Proximity ligation strategy-mediated cross-modal logic-gate sensing platform: Towards dual biomarkers detection for complex disease

The development of a dual-target detection platform with independent signal output can effectively improve the accuracy and confidence of early diagnosis of complex diseases. Herein, a proximity ligation strategy-mediated cross-modal logic-gate sensing platform was constructed with colorimetric and electrochemiluminescence (ECL) as dominant output while photothermal as an auxiliary mode for human epididymis protein 4 (HE4) and ribonuclease A (RNase A) detection. The in situ generated silver nanoparticles (Ag NPs) on MXene (MXene@Ag NPs) and the integration of coreactants (PEI) and luminol by magnetic beads (MBs-PEI-luminol-AD) provides amplified colorimetric and ECL signals to endow excellent sensitivity of the platform. The constructed platform operates AND logic gate for OC detection. The levels of RNase A and HE4 are detected by independent signal outputs from different modes, preventing signal crossover and mutual interference, which ensures the accuracy of the detection. This work provides a suitable model for the construction of cross-modal logic-gate sensing platform, which is expected to be applied in the early diagnosis of complex diseases.