactions. Here, we present a surface-engineered nanoparticle-based immunoassay platform using carboxyl-enriched polystyrene nanobeads (CEPS) with regioselectively controlled antibody-binding properties. Proteomic analysis and theoretical simulation revealed that CEPS has an enhanced Fc-specific binding affinity for immunoglobulins compared to conventional carboxylated polystyrene beads, with a higher surface carboxyl density critically mediating protein interactions. This regioselective antibody binding with unique Fc-specific affinity eliminates the need for complex surface modifications, streamlining the assay process and broadening the applicability across various immunoassay formats. Additionally, incorporating a palladium catalyst within CEPS enables solvent-triggered on-demand catalytic signal amplification using a leucodye substrate, providing a more stable alternative to enzyme-based methods while significantly enhancing detection sensitivity and stability. The platform demonstrated enhanced performance in detecting clinically relevant biomarkers, including C-reactive protein, interferon-gamma, and the receptor-binding domain of SARS-CoV2, achieving lower detection limits and faster response times compared to conventional enzyme-based ELISA systems. Notably, the CEPS-based assay retained catalytic activity for over 140 days at room temperature, underscoring its potential for reliable, long-term use in diverse diagnostic applications.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"281 ","pages":"Article 117463"},"PeriodicalIF":10.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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