Biosensors and Bioelectronics最新文献

{"title":"Diagnosis and classification of infective endocarditis via efficient serum metabolic fingerprint analysis","authors":"Ayizekeranmu Yiming ,&nbsp;Xinxin Ma ,&nbsp;Xiran Chen ,&nbsp;Zonghui Chen ,&nbsp;Shouzhi Yang ,&nbsp;Jinlong Zhao ,&nbsp;Yinkai Ni ,&nbsp;Cheng Zhang ,&nbsp;Liang Fu ,&nbsp;Ruoxin Wang ,&nbsp;Litao Tang ,&nbsp;Feng Li ,&nbsp;Lin Huang ,&nbsp;Kun Qian ,&nbsp;Yuanyi Zheng","doi":"10.1016/j.bios.2026.118425","DOIUrl":"10.1016/j.bios.2026.118425","url":null,"abstract":"<div><div>Infective endocarditis (IE) continues to pose significant clinical challenges as a life-threatening condition associated with 30 % mortality. The current diagnostic criteria, the 2023 Duke-International Society for Cardiovascular Infectious Diseases (ISCVID) criteria, present diagnostic challenges due to complex processes. Blood culture remains a cornerstone of IE diagnosis, enabling identification of the causative microorganism and guiding targeted antibiotic therapy. However, results typically take 2–5 days, significantly delaying critical treatment decisions. To overcome these limitations, we developed a nanoparticle-enhanced laser desorption/ionization mass spectrometry (NPELDI MS) platform capable of acquiring serum metabolic fingerprints (SMFs). When integrated with machine learning algorithms, this platform achieves accurate IE diagnosis (area under the curve (AUC) = 0.882) and rapid streptococcal classification within 10 min. Notably, our platform enables simultaneous IE diagnosis and classification via a single assay free of culture process. This integrated approach addresses the critical unmet need in IE management, offering transformative potential for timely therapeutic decision-making and improved patient outcomes.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"299 ","pages":"Article 118425"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146076606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrated pressure-based lateral flow immunoassay for microliter whole-blood AFP detection at the point of care","authors":"Gang Tang ,&nbsp;Ke Liu ,&nbsp;Lingying Xia ,&nbsp;Tianhao Chen ,&nbsp;Andrew Liman ,&nbsp;Qiao Feng ,&nbsp;Rongxing Zhou","doi":"10.1016/j.bios.2026.118398","DOIUrl":"10.1016/j.bios.2026.118398","url":null,"abstract":"<div><div>Alpha-fetoprotein (AFP) is a widely used biomarker for the diagnosis and postoperative surveillance of hepatocellular carcinoma (HCC). However, current AFP assays rely on centralized laboratories, bulky instrumentation, and labor-intensive sample pretreatment, limiting their applicability in point-of-care and home-based settings. Here, we present an in vitro diagnostic device for quantitative AFP detection in whole blood that integrates a lateral flow immunoassay (LFIA) with a portable pressure meter (PM-LFIA). The platform employs a pressure-based signal readout using nanozyme-catalyzed gas generation to enable sensitive quantification while reducing optical and matrix-related interference. Compared with conventional methods, the PM-LFIA platform achieves a 35-fold reduction in required sample volume and a 215-fold improvement in detection limit. The platform enables AFP detection from 1 μL of unprocessed whole blood within 30 min, supporting point-of-care applications where sample volume is limited. The feasibility of this approach was validated by direct quantification of AFP in 30 whole blood samples, with results consistent with hospital-based measurements. In addition, the applicability of the PM-LFIA platform was demonstrated for other cancer biomarkers, including CEA and CA199. Although environmental sensitivity and device calibration remain challenges, this work demonstrates an integrated and practical strategy for quantitative biomarker detection in whole blood using a portable, low-sample-volume format.