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Dendrimer-based DNA biosensor for HIV virus detection 用于HIV病毒检测的树突DNA生物传感器。
IF 10.5 1区 生物学
Biosensors and Bioelectronics Pub Date : 2025-09-26 DOI: 10.1016/j.bios.2025.118014
Estefanía Enebral Romero , Marta Toldos-Torres , David López-Diego , Mónica Luna , Marta Failde , Brais González-Tobío , Félix Zamora , Iker Falces-Romero , María Luisa Montes , Tania García-Mendiola
{"title":"Dendrimer-based DNA biosensor for HIV virus detection","authors":"Estefanía Enebral Romero ,&nbsp;Marta Toldos-Torres ,&nbsp;David López-Diego ,&nbsp;Mónica Luna ,&nbsp;Marta Failde ,&nbsp;Brais González-Tobío ,&nbsp;Félix Zamora ,&nbsp;Iker Falces-Romero ,&nbsp;María Luisa Montes ,&nbsp;Tania García-Mendiola","doi":"10.1016/j.bios.2025.118014","DOIUrl":"10.1016/j.bios.2025.118014","url":null,"abstract":"<div><div>In this work, we propose an innovative electrochemical DNA biosensor for the early, rapid, selective, and sensitive detection of the human immunodeficiency virus (HIV) based on its genetic code. This platform is based on the integration of few-layer bismuthene (FLB) which provides a nanostructured surface and facilitates the anchoring of the DNA dendrimer which incorporates the biorecognition element (a capture probe complementary to the HIV-specific DNA sequence). The hybridization between the capture probe and the specific HIV target sequence is detected using Azure A (AA) as a redox indicator. The dendrimer-based DNA biosensor selectively detects HIV from 10.0 fM to 10.0 pM, exhibit a low detection limit of 3.03 fM and a 60-day stability, and has been validated using plasma samples from infected patients with varying viral loads.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 118014"},"PeriodicalIF":10.5,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synthetic biology-driven biosensors for healthcare applications: A roadmap toward programmable and intelligent diagnostics 用于医疗保健应用的合成生物学驱动的生物传感器:迈向可编程和智能诊断的路线图。
IF 10.5 1区 生物学
Biosensors and Bioelectronics Pub Date : 2025-09-26 DOI: 10.1016/j.bios.2025.118036
Begüm Sarac , Seydanur Yücer , Fatih Ciftci
{"title":"Synthetic biology-driven biosensors for healthcare applications: A roadmap toward programmable and intelligent diagnostics","authors":"Begüm Sarac ,&nbsp;Seydanur Yücer ,&nbsp;Fatih Ciftci","doi":"10.1016/j.bios.2025.118036","DOIUrl":"10.1016/j.bios.2025.118036","url":null,"abstract":"<div><div>Synthetic biology has revolutionized biosensor design by enabling programmable, modular systems that integrate biological components with engineered logic. This review explores recent innovations in synthetic biology-driven biosensors, highlighting applications in healthcare, environmental monitoring, and point-of-care diagnostics. Central to these advancements are synthetic gene circuits, CRISPR-based control systems, RNA regulators, and logic gate architectures enabling high specificity, multiplexed detection, and memory-enabled response. Both whole-cell and cell-free platforms have been developed for detecting pathogens, cancer biomarkers, metabolic imbalances, and environmental contaminants. Notably, wearable and paper-based devices now offer real-time monitoring with minimal infrastructure. In medical applications, engineered biosensors show promise for early diagnosis, personalized treatment monitoring, and integrated theranostics, including systems that trigger therapeutic responses upon biomarker detection. Environmental applications include the detection of heavy metals like arsenic and cadmium via genetically modified microbial platforms. Despite significant advances, challenges remain in circuit stability, biosafety, and large-scale deployment. Future directions involve integrating artificial intelligence, feedback-regulated systems, and hybrid materials to enhance sensor adaptability and performance. This review provides a comprehensive framework for developing next-generation biosensors that merge biological intelligence with engineering precision to meet the growing demand for smart, responsive, and scalable diagnostic tools.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118036"},"PeriodicalIF":10.5,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cationic LIposome capsulated CRISPR-Cas13a Kit (CLICK) for in situ detection of circulating PD-L1 mRNA in single EV towards monitoring of immunotherapy efficacy 阳离子脂质体封装CRISPR-Cas13a Kit (CLICK)原位检测单个EV循环PD-L1 mRNA以监测免疫治疗效果。
