Anti-fouling portable electrochemical device for simultaneous detection of CEA and CA15-3 in serum and nipple discharge

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-09-25 DOI:10.1016/j.bios.2025.118030

Shuqi Chen , Cong Wang , Ting Tang , Xiaoru Zhang , Zehua Wang , Xunyi Yuan

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引用次数: 0

Abstract

Early diagnosis of breast cancer is crucial for enhancing patient survival rates. This study developed a portable electrochemical sensor for simultaneous detection of carcinoembryonic antigen (CEA) and cancer antigen 15-3 (CA15-3). Glutaraldehyde cross-linked denatured bovine serum albumin and polydopamine (PDA) was used as an anti-fouling electrochemical sensor interface for screen-printed electrode (SPE). Etched UiO-66-d was employed to adsorb methylene blue and neutral red dyes, followed by modification with phenylboronic acid (PBA) to produce two redox tags. Aptamer DNA and PBA were used to recognize target CEA and CA15-3 to form sandwich structures. To enhance the Point-of-Care Testing (POCT) performance, a portable dual-channel electrochemical detection device was developed integrated with dual-channel SPE for simultaneous detection of CEA and CA15-3. The detection limits for CEA and CA15-3 were determined to be 0.35 pg/mL and 0.0032 U/mL, respectively. From analyzing both serum and nipple discharge samples, it indicates that using nipple discharge as the detection object can effectively differentiate atypical ductal hyperplasia (a pre-breast cancer lesion) from benign hyperplasia, which holds significant implications for early breast cancer diagnosis.

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同时检测血清和乳头分泌物中CEA和CA15-3的防污便携式电化学装置

乳腺癌的早期诊断对于提高患者的生存率至关重要。本研究研制了一种同时检测癌胚抗原（CEA）和癌抗原15-3 （CA15-3）的便携式电化学传感器。采用戊二醛交联变性牛血清白蛋白-聚多巴胺（PDA）作为丝网印刷电极（SPE）的防污电化学传感器接口。用刻蚀后的UiO-66-d吸附亚甲基蓝和中性红染料，然后用苯硼酸（PBA）改性，得到两种氧化还原标签。适体DNA和PBA分别识别靶CEA和CA15-3，形成夹心结构。为了提高POCT （Point-of-Care Testing）的检测性能，研制了一种与双通道SPE集成的便携式双通道电化学检测装置，用于同时检测CEA和CA15-3。CEA和CA15-3的检出限分别为0.35 pg/mL和0.0032 U/mL。通过对血清和乳头溢液样本的分析，表明以乳头溢液作为检测对象，可以有效区分不典型导管增生（乳腺癌前病变）与良性增生，对乳腺癌早期诊断具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.