Biosensors and Bioelectronics最新文献

{"title":"One-pot RPA-CRISPR/Cas12a integrated dual-mode electrochemical lateral flow strip for ultrasensitive and precise detection of Salmonella","authors":"Qi Chen ,&nbsp;Hang Wang ,&nbsp;Haoyang Xu ,&nbsp;Yubo Peng ,&nbsp;Bangben Yao ,&nbsp;Zhaoran Chen ,&nbsp;Jiening Yang ,&nbsp;Samuel Adeloju ,&nbsp;Wei Chen","doi":"10.1016/j.bios.2025.117529","DOIUrl":"10.1016/j.bios.2025.117529","url":null,"abstract":"<div><div>Rapid and accurate screening of pathogenic contamination is essential for timely intervention and infection prevention. In this work, one-pot RPA-CRISPR/Cas12a strategy combined with an innovative electrochemical lateral flow strip (OPRCC-eLFS) was presented for ultrasensitive and precise detection of <em>Salmonella</em>. Highly sensitive dual-mode detection of <em>Salmonella</em> in various samples has been simultaneously achieved with electrochemical detection limit of 3.84 CFU/mL and visual detection limit of 384 CFU/mL, respectively, with improved detection efficiency and prevention of uncapping-related aerosol-contamination. This dual-mode biosensing platform demonstrates exceptional stability, remarkable sensitivity, and robust on-site quantification capability, emphasizing its potential in food safety monitoring and disease prevention.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"285 ","pages":"Article 117529"},"PeriodicalIF":10.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144069418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bifunctional S-doping-mediated interfacial gradient electric field for in-situ amplified photoelectrochemical immunoassay","authors":"Bin Li ,&nbsp;Jia Lin ,&nbsp;Cheng Cheng ,&nbsp;Haowen Zhang ,&nbsp;Yuanyuan Guo ,&nbsp;Yingru Zhi ,&nbsp;Fan Cai ,&nbsp;Yitong Zhang ,&nbsp;Zhe Di ,&nbsp;Houxi Xu ,&nbsp;Ziyi Zhou ,&nbsp;Wei Qin ,&nbsp;Dongfeng Wei ,&nbsp;Yaoyao Bian ,&nbsp;Guisheng Zhou ,&nbsp;Jing Chen ,&nbsp;Lilin Ge ,&nbsp;Yao Lin","doi":"10.1016/j.bios.2025.117531","DOIUrl":"10.1016/j.bios.2025.117531","url":null,"abstract":"<div><div>Ultrasensitive chemical reactions at the photoanode interface provide new ideas for the development of novel photoelectrochemical (PEC) immunoassays. Herein, we reported an <em>in situ</em>-promoted all-inorganic semiconductor reaction realizing an ultrasensitive PEC analysis of carcinoembryonic antigen (CEA). Uniform In₂O₃ nanocubes were synthesized through one-step <em>in situ</em> growth, and composite In₂O_xS_3-x was obtained by one-step post-modification sulfurization, achieving ultra-high light-to-dark current switching ratios (169 times). S doping, on the one hand, lowered the band gap of In₂O₃ and established a gradient electric field to enhance charge separation, resulting in a substantial enhancement of the photocurrent; on the other hand, it reacted with Cu²⁺ released from the detection probes during the detection process to further amplify the photocurrent signal. The presence of a built-in gradient electric field of In₂O_xS_3-x was determined by <em>in situ</em> X-ray photoelectron spectroscopy (XPS) and density functional theory (DFT). In the presence of CEA, CuO modified on the detection probe formed Cu²⁺ by exogenous acidification and therefore caused a sudden crossing of the photocurrent by forming a robust Cu-S bond with the vulcanized photoanode. Under optimized conditions, the developed PEC immunosensing system based on photoanodic interfacial reaction exhibited an ultra-wide operating range (0.05–100 ng mL⁻¹), and an ultra-low limit of detection (13.5 pg mL⁻¹). In conclusion, this work provides a promising <em>in situ</em> ultrasensitive monitoring strategy for efficient PEC bio-immunosensor, expanding the range of potential applications in early cancer analysis and bedside diagnostics.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"283 ","pages":"Article 117531"},"PeriodicalIF":10.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Enzyme-accelerated catalytic DNA circuits enable rapid and one-pot detection of bacterial pathogens” [Biosens. Bioelectron. 267 (2025) 116822]","authors":"Baolin Li ,&nbsp;Hui Jiang ,&nbsp;Sijian Luo ,&nbsp;Zhangrui Zeng ,&nbsp;Xuejing Xu ,&nbsp;Xinyu Li ,&nbsp;Songzhi Zhang ,&nbsp;Yirong Chen ,&nbsp;Shijia Ding ,&nbsp;Xinmin Li ,&nbsp;Jinbo Liu ,&nbsp;Wei Chen","doi":"10.1016/j.bios.2025.117511","DOIUrl":"10.1016/j.bios.2025.117511","url":null,"abstract":"","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"282 ","pages":"Article 117511"},"PeriodicalIF":10.