IF 10.5 1区 生物学
Biosensors and Bioelectronics Pub Date : 2025-09-26 DOI: 10.1016/j.bios.2025.118032
Yingjing Fan , Weilun Pan , Qi Xiu , Yue Qiao , Peng Zhang , Tong Liao , Junjie Feng , Chengtao Nie , Shuai Chu , Ningcen Li , Xiuhua Wu , Lei Zheng , Zhen Cai , Bo Li
{"title":"Cationic LIposome capsulated CRISPR-Cas13a Kit (CLICK) for in situ detection of circulating PD-L1 mRNA in single EV towards monitoring of immunotherapy efficacy","authors":"Yingjing Fan ,&nbsp;Weilun Pan ,&nbsp;Qi Xiu ,&nbsp;Yue Qiao ,&nbsp;Peng Zhang ,&nbsp;Tong Liao ,&nbsp;Junjie Feng ,&nbsp;Chengtao Nie ,&nbsp;Shuai Chu ,&nbsp;Ningcen Li ,&nbsp;Xiuhua Wu ,&nbsp;Lei Zheng ,&nbsp;Zhen Cai ,&nbsp;Bo Li","doi":"10.1016/j.bios.2025.118032","DOIUrl":"10.1016/j.bios.2025.118032","url":null,"abstract":"<div><div>Tumor immunotherapy has significantly improved cancer treatment, yet immune checkpoint inhibitors only benefit a subset of patients. A critical challenge is non-invasive detection of tumor PD-L1 mRNA, a key biomarker that dynamically reflects PD-L1 expression and correlates with therapeutic outcomes. Current methods are hindered by the instability of circulating mRNA and the need for invasive tissue biopsies. Herein, we introduce the Cationic LIposome capsulated CRISPR-Cas13a Kit (CLICK) for in-situ detection of circulating PD-L1 mRNA in extracellular vesicles (EVs). CLICK achieves direct target identification in 10 μL plasma within 2 h without EV purification or RNA extraction, demonstrating a detection limit of 10<sup>3</sup> EV/mL for PD-L1 mRNA. By integrating membrane fusion-mediated CRISPR delivery with nanoflow cytometry, CLICK resolves PD-L1 mRNA (+) EV subpopulations at single-vesicle resolution, revealing heterogeneous distributions. Clinically validated in 53 immunotherapy patients, CLICK accurately stratifies progressive disease (PD) from stable disease (SD) patients (AUC = 0.8236; 95 % CI: 0.7054–0.9417; p = 0.0006) based on PD-L1 mRNA (+) EV percentages. This work establishes a rapid, blood-based paradigm for therapy response monitoring, advancing liquid biopsy through precise EV RNA characterization.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118032"},"PeriodicalIF":10.5,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ternary hybrids accelerated photoelectrochemical biosensor for sensitive detection of Alzheimer's disease biomarker 用于阿尔茨海默病生物标志物灵敏检测的三元杂化加速光电化学生物传感器
IF 10.5 1区 生物学
Biosensors and Bioelectronics Pub Date : 2025-09-26 DOI: 10.1016/j.bios.2025.118033
Junpeng Gao , Kang Du , Yuyang Xue , Pan Xu , Siqi Ding , Qiumei Feng , Xiaokai Wang
{"title":"Ternary hybrids accelerated photoelectrochemical biosensor for sensitive detection of Alzheimer's disease biomarker","authors":"Junpeng Gao ,&nbsp;Kang Du ,&nbsp;Yuyang Xue ,&nbsp;Pan Xu ,&nbsp;Siqi Ding ,&nbsp;Qiumei Feng ,&nbsp;Xiaokai Wang","doi":"10.1016/j.bios.2025.118033","DOIUrl":"10.1016/j.bios.2025.118033","url":null,"abstract":"<div><div>Amyloid <em>β</em> oligomer (A<em>β</em>O) is not only a crucial biomarker but also a potential therapeutic target for Alzheimer's disease (AD). Here, a type of ternary photoactive hybrids (CdS/ZnS/Bi<sub>2</sub>Se<sub>3</sub>) was used as a substrate material to construct a photoelectrochemical (PEC) biosensor for dual-quenching detection of A<em>β</em>O. Due to the cooperative band gap excitations and