Biosensors and Bioelectronics最新文献_第3页

Smartphone-deployable and all-in-one machine vision for visual quantification analysis based on distance readout of electrophoresis titration biosensor 基于电泳滴定生物传感器距离读数的可部署智能手机的一体化机器视觉视觉定量分析。

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-10-02 DOI: 10.1016/j.bios.2024.116832

{"title":"Smartphone-deployable and all-in-one machine vision for visual quantification analysis based on distance readout of electrophoresis titration biosensor","authors":"","doi":"10.1016/j.bios.2024.116832","DOIUrl":"10.1016/j.bios.2024.116832","url":null,"abstract":"<div><div>As a class of point-of-care (POC) assays with visible distance readout (thermometer style), the electrophoresis titration (ET) biosensor affords high robustness, versatility, and simplicity for point-of-care quantification. However, naked-eye observation of the distance readout is unreliable in POC settings and manual processing of distance readout is time-consuming. Herein, we developed a smartphone-deployable and all-in-one machine vision for four ET biosensors (bovine serum albumin, melamine, uric acid, glutathione) to classify and quantify the samples simultaneously. To ensure accurate and rapid quantification on the smartphone, we customized the decolorization methods and edge detection operators to balance the region of interest (ROI) extraction performance and processing speed. We then established a dataset of 180 distance readout images to endow our machine vision with the ability to classify four sample types. Consequently, our machine vision demonstrated high accuracy in determining the sample type (>97.2%) and concentration (>97.3%). Moreover, expanding its applications to other targets was readily achieved by including distance readout images of other ET biosensors (e.g., hemoglobin A1c) in the dataset. Therefore, our strategy of constructing machine vision is compatible with the versatile ET biosensor technique, suggesting that the same strategy can be used for other thermometer-style POC assays.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Longitudinal monitoring of hypertonia through a multimodal sensing glove 通过多模态传感手套对高张力症进行纵向监测。

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-10-02 DOI: 10.1016/j.bios.2024.116829

引用次数: 0

Energy stress induced cardiac autophagy detection via a chronic and dynamic cardiomyocytes-based biosensing platform 通过基于心肌细胞的慢性动态生物传感平台检测能量应激诱导的心脏自噬。

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-10-01 DOI: 10.1016/j.bios.2024.116821

{"title":"Energy stress induced cardiac autophagy detection via a chronic and dynamic cardiomyocytes-based biosensing platform","authors":"","doi":"10.1016/j.bios.2024.116821","DOIUrl":"10.1016/j.bios.2024.116821","url":null,"abstract":"<div><div>Hypoglycemia is a common complication which occurs during the treatment of diabetes, closely associated with cardiovascular events. A sudden decrease in blood glucose increases the risk of arrhythmia, which can lead to sudden cardiac death. This event is usually accompanied by abnormal electrophysiological activities in cardiomyocytes. However, traditional models do not efficiently reflect real-time cardiomyocyte electrophysiological changes under various glucose deprivation conditions in a large-scale and high-throughput manner. Therefore, we need to develop a new biosensing platform to aid in related scientific research. In this study, a cardiomyocyte-based biosensor was developed for real-time, noninvasive monitoring of the electrophysiological responses of cardiomyocytes under different glucose concentrations. The findings show that low-glucose conditions result in abnormal electrophysiology in cardiomyocytes, but autophagy enables cells to survive this adversity. Inhibition of autophagy exacerbates electrophysiological abnormalities, and long-term glucose starvation causes irreversible damage to cardiomyocytes. The proposed chronic and dynamic cardiomyocyte-based biosensing platform provides a new tool for understanding the effects of hypoglycemia on the in vitro cardiomyocyte-based heart model, revealing that autophagy has the potential to be an alternative treatment for diabetes and hypoglycemia.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142379698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative detection of circular RNA and microRNA at point-of-care using droplet digital CRISPR/Cas13a platform 利用液滴数字 CRISPR/Cas13a 平台在护理点定量检测环状 RNA 和 microRNA。

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-10-01 DOI: 10.1016/j.bios.2024.116825

