Biosensors and Bioelectronics最新文献_第2页

High-sensitivity PD-L1 immune checkpoint imaging in microtumors for immunotherapy using near-infrared persistent luminescence nanoparticles. 利用近红外持续发光纳米粒子在微肿瘤免疫治疗中的高灵敏度PD-L1免疫检查点成像。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-10-06 DOI: 10.1016/j.bios.2025.118070

Ruoping Wang, Junpeng Shi, Chen Wu, Xia Sun, Xiaofang Luo, Yile Kang, Yun Zhang

{"title":"High-sensitivity PD-L1 immune checkpoint imaging in microtumors for immunotherapy using near-infrared persistent luminescence nanoparticles.","authors":"Ruoping Wang, Junpeng Shi, Chen Wu, Xia Sun, Xiaofang Luo, Yile Kang, Yun Zhang","doi":"10.1016/j.bios.2025.118070","DOIUrl":"https://doi.org/10.1016/j.bios.2025.118070","url":null,"abstract":"The efficacy of programmed cell death-ligand 1 (PD-L1)-based immune checkpoint blockade therapy (ICBT) depends on the tumor PD-L1 expression level. Accurate evaluation of tumor PD-L1 is essential to identify patients who will benefit most effectively and to develop appropriate treatment strategies. However, high-sensitivity evaluation of microtumor PD-L1 expression remains challenging in current ICBT landscape. Here, we develop a PD-L1-targeted near-infrared (NIR) persistent luminescence (PersL) nanoprobe, termed mZ-PD-L1, and establish a high-sensitivity PD-L1 imaging technique based on NIR PersL. This method allows high-sensitivity and precise imaging of PD-L1 in microtumors and micrometastases in vivo. mZ-PD-L1 exhibits biowindow-activated reproducible NIR PersL properties, along with exceptional PD-L1 targeting capability. By conducting in vivo NIR PersL imaging, mZ-PD-L1 facilitates high-sensitivity visualization of tumor PD-L1 expression. Using mZ-PD-L1, we achieve high-sensitivity and precise imaging of PD-L1 in microtumors (≤1 mm) and lymph node micrometastases, with imaging signal-to-background ratios as high as 27 and 37, respectively. This study provides a strategy to precisely and comprehensively evaluate PD-L1 in microtumors or micrometastases, significantly broadening the ICBT applications and offering a new method for personalized and precise ICBT.","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"292 ","pages":"118070"},"PeriodicalIF":10.5,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145243443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasmonic-photonic metasensor with mesoporous Nb2C MXene for the detection of IgAN extracellular vesicles 介孔Nb2C MXene等离子体-光子传感器检测IgAN细胞外囊泡

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-10-04 DOI: 10.1016/j.bios.2025.118068

Yameng Li, Peilin Wang, Wenyan Li, Zhenrun Li, Fangyan Ji, Qiang Ma

{"title":"Plasmonic-photonic metasensor with mesoporous Nb2C MXene for the detection of IgAN extracellular vesicles","authors":"Yameng Li, Peilin Wang, Wenyan Li, Zhenrun Li, Fangyan Ji, Qiang Ma","doi":"10.1016/j.bios.2025.118068","DOIUrl":"10.1016/j.bios.2025.118068","url":null,"abstract":"<div><div>Two-dimensional metasurface is composed of functional nanounits at the subwavelength scale, which can manipulate the propagation and characters of light. However, metasurfaces are still in the stage of theoretical study, which lack practical application exploration. Herein, we designed a novel metasurface-based biosensor as metasensor for the detection of extracellular vesicles. Firstly, the mesoporous Nb2C MXene (mesoNb2C) nanosheets had in-plane structure with the uniform pore size, which improved both electrochemical and luminescence performance. Furthermore, a novel metasurface was prepared consisting of plasmonic Au nanorings (Au NRs) layer and ZnS@SiO2 photonic crystal as sensing interface. The constructed plasmonic-photonic metasensor can adjust the reflection wavelength. So, the electrochemiluminescence (ECL) signal of mesoNb2C can be reflected by the ZnS@SiO2 photonic crystal and significantly enhanced by the Au NRs array with the local surface plasmon resonance hotspots in the metasensor. Finally, the metasensor was used to evaluate the expression of miRNA-23b-3p in the urinary EVs of IgA nephropathy patients. The metasensor showed a good linear relationship between the ECL intensity and the logarithm of miRNA-23b-3p concentration from 1 fM to 100 nM, and the limit of detection was 0.45 fM, which can be used to effectively distinguish between normal people, mild patients and severe patients.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"292 ","pages":"Article 118068"},"PeriodicalIF":10.5,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AI-enhanced diagnosis of atrial arrhythmia using 3D-printed origami ECG sensors. 应用3d打印折纸心电传感器人工智能增强心房心律失常诊断。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-10-04 DOI: 10.1016/j.bios.2025.118069

