Biosensors and Bioelectronics最新文献_第8页

From biosensing to perception: Collaborative few-shot learning for explainable digital biomarker identification in high-dimensional biomedical spectra 从生物传感到感知：高维生物医学光谱中可解释的数字生物标志物识别的协作少镜头学习。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-17 DOI: 10.1016/j.bios.2025.117980

Junhan Yang , Chen Shen , Ningtao Cheng

{"title":"From biosensing to perception: Collaborative few-shot learning for explainable digital biomarker identification in high-dimensional biomedical spectra","authors":"Junhan Yang , Chen Shen , Ningtao Cheng","doi":"10.1016/j.bios.2025.117980","DOIUrl":"10.1016/j.bios.2025.117980","url":null,"abstract":"<div><div>The application of <em>in vitro</em> diagnostic biosensors for early cancer detection remains challenging due to the insufficient representation by a few molecular biomarkers. Digital biomarkers promise comprehensive disease phenotyping but face constraints of clinical data scarcity and obstacles of limited generalization. Here, we introduce Coupled Explainable Artificial Intelligence Recursive (CEAIR) learning, a computational framework that integrates computer vision and cooperative game theory for interpretable few-shot learning, enabling the extraction of domain-relevant digital biomarkers from high-dimensional surface-enhanced Raman spectroscopy biosensor data of limited serum samples. Applied to hepatocellular carcinoma detection, CEAIR-derived digital biomarkers significantly outperform circulating molecular biomarkers, achieving area under the curve values consistently exceeding 0.97 across multiple independent classifiers built with classic machine learning algorithms and demonstrating strong generalization upon external validation. Our findings underscore CEAIR's capacity to overcome fundamental limitations in generating clinically meaningful diagnostic knowledge from high-dimensional, small-sample biosensor data, learning reliable digital biomarkers for robust, non-invasive, and timely diagnosis of complex diseases.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 117980"},"PeriodicalIF":10.5,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145084722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microneedle-based detection of nucleic acids in interstitial fluid: A review 基于微针的间质液核酸检测研究进展

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-17 DOI: 10.1016/j.bios.2025.117996

Hongbo Liu , Haotian Zheng , Niancai Peng , Fei Hu

{"title":"Microneedle-based detection of nucleic acids in interstitial fluid: A review","authors":"Hongbo Liu , Haotian Zheng , Niancai Peng , Fei Hu","doi":"10.1016/j.bios.2025.117996","DOIUrl":"10.1016/j.bios.2025.117996","url":null,"abstract":"<div><div>Traditional nucleic acid detection methods rely on external sampling and complex, multi-step offline analyses, which result in long turnaround times and require specialized laboratory infrastructure. Recent advancements in microneedle (MN)-based technologies have transformed interstitial fluid (ISF) sampling by enabling minimally invasive and real-time monitoring of biomarkers. This review systematically discusses the development of MN-mediated ISF nucleic acid detection platforms, with emphasis on four key areas: (1) The principles and optimization of MN-based ISF sampling, encompassing fabrication techniques and extraction mechanisms such as negative pressure, swelling, and capillary action; (2) Nucleic acid enrichment and amplification strategies specifically adapted for ISF, including isothermal amplification and hybridization-based methods; (3) The development and targeting MN-based nucleic acid sensing tools; and (4) Medical translation and continuous monitoring pose challenges. This review synthesizes recent progress, highlights technical bottlenecks, and offers insights to support the translational development of clinically viable MN-based ISF nucleic acid detection systems for precision diagnostics and chronic disease surveillance.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 117996"},"PeriodicalIF":10.5,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrabright magneto-fluorescent nanoparticles-enhanced lateral flow immunoassay for ultrasensitive detection of Escherichia coli O157:H7 超亮磁荧光纳米颗粒增强侧流免疫分析法用于超灵敏检测大肠杆菌O157:H7

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-17 DOI: 10.1016/j.bios.2025.118011

Xiaolong Wang , Yuhao Wu , Wenjing Li , Jiayi Sun , Juan Peng , Yu Su , Yuankui Leng , Yonghua Xiong , Xiaolin Huang

