Biosensors and Bioelectronics最新文献

{"title":"Endoplasmic reticulum (ER)-targeted ratiometric fluorescent probe for visualization of ER-phagy induced by ONOO− and NIR imaging in mice with drug-induced liver injury","authors":"Peng Lei ,&nbsp;Ni Wu ,&nbsp;Zhi Yan ,&nbsp;Jisheng Nie ,&nbsp;Yating Meng ,&nbsp;Chuan Dong ,&nbsp;Shaomin Shuang ,&nbsp;Minglu Li","doi":"10.1016/j.bios.2025.117525","DOIUrl":"10.1016/j.bios.2025.117525","url":null,"abstract":"<div><div>Drug-induced liver injury (DILI) remains a persistent and unavoidable challenge in biomedicine, significantly impacting preclinical drug development and early clinical trials. The liver, as the primary site for endogenous antioxidant production, is particularly sensitive to acute damage mediated by reactive oxygen species (ROS) and reactive nitrogen species (RNS). The ratiometric and near-infrared (NIR) detection of peroxynitrite (ONOO⁻), as potential biomarker for DILI, is crucial for enhancing the imaging effectiveness of liver damage. Thus, an ONOO⁻ activated endoplasmic reticulum (ER)-targeted fluorescent probe, named DSA, was easily designed and synthesized using dicyanoisophorone, syringaldehyde and arylboronate, which featured dicyanoisophorone as the fluorophore and an arylboronate group as the ONOO⁻ recognition group. The probe DSA was converted into a long-wavelength DSA-ONOO^- fluorophore in the presence of ONOO⁻ with large Stokes shift (172 nm), high selectivity, and low detection limit (98 nM). The ratiometric response to ONOO⁻ provided a more accurate intracellular analysis through a built-in internal reference calibration, successfully enabling sensitive detection of ONOO⁻ in living cells. Additionally, leveraging the optical characteristics of NIR fluorescence imaging, DSA not only detected the ER-phagy process induced by starvation and ONOO⁻ but also accurately monitored minor fluctuations in ONOO⁻ levels during acetaminophen (APAP)-induced hepatotoxicity in mice, aiding in the diagnosis of DILI and the development of therapeutic drugs.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"283 ","pages":"Article 117525"},"PeriodicalIF":10.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzyme-free amplification-enhanced ratiometric fluorescence method for highly sensitive detection of acute myocardial infarction biomarkers","authors":"Jingjing Chen ,&nbsp;Xuexia Jia ,&nbsp;Ruipeng Chen ,&nbsp;Huanying Zhou ,&nbsp;Yu Wang ,&nbsp;Zhixian Gao","doi":"10.1016/j.bios.2025.117527","DOIUrl":"10.1016/j.bios.2025.117527","url":null,"abstract":"<div><div>A wide range of proteins, including enzymes, hormones, immune factors and regulatory proteins, are essential for a variety of physiological processes. Dysregulation of the levels of specific proteins in organisms is known to adversely affects the human body. Given the pathophysiological significance of cardiac troponin I (cTnI), simple methods for detecting the sensitivity and selectivity of cTnI in biological systems are in high demand and have therefore been extensively explored. Nonetheless, the early detection of low concentrations of cTnI in the human body continues to be a focal point and area of interest in bioassays, due to the low levels and intricate composition of biomarkers in bodily fluids (e.g., blood, sweat, urine, and saliva), which impose rigorous demands on the sensitivity and stability of detection methods. The assay platform is isothermal, homogeneous and has simple experimental conditions without proteases. Using ratiometric fluorescence with built-in self-correction, we achieved low background values and improved the accuracy of the assay results. Our method enhances detection sensitivity by utilizing the cycling of probe X during strand replacement. Triple sensitization was achieved by combining ratiometric fluorescence on the basis of normal strand displacement reaction. In the experiment, the dual sensitization was reflected by the high sensitivity (0.001 ng/mL) and wide linear detection range (0.001 ng/mL∼1000 ng/mL) of cTnI in real serum samples. In particular, the snapshots of the simulation process were recorded through accurate simulation calculations and multidimensional characterization analysis, which revealed the changes in the system energy during the simulation process. The sensing platform combines multiple technological advantages, and we are optimistic that the versatility of this research has great potential for application in the fields of early screening and diagnosis and military medical applications.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"283 ","pages":"Article 117527"},"PeriodicalIF":10.