Biosensors and Bioelectronics最新文献

{"title":"An ultrasensitive FEN1-aided LCR-electrochemical biosensor enables detection of BCR/ABL1 fusion transcripts in clinical samples for early diagnosis and minimal residual monitoring of CML","authors":"Liang-Yong Yang ,&nbsp;Guang-Hui Mei ,&nbsp;Xin-Ru Lin ,&nbsp;Cai-Hong Wang ,&nbsp;Rui-Long Lan ,&nbsp;Chen-Liu Ye ,&nbsp;Yan-Fang Xu ,&nbsp;Xin-Hua Lin ,&nbsp;Jin-Yuan Chen","doi":"10.1016/j.bios.2026.118442","DOIUrl":"10.1016/j.bios.2026.118442","url":null,"abstract":"<div><div>Ligase chain reaction (LCR) is a promising nucleic acid amplification technique widely applied in molecular diagnosis. However, a critical limitation of LCR is target-independent amplification, primarily caused by the blunt-end ligation activity of thermophilic DNA ligases, which results in false positives and compromises sensitivity. To overcome this challenge, we developed a thermostable flap endonuclease 1 (FEN1)-aided LCR (FALCR) employing DNA probes with non-phosphorylated sticky ends. In each amplification cycle, FEN1 selectively cleaves the 5′ DNA flap of the downstream probe in the presence of a DNA template, generating 5′-phosphorylated DNA nicks that enable ligation. In the absence of a DNA template, the non-phosphorylated sticky ends of the probes prevent ligation, effectively eliminating nonspecific amplification. We further integrated FALCR with a magnetic platform-based electrochemical biosensor (FA-eLCR), achieving ultrasensitive detection of DNA targets with a limit of detection (LOD) of 0.1 aM and a dynamic range spanning 10 aM to 10 fM. Furthermore, the designing of a duplex FALCR enabled the detection of BCR/ABL1^p210 comprising e13a2/e14a2/both isoforms in a single assay. Finally, the duplex FA-eLCR successfully detected the BCR/ABL1^p210 transcripts in patients with chronic myeloid leukemia (CML) and monitored the change of transcripts during treatment, with 100 % concordance to reverse transcription-quantitative polymerase chain reaction, highlighting its high potential in the early diagnosis and minimal residual disease (MRD) monitoring of CML. This work presents a novel strategy to address nonspecific amplification in LCR and introduces a cost-effective, highly sensitive platform for molecular diagnosis in clinical setting.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"299 ","pages":"Article 118442"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146076604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel self-powered electrochemical sensor based on zinc-air battery for thrombin detection","authors":"Zhengchun Sun ,&nbsp;Weili Zhang ,&nbsp;Junhong Liu ,&nbsp;Shina Du ,&nbsp;Yuebo Wang ,&nbsp;Zhongfang Li ,&nbsp;Xueliang Niu ,&nbsp;Wei Sun","doi":"10.1016/j.bios.2026.118429","DOIUrl":"10.1016/j.bios.2026.118429","url":null,"abstract":"<div><div>As an emerging sensing mode, self-powered electrochemical sensor (SPES) has gained much attention for its advantages of without external power supply and portable detection. However, developing SPES with enhanced output signals and anti-influence characteristics is still a challenge. To address these issues, herein, a novel zinc-air battery (ZAB)-based SPES with aptamer as a specific recognition element was constructed. In this case, bimetallic Fe/Ni and N co-doping carbon and in-situ grown carbon nanotubes (Fe-Ni/N-C/CNT) was synthesized by mixing Fe, Ni precursors with previously prepared ZIF-8. Under neutral conditions, Fe-Ni/N-C/CNT exhibits a high half-wave potential of 0.831 V (<em>vs</em>. RHE) and the ZAB assembled with Fe-Ni/N-C/CNT as an air cathode generates a considerable maximum peak power density (<em>P</em>_max) of 84.1 mW cm⁻². As a concept, thrombin (TB) was selected as a detection target, a “signal-off” SPES based on the interfacial steric hindrance effect generated by aptamers structure bending while capturing the target was constructed with <em>P</em>_max as a sensing signal. <em>P</em>_max values of the prepared ZAB-based SPES show a good linear relationship with logarithm of TB concentrations, accompanied by a linear range from 2 × 10⁻⁴ nM to 2 × 10⁻² nM, and a detection limit of 66.7 fM (LOD). The constructed ZAB-based SPES shows good anti-interference ability, stability, and reproducibility, making it feasible and efficient in practical applications. Furthermore, this SPES strategy displays a promising generalizability for other targets detection by replacing the aptamers modified on the sensing cathode surface.