Biosensors and Bioelectronics最新文献_第6页

Highly sensitive and portable detection of PD-L1+ exosomes using a smartphone-assisted colorimetric sensor 使用智能手机辅助比色传感器进行PD-L1+外泌体的高灵敏度和便携式检测

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-05-21 DOI: 10.1016/j.bios.2025.117592

Gang Bai , Zhichao Fan , Xiaoxin Zhao , Yan Dong , Congcong Tan , Liyuan Bai , Haoran Jiang , Taorui Liu , Jianjun Li , Xiang Zhao , Hua Yu

{"title":"Highly sensitive and portable detection of PD-L1+ exosomes using a smartphone-assisted colorimetric sensor","authors":"Gang Bai , Zhichao Fan , Xiaoxin Zhao , Yan Dong , Congcong Tan , Liyuan Bai , Haoran Jiang , Taorui Liu , Jianjun Li , Xiang Zhao , Hua Yu","doi":"10.1016/j.bios.2025.117592","DOIUrl":"10.1016/j.bios.2025.117592","url":null,"abstract":"<div><div>PD-L1<sup>+</sup> exosomes act as a useful biomarker in early cancer diagnosis, therapeutic monitoring, prognostic assessment, and immunotherapy for non-invasive liquid biopsy. There exists urgent clinical need for developing rapid, portable, and cost-effective immediate-response assays for PD-L1<sup>+</sup> exosomes. Here, we proposed a smartphone-assisted colorimetric sensor using the triple-helix molecular switch (THMS) combined with Y-shaped catalytic hairpin assembly (Y-CHA) reaction. Namely, this strategy initiates the Y-CHA cycle and forms Y-DNA G-quadruplex/Hemin DNAzyme with K<sup>+</sup> and Hemin after specific recognition of PD-L1<sup>+</sup> exosomes by THMS. Then, PD-L1<sup>+</sup> exosomes could be quantified via the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB)-mediated color change. The method demonstrates excellent specificity and sensitivity, with a limit of detection (LOD) of 5.45 × 10<sup>3</sup> particles/mL and an LOD of 8.56 × 10<sup>3</sup> particles/mL using smartphone analysis. With this strategy, we found that PD-L1<sup>+</sup> exosome levels were significantly elevated in the peripheral blood of patients with colorectal cancer liver metastases (CRLM), indicating great potential for identifying CRLM patients. Additionally, this colorimetric sensor can be used to quantify PD-L1<sup>+</sup> exosomes across wide range of cancers, which possess a great prospect in the point-of-care testing filed for cancer companion diagnosis.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"286 ","pages":"Article 117592"},"PeriodicalIF":10.7,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stimuli-responsive ‘On–Off’ SERS–darkfield bimodal plasmonic nanoprobes for selective cancer cell illumination 刺激响应的“开-关”sers -暗场双峰等离子体纳米探针用于选择性癌细胞照明

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-05-21 DOI: 10.1016/j.bios.2025.117615

