Biosensors and Bioelectronics最新文献_第5页

A turn-on CRISPR/Cas12a strategy featuring a sterically-hindered activator for in situ fluorescence imaging of H2O2 in vivo 一种具有位阻激活剂的CRISPR/Cas12a策略，用于体内H2O2的原位荧光成像。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-05-15 Epub Date: 2026-01-30 DOI: 10.1016/j.bios.2026.118449

Zhiqiang Li , Wenxian Zhang , Zhe Feng , Zheng Liu , Zhiyuan Feng , Yang Shi , Jiayin Zhan , Jingjing Zhang

{"title":"A turn-on CRISPR/Cas12a strategy featuring a sterically-hindered activator for in situ fluorescence imaging of H2O2 in vivo","authors":"Zhiqiang Li , Wenxian Zhang , Zhe Feng , Zheng Liu , Zhiyuan Feng , Yang Shi , Jiayin Zhan , Jingjing Zhang","doi":"10.1016/j.bios.2026.118449","DOIUrl":"10.1016/j.bios.2026.118449","url":null,"abstract":"<div><div>Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) serves as a key biomarker of oxidative stress in pathological processes such as cancer and inflammation. However, its in vivo visualization remains challenging due to the lack of sensitive, rapid, and bioorthogonal imaging methods. Here, we present a H<sub>2</sub>O<sub>2</sub>-activatable CRISPR/Cas12a strategy, termed A-BO-CRISPR, for real-time fluorescence imaging in living systems. This biosensing strategy employs a 4-bromomethylphenylboronic acid pinacol ester-caged DNA activator whose binding to crRNA is initially blocked by steric hindrance, effectively suppressing Cas12a trans-cleavage activity. Upon encountering endogenous H<sub>2</sub>O<sub>2</sub>, the boronate ester is selectively hydrolyzed, restoring activator/crRNA hybridization and triggering amplified fluorescent signal generation via Cas12a-mediated collateral cleavage of a ssDNA reporter. The system achieves a detection limit of 0.64 μM and responds within minutes, enabling real-time monitoring of H<sub>2</sub>O<sub>2</sub> fluxes in living cells and tumor-bearing mice. It exhibits high selectivity and robust stability in complex biological environments. By integrating a chemical gating mechanism with CRISPR-based signal amplification, this work paves the way for potential applications in probing redox biology, imaging-guided diagnostics and therapeutic monitoring.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"300 ","pages":"Article 118449"},"PeriodicalIF":10.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146117103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Active detection of melanoma via a dual-extraction-driven flexible microneedles sensor 通过双萃取驱动的柔性微针传感器主动检测黑色素瘤。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-05-15 Epub Date: 2026-01-28 DOI: 10.1016/j.bios.2026.118451

Min Wu , Songlong Jiao , Lei Liu

{"title":"Active detection of melanoma via a dual-extraction-driven flexible microneedles sensor","authors":"Min Wu , Songlong Jiao , Lei Liu","doi":"10.1016/j.bios.2026.118451","DOIUrl":"10.1016/j.bios.2026.118451","url":null,"abstract":"<div><div>Wearable microneedles (MNs) biosensors offer significant potential for minimally invasive biomarker detection in interstitial fluid (ISF). However, suboptimal fluid collection efficiency, dermal irritation due to rigid structural components, and inadequate long-term operational stability collectively represent critical barriers to their clinical translation for early disease diagnosis. Here, we propose a fully integrated dual-extraction-driven flexible microneedles (DFMN) sensor for active detection of melanoma-associated miRNA. The DFMN sensor comprises hydrogel MNs, hollow Au-Ag NPs functionalized flexible electrodes, and a miniaturized wireless module. The synergistic combination of passive diffusion driven by hydrogel MN swelling and active transport <em>via</em> reverse iontophoresis enhanced ISF extraction volume by 1.6-fold within 5 min. This dual-extraction mechanism enabled real-time wireless detection of miRNA-221 with an ultra-low limit of detection (LOD) of 43 aM. Furthermore, to enhance reliability in complex biological environments, an artificial neural network algorithm was implemented, achieving a correlation coefficient (R<sup>2</sup>) of 0.985. The DFMN sensor holds broad application prospects in interdisciplinary fields such as human-machine interaction, smart healthcare, and artificial intelligence, providing an innovative solution for real-time sensitive detection of wearable flexible sensors.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"300 ","pages":"Article 118451"},"PeriodicalIF":10.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146123043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Open-surface digital ELISA enabled by magnetic trapping and deep learning for rapid and highly sensitive detection of African swine fever 开放表面数字ELISA通过磁捕获和深度学习实现快速和高灵敏度检测非洲猪瘟。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-05-15 Epub Date: 2026-02-02 DOI: 10.1016/j.bios.2026.118473