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143903364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A colorimetric biosensor based on 3D printed spiral mixer and magnetic continuous-flow separation for sensitive detection of Salmonella in centrifuge tubes","authors":"Siyi Ye,&nbsp;Kaiyuan Jia,&nbsp;Meixuan Li,&nbsp;Ying Ding,&nbsp;Gang Liu,&nbsp;Jianhan Lin,&nbsp;Yuhe Wang,&nbsp;Lei Wang","doi":"10.1016/j.bios.2025.117509","DOIUrl":"10.1016/j.bios.2025.117509","url":null,"abstract":"<div><div>Early screening of foodborne pathogens is effective to prevent food poisoning. In this work, a portable colorimetric biosensor was innovatively developed on a centrifugal tube using a 3D printed spiral mechanical mixer for rapid immune reaction, a 3D printed magnetic field generator for continuous-flow bacterial capture, Au@Pt nanozymes for effective signal amplification, and a smartphone application for accurate image processing. The rapid and efficient mixing of specific antibodies on the magnetic beads and Au@Pt nanozymes with target bacteria was simply achieved through repeated press-and-release actions on the mixer to produce the vortex turbulence in the sample chamber and thus form the magnetic bead-bacteria-nanozyme conjugates. The air pressure in the centrifuge tube was adjusted by manual operation on the disposable syringe. More importantly, the entire bacterial detection procedure from separation and labelling, then to washing and catalysis, and finally to measurement and analysis, was achieved on a single centrifugal tube. Besides, all the wastes were collected inside the tube to avoid cross-contamination risks. This disposable biosensor was featured with easy operation, low cost and high sensitivity, and was able to detect <em>Salmonella typhimurium</em> as low as 16 CFU/mL in 1 h, which might pave a pathway for on-site screening of foodborne pathogens to ensure food safety.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"282 ","pages":"Article 117509"},"PeriodicalIF":10.7,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143882881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microsampling and exosome-enriched optical biochip for non-invasive detection of breast cancer exosomes in clinical human tear fluid","authors":"Shuxin Zhang ,&nbsp;Jiamei Liu ,&nbsp;Mengmeng Li ,&nbsp;Yujia Zeng ,&nbsp;Tailin Xu","doi":"10.1016/j.bios.2025.117507","DOIUrl":"10.1016/j.bios.2025.117507","url":null,"abstract":"<div><div>Tumor exosome detection is one of the most clinically relevant liquid biopsy methods for non-invasive breast cancer diagnosis. However, conventional methods are often unsuitable for routine use due to large sample requirements, low precision, and complex procedures. To overcome these challenges, we have developed a novel optical biochip that integrates tear collection, exosome enrichment, and detection into a single platform. This biochip requires only 14 μL of tears and employs dual biomarker aptamers for the simultaneous identification and capture of target exosomes, forming sandwich-like complexes. Subsequently, the chip enriches these sandwich complexes through acoustic radiation, enabling separation from interferences and signal amplification. As a result, the biochip exhibits excellent specificity and ultra-high sensitivity for breast cancer cell exosomes, with a detection limit as low as 1.2 × 10²±0.16 × 10² particles/mL and a detection time of approximately 10.42 min. In addition, this optical biochip was used for the first time to detect exosomes related to breast cancer in tears, effectively distinguishing breast cancer patients from healthy donors with 100 % sensitivity and specificity, offering a meaningful approach for on - site breast cancer diagnosis and personalized medical health.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"282 ","pages":"Article 117507"},"PeriodicalIF":10.7,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Core-shell structured gold nanoparticle-AIEgen nanohybrids for enhanced dual-mode lateral flow immunoassay of fumonisin B1","authors":"Yi Zhang ,&nbsp;Ge Xu ,&nbsp;Xirui Chen ,&nbsp;Zhiwen Hu ,&nbsp;Lu Ding ,&nbsp;Xiaolin Huang","doi":"10.1016/j.bios.2025.117508","DOIUrl":"10.1016/j.bios.2025.117508","url":null,"abstract":"<div><div>Herein, we report the design and synthesis of bifunctional colorimetric and fluorescent nanohybrids (Au-AIENPs) through the co-assembly of oleylamine-modified gold nanoparticles (OA-AuNPs) and aggregation-induced emission luminogens (AIEgen). Due to the distinct compatibility of the components, the resulting Au-AIENPs form a core-shell nanostructure, with the AIEgen creating a fluorescent core and the OA-AuNPs uniformly dispersing on the shell. This spatial separation of AIEgen and OA-AuNPs optimizes the colorimetric signal while preserving a strong fluorescence output. Leveraging the intrinsic dual functionality of Au-AIENPs as dual-mode signal reporters, we demonstrate their potential for rapid, naked-eye visualization and fluorescent quantitative detection of fumonisin B1 (FB1) on the lateral flow immunoassay (LFIA) platform (Au-AIENPs-LFIA). Under optimal conditions, the developed Au-AIENPs-LFIA test strip achieved high sensitivity for FB1 qualitative detection, with a visual limit of detection (LOD) of 0.056 ng/mL, and exhibited ultrasensitivity for FB1 quantitative detection, with an LOD of 0.03 ng/mL. In conclusion, the proposed Au-AIENPs offer an ideal combination of visual compatibility and high sensitivity, providing significant practical implications for expanding LFIA applications, particularly in resource-limited settings.