Swati Tanwar , Peng Zheng , Lintong Wu , Ishan Barman

{"title":"Stimuli-responsive ‘On–Off’ SERS–darkfield bimodal plasmonic nanoprobes for selective cancer cell illumination","authors":"Swati Tanwar , Peng Zheng , Lintong Wu , Ishan Barman","doi":"10.1016/j.bios.2025.117615","DOIUrl":"10.1016/j.bios.2025.117615","url":null,"abstract":"<div><div>Early detection is essential for improving cancer treatment outcomes. Surface-enhanced Raman scattering (SERS) offers high sensitivity and molecular specificity but suffers from slow imaging speed when used independently. Here, we introduce stimuli-responsive bimodal SERS–dark-field (DF) nanoprobes for rapid and selective imaging of metastatic prostate cancer cells. These nanoprobes utilize legumain enzyme–responsive peptide coatings on plasmonic gold nanocubes and a bio-orthogonal self-condensation mechanism to facilitate enzyme-triggered nanoprobe aggregation. Upon internalization, the nanoprobes selectively aggregate in DU145 cells that overexpress legumain, while remaining uniformly dispersed in LNCaP cells with minimal legumain activity. DF microscopy quickly identifies these aggregates, significantly expediting subsequent SERS imaging. The engineered design of these nanoprobes ensures distinct vibrational signatures specifically correlated to enzyme activity, establishing a direct link between enzymatic presence and SERS signal generation. By combining the rapid localization capabilities of DF imaging with the molecular precision of SERS, our bimodal imaging strategy significantly surpasses conventional single-modal SERS techniques, providing a versatile platform for fast and accurate detection of various diseases through their distinctive enzymatic profiles.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"286 ","pages":"Article 117615"},"PeriodicalIF":10.7,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144178611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coreactant-free and single-potential-channel electrochemiluminescence from dual-thiols-stabilizer capped gold nanoclusters and its NIR immunoassay application 双硫醇-稳定剂覆盖金纳米团簇的无共物和单电位通道电化学发光及其近红外免疫分析应用

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-05-21 DOI: 10.1016/j.bios.2025.117617

Mengwei Li, Xuwen Gao, Xiaoxuan Ren, Luyao Ma, Yujiao Wang, Guizheng Zou

{"title":"Coreactant-free and single-potential-channel electrochemiluminescence from dual-thiols-stabilizer capped gold nanoclusters and its NIR immunoassay application","authors":"Mengwei Li, Xuwen Gao, Xiaoxuan Ren, Luyao Ma, Yujiao Wang, Guizheng Zou","doi":"10.1016/j.bios.2025.117617","DOIUrl":"10.1016/j.bios.2025.117617","url":null,"abstract":"<div><div>Ru(bpy)<sub>3</sub><sup>2+</sup>/tripropylamine (TPrA) is the sole electrochemiluminescence (ECL) system, which is being extensively employed in commercialized In Vitro Diagnosis. The coreactant ECL of Ru(bpy)<sub>3</sub><sup>2+</sup>/TPrA is of a multiple-channel emission and wide electrochemical potential window nature. Herein, a coreactant-free and single-potential-channel ECL strategy is proposed with a biocompatible nano-electrochemiluminophore, i.e., dihydrolipoic acid-capped Au nanoclusters (DHLA-AuNCs) as model, in which the metal-sulfur bonds between two sulfhydryls of DHLA and the Au element enable AuNCs with a S vacancies involved electron-rich and n-type nature for DHLA-AuNCs. The DHLA-AuNCs can be electrochemically oxidized, the annihilation reaction between exogenous holes and endogenous free-electrons can emit a kind of coreactant-free single-potential-channel ECL between +0.70 and + 1.20 V (<em>vs</em> Ag/AgCl) with the maximum emission potential of +0.91 V (<em>vs</em> Ag/AgCl) and the maximum emission wavelength of 778 nm. The ECL can be conveniently achieved, and selectively determine human carcinoembryonic antigen with the linear response range from 20 to 5000 pg/mL and the limit of detection of 5 pg/mL. The coreactant-free and single-potential-channel ECL of DHLA-AuNCs provides an alternative to the coreactant ECL of Ru(bpy)<sub>3</sub><sup>2+</sup>/TPrA for less electrochemical-interference and signal-crosstalk.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"286 ","pages":"Article 117617"},"PeriodicalIF":10.7,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144147133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integration of polyvalent aptamers and multifunctional gold-palladium nanoparticle driven rapid and ultra-sensitive triple-mode lateral flow assay detection for tetrodotoxin 多价适体与多功能金钯纳米粒子集成驱动河豚毒素快速超灵敏三模横向流动检测

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-05-21 DOI: 10.1016/j.bios.2025.117619

