Biosensors and Bioelectronics最新文献_第3页

Triple-signal lateral flow immunoassay for the detection of bovine casein using an M13 phage-displayed nanobody and MnO2 nanozyme conjugate 利用M13噬菌体纳米体和MnO2纳米酶偶联物的三信号侧流免疫分析法检测牛酪蛋白。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-06-01 Epub Date: 2026-02-09 DOI: 10.1016/j.bios.2026.118486

Wenyi Dong , Ajuan You , Yulou Qiu , Wanwan Liu , Jia Qiu , Yutao Zhu , Biao Zhang , Xianshu Fu , Zihong Ye , Xiaoping Yu

{"title":"Triple-signal lateral flow immunoassay for the detection of bovine casein using an M13 phage-displayed nanobody and MnO2 nanozyme conjugate","authors":"Wenyi Dong , Ajuan You , Yulou Qiu , Wanwan Liu , Jia Qiu , Yutao Zhu , Biao Zhang , Xianshu Fu , Zihong Ye , Xiaoping Yu","doi":"10.1016/j.bios.2026.118486","DOIUrl":"10.1016/j.bios.2026.118486","url":null,"abstract":"<div><div>Cow's milk allergy is a significant global health concern, with bovine casein recognized as its predominant allergen. Rapid, sensitive, and accurate detection of bovine casein in food products is therefore essential. Here, a triple-signal lateral flow immunoassay (LFIA) for casein detection was developed by integrating an M13 phage-displayed nanobody (M13-Nb) with multifunctional MnO<sub>2</sub> nanosheets (MnO<sub>2</sub> NSs) as the signal probe. Anti-casein M13-Nb was selected from a naïve phage-displayed nanobody library through four rounds of biopanning. MnO<sub>2</sub> NSs exhibiting intrinsic colorimetric properties, oxidase-like catalytic activity, and photothermal capability were synthesized and conjugated with M13-Nb, enabling the MnO<sub>2</sub> NSs@M13-Nb-based LFIA to generate colorimetric, amplified colorimetric, and photothermal signals simultaneously. The visual limits of detection (vLOD) for these modes were 125, 15.63, and 31.25 ng/mL, respectively, with corresponding quantitative LODs of 60.97, 6.72, and 23.88 ng/mL. The assay showed high specificity and accuracy, with recovery rates of 87.92%–114.25% in spiked food samples. This triple-signal LFIA provides a rapid and reliable platform for casein detection in foods and highlights the potential of combining M13-Nb and MnO<sub>2</sub> NSs for on-site monitoring of food allergens.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118486"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146177172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Affordable plasmonic biosensing: democratizing SERS with scalable, field-compatible substrate fabrication 经济实惠的等离子体生物传感：民主化SERS与可扩展，场兼容的衬底制造

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-06-01 Epub Date: 2026-02-05 DOI: 10.1016/j.bios.2026.118487

Saransh Arora , Vighnesh Ginde , Swati Tanwar , Marwan El Chazli , Raj Bhatt , Evan Edelman , Debadrita Paria , Ishan Barman