Yonghong Zha , Shufei Liao , Yazhi Li , Yibo Wang , Haonan Li , Jiaqi Wang , Yiren Liu , Jianpei Dong , Mingxia Sun , Jianhua Zhou

{"title":"Open-surface digital ELISA enabled by magnetic trapping and deep learning for rapid and highly sensitive detection of African swine fever","authors":"Yonghong Zha , Shufei Liao , Yazhi Li , Yibo Wang , Haonan Li , Jiaqi Wang , Yiren Liu , Jianpei Dong , Mingxia Sun , Jianhua Zhou","doi":"10.1016/j.bios.2026.118473","DOIUrl":"10.1016/j.bios.2026.118473","url":null,"abstract":"<div><div>African swine fever (ASF), a highly fatal and contagious animal disease caused by ASF virus, leads to substantial economic losses. Early detection is critical, as the viral load is initially low, demanding highly sensitive detection of the viral p30 antigen. Although the existing digital ELISA enables sensitive detection of p30, its performance is constrained by inefficient microcavity utilization (resulting in Poisson noise-limited sensitivity) and low image recognition accuracy. This study presents an open-surface digital ELISA (OS-dELISA) platform that integrates an open-space magnetic bead (MB) array with artificial intelligence. The platform uses magnetic trapping to form the MB array, which acts as an open-space microcavity. Combined with reciprocating-flow microfluidics and in-situ tyramine signal amplification, this design eliminates the additional step of encapsulating immunocomplexes into enclosed microcavities, enhances microcavity utilization and antigen capture efficiency, and improves sensitivity. An improved mask region-based convolutional neural network enables intelligent and accurate image recognition for large-scale datasets. OS-dELISA achieves highly sensitive p30 detection within 17 min, with a detection limit of 21 fg/mL. Serum samples validation showed 100% sensitivity and 95% specificity. By combining microfluidics and artificial intelligence, OS-dELISA provides a powerful tool for intelligent animal disease diagnostics.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"300 ","pages":"Article 118473"},"PeriodicalIF":10.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146130408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wearable multifunctional microfluidic pressure sensor based on activated laser-induced biochar nanofluid for human health monitoring applications 基于活化激光诱导生物炭纳米流体的可穿戴多功能微流体压力传感器用于人体健康监测。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-05-15 Epub Date: 2026-01-29 DOI: 10.1016/j.bios.2026.118462

Mir Mehdi Hashemi , Farzane Hasheminia , Amirhossein Heydariyan , Sadegh Sadeghzadeh

