Biosensors and Bioelectronics最新文献

{"title":"Synergistic catalysis of triethylamine by Pd nanocubes/Au nanoclusters heterostructure: An enhanced Au nanoclusters-triethylamine electrochemiluminescence system for As3+ aptasensing","authors":"Cheng Xu ,&nbsp;Jiali Huang ,&nbsp;Jiayi Liu ,&nbsp;Yinhui Yi ,&nbsp;Yao Zhu ,&nbsp;Deke Xing ,&nbsp;Libo Li ,&nbsp;Tianyan You","doi":"10.1016/j.bios.2026.118483","DOIUrl":"10.1016/j.bios.2026.118483","url":null,"abstract":"<div><div>Enhancing the electrochemiluminescence (ECL) of Au nanoclusters (AuNCs)-based system is a crucial approach for broadening their applications. Unlike traditional strategies that relied solely on coreaction accelerators to promote coreactant oxidation, this work innovatively adopted a “luminophore-coreaction accelerator” synergistic catalysis strategy to enhance the ECL of the AuNCs-Triethylamine (TEA) system. Specifically, Pd nanocubes (PdNCs), serving as a coreaction accelerator, formed a heterostructure with AuNCs on the working electrode surface. This structural design enhanced the ECL signal of the AuNCs-TEA system via two mechanisms. On one hand, PdNCs acted as efficient “electron traps” to capture electrons injected from TEA into the lowest unoccupied molecular orbital (LUMO) of AuNCs, thus significantly promoting the oxidation of TEA. On the other hand, the incorporation of PdNCs effectively improved the electrochemically active surface area of AuNCs, thus providing more active sites to catalyze the oxidation of TEA. Together, these two mechanisms accelerated the generation of TEA free radicals (TEA^•) and the subsequent excited state of AuNCs (AuNCs*), thereby enhancing the ECL of the system. As an application, the developed ECL aptasensor exhibited favorable analytical performance for As³⁺. This study has opened up a new way to improve the ECL properties of metal nanoclusters.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"300 ","pages":"Article 118483"},"PeriodicalIF":10.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146136978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogel molecularly imprinted polymer–based electrochemical sensor on gold-modified screen-printed carbon electrodes for stable and selective HbA1c detection","authors":"Launa Silky Karenindra Rokhmat ,&nbsp;Irkham ,&nbsp;Putra Rafli Ramdani ,&nbsp;Serly Zuliska ,&nbsp;Wulan Khaerani ,&nbsp;Dika Apriliana Wulandari ,&nbsp;Adisyahputra ,&nbsp;Santhy Wyantuti ,&nbsp;Iman Permana Maksum ,&nbsp;Yasuaki Einaga ,&nbsp;Genki Ogata ,&nbsp;Yeni Wahyuni Hartati","doi":"10.1016/j.bios.2026.118466","DOIUrl":"10.1016/j.bios.2026.118466","url":null,"abstract":"<div><div>Glycated hemoglobin (HbA1c) is a key biomarker for long-term glycemic control and diabetes diagnosis. Common methods such as chromatography and immunoassays are accurate but require costly instrumentation, limiting their use in point-of-care settings. Electrochemical sensors offer a portable and sensitive alternative. This study integrates computational modeling with hydrogel-based molecularly imprinted polymer (MIP) engineering to achieve selective HbA1c recognition. Molecular docking coupled with molecular dynamics simulations guided the rational design, identifying optimal binding sites and confirming the structural stability of the monomer–template complex. A hydrogel MIP sensor was fabricated on a gold-modified screen-printed carbon electrode using 2-acrylamido-2-methyl-1-propanesulfonic acid as the functional monomer and ethylene glycol dimethacrylate as the crosslinker. Successful polymer formation and template removal were confirmed by FTIR and XPS, while SEM verified uniform deposition of gold particles and MIP layers. Electrochemical characterization was performed using K₃[Fe(CN)₆]as the redox probe. The developed SPCE/Au/MIPs sensor exhibited a wide linear range 10–10⁵ ng/mL and LOD 1.13 ng/mL. A strong imprinting factor (IF 4.96) and high selectivity (98.7 %) confirmed effective molecular recognition, while long-term stability was demonstrated by stable performance over 120 days at room temperature with 94 % signal retention. Real sample measurements showed 95–112 % recovery compared to NGSP-standardized HPLC, with good reproducibility, validating its practical applicability. Overall, hydrogel MIP platform provides a robust, sensitive, and durable approach for HbA1c monitoring, supporting good health and well-being through accessible diabetes diagnostics.