multi-interfacial charge transfer of the three components, CdS/ZnS/Bi<sub>2</sub>Se<sub>3</sub> ternary hybrids possessed self-enhanced photocurrent, which significantly boosted PEC responses. The broad-spectrum absorption and peroxidase-mimicking catalytic property of PtCu nanoflowers (NFs) achieved the synergistic dual-quenching effect, which displayed a more sensitive response than common single-quenching PEC biosensors. Impressively, an efficient dynamic signal amplification strategy was established by DNA cascade reaction-activated DNA walker, improving the walk speed and high reaction efficiency. As a result, the constructed biosensor realized the detection of A<em>β</em>O ranging from 20 fM to 10 nM with a detection limit of 4.4 fM. In comparison to previously reported methods, the biosensor enabled the reliable detection of A<em>β</em>O in blood specimens from AD patients and healthy volunteers, demonstrating high sensitivity, specificity, stability, and reproducibility. By simply replacing the aptamer sequences, this strategy could be applied for other biomarker detection with a high potential to inspire innovative sensing approaches, offering some useful help for biomedical research and disease diagnosis.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118033"},"PeriodicalIF":10.5,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dual electrochemical DNA sensor utilizing Ag(I)-Stabilized DNA mismatches for simultaneous, label-free detection of mpox and HIV 双电化学DNA传感器利用Ag(I)稳定的DNA错配,同时,无标记检测mpox和HIV。
IF 10.5 1区 生物学
Biosensors and Bioelectronics Pub Date : 2025-09-26 DOI: 10.1016/j.bios.2025.118035
Piyathida Phimdee , Sudkate Chaiyo , Abdulhadee Yakoh
{"title":"Dual electrochemical DNA sensor utilizing Ag(I)-Stabilized DNA mismatches for simultaneous, label-free detection of mpox and HIV","authors":"Piyathida Phimdee ,&nbsp;Sudkate Chaiyo ,&nbsp;Abdulhadee Yakoh","doi":"10.1016/j.bios.2025.118035","DOIUrl":"10.1016/j.bios.2025.118035","url":null,"abstract":"<div><div>The WHO declared Mpox a public health emergency of international concern due to the resurgence of Mpox clade I outbreaks. Following, the CDC reported a high prevalence of HIV co-infection during the global MPOX outbreak. In 2025, no commercially available multiplex PCR assays enable concurrent detection of mpox and HIV. To address these limitations, this study presents a novel dual electrochemical DNA (eDNA) biosensor for the simultaneous, label-free detection of Mpox and HIV nucleic acids using a mismatched-driven nucleic acid–metal ion nanocomplex (MDN) strategy. The sensor exploits C–C mismatches that selectively coordinate with Ag<sup>+</sup> ions, enabling covalent target capture and sensitive signal generation. It demonstrates a dynamic range of 10<sup>1</sup>–10<sup>5</sup> pM with picomolar-level LODs and successful detection in clinical saliva and urine samples, with LOD below 4 copies/mL. This multiplex platform offers a rapid, cost-effective, and robust diagnostic tool for co-infection screening in high-risk populations.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118035"},"PeriodicalIF":10.5,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145190533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Aggregation-induced electrochemiluminescence aptasensor for ultrasensitive detection of acetamiprid based on polymer dots as emitters 基于聚合物点的聚类诱导电化学发光对啶虫脒超灵敏检测传感器。
IF 10.5 1区 生物学
Biosensors and Bioelectronics Pub Date : 2025-09-25 DOI: 10.1016/j.bios.2025.118031
Yuanyuan Jin, Yerong Zhao, Haibing Zhu, Shijie He, Hang Gao, Lingling Xu, Zhanjun Yang