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"299 ","pages":"Article 118398"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146049225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptide-responsive photonic hydrogels integrated with deep learning assistance for early MMP-9 detection","authors":"Yan Wang ,&nbsp;Mingyi Liu ,&nbsp;Xufang Liu ,&nbsp;Dongxiao Hao ,&nbsp;Yuzhu Wang ,&nbsp;Yunfan Cai ,&nbsp;Jing Li ,&nbsp;Xiaoyu Miao ,&nbsp;Yifan Zhang ,&nbsp;Xiaojian Yang ,&nbsp;Yongkang Bai ,&nbsp;Chen Wang ,&nbsp;Franklin R. Tay ,&nbsp;Conrado Aparicio ,&nbsp;Yao Zhao ,&nbsp;Lina Niu","doi":"10.1016/j.bios.2026.118444","DOIUrl":"10.1016/j.bios.2026.118444","url":null,"abstract":"<div><div>Matrix metalloproteinase-9 (MMP-9) is crucial for extracellular matrix remodeling, and its dysregulation is associated with inflammatory diseases and different forms of cancer. Conventional MMP-9 detection methods such as enzyme-linked immunosorbent assay (ELISA) are limited by complexity, expensive equipment, and lengthy antibody incubation times. These limitations prevent their use in point-of-care testing. An MMP-9 responsive photonic crystal (PC/PEG-M9SP) hydrogel has been developed to address these challenges. The hydrogel is synthesized from 4-arm polyethylene glycol-acrylate and an MMP-9 sensitive peptide via Michael-type addition reaction. Upon MMP-9-specific enzymatic cleavage, the hydrogel undergoes a structural reconfiguration, resulting in a distinct color shift. Integrated with a deep learning-based smartphone app, this platform enables both visual and quantitative detection within 10 min, achieving high sensitivity (10.60 nm mL/ng) and a detection limit of 0.62 ng/mL. Validation in complex biological fluids demonstrated strong concordance with ELISA, confirming the analytical reliability of the hydrogel. This system provides a rapid, portable, and cost-effective solution for accurate MMP-9 detection, with strong potential for clinical and point-of-care applications.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"299 ","pages":"Article 118444"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146076543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular-recognition architectures and emerging probe strategies for neurotransmitter chemical sensing","authors":"Jiaxue Wu ,&nbsp;Yifan Da ,&nbsp;Lindong Xu ,&nbsp;Runqiao Yang ,&nbsp;Hanshu Li ,&nbsp;Xiaoya Zhou ,&nbsp;Yang Tian ,&nbsp;Yi Zhou","doi":"10.1016/j.bios.2026.118445","DOIUrl":"10.1016/j.bios.2026.118445","url":null,"abstract":"<div><div>Monitoring neurotransmitter dynamics in the brain with high spatial and temporal fidelity is essential for understanding neural circuit function and dysfunction. Although detection technologies have advanced, the molecular recognition elements that initiate sensing still largely define selectivity, speed, and in vivo performance. Here we focus on rapidly evolving molecular-recognition architectures for small-molecule neurotransmitter probes. Moving beyond simple lock-and-key motifs, we compare receptor-inspired binding sites, reaction-driven chemistries, conformational switches, supramolecular host–guest complexes, and engineered proteins through four performance criteria: molecular selectivity, response speed, signal-reporting mechanisms, and in vivo stability. We also highlight how these architectures interface with optical and electrochemical readouts to enable real-time measurements in complex tissue. By framing recent advances within a common structure–performance landscape, this Perspective provides a chemistry-centric guide for designing next-generation molecular tools to interrogate neurotransmitter signaling in the brain.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"299 ","pages":"Article 118445"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146111828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bulge DNA-driven CRISPR/Cas12a dynamic activation circuit enables highly sensitive and versatile biosensing","authors":"Luyu Wei ,&nbsp;Ziqi Cheng ,&nbsp;Mingrui Xu ,&nbsp;Hengye Chen ,&nbsp;Wei Lan ,&nbsp;Wanjun Long ,&nbsp;Yuanbin She ,&nbsp;Haiyan Fu","doi":"10.1016/j.bios.2026.118412","DOIUrl":"10.1016/j.bios.2026.118412","url":null,"abstract":"<div><div>CRISPR/Cas12a has emerged as an innovative biosensing tool; however, its intrinsically linear accumulation of cleavage signals limits detection sensitivity. Herein, we report a bulge DNA (BD)-driven CRISPR/Cas12a dynamic activation circuit, termed CBD, as a