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"300 ","pages":"Article 118466"},"PeriodicalIF":10.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146098707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-mechanism-driven dual-mode array based on a single PFC-1/QD probe enables AI-assisted on-site identification of biogenic amines and real-time food freshness monitoring","authors":"Lanqing Yang ,&nbsp;Shilin Li ,&nbsp;Rong Guo ,&nbsp;Baoqing Bai ,&nbsp;Ying Zhang ,&nbsp;Wenyan Yan ,&nbsp;Shan Liu ,&nbsp;Shasha Zhao ,&nbsp;Wenbo Lu ,&nbsp;Yukun Yang","doi":"10.1016/j.bios.2026.118501","DOIUrl":"10.1016/j.bios.2026.118501","url":null,"abstract":"<div><div>Biogenic amines (BAs) serve as critical indicators of food spoilage, necessitating portable and real-time monitoring approaches to ensure food safety. To this end, we developed a P-QDot-based fluorescence/colorimetric (FL/CL) dual-mode sensor array integrated with deep learning and smartphone imaging for the classification and quantification of five BAs and the assessment of food freshness. The P-QDot probe was prepared by physically mixing the hydrogen-bonded organic framework PFC-1 with glutathione-capped quantum dots (QD) in aqueous solution. The fluorescence response was governed by a synergistic effect involving aggregation-induced emission (AIE), photoinduced electron transfer (PET), and the inner filter effect (IFE), which were activated by hydrogen bonding between the probe and BAs. Concurrently, a new UV absorption band was generated by P-QDot under the alkaline conditions induced by the BAs, leading to the colorimetric response. Leveraging this dual-mode response mechanism, the P-QDot-based FL/CL sensing array facilitated rapid (&lt;30 s), sensitive, and simultaneous qualitative discrimination and quantitative detection of BAs. Furthermore, a smartphone platform integrated with the YOLOv12 algorithm was established, enabling on-site BAs classification. The practical application of this system was demonstrated by real-time monitoring of shrimp spoilage during storage at 4 °C and 25 °C, where visually recognizable fluorescence color changes and colorimetric signals accurately reflected the degree of spoilage. Additionally, a logic gate device was engineered to directly translate the complex sensor signals into three intuitive freshness levels (fresh, acceptable freshness, and spoiled). This work presented a powerful strategy for food freshness assessment, successfully merging intricate material design with portable intelligence.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"300 ","pages":"Article 118501"},"PeriodicalIF":10.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146155590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating efficient signal amplification and magnetic separation into a homogeneous electrochemical strategy: Achieving ultra-low background simultaneous detection of aflatoxin B1 and ochratoxin A","authors":"Chunyuan Tang ,&nbsp;Bingzheng Yuan ,&nbsp;Libo Li ,&nbsp;Lijun Luo ,&nbsp;Tianyan You","doi":"10.1016/j.bios.2026.118465","DOIUrl":"10.1016/j.bios.2026.118465","url":null,"abstract":"<div><div>Coexisting aflatoxin B1 (AFB1) and ochratoxin A (OTA) can produce synergetic toxic effects, particularly carcinogenicity, highlighting the importance of their simultaneous detection. Although homogeneous strategies can avoid cross-interference on the electrode surface during multi-target detection, the complexity of the signal amplification process and background interference remains a challenge. This study integrates an efficient signal amplification strategy and magnetic separation technology into a homogeneous electrochemical strategy for the simultaneous detection of AFB1 and OTA. First, taking advantage of the adsorption of Zr-based metal-organic framework (UiO-66) for electroactive dyes, the aptamer was combined with it via the stirring method, followed by enrichment of neutral red (NR)/methylene blue (MB) to prepare a signal amplification probe. Then, the probe capture was achieved through double-strand hybridization by using complementary DNA (cDNA)-functionalized Fe₃O₄@PtNPs. Once the target was present, the high specificity of the aptamer forced the release of the signal probe. Meanwhile, the external magnet can rapidly remove the background-interfering species that are independent of the target. By ingeniously integrating the advantages of low background and an efficient signal amplification strategy, this method successfully achieved highly sensitive simultaneous detection of AFB1 and OTA. The detection ranges for AFB1 and OTA were 1 pg/mL to 100 ng/mL and 500 fg/mL to 100 ng/mL, with detection limits as low as 0.51 pg/mL and 0.42 pg/mL, respectively. Overall, this strategy represents a satisfactory exploration towards achieving efficient and sensitive strategies for detecting numerous mycotoxins.