{"title":"Ultrabright magneto-fluorescent nanoparticles-enhanced lateral flow immunoassay for ultrasensitive detection of Escherichia coli O157:H7","authors":"Xiaolong Wang , Yuhao Wu , Wenjing Li , Jiayi Sun , Juan Peng , Yu Su , Yuankui Leng , Yonghua Xiong , Xiaolin Huang","doi":"10.1016/j.bios.2025.118011","DOIUrl":"10.1016/j.bios.2025.118011","url":null,"abstract":"<div><div>Rapid and sensitive detection of <em>Escherichia coli</em> O157:H7 in complex food matrices is critical for ensuring food safety and public health. Herein, an innovative lateral flow immunoassay (LFIA) platform was developed for detecting <em>E. coli</em> O157:H7, utilizing a probe of magneto-fluorescent nanoparticles and smartphone interpretation. An ultrabright magneto-fluorescent nanoparticle, Fe<sub>3</sub>O<sub>4</sub>@mSiO<sub>2</sub>@rQDs, was first prepared by high-density loading of red-emitting quantum dots (rQDs) in a core-shell template of Fe<sub>3</sub>O<sub>4</sub> with a dendritic mesoporous silica shell. Subsequently, a metal-polyphenol network coating composed of Zr<sup>4+</sup> and tannic acid (TA) was applied to enhance the fluorescent stability and water dispersibility of Fe<sub>3</sub>O<sub>4</sub>@mSiO<sub>2</sub>@rQDs, as well as to facilitate efficient and repeatable antibody immobilization. By leveraging immunomagnetic separation-based target enrichment and the high-intensity fluorescent signal output of Fe<sub>3</sub>O<sub>4</sub>@mSiO<sub>2</sub>@rQDs@TA, the developed LFIA achieved rapid and sensitive detection of <em>E. coli</em> O157:H7, with a detection limit as low as 2.12 × 10<sup>2</sup> CFU mL<sup>−1</sup>. Notably, the FSQT-LFIA demonstrated the capability to detect bacterial concentrations down to single-cell levels following a brief pre-enrichment period. Moreover, the FSQT-LFIA exhibited excellent accuracy and high reliability in detecting <em>E. coli</em> O157:H7 across diverse food matrices, including skimmed milk, lettuce, apple juice, and chicken meat, with average recovery rates ranging from 85 % to 110 %. Collectively, our FSQT-LFIA platform presents a promising alternative for ultrasensitive and rapid screening of foodborne pathogens in a variety of complex food samples.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 118011"},"PeriodicalIF":10.5,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR-integrated nanoconfined interparticle catalytic hairpin assembly for enhanced dual-mode SARS-CoV-2 detection in wastewater crispr集成纳米颗粒间催化发夹组件用于增强废水中SARS-CoV-2双模检测

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-17 DOI: 10.1016/j.bios.2025.118008

Xiaoxi Zheng , Shuo Yao , Caihong Yin , Huamin Zhao , Jun Wang , Tan Su , Hang Li , Juan Wang , Chao Zhao

{"title":"CRISPR-integrated nanoconfined interparticle catalytic hairpin assembly for enhanced dual-mode SARS-CoV-2 detection in wastewater","authors":"Xiaoxi Zheng , Shuo Yao , Caihong Yin , Huamin Zhao , Jun Wang , Tan Su , Hang Li , Juan Wang , Chao Zhao","doi":"10.1016/j.bios.2025.118008","DOIUrl":"10.1016/j.bios.2025.118008","url":null,"abstract":"<div><div>Accurate monitoring of pathogenic viruses in wastewater is critical for early outbreak and risk assessment. This study presented a novel biosensing platform that combined an interparticle magnetic covalent organic framework (MCOF)-assisted mismatched catalytic hairpin assembly (iMMCHA) with CRISPR/Cas12a-activated colorimetric-photothermal dual-mode detection of SARS-CoV-2 RNA. The system strategically immobilized CHA reactants (H1 and mismatched H2) on separate MCOF nanoparticles, creating a spatially confined and collision-enhanced interparticle MCHA that achieved 270-fold higher local reactant concentration and 20-min faster kinetics than solution-phase CHA. Upon target recognition, the iMMCHA system generated dsDNA activators that triggered Cas12a-mediated cleavage of ssDNA linkers on magnetic bead-glucose oxidase conjugates. This cleavage event reduced the TMB-oxidizing activity of the magnetically isolated integrated enzyme system, producing inversely correlated colorimetric and photothermal signals. This iMMCHA-CRISPR dual-mode assay allowed for the rapid and sensitive detection of SARS-CoV-2 pseudovirus in sanitary wastewater samples, with detection limits of 100 and 120 copies/<em>μ</em>L (colorimetric mode) and 100 and 140 copies/<em>μ</em>L (photothermal mode) for S and N genes, respectively. This work established a powerful platform for aqueous environmental virus monitoring that combined the specificity of CRISPR with the signal enhancement and kinetics acceleration of nanoconfined interparticle CHA and the reliability of dual-mode detection.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 118008"},"PeriodicalIF":10.5,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145096313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Imidazole-enhanced luminol/H2O2 chemiluminescent biosensing for SARS-CoV-2 nucleocapsid protein with enzymatic regulation of hemin switch 咪唑增强鲁米诺/H2O2化学发光生物传感及酶促血红蛋白开关

