Biosensors and Bioelectronics最新文献_第7页

Diagnosis of prosthetic joint infection based on Cas12a-catalytic aggregation mediated by supramolecular chemistry 基于超分子化学介导的cas12a催化聚集诊断假体关节感染

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-20 DOI: 10.1016/j.bios.2025.118015

Yining Zhao , Ziye Liu , Ziting Liu , Juan Zhang , Jinghui Peng , Genxi Li

{"title":"Diagnosis of prosthetic joint infection based on Cas12a-catalytic aggregation mediated by supramolecular chemistry","authors":"Yining Zhao , Ziye Liu , Ziting Liu , Juan Zhang , Jinghui Peng , Genxi Li","doi":"10.1016/j.bios.2025.118015","DOIUrl":"10.1016/j.bios.2025.118015","url":null,"abstract":"<div><div>Prosthetic joint infection (PJI), which is still difficult to be diagnosing, is caused by bacterial infection. Rapid detection for trace amounts of bacteria should be a solution; however, the existing assays cannot serve for PJI diagnosis due to the inherent limitations. Herein, a strategy based on supramolecular chemistry mediated Cas12a catalytic aggregation (CCA) is explored in this work for ultrasensitive detection of universal bacteria. In the design, supramolecular chemistry is introduced to impede the cis-cleavage of activated Cas12a, resulting in its aggregation in the surrounding of bacteria with the linkage of CCA probe. Since Cas12a aggregation owns excellently enhanced catalytic efficiency, the CCA-based strategy can be used for bacterial analysis with a limit of detection as low as 1 CFU/mL. With high sensitivity, reproducibility, generalizability, and stability, the CCA-based strategy can be applied for the detection of clinical effusion samples. The remarkable performance of CCA-based strategy has also demonstrated its robust potential to be a simple, rapid, and highly sensitive bacteria diagnostic tool, paving the way for prompt diagnosis and management of PJI.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118015"},"PeriodicalIF":10.5,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145120715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A ratiometric fluorescent paper–based sensor based on Fe/Eu-MOF for visual and sensitive detection of diethylstilbestrol 基于Fe/Eu-MOF的比例荧光纸型传感器用于二乙基己烯雌酚的视觉灵敏检测

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-20 DOI: 10.1016/j.bios.2025.118017

Dongmei Liu , Xinhui Jiang , Xiaoming Li , Hecun Zou , Lin He , Zhengwei Zhang , Siqiao Li , Xintong Bian

{"title":"A ratiometric fluorescent paper–based sensor based on Fe/Eu-MOF for visual and sensitive detection of diethylstilbestrol","authors":"Dongmei Liu , Xinhui Jiang , Xiaoming Li , Hecun Zou , Lin He , Zhengwei Zhang , Siqiao Li , Xintong Bian","doi":"10.1016/j.bios.2025.118017","DOIUrl":"10.1016/j.bios.2025.118017","url":null,"abstract":"<div><div>Diethylstilbestrol (DES), a synthetic estrogen, is associated with severe endocrine disruption and carcinogenic risks, posing persistent biological and environmental hazards. Herein, a bimetallic Fe/Eu–based metal–organic framework (Fe/Eu–MOF) with distinct multi–emission properties was developed as a ratiometric fluorescent sensor for sensitive and intelligent visual detection of DES. The Fe/Eu–MOF exhibited stable red emission at 615 nm and DES–responsive blue emission at 410 nm, enabling self–referenced quantification. The sensor showed two linear ranges (0.005–2 and 2–30 μg/mL), a low detection limit (1.78 ng/mL), and excellent selectivity against potential interferents. Its practicality was validated in milk and shrimp, demonstrating excellent recovery and reproducibility. Additionally, a paper–based sensor integrated with smartphone RGB analysis enabled rapid, on–site, and instrument–free detection. Mechanism studies revealed that DES coordination with Fe centers suppressed non–radiative decay and enhanced fluorescence. This work provides a robust and intelligent platform for portable monitoring of DES residues, offering significant potential for real–time biohazard surveillance and visual diagnostics in resource–limited settings.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118017"},"PeriodicalIF":10.5,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145120425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic immune-network-oriented probe based on pCuGA-AFcI for enhanced on-site detection of pentachlorophenol 基于pCuGA-AFcI的协同免疫网络探针增强五氯酚的现场检测

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-19 DOI: 10.1016/j.bios.2025.118012