Yu Li , Shuo Qi , Peifang Chen , Chifang Peng , Zhouping Wang

{"title":"Integration of polyvalent aptamers and multifunctional gold-palladium nanoparticle driven rapid and ultra-sensitive triple-mode lateral flow assay detection for tetrodotoxin","authors":"Yu Li , Shuo Qi , Peifang Chen , Chifang Peng , Zhouping Wang","doi":"10.1016/j.bios.2025.117619","DOIUrl":"10.1016/j.bios.2025.117619","url":null,"abstract":"<div><div>The development of rapid and ultra-sensitive aptamer-based lateral flow assay (Apt-LFA) for the detection of small molecules remains a significant challenge. In this study, we developed a colorimetric-photothermal “turn on” mode LFA for detecting tetrodotoxin (TTX) by integrating magnetic polyvalent aptamers (MB@poly-Apts) and onion flower-like gold-palladium nanoparticles (MOGP). Guided by structural information of the aptamer, we employed a truncation optimization strategy to rationally develop the TTX-specific aptamer A36. The capture probe, MB@poly-Apts, was synthesized by modifying magnetic beads with a rigid DNA linker, the truncated aptamer A36, and a complementary linker. This poly-Apts exhibited an enhanced binding affinity (9.3 nM) by 4-fold and binding efficiency by 2-fold for TTX attributed to its unique design featuring polyvalent binding sites and gaps in the complementary structure. The signaling probe, MOGP, possessed a broad absorption spectrum, a multibranched structure, and bimetallic/polydopamine compositions, providing excellent colorimetric properties, catalytic activity, and photothermal performance. The developed MB@poly-Apts-assisted MOGP-based LFA achieved colorimetric, catalytically enhanced colorimetric, and photothermal modes of TTX detection with visual detection limits as low as 1, 0.01, and 0.025 ng/mL, respectively. This performance significantly outperforming traditional gold nanoparticle-based LFA. The Apt-LFA demonstrated ultra-sensitivity with good flexibility, positioning it as a promising platform for on-site testing for diverse applications.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"286 ","pages":"Article 117619"},"PeriodicalIF":10.7,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144147134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A battery-free wearable sweat lactate sensing patch for assessing muscle fatigue and recovery 一种无电池可穿戴的汗液乳酸传感贴片，用于评估肌肉疲劳和恢复

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-05-21 DOI: 10.1016/j.bios.2025.117616

Jiachen Zhu , Tao Wang , Hai Li , Lu Yin , Guorui Li , Gang Chen , Xingchi Liu , ShuTing Liu , XianYi Zhu , Hui Liu , Shangbin Xi , Wei Cai , Liang Peng , Shaopeng Ma , Luwen Wang

{"title":"A battery-free wearable sweat lactate sensing patch for assessing muscle fatigue and recovery","authors":"Jiachen Zhu , Tao Wang , Hai Li , Lu Yin , Guorui Li , Gang Chen , Xingchi Liu , ShuTing Liu , XianYi Zhu , Hui Liu , Shangbin Xi , Wei Cai , Liang Peng , Shaopeng Ma , Luwen Wang","doi":"10.1016/j.bios.2025.117616","DOIUrl":"10.1016/j.bios.2025.117616","url":null,"abstract":"<div><div>Long-term sports and rehabilitation training require effective monitoring of muscle fatigue and recovery. Blood lactate fails to accurately reflect muscle fatigue level over extended period. Sweat lactate is a promising alternative, but its potential for assessing multi-day muscle fatigue remains unexplored. Here, we present a method for assessing multi-day muscle fatigue and recovery utilizing a high-performance, battery-free, and practical sweat lactate sensing patch. The wearable patch integrates replaceable lactate sensors and electronics that can be powered and transmits data wirelessly via near-field communication. A high-performance lactate sensor based on a novel three-dimensional microstructured electrode with a Pt/CNT-Pt composite was investigated. The synergistic effect between the electrode material and structure imparts an ultra-high sensitivity of 9.76 μA mM<sup>−1</sup> cm<sup>−2</sup> within a sufficient linear range of 0.1–30 mM. The electrode features a through-hole array to ensure sufficient oxygen supply for enzyme catalysis during skin attachment, ensuring reliable signal output. The sweat lactate profiles under different conditions were studied. Multi-day exercise experiments demonstrate a trend in initial sweat lactate levels, which is absent in initial blood lactate levels. Monitoring sweat lactate during rehabilitation training can also guide training programs. The feasibility of assessing muscle fatigue during multi-day exercise through sweat lactate levels was demonstrated. This work presented a practical method to determine lactate response and muscle fatigue level during exercise towards generating personalized training plans.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"286 ","pages":"Article 117616"},"PeriodicalIF":10.7,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144116488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ZnIn2S4/TiO2 NRs heterojunction-multifunctional hydrogel hybrid for ultrasensitive photoelectrochemical immunoassay ZnIn2S4/TiO2 NRs异质结-多功能水凝胶杂化物用于超灵敏光电化学免疫分析