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"282 ","pages":"Article 117508"},"PeriodicalIF":10.7,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143882880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nucleic acid-mediated SERS Biosensors: Signal enhancement strategies and applications","authors":"Yushi Xie ,&nbsp;Jiaqiang Huang ,&nbsp;Min Yang ,&nbsp;Yangzi Zhang ,&nbsp;Xiaobo Zhang ,&nbsp;Wentao Xu ,&nbsp;Jijuan Cao ,&nbsp;Longjiao Zhu","doi":"10.1016/j.bios.2025.117519","DOIUrl":"10.1016/j.bios.2025.117519","url":null,"abstract":"<div><div>Surface Enhanced Raman Spectroscopy (SERS) is a powerful spectroscopic analysis technique applied in various fields due to its high selectivity, ultra-high sensitivity, and non-destructiveness. As natural biological macromolecules, nucleic acids perform a significant role in SERS biosensing. In this review, we first summarize how nucleic acids mediate the signal enhancement of SERS biosensors from three aspects: substrate self-assembly, analyte biorecognition, and molecular amplification. Among them, SERS substrates can be self-assembled by both DNA modification and coordination or electrostatic interactions. In the field of biorecognition, analyte biorecognition based on three nucleic acid recognition elements can enhance SERS signals by regulating the distance of analytes or Raman reporter molecules to the SERS substrate. In addition, nucleic acid-based enzyme and enzyme-free amplification can enhance SERS signals by enlarging the quantity of analytes or its nucleic acid intermediates. Subsequently, multidimensional applications of nucleic acid-mediated SERS signal enhancement in biomedicine, food safety, and environmental monitoring are listed. Finally, the current challenges and future exploration of nucleic acid-mediated SERS signal enhancement are discussed.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"282 ","pages":"Article 117519"},"PeriodicalIF":10.7,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143882879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From lab to clinic: A portable and automated microfluidic nucleic acid detection instrument for rapid STI pathogen identification","authors":"Shutao Liu ,&nbsp;Feifei Zhou ,&nbsp;Kailin Li ,&nbsp;Sunyi Chen ,&nbsp;Binfen Hu ,&nbsp;Yixuan Feng ,&nbsp;Hao Song ,&nbsp;Xiaonian Lu ,&nbsp;Dou Wang ,&nbsp;Chenchen Ge","doi":"10.1016/j.bios.2025.117498","DOIUrl":"10.1016/j.bios.2025.117498","url":null,"abstract":"<div><div>Sexually transmitted infections (STIs) remain a significant global public health challenge. The rapid and precise identification of these pathogens is crucial for effective diagnosis and treatment. Current polymerase chain reaction (PCR)-based assays, despite their widespread use, present several limitations in clinical settings, including high costs and technical complexity. To address these challenges, we developed a portable and automated microfluidic nucleic acid detection device for multiplex detection of three sexually transmitted pathogens via the fluorescence quantitative PCR (qPCR) assay. This integrated platform combines automated sample processing, multiplex qPCR amplification, and fluorescence detection within a single device. The entire closed-loop testing procedure, from automated nucleic acid extraction to result generation, requires only 40 min. The system demonstrates detection limits of 360 copies/ml for Neisseria gonorrhoeae (NG), 140 copies/ml for Ureaplasma urealyticum (UU), and 290 copies/ml for Chlamydia trachomatis (CT). Clinical validation revealed exceptional performance in multiplex detection of infected specimens, showing complete concordance (100 %) with standard diagnostic results while maintaining high specificity. These findings highlight the instrument's clinical potential and establish a new paradigm for automated, multiplex pathogen detection.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"282 ","pages":"Article 117498"},"PeriodicalIF":10.7,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143879028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling a novel nanozyme cofactor: a highly activated Fe-CDs-derived colorimetric sensor array for comprehensive authentication of diverse tea products","authors":"Yanying Zheng ,&nbsp;Wenju Yan ,&nbsp;Nansheng Li ,&nbsp;Jinfang Nie ,&nbsp;Yun Zhang ,&nbsp;Wenxin Niu ,&nbsp;Yali Yuan","doi":"10.1016/j.bios.2025.117510","DOIUrl":"10.1016/j.bios.2025.117510","url":null,"abstract":"<div><div>Tea has antioxidant, anti-aging, hypotensive, and other functions. The tea polyphenol profile is of great significance for the identification and quality control of diverse tea products. Herein, an iron-doped carbon dots (Fe-CDs) with peroxidase-like activity was synthesized to construct a colorimetric sensor array for the tea polyphenols analysis in a complex matrix. Unexpectedly, it is found that the addition of tea polyphenols leads to an admirable increase in the activity of Fe-CDs, indicating the role of tea polyphenols as