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"300 ","pages":"Article 118465"},"PeriodicalIF":10.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146123049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Active-site rich nanostructure with dual-enhanced electron transfer for ultra-sensitive electrochemiluminescence detection of DNA methylation in colon cancer","authors":"Xiao Tian ,&nbsp;Yuting Hou ,&nbsp;Liang Luan ,&nbsp;Lingzhi Ye ,&nbsp;Junjie Yuan ,&nbsp;Yating Zhang ,&nbsp;Mingliang Li ,&nbsp;Xiangmeng Qu ,&nbsp;Nan Wan","doi":"10.1016/j.bios.2026.118470","DOIUrl":"10.1016/j.bios.2026.118470","url":null,"abstract":"<div><div>Traditional DNA methylation assays for colon cancer still face challenges of insufficient sensitivity, limited specificity, and low cost-efficiency in clinical samples. Herein, we develop an electrochemiluminescence biosensor based on a dual-enhanced electron transfer mechanism using DNA tetrahedral nanostructure probes for the ultrasensitive detection of methylated DNA related to colon cancer. The DNA tetrahedral structure probe overcomes the shortcomings of poor adaptability caused by the low binding efficiency and high spatial steric hindrance in complex clinical samples. The electrochemiluminescence biosensor uses active-site-rich TiO₂-Ti₃C₂ MXenes@AgNPs hybrid nanocomposites as the electro-active substrate. These nanocomposites exhibited accelerated electron transfer and shortened the reaction paths due to the intrinsic photoelectric activity of TiO₂ and the surface plasmon resonance (SPR) effect between AgNPs and TiO₂. Furthermore, the three-dimensional (3D) self-assembled AuNPs-ABEI nanoluminous spheres substantially improved the luminescence intensity by concentrating more AuNPs to accelerate electron transfer while immobilizing additional ABEI molecules. The dual-enhanced electron transfer strategy significantly accelerated the kinetics of interfacial electron transfer. Metal nanoparticles (such as AgNP, AuNP, and TiO₂) generate SPR-induced local electric fields under electrochemical excitation. The electric fields enhance the electron transfer and amplify the electrochemiluminescence intensity, thereby achieving ultra-sensitive detection of the methylated Septin9 gene with a detection limit as low as 8.2 aM. Clinical sample validation using clinical serum samples from healthy controls (n = 100) and colon cancer patients (n = 100) has shown that the biosensor achieves comparable performance to the gold standard method in terms of both sensitivity and specificity, demonstrating equivalent detection efficacy to pyrosequencing.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"300 ","pages":"Article 118470"},"PeriodicalIF":10.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146122987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ion-induced plasmonic microneedle patches for ultrasensitive SERS detection Hemoporfin in interstitial fluids","authors":"Dan Li ,&nbsp;Shuai Zhao ,&nbsp;Weida Zhang ,&nbsp;Yongsheng Li ,&nbsp;Tanemura Masaki ,&nbsp;Gang Ma ,&nbsp;Yusi Peng ,&nbsp;Yong Yang","doi":"10.1016/j.bios.2026.118778","DOIUrl":"10.1016/j.bios.2026.118778","url":null,"abstract":"<div><div>Currently, the “one-size-fits-all” therapeutic window strategy for Hemoporfin-mediated photodynamic therapy of port-wine stains overlooks intralesional heterogeneity and interindividual pharmacokinetic differences. Simultaneously, this strategy lacks real-time feedback and personalized adjustment mechanisms. Therefore, the real-time monitoring of hemoporfin distribution in blood vessel and interstitial fluid is very important for finding the best laser illumination time window depended on personalized characterizes including the height, weight and so on. This study developed an ultra-sensitive sensor by integrating surface-enhanced Raman spectroscopy technology with microneedles (SERS-MNs) for monitoring the distribution dynamics of hemoporfin in blood and interstitial fluid within animal skin. Specifically, calcium ions were employed as an aggregating agent to induce the aggregation of