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-05-21 DOI: 10.1016/j.bios.2025.117618

Meng-Jiao Lu , Shan-Qi Zhang , Sheng Wang , Kun-Hong Zhao , Wishwajith Kandegama , Mo-Xian Chen , Nan Jiang , Xiang-Yang Li

{"title":"ZnIn2S4/TiO2 NRs heterojunction-multifunctional hydrogel hybrid for ultrasensitive photoelectrochemical immunoassay","authors":"Meng-Jiao Lu , Shan-Qi Zhang , Sheng Wang , Kun-Hong Zhao , Wishwajith Kandegama , Mo-Xian Chen , Nan Jiang , Xiang-Yang Li","doi":"10.1016/j.bios.2025.117618","DOIUrl":"10.1016/j.bios.2025.117618","url":null,"abstract":"<div><div>The prevalence of tobacco virus diseases has caused serious losses to the tobacco industry, making the development of efficient, sensitive, and convenient virus detection technologies increasingly important. Photoelectrochemical biosensors have been widely used in various fields due to their advantages, including low background signal, convenient operation, low cost, and fast response. However, few studies have explored the use of photoelectric sensing analysis technology for the detection of plant viruses. In this study, we propose a novel biosensor based on semiconductor heterojunction nanomaterials for the detection of plant virus tobacco mosaic virus (TMV). The sensor utilizes a ZnIn<sub>2</sub>S<sub>4</sub>/Titanium dioxide nanorod arrays (TiO<sub>2</sub> NRs) heterojunction nanocomposite, modified with fluorine-doped tin oxide (FTO), as a photoelectrode. This photoelectrode triggers a gelation reaction of the hydrogel by coupling the Ca<sup>2+</sup> generated in the 96-well plate with TMV immune recognition events, thereby altering the interfacial mass transfer on the photoelectrode surface. This gelation significantly hinders the light absorption of the ZnIn<sub>2</sub>S<sub>4</sub>/TiO<sub>2</sub> NRs/FTO photoelectrodes, ultimately resulting in a corresponding change in the output current signal. The biosensor demonstrates excellent analytical performance, with a detection limit of 0.01 pg mL<sup>−1</sup> for TMV. This work provides a novel approach for the analysis and detection of plant viruses, holds significant potential for further development. It is expected to provide a general platform for detection and analysis of plant viruses, agricultural pollutants, food residues and other fields in the future.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"286 ","pages":"Article 117618"},"PeriodicalIF":10.7,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trace protein detection by graphene-integrated terahertz meta-sensor and enhanced sensitivity using gold nanoparticles-assisted 石墨烯集成太赫兹元传感器检测痕量蛋白质及金纳米粒子辅助增强灵敏度

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-05-20 DOI: 10.1016/j.bios.2025.117612

Tongling Wang , Ziqi Li , Mingyao Wang , Changshun Wu , Maosheng Yang , Xiuwei Yang , Li Wang , Kuilong Li , Zhou Yang , Wenjing Zheng , Lanju Liang , Haiyun Yao , Xin Yan , Jianquan Yao

