Biosensors and Bioelectronics最新文献

{"title":"Inner filter effect regulated upconversion sensing platform for non-invasive detection of two gastric cancer relevant substances","authors":"Guirong Cheng ,&nbsp;Xinyu Shao ,&nbsp;Peiyuan Lin,&nbsp;Jingwen Zhang,&nbsp;Lixia Lu","doi":"10.1016/j.bios.2025.118029","DOIUrl":"10.1016/j.bios.2025.118029","url":null,"abstract":"<div><div>Gastric cancer is the fifth most common cancer in the world. The concentrations of D-Pro and D-Ala in the saliva of patients with early gastric cancer were significantly higher than those of healthy people. Urease has also been found to be closely associated with gastric cancer. Therefore, monitoring the concentration of D-amino acids and urease is of great significance for the diagnosis and treatment of gastric cancer. In this study, multi-peak emission UCNPs (ES-UCNPs) was synthesized based on emulsion self-assembly strategy. Polyethylenimine (PEI) modified copper-based Prussian blue analogues (PEI-CuFc) exhibit enhanced the stability and peroxidase-like catalytic activity. And we developed a colorimetric-luminescent dual-mode sensing platform for two gastric cancer relevant substances by utilizing PEI-CuFc nanozymes to regulate the luminescence spectrum through IFE mechanism. Notably, besides serving as the foundation for emulsion self-assembly, sodium dodecyl sulfate (SDS) effectively improved the catalytic activity of PEI-CuFc, significantly amplifying the detectable signal and thereby enhancing assay sensitivity. To our knowledge, this study presents the first report of simultaneous detection of these two gastric cancer relevant substances in a single assay. The detection limits fall within the clinically relevant concentration range of early gastric cancer biomarkers, offering a promising approach for non-invasive early diagnosis of gastric cancer.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118029"},"PeriodicalIF":10.5,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Donor-acceptor photoelectrochemical strategy for efficient detection and degradation chloramphenicol based on dual-porphyrin Z-scheme heterostructure","authors":"Xiaoping Bai ,&nbsp;Wei Zhang ,&nbsp;Cong Tang ,&nbsp;Yaya Wang ,&nbsp;Qing Shen ,&nbsp;Shijun Shao ,&nbsp;Guangxiu Liu ,&nbsp;Shuqing Dong","doi":"10.1016/j.bios.2025.118026","DOIUrl":"10.1016/j.bios.2025.118026","url":null,"abstract":"<div><div>Nowadays, developing a new strategy to construct highly efficient photoelectric active materials is imperative but challenging for photoelectrochemical (PEC) technology applications in the fields of pollutant analysis and degradation. Inspired by natural photosynthesis, a novel Z-scheme heterojunctions-based cathodic “signal-on” PEC aptasensor was constructed based on the electron donor-acceptor (D-A) strategy. The Z-scheme heterojunctions were fabricated by self-assembly of porphyrin and copper-porphyrin metal-organic framework (Cu-PorMOF), which possessed excellent electron-donor properties. The D-A strategy is based on the interaction between the photocathode and electron acceptor analyte, which has the advantages of simple construction of the sensing interface and strong anti-interference capability, highlighting the broad applicability of PEC sensing technology in the analysis of complex samples. Chloramphenicol (CAP) was selected as a representative of electron-acceptor analyte for the validation of the PEC sensing ability. The experimental results showed that the constructed PEC sensor exhibited a high sensitivity with a wide linear range from 2 to 200 nM and a low detection limit of 0.5 nM (3 S/N). Furthermore, the heterojunctions could efficiently degrade CAP, and the degradation processes were in situ online monitored using scanning photoelectrochemical microscopy technology. Overall, this work provides an integrated strategy to detect and degrade CAP by engineering the interfacial structure of heterojunctions.