Biosensors and Bioelectronics最新文献

{"title":"A DNA tetrahedron-based light-controlled intramolecular CHA for spatiotemporal imaging of miRNA in living cells with high sensitivity and imaging contrast","authors":"Jing Peng ,&nbsp;Simin Liu ,&nbsp;Ziyue Qiu ,&nbsp;Luyin Zhang ,&nbsp;Ziyan Dong ,&nbsp;Qianying Zhou ,&nbsp;Ting Huang ,&nbsp;Yanfei Zhang ,&nbsp;Jingyu Shi ,&nbsp;Jin-Xiang Chen ,&nbsp;Zong Dai ,&nbsp;Jun Chen","doi":"10.1016/j.bios.2025.117565","DOIUrl":"10.1016/j.bios.2025.117565","url":null,"abstract":"<div><div>miRNA is an effective disease diagnostic biomarker. Developing efficient miRNA in living cells analysis methods, holds significant importance for disease diagnosis and understanding disease progression. Strategies combining DNA nanostructures and enzyme-free amplification have been developed for highly sensitive and rapid imaging of miRNA. However, due to nonspecific amplification of enzyme-free amplification inherent in the probe assembly process and during transfection, these strategies suffer from significant background signals and reduced sensitivity, limiting their further application. To overcome these limitations, we have developed a DNA tetrahedral-based and photocontrolled intramolecular CHA strategy (TCHA-P) to achieve spatiotemporal, highly sensitive, and accurate imaging of miRNA in living cells. In this strategy, the DNA tetrahedron not only serves as a transfection vector but also enhances the local concentration of reactant probes, boosting the reaction kinetics and sensitivity of CHA. Additionally, the light-controlled group (PC-linker) can trigger CHA at selected times and locations, enhancing the spatiotemporal precision of miRNA imaging and ensuring high sensitivity and accuracy by eliminating nonspecific amplification during the assembly and transfection processes. Using miR-10b as a proof-of-concept model, the proposed strategy demonstrated higher signal-to-noise ratio compared to traditional CHA, with a detection limit of 28 pM. Living cell imaging experiments showed that the strategy could effectively differentiate the expression of miR-10b between normal breast cells and breast cancer cells, providing high imaging contrast. The strategy holds promise for broadening the path of nucleic acid amplification-based imaging and is expected to become an effective tool in nucleic acid-based disease diagnosis, treatment, and drug development.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"284 ","pages":"Article 117565"},"PeriodicalIF":10.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143931787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sulfatase-mediated peroxidase-like activity: A chemiluminescence-based platform for high-throughput screening of natural inhibitors in cancer therapy","authors":"Mengyuan Cui ,&nbsp;Wenqing Li ,&nbsp;Li Liu ,&nbsp;Yan Chen ,&nbsp;Huijia Liu ,&nbsp;Min Ji ,&nbsp;Fang Yang ,&nbsp;Peng Wang","doi":"10.1016/j.bios.2025.117562","DOIUrl":"10.1016/j.bios.2025.117562","url":null,"abstract":"<div><div>Sulfatase, traditionally known for its role in sulfate ester hydrolysis, has recently emerged as a potential player in tumor biology through its involvement in oxidative stress pathways. Here, we demonstrate for the first time that sulfatase exhibits peroxidase-like activity, catalyzing the generation of singlet oxygen (¹O₂) in the presence of oxygen. Based on the sulfatase-dependent ¹O₂ generation, the developed adamantly-enolether chemiluminescence probe QM-CF for imaging tumors of high sulfatase expression further verified the theory that sulfatase can be involved in tumor development. High-throughput screening (HTS) of natural compounds and clinical drugs identified scutellarin and sinomenine as potent sulfatase inhibitors that suppress tumor growth in mice. Mechanistic investigations revealed that these inhibitors modulate oxidative stress by downregulating MAPK and NF-κB pathways. Our findings unveil a previously unappreciated role of sulfatase in tumor-related oxidative stress and provide a promising platform for the discovery of novel sulfatase inhibitors, and advancing cancer therapeutics.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"284 ","pages":"Article 117562"},"PeriodicalIF":10.