Yiting Chen, Jake Non, Zakhar Vozovik, Woo Soo Kim

{"title":"AI-enhanced diagnosis of atrial arrhythmia using 3D-printed origami ECG sensors.","authors":"Yiting Chen, Jake Non, Zakhar Vozovik, Woo Soo Kim","doi":"10.1016/j.bios.2025.118069","DOIUrl":"https://doi.org/10.1016/j.bios.2025.118069","url":null,"abstract":"Traditional Electrocardiogram (ECG) sensors using silver/silver chloride (Ag/AgCl) electrodes suffer from skin irritation, short shelf life, single-use limitation, and environmental waste. Here, we introduce a sustainable 3D-printed origami-structured ECG sensor featuring dry attachment, accurate measurement, reusability, and AI-powered diagnosis. The origami design combines mechanical stretchability, robustness, and self-adhesive, while the patterned carbon-based conductive ink provides high electrical conductivity (5681 ± 122.5 S/m), flexibility (bending to 2.5 mm radius), and biocompatibility, altogether offering a sustainable alternative to Ag/AgCl electrodes. The resulting sensor delivers accurate ECG signals comparable to commercial Ag/AgCl electrodes, in addition to an AI-enabled swift classification system that combines continuous wavelet transform (CWT) and a customized convolutional neural network (CNN) for real-time pre-diagnosis of one sinus rhythm and ten arrhythmias types from ECG scalogram images. This system monitors continuously for up to 34 h, promoting early detection of transient cardiac conditions and personalized health monitoring. This advancement establishes a new standard for AI-enhanced, eco-friendly ECG sensors, with significant potential for applications in remote healthcare, emergency diagnostics, and real-time cardiac monitoring.","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"292 ","pages":"118069"},"PeriodicalIF":10.5,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical VWF Biosensors Based on Two-Step Synthesized rGO@AuNPs Nanocomposites for Early Prediction of ECMO Bleeding Complications. 基于两步合成rGO@AuNPs纳米复合材料的电化学VWF生物传感器早期预测ECMO出血并发症。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-10-03 DOI: 10.1016/j.bios.2025.118064

Zihan Kou, Jiameng Li, Linlin Li, Qianlong Xue, Yuansen Chen, Yikuan Liu, Xiangyan Meng, Haojun Fan

{"title":"Electrochemical VWF Biosensors Based on Two-Step Synthesized rGO@AuNPs Nanocomposites for Early Prediction of ECMO Bleeding Complications.","authors":"Zihan Kou, Jiameng Li, Linlin Li, Qianlong Xue, Yuansen Chen, Yikuan Liu, Xiangyan Meng, Haojun Fan","doi":"10.1016/j.bios.2025.118064","DOIUrl":"https://doi.org/10.1016/j.bios.2025.118064","url":null,"abstract":"Extracorporeal membrane oxygenation (ECMO) is a life-saving technology for patients with severe cardiopulmonary failure. However, bleeding complications remain a major clinical challenge, largely due to high shear stress-induced Von Willebrand factor (VWF) dysfunction. Existing methods often lack timeliness and sensitivity for VWF detection in early bleeding risk assessment. Here, we proposed an electrochemical biosensor that combined Two-Step Synthesized graphene oxide-gold nanoparticles (rGO@AuNPs) nanocomposites modified screen-printed electrodes and a miniaturized wireless communication circuit for point-of-care testing (POCT) of VWF levels. The introduction of rGO@AuNPs significantly enhanced the sensing performance, achieving a low limit of detection of 0.39 pg/mL for VWF within 15 min. Moreover, there are Pearson correlation coefficients of 0.926 and 0.974 between the VWF biosensor and ELISA for porcine models and clinical samples. Notably, VWF degradation was associated with high shear stress, and VWF depletion was observed at the same time of APTT prolongation, suggesting its utility as a biomarker for bleeding risk. Its rapid, portable, and cost-effective design supports POCT, offering a promising tool for early monitoring and intervention in ECMO-related bleeding.","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"292 ","pages":"118064"},"PeriodicalIF":10.5,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valve-controlled needle-type "mix and detect" integrated distance-based sensor with visual color zoning for point-of-care testing of tacrolimus. 带视觉颜色分区的阀控针式“混合检测”集成距离传感器用于他克莫司的即时检测。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-10-03 DOI: 10.1016/j.bios.2025.118065