gold/silver nanocage particles on the microneedle surface, thereby forming abundant SERS-enhanced “hot spots”. Using this sensor, characteristic Raman fingerprint signals of hemoporfin were successfully obtained for the first time, with a detection limit as low as 50 pg/mL. Furthermore, the SERS-MNs sensor not only quantitatively detected hemoporfin in pig skin but also successfully captured characteristic hemoporfin Raman signals in mouse interstitial fluid, blood, and treated patient blood. This revealed the spatiotemporal distribution mechanism of hemoporfin within the vascular system and interstitial fluid. This advancement will aid in determining the optimal therapeutic window for patients, thereby preventing both overtreatment and undertreatment, ultimately achieve precise therapy characterized by “individualization, visualization, and dynamization”.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"308 ","pages":"Article 118778"},"PeriodicalIF":10.5,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147855201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-atom co anchored UiO-66 to boost electrochemiluminescence of MoS2 for bioanalysis","authors":"Jinwen Zhao ,&nbsp;Guomin Yang ,&nbsp;Yifan Ruan ,&nbsp;Ruo Yuan ,&nbsp;Shihong Chen","doi":"10.1016/j.bios.2026.118432","DOIUrl":"10.1016/j.bios.2026.118432","url":null,"abstract":"<div><div>Single-atom catalysts (SACs) hold significant promise for improving the electrochemiluminescence (ECL) efficiency. However, the preparation of SACs reported in the ECL field usually relies on carbon-nitrogen-based materials as substrates and requires high-temperature pyrolysis (&gt;800 °C), suffering from the limitation in the anchoring materials and the deficiency of insufficient active site density caused by high-temperature calcination. This work developed zirconium-based metal-organic framework UiO-66 as the carrier to successively anchor the Co single-atom (Co_SA) and the ECL emitter MoS₂ under mild conditions (not exceeding 200 °C). Co_SA not only can optimize the electronic structure of MoS₂ to effectively promote the formation of Co(IV)=O, but also can act as the co-reactant accelerator to efficiently catalyze the reduction of coreactant S₂O₈²⁻. Meanwhile, the Mo-O-Zr bimetallic sites formed between UiO-66 and MoS₂ can enhance the charge carrier mobility within MoS₂. As a result, the ECL emission of MoS₂ at −1.6 V was remarkably boosted when using step pulse as the electrochemical method. The synthesized MoS₂@Co_SA/UiO-66 composite integrated an aptamer recognition-driven cascaded polymerase amplification to achieve ultrasensitive ECL detection of p-Tau as crucial biomarker for Alzheimer's disease (AD). Co_SA/UiO-66 exhibits broad application prospects in improving ECL efficiency. MoS₂@Co_SA/UiO-66 builds a highly sensitive ECL sensing platform for detecting the biomarkers of AD and other diseases.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"299 ","pages":"Article 118432"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146076542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Routine monitoring of microRNAs in salivary exosomes using a cellulose nanofiber sheet","authors":"Taiga Ajiri ,&nbsp;Min Zhang ,&nbsp;Naoya Mizukami ,&nbsp;Mikiko Iida ,&nbsp;Shota Kawaguchi ,&nbsp;Yurie Sekihara ,&nbsp;Kunanon Chattrairat ,&nbsp;Zetao Zhu ,&nbsp;Yoshinobu Baba ,&nbsp;Hirotaka Koga ,&nbsp;Takao Yasui","doi":"10.1016/j.bios.2026.118436","DOIUrl":"10.1016/j.bios.2026.118436","url":null,"abstract":"<div><div>Liquid biopsy is a promising approach for non-invasive disease monitoring, offering the potential for frequent sampling and early risk assessment. Among various biofluids, saliva is particularly well-suited for daily sampling due to its ease of collection and minimal invasiveness. Here, we present a cellulose nanofiber sheet as a novel platform for the rapid, low-volume recovery and stable storage of miRNAs associated with small extracellular vesicles in saliva. The sheet captures vesicles via its nanoscale porous networks and preserves their contents at room temperature, enabling efficient extraction of vesicle-associated miRNAs. Compared to ultracentrifugation, the method demonstrated superior miRNA recovery and required only 10 μL of saliva and less than 1 min of processing. Analysis of salivary miRNA profiles from healthy individuals and cancer patients revealed distinct expression patterns, enabling identification of candidate cancer-related miRNAs. Furthermore, daily saliva sampling during 20 days demonstrated the feasibility of longitudinal miRNA profiling and highlighted the effects of factors such as food intake. These findings underscore the potential of cellulose nanofiber sheets as a practical tool for salivary miRNA monitoring, with applications in personalized health management, early risk detection, and decentralized testing environments.