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"299 ","pages":"Article 118429"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146076605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual CRISPR/Cas-driven amplification-free surface-enhanced Raman scattering biosensor combined with a smartphone for simultaneous detection of total and live target bacteria","authors":"Ruibao Jiao ,&nbsp;Qun Ni ,&nbsp;Ruirui Zhao ,&nbsp;Xiaofan Zhu ,&nbsp;Lixia Wang ,&nbsp;Jingran Jiao ,&nbsp;Han Jiang ,&nbsp;Qian Wu ,&nbsp;Shuang Yao ,&nbsp;Li Yao ,&nbsp;Kaiyong Liu ,&nbsp;Panzhu Qin","doi":"10.1016/j.bios.2026.118446","DOIUrl":"10.1016/j.bios.2026.118446","url":null,"abstract":"<div><div>Simultaneous detection of total and live counts of target bacteria is significant but challenging. To address this challenge, here we proposed a dual CRISPR/Cas-driven amplification-free surface-enhanced Raman scattering (SERS) biosensor, termed cc-SERS. The biosensor was constructed based on the property that DNA remains stable while some RNA degrades rapidly after bacterial death. Briefly, the target DNA and RNA from live bacteria could activate both CRISPR/Cas12a and CRISPR/Cas13a, while dead bacteria could only activate CRISPR/Cas12a through the target DNA. In the absence of the target bacteria, neither CRISPR/Cas12a nor CRISPR/Cas13a could be activated. Therefore, the characteristic Raman signal at 1079 cm⁻¹ generated by the target DNA-activated CRISPR/Cas12a indicated the presence of the target bacteria (the sum of dead and live), while the characteristic Raman signal at 593 cm⁻¹ produced by the target RNA-activated CRISPR/Cas13a indicated the presence of the live target bacteria. With this unique signaling pattern, the biosensor is capable of detecting both total and live target bacteria in a single tube with a detection limit of ∼10 CFU/mL. The introduction of a rapid pre-processing procedure and a smartphone-assisted portable Raman spectrometer enabled the entire process to be completed in the field within 45 min. Thanks to the excellent programmability of CRISPR/Cas systems, the biosensor has been successfully applied to the detection of <em>Staphylococcus aureus</em> and <em>Cronobacter sakazakii</em>, respectively. As a proof-of-concept, this work opens a promising avenue for the simultaneous detection of total and live target bacteria.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"299 ","pages":"Article 118446"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146076607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photoelectrochemical and colorimetric dual-mode immunosensor based on Cu-BTC-stabilized hemin nanozyme for point-of-care detection of neuronal-specific enolase in serum","authors":"Ke Xiao ,&nbsp;Sitong Huang ,&nbsp;Jia Sun ,&nbsp;Zhen Liu ,&nbsp;Leixia Meng","doi":"10.1016/j.bios.2026.118439","DOIUrl":"10.1016/j.bios.2026.118439","url":null,"abstract":"<div><div>As a primary cause of global mortality, cerebral stroke (CS) necessitates prompt diagnosis, where detecting neuron-specific enolase (NSE) rapidly and accurately is key to effective intervention. To resolve this issue, a novel dual-mode photoelectrochemical and colorimetric (PEC-CL) immunosensor was proposed for point-of-care detection of NSE. The sensing platform utilizes magnetic Fe₃O₄@Bi₂S₃ nanoparticles (NPs) functionalized with primary antibody as PEC-active capture probes and hemin-encapsulated Cu-based metal-organic framework (Cu-BTC@hemin) conjugated with secondary antibody as multi-functional signal probes. A specific dual-signal transduction is triggered by the formation of the sandwich immunocomplex upon NSE binding. This process involves electron transfer from hemin to Bi₂S₃, leading to enhanced attenuation of the PEC signal, and CL response from hemin of supernatant mediated catalytic of 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation. This sensor demonstrates outstanding performance and inherent self-verification capabilities. Furthermore, the dual-mode sensor displays robust consistency in NSE quantification across clinical samples. Its demonstrated excellence in specificity, reproducibility, and robustness in complex serum matrices establishes a solid groundwork for next-generation point-of-care CS diagnostics. Moreover, it offers a generalizable blueprint for creating multimodal biosensors for other biomarkers, thereby accelerating progress accelerating progress in precision medicine for cerebrovascular diseases.