Biosensors and Bioelectronics最新文献_第3页

Probing the stability of Hg2+-mediated self-duplexes: Effects of thymine residues position and number via a single-molecule protein nanopore sensing technique 通过单分子蛋白质纳米孔传感技术探测Hg2+介导的自双链的稳定性：胸腺嘧啶残基位置和数量的影响。

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-05-30 DOI: 10.1016/j.bios.2025.117626

Isabela Dragomir, Irina Schiopu, Alina Asandei

{"title":"Probing the stability of Hg2+-mediated self-duplexes: Effects of thymine residues position and number via a single-molecule protein nanopore sensing technique","authors":"Isabela Dragomir, Irina Schiopu, Alina Asandei","doi":"10.1016/j.bios.2025.117626","DOIUrl":"10.1016/j.bios.2025.117626","url":null,"abstract":"<div><div>In this study, we report for the first time that mercury metal ions (Hg<sup>2+</sup>) can stabilize self-duplexes that would otherwise fail to hybridize. We designed two short DNA fragments containing varying numbers of thymine residues, positioned either in the central region or at the terminal ends of the resulting double-stranded structures formed via non-canonical T-Hg<sup>2+</sup>-T base pairs. The stability and structural properties of these newly formed duplexes were investigated using single-molecule nanopore electrophysiology. Our results demonstrated that duplexes with metal-coordinated base pairs located in the central region could be unzipped with relative ease, whereas those with terminal T-Hg<sup>2+</sup>-T pairs exhibited remarkable structural rigidity, resisting modification even under high external force. We emphasize that both the position and the number of thymine residues within the DNA strands are critical key players in the hybridization efficiency and the stability of duplexes. This novel coordination chemistry approach holds significant potential for the development of highly responsive, label-free nanopore sensors for metal ions, the facilitation of strand self-recognition, and the precise detection of thymine positioning within DNA duplexes.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"285 ","pages":"Article 117626"},"PeriodicalIF":10.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanozymes enhancing field-effect transistor nanosensor for in-situ monitoring intracellular hydrogen peroxide release 纳米酶增强场效应晶体管纳米传感器用于原位监测细胞内过氧化氢释放

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-05-30 DOI: 10.1016/j.bios.2025.117643

Guowu Liang , Luyao Yang , Ling Xiao , Lina Tang , Shibo Cheng , Zhongyue Sun , Yulin Zhang , Fan Yang , Guo-Jun Zhang

{"title":"Nanozymes enhancing field-effect transistor nanosensor for in-situ monitoring intracellular hydrogen peroxide release","authors":"Guowu Liang , Luyao Yang , Ling Xiao , Lina Tang , Shibo Cheng , Zhongyue Sun , Yulin Zhang , Fan Yang , Guo-Jun Zhang","doi":"10.1016/j.bios.2025.117643","DOIUrl":"10.1016/j.bios.2025.117643","url":null,"abstract":"<div><div>Field-effect transistor (FET) nanodevices are widely recognized as highly sensitive sensors for continuous detection of bioactive molecules like hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). However, accurate and real-time monitoring of H<sub>2</sub>O<sub>2</sub> poses challenges due to its instability and low concentration in organisms. To address these challenges, we construct an enhanced FET by one-step interfacing with nanozymes that possess natural enzyme-like catalytic properties and exceptional stability. Specifically, reduced graphene oxide (RGO) is drop-casted onto the fabricated FET channel, after which the nanozymes are assembled with RGO through π-π stacking interactions. The nanozyme-functionalized FET sensor is able to realize continuous H<sub>2</sub>O<sub>2</sub> monitoring, featuring a broad linear detection range (1 pM–10 nM), an ultralow detection limit (0.5 pM), high selectivity, and rapid response. Moreover, the sensor enables real-time monitoring of intracellular H<sub>2</sub>O<sub>2</sub> release from cells cultured within the FET channel, demonstrating significant potential for distinguishing between cancer cells and normal cells. In addition, the sensor successfully tracks the dynamic regulation of intracellular H<sub>2</sub>O<sub>2</sub> efflux under drug stimulation. This platform combines high sensitivity with excellent biocompatibility, making it highly promising for applications in cell metabolism research, disease diagnosis, and drug efficacy evaluation and screening.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"286 ","pages":"Article 117643"},"PeriodicalIF":10.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144190474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A smartphone-enabled colorimetric tumor-derived exosomes sensing based on multi-enzyme catalysis and dual-recognition triggered CRISPR/Cas12a trans-cleavage 基于多酶催化和双识别触发CRISPR/Cas12a反式切割的智能手机激活肿瘤衍生外泌体比色检测