{"title":"Aggregation-induced electrochemiluminescence aptasensor for ultrasensitive detection of acetamiprid based on polymer dots as emitters","authors":"Yuanyuan Jin,&nbsp;Yerong Zhao,&nbsp;Haibing Zhu,&nbsp;Shijie He,&nbsp;Hang Gao,&nbsp;Lingling Xu,&nbsp;Zhanjun Yang","doi":"10.1016/j.bios.2025.118031","DOIUrl":"10.1016/j.bios.2025.118031","url":null,"abstract":"<div><div>Acetamiprid (ACE) poses significant threats to the environment and human health. Thus, the development of sensitive ACE detection is crucial for safeguarding human health. Herein, we developed an aggregation-induced electrochemiluminescence (AIECL) aptasensor for ACE detection, in which polymer dots (Pdots) are used as luminescent probe. By virtue of the electrochemiluminescence-resonance energy transfer (ECL-RET) mechanism, ECL signal of Pdots is quenched by black hole quencher (BHQ) linked to ACE aptamer, thereby turning “off” the signal of sensor. Upon the existence of ACE, BHQ group releases from the Pdots and escapes from the surface of sensor, consequently enabling the restoration of ECL signal of Pdots. Such sensor exhibits excellent analysis performance with a lower detection limit of 9.1 aM, over conventional ECL analysis for ACE. Significantly, the sensor is applied to lettuce sample with good recovery rate. This work provides an effective method for ACE detection, and validates its application potential in food safety supervision.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118031"},"PeriodicalIF":10.5,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anti-fouling portable electrochemical device for simultaneous detection of CEA and CA15-3 in serum and nipple discharge 同时检测血清和乳头分泌物中CEA和CA15-3的防污便携式电化学装置
IF 10.5 1区 生物学
Biosensors and Bioelectronics Pub Date : 2025-09-25 DOI: 10.1016/j.bios.2025.118030
Shuqi Chen , Cong Wang , Ting Tang , Xiaoru Zhang , Zehua Wang , Xunyi Yuan
{"title":"Anti-fouling portable electrochemical device for simultaneous detection of CEA and CA15-3 in serum and nipple discharge","authors":"Shuqi Chen ,&nbsp;Cong Wang ,&nbsp;Ting Tang ,&nbsp;Xiaoru Zhang ,&nbsp;Zehua Wang ,&nbsp;Xunyi Yuan","doi":"10.1016/j.bios.2025.118030","DOIUrl":"10.1016/j.bios.2025.118030","url":null,"abstract":"<div><div>Early diagnosis of breast cancer is crucial for enhancing patient survival rates. This study developed a portable electrochemical sensor for simultaneous detection of carcinoembryonic antigen (CEA) and cancer antigen 15-3 (CA15-3). Glutaraldehyde cross-linked denatured bovine serum albumin and polydopamine (PDA) was used as an anti-fouling electrochemical sensor interface for screen-printed electrode (SPE). Etched UiO-66-d was employed to adsorb methylene blue and neutral red dyes, followed by modification with phenylboronic acid (PBA) to produce two redox tags. Aptamer DNA and PBA were used to recognize target CEA and CA15-3 to form sandwich structures. To enhance the Point-of-Care Testing (POCT) performance, a portable dual-channel electrochemical detection device was developed integrated with dual-channel SPE for simultaneous detection of CEA and CA15-3. The detection limits for CEA and CA15-3 were determined to be 0.35 pg/mL and 0.0032 U/mL, respectively. From analyzing both serum and nipple discharge samples, it indicates that using nipple discharge as the detection object can effectively differentiate atypical ductal hyperplasia (a pre-breast cancer lesion) from benign hyperplasia, which holds significant implications for early breast cancer diagnosis.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118030"},"PeriodicalIF":10.5,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bioinspired flexible piezoresistive sensor with cross-gradient architecture for high-performance tactile sensing 仿生柔性压阻传感器,具有交叉梯度结构,用于高性能触觉传感。
IF 10.5 1区 生物学
Biosensors and Bioelectronics Pub Date : 2025-09-25 DOI: 10.1016/j.bios.2025.118023
Jiaqi Li , Shihao Chen , Zhenmin Ding , Xu Wang , Ana Sofia Oliveira Henriques Moita , Yan Liu