{"title":"Wearable multifunctional microfluidic pressure sensor based on activated laser-induced biochar nanofluid for human health monitoring applications","authors":"Mir Mehdi Hashemi , Farzane Hasheminia , Amirhossein Heydariyan , Sadegh Sadeghzadeh","doi":"10.1016/j.bios.2026.118462","DOIUrl":"10.1016/j.bios.2026.118462","url":null,"abstract":"<div><div>This study reports the development of Activated Biochar Carbon Nanoparticles (ABCN) from rice husk waste via CO<sub>2</sub> laser ablation, acid washing, and thermal activation, creating a porous, conductive nanomaterial. Comprehensive characterization using SEM, DLS, XRD, and FTIR confirmed ABCN's structural properties, with derived nanofluids showing 71 nm average particle size and excellent colloidal stability in DI water. The nanofluid was integrated into a microfluidic pressure sensor using soft lithography, demonstrating piezo-resistive functionality across 337 Pa to 60 kPa with strong linearity/repeatability. The sensor achieved rapid response (∼100ms) and recovery (∼70ms) times, enabling real-time monitoring of physiological signals (pulse waves, respiration) and acoustic discrimination of spoken words. This work establishes ABCN-based microfluidic sensors as promising wearable platforms for healthcare diagnostics, combining sustainable materials with high-performance sensing capabilities. The sensor's multifunctionality, derived from agricultural waste, addresses critical needs for affordable, non-invasive health monitoring solutions while demonstrating novel applications in both physiological and acoustic signal detection. These findings highlight the potential of laser-activated biochar nanomaterials for next-generation biomedical devices.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"300 ","pages":"Article 118462"},"PeriodicalIF":10.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146117050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered DNA hydrogel paper chip biosensor by cascaded hybridization chain reaction-assisted CRISPR/Cas12a system for sensitive detection of miRNA 622 利用级联杂交链反应辅助CRISPR/Cas12a系统设计的DNA水凝胶纸芯片生物传感器，用于miRNA 622的灵敏检测。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-05-15 Epub Date: 2026-02-02 DOI: 10.1016/j.bios.2026.118482

Shujing Wang , Chengxiang Li , Longfei Zhu , Kaiyue Liu , Liyuan Jing , Li Xie , Haipeng Guo , Shenguang Ge , Jinghua Yu

{"title":"Engineered DNA hydrogel paper chip biosensor by cascaded hybridization chain reaction-assisted CRISPR/Cas12a system for sensitive detection of miRNA 622","authors":"Shujing Wang , Chengxiang Li , Longfei Zhu , Kaiyue Liu , Liyuan Jing , Li Xie , Haipeng Guo , Shenguang Ge , Jinghua Yu","doi":"10.1016/j.bios.2026.118482","DOIUrl":"10.1016/j.bios.2026.118482","url":null,"abstract":"<div><div>The integration of DNA hydrogel with electrochemiluminescence (ECL) technology represents a synergistic enhancement through molecular-level precision design and nanoscale coordination. This strategic integration confers biosensors novel functionalities including intelligent responsiveness and environmental adaptability. In this study, a cascaded hybridization chain reaction (HCR) and CRISPR/Cas12a-integrated DNA hydrogel paper chip was engineered for ultrasensitive microRNA 622 (miRNA 622) detection. Target miRNA 622 triggered HCR amplification via hairpin DNA assembly, while Cas12a recognized protospacer adjacent motif (PAM) sequences within the HCR-generated double-stranded products to activate its <em>trans</em>-cleavage ability. The DNA hydrogel was constructed through copolymerization of acrylamide-modified DNA single strands (SA and SB) with Ru (II) complex-functionalized linker DNA. Activated Cas12a cleaved single-stranded DNA within the DNA hydrogel network, thereby releasing Ru (II) complexes. AuPd nanoparticles (AuPd NPs) served as the co-reactant accelerator, amplifying the cathodic ECL signals of the liberated Ru (II) complexes. The developed platform demonstrated a dynamic detection range from 0.001 to 500 pM with a detection limit of 0.33 fM, establishing a groundbreaking approach for detecting miRNA 622 in clinical diagnostics.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"300 ","pages":"Article 118482"},"PeriodicalIF":10.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146123009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A FEN1-EDCR dual-amplification strategy for ultrasensitive detection of CHCHD10 c.176C>T mutation FEN1-EDCR双扩增策略超灵敏检测CHCHD10 c.176C>T突变