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-05-30 DOI: 10.1016/j.bios.2025.117644

Jing Jiang, Xianwen Kan

{"title":"A smartphone-enabled colorimetric tumor-derived exosomes sensing based on multi-enzyme catalysis and dual-recognition triggered CRISPR/Cas12a trans-cleavage","authors":"Jing Jiang, Xianwen Kan","doi":"10.1016/j.bios.2025.117644","DOIUrl":"10.1016/j.bios.2025.117644","url":null,"abstract":"<div><div>Detection of protein profiling on exosomes exhibits great promise for early non-invasive and accurate diagnosis of tumor in clinical diagnostics. However, it still faces multiple challenges, such as expensive instruments requirement and weak specificity by single biomarker. Herein, based on a dual-recognition strategy, a ternary hybrid of a trigger DNA (TDNA), EpCAM aptamer, and CD63 aptamer was used to capture A549 cells-derived exosomes to release TDNA, which initiated the trans-cleavage activity of CRISPR/Cas12a to nonspecifically cleave single-stranded DNA (ssNDA) and then resulted in the isolating of ssDNA linked nanozyme of Zr/Fe-CeO<sub>2</sub>@Ir@CaO<sub>2</sub>@HA (ZFCIrCH). ZFCIrCH not only achieved H<sub>2</sub>O<sub>2</sub>/O<sub>2</sub> self-supply, but also possessed high peroxidase-like, oxidase-like, and superoxide dismutase-like activities, thereby generating a sensitive colorimetric signal for A549 cells-derived exosomes detection with a low limit of detection (LOD) of 31 particles/mL. Using a smartphone to analyze colorimetric images, exosome concentration also can be precisely quantified with a LOD of 29 particles/mL, which could also successfully distinguish healthy people from lung cancer patients. With the advantages of high sensitivity, good specificity, low cost, and convenient on-site detection of tumor-derived exosomes, the present colorimetric sensor has great promise in the accurate diagnosis of diseases.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"286 ","pages":"Article 117644"},"PeriodicalIF":10.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Electrochemiluminescence immunosensor based on terbium metal-organic gel emitter and CoMnO3 electrocatalyst for the sensitive detection of N-terminal pro-brain natriuretic peptide 基于金属铽-有机凝胶发射器和CoMnO3电催化剂的电化学发光免疫传感器用于脑钠肽n端前体的灵敏检测

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-05-30 DOI: 10.1016/j.bios.2025.117642

Jing Li, Shenglan Hu, Lingling Zheng, Lixin Xu, Yusheng Wu, Biyang Deng

{"title":"An Electrochemiluminescence immunosensor based on terbium metal-organic gel emitter and CoMnO3 electrocatalyst for the sensitive detection of N-terminal pro-brain natriuretic peptide","authors":"Jing Li, Shenglan Hu, Lingling Zheng, Lixin Xu, Yusheng Wu, Biyang Deng","doi":"10.1016/j.bios.2025.117642","DOIUrl":"10.1016/j.bios.2025.117642","url":null,"abstract":"<div><div>The construction of a sensitive electrochemiluminescence (ECL) immunosensor for the detection of N-terminal pro-brain natriuretic peptide (NT-proBNP) is demonstrated in this paper using terbium metal-organic gel (Tb-MOG) as an ECL emitter. Firstly, stable ECL signal was obtained using the easily synthesized Tb-MOG as the emitter and persulfate (S<sub>2</sub>O<sub>8</sub><sup>2−</sup>) as the co-reactant. Secondly, the reduction of S<sub>2</sub>O<sub>8</sub><sup>2−</sup> was catalyzed by the introduction of CoMnO<sub>3</sub> nanoflower as a co-reaction promoter, which further enhanced the ECL intensity. When mixed-valence transition metal ions (Co<sup>3+</sup>/Co<sup>2+</sup> and Mn<sup>4+</sup>/Mn<sup>3+</sup>) were reversibly transformed in CoMnO<sub>3</sub>, the production of sulfate radical anion (SO<sub>4</sub><sup>•–</sup>) was greatly enhanced. Additionally, the unique nanoflower structure of CoMnO<sub>3</sub> possesses a large specific surface area, enhancing its catalytic performance. Thanks to the well-established sensing strategy, the resultant ECL immunosensor exhibited satisfactory performance in accurately detecting NT-proBNP, with a linear concentration range of 10 fg/mL to 100 ng/mL and a detection limit of 2.1 fg/mL, offering great promise for clinical translation and broader heart failure diagnosis.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117642"},"PeriodicalIF":10.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144205256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultra-stable biosensor transducer for continuous monitoring 用于连续监测的超稳定生物传感器传感器