{"title":"Bioinspired flexible piezoresistive sensor with cross-gradient architecture for high-performance tactile sensing","authors":"Jiaqi Li ,&nbsp;Shihao Chen ,&nbsp;Zhenmin Ding ,&nbsp;Xu Wang ,&nbsp;Ana Sofia Oliveira Henriques Moita ,&nbsp;Yan Liu","doi":"10.1016/j.bios.2025.118023","DOIUrl":"10.1016/j.bios.2025.118023","url":null,"abstract":"<div><div>Tactile perception, as a core technology for flexible sensing, holds significant promise for advanced applications in biomedicine and human-computer interaction. However, achieving the simultaneous optimization of high-performance signal sensing (wide detection range and high sensitivity) and response stability remains a critical challenge for advancing tactile sensors in various applications. Here, inspired by the structural arrangement of snake scales, an innovative flexible tactile sensor with a cross-tilted gradient (CTG) architecture is constructed. The sensor achieves high sensitivity of 2.116 kPa<sup>−1</sup> and a wide detection range of 511.11 kPa through the synergistic effect of an ultra-dense sensing point design and a multi-gradient structural compensation mechanism. Additionally, through interfacial compatibility design and plasma surface treatment, we prepare MXene/PET electrodes with high friction resistance, high conductivity, and strong interface adhesion at room temperature using a flexible electronics direct-write system. The bioinspired sensor exhibits outstanding response characteristics (response time of 8 ms), long-term operational stability (&gt;8500 cycles), and no significant signal drift. Its outstanding sensitivity enables comprehensive human posture sensing. Furthermore, by expanding and integrating individual sensors, high-precision perception of multi-tactile information and effective human-machine interaction are achieved.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118023"},"PeriodicalIF":10.5,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chemiluminescent optical fiber biosensing platform with tyramide signal amplification for ultrasensitive detection of tumor-derived exosomes 具有酰胺信号放大的化学发光光纤生物传感平台用于肿瘤源性外泌体的超灵敏检测。
IF 10.5 1区 生物学
Biosensors and Bioelectronics Pub Date : 2025-09-25 DOI: 10.1016/j.bios.2025.118028
Siqi Zhao , Shuangyu Tang , Bowen Chi , Jinlan Yang , Liande Zhu , Li Yang
{"title":"Chemiluminescent optical fiber biosensing platform with tyramide signal amplification for ultrasensitive detection of tumor-derived exosomes","authors":"Siqi Zhao ,&nbsp;Shuangyu Tang ,&nbsp;Bowen Chi ,&nbsp;Jinlan Yang ,&nbsp;Liande Zhu ,&nbsp;Li Yang","doi":"10.1016/j.bios.2025.118028","DOIUrl":"10.1016/j.bios.2025.118028","url":null,"abstract":"<div><div>Exosomes have emerged as promising non-invasive biomarkers in liquid biopsy for the early diagnosis and treatment of cancers. Traditional methods for exosome analysis are often limited by complex procedures and costly instruments, as well as challenges including low signal differentiation and interference from large amounts of free proteins in body fluids. Herein, we propose a novel biosensing platform that integrates tyramide signal amplification (TSA) with a lipid-anchored chemiluminescent optical fiber sensor (COFS). The platform utilizes distearoyl phosphatylethanolamine-polyethylene glycol (DSPE-PEG) as lipid-anchor to capture exosomes and horseradish peroxidase (HRP)-labeled aptamers for specific recognition. The dual identification of surface proteins and membrane structure avoids interference from free proteins. The TSA-based signal amplification system significantly enhances detection sensitivity, benefiting from the abundance of proteins in exosomal membranes that function as deposition sites for active tyramine. The proposed TSA-COFS platform offers a cost-effective solution to the challenges of exosome quantitation, enabling rapid analysis, ultrasensitive detection and point-of-care profiling of tumor-derived exosomes within biological samples. The efficacy of the platform was demonstrated by analyzing Mucin 1 protein (MUC1)-expressing exosomes from human clinical serum samples. The results show that the method can achieve rapid and accurate determination of exosomes with high selectivity and an extremely low limit-of-detection of 6.76 particles/μL. A home-made portable device, equipped with the sensor probe, a \"plug-in\" operation of the reagent strip, a battery-powered photon counter and a touch-screen computer, is used for the assays, demonstrating the potential practical value of the TSA-COFS for exosome quantitation and point-of-care testing (POCT) applications.