{"title":"Affordable plasmonic biosensing: democratizing SERS with scalable, field-compatible substrate fabrication","authors":"Saransh Arora , Vighnesh Ginde , Swati Tanwar , Marwan El Chazli , Raj Bhatt , Evan Edelman , Debadrita Paria , Ishan Barman","doi":"10.1016/j.bios.2026.118487","DOIUrl":"10.1016/j.bios.2026.118487","url":null,"abstract":"<div><div>Efficient and accurate plasmonic biosensing in the field remains a challenge. Despite its potential to revolutionize point-of-care (PoC) diagnostics through unparalleled sensitivity and precise molecular fingerprinting, adoption of traditional surface-enhanced Raman spectroscopy (SERS) has not proven feasible in the field. High production costs, complex fabrication, specialized equipment, and persistent issues with stability and reproducibility impede development for PoC use. Addressing these challenges, this study innovates a democratized and cost-effective fabrication kit that enables the SERS substrate production using commonly available materials and straightforward electrochemistry without compromising on sensitivity and reproducibility. Importantly, this method leverages commercially available bottled water and simple battery-powered fabrication, eliminating reliance on a power grid and enabling local production of biosensors in resource-restricted regions. The cost of producing the fabrication kit is $39.54 with raw materials purchased at bulk retail prices, while the cost of consumables for fabricating each test is just 1.33¢. We demonstrate the efficacy of these substrates in two critical applications: the rapid detection of bacteria and pesticides. We detect trace levels of pesticides such as Thiram and Thiabendazole down to 0.1 ppm using a digital SERS approach. We also demonstrated the identification of bacteria isolated from culture, namely <em>Escherichia coli</em> and <em>Bacillus subtilis.</em> We envision that this label-free, high-sensitivity substrate, when paired with portable Raman spectrometers, could open doors for a new era of field-deployable biosensing, paving the way for its adoption for a plethora of applications, from public health to food testing.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118487"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Classification of liver tissue pathological changes via optical biopsy based on refractive index sensing 基于折射率传感的肝组织病理活检分类。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-06-01 Epub Date: 2026-02-09 DOI: 10.1016/j.bios.2026.118508

Kacper Cierpiak , Sebastián García-Galán , Jakub Czubek , Rafal Urniaz , Małgorzata Szczerska

{"title":"Classification of liver tissue pathological changes via optical biopsy based on refractive index sensing","authors":"Kacper Cierpiak , Sebastián García-Galán , Jakub Czubek , Rafal Urniaz , Małgorzata Szczerska","doi":"10.1016/j.bios.2026.118508","DOIUrl":"10.1016/j.bios.2026.118508","url":null,"abstract":"<div><div>Optical biopsy enables minimally invasive, quantitative tissue assessment, yet clinically useful implementations require rapid and objective decision-making from compact sensors. We present a refractive-index (RI) driven classification framework based on reflection spectra acquired with an extrinsic fiber-optic Fabry–Pérot interferometric cavity (280 μm) over the biologically relevant RI range 1.33–1.42. Three proxy classes (“healthy”, “HCC-like”, “metastatic”) were defined using literature-guided RI windows for liver tissue, and measurements were performed on certified reference liquids. As a physics-only reference, RI was estimated analytically from fringe periodicity in the wavenumber domain, achieving 0.70 accuracy and 0.48 macro-F1. To enhance discrimination, we engineered 62 spectral descriptors capturing fringe spacing (RI-related), fringe visibility, and spectral-shape cues, and trained tree-ensemble and SVM models together with an interpretable GA-optimized fuzzy expert system. On a held-out test set, tree ensembles reached macro-F1 = 1.00, while SVM and the fuzzy system achieved 0.96 and 0.97, respectively. Feature attribution identified RI as the dominant discriminative signal, with visibility-related metrics improving robustness near the HCC-like boundary. These results demonstrate that ML-augmented fiber-optic interferometry can deliver accurate and explainable diagnostic signatures, supporting the translational potential of RI-based optical biopsy.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118508"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146177040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in wearable biophysical sensors for continuous muscle monitoring 用于连续肌肉监测的可穿戴生物物理传感器的最新进展。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-06-01 Epub Date: 2026-02-04 DOI: 10.1016/j.bios.2026.118463