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning classification of quorum sensing-induced bacterial aggregation using flow rate assays on paper chips toward bacterial species identification in potable water sources","authors":"Seung-Ju Choi ,&nbsp;Min Hee Lee ,&nbsp;Yan Liang ,&nbsp;Ethan C. Lin ,&nbsp;Bradley Khanthaphixay ,&nbsp;Preston J. Leigh ,&nbsp;Dong Soo Hwang ,&nbsp;Jeong-Yeol Yoon","doi":"10.1016/j.bios.2025.117563","DOIUrl":"10.1016/j.bios.2025.117563","url":null,"abstract":"<div><div>Preventing waterborne disease caused by bacteria is especially important in low-resource settings, where skilled personnel and laboratory equipment are scarce. This work reports a straightforward method for classifying bacterial species by monitoring the capillary flow rates on a multi-channel paper microfluidic chip, where quorum sensing (QS)-induced bacterial aggregation leads to measurable changes in flow rates, enabling species differentiation. It required no fluorescent molecules, microscope, particles, covalent conjugation, or surface immobilization. Five representative QS molecules and control were added to each bacterial sample, and their different extents of bacterial aggregation resulted in varied flow rates. Flow rates were collected for the duration of the flow to build the learning database, and the XGBoost machine learning algorithm predicted the accuracy for classifying ten bacterial species, including 7 gram-negative and 3 gram-positive species. Three different algorithms were developed for high, medium, and low bacterial concentration ranges, and the classification accuracies of all the algorithms exceeded 75.0 %. Using XGBoost and the previously established database, we tested bacteria in the field water samples and successfully predicted the dominant species. The technology developed in this study, using only QS molecules and a paper microfluidic chip, offers a simple system for detecting microorganisms in drinking water to help prevent waterborne diseases.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"284 ","pages":"Article 117563"},"PeriodicalIF":10.7,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A screen-printed microelectrode for detection of hydrogen peroxide in solid tumor in vivo","authors":"Luhan Lin ,&nbsp;Hongli Zhao ,&nbsp;Jian Hou ,&nbsp;Minbo Lan","doi":"10.1016/j.bios.2025.117561","DOIUrl":"10.1016/j.bios.2025.117561","url":null,"abstract":"<div><div>Hydrogen peroxide (H₂O₂), a crucial redox signaling molecule and neuromodulator, is closely associated with pathological processes, including cancer progression and neurodegenerative disorders. Current methods for in vivo H₂O₂ detection, such as fluorescence imaging and chemiluminescence, suffer from the limitation of spatial resolution and invasiveness, which makes it difficult to monitor oxidative stress gradients in deep-seated tumors. Therefore, this research developed an implantable triple-electrode biosensor fabricated via screen-printing technology based on carboxylated multi-walled carbon nanotubes (MWCNT) and Prussian blue (PB) nanocomposites. The biosensor presented dual linear detection ranges of 0.8–1126 μM (R² = 0.9937) and 1286–3766 μM (R² = 0.9939) with a 0.47 μM detection limit. It demonstrated a &gt;95 % specificity compared with other interfering substances and maintained 93.2 % signal retention over 30 days. Particularly, in situ implantation in melanoma-bearing mice with one-week-growth-time solid tumors revealed the H₂O₂ levels 12- to 18-fold higher than in normal tissues, consistent with cancer-associated oxidative stress mechanisms. This platform addresses challenges such as rapid enzymatic degradation and microenvironmental complexity, enabling invasive profiling of H₂O₂ detection in solid tumors.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"284 ","pages":"Article 117561"},"PeriodicalIF":10.7,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticle-supported, rapid, digital quantification of neutralizing antibodies against SARS-CoV-2 variants","authors":"Seyedsina Mirjalili ,&nbsp;Md Ashif Ikbal ,&nbsp;Ching-Wen Hou ,&nbsp;Maziyar Kalateh Mohammadi ,&nbsp;Yeji Choi ,&nbsp;Laimonas Kelbauskas ,&nbsp;Laura A. VanBlargan ,&nbsp;Brenda G. Hogue ,&nbsp;Vel Murugan ,&nbsp;Michael S. Diamond ,&nbsp;Chao Wang","doi":"10.1016/j.bios.2025.117549","DOIUrl":"10.1016/j.bios.2025.117549","url":null,"abstract":"<div><div>The measurement of neutralizing immune responses to viral infection is essential, given the heterogeneity of human immunity and the emergence of new virus strains. However, neutralizing antibody (nAb) assays often require high-level biosafety containment, sophisticated instrumentation, and long detection times. Here, as a proof-of-principle, we designed a nanoparticle-supported, rapid, electronic detection (NasRED) assay to assess the neutralizing potency of monoclonal antibodies (mAbs) against SARS-CoV-2. The gold nanoparticles (AuNPs) coated with human angiotensin-converting enzyme 2 (ACE2) protein as nAb potency reporters were mixed with the mAbs to be tested, as well as streptavidin-conjugated multivalent spike (S) protein or their receptor binding domains (RBD). High-affinity and ACE2-competitive nAbs alter the S (or RBD)-to-ACE2 binding level and modulate AuNP cluster formation and precipitation. The amount of free-floating AuNP reporters is quantified by a semiconductor-based readout system that measures the AuNPs' optical extinction, producing nAb signals that can differentiate SARS-CoV-2 variants (Wuhan-Hu-1, Gamma, and Omicron). The modular design nature, short assay time (less than 30 min), and portable and inexpensive readout system make this NasRED-nAb assay applicable to measuring vaccine potency, immune responses to infection, and the efficacy of antibody-based therapies.