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118026"},"PeriodicalIF":10.5,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"‘Inner membrane - outer gel’ PEDOT:PSS/MXene composite material enhances the extracellular electron transfer process by promoting biofilm growth","authors":"Bingyin Zhu,&nbsp;Xu Pan,&nbsp;Hongxiang Guo,&nbsp;Ye Chen,&nbsp;Qing Wen","doi":"10.1016/j.bios.2025.118021","DOIUrl":"10.1016/j.bios.2025.118021","url":null,"abstract":"<div><div>Against the backdrop of surging global energy demand, microbial fuel cells (MFCs) have garnered significant attention for their potential in green energy conversion. To solve the problem of low efficiency of long-distance electron transfer by microorganisms at the anode interface of MFCs, we propose a novel ‘inner membrane - outer gel’ PEDOT:PSS/MXene dual-phase hydrogel electrode. The electrode is fabricated via electrochemical polymerization, which further enables it to use a flexible heterogeneous interface to promote three-dimensional (3D) biofilm generation. Experimental results indicate that the composite anode exhibits a charge transfer resistance (R_ct) as low as 4.71 Ω, with a maximum power density of 4.55 ± 0.17 W m^-2, which is 1.8 times the maximum power density of a pure hydrogel (only PEDOT:PSS hydrogel). Furthermore, 16S rRNA sequencing revealed that the relative abundance of Geobacter increased to 62.22 %, indicating a significant enrichment of electrogenic microorganisms. Molecular docking simulations further elucidated the electrostatic complementarity and hydrogen bonding interactions between PEDOT and the OmcZ protein, providing theoretical support for efficient electron transfer between conductive nanowires and electrodes within biofilms. Overall, this study provides both experimental and theoretical evidence for the feasibility of the ‘inner membrane - outer gel’ electrode in enhancing MFC performance, offering new insights into the design of high-performance conductive bioelectrodes in microbial energy conversion devices.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118021"},"PeriodicalIF":10.5,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A smartphone platform developed with AI assistance for ultrasensitive dual-mode detection of tetracycline using Au@ZnO/Pt nanozymes","authors":"Yanan Wang ,&nbsp;Yi Huang ,&nbsp;Chang Li ,&nbsp;Yatie Xiao ,&nbsp;Yanfang Wu ,&nbsp;Mingcong Rong","doi":"10.1016/j.bios.2025.118024","DOIUrl":"10.1016/j.bios.2025.118024","url":null,"abstract":"<div><div>Tetracycline (TC), a broad-spectrum antibiotic widely used in both human and veterinary medicine, has raised serious environmental and public health concerns due to its widespread misuse. Consequently, there is an urgent need to develop rapid and user-friendly methods for TC detection. In this study, we developed a dual-mode sensing platform based on Au@ZnO/Pt nanoparticles with oxidase-like activity. These nanoparticles catalyzed the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to a blue-colored product, which was visibly inhibited in the presence of TC, enabling sensitive colorimetric detection of TC with a limit of detection (LOD) as low as 0.34 nM. Simultaneously, the Zn²⁺ ions in the nanoparticles formed stable chelates with TC, resulting in a significant turn-on green fluorescence response, achieving a fluorescent detection method with an LOD of 0.48 nM. By integrating these two mechanisms, we established a dual-mode sensing strategy combining colorimetric and fluorescence outputs for the rapid (≤5 min), sensitive, and reliable detection of TC in real water samples. Furthermore, with the assistance of AI, we developed a smartphone application and integrated it with test strips embedded with Au@ZnO/Pt nanoparticles, providing a portable and practical solution for on-site TC monitoring.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118024"},"PeriodicalIF":10.5,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"pH sensing and imaging in living cells based on fluorescence lifetime of carbon dot nanosensors","authors":"Sergii Kalytchuk ,&nbsp;Tomaš Malina ,&nbsp;Filip Mravec ,&nbsp;Kateřina Poláková ,&nbsp;Lukáš Zdražil ,&nbsp;Štěpan Kment ,&nbsp;Andrey L. Rogach ,&nbsp;Michal Otyepka ,&nbsp;Radek Zbořil","doi":"10.1016/j.bios.2025.118022","DOIUrl":"10.1016/j.bios.2025.118022","url":null,"abstract":"<div><div>The pH value is one of the most frequently measured chemical parameters, yet developing nanometric sensors capable of accurately mapping pH distribution