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-05-15 Epub Date: 2026-01-31 DOI: 10.1016/j.bios.2026.118468

Han Pan , Murilege Chao , Miao He , Jinhua Chen , Feifan Yin , Yongliang Cai , Wenting Cheng , Zhongyun Wang , Yang Xiang

{"title":"A FEN1-EDCR dual-amplification strategy for ultrasensitive detection of CHCHD10 c.176C>T mutation","authors":"Han Pan , Murilege Chao , Miao He , Jinhua Chen , Feifan Yin , Yongliang Cai , Wenting Cheng , Zhongyun Wang , Yang Xiang","doi":"10.1016/j.bios.2026.118468","DOIUrl":"10.1016/j.bios.2026.118468","url":null,"abstract":"<div><div>A single-base mutation in the <em>CHCHD10</em> gene can impair thermogenic responses, and inhibit the efficient conversion of white adipose tissue to beige adipose tissue. However, achieving high-fidelity detection of such low-abundance single nucleotide polymorphisms (SNPs) within complex biological matrices remains a critical technological challenge. This study developed a novel method for detecting a single-base mutation in <em>CHCHD1</em>0 by integrating the specific recognition of flap endonuclease 1 (FEN1) with the cascade amplification of an entropy-driven circuit reaction (EDCR), supplemented with magnetic separation to suppress background interference. In this process, the mutant target (MT) hybridizes with the upstream probe (UP) and a hairpin downstream probe (DP) to form a specific three-base overlapping structure. FEN1 recognizes and cleaves this structure, releasing a cleavage sequence (CS). Following magnetic purification, the CS activates the EDCR, enabling dual-signal amplification. The hairpin DP design effectively conceals the CS, significantly reducing background noise. The method achieves a detection limit of 0.23 pM, discriminates mutation abundances as low as 0.01% in mixed DNA samples, and demonstrates robust performance in complex matrices such as human serum and high-background genomic DNA. This work provides an innovative and reliable methodological tool for the detection of low-abundance point mutations in complex samples.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"300 ","pages":"Article 118468"},"PeriodicalIF":10.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146098712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A three-dimensional skin-conformal hybrid electrode system for burden-free sEMG monitoring during exercises 用于运动中无负荷肌电监测的三维皮肤-适形混合电极系统。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-05-15 Epub Date: 2026-02-06 DOI: 10.1016/j.bios.2026.118502

Yichuan Mao , Dingyu Nie , Hongwei Sheng, Peiming Lei, Jinkun Hu, Huasheng Bi, Qing Yue, Wei Lan

{"title":"A three-dimensional skin-conformal hybrid electrode system for burden-free sEMG monitoring during exercises","authors":"Yichuan Mao , Dingyu Nie , Hongwei Sheng, Peiming Lei, Jinkun Hu, Huasheng Bi, Qing Yue, Wei Lan","doi":"10.1016/j.bios.2026.118502","DOIUrl":"10.1016/j.bios.2026.118502","url":null,"abstract":"<div><div>High-fidelity surface electromyography (sEMG) acquisition under dynamic conditions is critical for rehabilitation, sports monitoring, and human-machine interaction. Conventional electrodes often suffer from poor compliance, weak adhesion, and unstable skin-electrode interfaces during motion, degrading signal quality. In this paper, we propose a three-dimensional hybrid electrode system (THES) that synergistically integrates a thermo-responsive sponge-gel electrode with a stretchable electronic architecture and wireless sEMG acquisition. The electrode achieves rapid body-temperature-triggered adhesion, high stretchability, and low impedance, enabling conformal contact and stress absorption. Simulations and experiments confirm that the three-dimensional (3D) structure mitigates stress concentration and maintains conductivity under deformation, thereby stabilizing the skin-electrode interface during dynamic motion. The system records sEMG signals at a high sampling rate, supporting muscle force estimation, fatigue monitoring, and motion recognition. In badminton scenarios, THES enables stable on-skin sEMG acquisition during dynamic movements, supporting the recording of distinct muscle activation patterns across different stroke postures as well as long-duration exercise. Leveraging these high-quality signals, our dual vision transformer (DualViT) framework, which fuses dual-modal features, classifies eight stroke actions with an accuracy of 99.34%, outperforming conventional models. By synergistically optimizing materials, structures, and algorithms, THES advances sEMG recording under dynamic conditions, offering a viable platform for wearable bioelectronic applications.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"300 ","pages":"Article 118502"},"PeriodicalIF":10.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146148602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A multimodal stretchable bioelectronic system for simultaneous monitoring of skeletal-muscular and metabolic biomarkers 一种多模态可拉伸生物电子系统，用于同时监测骨骼-肌肉和代谢生物标志物。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-05-15 Epub Date: 2026-02-05 DOI: 10.1016/j.bios.2026.118474