{"title":"Trace protein detection by graphene-integrated terahertz meta-sensor and enhanced sensitivity using gold nanoparticles-assisted","authors":"Tongling Wang , Ziqi Li , Mingyao Wang , Changshun Wu , Maosheng Yang , Xiuwei Yang , Li Wang , Kuilong Li , Zhou Yang , Wenjing Zheng , Lanju Liang , Haiyun Yao , Xin Yan , Jianquan Yao","doi":"10.1016/j.bios.2025.117612","DOIUrl":"10.1016/j.bios.2025.117612","url":null,"abstract":"<div><div>With the rapid development of terahertz (THz) biosensing technology, the research on trace protein detection via THz metasurfaces has attracted significant attention. Here, we propose a graphene-integrated toroidal split-ring resonator (TSRR) THz meta-sensor for detecting protein at trace levels based on the Dirac property of chemical vapor deposition (CVD) graphene. The experimental results show that the designed meta-sensor can realize the detection of midkine with a 125 pg/mL limit of detection (LOD). Furthermore, benefiting from the charge accumulation generated by the local surface plasmon resonance of gold nanoparticles (AuNPs) to induce the enhancement of the local electric field of graphene, trace carcinoembryonic antigen (CEA) detection with an LOD of 10 pg/mL is achieved assisted by CEA antibody-AuNPs. The proposed meta-sensor realizes picogram-level protein detection, providing new ideas for applying metasurface-based THz sensors in biosensing.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"286 ","pages":"Article 117612"},"PeriodicalIF":10.7,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144131032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A CRISPR/Cas12a biosensor for portable and accessible detection of Salmonella typhimurium via multi-indicator pH millidisc colorimetry and smartphone imaging platform 基于多指示剂pH比色法和智能手机成像平台的便携式鼠伤寒沙门菌检测CRISPR/Cas12a生物传感器

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-05-20 DOI: 10.1016/j.bios.2025.117611

Miaolin Duan , Guoqiang Li , Jingqi Shen , Ruitong Dai , Xingmin Li , Zunying Liu , Fei Jia

{"title":"A CRISPR/Cas12a biosensor for portable and accessible detection of Salmonella typhimurium via multi-indicator pH millidisc colorimetry and smartphone imaging platform","authors":"Miaolin Duan , Guoqiang Li , Jingqi Shen , Ruitong Dai , Xingmin Li , Zunying Liu , Fei Jia","doi":"10.1016/j.bios.2025.117611","DOIUrl":"10.1016/j.bios.2025.117611","url":null,"abstract":"<div><div>Conventional colorimetric CRISPR/Cas methods rely on a single chromogenic substrate and bulky and specialized signal detection instrument, which hinder their practical application. Herein, a portable and accessible CRISPR/Cas12a biosensor was for the first time reported to sensitively quantify <em>Salmonella enterica</em> serovar <em>typhimurium</em> (<em>S. typhimurium</em>), utilizing a multi-indicator pH millimeter disc (millidisc) for signal visualization, combined with a smartphone-based imaging platform for signal readout. The pH millidisc, composed of multiple indicators, possessed sensitive pH responsiveness and exhibited diverse color changes. The self-developed RGB mini-program, named DeepFood, was designed for portable smartphone use, featuring user-friendly operation and trend visualization for preliminary result analysis. Based on the RGB signal variation pattern with <em>S. typhimurium</em>, a distinct <em>S</em><sub>enh</sub> signal type was designed, enhancing the signal-to-noise ratio from 3.38 to 7.11. Compared to the R signal type, the <em>S</em><sub>enh</sub> signal type improved detection sensitivity by 36.23-fold (7.26 CFU/mL) in 0.01 M PBS buffer and 15.53-fold (1.41 × 10<sup>2</sup> CFU/mL) in chicken. The proposed biosensor offers significant improvements in detection sensitivity and practical applicability, with potential in food safety and environmental protection.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"286 ","pages":"Article 117611"},"PeriodicalIF":10.7,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Graphene-mediated surface enhanced Raman spectroscopy for DNA Detection&hybridization: Breakthroughs and challenges 石墨烯介导的表面增强拉曼光谱用于DNA检测和杂交：突破和挑战