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"299 ","pages":"Article 118439"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146049222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition effect-involved colorimetric sensor array based on Ln-Ru dual-site nanozymes for thiol discrimination","authors":"Jiajun Li ,&nbsp;Lei Jiao ,&nbsp;Lu Dong ,&nbsp;Lijun Hu ,&nbsp;Xiangkun Jia ,&nbsp;Chengjie Chen ,&nbsp;Peipei Zong ,&nbsp;Yiming Zhang ,&nbsp;Xiaolei Sun ,&nbsp;Yanling Zhai ,&nbsp;Xiaoquan Lu","doi":"10.1016/j.bios.2026.118435","DOIUrl":"10.1016/j.bios.2026.118435","url":null,"abstract":"<div><div>The inhibitory interaction between nanozymes and thiols has been extensively investigated and developed for the establishment of thiol sensors. However, owing to the structural similarities of various thiols, the accurate distinction of thiols still remains a great challenge. Herein, a series of lanthanide-doped RuO₂ nanozymes (Ln-RuO_x) featuring Ln-Ru dual sites are developed and demonstrate enhanced peroxidase-like activity. Inhibition kinetics demonstrate that thiols induce a mixed inhibition mode on the Ln-RuO_x nanozymes, in contrast to the competitive mode on the RuO₂ nanozymes. Besides, by regulating the Ln elements in Ln-RuO_x nanozymes, the inhibitory interaction between Ln-RuO_x nanozymes and thiols is significantly changed, as evidenced by the inhibition constant, thus achieving the accurate distinction of various thiols. Finally, based on the differential inhibitory effects, a multichannel sensor array is established to discriminate six thiols with a detection limit of 1 μM. More importantly, this colorimetric sensor array discriminates between normal and cancer cells based on endogenous thiols, demonstrating its powerful application prospects in disease diagnosis.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"299 ","pages":"Article 118435"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146099590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecularly engineered π-electron relay enables coreactant-free, oxygen-driven electrochemiluminescence","authors":"Xijie Li ,&nbsp;Huayue Sun ,&nbsp;Li-Ping Zong ,&nbsp;Peng-Wen Xie ,&nbsp;Serge Cosnier ,&nbsp;Xue-Ji Zhang ,&nbsp;Dan Shan","doi":"10.1016/j.bios.2026.118464","DOIUrl":"10.1016/j.bios.2026.118464","url":null,"abstract":"<div><div>Luminol remains a foundational electrochemiluminescence (ECL) emitter, yet its performance is intrinsically restricted by slow electron transfer and the short-lived, diffusive nature of oxygen-derived intermediates. These limitations necessitate external coreactants or catalytic additives, obscuring mechanistic interpretation and reducing operational robustness. Here we demonstrate an unimolecular strategy to reorganize oxygen-driven ECL by covalently integrating phenanthroline and luminol through an imine linkage. The resulting π-extended scaffold forms an internal electron-relay pathway that enhances charge transport, promotes oxygen activation and stabilizes the radical intermediates required for light emission. The hybrid produces strong, coreactant-free ECL under air-saturated conditions, with mechanistic studies confirming a coordinated sequence of intramolecular electron flow, superoxide formation and excited-state generation within a confined redox microdomain. As a proof of concept, the emitter enables sensitive and selective detection of glutathione across a wide concentration range (0.5–1000 μM, detection limit 0.479 μM), with predictable quenching behavior that reflects the hybrid's reorganized oxygen-activation pathway. These findings establish a mechanism-guided molecular blueprint for designing next-generation, oxygen-responsive luminophores in which electronic coupling and structural confinement jointly dictate ECL efficiency.