and dynamics with high spatial and temporal resolution remains a significant challenge. Such sensors are vital for advancing our understanding of numerous physiological and pathological processes. Nanoparticle-based sensors, commonly referred to as nanosensors, represent a promising class of optical sensors, with fluorescence lifetime-based probes offering superior sensitivity and quantitative reliability. However, existing pH nanosensors relying on fluorescence lifetime are challenging to synthesize and often suffer from poor biocompatibility, narrow pH response ranges, low stability, and calibration-dependent performance. Here, we overcome these limitations by introducing a water-dispersible pH nanosensor based on fluorescence lifetime of colloidal carbon dots (CDs) derived <em>via</em> a one-step reaction from a single precursor Rhodamine B. These CDs are biocompatible, non-toxic, and stable in highly acidic/basic conditions, which makes them well-suited for intracellular applications. The intrinsic fluorescence lifetime of these CDs exhibits a pseudo-linear, self-referencing response across exceptionally broad pH range (1–11), driven by pH-induced transformations of their electronic structure occurring during protonation and deprotonation of CD surface. By applying micrometer-resolution, quantitative pH imaging <em>via</em> fluorescence lifetime imaging microscopy, we demonstrate how CDs are preferentially sequestered in lysosomes of human skin fibroblasts, enabling precise quantification of inhibitor-induced pH changes within these organelles. Our findings highlight a significant potential of the CD nanosensors for precise monitoring of lysosomal pH in living cells, offering broad utility in biomedical research and potential studies of pH-associated cellular dysfunction.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118022"},"PeriodicalIF":10.5,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145190553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultra-efficient label-free cell death detection based on multifunctional colorimetric fluorescent in-situ silver nanocluster/graphene nanohybridzyme and its application in environmental biological samples and cellular imaging","authors":"Fatemeh Molaabasi ,&nbsp;Nasim Mohseni ,&nbsp;Behnam Hajipour-Verdom ,&nbsp;Arash Agheli ,&nbsp;Ali Akbar Moosavi-Movahedi ,&nbsp;Reza Mahdavian ,&nbsp;Afsaneh Roshanfekr ,&nbsp;Mojtaba Shamsipur ,&nbsp;Hossein Naderi-Manesh ,&nbsp;Reza Gholami ,&nbsp;Vahid Yousefi ,&nbsp;Amirhosein Kefayat ,&nbsp;Ramin Sarrami‐Forooshani","doi":"10.1016/j.bios.2025.118020","DOIUrl":"10.1016/j.bios.2025.118020","url":null,"abstract":"<div><div>In this study an efficient label-free multifunctional probe based on <em>in situ</em> hemoglobin-stabilized silver nanocluster/graphene nanohybridzymes (Hb-AgNCs/graphene NHZs) was designed and employed for dual-modal naked-eye colorimetric and fluorometric detection of cell death biomarkers involved in ferroptosis, cuproptosis, and apoptosis. The interesting feature of developed NHZs is the colorimetric speciation of “turn-on” Fe²⁺, Cu²⁺, and Cyt <em>c</em> and “turn-off” cysteine (Cys) and glutathione (GSH), and its speciation ability of Fe²⁺ from Fe³⁺as well. The presence of analytes causes a colorimetric limit of detection of 10.8–191 nM and fluorometric detection limit from 0.25 to 121 nM in the appropriate linear ranges, with fast times ranging 5–20 min. The designed NHZs were successfully employed for fluorescence imaging of HT29 human colon cancer and also quantitatively validated in just 2 μL of cell lysates, environmental, and biological samples. Compared to the semi-quantitative conventional Western blot and other cell death assays, the novelty of presented probe is low cost, simple preparation, high sensitivity, multifunctionallity, and dual-modality with the speciation and quantitative ability without using any targeting agent, which makes it appropriate for dual-modal tracking cell death processes and drug evaluation in a very short time.