Runjia Zheng , Haihua Xu

{"title":"Recent advances in wearable biophysical sensors for continuous muscle monitoring","authors":"Runjia Zheng , Haihua Xu","doi":"10.1016/j.bios.2026.118463","DOIUrl":"10.1016/j.bios.2026.118463","url":null,"abstract":"<div><div>Continuous muscle monitoring is paramount for revealing the dynamics of neuromuscular behaviors including fatigue kinetics, muscle recruitment, coordination strategies, and movement adaptations. This capability is fundamental for advancing neurorehabilitation, injury prevention and human-machine interfaces. Importantly, the drive towards wearable muscle sensors is rapidly expanding the feasibility and scope of continuous monitoring applications. This paper highlights recent advancements in wearable muscle sensors spanning electrical, biomechanical, acoustic, and optical sensing domains. Innovations in soft materials and flexible electronics have enabled the development of skin-conformal sensors achieving high-fidelity signal capture. We demonstrate significant advances including enhanced surface electromyography (sEMG) electrodes, ultra-sensitive strain/pressure sensors for muscle deformation monitoring, miniaturized wearable ultrasound transducers for deep-tissue imaging, and near-infrared spectroscopy (NIRS) devices for muscle oxygenation monitoring. We also examine representative multimodal integrations of these modalities, providing the possibility for unprecedented, comprehensive assessment of muscle function. The challenges and prospects are further put forward, including stable skin–sensor interfaces, energy autonomy for continuous operation, accurate decoding of complex multimodal signals, seamless integration with artificial intelligence (AI)-driven analytics and active intervention. This study provides a comprehensive framework for continuous muscle monitoring techniques, and thus paves the way for advancing clinical diagnostics, rehabilitation, and human-machine interaction.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118463"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146177111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intramolecular entropy-driven amplifier assembled on a DNA octahedron enables rapid imaging of dual cancer-related long noncoding RNAs 组装在DNA八面体上的分子内熵驱动放大器能够快速成像双重癌症相关的长链非编码rna

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-06-01 Epub Date: 2026-02-06 DOI: 10.1016/j.bios.2026.118477

Xiao-fei Liu , Huimin Yuan , Jun Lu , Fei Ma , Chun-yang Zhang

{"title":"Intramolecular entropy-driven amplifier assembled on a DNA octahedron enables rapid imaging of dual cancer-related long noncoding RNAs","authors":"Xiao-fei Liu , Huimin Yuan , Jun Lu , Fei Ma , Chun-yang Zhang","doi":"10.1016/j.bios.2026.118477","DOIUrl":"10.1016/j.bios.2026.118477","url":null,"abstract":"<div><div>Long noncoding RNAs (lncRNAs) are a category of pivotal transcriptional regulators, and their dysregulation is correlated with various cancers. The reaction rate of traditional entropy-driven catalysis (EDC) relies on the diffusion of free reactants, resulting in slow reaction kinetics. Herein, we engineer an intramolecular entropy-driven amplifier assembled on a DNA octahedron for rapid imaging of dual cancer-related lncRNAs. In this amplifier, the DNA octahedron strategically incorporates six different functional modules on each of its six vertices. The six functional modules can be divided into three groups, including two binding modules for tumor cell capture, two recognition modules for lncRNA identification, and two amplification modules for fuel-strand-driven signal amplification. The engineering of the DNA octahedron not only integrates all functional modules into a single molecular scaffold, but also provides abundant sites for the simultaneous detection of dual cancer-related lncRNAs. This integration can effectively accelerate the reaction kinetics due to the spatial confinement effect of the DNA octahedron. The assay can be completed within 80 min, and achieves limits of detection (LOD) of 0.66 fM for the MALAT1 assay and 0.93 fM for the HOTAIR assay. Moreover, it enables not only single-cell quantification and accurate discrimination of MALAT1 and HOTAIR in cancerous and normal breast tissues, but also rapid imaging of dual lncRNAs in living cells. This proof-of-concept work shows promising potential in clinical diagnostics and postoperative monitoring.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118477"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146147295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering a self-sufficient ECL system: Oxygen-vacancy-driven O2 generation and nanozyme confinement for bioanalysis 设计一个自给自足的ECL系统：氧空缺驱动的O2生成和纳米酶限制用于生物分析。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-06-01 Epub Date: 2026-02-07 DOI: 10.1016/j.bios.2026.118491