Feier Bai , Hui Zhang , Tong Bu , Chenxi Gao , Yanmin Liang , Mingyan Li , Zhanhui Wang , Bing Shao

{"title":"Synergistic immune-network-oriented probe based on pCuGA-AFcI for enhanced on-site detection of pentachlorophenol","authors":"Feier Bai , Hui Zhang , Tong Bu , Chenxi Gao , Yanmin Liang , Mingyan Li , Zhanhui Wang , Bing Shao","doi":"10.1016/j.bios.2025.118012","DOIUrl":"10.1016/j.bios.2025.118012","url":null,"abstract":"<div><div>Orientation-specific immobilization of antibodies (Abs) onto nanoparticles to maintain Abs-antigen recognition is one of the main strategies to improve immunochromatographic assay (ICA) performance. Inspired by an immune-network-based probe synthesis strategy, we proposed a novel immune-bridging mechanism utilizing the specific interaction between anti-Fc immunoglobulins (AFcI) and Abs. This enhanced affinity facilitates the construction of immune-network, thereby improving the stability of Abs binding. Herein, a bio-derived tracer, pupal-like CuGA (pCuGA), self-assembled from Cu<sup>2+</sup> and gallic acid (GA) was developed and further modified with AFcI. This modification addresses potential Abs shedding caused by unstable carrier-Abs binding and enables synergistic Abs orientation for immune-network stabilization and signal amplification. The resulting tracer exhibits excellent biocompatibility and preserves Abs bioactivity through a simple stirring-based labeling process, eliminating the need for cumbersome chemical modification. Compared with the conventional pCuGA-Ab probe-based ICA, the pCuGA-AFcIAb probe-based ICA not only improved sensitivity by halving Ab consumption but also achieved significantly lower detection limits, with an IC<sub>10</sub> of 0.019 ng/mL and a cut-off value of 3.00 ng/mL, representing at least 3.2- and 4.0-fold enhancements, respectively. Furthermore, the feasibility of the method was verified in chopsticks, fish, environmental water, fetal bovine serum, and shrimp paste samples, yielding satisfactory recoveries. The results of blind samples detection were consistent with those of liquid chromatography tandem mass spectrometry. This study provides new possibilities for the functional modification of ICA tracers and paves the green road for ICA detection.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 118012"},"PeriodicalIF":10.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145096322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-enhanced biohybrid frameworks as novel ECL emitters for sensitive detection of CA15-3 自增强生物混合框架作为新型ECL发射体用于CA15-3的灵敏检测

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-19 DOI: 10.1016/j.bios.2025.118013

Lipeng Liu , Wenze Chen , Lu Yin , Xiaojun Sun , Yan Li , Qin Wei , Hongmin Ma

{"title":"Self-enhanced biohybrid frameworks as novel ECL emitters for sensitive detection of CA15-3","authors":"Lipeng Liu , Wenze Chen , Lu Yin , Xiaojun Sun , Yan Li , Qin Wei , Hongmin Ma","doi":"10.1016/j.bios.2025.118013","DOIUrl":"10.1016/j.bios.2025.118013","url":null,"abstract":"<div><div>Early screening for breast cancer can be effective in reducing cancer mortality; therefore, accurate detection of breast cancer marker carbohydrate antigen 15–3 (CA15-3) is of great importance. In this study, a highly sensitive sandwich-type electrochemiluminescence (ECL) biosensor based on an electron transfer quenching mechanism was developed for the ultrasensitive detection of the CA15-3. The self-assembled biohybrid framework through hydrogen bonding between the amino groups (-NH<sub>2</sub>) of bovine serum albumin (BSA) and the carboxylic acid groups (-COOH) of perylene tetracarboxylic acid (PTCA) was used as a luminophore. The aggregation-caused quenching (ACQ) effect was effectively suppressed by framework spatial arrangement preventing PTCA aggregation and enhancing ECL emission. Simultaneously, introduction of Au NCs with BSA as a protective ligand catalyzed the production of more SO<sub>4</sub><sup>•−</sup> radicals from S<sub>2</sub>O<sub>8</sub><sup>2−</sup> to enhance ECL emission intensity. Cu<sub>2</sub>O@MnO<sub>2</sub> with matched energy level can be used as an electron transfer quencher to construct sandwich-type ECL biosensors. ECL signals was quenched by target response via the electron transfer (ET) pathway. The sensor achieves sensitive detection of CA15-3 under optimal conditions with a wide response range (0.001–100 U/mL) and a low limit of detection (LOD) (0.00014 U/mL), and has sufficient practical analytical performance. This study provides a high-precision analytical platform for the sensitive detection of CA15-3 and offers more possibilities for the development of ECL systems based on novel biohybridisation frameworks.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 118013"},"PeriodicalIF":10.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SiNWs-DNA nanoprobes functionalized microchannel for rapid and ultrasensitive virus sensing 用于快速和超灵敏病毒检测的SiNWs-DNA纳米探针功能化微通道