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-16 DOI: 10.1016/j.bios.2025.118002

Hang Ao , Jinshan Xiong , Wencheng Xiao , Wenrui Hu , Jie Wu , Huangxian Ju

{"title":"Imidazole-enhanced luminol/H2O2 chemiluminescent biosensing for SARS-CoV-2 nucleocapsid protein with enzymatic regulation of hemin switch","authors":"Hang Ao , Jinshan Xiong , Wencheng Xiao , Wenrui Hu , Jie Wu , Huangxian Ju","doi":"10.1016/j.bios.2025.118002","DOIUrl":"10.1016/j.bios.2025.118002","url":null,"abstract":"<div><div>Owing to the continuous mutations of the coronavirus, the sensitive and accurate detection of the SARS-CoV-2 nucleocapsid protein is still necessary in clinical diagnosis. Aim to the challenge of sensitivity in chemiluminescence (CL) imaging detection and the need for high-throughput clinical screening, this work used imidazole (Im) as an enhancer for hemin-DNA/luminol/H<sub>2</sub>O<sub>2</sub> CL system to propose a CL imaging method for SARS-CoV-2 nucleocapsid protein detection. In this system Im could reduce the hemin-DNA catalyzed oxidation energy of luminol by H<sub>2</sub>O<sub>2</sub> to promote the CL emission through the accelerated breaking of O-O bond of dianionic cyclic peroxide. The enhancement mechanism was demonstrated with theoretical calculations and several characterization techniques. By combining the proximity-induced initiator release with immunological recognition, an initiator-triggered DNA nanomachine was designed to achieve the release of abundant primers for activating hemin-DNA switch. This signal amplification strategy led to strong CL emission for highly sensitive imaging detection of the target. The Im-enhanced hemin-DNA/luminol/H<sub>2</sub>O<sub>2</sub> CL imaging assay exhibited a linear detection range over 4 orders of magnitude with a detection limit down to 2.75 pg/mL for SARS-CoV-2 nucleocapsid protein, exhibiting promising potential of both Im sensitization and the proposed DNA nanomachine to improve the sensitivity of high-throughput CL biosensing for the early diagnosis and clinical screening of the coronavirus and other diseases.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 118002"},"PeriodicalIF":10.5,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145096267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Calix[4]arene-based wettability interface sensor for rapid ATP detection 一种用于ATP快速检测的杯状[4]芳烃润湿性界面传感器。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-16 DOI: 10.1016/j.bios.2025.118001

Haonan Qu , Haifan Zhang , Cuiguang Ma , Qiang He , Ehsan Bahojb Noruzi , Xiangcheng Li , Guang Li , Haibing Li

引用次数: 0

Confined and fluid entropy-driven strand displacement reaction on red blood cell membrane for rapid and efficient detection of MicroRNA 红血球膜上的受限流体熵驱动链位移反应，用于快速高效检测MicroRNA

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-16 DOI: 10.1016/j.bios.2025.118003

Bo Wang , Wenmeng Li , Gaoyang Lu , Jialin Wen , Menghui Wang , Longwei Bai , Yin Wang , Xueyuan Huang , Mei Wen , Shuangyan Huan , Guoliang Ke , Xiao-Bing Zhang , Mei Chen