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118020"},"PeriodicalIF":10.5,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfluidic paper-based fluorescent sensing device using citric acid derived carbon dots-tungsten sulfide spheres for on-site detection of secnidazole","authors":"Shan Arif ,&nbsp;Mahjabeen Hassan ,&nbsp;Sufian Rasheed ,&nbsp;Naseer Ahmad ,&nbsp;Batool Fatima ,&nbsp;Syed Ghulam Musharraf ,&nbsp;Dilshad Hussain","doi":"10.1016/j.bios.2025.118025","DOIUrl":"10.1016/j.bios.2025.118025","url":null,"abstract":"<div><div>Secnidazole, a second-generation nitroimidazole, is widely used to treat various infections, including bacterial vaginosis, amoebiasis, and giardiasis, particularly in the treatment of female genital infections. Herein, a smartphone-based sensing platform has been developed in conjunction with a microfluidic-based paper device, enabling the visual, quick, sensitive, and quantitative analysis of secnidazole in real-time through fluorescence \"Turn off\". In addition to its high sensitivity for label-free and on-site detection of secnidazole, the proposed sensing device demonstrated a prominent anti-interference capability, ensuring accurate on-site identification. The N-CDs@WS₂-based fluorescent sensor exhibited a limit of detection (LOD) of 0.117 μM (117 nM) and a limit of quantification (LOQ) of 0.3573 μM (357 nM) for secnidazole. The portable sensor effectively enabled real-time monitoring of secnidazole in serum samples, yielding encouraging outcomes and paving the way for its accurate on-site detection. The detection efficiency of the sensor is validated by the World Health Organization (WHO) recommended LC-MS and UPLC methods. As indicated by the recoveries from UPLC, LC-MS, and N-CDs@WS₂-based sensing device, the proposed sensor can quantitaively analyze secnidazole in real samples.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118025"},"PeriodicalIF":10.5,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shattering kinetic constraints: hierarchically activatable DNAzyme nanoantennas for in situ mRNA imaging and precise cancer theranostics","authors":"Qian Han,&nbsp;Shi Wang,&nbsp;Li-juan Wang,&nbsp;Chun-yang Zhang","doi":"10.1016/j.bios.2025.118016","DOIUrl":"10.1016/j.bios.2025.118016","url":null,"abstract":"<div><div>Cancer progressions are complicated events with involvement of abnormal expression of different RNAs. DNAzyme-based imaging-guided gene therapy offers promising prospects for effectively treating serious diseases, but it is constrained by unconditional activation, inefficient delivery, and limited amplification capacity. Herein, we construct shattering kinetic constraints: hierarchically activatable DNAzyme nanoantennas for in situ mRNA imaging and precise cancer theranostics. This nanosystem incorporates multifunctional modules including an aptamer-mediated tumor-target module, an i-motif-gated recognition module, a metal ion-activated amplification module, and an antisense oligonucleotide (ASO)-induced therapy module. Once encountering cancer cells, hairpin 1 (H1) recognizes the nucleolin <em>via</em> an aptamer AS1411, and meanwhile the i-motif in H1 undergoes structure switching at extracellular acid pH, enabling the cross-opening of H2, H3, H4, H5 for in situ self-assembly of two-sided DNAzyme nanoantennas. Upon internalization by cancer cells, DNAzymes in the nanoantenna cleave the substrates with intracellular Mg²⁺ as a cofactor, releasing abundant molecular beacons containing ASOs. ASOs hybridize with Egr-1 mRNAs, inducing the recovery of Cy5 fluorescence for Egr-1 mRNA imaging and silencing. This nanoantenna achieves a limit of detection of 7.46 fM for <em>in vitro</em> assay. It can profile Egr-1 mRNA level in diverse human cells, and distinguish Egr-1 mRNA level in breast cancer tissues and healthy counterparts. Moreover, it can be applied for high-contrast imaging of Egr-1 mRNA <em>in vivo</em>, efficient gene silencing, and enhanced tumor ablation, with promising applications in smart gene therapeutics and precise nanomedicines.