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-19 DOI: 10.1016/j.bios.2025.118010

Yueyue Feng , Duo Zhang , Shiwei Xu , Guofeng Wang , Liying Huang , Yajun Wang , Nan Li , Pengpeng Xiao , Nannan Liu

引用次数: 0

From tongue to eye: A biomimetic colorimetric sensor for umami substances based on taste receptor and plasmonic gold nanoparticles 从舌头到眼睛：一种基于味觉感受器和等离子体金纳米粒子的鲜味物质仿生比色传感器

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-18 DOI: 10.1016/j.bios.2025.118007

Xihang Xu , Ruijie Fu , Yidan Wang , Xiaoxue Zhu , Yunlei Xianyu

{"title":"From tongue to eye: A biomimetic colorimetric sensor for umami substances based on taste receptor and plasmonic gold nanoparticles","authors":"Xihang Xu , Ruijie Fu , Yidan Wang , Xiaoxue Zhu , Yunlei Xianyu","doi":"10.1016/j.bios.2025.118007","DOIUrl":"10.1016/j.bios.2025.118007","url":null,"abstract":"<div><div>Taste perception plays a crucial role in human survival and food quality assessment. Traditional methods for detecting taste substances suffer from limitations such as complex instrumental analysis and subjective evaluation, highlighting the need for developing simple and quantitative methods. Biomimetic sensors that mimic biological sensation to achieve taste evaluation while ensuring objective quantification and high sensitivity have emerged as a promising strategy. In this study, we designed a biomimetic colorimetric sensor based on plasmonic gold nanoparticles (AuNPs) and taste receptors. The Venus Flytrap domain (VFT) of the umami receptor T1R1, expressed heterologously in <em>Escherichia coli</em>, served as the recognition element to specifically recognize the umami substances. Hydrogen bonding interactions between the receptor and umami substances affected the charge balance on the surface of AuNPs, and the localized surface plasmon resonance (LSPR) properties of AuNPs enabled the quantitative detection with visible readout. Through the surface growth of AuNPs, we modulated the spacing between the receptor and AuNPs, thereby amplifying the signal for highly sensitive detection of sodium glutamate (MSG) with high selectivity and a detection limit of 0.0293 mM. Molecular docking results revealed multiple key binding sites between MSG and T1R1-VFT, contributing to its high affinity for the receptor. This biomimetic sensor was applied to detection in real food samples, demonstrating good specificity and stability. This study provides a promising approach for taste detection that holds great potential in food quality inspection and monitoring of food freshness.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118007"},"PeriodicalIF":10.5,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combining nanobody generation platform, 3D-printed microfluidic chip, and smartphone detection system for monitoring emerging virus-caused diseases 结合纳米体生成平台、3d打印微流控芯片和智能手机检测系统，监测新发病毒引起的疾病

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-18 DOI: 10.1016/j.bios.2025.117972

Yanhong Ji , Fengyun Li , Zhaowei Qu , Xiangkun Wang , Pengfei Cui , Guohua Deng , Shaorong Liu , Qiaosheng Pu , Qiyun Zhu , Apeng Chen