Jian Cai, Chong-Bo Ma, Jing Bai, Xiangjie Bo, Ming Zhou

{"title":"A multimodal stretchable bioelectronic system for simultaneous monitoring of skeletal-muscular and metabolic biomarkers","authors":"Jian Cai, Chong-Bo Ma, Jing Bai, Xiangjie Bo, Ming Zhou","doi":"10.1016/j.bios.2026.118474","DOIUrl":"10.1016/j.bios.2026.118474","url":null,"abstract":"<div><div>Physical activity involves coordinated skeletal muscle contraction and metabolic regulation, with its impact on human health determined by complex interactions between mechanical motion and biochemical processes. Monitoring these interactions requires real-time tracking of skeletal muscle activity that regulates movement and the associated metabolites produced during exercise. Here, we present iTrainer, a stand-alone, multimodal, stretchable, and wearable bioelectronic system that enables non-invasive and dynamic monitoring of skeletal-muscular and metabolic biomarkers. The device simultaneously captures physiological signals of skeletal muscle (motion frequency, skin strain, joint bending angle, and temperature) and sweat-based metabolic biomarkers (lactate and Na<sup>+</sup>) during both aerobic and anaerobic exercise. The iTrainer integrates three key components: an epidermal microfluidic module for skin-worn sweat collection and transport, a multimodal sensing array module for skin-on physicochemical biomarkers monitoring, and an integrated electronic module for data processing and wireless transmission. Human trials in aerobic (e.g., running) and anaerobic (e.g., bench press) exercise validated the system's capability to measure synchronized mechanical and biochemical signals. We also evaluate the utility of iTrainer as a partial substitute for professional coaches in the iTrainer-assisted self-exercise training management for novice individuals.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"300 ","pages":"Article 118474"},"PeriodicalIF":10.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146140519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA-engineered immunosensing platform for ultrasensitive detection of clinical protein biomarkers 用于临床蛋白生物标志物超灵敏检测的dna工程免疫传感平台。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-05-15 Epub Date: 2026-02-04 DOI: 10.1016/j.bios.2026.118485

Wenhao Zhang , Wenzhi Yang , Junru Li , Peipei Zhao , Weiqiang Yang , Shan Cao , Yongming Han , Xiaohan Cai , Siqi Tian , Jinxinyi Xu , Huili Dai , Dayong Yang , Fengqin Li , Peifeng Liu

