Biosensors and Bioelectronics最新文献_第8页

Protein denaturation inspired microchannel-based electrochemiluminescence sensor for formaldehyde detection 基于微通道的蛋白质变性启发电化学发光传感器用于甲醛检测

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-09-13 DOI: 10.1016/j.bios.2024.116778

{"title":"Protein denaturation inspired microchannel-based electrochemiluminescence sensor for formaldehyde detection","authors":"","doi":"10.1016/j.bios.2024.116778","DOIUrl":"10.1016/j.bios.2024.116778","url":null,"abstract":"<div><p>Establishing an effective system to measure formaldehyde (HCHO) content in food is of great significance due to food safety concern. Inspired by the mechanism of HCHO-induced protein denaturation and its effect on ion/molecule transport in nanochannels, a bioinspired microchannel-based electrochemiluminescence (ECL) sensor was constructed for HCHO detection. Benefiting from the water solubility of HCHO, the molecules rapidly spread and enriched at the ethylenediamine (EDA) functionalized microchannel interface. The reaction between EDA and HCHO significantly increased the negative charge density, leading to enhanced electroosmotic flow (EOF). This enhancement resulted in ion concentration depletion at the microchannel tip and a corresponding decrease in ionic current and ECL intensity. The ECL intensity exhibited a linear dependence on the logarithm of HCHO concentration ranging from 1 pg mL<sup>−1</sup> to 100 ng mL<sup>−1</sup>, with a detection limit of 0.26 pg mL<sup>−1</sup>(S/N = 3). The biosensor demonstrated high selectivity, successfully detecting HCHO in shrimp samples. The performance of the bioinspired sensor was confirmed through comparation with existing methods, showcasing its superior sensitivity and reliability. The bioinspired sensor provides robust technical support for HCHO detection, crucial for food safety monitoring. Additionally, the innovative combination of bionics and microchannel-based ECL technology broadens the application range of ECL sensors, marking a significant advancement in the field.</p></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142173250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-vitro blood purification using tiny pinch holographic optical tweezers based on deep learning 基于深度学习的微型夹式全息光镊用于体外血液净化

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-09-12 DOI: 10.1016/j.bios.2024.116781

引用次数: 0

Progress of machine learning-based biosensors for the monitoring of food safety: A review 基于机器学习的食品安全监控生物传感器的进展：综述

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-09-12 DOI: 10.1016/j.bios.2024.116782

{"title":"Progress of machine learning-based biosensors for the monitoring of food safety: A review","authors":"","doi":"10.1016/j.bios.2024.116782","DOIUrl":"10.1016/j.bios.2024.116782","url":null,"abstract":"<div><p>Rapid urbanization and growing food demand caused people to be concerned about food safety. Biosensors have gained considerable attention for assessing food safety due to selectivity, and sensitivity but poor stability inherently limits their application. The emergence of machine learning (ML) has enhanced the efficiency of different sensors for food safety assessment. The ML combined with various noninvasive biosensors has been implemented efficiently to monitor food safety by considering the stability of bio-recognition molecules. This review comprehensively summarizes the application of ML-powered biosensors to investigate food safety. Initially, different detector-based biosensors using biological molecules with their advantages and disadvantages and biosensor-related various ML algorithms for food safety monitoring have been discussed. Next, the application of ML-powered biosensors to detect antibiotics, foodborne microorganisms, mycotoxins, pesticides, heavy metals, anions, and persistent organic pollutants has been highlighted for the last five years. The challenges and prospects have also been deliberated. This review provides a new prospect in developing various biosensors for multi-food contaminants powered by suitable ML algorithms to monitor in-situ food safety.</p></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142225415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Size-tunable transmembrane nanopores assembled from decomposable molecular templates 由可分解分子模板组装而成的尺寸可调跨膜纳米孔

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-09-11 DOI: 10.1016/j.bios.2024.116780