highly sensitive and versatile biosensing platform for both nucleic acid and non-nucleic acid targets. The BD structure was rationally engineered to undergo programmable structural and functional transformation upon bulge degradation, thereby initiating exponential, self-amplifying activation of the Cas12a circuit. For nucleic acid targets, direct Cas12a recognition triggers BD cleavage and a positive feedback loop, enabling highly sensitive detection with a limit of 14 CFU/mL for methicillin-resistant <em>Staphylococcus aureus</em>. For non-nucleic acid targets, a universal single-stranded DNA activator was linked to the aptamer-complementary strand, enabling target-responsive release and subsequent initiation of the CBD system without altering the crRNA or BD sequence. This strategy enabled the detection of pesticides and mycotoxins at the picogram-per-milliliter level. Furthermore, an “OR” logic gate was constructed for the simultaneous detection of dual mycotoxins, highlighting the platform's capability for multiplexed hazard monitoring. Overall, CBD demonstrates significant potential as a new paradigm for next-generation biosensing technologies.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118412"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LDOB: multi-dimensional programmable lactose-derived oligosaccharide biosensors","authors":"Li-Hua Liu ,&nbsp;Bo Xu ,&nbsp;Ying Huang ,&nbsp;Rongrong Chen ,&nbsp;Yunxia Li ,&nbsp;Yu Zhang ,&nbsp;Hong Huang ,&nbsp;Kuo Zhang ,&nbsp;Junyang Huang ,&nbsp;Ao Jiang","doi":"10.1016/j.bios.2026.118407","DOIUrl":"10.1016/j.bios.2026.118407","url":null,"abstract":"<div><div>Human milk oligosaccharides (HMOs) are the third most abundant solid constituent of breast milk and exert anti-infective, prebiotic and immunomodulatory functions. Microbial <em>de novo</em> synthesis is currently the only scalable route for commercial HMOs production, yet the absence of rapid and universal analytical tools has become a major bottleneck for strain improvement. Exploiting the strict stoichiometry between lactose consumption and HMOs biosynthesis, we constructed a set of <em>Escherichia coli</em>-based lactose-derived oligosaccharide biosensors (LDOB)—genetically engineered whole-cell systems that translate lactose concentration into inversely correlated biomass and fluorescence read-outs, enabling real-time screening of HMO-producing strains without downstream metabolite processing. The sensing circuit integrates multidimensional negative-feedback modules—encompassing multi-repression, targeted protein degradation and an occluded ribosome binding site (oRBS)—endowing LDOB with a wide dynamic range, straightforward operability, excellent compatibility, and substantial application potential. Validation experiments demonstrated that LDOB signals exhibited strong consistency with the yields of key HMOs, including 2′-fucosyllactose (2′-FL) and lacto-N-neotetraose (LNnT). Furthermore, coupling LDOB with fluorescence-activated droplet sorting (FADS) enabled high-throughput screening of evolved strains, resulting in remarkable titer enhancements of 42.8 % for 2′-FL and 86.4 % for LNnT, respectively. Collectively, these findings fully confirm the superiority of LDOB in monitoring HMOs synthesis and screening high-yield HMOs-producing strains, providing a valuable tool for advancing HMOs biomanufacturing.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118407"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145996868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoarchitectonics of bacteriophage-based click fluorescent biosensing platform for detection of Pseudomonas fluorescens","authors":"Heng Zhou ,&nbsp;Xiru Zhang ,&nbsp;Junyu Pan ,&nbsp;Xinna Xie ,&nbsp;Bo Qu ,&nbsp;Feng Zhao ,&nbsp;Yujun Jiang ,&nbsp;Xianlong Zhang","doi":"10.1016/j.bios.2026.118406","DOIUrl":"10.1016/j.bios.2026.118406","url":null,"abstract":"<div><div><em>Pseudomonas fluorescens</em> (<em>P. fluorescens</em>) is a typical psychrotrophic bacterium responsible for raw milk spoilage and potential human infections, highlighting the need for rapid and reliable detection. In this study, we developed a clickase-based