{"title":"Combining nanobody generation platform, 3D-printed microfluidic chip, and smartphone detection system for monitoring emerging virus-caused diseases","authors":"Yanhong Ji , Fengyun Li , Zhaowei Qu , Xiangkun Wang , Pengfei Cui , Guohua Deng , Shaorong Liu , Qiaosheng Pu , Qiyun Zhu , Apeng Chen","doi":"10.1016/j.bios.2025.117972","DOIUrl":"10.1016/j.bios.2025.117972","url":null,"abstract":"<div><div>Rapid in-field detection of emerging viruses is crucial for preventing potential pandemics. This study presents the development of a portable, cost-effective point-of-care testing (POCT) device for detecting emerging pathogens. We took H7N9 avian influenza virus (AIV) as an example, utilizing a combination of novel nanobodies, 3D-printed microfluidic chips, and smartphones to monitor the pathogen. The system employs projection micro-stereolithography (PμSL) 3D printing to fabricate a flower-shaped microfluidic chip, which integrates a micropillar array to enhance the surface area for nanobody immobilization. A pair of nanobodies with high specificity and affinity for H7N9 was generated to develop a sensitive colorimetric immunoassay. The assay results can be visually inspected and further quantified using smartphone imaging, eliminating the need for external lighting devices. The device demonstrated a limit of detection (LOD) of 5.9 × 10<sup>3</sup> EID<sub>50</sub>/0.1 mL, comparable to traditional ELISA methods, and was validated on real-world samples. The microfluidic chip's reusability was also established, with up to nine reuse cycles without significant loss of sensitivity. The smartphone-integrated POCT device offers the advantages of portability, simplicity, and rapidity, making it a promising tool for in-field H7N9 virus screening, especially in resource-limited settings. This approach exemplifies the potential of integrating advanced nanotechnology with modern manufacturing techniques and ubiquitous devices to enhance diagnostic accessibility and efficiency.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 117972"},"PeriodicalIF":10.5,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145096319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A homogeneous electrochemical aptasensor based on Pd@rGO and targeted methylene blue enrichment for sensitive detection of dibutyl phthalate 基于Pd@rGO和靶向亚甲基蓝富集的均相电化学感应传感器用于邻苯二甲酸二丁酯的灵敏检测

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-18 DOI: 10.1016/j.bios.2025.118009

Xinyao Li , Zhan Diao , Jiangbo Dong, Xiaofang Liu, Xiaogang Luo, Changjun Hou, Danqun Huo

{"title":"A homogeneous electrochemical aptasensor based on Pd@rGO and targeted methylene blue enrichment for sensitive detection of dibutyl phthalate","authors":"Xinyao Li , Zhan Diao , Jiangbo Dong, Xiaofang Liu, Xiaogang Luo, Changjun Hou, Danqun Huo","doi":"10.1016/j.bios.2025.118009","DOIUrl":"10.1016/j.bios.2025.118009","url":null,"abstract":"<div><div>Efficient detection of phthalate esters (PAEs) is of great significance for assessing ecological environment and food safety. This work developed an electrochemical aptasensor based on a Pd@rGO/GCE sensing electrode, which utilizes target-induced conformational changes of G-quadruplex (G4) to regulate the accumulation of methylene blue (MB) on the electrode surface for homogeneous and efficient detection of dibutyl phthalate (DBP). Benefiting from the target-triggered competitive release of truncated strands (H1 and H2) in homogeneous solution, the efficient adsorption of MB by G4, and the excellent conductivity and electrochemical activity of Pd@rGO/GCE, the constructed biosensor achieved a detection limit down to 9.3 × 10<sup>−7</sup> mg/mL. It also exhibited high specificity toward PAEs, effectively distinguishing interfering substances, and demonstrated remarkable reproducibility and stability. Material characterization (SEM, EDS, XRD and XPS) confirmed the formation and uniform distribution of Pd NPs on the rGO surface, ensuring high electrochemical activity. More importantly, the homogeneous solution-based detection is free from interference related to electrode surface materials, charge, or steric hindrance, making the detection more accurate and efficient. Furthermore, experimental investigations demonstrated the structural advantages and signal amplification capability of the truncated strands. Overall, this work provides a novel, efficient, and reliable method for PAEs detection, offering valuable insights for applications in environmental monitoring and food safety.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118009"},"PeriodicalIF":10.5,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145218370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A dual-mode portable biosensor based on photothermal-pyroelectric-electrochromic effects for versatile detection of enrofloxacin 基于光热-热释电-电致变色效应的便携式双模生物传感器用于恩诺沙星的多功能检测

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-18 DOI: 10.1016/j.bios.2025.118006