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118016"},"PeriodicalIF":10.5,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145120424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoconfined metal-organic frameworks encapsulating platinum(II) complexes with self-catalytic aggregation-induced electrochemiluminescence for ultrasensitive miRNA-21 biosensing","authors":"Wanzhen Chen ,&nbsp;Ziqi Lian ,&nbsp;Rongkai Ye ,&nbsp;Jiaju Mo ,&nbsp;Yun Chen ,&nbsp;Ying Ma ,&nbsp;Jianqiang Hu ,&nbsp;Aiqing Li","doi":"10.1016/j.bios.2025.118019","DOIUrl":"10.1016/j.bios.2025.118019","url":null,"abstract":"<div><div>Aggregation-induced electrochemiluminescence (AIECL) luminophores have garnered significant attention for biosensing applications owing to their enhanced electrochemiluminescence (ECL) emission in aggregated states. However, conventional AIECL systems often suffer from structural instability in physiological environments due to weak intermolecular force of their aggregation. Here, we have successfully constructed nanoconfined Zr-based metal-organic frameworks (PCN-777 MOFs) encapsulating platinum(II)-[4,2':6′,4″-terpyridine]-4′-carboxylic acid (Pt(tpyc)²⁺) complexes, in which Pt(tpyc)²⁺) complexes are immobilized into PCN-777 MOFs <em>via</em> Zr⁴⁺ and -COO^- coordination bond. The resulting Pt-PCN-777 MOFs achieved exceptional structural stability and high loading efficiency of Pt(tpyc)²⁺. Critically, the mesoporous structural of the PCN-777-MOFs efficiently proconcentrates of S₂O₈²⁻ co-reactant within its channels, significantly shortening electron-transfer pathway between immobilized Pt(tpyc)²⁺ emitters and coreactants. The synergistic effect of the Pt-PCN-777 MOFs accelerates K₂S₂O₈ decomposition, leading to enhanced ECL signal. Through integrating catalytic hairpin assembly and hemin-quenching strategies, the \"Signal On-Off\" ECL biosensor is constructed for ultra-sensitive detection of miRNA-21. The miRNA-21 biosensor exhibits superior analytical performance, such as high sensitivity (approximately 9.03 × 10¹⁷), wide linear range (10²–10⁹ aM), femtomolar-level detection limit (∼66 aM), exceptional selectivity and operational stability. This study addresses the limitations of instability of traditional AIECL systems and is hopeful to establish a versatile platform of Pt(II)-MOF composites for advanced ECL probes in clinical diagnosis.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118019"},"PeriodicalIF":10.5,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145120704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Full-length spatial transcriptome strategy based on robust and low-cost target tissue capture","authors":"Ying Zhou ,&nbsp;Qinyu Ge ,&nbsp;Moxin Li ,&nbsp;Ting Qi ,&nbsp;Zhihui Li ,&nbsp;Yuqi Sheng ,&nbsp;Min Pan ,&nbsp;Quanjun Liu ,&nbsp;Xiangwei Zhao ,&nbsp;Zuhong Lu","doi":"10.1016/j.bios.2025.118018","DOIUrl":"10.1016/j.bios.2025.118018","url":null,"abstract":"<div><div>Smart-seq2, a widely used method for constructing full-length mRNA transcriptome libraries, can be combined with target tissue capture techniques such as Laser Capture Microdissection (LCM) to achieve robust spatial transcriptomic profiling. However, both Smart-seq2 and LCM are limited by high costs and complex operation, hindering their broader adoption. Here, we report a full-length spatial transcriptome strategy based on a robust and cost-effective tissue capture technique, termed MSN-seq. MSN-seq integrates microneedle-based sampling with a modified Smart-seq2 protocol. This method captures multi-cellular tissue spots and not only optimises Smart-seq2 for enhanced performance but also leverages reusable steel needles to further reduce costs and improve transcriptomic capture efficiency. In addition to simplifying experimental procedures, MSN-seq increases accessibility by enabling spatial transcriptomic analysis without requiring specialised skills or expensive instrumentation. We applied MSN-seq to brain and retinal tissues from models of Parkinson's disease and retinal degeneration, demonstrating its effectiveness in capturing spatial transcriptomic data. Furthermore, application of MSN-seq to frozen sections from U251 glioblastoma model mice revealed early invasive mechanisms and spatially restricted enrichment of immune cell subsets. Overall, MSN-seq provides a low-cost, easy-to-use, and highly efficient strategy for acquiring spatial transcriptome data.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118018"},"PeriodicalIF":10.5,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}