Proximity ligation strategy-mediated cross-modal logic-gate sensing platform: Towards dual biomarkers detection for complex disease

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-07-17 DOI:10.1016/j.bios.2025.117798

Lihong Gao , Xiaofeng Li , Shupei Zhang , Yixuan Li , Guoqing Liu , Xuejun Zhou , Hong Dai

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引用次数: 0

Abstract

The development of a dual-target detection platform with independent signal output can effectively improve the accuracy and confidence of early diagnosis of complex diseases. Herein, a proximity ligation strategy-mediated cross-modal logic-gate sensing platform was constructed with colorimetric and electrochemiluminescence (ECL) as dominant output while photothermal as an auxiliary mode for human epididymis protein 4 (HE4) and ribonuclease A (RNase A) detection. The in situ generated silver nanoparticles (Ag NPs) on MXene (MXene@Ag NPs) and the integration of coreactants (PEI) and luminol by magnetic beads (MBs-PEI-luminol-AD) provides amplified colorimetric and ECL signals to endow excellent sensitivity of the platform. The constructed platform operates AND logic gate for OC detection. The levels of RNase A and HE4 are detected by independent signal outputs from different modes, preventing signal crossover and mutual interference, which ensures the accuracy of the detection. This work provides a suitable model for the construction of cross-modal logic-gate sensing platform, which is expected to be applied in the early diagnosis of complex diseases.

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近距离连接策略介导的跨模态逻辑门传感平台：用于复杂疾病的双重生物标志物检测

开发具有独立信号输出的双靶点检测平台，可有效提高复杂疾病早期诊断的准确性和置信度。本文构建了一个近距离连接策略介导的跨模态逻辑门传感平台，以比色和电化学发光（ECL）为主要输出，光热作为辅助模式检测人附睾蛋白4 （HE4）和核糖核酸酶a （RNase a）。MXene （MXene@Ag NPs）上原位生成的银纳米粒子（Ag NPs）和磁珠（MBs-PEI-luminol-AD）整合的共反应物（PEI）和鲁米诺提供了放大的比色和ECL信号，赋予了平台优异的灵敏度。所构建的平台运行与逻辑门进行OC检测。RNase A和HE4的水平检测采用不同模式的独立信号输出，避免了信号交叉和相互干扰，保证了检测的准确性。本研究为跨模态逻辑门传感平台的构建提供了合适的模型，有望应用于复杂疾病的早期诊断。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.