{"title":"Quantitative detection of circular RNA and microRNA at point-of-care using droplet digital CRISPR/Cas13a platform","authors":"","doi":"10.1016/j.bios.2024.116825","DOIUrl":"10.1016/j.bios.2024.116825","url":null,"abstract":"<div><div>Circular RNA (circRNA) and microRNA (miRNA) are both non-coding RNAs (ncRNAs) that serve as biomarkers for cancer diagnosis and prognosis. Quantitative detection of these ncRNAs is of particular importance to elucidate the functional mechanisms and evaluate their potential as biomarkers. However, the inherent structures of circRNA and miRNA are different from the mRNA, conventional qRT-PCR is unsuitable for the detection of these ncRNAs. Here, we propose a sensitive method for quantitative detection of circRNA and miRNA using polydisperse droplet digital CRISPR/Cas13a (PddCas13a). It can achieve limits of detection (LOD) as low as ∼10 aM without polymerase-based amplification. To efficiently detect the circRNA and miRNA in real samples, we use a chemically modified crRNA to enhance the stability of crRNA and improve the performance of Cas13a in complex environments containing contaminants. By integrating an extraction-free procedure with PddCas13a, we experimentally demonstrate the applicability of PddCas13a by testing clinical samples. Furthermore, we develop an automated and portable instrument for PddCas13a and verify its applicability for the detection of circRNA and miRNA from exosomes in point-of-care (POC) setting. This is the first report to detect the circRNA and miRNA simultaneously in POC setting. We envision this platform could promote the research of ncRNAs.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142379701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel platform for mutation detection in colorectal cancer using a PNA-LNA molecular switch 利用 PNA-LNA 分子开关检测结直肠癌突变的新型平台

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-10-01 DOI: 10.1016/j.bios.2024.116813

{"title":"A novel platform for mutation detection in colorectal cancer using a PNA-LNA molecular switch","authors":"","doi":"10.1016/j.bios.2024.116813","DOIUrl":"10.1016/j.bios.2024.116813","url":null,"abstract":"<div><div>Detection of <em>KRAS</em> mutation in colorectal cancer (CRC) is important in the prediction of response to target therapy. The study aims to develop a novel mutation detection platform called the “PNA-LNA molecular switch” for the detection of <em>KRAS</em> mutation in CRC. We employed the enhanced binding specificity of peptide nucleic acid (PNA) and locked nucleic acid (LNA) in conjunction with a loop-mediated isothermal amplification (LAMP) approach to identify the mutation status of <em>KRAS</em> oncogene codon 12 (c.35G>T/G12V and c.35G>A/G12D) using synthetic oligonucleotides and colon cancer cell lines (Caco-2 and SW480). This method specifically blocked the amplification of the wild-type sequences while substantially amplifying the mutated ones, which was visualized by both colorimetric and fluorescence assays. We then checked the mutation profile of <em>KRAS</em> codon 12 in the DNA derived from tumor tissue samples (number of samples, <em>n</em> = 30) and circulating tumor cells (<em>n</em> = 24) from CRC patients. Finally, we validated the results by comparing them with the data obtained from DNA sequencing of colon tumors (<em>n</em> = 21) of the same CRC patients. This method showed excellent sensitivity (1 DNA copy/μl), reproducibility [relative standard deviation (%RSD) < 5%, for n = 3], and linear dynamic range (1 ag/μl-10 pg/μl, R<sup>2</sup> = 0.94). This platform is significantly faster, relatively cheaper, has superior sensitivity and specificity, and does not require any high-end equipment. To conclude, this method has the potential to be translated into clinical settings for the detection of mutations in diverse diseases and conditions.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142359112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Target-mediated silver deposition-based electrochemical biosensor for highly sensitive detection of human chorionic gonadotropin 高灵敏度检测人绒毛膜促性腺激素的靶向介导银沉积电化学生物传感器。

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-10-01 DOI: 10.1016/j.bios.2024.116830