{"title":"Size-tunable transmembrane nanopores assembled from decomposable molecular templates","authors":"","doi":"10.1016/j.bios.2024.116780","DOIUrl":"10.1016/j.bios.2024.116780","url":null,"abstract":"<div><p>Transmembrane nanopores, as key elements in molecular transport and single-molecule sensors, are assembled naturally from multiple monomers in the presence of lipid bilayers. The nanopore size, especially the precise diameter of the inner space, determines its sensing targets and further biological application. In this paper, we introduce a template molecule-aided assembly strategy for constructing size-tunable transmembrane nanopores. Inspired by the barrel-like structure, similar to many transmembrane proteins, cyclodextrin molecules of different sizes are utilized as templates and modulators to assemble the α-helical barreled peptide of polysaccharide transporters (Wza). The functional nanopores assembled by this strategy possess high biological and chemical activity and can be inserted into lipid bilayers, forming stable single channels for single-molecule sensing. After enzyme digestion, the cyclodextrins on protein nanopores can be degraded, and the remaining nontemplate transmembrane protein nanopores can also preserve the integrity of their structure and function. The template molecule-aided assembly strategy employed a simple and convenient method for fully artificially synthesizing transmembrane protein nanopores; the pore size is completely dependent on the size of the template molecule and controllable, ranging from 1.1 to 1.8 nm. Furthermore, by chemically synthesized peptides and modifications, the pore function is easily modulated and does not involve the cumbersome genetic mutations of other biological techniques.</p></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142198863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-responsive two-photon probe for specific lipid droplets near-infrared fluorescence imaging in the brain of epileptic mice 用于癫痫小鼠脑内特异性脂滴近红外荧光成像的双响应双光子探针

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-09-11 DOI: 10.1016/j.bios.2024.116774

{"title":"Dual-responsive two-photon probe for specific lipid droplets near-infrared fluorescence imaging in the brain of epileptic mice","authors":"","doi":"10.1016/j.bios.2024.116774","DOIUrl":"10.1016/j.bios.2024.116774","url":null,"abstract":"<div><p>Abnormal lipid metabolism in glial cells is a key pathological feature of epilepsy. The identification of lipid droplets (LDs) is essential for investigating lipid metabolism, disease progression, and potential therapeutic interventions. Two-photon imaging technology enables real-time visualization of the spatial distribution and temporal dynamics of LDs in epilepsy models. In this study, we developed a novel two-photon excited dual-responsive near-infrared fluorescent probe, <strong>CabA</strong>, based on viscosity and polarity, to monitor dynamic changes in LDs. The fluorescence of <strong>CabA</strong> at 670 nm exhibits a significant increase in response to low polarity and high viscosity due to the twisted intramolecular charge transfer and intramolecular charge transfer mechanisms. The LDs-targeting capability of <strong>CabA</strong> at the cellular level and the process of LDs generation between neurons and astrocytes during the pathological advancement of epilepsy have been validated. In situ synchronous imaging experiments in epileptic and normal mice using <strong>CabA</strong> revealed abnormal LDs accumulation in the brain during seizures. Two-photon fluorescence imaging further demonstrated LDs accumulation in the brains of epileptic mice at a penetration depth of 100 μm. This study offers a valuable tool for enhancing the understanding of LDs in physiological and pathological processes, potentially aiding in the early diagnosis of epilepsy.</p></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142225419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanometal surface energy transfer-based lateral flow immunoassay for T2 toxin detection 基于纳米表面能量转移的 T2 毒素检测侧流免疫分析法

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-09-11 DOI: 10.1016/j.bios.2024.116779

引用次数: 0

“Partner” cellulose gel with “dialysis” function: Achieve the integration of filtration-enrichment-SERS detection 具有 "透析 "功能的 "伙伴 "纤维素凝胶：实现过滤-富集-SERS 检测一体化

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-09-11 DOI: 10.1016/j.bios.2024.116775