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-05-20 DOI: 10.1016/j.bios.2025.117610

Vlad Cucuiet , Dana Maniu , Simion Astilean , Marc Lamy de la Chapelle , Monica Focsan

{"title":"Graphene-mediated surface enhanced Raman spectroscopy for DNA Detection&hybridization: Breakthroughs and challenges","authors":"Vlad Cucuiet , Dana Maniu , Simion Astilean , Marc Lamy de la Chapelle , Monica Focsan","doi":"10.1016/j.bios.2025.117610","DOIUrl":"10.1016/j.bios.2025.117610","url":null,"abstract":"<div><div>In response to the growing demand for advanced DNA research, the need for refined scientific methodologies has become increasingly evident. This review provides a comprehensive synthesis of recent advancements in the application of graphene-mediated Surface Enhanced Raman Spectroscopy (G-SERS) for DNA detection and hybridization, highlighting its significant potential in biomedical fields particularly gene detection and emphasizing its crucial role in advancing genetic research. More importantly, it emphasizes the pivotal role of Au and Ag nanoparticles, with varying shapes and sizes, in enhancing signal intensity and improving analytical performance, highlighting the significant contributions of G-SERS in enhancing both the sensitivity and selectivity of DNA analysis. Furthermore, our review explores innovative hybrid approaches that integrate SERS with Quartz Crystal Microbalance (QCM) and Atomic Force Microscopy (AFM), marking a transformative shift in analytical capabilities. Overall, this review offers valuable insights into the evolving landscape of DNA analysis technologies, paving the way for future advancements in genetic research.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"286 ","pages":"Article 117610"},"PeriodicalIF":10.7,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas12a-triggered electrochemiluminescence biosensor to ultrasensitive detect herpes simplex virus via self-enhanced near-infrared selenium-based polymer dots 基于自增强近红外硒基聚合物点的CRISPR/ cas12a触发电化学发光生物传感器超灵敏检测单纯疱疹病毒

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-05-20 DOI: 10.1016/j.bios.2025.117597

Yaoyao Xu , Rongxiu Deng , Xue Liu, Yuyang Zhou

{"title":"CRISPR/Cas12a-triggered electrochemiluminescence biosensor to ultrasensitive detect herpes simplex virus via self-enhanced near-infrared selenium-based polymer dots","authors":"Yaoyao Xu , Rongxiu Deng , Xue Liu, Yuyang Zhou","doi":"10.1016/j.bios.2025.117597","DOIUrl":"10.1016/j.bios.2025.117597","url":null,"abstract":"<div><div>Herpes simplex virus (HSV) as one of the most popular herpesviruses exhibits nearly identical clinical symptoms among the family of herpesvirus species and it is urgent to develop high specific biosensors to realize accurate detection of HSV. CRISPR/Cas12a systems with programmability and high specificity could serve an essential role in accurate diagnosis of HSV in complex samples. Herein, a novel near-infrared selenium-based polymer dots with self-enhanced effect were unprecedentedly designed and successfully synthesized in this work, and an ultrasensitive biosensor has been constructed together with the CRISPR/Cas12a system for the specific detection of HSV. Notably, the as-prepared selenium-based polymer dots exhibited near-infrared emission with a peak at 760 nm, which could significantly minimize background noise and achieve high sensitivity. The proposed biosensor demonstrated a wide linear range from 1 fM to 1 nM and the limit of detection as low as 0.1 fM (S/N = 3). Undoubtedly, the CRISPR/Cas12a-triggered ECL biosensor proposed in this work could provide a simple, sensitive, and environmentally friendly approach to detect HSV in clinical applications.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"286 ","pages":"Article 117597"},"PeriodicalIF":10.7,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0