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-05-28 DOI: 10.1016/j.bios.2025.117638

Darya V. Vokhmyanina, Olesya E. Sharapova, Arkady A. Karyakin

{"title":"Ultra-stable biosensor transducer for continuous monitoring","authors":"Darya V. Vokhmyanina, Olesya E. Sharapova, Arkady A. Karyakin","doi":"10.1016/j.bios.2025.117638","DOIUrl":"10.1016/j.bios.2025.117638","url":null,"abstract":"<div><div>Prussian Blue (PB), being the most active and selective hydrogen peroxide transducer and allowing H<sub>2</sub>O<sub>2</sub> detection by its reduction in the presence of oxygen, suffers from solubilization by the product of electrode reaction, hydroxyl ion (OH‾). Stabilization of the transducer is possible with isostructural nickel hexacyanoferrate (NiHCF). Herewith we report on the new composite PB-NiHCF material synthesized, which is noticed by SEM imaging. Reliable calibration-free H<sub>2</sub>O<sub>2</sub> monitoring with the corresponding modified electrode is up to 100 times longer than in case of pure PB and up to 10 times – than with the electrodes stabilized through layer-by-layer assembly. The PB-NiHCF composite film displays operational stability, which is even higher as compared to the stabilizer itself (NiHCF thick film, 30 nmol cm<sup>−2</sup>), but is characterized by the activity close to it of pure Prussian Blue. The corresponding biosensor is completely stable during 3 days of continuous glucose (5 mM) monitoring. The reported ultra-stable and highly sensitive transducer would provide reliable continuous monitoring of low-molecular weight metabolites.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"286 ","pages":"Article 117638"},"PeriodicalIF":10.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144184323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a multi-miRNA detection platform for enhanced diagnostics of colorectal cancer 一种多mirna检测平台的开发，用于增强结直肠癌的诊断

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-05-28 DOI: 10.1016/j.bios.2025.117639

Bo Zhou , Tingting Fan , Hui Chen , Yiming Huang , Xinjie Lai , Shanshan Feng , Zijian Ding , Yan Chen , Yuyang Jiang

{"title":"Development of a multi-miRNA detection platform for enhanced diagnostics of colorectal cancer","authors":"Bo Zhou , Tingting Fan , Hui Chen , Yiming Huang , Xinjie Lai , Shanshan Feng , Zijian Ding , Yan Chen , Yuyang Jiang","doi":"10.1016/j.bios.2025.117639","DOIUrl":"10.1016/j.bios.2025.117639","url":null,"abstract":"<div><div>The identification of specific miRNA biomarkers has considerably advanced colorectal cancer (CRC) diagnostics. Our prior research identified four key miRNA signatures—miR-10b-5p, miR-130a-3p, miR-561–5p, and miR-4684-5p—as potential diagnostic markers for CRC. To enhance the clinical utility of these biomarkers, we developed a novel multi-miRNA detection platform, RCA-SDA-CRISPR (RS-CRISPR), which integrates rolling circle amplification (RCA), strand displacement amplification (SDA), and CRISPR/Cas12a technologies. This platform employs RCA products as SDA templates, maximizes miRNA binding sites, and utilizes the spatial arrangement of miRNAs to bypass limitations of traditional enzymatic cleavage, enabling rapid, simultaneous detection of multiple miRNAs. RS-CRISPR achieved a detection sensitivity of 57.8fM for target miRNAs. In clinical validation, this method successfully distinguished tumor tissue from adjacent non-cancerous tissue in five patients with CRC. In addition, during the serum analysis of 12 normal controls (NC) and 12 CRC patients, we found that the levels of four specific miRNAs were significantly higher in CRC patients compared to NC (<em>p</em> = 0.00646), underscoring its diagnostic potential. These findings establish RS-CRISPR as a promising, precise, and efficient diagnostic tool for clinical CRC management through multi-miRNA detection.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117639"},"PeriodicalIF":10.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioinspired imprinted nanoprobes for near-infrared fluorescence recognition and detection of biogenic amines 生物印迹纳米探针用于近红外荧光识别和检测生物胺

