用于HIV病毒检测的树突DNA生物传感器。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-09-26 DOI:10.1016/j.bios.2025.118014

Estefanía Enebral Romero , Marta Toldos-Torres , David López-Diego , Mónica Luna , Marta Failde , Brais González-Tobío , Félix Zamora , Iker Falces-Romero , María Luisa Montes , Tania García-Mendiola

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引用次数: 0

摘要

在这项工作中，我们提出了一种创新的电化学DNA生物传感器，用于基于人类免疫缺陷病毒（HIV）的遗传密码的早期，快速，选择性和敏感检测。该平台是基于集成的少层铋（FLB），它提供了一个纳米结构的表面，并促进锚定的DNA树状结构，其中包含生物识别元件（捕获探针补充hiv特异性DNA序列）。使用Azure A （AA）作为氧化还原指示剂检测捕获探针与特定HIV靶序列之间的杂交。树突状DNA生物传感器可选择性检测10.0 fM至10.0 pM范围内的HIV，具有3.03 fM的低检测限和60天的稳定性，并已使用不同病毒载量感染患者的血浆样本进行验证。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Dendrimer-based DNA biosensor for HIV virus detection

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Dendrimer-based DNA biosensor for HIV virus detection

In this work, we propose an innovative electrochemical DNA biosensor for the early, rapid, selective, and sensitive detection of the human immunodeficiency virus (HIV) based on its genetic code. This platform is based on the integration of few-layer bismuthene (FLB) which provides a nanostructured surface and facilitates the anchoring of the DNA dendrimer which incorporates the biorecognition element (a capture probe complementary to the HIV-specific DNA sequence). The hybridization between the capture probe and the specific HIV target sequence is detected using Azure A (AA) as a redox indicator. The dendrimer-based DNA biosensor selectively detects HIV from 10.0 fM to 10.0 pM, exhibit a low detection limit of 3.03 fM and a 60-day stability, and has been validated using plasma samples from infected patients with varying viral loads.

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.