fluorescent biosensing platform for the sensitive and specific detection of <em>P. fluorescens</em>. Herein, a novel copper(I)/Cys–RGD nanocatalyst (namely CCRN) with excellent CuAAC reaction catalytic activity was developed, and the prepared CCRN was functionalized with a <em>P. fluorescens</em>–targeting bacteriophage to form a specific recognition probe (CCRN@Phage). Remarkably, integrating the CCRN@Phage probe with ConA@Fe₃O₄ enabled magnetic enrichment and bioorthogonal catalytic signal amplification for sensitive and specific detection of <em>P. fluorescens</em> without external reducing agents. Specifically, <em>P. fluorescens</em> was first enriched using ConA@Fe₃O₄ and then captured by the CCRN@Phage probe to form a sandwich complex. Upon magnetic isolation, the complex catalyzed a copper(I)–catalyzed azide−alkyne cycloaddition (CuAAC) reaction between 3–azido–7–hydroxycoumarin (azide 1) and 3–butyn–1–ol (alkyne 2), generating a fluorescent triazole product. The CCRN catalyst exhibited excellent stability and catalytic activity, functioning efficiently without dissolution or external reducing agents. The developed method for detection of <em>P. fluorescens</em> had a limit of detection (LOD) of 1 CFU/mL in a linear response from 10² to 10⁷ CFU/mL. This strategy was successfully applied to food samples, showing a great potential for practical applications in food safety monitoring.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118406"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146008124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strategic aptamer evolution for SARS-CoV-2 to enable the detection in human saliva","authors":"Daimei Miura ,&nbsp;Wakana Hayashi ,&nbsp;Sakura Nashiki ,&nbsp;Katsuya Oguchi ,&nbsp;Haruka Kawai ,&nbsp;Kaori Tsukakoshi ,&nbsp;Wakako Tsugawa ,&nbsp;Ryutaro Asano ,&nbsp;Kazunori Ikebukuro","doi":"10.1016/j.bios.2026.118423","DOIUrl":"10.1016/j.bios.2026.118423","url":null,"abstract":"<div><div>In this study, we enhanced the affinity and specificity of an aptamer for SARS-CoV-2 detection in human saliva. Aptamers are promising recognition elements in biosensors; however, their performance in biological samples such as human saliva, is sometimes limited by insufficient affinity and specificity, even if they are obtained through conventional systematic evolution of ligands via exponential enrichment (SELEX). Here, we introduced inosine mutation into <em>in silico</em> maturation (ISM) process as a post-SELEX optimization. Inosine incorporation expands sequence diversity through non-Watson-Crick base pairing and increases local hydrophobicity at the aptamer-protein interface, enabling structural and physicochemical variation that cannot be achieved by SELEX alone. After inosine-incorporated ISM, we identified an optimized SARS-CoV-2 aptamer, WiS-914, which exhibited improved affinity and specificity for inactivated SARS-CoV-2. This aptamer showed a distinct and more stable topology than the original aptamer. When it was applied to inactivated SARS-CoV-2 detection in actual human saliva using our convenient and on-site virus detection platform, more sensitive detection with lower limit of detection was achieved compared with the original aptamer. These results demonstrate that inosine-integrated ISM is an effective post-SELEX optimization for generating high-performance aptamers suitable for detecting targets in biological samples.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118423"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotin-specific molecularly imprinted polymers as a biomimetic test line in lateral flow assays","authors":"Jennifer Marfà ,&nbsp;Anaixis del Valle ,&nbsp;Rafael Rovatti Pupin ,&nbsp;Bàrbara Baro ,&nbsp;Quique Bassat ,&nbsp;Maria Del Pilar Taboada Sotomayor ,&nbsp;María Isabel Pividori","doi":"10.1016/j.bios.2026.118415","DOIUrl":"10.1016/j.bios.2026.118415","url":null,"abstract":"<div><div>Lateral flow assays are widely used for rapid diagnostics at the point of care. However, their dependence on biological receptors limits their stability, scalability, and cost-effectiveness. This work demonstrates the use of biotin-specific molecularly imprinted