Nastaran Arab , Morteza Hosseini , Guobao Xu , Hodjattallah Rabbani

{"title":"Engineering a self-sufficient ECL system: Oxygen-vacancy-driven O2 generation and nanozyme confinement for bioanalysis","authors":"Nastaran Arab , Morteza Hosseini , Guobao Xu , Hodjattallah Rabbani","doi":"10.1016/j.bios.2026.118491","DOIUrl":"10.1016/j.bios.2026.118491","url":null,"abstract":"<div><div>Oxygen vacancies in nonnoble metal nanocatalysts, while extensively explored for oxygen evolution reactions (OER), have rarely been harnessed for electrochemiluminescence (ECL) immunosensing. In this work, we report the rational construction of a novel Fe–Co–CeO<sub>2</sub>@Ti<sub>3</sub>C<sub>2</sub>(OH)<sub>2</sub> heterostructure, incorporating Fe–Co dual active sites and abundant oxygen vacancies, through an energy-free co-deposition strategy for efficient OER under near-neutral conditions. Notably, Fe–Co–CeO<sub>2</sub>@Ti<sub>3</sub>C<sub>2</sub>(OH)<sub>2</sub> heterostructure not only enhances OER activity but also the oxygen vacancy enriched CeO<sub>2</sub> nanoparticles of the heterostructure facilitate superoxide radical (O<sub>2</sub>•<sup>-</sup>) generation within the luminol system in the absence of conventional co-reactants (e.g., H<sub>2</sub>O<sub>2</sub> or dissolved O<sub>2</sub>). In addition, FeCo-NH<sub>2</sub>-BDC was employed as a signaling probe, whose intrinsic oxidase mimetic activity catalyzes O<sub>2</sub> to O<sub>2</sub>•<sup>-</sup>, further amplifying the ECL response. By integrating these multiple amplification pathways, we developed a novel biosensing platform for the highly sensitive detection of carcinoembryonic antigen (CEA). The ECL signals were quantified using both a photomultiplier tube (PMT) and a smartphone based visual readout, achieving linear response ranges of 10 pg mL<sup>−1</sup>–25 ng mL<sup>−1</sup> (LOD: 6.35 pg mL<sup>−1</sup>) and 35 pg mL<sup>−1</sup>–50 ng mL<sup>−1</sup> (LOD: 12.42 pg mL<sup>−1</sup>), respectively. This work provides a powerful approach for high performance bioanalysis and offers a promising avenue toward the development of ultrasensitive, user friendly diagnostic devices.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118491"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146177102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CuxS@SRB bionanohybrid for ultrasensitive electrochemical detection of coenzyme F420: a specific biomarker for Methanogenic archaea CuxS@SRB用于超灵敏电化学检测辅酶F420的bionanohybrid：一种产甲烷古菌的特异性生物标志物

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-06-01 Epub Date: 2026-02-08 DOI: 10.1016/j.bios.2026.118505

Xueqi Hu , You Xu , Xiaoling Liu , Mengna Dong , Airong Zhang , Jiawei Dai , Zhuo Huang , Davide Raffaele Ceratti , Hongfang Liu , Guangfang Li

{"title":"CuxS@SRB bionanohybrid for ultrasensitive electrochemical detection of coenzyme F420: a specific biomarker for Methanogenic archaea","authors":"Xueqi Hu , You Xu , Xiaoling Liu , Mengna Dong , Airong Zhang , Jiawei Dai , Zhuo Huang , Davide Raffaele Ceratti , Hongfang Liu , Guangfang Li","doi":"10.1016/j.bios.2026.118505","DOIUrl":"10.1016/j.bios.2026.118505","url":null,"abstract":"<div><div>Methanogenic archaea play a vital role in global methane emission as well as corrosion of oil and gas field equipment. A real-time detection of methanogenic archaea is pivotal for predicting methane (CH<sub>4</sub>) emission and providing an early warning of microbiological corrosion. While traditional detection methods often take extensive processing time and yield inaccurate results, here, we developed a rapid electrochemical bionanohybrid sensor of the specific coenzyme F<sub>420</sub> of the methanogenic archea, by <em>in-situ</em> growth of copper sulfide (Cu<sub>x</sub>S) nanoparticles on electroactive sulfate-reducing bacteria (SRB) in incubation culture. A negatively linear relationship between electroreduction current density and F<sub>420</sub> concentration over Cu<sub>x</sub>S@SRB bionanohybrid was found in an ultrawide range of 0.01-1000 nM with an ultralow detection limit of 2.8 pM (S/N = 3). The bionanohybrid exhibited strong robustness against interference by pH variations, temperature changes and other chemicals. We explain the excellent electrochemical sensing through a specific chemisorption due to a Lewis acid-base interaction between Cu<sup>+</sup> of Cu<sub>x</sub>S and nitrogen centers in coenzyme F<sub>420</sub>. The nanohybrid sensor enabled direct quantification of <em>M. maripaludis</em> between 10<sup>0</sup>-10<sup>7</sup> CFU/mL with a detection limit of 1.8 CFU/mL with a 96.65-103.90% recovery in real pond. This study establishes a new paradigm for rapid detection of methanogenic archaea by exploiting electroactive microorganisms which construct high-performance bionanohybrid electrochemical sensor. This approach offers a broad application in methane emission, carbon cycle analysis, and microbial corrosion early warning systems.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118505"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-cycle signal amplification on an Au@CeO2-x/Bi2MoO6 platform: Toward an ultrasensitive and enzyme-free sandwich immunosensor for CA125 in clinical diagnostics Au@CeO2-x/Bi2MoO6平台上的双周期信号放大：用于CA125临床诊断的超灵敏无酶三明治免疫传感器