{"title":"Target-mediated silver deposition-based electrochemical biosensor for highly sensitive detection of human chorionic gonadotropin","authors":"","doi":"10.1016/j.bios.2024.116830","DOIUrl":"10.1016/j.bios.2024.116830","url":null,"abstract":"<div><div>As a glycoprotein hormone, human chorionic gonadotropin (hCG) is an established marker for pregnancy test. On the basis of the target-mediated silver deposition (TSD), in this work, we report the development of an amplification-free electrochemical biosensor for the highly sensitive detection of hCG. The detection of hCG involves the use of the affinity peptide-modified electrode for hCG capture (the CGGSSPPLRINRHILTR peptide containing the hCG-binding domain of the PPLRINRHILTR sequence is used as the affinity peptide), the oxidation of the diol sites of the glycan chains on hCG hormones into aldehyde groups by NaIO<sub>4</sub>, and the deposition of silver nanoparticles (AgNPs) for the solid-state voltammetric stripping analysis. Due to the deposition of multiple AgNPs while the solid-state Ag/AgCl voltammetric process has a high signal-to-noise ratio, the TSD-based electrochemical biosensor can be applied to the highly sensitive detection of hCG without the need for signal amplification. Under optimal conditions, the stripping current increased linearly with an increasing hCG concentration over the range from 1.0 to 25 mIU/mL, with a detection limit of 0.45 mIU/mL. Owing to the high specificity of the hCG-binding peptide PPLRINRHILTR, this electrochemical hCG biosensor exhibits high selectivity. The results of the quantitative assay of hCG in urine samples at the concentrations of 25, 10, and 1.0 mIU/mL are desirable, indicating the good anti-interference capability. As the TSD-based electrochemical biosensor allows the amplification-free detection of low-abundance hCG, it is easy to use and cost-effective, showing great promise in point-of-care assay of hCG for pregnancy test.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzyme-accelerated catalytic DNA circuits enable rapid and one-pot detection of bacterial pathogens 酶加速催化 DNA 电路可实现对细菌病原体的快速和一次性检测。

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-10-01 DOI: 10.1016/j.bios.2024.116822

{"title":"Enzyme-accelerated catalytic DNA circuits enable rapid and one-pot detection of bacterial pathogens","authors":"","doi":"10.1016/j.bios.2024.116822","DOIUrl":"10.1016/j.bios.2024.116822","url":null,"abstract":"<div><div>Catalytic DNA circuits, serving as signal amplification strategies, can enable simple and accurate detection of pathogenic bacteria in complex matrices but suffer from low reaction rates and depths. Herein, we design an enzyme-accelerated catalytic hairpin assembly (EACHA) in which duplex DNA products are converted into hairpin reactants to continue participating in the next circuit reaction with the assistance of RNase H. Profiting from the high recyclability of the reactants, EACHA exhibits an approximately 37.6-fold enhancement in the rate constant and a two-order-of-magnitude improvement in sensitivity compared to conventional catalytic hairpin assembly (CHA). By integrating an allosteric probe with EACHA, a one-pot method is developed for rapid and direct detection of <em>S. enterica</em> Enteritidis (<em>S.</em> Enteritidis)<em>.</em> This method is capable of detecting 15 CFU mL<sup>−1</sup> of <em>S.</em> Enteritidis within 20 min, which is superior to that of real-time PCR. By testing 60 milk samples, we demonstrate this method's high accuracy in discriminating contaminated samples, with an area under the curve (AUC) of 0.997. Moreover, this method can be employed to accurately diagnose early-stage infected mice, with an AUC of 1.00 for feces samples and 0.986 for serum samples. Therefore, this study offers a simple and feasible method for identifying pathogens in complex matrices.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flexible tactile sensors inspired by bio-mechanoreceptors 受生物机械感受器启发的柔性触觉传感器

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-10-01 DOI: 10.1016/j.bios.2024.116828

{"title":"Flexible tactile sensors inspired by bio-mechanoreceptors","authors":"","doi":"10.1016/j.bios.2024.116828","DOIUrl":"10.1016/j.bios.2024.116828","url":null,"abstract":"<div><div>Mechanoreceptors in animals and plants play a crucial role in sensing mechanical stimuli such as touch, motion, stretch, and vibration. Learning from the mechanisms of mechanoreceptors may facilitate the development of bionic tactile sensors, leading to higher sensitivity, spatial resolution, and dynamic ranges. However, very little literature has comprehensively discussed the relevance of biological tactile sensing systems and machine-learning-based bionic tactile sensors. This review first introduces the structural features, signal acquisition and transmission mechanisms, and feedback processes of both plant and animal mechanoreceptors, and then summarizes the efforts to develop bionic tactile sensors by mimicking the morphologies and structures of mechanoreceptors in plants and animals. Additionally, the integration of artificial intelligence approaches with these sensors for data processing and analysis are demonstrated, followed by the perspectives on current challenges and future trends in bionic tactile sensors. This review addresses the challenges in developing high-performance tactile sensors by focusing on surface microstructures and biological mechanoreceptors, serving as a valuable reference for developing bionic tactile sensors with enhanced sensitivity and multimodal sensing capabilities. Furthermore, it may benefit the future development of smart sensing systems integrated with artificial intelligence for more precise object and texture recognition.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Triple-Mismatch Differentiating assay exploiting activation and trans cleavage of CRISPR-Cas12a for mutation detection with ultra specificity and sensitivity 利用 CRISPR-Cas12a 的激活和反式裂解进行突变检测的三重错配分化测定，具有超特异性和灵敏度。