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118028"},"PeriodicalIF":10.5,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Electrochemiluminescence-colorimetric dual-mode biosensor based on CeO2:Eu3+ nanozyme amplification for the detection of glucose 基于CeO2:Eu3+纳米酶扩增的电化学发光-比色双模生物传感器检测葡萄糖。
IF 10.5 1区 生物学
Biosensors and Bioelectronics Pub Date : 2025-09-25 DOI: 10.1016/j.bios.2025.118027
Mingxia Wang, Minggang Wei, Weiling Su, Liying Liu, Yanhong Li, Lijun Zhao, Feng Luan, Xuming Zhuang, Chunyuan Tian
{"title":"Electrochemiluminescence-colorimetric dual-mode biosensor based on CeO2:Eu3+ nanozyme amplification for the detection of glucose","authors":"Mingxia Wang,&nbsp;Minggang Wei,&nbsp;Weiling Su,&nbsp;Liying Liu,&nbsp;Yanhong Li,&nbsp;Lijun Zhao,&nbsp;Feng Luan,&nbsp;Xuming Zhuang,&nbsp;Chunyuan Tian","doi":"10.1016/j.bios.2025.118027","DOIUrl":"10.1016/j.bios.2025.118027","url":null,"abstract":"<div><div>Conventional glucose (Glu) sensing platforms predominantly rely on single-signal readouts, a strategy inherently vulnerable to matrix interference and this limitation often leads to inaccurate quantification when analyzing complex biological or environmental samples. To address this challenge, we herein developed an electrochemiluminescence (ECL)-colorimetric dual-mode detection strategy for Glu, based on CeO<sub>2</sub>:Eu<sup>3+</sup> nanomaterials, with the aim of enhancing the accuracy of Glu quantification. The CeO<sub>2</sub>:Eu<sup>3+</sup> nanomaterials exhibited excellent ECL performance in an ECL system using potassium persulfate (K<sub>2</sub>S<sub>2</sub>O<sub>8</sub>) as the coreactant. Notably, their ECL intensity could be effectively quenched by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), and the quenching efficiency showed a linear correlation with H<sub>2</sub>O<sub>2</sub> concentration, this relationship enabled the quantitative detection of H<sub>2</sub>O<sub>2</sub>. Furthermore, the peroxidase-mimetic activity of CeO<sub>2</sub>:Eu<sup>3+</sup> nanomaterials further enhanced the ECL quenching efficiency, thereby improving the sensitivity of H<sub>2</sub>O<sub>2</sub> detection. Concurrently, the CeO<sub>2</sub>:Eu<sup>3+</sup> nanomaterials displayed remarkable peroxidase-mimetic activity in the chromogenic reaction between 3,3′,5,5′-tetramethylbenzidine (TMB) and H<sub>2</sub>O<sub>2</sub>, inducing a distinct color transition from colorless to blue that could be easily visualized and recorded using a smartphone. Leveraging these properties of CeO<sub>2</sub>:Eu<sup>3+</sup> nanomaterials, combined with the reaction mechanism wherein Glu generates H<sub>2</sub>O<sub>2</sub> under the catalysis of Glu oxidase (GOx), we established a dual-mode detection method for Glu. Under optimized experimental conditions, this method achieved highly sensitive and selective Glu detection: the limit of detection (LOD) was as low as 0.033 nM for the ECL mode and 1.46 μM for the colorimetric mode (S/N = 3). This work integrates the unique dual functionalities of CeO<sub>2</sub>:Eu<sup>3+</sup> nanomaterials to realize sensitive dual-mode Glu detection, providing new insights for the innovative design of dual-mode sensors and exhibiting significant potential in clinical diabetes monitoring.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118027"},"PeriodicalIF":10.5,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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