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"285 ","pages":"Article 117549"},"PeriodicalIF":10.7,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Mito-targeted, pH-sensitive type I photosensitizer for the diagnosis and therapy in bone metastasis of triple negative breast cancer by activating pyroptosis pathway","authors":"Ya-Xi Ye ,&nbsp;Jian-Cheng Pan ,&nbsp;Yu Jiang ,&nbsp;Pei Tang ,&nbsp;Hai-Chao Wang ,&nbsp;Xin-Ai Shi ,&nbsp;Xing-Tao Zhang ,&nbsp;Hai-Liang Zhu ,&nbsp;Xin-Hua Liu","doi":"10.1016/j.bios.2025.117559","DOIUrl":"10.1016/j.bios.2025.117559","url":null,"abstract":"<div><div>Rational design of multifunctional molecules integrating diagnostic and therapeutic capabilities represents a cutting-edge yet challenging frontier in modern medicine. In this study, we present the first report on <strong>Rh-HB</strong>, a smart near-infrared (NIR) molecular probe engineered for mitochondria-targeted theranostics in triple-negative breast cancer (TNBC) bone metastasis. As a ratiometric, dual-excitation pH-sensitive fluorescent probe, <strong>Rh-HB</strong> enables real-time monitoring of mitochondrial pH fluctuations associated with early-stage metastatic progression. Moreover, <strong>Rh-HB</strong> functions as a highly efficient type I photosensitizer, generating cytotoxic reactive oxygen species (ROS) such as superoxide anion (O₂^•-) and hydroxyl radicals (·OH) upon light irradiation within mitochondria. This localized ROS production overcomes the limitations of short diffusion distances, ensuring effective oxidative damage. Crucially, <strong>Rh-HB</strong> induces severe mitochondrial oxidative stress, triggering pyroptosis in TNBC bone metastasis models. Thus, <strong>Rh-HB</strong> serves as a proof-of-concept theranostic agent, offering a promising strategy for combating bone metastasis of TNBC.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"283 ","pages":"Article 117559"},"PeriodicalIF":10.7,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Singlicate bead-based immunoassay for biomarker quantitation in sub-microliter volume sample","authors":"Sang Hyun Lee ,&nbsp;Myeongsik Shin ,&nbsp;Chang Heon Lee,&nbsp;Taek Dong Chung","doi":"10.1016/j.bios.2025.117537","DOIUrl":"10.1016/j.bios.2025.117537","url":null,"abstract":"<div><div>Point-of-care testing (PoCT) devices are becoming increasingly essential for widespread disease diagnostics. Hence, it becomes necessary to develop solutions for detecting biomarkers from minimal-volume samples while remaining accessible and user-friendly. This study presents a new method called FoCUS (Forward scattering Correction Using a Single-bead). FoCUS combines tiny beads floating in liquid with a small chip to measure very small amounts of sample. It can analyze a single drop using just one bead. The platform employs a novel signal correction strategy to enhance fluorescence signal sensitivity using forward scattering signals obtained simultaneously. Compared to conventional methods, the FoCUS platform achieves a 1000-fold reduction in the required sample volume, a 2.7-fold decrease in the relative standard deviation of sample signals, and a 4.3-fold improvement in detection limits. The platform successfully detected relevant myoglobin levels from 0.1 μL of sample, demonstrating its potential for multiplex biomarker immunoassays in point-of-care applications where sample volumes are limited.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"284 ","pages":"Article 117537"},"PeriodicalIF":10.7,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aptamer-functionalized biomimetic supramolecular nanozyme constructed by dipeptide, glutaraldehyde and hemin and its excellent sensing performances for tetrodotoxin","authors":"Hongchao Ma ,&nbsp;Xifeng Ma ,&nbsp;Tiantian Dong ,&nbsp;Xinyu Bian ,&nbsp;Xiaokang Zhang ,&nbsp;Yanhui Wei","doi":"10.1016/j.bios.2025.117524","DOIUrl":"10.1016/j.bios.2025.117524","url":null,"abstract":"<div><div>Bioinspired nanozymes hold promise for simulating natural processes and creating optimized functional systems, but their application is hindered by limited catalytic activity and selectivity. These challenges can be addressed by reconstructing enzymatic active sites to enhance catalytic efficiency and integrating biological recognition units for specificity. In this work, we developed a peroxidase-mimicking nanozyme by stabilizing hemin on a supramolecular scaffold of diphenylalanine (FF) and glutaraldehyde (GA). To enable specific recognition, we conjugated a tetrodotoxin (TTX) aptamer, yielding