a cofactor of Fe-CDs nanozyme. The exploration based on multiple techniques reveals that the polyphenols can absorb on the Fe-CDs surface to form the new complex and effectively enhance the catalytic performance of Fe-CDs. This enhancement effect is closely related to the elemental doping, surface condition of nanozyme, and the polyphenol concentration. Taking TMB as the substrate, the colorimetric fingerprint generated in the presence of different tea polyphenols are extracted from the sensing array. The results of principal component analysis (PCA) and hierarchical clustering analysis (HCA) show that five tea polyphenols of different types, concentrations, and tea polyphenol mixtures of different proportions are successfully distinguished through pattern recognition methods. Moreover, the identification of tea leaves from different years and types, as well as various tea drinks, has been further realized. This study not only paves a new direction for enhancing the nanozyme activity, but provides an effective and convenient strategy for the quality control of tea products.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"282 ","pages":"Article 117510"},"PeriodicalIF":10.7,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel 6xHis/HaloTag mammalian expressed autoantigens for the detection of humoral response with multiplexed electrochemical biosensors: A breakthrough in colorectal cancer and Alzheimer's disease personalized diagnostics","authors":"Eloy Povedano ,&nbsp;María Garranzo-Asensio ,&nbsp;Ana Montero-Calle ,&nbsp;Alejandro Valverde ,&nbsp;Pablo Dalmasso ,&nbsp;Pablo San Segundo-Acosta ,&nbsp;Olga Cano ,&nbsp;Mónica Vázquez ,&nbsp;Vicente Mas ,&nbsp;María Jesús Fernández-Aceñero ,&nbsp;Gustavo Rivas ,&nbsp;José M. Pingarrón ,&nbsp;Susana Campuzano ,&nbsp;Rodrigo Barderas","doi":"10.1016/j.bios.2025.117506","DOIUrl":"10.1016/j.bios.2025.117506","url":null,"abstract":"<div><div>The integration of autoantibody detection with electrochemical biosensors allows the development of a novel strategy for managing colorectal cancer (CRC) and Alzheimer's disease (AD). This work reports the use, for the first time, of sustainable receptors, autoantigens expressed in mammalian cells, fused to 6xHis at the N-terminus and HaloTag at the C-terminus, immobilized on chloroalkane-modified magnetic beads (MBs) to selectively capture plasma autoantibodies. Detection was based on amperometric measurements using disposable multiplexed screen-printed carbon electrodes (x8 sensing surfaces), horseradish peroxidase (HRP)-conjugated secondary antibodies, and the HQ/H₂O₂ system. HaloTag immobilization gave rise to a great sensitivity allowing discrimination between patients and healthy individuals in comparison with HisTag or -COOH immobilization. The CRC biosensor integrated seven CRC-specific full-length autoantigens, while the AD bioplatform combined three AD-specific full-length proteins and four AD-specific peptides, which allowed a robust diagnostic performance validated by ROC analyses. These multiplexed biosensors provide minimally invasive, cost-effective, and sustainable alternatives to traditional diagnostic methods, and show ideal for discovery of new targets and for mass screening and early disease detection, supporting personalized medicine approaches.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"282 ","pages":"Article 117506"},"PeriodicalIF":10.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An electrochemiluminescence sensor with wide stable potential detection window based on superhydrophilic amino acid polyionic liquid for the detection of miRNA-128 in colorectal cancer","authors":"Yunxue Zhu,&nbsp;Zihui Liang,&nbsp;Peilin Wang,&nbsp;Qiang Ma,&nbsp;Zhiquan Zhang","doi":"10.1016/j.bios.2025.117502","DOIUrl":"10.1016/j.bios.2025.117502","url":null,"abstract":"<div><div>The stable potential detection window (PW) of electrodes in the commonly overlooked issue in electrochemiluminescence (ECL) research. In this study, an amino acid-based polyionic liquid (AAPIL) had been designed and synthesized to solve the problem of narrow potential detection window of the electrode. A biosensor was constructed based on the AAPIL to overcome the limitation and enhance ECL signals. The reduced titanium nanoclusters (R-Ti NCs) were used as luminescent probes with strong ECL signal. Moreover, AAPIL with high ionic conductivity effectively accelerated electron transfer and facilitated the luminescence of titanium nanoclusters. In addition, the superhydrophilicity AAPIL had good antifouling properties. Therefore, the AAPIL-based biosensor was used to quantify miRNA-128 with a linear detection range of 1 fM to 1 nM, while the detection limit was as low as 0.17 fM. Owing to its excellent electrochemical property, this ECL sensor can be successfully applied to the detection of actual colorectal cancer samples.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"282 ","pages":"Article 117502"},"PeriodicalIF":10.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143868821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}