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micropillar array-based microfluidic device for electrochemical monitoring of cell culture health","authors":"Masoud Khazaei ,&nbsp;Jann Harberts ,&nbsp;Azadeh Nilghaz ,&nbsp;Michael Shola David ,&nbsp;Kenneth Galbraith ,&nbsp;Muamer Dervisevic ,&nbsp;Victor J. Cadarso ,&nbsp;Nicolas H. Voelcker","doi":"10.1016/j.bios.2025.117534","DOIUrl":"10.1016/j.bios.2025.117534","url":null,"abstract":"<div><div>Glucose levels serve as a fundamental indicator of cell health, reflecting crucial aspects of cellular metabolism and energy production. While effective, traditional methods such as spectrophotometry and chromatography have limitations, such as labour-intensive sample collection, reliance on bulky equipment, extensive sample preparation, and prolonged experimental durations. To address these issues, we introduce a micropillar-based microfluidic electrochemical device (MED) for real-time monitoring of glucose levels in diverse cell culture systems, including human induced pluripotent stem cells (hiPSCs) and murine fibroblast cells (GP + E86). This biosensor demonstrates a linear range of 0.025–1.50 mM and a high sensitivity of 4.71 ± 0.13 μA. mM⁻¹, and a low limit of detection of 19.10 ± 0.50 μM. The MED not only delivered fast glucose measurements with accuracy and reliability comparable to ultra-high-performance liquid chromatography (UHPLC) but was also specifically evaluated on GP + E86 murine fibroblast cells at varying seeding densities (1:5 and 1:10 ratios), across different culturing times to accurately monitor dynamic metabolic shifts associated with various growth phases. Furthermore, the MED effectively detected significant changes in glucose consumption in hiPSCs cell cultures contaminated with <em>Escherichia coli (E. coli)</em>, highlighting its potential for early contamination detection. Integrating non-invasive, continuous monitoring platforms enhances the reliability of experimental outcomes by enabling cell health monitoring without disrupting the cell culture process. This approach enables real-time monitoring of cell cultures ensuring accurate detection of metabolic changes and early detection of media contamination.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"283 ","pages":"Article 117534"},"PeriodicalIF":10.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photothermal effect-assisted reduced graphene oxide biosensor for amplification-free detection of miRNA","authors":"Wenrui Xu,&nbsp;Xiaoguang Gao,&nbsp;Yuyan Zhai,&nbsp;Xiaoliang Zhang,&nbsp;Yingqian Wang,&nbsp;Rong Deng,&nbsp;Xiaochun Li","doi":"10.1016/j.bios.2025.117532","DOIUrl":"10.1016/j.bios.2025.117532","url":null,"abstract":"<div><div>It remains a challenge to achieve high-sensitivity detection of tumor marker miRNA using optical refractive index (RI) sensors without nucleic acid amplification. This study proposes the photothermal effect-assisted reduced graphene oxide (rGO) biosensor that combines the photothermal effect of rGO with the rGO-based RI sensor for high-sensitivity detection of tumor marker miRNA-21. The rGO was functionalized with DNA probes capable of specifically hybridizing with the target miRNA-21. Quantitative detection of miRNA-21 was achieved by monitoring the RI change caused by the competitive hybridization of single-strand DNA (ssDNA)-functionalized AuNPs and target miRNA-21 with the DNA probes on the rGO surface. The presence of AuNPs disturbed the evanescent field on the rGO surface, thus achieving signal amplification. Furthermore, the localized photothermal effect heat induced by the interaction between rGO and pump light can effectively improve the hybridization kinetics of nucleic acid chains and achieve further signal amplification. The proposed biosensor had a high sensitivity toward the target miRNA-21, achieving a low detection limit of 4.05 fM without nucleic acid amplification. Its high specificity allowed for the recognition of single-base mismatches in miRNA-21. In addition, accurate quantification of low abundance miRNA-21 spiked into human urine samples was also successfully achieved. The photothermal effect-assisted rGO biosensor offers a promising approach for high-sensitivity detection of tumor marker miRNA without need for nucleic acid amplification.