Jiajie Zhang , Yuanzhen Ning , Guifen Jie , Ziao Zong , Fu Chai

{"title":"A dual-mode portable biosensor based on photothermal-pyroelectric-electrochromic effects for versatile detection of enrofloxacin","authors":"Jiajie Zhang , Yuanzhen Ning , Guifen Jie , Ziao Zong , Fu Chai","doi":"10.1016/j.bios.2025.118006","DOIUrl":"10.1016/j.bios.2025.118006","url":null,"abstract":"<div><div>Traditional photoelectrochemical (PEC) sensors are limited by bulky electrochemical workstations and high-energy light sources, thus limiting their practical applications. In this study, the photothermal effect of iron single-atom materials (Fe-PNC), pyroelectric effect of Yb-doped Bi<sub>2</sub>S<sub>3</sub> (Yb-Bi<sub>2</sub>S<sub>3</sub>), and electrochromic effect of polyaniline (PANI) were innovatively combined to construct a dual-signal portable PEC sensing platform without the need of workstation. Specifically, we designed ITO electrode region: Yb-Bi<sub>2</sub>S<sub>3</sub> nanorods were modified in the photothermal region, and PANI was electrodeposited in the discolored region. The Fe-PNC was introduced into the photothermal region through target-aptamer (Apt) sandwich strategy. Under the irradiation of 808 nm light, Fe-PNC converts light energy into heat energy, causing the temperature in the photothermal region to rise rapidly, thereby enhancing the pyroelectric effect of Yb-Bi<sub>2</sub>S<sub>3</sub> and promoting carrier separation, so a large number of generated pyroelectric electrons migrate to the discolored region, and they trigger the PANI's reduction color-changing reaction (blue to green), thus achieving dual signal output of temperature and color. Based on this, we have successfully achieved highly sensitive and selective detection of enrofloxacin (ENR), which has the advantages of rapid response and intuitive reading. This design not only overcomes the problem of insufficient photogenerated carriers caused by the low energy of near-infrared (NIR) light, but more importantly, uses temperature and color changes to replace the traditional current signal detection, enabling the PEC sensor free of the electrochemical workstation. This research provides new ideas for the development of portable and low-cost PEC sensors.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 118006"},"PeriodicalIF":10.5,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145096321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced intracellular recording in cardiomyocytes via soft ionic–electronic conductive interfaces of PEDOT: PSS-modified electrodes 通过PEDOT: pss修饰电极的软离子-电子导电界面增强心肌细胞内记录

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-18 DOI: 10.1016/j.bios.2025.117999

Duote Cai , Qunchen Yuan , Peng Sun , Haitao Liu , Qianwen Xiong , Tao Liang , Li Niu , Weiwei Chen , Yi Jin , Shuhao Zhang , Ning Hu , Chunlian Qin , Zhigang Gao

{"title":"Enhanced intracellular recording in cardiomyocytes via soft ionic–electronic conductive interfaces of PEDOT: PSS-modified electrodes","authors":"Duote Cai , Qunchen Yuan , Peng Sun , Haitao Liu , Qianwen Xiong , Tao Liang , Li Niu , Weiwei Chen , Yi Jin , Shuhao Zhang , Ning Hu , Chunlian Qin , Zhigang Gao","doi":"10.1016/j.bios.2025.117999","DOIUrl":"10.1016/j.bios.2025.117999","url":null,"abstract":"<div><div>Precise recording and stimulation of cardiomyocyte electrical activity with high spatiotemporal resolution is critical for understanding cardiac electrophysiology and associated pathologies. Here, we present a high-performance soft conductive polymer interface based on poly (3,4-ethylenedioxythiophene): polystyrene sulfonate (PEDOT: PSS)-modified electrodes for reliable intracellular electrophysiological recording. The electrodes are fabricated using standard microfabrication followed by electrochemical electropolymerization of PEDOT: PSS, a conductive polymer known for its mixed ionic-electronic conductivity, biocompatibility, and mechanical softness. Compared to bare Au electrodes, PEDOT: PSS-modified electrodes exhibit significantly reduced impedance, enhanced charge storage capacity, and improved electrode–cell coupling. Primary cardiomyocytes cultured on the modified surfaces show robust adhesion and high viability. Using a self-developed biosensing-regulating electrophysiological system, PEDOT: PSS-modified electrodes enable repeated intracellular action potential recordings with high amplitude, high signal-to-noise ratio, and stable waveforms across multiple electroporation cycles and over 5 days. These findings demonstrate the potential of PEDOT: PSS-modified electrodes as minimally invasive, high-fidelity bioelectronic interfaces for cardiac electrophysiological research, high-throughput drug screening, and future implantable biomedical applications.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 117999"},"PeriodicalIF":10.5,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145096314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0