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"299 ","pages":"Article 118436"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146049224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roll-to-roll printed in-pad biosensors for colorimetric detection of key biomarkers in artificial vaginal fluid","authors":"Ziheng Wang ,&nbsp;Semin Kwon ,&nbsp;Tianhao Yu ,&nbsp;Yumin Dai ,&nbsp;Young L. Kim ,&nbsp;Chi Hwan Lee","doi":"10.1016/j.bios.2026.118433","DOIUrl":"10.1016/j.bios.2026.118433","url":null,"abstract":"<div><div>Gynecological conditions often lack accessible, non-invasive diagnostic tools, leading to delayed detection and treatment, especially in resource-limited settings. Vaginal discharge and menstrual blood offer abundant but underutilized biomarkers for diagnosing gynecological disorders. Although sanitary pads represent an ideal platform for continuous fluid monitoring, direct high-throughput fabrication of sensors onto commercial pads remains challenging. Here, we introduce a roll-to-roll (R2R) wax-printing method optimized for large-scale production of fluidic colorimetric sensor patches designed for integration into sanitary pads. Utilizing specially formulated wax inks, this scalable approach enables consistent, cost-effective sensor fabrication. The sensor patches were functionalized with colorimetric reagents, incorporating enzymatic sensing elements for uric acid and glucose and physicochemical indicators for pH and moisture. This configuration enabled robust multi-analyte detection with clear and interpretable visual outputs, observable directly by the naked eye or via smartphone-based analysis. Validation in artificial vaginal fluid (AVF) confirmed reliable sensor performance, underscoring a practical and scalable platform with potential to advance proactive women's health monitoring in both home-based and point-of-care settings.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"299 ","pages":"Article 118433"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146076541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-strain engineering in Pd metallene for ultrasensitive tetracycline electrochemical biosensing","authors":"Yi Wen ,&nbsp;Yinan Dong ,&nbsp;Xuejie Shen ,&nbsp;Xingyu Luo ,&nbsp;Ziyi Wang ,&nbsp;Ruirui Guo ,&nbsp;Zhituo Geng ,&nbsp;Peng Li ,&nbsp;Yongping Huang ,&nbsp;Xinyu Zhu ,&nbsp;Jiatong Liu ,&nbsp;Wangyang Fu ,&nbsp;Zehui Li ,&nbsp;Xiaoyan Zhang","doi":"10.1016/j.bios.2026.118443","DOIUrl":"10.1016/j.bios.2026.118443","url":null,"abstract":"<div><div>Precise modulation of strain and electronic structure via atomic-level Mo and Cr co-doping in Pd metallene (MoCr-Pdene) significantly enhances electrocatalytic tetracycline (TC) sensing. Conventional Pd-based sensors suffer from weak TC affinity and sluggish kinetics due to surface inertness and biofouling. MoCr-Pdene overcomes these limitations through synergistic dual-strain effects, including compressive strain (from Cr) stabilizing the lattice and tensile strain (from Mo) redistributing surface charge. This strain-charge synergy shortens the Pd-O adsorption distance (1.82 Å), enhances adsorption energy (0.86 eV), and accelerates electron transfer, enabling an ultra-low detection limit of 27 nM, which represents ten-fold improvement over commercial Pd/C. The co-doped metallene exhibits exceptional stability (retaining 80 % of its signal after 120 days) and anti-biofouling capability in matrices. Importantly, MoCr-Pdene serves as a dual-functional platform for clinical diagnostics and environmental monitoring, quantitatively assessing TC biodegradation by black soldier fly larvae (BSFL) and gut microbiota (degradation rates: 97.96 % in frass, 90.67 % in BSFL). These results pioneer atomic-scale strain-electronic engineering for next-generation antibiotic sensing and sustainable bioremediation.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"299 ","pages":"Article 118443"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146076603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}