{"title":"“Partner” cellulose gel with “dialysis” function: Achieve the integration of filtration-enrichment-SERS detection","authors":"","doi":"10.1016/j.bios.2024.116775","DOIUrl":"10.1016/j.bios.2024.116775","url":null,"abstract":"<div><p>Hydrogel and aerogel materials have garnered significant attention in constructing effective surface-enhanced Raman spectroscopy (SERS) substrates due to their excellent adsorption capabilities, high specific surface area, and abundant chemical groups. However, in liquids with complex compositions, non-specific adsorption of macromolecules can lead to surface scaling and pore clogging of the substrate material, limiting the selective enrichment and SERS detection of target molecules. To address this, an innovative aerogel-chimeric hydrogel material (CH@S-CNF/SA/Ag NPs) was developed. The aerogel component, with its high specific surface area and electronegative properties, functions as a SERS “chip” for adsorption and detection of target molecules. Simultaneously, the mesoporous structure of the hydrogel “shell” effectively filters macromolecules from the solution. These CH@S-CNF/SA/Ag NPs were utilized as SERS substrate materials for detecting urine from healthy individuals and patients with chronic kidney disease stage 5 (CKD5). When combined with machine learning algorithms, the detection accuracy reached 99.50%. This work represents a significant advancement in the specific adsorption and SERS detection of small molecules in complex biological samples such as urine and blood.</p></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142198864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Explainable artificial intelligence-driven prostate cancer screening using exosomal multi-marker based dual-gate FET biosensor 利用基于外泌体多标记物的双栅 FET 生物传感器进行可解释的人工智能驱动的前列腺癌筛查

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-09-10 DOI: 10.1016/j.bios.2024.116773

{"title":"Explainable artificial intelligence-driven prostate cancer screening using exosomal multi-marker based dual-gate FET biosensor","authors":"","doi":"10.1016/j.bios.2024.116773","DOIUrl":"10.1016/j.bios.2024.116773","url":null,"abstract":"<div><p>Prostate Imaging Reporting and Data System (PI-RADS) score, a reporting system of prostate MRI cases, has become a standard prostate cancer (PCa) screening method due to exceptional diagnosis performance. However, PI-RADS 3 lesions are an unmet medical need because PI-RADS provides diagnosis accuracy of only 30–40% at most, accompanied by a high false-positive rate. Here, we propose an explainable artificial intelligence (XAI) based PCa screening system integrating a highly sensitive dual-gate field-effect transistor (DGFET) based multi-marker biosensor for ambiguous lesions identification. This system produces interpretable results by analyzing sensing patterns of three urinary exosomal biomarkers, providing a possibility of an evidence-based prediction from clinicians. In our results, XAI-based PCa screening system showed a high accuracy with an AUC of 0.93 using 102 blinded samples with the non-invasive method. Remarkably, the PCa diagnosis accuracy of patients with PI-RADS 3 was more than twice that of conventional PI-RADS scoring. Our system also provided a reasonable explanation of its decision that TMEM256 biomarker is the leading factor for screening those with PI-RADS 3. Our study implies that XAI can facilitate informed decisions, guided by insights into the significance of visualized multi-biomarkers and clinical factors. The XAI-based sensor system can assist healthcare professionals in providing practical and evidence-based PCa diagnoses.</p></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0956566324007796/pdfft?md5=1d5599fb5244bc3980c2961fe44eb983&pid=1-s2.0-S0956566324007796-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142225420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microdroplet-enhanced chip platform for high-throughput immunotherapy marker screening from extracellular vesicle RNAs and membrane proteins 从细胞外囊泡 RNA 和膜蛋白中高通量筛选免疫疗法标记物的微滴增强芯片平台

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-09-10 DOI: 10.1016/j.bios.2024.116748

引用次数: 0

Universal sulfatase-based chemiluminescence biosensing platform: Validation via AFP detection in clinical blood samples 基于硫酸酯酶的通用化学发光生物传感平台：通过检测临床血液样本中的甲胎蛋白进行验证

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-09-10 DOI: 10.1016/j.bios.2024.116771

引用次数: 0