the He@FF/GA-Apt composite nanozyme. This nanozyme demonstrated robust catalytic activity in 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation. The TTX aptamer conferred specific TTX recognition, with the aptamer-TTX complex blocking the nanozyme active site and reducing its activity. Based on this mechanism, we created a dual-mode TTX detection method using UV–vis spectroscopy and smartphone RGB analysis. The UV–vis mode achieved a linear range of 1.0–40.0 ng mL⁻¹ and a limit of detection (LOD) of 0.61 ng mL⁻¹, while the smartphone mode had a LOD of 1.43 ng mL⁻¹ in a linear range of 2.0–40.0 ng mL⁻¹. Both methods performed well in real samples, with recoveries of 96.29 %–102.57 % (UV–vis mode) and 92.07 %–109.46 % (RGB mode). In comparation, the UV–vis mode offers high sensitivity but requires lab equipment, whereas smartphone RGB mode enables rapid on-site detection despite a little lower sensitivity. This work provides a promising approach for developing target-specific nanozyme sensors.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"283 ","pages":"Article 117524"},"PeriodicalIF":10.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-operando electrochemical detection of hydrogen peroxide during bacteria damage using ZIF-67 derived electrocatalysts","authors":"María José Martín Martínez ,&nbsp;Alexander Suárez-Barajas ,&nbsp;Carlos M. Ramos-Castillo ,&nbsp;Lorena Álvarez Contreras ,&nbsp;Beatriz Liliana España Sánchez ,&nbsp;Noé Arjona","doi":"10.1016/j.bios.2025.117556","DOIUrl":"10.1016/j.bios.2025.117556","url":null,"abstract":"<div><div>In this study, ZIF-67 derived materials were obtained calcinating the ZIF-67 from 500 °C to 700 °C, and they were employed for the <em>in-operando</em> electrochemical detection of hydrogen peroxide formed during the bacterial damage of <em>E. coli</em> induced by the ZIF-67 as antibacterial agent. Transmission electron microscopy (TEM) micrographs revealed a shape transition during the calcination of ZIF-67 from a Co@C core@shell structure (500 °C) to a hollow carbon structure (600 °C). At 700 °C, the hollow structure unfolded to a laminar structure, forming a Co-N-C structure with Co-aggregated particles. The activity for H₂O₂ detection was shape-sensitive, finding that Co-N-C at 700 °C performed better by displaying a lower detection limit (LOD) of 1.64 ppm (48 μM) and a limit of quantification (LOQ) of 5.47 ppm (in phosphate buffer). The <em>in-operando</em> electrochemical tests confirmed that bacterial death can be followed by the detection of H₂O₂, finding a significant antibacterial activity until 1.5 h, after which the current dropped. These results were corroborated using the spread-plate method. Further tests with <em>S. Aureus</em> as a Gram-positive bacterium indicated that the proposed method can be extended to other bacteria.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"284 ","pages":"Article 117556"},"PeriodicalIF":10.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143931902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Target responsive-regulated CRISPR/Cas12a electrochemiluminescence sensing of salmonella typhimurium integrating ultrafine Pt NCs-anchored MXenes-boosted luminol/O2 system","authors":"Xiaobo Zhang ,&nbsp;Xiuwen Wang ,&nbsp;Longjiao Zhu ,&nbsp;Jinyan Zhu ,&nbsp;Qiuyue Zheng ,&nbsp;Jingli Yuan ,&nbsp;Wentao Xu ,&nbsp;Jijuan Cao","doi":"10.1016/j.bios.2025.117558","DOIUrl":"10.1016/j.bios.2025.117558","url":null,"abstract":"<div><div><em>Salmonella typhimurium</em> (<em>S. typhimurium</em>), as common and highly pathogenic foodborne pathogen, poses a significant risk to public safety worldwide. The development of highly sensitive, rapid and on-site method for <em>S. typhimurium</em> analysis is urgently needed to prevent bacterial infections. Herein, we introduced a CRISPR/Cas12a-driven electrochemiluminescence (ECL) sensor based on luminol/O₂ binary systems for <em>S. typhimurium</em> detection, employing ultrafine Pt nanoclusters-anchored 2D delaminated-MXenes (Pt NCs/D-MXenes) as the co-reactant accelerator. The ultrathin D-MXenes support regulates the size and dispersibility of Pt NCs and facilitates the full exposure of active sites, and synergistic interactions between D-MXenes and Pt NCs improves electrocatalytic properties toward the reduction of O₂, which promotes the generation of ROS for boosting ECL emission. Using target responsive-regulated CRISPR/Cas12a system, the ECL sensor for <em>S. typhimurium</em> showed a broad concentration range from 10¹ to 10⁶ CFU/mL and limit of detection of 6 CFU/mL, with satisfactory recoveries in spiked-actual samples.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"283 ","pages":"Article 117558"},"PeriodicalIF":10.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143911648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}