{"title":"DNA-engineered immunosensing platform for ultrasensitive detection of clinical protein biomarkers","authors":"Wenhao Zhang , Wenzhi Yang , Junru Li , Peipei Zhao , Weiqiang Yang , Shan Cao , Yongming Han , Xiaohan Cai , Siqi Tian , Jinxinyi Xu , Huili Dai , Dayong Yang , Fengqin Li , Peifeng Liu","doi":"10.1016/j.bios.2026.118485","DOIUrl":"10.1016/j.bios.2026.118485","url":null,"abstract":"<div><div>Detection of protein biomarkers is crucial for early diagnosis, dynamic monitoring, and prognosis evaluation of various diseases. However, the surface immobilization methods of capture probes directly affect the detection performance of immunosensors. Herein, a DNA-engineered immunosensing platform (DEISP) for ultrasensitive detection of clinical protein biomarkers is presented, which employs DNA duplexes to immobilize capture antibodies on the sensing interface. This DNA-engineered immobilization approach not only firmly anchors the capture antibody with excellent biocompatibility, but also preserves its binding sites for target molecules. As proof of concept, DEISP was employed to detect the kidney injury biomarker neutrophil gelatinase-associated lipocalin (NGAL), the pro-inflammatory cytokine interleukin-6 (IL-6), and the cardiac troponin I (cTnI) associated with myocardial infarction, demonstrating remarkable sensitivity with limits of detection (LODs) of 0.025 pg mL<sup>−1</sup> for NGAL, 0.026 pg mL<sup>−1</sup> for IL-6, and 2.35 pg mL<sup>−1</sup> for cTnI, as well as a dynamic range spanning 7 orders of magnitude. Moreover, DEISP exhibits 91% accuracy in clinical serum and urine samples and a total assay time of 90 min. Furthermore, DEISP significantly minimizes sample volume and simplifies operational procedures while achieving ultrahigh sensitivity, highlighting its potential for point-of-care testing (POCT), multi-marker screening, and precision medicine.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"300 ","pages":"Article 118485"},"PeriodicalIF":10.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146140540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intracellular oxygen measurement in vivo by smartphone readout of endogenous Protoporphyrin IX delayed fluorescence 通过智能手机读出内源性原卟啉IX延迟荧光测定体内细胞内氧。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-05-15 Epub Date: 2026-02-01 DOI: 10.1016/j.bios.2026.118472

Protik Chandra Biswas , Jason R. Gunn , Tayyaba Hasan , Brian W. Pogue

{"title":"Intracellular oxygen measurement in vivo by smartphone readout of endogenous Protoporphyrin IX delayed fluorescence","authors":"Protik Chandra Biswas , Jason R. Gunn , Tayyaba Hasan , Brian W. Pogue","doi":"10.1016/j.bios.2026.118472","DOIUrl":"10.1016/j.bios.2026.118472","url":null,"abstract":"<div><div>Direct measurement of mitochondrial oxygen tension in vivo provides direct information on tissue metabolism and could facilitate new approaches in disease detection, function monitoring, and treatment efficacy assessment with cellular level data, with far superior sensitivity to oxygen changes than blood saturation measures. Here, a platform system was engineered to quantify fast sampling of oxygen partial pressure (pO<sub>2</sub>) inside tissue by utilizing the inherent rolling shutter readout of a smartphone CMOS camera detector for measuring the oxygen-sensitive time-resolved delayed fluorescence (DF) signal from Protoporphyrin IX (PpIX) which naturally occur in mitochondria for most tissues. The CMOS rolling shutter readout produces a microsecond-level time difference in the pixels row-by-row detection of light, here utilized as a time-gated shutter to sample the time distributed DF intensity. This novel technique eliminated the necessity of high-speed intensified camera with excitation isolation facility as well as advanced precise time synchronization system as required in the conventional time-resolved fluorescence lifetime measurement platforms for quantifying time-dependent very low intensity DF distribution of PpIX conjugated with prompt fluorescence. Both steady state and dynamic performance of the instrument were validated in tissue phantoms at different PpIX concentrations (0.5–10 μM) for wide range of pO<sub>2</sub> detection (0–160 mmHg) with tunable fast response time (1–3.5 s) which is substantially faster than electrode-based systems that measure over 10's of seconds. Finally, it was tested in vivo to assess the impact of dynamic inhaled oxygen concentration variation on skin tissue pO<sub>2</sub> under the conditions of normoxia, hyperoxia and hypoxia.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"300 ","pages":"Article 118472"},"PeriodicalIF":10.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146123014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0