polymers (biotin-MIPs) as synthetic recognition elements in nucleic acid lateral flow (NALF) assays. The biotin-MIPs were synthesized, structurally characterized, and integrated into the nitrocellulose membrane as a test line. Their selective binding affinity was first validated using biotinylated horseradish peroxidase as a model analyte. The platform was then evaluated for the detection of double-tagged PCR amplicons from <em>Escherichia coli</em> labeled with biotin and digoxigenin, achieving a visual detection limit of 2 ng mL⁻¹ and a limit of detection of 1.8 ng mL⁻¹, with no detectable signal in negative controls. Clinical feasibility was further assessed retrospectively using swab specimens collected during routine third-trimester screening (35–37 weeks of gestation) for Group B Streptococcus, a major cause of neonatal sepsis. In this proof-of-concept study, the MIP-based NALF assay showed complete qualitative agreement with the qPCR reference classification and the gold standard microbiological culture, demonstrating the compatibility of this approach with battery-operated portable PCR amplification. Unlike biological receptors, MIPs offer robustness, long-term stability at room temperature, and animal-free scalable production. These features position the MIP-based NALF platform as a cost-effective alternative to antibody-based tests and a promising foundation for next-generation lateral flow diagnostics for the detection of communicable diseases at the point of care.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118415"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146075045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HF-Sensor: A non-invasive biosensor system for heart failure diagnosis and monitoring at the point-of-care","authors":"Miguel A.D. Neves ,&nbsp;Andrea Cruz ,&nbsp;Pedro Melo ,&nbsp;Evelyn Santos ,&nbsp;Francisco Vasques-Nóvoa ,&nbsp;Ana Beatriz Baptista ,&nbsp;João Barbosa-Breda ,&nbsp;Fernando Friões ,&nbsp;Jorge Almeida ,&nbsp;João Pedro Ferreira ,&nbsp;Adelino Leite-Moreira ,&nbsp;Amândio Rocha-Sousa ,&nbsp;Inês Mendes Pinto","doi":"10.1016/j.bios.2026.118427","DOIUrl":"10.1016/j.bios.2026.118427","url":null,"abstract":"<div><div>Heart failure (HF) is a major public health challenge, contributing to high morbidity, mortality, and significant economic burden worldwide. Early diagnosis and accurate risk stratification are critical for improving patient outcomes and guiding clinical decisions. N-terminal pro–B-type natriuretic peptide (NT-proBNP) is a well-established blood biomarker of ventricular overload, widely used for diagnosing HF and assessing its severity. Beyond diagnosis, NT-proBNP provides valuable prognostic insights into treatment response and can predict adverse cardiovascular events, such as HF-related hospitalizations and mortality, thus playing a key role in personalized care and therapeutic management. Despite its clinical significance, current CE/FDA-certified methods for NT-proBNP measurement face critical limitations, particularly in point-of-care settings and for ongoing, minimally or non-invasive monitoring. These constraints hinder the ability to perform remote (e.g. community medical clinic) monitoring—a crucial capability for revolutionizing heart failure management. To address this challenge, we report the development and proof-of-concept validation of an ultrasensitive (compared to gold standard enzyme-linked immunosorbent assays), miniaturized electrochemical biosensor for fast and minute sample detection of NT-proBNP in tears from a well-characterized cohort of heart failure patients. Correlation analysis of NT-proBNP concentrations in tear fluid and blood plasma/serum, together with key clinical parameters, demonstrates that tear fluid is a promising non-invasive matrix for heart failure diagnosis and continuous monitoring in point-of-care settings. This approach provides a transformative pathway to advance heart failure management by enabling remote, point-of-care diagnostic monitoring outside of centralized hospitals and laboratories.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118427"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}