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-05-27 DOI: 10.1016/j.bios.2025.117636

Xuecheng Zhu, Yuzhen Wang, Dianwei Zhang, Huilin Liu, Baoguo Sun

{"title":"Bioinspired imprinted nanoprobes for near-infrared fluorescence recognition and detection of biogenic amines","authors":"Xuecheng Zhu, Yuzhen Wang, Dianwei Zhang, Huilin Liu, Baoguo Sun","doi":"10.1016/j.bios.2025.117636","DOIUrl":"10.1016/j.bios.2025.117636","url":null,"abstract":"<div><div>Histamine (HA) is a critical biomarker for food spoilage, necessitating its rapid and sensitive detection. In this study, inspired by the red shift observed in yellow fluorescent protein due to π-π stacking and the enhanced fluorescence of the green fluorescent protein mutant BFPms1 resulting from Zn<sup>2+</sup>-induced structural rigidification, peptide nanoparticles (PNPs) with near-infrared fluorescence were developed by self-assembling tri-histidine peptides with Zn<sup>2+</sup>. These PNPs exhibit excellent optical properties and biocompatibility, making them suitable as fluorescent nanoprobes for biological and food-related applications. Using HA as a template molecule, the PNPs functioned both as carrier and fluorescent signal sources for the synthesis of molecularly imprinted polymers (PNPs@MIPs), yielding materials with abundant, highly selective recognition sites. The PNPs@MIPs demonstrated sensitive and accurate responses to HA within a concentration range of 1–500 mg/L, with a detection limit of 0.828 mg/L, leveraging the retained near-infrared fluorescence. The precisely engineered imprinted cavities enabled rapid and selective HA capture, achieving a response time of 90 s and an Imprinting Factor of 8.34. Furthermore, a smartphone-integrated sensing platform allowed for visual, mobile HA detection through color changes, facilitating on-site analysis. This biomimetic strategy offers a promising route for the development of high-performance nanomaterials for targeted sensing, with broad implications for public health and food safety.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"286 ","pages":"Article 117636"},"PeriodicalIF":10.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144178609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasensitive quantification of aflatoxin B1 via nanobody phage -mediated quantitative PCR and digital PCR for agro-products 利用纳米噬菌体介导的定量PCR和数字PCR对农产品中黄曲霉毒素B1的超灵敏定量

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-05-27 DOI: 10.1016/j.bios.2025.117622

Junjie Zeng , Yanping Li , Qinghua He , Ji Xia , Jiebiao Guo , Zhui Tu

{"title":"Ultrasensitive quantification of aflatoxin B1 via nanobody phage -mediated quantitative PCR and digital PCR for agro-products","authors":"Junjie Zeng , Yanping Li , Qinghua He , Ji Xia , Jiebiao Guo , Zhui Tu","doi":"10.1016/j.bios.2025.117622","DOIUrl":"10.1016/j.bios.2025.117622","url":null,"abstract":"<div><div>Phage display-mediated immuno-polymerase chain reaction (PD-IPCR) combines the strengths of phage display technology and immuno-PCR. The link between genotype and phenotype has been established by displaying antibody fragments on the surface of phage particles, allowing signal transmission. In this study, we utilized aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) as a model system to investigate the efficacy of PD-IPCR. Signal amplification was achieved using both real-time fluorescence quantitative PCR (qPCR) and a custom-made droplet digital PCR (dPCR) system. Under optimized conditions, the assay using dPCR achieved a limit of detection of 0.015 ng/mL, a 6-fold improvement over qPCR. The validation of our method using major agricultural produce, such as corn, flour, and soy sauce, shows excellent recoveries ranging from 85.77 % to 108.28 %, with a total assay time of less than 2 h. Furthermore, we compared our results with a high-performance liquid chromatography (HPLC) method for AFB<sub>1</sub> content in corn and flour, revealing consistent outcomes (r = 0.9847). These results demonstrate the potential of nanobody-based PD-IPCR as a reliable tool for detecting mycotoxins.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"286 ","pages":"Article 117622"},"PeriodicalIF":10.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in molecularly imprinted polymers toward biomedical healthcare devices 分子印迹聚合物在生物医学保健设备上的最新进展

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-05-27 DOI: 10.1016/j.bios.2025.117637

Youngjun Seo , Yonghwan Kim , Jaeheung Kim , Hyo Gi Jung , Jae Won Jang , Hyunji Kim , Kyo Seon Hwang , Dongtak Lee , Sang Won Lee , Jeong Hoon Lee , Dae Sung Yoon