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-06-01 Epub Date: 2026-02-11 DOI: 10.1016/j.bios.2026.118511

Minjie Yang , Meina Feng , Ling Zha , Xi Liu , Yuanhui Ma , Ying Huang , Ruizhuo Ouyang , Dong Sun , Yuqing Miao , Baolin Liu

{"title":"Dual-cycle signal amplification on an Au@CeO2-x/Bi2MoO6 platform: Toward an ultrasensitive and enzyme-free sandwich immunosensor for CA125 in clinical diagnostics","authors":"Minjie Yang , Meina Feng , Ling Zha , Xi Liu , Yuanhui Ma , Ying Huang , Ruizhuo Ouyang , Dong Sun , Yuqing Miao , Baolin Liu","doi":"10.1016/j.bios.2026.118511","DOIUrl":"10.1016/j.bios.2026.118511","url":null,"abstract":"<div><div>The early detection of ovarian cancer biomarkers is essential for effective diagnosis, calling for highly sensitive and reliable immunosensing platforms. In this work, we present a dual-cycle amplified sandwich-type electrochemical immunosensor for the detection of Carbohydrate Antigen 125 (CA125), a key biomarker of ovarian cancer. The sensing platform was fabricated by immobilizing the primary antibody (Ab1) on a glassy carbon electrode modified with Au@GO-Chi-Fc nanocomposite. A catalytic probe was prepared by conjugating the secondary antibody (Ab2) with gold nanoparticles anchored on Ce-doped Bi<sub>2</sub>MoO<sub>6</sub> nanoflowers (Au@CeO<sub>2-x</sub>/Bi<sub>2</sub>MoO<sub>6</sub>). These probes efficiently catalyze the reduction of p-nitrophenol (p-NP) to p-aminophenol (p-AP), which subsequently triggers an electrocatalytic chemical (ECC) redox cycling reaction with NaBH<sub>4</sub>, leading to significant signal amplification. This enzyme-free strategy provides the immunosensor with an ultra-wide linear range (0.0005–100 U mL<sup>−1</sup>) and an ultralow detection limit (0.00049 U mL<sup>−1</sup>), enabling accurate quantification of CA125 in diverse human serum samples. Moreover, the immunosensor demonstrates high selectivity, excellent reproducibility, and stable performance over 18 days, underscoring its robustness and clinical potential for the detection of trace CA125 in complex biological matrices.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118511"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in peptide nucleic acid-based sensors and antisense therapeutics: Surface immobilization on planar gold substrates 肽核酸传感器和反义疗法的研究进展：平面金底物表面固定化

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-06-01 Epub Date: 2026-02-03 DOI: 10.1016/j.bios.2026.118481

Birgit Meindl , Katharina Pfennigbauer , Agnes Lenz, Stephanie Müllauer, Brigitte Holzer