Lulu Wu, Fei Yang, Yuchan Ma, Li Tang, Hongmei Liu, Wenwen Wang, Hui Ouyang, Zhifeng Fu, Yong He

{"title":"Valve-controlled needle-type \"mix and detect\" integrated distance-based sensor with visual color zoning for point-of-care testing of tacrolimus.","authors":"Lulu Wu, Fei Yang, Yuchan Ma, Li Tang, Hongmei Liu, Wenwen Wang, Hui Ouyang, Zhifeng Fu, Yong He","doi":"10.1016/j.bios.2025.118065","DOIUrl":"https://doi.org/10.1016/j.bios.2025.118065","url":null,"abstract":"The concentration of tacrolimus (TAC) must be controlled within a certain range for homebound patients on long-term TAC after organ transplantation to avoid acute rejection risk. Routine clinical TAC detections suffer from expensive instruments and the lag time between sampling and results, making it difficult to apply in resource-limited environment. Rapid and user-friendly point-of-care testing biosensors significantly benefit homebound transplantation patients by avoiding the need for frequent medical visits and reducing meaningless healthcare expenditures. In this study, a simple and portable visual valve-controlled needle-type \"mix and detect\" integrated distance-based biosensor was carefully designed for homebound patients. Pt/MnO2 NRs nanocomposite with enhanced catalytic activity and stability was used as the signal probe to realize highly sensitive detection. The lateral flow immunoassay-based detection process can be completed within 13 min. The dynamic detection range of TAC was 0.10-1000 ng mL-1 with a detection limit of 0.03 ng mL-1, which covered the whole therapeutic drug concentration range. The recoveries of spiked samples in pre-diluted milk, urine, saliva, and plasma ranged from 88.26 % to 116.72 %, confirming the reliability and applicability of this biosensor in real samples. There was no significant difference between the results of the developed method and the clinical reference method, indicating its reliability for further potential application. Concentration-dependent visual color zones facilitated homebound transplantation patients on long-term TAC to make reasonable medical choices. It showed unparalleled remarkable performance in real-time detection, offering a new reference method for future medical field diagnosis when the large, multicenter cohort studies were conducted.","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"292 ","pages":"118065"},"PeriodicalIF":10.5,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145243434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel electrochemiluminescence resonance energy transfer biosensor driven by CRISPR-Cas12a system for ctDNA detection. 基于CRISPR-Cas12a系统驱动的新型电化学发光共振能量转移生物传感器用于ctDNA检测。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-10-03 DOI: 10.1016/j.bios.2025.118067

Xiaocui Huang, Alian Wang, Zhenyu Lin, Yunpeng Xu, Jianping Zheng

引用次数: 0

DNA centipede nanowires drive rapid picomolar microRNA detection in cells and serum. DNA蜈蚣纳米线驱动细胞和血清中快速小摩尔microRNA检测。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-10-03 DOI: 10.1016/j.bios.2025.118066

Zhixiong Dong, Yangyang Liu, Zhiwei Zhu, Xianghan Song, Huo Xu, Wei Cui, Fanggui Shao