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"299 ","pages":"Article 118464"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146103397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic-induced autocatalytic DNA circuit for enrofloxacin detection based on triple signal amplification strategy","authors":"Junhua Chen ,&nbsp;Gu Shi ,&nbsp;Chong Yan","doi":"10.1016/j.bios.2026.118448","DOIUrl":"10.1016/j.bios.2026.118448","url":null,"abstract":"<div><div>Using hairpin DNA as the sensing probes and aptamer sequence as the recognition probe, we have successfully constructed an autocatalytic DNA circuit biosensor for enrofloxacin (ENR) detection with high sensitivity and selectivity. The antibiotic-induced release of the trigger DNA (T) can initiate the cross-hybridization of hairpin probes to form three-way DNA junction products, in which the originally split T fragments in hairpin probes can be pulled together to form the intact T sequence. Both the released T and the newly regenerated T can be reused to speed up the generation of the three-way DNA junction. In the products, intact Mg²⁺-dependent DNAzyme sequences will be generated at two arms of the DNA junctions, which can cleave the FAM and BHQ labeled substrate hairpin probe, leading to the release of another T. Through the triple signal amplification strategy in the autocatalytic DNA circuit, we can get an exponentially amplified signal for ENR detection. The DNA circuit biosensor is ultrasensitive with a detection limit of 25.8 fM. The sensing system is robust and has been applied to the detection of ENR in real fish and water samples with good reliability and accuracy. With the advantages of enzyme-free format, highly efficient signal amplification efficiency, and good robustness in complex samples, we anticipate that this biosensor will be a powerful tool for antibiotic residue monitoring in environmental and food samples.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"299 ","pages":"Article 118448"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146103349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic coupling of a spherical nucleic acid DNA walker and a redox cascade for self-calibrated ratiometric detection of endocrine disruptors","authors":"Na Wu ,&nbsp;Yijie Chen ,&nbsp;Lu Shi ,&nbsp;Weiheng Liu ,&nbsp;Jianping Xu ,&nbsp;Chenqi Niu ,&nbsp;Huaping Peng","doi":"10.1016/j.bios.2026.118372","DOIUrl":"10.1016/j.bios.2026.118372","url":null,"abstract":"<div><div>Endocrine-disrupting chemicals (EDCs), such as 17β-estradiol (E2) and bisphenol A (BPA), are pervasive environmental contaminants that pose significant health risks even at trace concentrations. Their on-site monitoring remains challenging due to insufficient sensitivity and stability of conventional detection methods. To address this, we developed a universal ratiometric fluorescence biosensor by integrating a target-activated spherical nucleic acid (SNA) with an enzyme-nanozyme- mediated redox cascade for the ultrasensitive and self-calibrating detection of EDCs. The sensing mechanism is initiated by aptamer recognition, which triggers a Nb. BbvCI-powered DNA walking circuit on gold nanoparticles (AuNPs), resulting in the release of numerous alkaline phosphatase (ALP) molecules. These ALP then catalyze the conversion of ascorbic acid 2-phosphate (AAP) to ascorbic acid (AA), which subsequently reduces CoOOH nanoflakes (CoOOH NFs) in a g-C₃N₄/CoOOH nanohybrid. This redox cascade quenches the oxidase-mimicking activity of CoOOH NFs, suppresses oxidation-mediated emission at 556 nm, and simultaneously restores the intrinsic fluorescence of g-C₃N₄ nanosheets at 448 nm. This anti-correlated dual-signal output enables accurate and stable quantification against environmental interference. The biosensor achieved detection limits of 100 pM for E2 and 240 pM for BPA, outperforming conventional colorimetric methods by 2 orders of magnitude. Furthermore, the practical applicability was successfully validated using real water samples yielding high recovery rates and demonstrating excellent reliability. By synergizing SNA amplification with an enzyme-nanozyme-modulated ratiometric sensing strategy, this work provides a robust and generalizable platform for the on-site screening of trace-level EDCs, holding great promise for applications in environmental monitoring and public health diagnostics.