Flower-like ytterbium Prussian blue analogue nanozyme with freeze-drying method for colorimetric/photothermal/smartphone triple-mode detection of thiosulfate

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-09-14 DOI:10.1016/j.bios.2025.117997

Wenting Wang , Yupeng Ren , Biao Guan , Hong Cao , Wenling Hu , Lei Li , Longhua Guo , Yanbo Zeng

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引用次数: 0

Abstract

The morphology affects the catalytic activity of nanozymes. This work prepared different ytterbium Prussian blue analogue (PBA) nanozymes with flower-like and rod-like morphologies through changing various amount of K₃[Fe(CN)₆] and Yb(NO₃)₃. Flower-like ytterbium PBA (F-YbFe-PBA) prepared using freeze-drying method and lanthanide-doped strategy with 1.5 mmol K₃[Fe(CN)₆] and Yb(NO₃)₃ had highest peroxidase-like (POD-like) activity among other F-YbFe-PBA nanozymes with freeze-drying method and rod-like YbFe-PBA (R-YbFe-PBA) nanozymes with traditional coprecipitation approach. F-YbFe-PBA exhibited higher POD-like activity than R-YbFe-PBA, which was attributed to the higher surface area and larger pore size of F-YbFe-PBA. F-YbFe-PBA demonstrated superior POD-like performance compared to other PBA nanozymes of SmFe-PBA, PrFe-PBA, MnFe-PBA, and CuFe-PBA. F-YbFe-PBA provided excellent POD-like activity with Michaelis constant of 0.16 mM and V_max value of 6.21 × 10⁻⁸ M s⁻¹ for substrate of 3,3′,5,5′-tetramethylbenzidine (TMB). In the presence of H₂O₂, F-YbFe-PBA catalyzes the decomposition of H₂O₂ to generate reactive hydroxyl radicals. However, thiosulfate (S₂O₃²⁻) has the reducibility capability, effectively suppressing the colorimetric reaction. Therefore, F-YbFe-PBA was successfully utilized to construct a triple-mode selective detection combining colorimetric, photothermal, and smartphone sensing for S₂O₃²⁻ analysis. This triple-mode sensor demonstrated excellent linear detection of S₂O₃²⁻ across a range of 0.1–75 μM through colorimetric, photothermal, and smartphone assays, achieving detection limits of 0.01, 0.01, and 0.03 μM, respectively. Notably, a smartphone-based paper senor with red/blue (R/B) values was developed for real-time and portable detection assay of S₂O₃²⁻. The proposed sensor had high selectivity for S₂O₃²⁻. The F-YbFe-PBA-based sensor was successfully employed for S₂O₃²⁻ detection in tap water and milk samples.

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花状普鲁士蓝类似物纳米酶冻干法比色/光热/智能手机三模检测硫代硫酸盐

纳米酶的形态影响其催化活性。本文通过改变不同量的K3[Fe(CN)6]和Yb(NO3)3，制备了不同形态的普鲁士蓝类似物（yba）纳米酶，分别具有花状和棒状的形态。以1.5 mmol K3[Fe(CN)6]和Yb(NO3)3为原料，冷冻干燥法制备的花状YbFe-PBA纳米酶（F-YbFe-PBA）和传统共沉淀法法制备的棒状YbFe-PBA纳米酶（R-YbFe-PBA）的过氧化物酶样活性最高。F-YbFe-PBA比R-YbFe-PBA表现出更高的pod样活性，这是由于F-YbFe-PBA具有更高的表面积和更大的孔径。与SmFe-PBA、PrFe-PBA、MnFe-PBA和CuFe-PBA等PBA纳米酶相比，F-YbFe-PBA表现出优异的pod样性能。F-YbFe-PBA对3,3',5,5'-四甲基联苯胺（TMB）底物具有良好的pod样活性，米氏常数为0.16 mM， Vmax为6.21 × 10-8 M s-1。在H2O2存在下，F-YbFe-PBA催化H2O2分解生成活性羟基自由基。而硫代硫酸盐（S2O32-）具有还原性，能有效抑制比色反应。因此，我们成功地利用F-YbFe-PBA构建了一种结合比色、光热和智能手机传感的三模选择性检测方法，用于S2O32-分析。通过比色法、光热法和智能手机分析，该三模传感器在0.1 ~ 75 μM范围内对S2O32-进行了良好的线性检测，检测限分别为0.01、0.01和0.03 μM。值得注意的是，开发了一种基于智能手机的红/蓝（R/B）值纸传感器，用于实时和便携式检测S2O32-。该传感器对S2O32-具有较高的选择性。成功地将f - ybfe - pba传感器应用于自来水和牛奶样品中S2O32的检测。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.