{"title":"Advances in peptide nucleic acid-based sensors and antisense therapeutics: Surface immobilization on planar gold substrates","authors":"Birgit Meindl , Katharina Pfennigbauer , Agnes Lenz, Stephanie Müllauer, Brigitte Holzer","doi":"10.1016/j.bios.2026.118481","DOIUrl":"10.1016/j.bios.2026.118481","url":null,"abstract":"<div><div>Peptide nucleic acids (PNAs) are synthetic analogues of nucleic acids featuring a neutral peptide-backbone that provides exceptional stability and resistance to enzymatic degradation. They are highly specific towards complementary DNA or RNA sequences rendering them ideal capture probes for sensitive and selective biosensing. The uncharged backbone minimizes nonspecific interactions and reduces background noise in bioelectronic devices, thereby improving signal-to-noise ratios. The precise immobilization of PNAs on gold substrates allows the development of real-time, label-free electrochemical and optical sensors. Parameters – such as immobilization strategy, concentration, temperature, substrate morphology – that strongly influences the surface probe density and ultimately play key roles in the sensor's performance are discussed within this review.</div><div>Furthermore, this review highlights current strategies for tuning planar gold-based PNA sensors and outlines how nucleic acid sensor technologies can be leveraged to evaluate emerging PNA-based antisense oligonucleotides. Given the relevance of PNA derivatives in therapeutic oligonucleotide development, it further shows how sensor platforms can be used to assess and improve the design and binding kinetics of new PNA antisense drug candidates.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118481"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ mapping of HSA reabsorption by a NIR fluorogenic sensor as a practical phenotypic assay for nephroprotective drug discovery 近红外荧光传感器原位定位HSA重吸收作为肾保护药物发现的实用表型分析

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-06-01 Epub Date: 2026-02-05 DOI: 10.1016/j.bios.2026.118490

Yufan Fan , Bei Zhao , Ya Zhang , Pingjin Xie , Lele Liu , Xuerui Wang , Guanghao Zhu , Zhaobin Guo , Fangyuan Wang , Qiang Jin , Jing Hu , Guangbo Ge

{"title":"In situ mapping of HSA reabsorption by a NIR fluorogenic sensor as a practical phenotypic assay for nephroprotective drug discovery","authors":"Yufan Fan , Bei Zhao , Ya Zhang , Pingjin Xie , Lele Liu , Xuerui Wang , Guanghao Zhu , Zhaobin Guo , Fangyuan Wang , Qiang Jin , Jing Hu , Guangbo Ge","doi":"10.1016/j.bios.2026.118490","DOIUrl":"10.1016/j.bios.2026.118490","url":null,"abstract":"<div><div>The impaired reabsorption of kidney tubules is a critical early event in drug-induced kidney injury (DIKI), making real-time monitoring of human serum albumin (HSA) endocytosis essential for assessing renal function and screening nephroprotective agents. Inspired by the specific warfarin-HSA interactions, a novel coumarin-derived near-infrared (NIR) fluorogenic sensor, <strong>NCBP</strong>, was engineered based on a twisted intramolecular charge transfer (TICT) mechanism. Under physiological conditions, <strong>NCBP</strong> rapidly and stably bound to subdomain IIA of HSA with 1:1 stoichiometry, triggering a 79-fold fluorescence enhancement at 665 nm. <strong>NCBP</strong> integrated multiple advantages for detecting HSA, including instantaneous response, high physicochemical stability, ultrahigh sensitivity, and excellent selectivity. Furthermore, <strong>NCBP</strong> enabled <em>in situ</em> spatiotemporal imaging of HSA reabsorption in living nephrocytes and renal tissues, which was then used for screening nephroprotective agents to mitigate cisplatin-induced nephrotoxicity (CINT). Nobiletin (NOB) and 4’,7-dimethoxyisoflavone (DIF) were identified as promising lead compounds capable of restoring renal uptake function and mitigating cisplatin-induced oxidative stress. In CINT mice, both NOB and DIF significantly improved renal function and attenuated tubular injury, confirming their nephroprotective potentials. Collectively, a TICT-based NIR sensor was developed for <em>in situ</em> imaging of HSA reabsorption, offering a robust phenotypic platform for spatiotemporal mapping of cellular HSA trafficking and discovering nephroprotective agents.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118490"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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