{"title":"DNA centipede nanowires drive rapid picomolar microRNA detection in cells and serum.","authors":"Zhixiong Dong, Yangyang Liu, Zhiwei Zhu, Xianghan Song, Huo Xu, Wei Cui, Fanggui Shao","doi":"10.1016/j.bios.2025.118066","DOIUrl":"https://doi.org/10.1016/j.bios.2025.118066","url":null,"abstract":"Accurate and rapid detection of microRNA (miRNA) is essential for clinical diagnostics and molecular biology. Here, we report a DNA centipede nanoprobe (DCNP)-based localized catalytic hairpin assembly (LCHA) strategy for ultrasensitive detection of miR-21 in cells and serum. In this system, DNA nanowires spatially confine two reactive hairpins (HP1 and HP2) to enhance local concentration and accelerate hybridization kinetics, leading to a fivefold sensitivity improvement over free-CHA. The DCNP enables rapid detection within 40 min, with a limit of detection down to 100 pM, and offers robust performance in complex biological fluids without the need for transfection reagents. High specificity is achieved against homologous and mutant miRNAs. Intracellular imaging of miR-21 in MCF-7 cells displays strong fluorescence signals, consistent with qRT-PCR. Clinical serum samples from colon cancer patients and healthy donors are clearly distinguished, yielding a receiver operating characteristic curve with an AUC of 1.00. This LCHA system demonstrates high sensitivity, rapid response, and reliable intracellular delivery, making it a promising candidate for early cancer diagnostics and point-of-care applications.","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"292 ","pages":"118066"},"PeriodicalIF":10.5,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amplification of electrochemical signal by moving interdigitated electrode array for highly sensitive detection of Alzheimer's disease markers in blood. 移动交叉指状电极阵列放大电化学信号，用于血液中阿尔茨海默病标志物的高灵敏度检测。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-10-03 DOI: 10.1016/j.bios.2025.118063

Bappa Sarkar, Md Tareq Rahman, M Mahabubur Rahman, Sarwar Hossen, Li Zhenguo, Nabil H Bhuiyan, Joon S Shim

{"title":"Amplification of electrochemical signal by moving interdigitated electrode array for highly sensitive detection of Alzheimer's disease markers in blood.","authors":"Bappa Sarkar, Md Tareq Rahman, M Mahabubur Rahman, Sarwar Hossen, Li Zhenguo, Nabil H Bhuiyan, Joon S Shim","doi":"10.1016/j.bios.2025.118063","DOIUrl":"https://doi.org/10.1016/j.bios.2025.118063","url":null,"abstract":"Electrochemical biosensors are widely utilized in disease diagnosis due to their high sensitivity, low detection limits, and cost-effectiveness. However, conventional electrochemical ELISA (e-ELISA) measurements face several challenges and limitations, including limited analyte volume, reduced sensitivity, signal instability, and surface fouling. To overcome the limitations, we developed a novel vertically moving sensor system integrated with a laser-induced graphene metal interdigitated array (LIG-MIDA) biosensor modified with carbon nanotubes and silver nanoparticles. The amplitude (4 mm) and speed (8 mm/s) of the sensor movement, as well as the electrode nanoparticle composition (2 % MWCNTs and 10 % AgNPs), were optimized to improve signal amplification and reproducibility. The proposed moving sensor was first demonstrated by measuring p-aminophenol (PAP), which exhibited higher signal stability, lower standard deviations, and a wide linear range (1 mM-10 pM) than the conventional static sensor. The proposed moving sensor achieved detection limits of 0.63 pg/mL for Aβ-40 and 0.78 pg/mL for Aβ-42 in buffer, approximately four times lower than that of the static sensors. Moreover, the proposed sensor was further verified by measuring the Aβ-42 biomarker in real plasma, which showed a similar sensitivity to buffer samples. Therefore, the developed system is clinically applicable for detecting Alzheimer's disease in its early stage.","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"292 ","pages":"118063"},"PeriodicalIF":10.5,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145243406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical biosensor for early Alzheimer's detection and patient risk stratification using plasma exosomes. 电化学生物传感器用于早期阿尔茨海默病检测和血浆外泌体患者风险分层。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-10-01 DOI: 10.1016/j.bios.2025.118061

Rosanna Rossi, Amanda Cano, Arnau Pallarès-Rusiñol, Agustín Ruiz, Merce Martí, Maria Isabel Pividori