{"title":"Recent advances in molecularly imprinted polymers toward biomedical healthcare devices","authors":"Youngjun Seo , Yonghwan Kim , Jaeheung Kim , Hyo Gi Jung , Jae Won Jang , Hyunji Kim , Kyo Seon Hwang , Dongtak Lee , Sang Won Lee , Jeong Hoon Lee , Dae Sung Yoon","doi":"10.1016/j.bios.2025.117637","DOIUrl":"10.1016/j.bios.2025.117637","url":null,"abstract":"<div><div>Following the increase in the global aging population, the importance of monitoring various disease-related biomarkers at an early stage has increased to ensure the health of the elderly. In an aging society, point-of-care (POC) systems are needed to care for elderly people who have physical and mental disorders. In light of the increasing prominence of POC systems, there is a growing effort to address the limitations associated with conventional receptors in wearable devices by substituting them with molecularly imprinted polymer (MIP). MIPs are well-known synthetic receptors engineered with specific binding cavities based on the \"lock and key\" principle. They could be synthesized in various configurations depending on specific types of monomers and templates. Compared to conventional receptors such as antibodies and aptamers, MIPs exhibit outstanding advantages, including good stability, cost-effectiveness, ease of synthesis, reusability, and broad applicability. These advantages have led to their extensive application in biomedical healthcare devices. This review summarizes a comparative analysis of sensors utilized for the detection of disease-specific biomarkers through diverse MIP applications and methodologies. Furthermore, we evaluated recent advancements in the miniaturization of MIPs and their integration with wearable biomedical devices for comprehensive healthcare monitoring applications. Finally, we delineated the existing limitations that need to be surmounted for the effective implementation of MIP-based biomedical devices and outlined future objectives for the enhancement of wearable and wireless biomedical healthcare monitoring devices.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117637"},"PeriodicalIF":10.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced SERS sensor with horizontally aligned sub-5 nm silicon nanowires and high-density silver nanoparticles for ultra-sensitive molecular analysis 先进的SERS传感器，具有水平对齐的亚5纳米硅纳米线和高密度银纳米粒子，用于超敏感分子分析

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-05-26 DOI: 10.1016/j.bios.2025.117633

Peiyun Feng , Juyeon Seo , Jianlin Li , Hyun Young Jung , Yung Joon Jung

{"title":"Advanced SERS sensor with horizontally aligned sub-5 nm silicon nanowires and high-density silver nanoparticles for ultra-sensitive molecular analysis","authors":"Peiyun Feng , Juyeon Seo , Jianlin Li , Hyun Young Jung , Yung Joon Jung","doi":"10.1016/j.bios.2025.117633","DOIUrl":"10.1016/j.bios.2025.117633","url":null,"abstract":"<div><div>Surface-enhanced Raman scattering (SERS) is indispensable for its unparalleled ability to amplify Raman signals and detect low-concentration analytes. However, its full potential remains unrealized due to challenges in fabricating complex and tightly controlled nanostructured substrates with an optimized balance of synergistic chemical and electromagnetic enhancement. Here, we present an ultra-sensitive SERS sensor utilizing high-density small silver nanoparticles (AgNPs, 1–10 nm) coated onto highly dense, horizontally aligned sub-5 nm silicon nanowires (SiNWs) designed to achieve single-molecule detection. By a well-controlled dip-coating technique without reducing agents, AgNPs were nucleated and tightly adhered to sub-5 nm SiNWs synthesized using a catalyst-free chemical vapor etching (CVE) method. Such innovative structures significantly increase SERS sensitivity by minimizing interparticle gaps and ensuring more stable and consistent signal amplification across the substrate. Using Rhodamine 6G (R6G) and crystal violet (CV) as probe molecules, the sensor demonstrates exceptional dual-sensing capabilities, achieving precise detection of R6G at trace concentrations as low as 10<sup>−12</sup> M, indicative of single-molecule sensitivity. The sensor's performance was validated using Raman mapping, revealing stable and reproducible single-molecule detection with high-resolution spectra. Our SERS sensor system, based on aligned sub-5 nm SiNW arrays decorated with high-density AgNPs, enables ultra-sensitive molecular detection, providing significant advancements in environmental pollution monitoring and biomedical analysis applications.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"286 ","pages":"Article 117633"},"PeriodicalIF":10.7,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144178610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0