{"title":"Confined and fluid entropy-driven strand displacement reaction on red blood cell membrane for rapid and efficient detection of MicroRNA","authors":"Bo Wang , Wenmeng Li , Gaoyang Lu , Jialin Wen , Menghui Wang , Longwei Bai , Yin Wang , Xueyuan Huang , Mei Wen , Shuangyan Huan , Guoliang Ke , Xiao-Bing Zhang , Mei Chen","doi":"10.1016/j.bios.2025.118003","DOIUrl":"10.1016/j.bios.2025.118003","url":null,"abstract":"<div><div>Entropy-driven Strand Displacement Reaction (ESDR) serves as a fundamental tool for designing DNA signal-amplified biosensors, demonstrating significant potential in early disease diagnosis. Unfortunately, most ESDR systems still face problems such as complex design, long reaction time (typically several hours), low efficiency, and poor stability in complex biological environments. Inspired by the natural fluid cell membrane, we employed the simply prepared red blood cell (RBC) membrane as a carrier to design a confined and fluid RBC-ESDR system for rapid and efficient detection of microRNA. RBC-ESDR is convenient and efficient in loading different probes and adjusting the probe ratio. By utilizing its spatial confinement effect and membrane fluidity, the local concentration of the probes and the collision efficiency between the probes are significantly improved, thus greatly accelerating the reaction kinetics. Compared to conventional free-ESDR system relying on free diffusion and random collision, RBC-ESDR system achieves higher reaction speed (increased by 6 times) and higher sensitivity (2 orders of magnitude), allowing rapid and sensitive detection of microRNA in complex biological matrices, exhibiting economic, rapid, and sensitive clinical diagnostic potential.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118003"},"PeriodicalIF":10.5,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145120423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Caspase 8-activated bioluminescence probe for in vivo imaging of programmable cell death Caspase 8激活的生物发光探针用于程序性细胞死亡的体内成像。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-16 DOI: 10.1016/j.bios.2025.118004

Zihan Yuan , Qiaochu Jiang , Jingyang Huang , Xiaoyang Liu , Xiaotong Cheng , Yang Yang , Hongzhe Yan , Xianbao Sun , Gaolin Liang

{"title":"Caspase 8-activated bioluminescence probe for in vivo imaging of programmable cell death","authors":"Zihan Yuan , Qiaochu Jiang , Jingyang Huang , Xiaoyang Liu , Xiaotong Cheng , Yang Yang , Hongzhe Yan , Xianbao Sun , Gaolin Liang","doi":"10.1016/j.bios.2025.118004","DOIUrl":"10.1016/j.bios.2025.118004","url":null,"abstract":"<div><div>Programmable cell death, including apoptosis and pyroptosis, is central to physiological homeostasis, with Caspase-8 serving as a pivotal molecular switch. However, no Caspase-8-specific self-illuminating bioluminescence probe has been reported for in vivo imaging of these pathways. Here, we report a Caspase-8-activated bioluminescence probe Ac-Ile-Glu-Thr-Asp-<sub>D</sub>-Aminoluciferin (<strong>Ac-IETD-Amluc</strong>). In vitro experiments confirmed that <strong>Ac-IETD-Amluc</strong> was efficiently and specifically cleaved by Caspase-8 to release bioluminescent Amluc, with a linear relationship of bioluminescence versus Caspase-8 concentration (limit of detection: 0.082 g/L). Upon cisplatin-induced apoptosis and H<sub>2</sub>TCPP-sensitized laser irradiation-induced pyroptosis in firefly luciferase-transfected 4T1 (fLuc-4T1) cells, <strong>Ac-IETD-Amluc</strong> treatment led to cell bioluminescence signals peaking at 40 min (3.3-fold higher than the inhibitor control group) and 10 min (3.7-fold higher than the inhibitor control group), respectively. In fLuc-4T1 tumor-bearing mice, bioluminescence intensities within tumors peaked at 10 min post-injection of <strong>Ac-IETD-Amluc</strong>, with 4.2-fold (apoptosis group) and 6.8-fold (pyroptosis group) increases compared to inhibitor control groups. Given its superior capacity for real-time monitoring of cell death pathways in vivo, this probe is anticipated to be applied for diagnosing diseases involving dysregulated cell death, such as cancers, neurodegenerative disorders, and inflammatory syndromes.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 118004"},"PeriodicalIF":10.5,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Front-illuminated surface plasmon resonance biosensor for the study of light-responsive proteins and their interactions 用于研究光响应蛋白及其相互作用的前照表面等离子体共振生物传感器

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-16 DOI: 10.1016/j.bios.2025.117998

Giusy Finocchiaro , Aditya Suresh Chaudhari , Tomáš Špringer , Kateřina Králová , Karel Chadt , Erika Hemmerová , Jan Bukáček , Phuong Ngoc Pham , Aditi Chatterjee , Bohdan Schneider , Gustavo Fuertes , Jiří Homola