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"299 ","pages":"Article 118372"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146049223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Point-of-care profiling of H. pylori virulence and antibiotic resistance from endoscopic biopsies using an integrated restriction enzyme–CRISPR microfluidic platform","authors":"Lei Xu ,&nbsp;Jingzheng Jin ,&nbsp;Weiyuan Lyu ,&nbsp;Xiao Liang ,&nbsp;Qi Wang ,&nbsp;Jiajie Zhang ,&nbsp;Yang Luo ,&nbsp;Jinnan Chen ,&nbsp;Hong Lu ,&nbsp;Xiaobo Li ,&nbsp;Feng Shen","doi":"10.1016/j.bios.2026.118438","DOIUrl":"10.1016/j.bios.2026.118438","url":null,"abstract":"<div><div>Endoscopically obtained gastric mucosal biopsies contain abundant molecular information that could inform <em>Helicobacter pylori</em> (<em>H. pylori</em>) eradication therapy. However, the lack of point-of-care tools for processing solid tissue limits rapid, on-site genotyping, leading to empirical therapy and higher eradication failure rates. Here, we present the SlipChip-based On-site and User-friendly Testing (SCOUT) together with the restriction enzyme–assisted CRISPR/Cas12a (RCut) method for point-of-care genotyping from raw endoscopic gastric biopsies. This fully automated platform enables analysis of key virulence genes and resistance-associated single-nucleotide variants (SNVs) at mutant allele frequencies down to 0.1 %. Results were available within 1 h, enabling actionable molecular profiling during the same endoscopic encounter. In 159 clinical validations, SCOUT showed concordant virulence profiling with quantitative PCR (κ = 0.978) and accurately detected clarithromycin and levofloxacin resistance mutations with up to 100 % sensitivity and specificity. These results demonstrate that SCOUT bridges the gap between diagnostic sampling and informed eradication strategies, providing a scalable framework for point-of-care molecular genotyping.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"299 ","pages":"Article 118438"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146076544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imidazole functionalized nanozyme for deep detoxification and machine-learning-assisted intelligent sensing of profenofos","authors":"Xiaochen Liao,&nbsp;Bai Li,&nbsp;Xiao Wang,&nbsp;Li Wang,&nbsp;Yang Chen","doi":"10.1016/j.bios.2026.118440","DOIUrl":"10.1016/j.bios.2026.118440","url":null,"abstract":"<div><div>The excessive use of profenofos (PF) poses a significant threat to ecosystems and human health, making efficient degradation and detection technologies imperative. Despite the evident promise of nanozyme-based cascade catalytic systems, integrating such systems into a single nanozyme with high reactivity remains challenging. In this study, we have developed a luminescent nanozyme, Zn/MOF-808@Im/Cu-MOF, which possesses dual phosphatase and laccase activities for PF detoxification and intelligent sensing. Zn²⁺ doping enhances the Lewis acidity of MOF-808, promoting the hydrolysis of PF to 4-bromo-2-chlorophenol (BCP). Imidazole (Im) functionalization bridges MOFs and mimics the coordination environment of natural enzymes to accelerate proton and electron transfer, thereby boosting both hydrolytic and oxidative activities of the nanozyme and driving the oxidation of BCP to quinone intermediates. The quinone intermediates with 4-aminoantipyrine (4-AP) to generate a red product with a characteristic absorption peak and quench the fluorescence of Zn/MOF-808@Im/Cu-MOF, achieving colorimetric and fluorometric detection of PF. Utilizing the Residual Network 50 with Convolutional Block Attention Module (ResNet50-CBAM) neural network model, a machine learning-assisted intelligent sensing platform was further developed to enhance the practicability of PF detection. This study presents a novel approach for the detoxification and intelligent sensing of organophosphorus pesticides (OPs).</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"299 ","pages":"Article 118440"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146076602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}