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"284 ","pages":"Article 117532"},"PeriodicalIF":10.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143924687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Tunable aptamer-MXene sensing interface for label-free and real-time detection of toxic pollutants in water samples” [Biosens. Bioelectron. 271 (2025) 117096]","authors":"Jinghua Liu ,&nbsp;Feng Tan ,&nbsp;Tianhao Cao ,&nbsp;Runqiang Yu ,&nbsp;Yan Wang","doi":"10.1016/j.bios.2025.117523","DOIUrl":"10.1016/j.bios.2025.117523","url":null,"abstract":"","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"282 ","pages":"Article 117523"},"PeriodicalIF":10.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction of cardiac differentiation in human induced pluripotent stem cell-derived cardiomyocyte supernatant using surface-enhanced Raman spectroscopy and machine learning","authors":"Karla Echeverría-Altamar ,&nbsp;Carlos Barreto-Gamarra ,&nbsp;Maribella Domenech-García ,&nbsp;Pedro Resto-Irizarry","doi":"10.1016/j.bios.2025.117528","DOIUrl":"10.1016/j.bios.2025.117528","url":null,"abstract":"<div><div>The efficient manufacturing of cardiomyocytes from human-induced pluripotent stem cells (hiPSCs) is essential for advancing regenerative therapies for myocardial injuries. However, ensuring cell quality during production is challenging since traditional methods are invasive, destructive, and time-consuming. In this study, we monitored cardiomyocyte differentiation of WTC11 hiPSCs by analyzing conditioned media collected at various stages using Raman spectroscopy, multivariate analysis, and machine learning. Differentiation efficiency was confirmed via flow cytometry and immunostaining. Raman spectra were processed using standard normal variate and second derivative transformations before performing a principal component analysis (PCA) and machine learning (Random Forest, K-Nearest Neighbors, and Deep Neural Networks [DNN]). Results show that PCA was unable to distinguish cells based on differentiation stages, while machine learning could reliably predict cell differentiation early in the cardiac cell manufacturing process. DNN models achieved accuracies exceeding 82 % in predicting differentiation, highlighting their potential as quality control tools. These findings underscore the potential of Raman spectroscopy coupled with machine learning as a tool for real-time monitoring of cardiomyocyte production.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"283 ","pages":"Article 117528"},"PeriodicalIF":10.7,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143911646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smart bandages for wound monitoring and treatment","authors":"Ping Wu ,&nbsp;Long Jin ,&nbsp;Wanying Jiang ,&nbsp;Yingzhang Zhou ,&nbsp;Lisheng Lin ,&nbsp;Hongxin Lin ,&nbsp;Hu Chen","doi":"10.1016/j.bios.2025.117522","DOIUrl":"10.1016/j.bios.2025.117522","url":null,"abstract":"<div><div>Wound management plays a crucial role in nursing care as it facilitates effective wound healing and prevents infections. To overcome limitations associated with traditional treatment methods, various smart bandages have been developed. The monitoring of wound parameters and the implementation of targeted treatments are crucial aspects of smart bandage development. Smart bandages, as cutting-edge flexible wearable medical devices, integrate various sensing technologies, providing new insights for dynamic monitoring and personalized treatment of chronic wounds. This paper systematically summarizes the applications and developments of smart bandages in monitoring wound environmental parameters, focusing on two major detection methods: colorimetric sensing and electrochemical sensing. Colorimetric sensors typically rely on color changes induced by physiological parameters, which can not only be identified by the naked eye but also combined with image recognition algorithms for physiological parameter detection. Electrochemical sensors, on the other hand, modify electrodes with specific enzymes and detect physiological parameters through the electrical signals generated by redox reactions. In addition to sensing, this paper further explores the integrated application of three smart therapeutic strategies in smart bandages, including promoting cell proliferation and angiogenesis through electrical stimulation, achieving controlled drug release <em>via</em> responsive materials, and utilizing photothermal materials for efficient antibacterial treatment of wounds. Finally, the paper delves into the challenges these bandages face in system integration and clinical translation, and discusses their potential in personalized wound care.