{"title":"Front-illuminated surface plasmon resonance biosensor for the study of light-responsive proteins and their interactions","authors":"Giusy Finocchiaro , Aditya Suresh Chaudhari , Tomáš Špringer , Kateřina Králová , Karel Chadt , Erika Hemmerová , Jan Bukáček , Phuong Ngoc Pham , Aditi Chatterjee , Bohdan Schneider , Gustavo Fuertes , Jiří Homola","doi":"10.1016/j.bios.2025.117998","DOIUrl":"10.1016/j.bios.2025.117998","url":null,"abstract":"<div><div>Light-responsive proteins are involved in a wide range of essential physiological processes in bacteria, plants, and animals. Engineered light-responsive proteins have also emerged as prospective tools in biotechnology and biomedicine. These proteins are often characterized by short-lived lit states and the need for continuous illumination to reach photostationary states. Therefore, developing methods for studying light-responsive proteins and their interactions under illumination represents an important research goal. Here, we report on a novel front-illuminated surface plasmon resonance (fiSPR) biosensor for monitoring interactions involving light-responsive proteins. The fiSPR biosensor combines the optical platform based on the Kretschmann geometry with advanced transparent microfluidics and an additional light module, enabling <em>in situ</em> illumination of the liquid sample in contact with the SPR chip. We apply the fiSPR biosensor to study the blue light-responsive transcription factor EL222, which recovers to the dark state in a few seconds and plays an important role in the optogenetic control of gene expression. Specifically, we determine the rate and equilibrium constants for EL222 dimerization and DNA binding. The results support the hypothesis that EL222 dimerizes prior to binding DNA. In addition, we provide evidence of the interaction between an interleukin receptor modified with a photocaged tyrosine (IL-20R2-Y70NBY) and its cytokine ligand (IL-24) only upon UV illumination. Overall, this study demonstrates the versatility of the developed fiSPR biosensor for monitoring biomolecular interactions involving both natural and engineered light-responsive proteins, particularly those featuring short lit-state lifetimes.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 117998"},"PeriodicalIF":10.5,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From lab to field: Supramolecular probe for gossypol real-time quantification 从实验室到现场：棉酚实时定量的超分子探针。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-16 DOI: 10.1016/j.bios.2025.118005

Linxiao Bai , Kehong Jiang , Tianhua Ma , Wei Guo , Ran Yang , Yuanqiang Sun , Zhaohui Li

{"title":"From lab to field: Supramolecular probe for gossypol real-time quantification","authors":"Linxiao Bai , Kehong Jiang , Tianhua Ma , Wei Guo , Ran Yang , Yuanqiang Sun , Zhaohui Li","doi":"10.1016/j.bios.2025.118005","DOIUrl":"10.1016/j.bios.2025.118005","url":null,"abstract":"<div><div>The extensive use of gossypol-containing cottonseed products in food and animal feed presents considerable health hazards to humans and livestock. Despite the pressing demand for rapid and user-friendly detection methods to monitor gossypol contamination, existing solutions are insufficient. To address this critical need, we introduce a novel supramolecular fluorescent probe that exploits the strong binding ability between human albumin serum (HSA) and gossypol for sensitive and selective detection of gossypol in food samples. The probe incorporates a merocyanine-based disaggregation-induced emission (DIE) fluorescent dye <strong>MCID</strong>, which forms a stable supramolecular <strong>MCID@HSA</strong> complex upon encapsulation by HSA. Upon introducing gossypol, specific host-guest interactions within the supramolecular probe resulted in a marked fluorescence enhancement at 614 nm. This facilitated the rapid and quantitative detection of gossypol, achieving a detection limit as low as 13.7 nM and a rapid response time of 10 s. The probe demonstrated excellent selectivity and anti-interference capabilities, ensuring reliable performance in complex matrices. We further engineered this technology into a smartphone-based colorimetric detection system for gossypol detection. This approach obviates the need for expensive instrumentation, providing an accessible alternative for gossypol quantification. The supramolecular probe was effectively utilized for the quantification of trace amounts of gossypol in real samples, such as cottonseed oil and cottonseed meal, yielding satisfactory recovery rates and reproducibility. This innovative approach integrates high analytical performance with practical applicability, providing an essential solution for food safety monitoring.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 118005"},"PeriodicalIF":10.5,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145084688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0