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-09-30 DOI: 10.1016/j.bios.2024.116826

{"title":"A Triple-Mismatch Differentiating assay exploiting activation and trans cleavage of CRISPR-Cas12a for mutation detection with ultra specificity and sensitivity","authors":"","doi":"10.1016/j.bios.2024.116826","DOIUrl":"10.1016/j.bios.2024.116826","url":null,"abstract":"<div><div>Liquid biopsy technology is non-invasive and convenient, and is currently an emerging technology for cancer screening. Among them, clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 12a (Cas12a) based nucleic acid detection technology has the advantages of high sensitivity, rapidity, and easy operation. However, CRISPR-Cas12a does not discriminate single-base mismatches of targets well enough to meet the needs of clinical detection. Herein, we developed the Triple-Mismatch Differentiating (TMD) assay. This assay amplified the small thermodynamic difference in mismatches at one site at the level of CRISPR-Cas12a activation to a significant thermodynamic difference at three sites at both the level of CRISPR-Cas12a activation and trans-cleavage, which greatly improves the ability of CRISPR-Cas12a to discriminate between base mismatches. Our manipulation greatly improved the specificity of the CRISPR-Cas12a system while maintaining its inherent sensitivity and simplicity, increasing the detection limit to 0.0001%. When testing samples from pancreatic cancer patients, our results were highly consistent with NGS sequencing results. We believe that the TMD assay will provide a new technology for early cancer detection and will be widely used in the clinical practice.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142379697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel cleanroom-free technique for simultaneous electrodeposition of polypyrrole onto array of IDuEs: Towards low-cost, stable and accurate point-of-care TBI diagnosis without trained manpower 在 IDuE 阵列上同时电沉积聚吡咯的新型无尘室技术：无需训练有素的人员即可实现低成本、稳定和准确的创伤性脑损伤床旁诊断。

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-09-30 DOI: 10.1016/j.bios.2024.116824

{"title":"A novel cleanroom-free technique for simultaneous electrodeposition of polypyrrole onto array of IDuEs: Towards low-cost, stable and accurate point-of-care TBI diagnosis without trained manpower","authors":"","doi":"10.1016/j.bios.2024.116824","DOIUrl":"10.1016/j.bios.2024.116824","url":null,"abstract":"<div><div>Drop-casted polypyrrole (PPY) nanomaterial-based point-of-care Traumatic Brain Injury (TBI) immunosensing platforms reported previously demand trained manpower at field-test, due to poor adhesion between nanomaterial and electrode surface, limiting the point-of-care purpose. The usage of conventional clean-room-based physical and chemical vapor deposition techniques in creating strong adhesion is limited on account of cost and process complexity. Addressing this technical gap, we report a novel low-cost clean-room-free technique that can effectively electrodeposit the PPY simultaneously onto the working areas of array of Interdigitated microelectrodes (IDμEs) from the precursor solution. Through optimization of deposition cycles and molar concentration ratio of monomer and oxidizing agents, a high-quality nanomaterial was electrodeposited on IDμEs' surface. Further, by using the electrodeposited PPY as a bioelectrical transducer, the TBI-specific UCHL1 and GFAP target analytes were simultaneously detected in terms of variation of DC-Resistance and AC-Capacitance parameters, recorded through chemiresistive I-V and chemicapacitive C-F responses of bioelectrodes, respectively. Such simultaneous multianalyte-detection in terms of multiple parameters increases the diversity of decision-making parameters by several folds, inherently aids in enhancing the diagnostic accuracy of TBI test kit. Here, the efficiency of the electrodeposited PPY-based chemiresistive and chemicapacitive immunosensing platforms in detecting TBI-specific target analytes simultaneously in real-time human-plasma samples was analyzed in terms of sensitivity, resolution, LoD, RoD, long-term stability (30 weeks), and the same is compared with drop-cast PPY-based immunosensing platform. Notably, the electrodeposited PPY sensing platforms showed superior performance in terms of sensitivity, LoD, device variability and long-term stability without demanding any trained manpower in the field.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0