{"title":"Electrochemical biosensor for early Alzheimer's detection and patient risk stratification using plasma exosomes.","authors":"Rosanna Rossi, Amanda Cano, Arnau Pallarès-Rusiñol, Agustín Ruiz, Merce Martí, Maria Isabel Pividori","doi":"10.1016/j.bios.2025.118061","DOIUrl":"https://doi.org/10.1016/j.bios.2025.118061","url":null,"abstract":"Alzheimer's Disease (AD) is the leading cause of dementia, accounting for 60-70 % of cases worldwide. Early diagnosis remains challenging due to the limitations of current diagnostic tools, which are costly, invasive, and suffer from low patient compliance. Blood-based biomarkers, particularly plasma brain-derived exosomes (BDEs), have emerged as a promising alternative since they carry AD-related molecules and can be isolated non-invasively. In this study, an immunoassay was developed to isolate BDEs using magnetic particles functionalized with an anti-neuroligin-3 (NLGN3) antibody, while the AD-related marker β-secretase (BACE-1) was detected on the captured exosomes. This is the first report combining NLGN3 for for the isolation of BDEs with BACE-1 as a detection target, establishing a novel biomarker panel for AD diagnostics. The assay was evaluated across three readout platforms-optical, chemiluminescent, and electrochemical-with detection limits in the range of 104-105 exosomes μL-1. Among them, the portable electrochemical platform achieved the improved LOD (1.51 × 104 exosomes μL-1, R2 = 0.9829). Plasma samples from patients with AD, mild cognitive impairment (MCI), and healthy controls were analyzed, revealing differences in exosomal BACE-1 levels (p < 0.1, t-test). These findings demonstrate, for the first time, an in vitro diagnostic approach based on a portable electrochemical biosensor for early AD detection in plasma through a novel exosomal biomarker panel. Compared to conventional diagnostics, this biosensor offers a non-invasive and cost-effective solution for AD screening, with the potential to support earlier intervention and patient risk stratification.","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"292 ","pages":"118061"},"PeriodicalIF":10.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemiluminescent biosensors for pharmaceutical analysis: innovations, challenges, and future prospects. 用于药物分析的化学发光生物传感器：创新、挑战和未来展望。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-10-01 DOI: 10.1016/j.bios.2025.118059

Muhammad Saqib, Yun Lei, Guobao Xu, Raheel Akram, Ai-Lin Liu

{"title":"Chemiluminescent biosensors for pharmaceutical analysis: innovations, challenges, and future prospects.","authors":"Muhammad Saqib, Yun Lei, Guobao Xu, Raheel Akram, Ai-Lin Liu","doi":"10.1016/j.bios.2025.118059","DOIUrl":"https://doi.org/10.1016/j.bios.2025.118059","url":null,"abstract":"Chemiluminescent (CL) biosensors have rapidly emerged as indispensable analytical tools within the multifaceted domains of pharmaceutical research, development, and clinical diagnostics. This prominence stems from their intrinsic attributes such as higher sensitivity, low background noise, and inherent amenability to miniaturization for portable, real-time readout capabilities. This comprehensive review meticulously presents and critically evaluates the most recent innovations in CL-based biosensing platforms specifically engineered for diverse pharmaceutical applications. A primary focus is directed towards the pioneering innovations in the development of CL biosensors with enhanced and tailored emission profiles, the strategic and synergistic integration with an array of sophisticated signal amplification strategies, and the ingenious adaptation of CL detection techniques into highly portable, integrated, and microfluidic devices. Key applications, encompassing stringent drug quality control and authentication, precise pharmacokinetic analysis for personalized medicine, and the critical, rapid detection of counterfeit and substandard medications, are discussed with a rigorous, critical lens, highlighting both their transformative potential and current practical limitations. Furthermore, the review critically assesses emerging prospects, including the advent of advanced wearable sensors for continuous monitoring, the transformative potential of artificial intelligence-assisted diagnostics for data interpretation and predictive modeling, and the imperative for sustainable, eco-friendly reagent design. This article thus serves as both a comprehensive technical resource, elucidating the intricate scientific underpinnings and practical advancements, and a forward-looking perspective on the pivotal and evolving role of chemiluminescent biosensing in modern pharmaceutical analysis.","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"292 ","pages":"118059"},"PeriodicalIF":10.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145243445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0