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"283 ","pages":"Article 117522"},"PeriodicalIF":10.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143907946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyaniline-based hybrid membrane for single-molecule protein nanopore analysis under high voltage","authors":"Xingtong Liu,&nbsp;Xinyue Li,&nbsp;Zhenxu Zhu,&nbsp;Fujun Yao,&nbsp;Xiao-feng Kang","doi":"10.1016/j.bios.2025.117520","DOIUrl":"10.1016/j.bios.2025.117520","url":null,"abstract":"<div><div>In vitro reconstituted lipid bilayers are the key to the birth of bio-nanopore technology, although they are fragile and inadequately stable for long-term monitoring applications. Amphiphilic copolymers with high robustness have been developed to circumvent this issue. However, it remains a major challenge to achieve the required fluidity for protein insertion. Here we engineered a polyaniline-based hybrid membrane (PANIM) that exhibits the stability needed for fabricating high-throughput biosensors. This enhanced durability enables prolonged operation without compromising the functional properties of embedded <em>α</em>-hemolysin (<em>α</em>-HL) nanopores. Based on this, we demonstrate high voltage analysis for G-triplex conformations through <em>α</em>-HL in PANIM, which facilitates in-depth study of molecular conformations and provides a novel platform that has great potential for nanopore sensing.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"283 ","pages":"Article 117520"},"PeriodicalIF":10.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A CRISPR/Cas12a mediated click immunoassay catalyzed by in situ formation of clickase for highly sensitive detection of Trichinella spiralis","authors":"Yao Yu ,&nbsp;Yi Liu ,&nbsp;Ning Xu ,&nbsp;Lin Li ,&nbsp;Yaming Yang ,&nbsp;Xiaolei Liu ,&nbsp;Lianjing Zhao ,&nbsp;Xue Bai","doi":"10.1016/j.bios.2025.117521","DOIUrl":"10.1016/j.bios.2025.117521","url":null,"abstract":"<div><div>The accurate and high-throughput detection of trace protein targets remains an ongoing challenge in the field of food safety testing. This research article presents a highly sensitive CRISPR/Cas12a-mediated click immunoassay for the sensitive detection of <em>Trichinella spiralis</em> (<em>T. spiralis</em>) in meat samples. By simultaneously conjugating activator ssDNA and monoclonal antibodies to gold nanoparticles, the CRISPR/Cas12a system was introduced into the immunoassay. To overcome the challenges associated with the preparation, storage, and transportation of FQ probes in CRISPR/Cas12a systems, the Copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reaction was employed instead. The designed ssDNA was both the substrate of trans-cleavage activity of Cas12a and the synthetic template of the artificial clicking enzyme copper nanoparticles (CuNPs), which could effectively catalyze the CuAAC reaction to generate the desired signal output. The fluorescence intensity showed a linear relationship with <em>T. spiralis</em> crude protein concentration ranging from 3.125 to 100 ng/mL, and the detection limit was 0.35 ng/mL, which is three orders of magnitude lower than that of ELISA (LOD: 309.75 ng/mL). This method can accurately detect a single <em>T. spiralis</em> larva in 100 g of pork. Collectively, the strategy of combining CRISPR/Cas12a system and CuAAC reaction opens up a novel avenue for developing a highly sensitive, simple and convenient fluorescence assay.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"283 ","pages":"Article 117521"},"PeriodicalIF":10.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143888204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}